Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I1 R1 647 270.1 21258240 98.4% 20918108 94.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 4,006,483 +C coding (183/207 nt) b4554 ← hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,006,4801.C98.3% 1640.1 / 13.6 486coding (186/207 nt)b4554hypothetical protein
Reads supporting (aligned to +/- strand):  ref base . (4/4);  new base C (254/220);  total (262/224)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.93e-01

Only 100 of 523 total aligned reads displayed.

CGGCGCGCCACTGCTGGATTCCCAGCCAAATCAAGTAAGCCGCGCCTGCCCACTTCAACACTTCAAACGCAATCACTGAGCGGGAAAATAGC‑GT‑CCCAACCCCACGCCAACCAGCACAATATGAATCGCCAGTCCGGTCTGAAGCCCAGCAATAGACGCCACCGCGCCGCGATAACCGTGGT  >  NC_000913/4006387‑4006568
                                                                                               |                                                                                        
    gcgcCACTGCTGGATTCCCAGCCAAATCAAGTAAGCCGCGCCTGCCCACTTCAACACTTCAAACGCAATCACTGAGCGGGAAAATAGC‑GT‑cccc                                                                                      <  1:9592921/94‑2 (MQ=255)
    gcgcCACTGCTGGATTCCCAGCCAAATCAAGTAAGCCGCGCCTGCCCACTTCAACACTTCAAACGCAATCACTGAGCGGGAAAATAGC‑GT‑cccc                                                                                      >  2:8730117/1‑93 (MQ=255)
     cgcCACTGCTGGATTCCCAGCCAAATCAAGTAAGCCGCGCCTGCCCACTTCAACACTTCAAACGCAATCACTGAGCGGGAAAATAGC‑GT‑cccca                                                                                     >  2:2065510/1‑92 (MQ=255)
     cgcCACTGCTGGATTCCCAGCCAAATCAAGTAAGCCGCGCCTGCCCACTTCAACACTTCAAACGCAATCACTGAGCGGGAAAATAGC‑GT‑cccca                                                                                     <  1:9738752/94‑3 (MQ=255)
       ccACTGCTGGATTCCCAGCCAAATCAAGTAAGCCGCGCCTGCCCACTTCAACACTTCAAACGCAATCACTGAGCGGGAAAATAGC‑GT‑ccccaac                                                                                   >  2:9453495/1‑90 (MQ=255)
       ccACTGCTGGATTCCCAGCCAAATCAAGTAAGCCGCGCCTGCCCACTTCAACACTTCAAACGCAATCACTGAGCGGGAAAATAGC‑GT‑ccccaac                                                                                   >  2:4453820/1‑90 (MQ=255)
          ctgctgGATTCCCAGCCAAATCAAGTAAGCCGCGCCTGCCCACTTCAACACTTCAAACGCAATCACTGAGCGGGAAAATAGC‑GTCCCCAAcccc                                                                                 <  2:3056708/94‑1 (MQ=255)
          ctgctgGATTCCCAGCCAAATCAAGTAAGCCGCGCCTGCCCACTTCAACACTTCAAACGCAATCACTGAGCGGGAAAATAGC‑GTCCCCAAcccc                                                                                 >  1:4832364/1‑94 (MQ=255)
          ctgctgGATTCCCAGCCAAATCAAGTAAGCCGCGCCTGCCCACTTCAACACTTCAAACGCAATCACTGAGCGGGAAAATAGC‑GTCCCCAAcccc                                                                                 <  2:1223888/94‑1 (MQ=255)
            gctgGATTCCCAGCAAAATCAAGTAAGCCGCGCCTGCCCACTTCAACACTTCAAACGCAATCACTGAGCGGGAAAATAGC‑GTCCCCAACCCCAc                                                                               <  2:7875441/94‑1 (MQ=255)
             ctgGATTCCCAGCCAAATCAAGTAAGCCGCGCCTGCCCACTTCAACACTTCAAACGCAATCACTGAGCGGGAAAATAGC‑GTCCCCAACCCCACg                                                                              <  2:3726551/94‑1 (MQ=255)
             ctgGATTCCCAGCCAAATCAAGTAAGCCGCGCCTGCCCACTTCAACACTTCAAACGCAATCACTGAGCGGGAAAATAGC‑GTCCCCAACCCCACg                                                                              >  1:7670457/1‑94 (MQ=255)
                gATTCCCAGCCAAATCAAGTAAGCCGCGCCTGCCCACTTCAACACTTCAAACGCAATCACTGAGCGGGAAAATAGC‑GTCCCCAACCCCACGCCa                                                                           <  1:1752136/94‑1 (MQ=255)
                gATTCCCAGCCAAATCAAGTAAGCCGCGCCTGCCCACTTCAACACTTCAAACGCAATCACTGAGCGGGAAAATAGC‑GTCCCCAACCCCACGCCa                                                                           >  1:6161514/1‑94 (MQ=255)
                gATTCCCAGCCAAATCAAGTAAGCCGCGCCTGCCCACTTCAACACTTCAAACGCAATCACTGAGCGGGAAAATAGC‑GTCCCCAACCCCACGCCa                                                                           <  2:6231895/94‑1 (MQ=255)
                gATTCCCAGCCAAATCAAGTAAGCCGCGCCTGCCCACTTCAACACTTCAAACGCAATCACTGAGCGGGAAAATAGC‑GTCCCCAACCCCACGCCa                                                                           <  1:1986643/94‑1 (MQ=255)
                 aTTCCCAGCCAAATCAAGTAAGCCGCGCCTGCCCACTTCAACACTTCAAACGCAATCACTGAGCGGGAAAATAGC‑GTCCCCAACCCCACGCCaa                                                                          >  1:9200325/1‑94 (MQ=255)
                 aTTCCCAGCCAAATCAAGTAAGCCGCGCCTGCCCACTTCAACACTTCAAACGCAATCACTGAGCGGGAAAATAGC‑GTCCCCAACCCCACGCCaa                                                                          >  2:5541871/1‑94 (MQ=255)
                         ccAAATCAAGTAAGCCGCGCCTGCCCACTTCAACACTTCAAACGCAATCACTGAGCGGGAAAATAGC‑GTCCCCAACCCCACGCCAACCAGcaca                                                                  >  2:7623947/1‑94 (MQ=255)
                         ccAAATCAAGTAAGCCGCGCCTGCCCACTTCAACACTTCAAACGCAATCACTGAGCGGGAAAATAGC‑GTCCCCAACCCCACGCCAACCAGcaca                                                                  <  1:3913738/94‑1 (MQ=255)
                          cAAATCAAGTAAGCCGCGCCTGCCCACTTCAAAACTTCAAACGCAATCACTGAGCGGGAAAATAGC‑GTCCCCAACCCCACGCCAACCAGCACaa                                                                 <  1:10595120/94‑1 (MQ=255)
                           aaaTGAAGTAAGCCGCGCCTGCCCACTTCAACACTTCAAACGCAATCACTGAGCGGGAAAATAGC‑GTCCCCAACCCCACGCCAACCAGCACAat                                                                <  2:8924586/94‑1 (MQ=255)
                           aaaTCAAGTAAGCCGCCCCTCCCCACTTCAACCCTTCAAACCCCCTCACTGTTCAGGAAAATAGC‑GTCCCCAACCCCACGCCAACCAGCACAat                                                                >  1:1367885/1‑94 (MQ=255)
                            aaTCAAGTAAGCCGCGCCTGCCCACTTCAACACTTCAAACGCAATCACTGAGCGGGAAAATAGC‑GTCCCCAACCCCACGCCAACCAGCACAata                                                               <  1:10083847/94‑1 (MQ=255)
                             aTCAAGTAAGCCGCGCCTGCCCACTTCAACACTTCAAACGCAATCACTGAGCGGGAAAATAGC‑GTCCCCAACCCCACGCCAACCAGCACAatat                                                              <  2:2551166/94‑1 (MQ=255)
                             aTCAAGTAAGCCGCGCCTGCCCACTTCAACACTTCAAACGCAATCACTGAGCGGGAAAATAGC‑GTCCCCAACCCCACGCCAACCAGCACAatat                                                              <  1:1177089/94‑1 (MQ=255)
                             aTCAAGTAAGCCGCGCCTGCCCACTTCAACACTTCAAACGCAATCACTGAGCGGGAAAATAGC‑GTCCCCAACCCCACGCCAACCAGCACAatat                                                              <  1:3207574/94‑1 (MQ=255)
                               cAAGTAAGCCGCGCCTGCCCACTTCAACACTTCAAACGCAATCACTGAGCGGGAAAATAGC‑GTCCCCAACCCCACGCCAACCAGCACAATATGa                                                            <  2:3839685/94‑1 (MQ=255)
                                 aGTAAGCCGCGCCTGCCCACTTCAACACTTCAAACGCAATCACTGAGCGGGAAAATAGC‑GTCCCCAACCCCACGCCAACCAGCACAATATGAAt                                                          <  1:4050857/94‑1 (MQ=255)
                                 aGTAAGCCGCGCCTGCCCACTTCAACACTTCAAACGCAATCACTGAGCGGGAAAATAGC‑GTCCCCAACCCCACGCCAACCAGCACAATATGAAt                                                          >  2:7024151/1‑94 (MQ=255)
                                  gTAAGCCGCGCCTGCCCACTTCAACACTTCAAACGCAATCACTGAGCGGGAAAATAGC‑GTCCCCAACCCCACGCCAACCAGCACAATATGAATc                                                         <  1:891578/94‑1 (MQ=255)
                                  gTAAGCCGCGCCTGCCCACTTCAACACTTCAAACGCAATCACTGAGCGGGAAAATAGC‑GTCCCCAACCCCACGCCAACCAGCACAATATGAATc                                                         <  1:4919956/94‑1 (MQ=255)
                                     aGCCGCGCCTGCCCACTTCAACACTTCAAACGCAATCACTGAGCGGGAAAATAGC‑GTCCCCAACCCCACGCCAACCAGCACAATATGAATCGcc                                                      <  2:10429255/94‑1 (MQ=255)
                                      gCCGCGCCTGCCCACTTCAACACTTCAAACGCAATCACTGAGCGGGAAAATAGC‑GTCCCCAACCCCACGCCAACCAGCACAATATGAATCGCCa                                                     <  1:7169651/94‑1 (MQ=255)
                                      accGCGCCTGCCCACTTCAACACTTCAAACGCAATCACTGAGCGGGAAAATAGC‑GTCCCCAACCCCACGCCAACCAGCACAATATGAATCGCCa                                                     <  1:3082906/93‑1 (MQ=255)
                                         gcgcCTGCCCACTTCAACACTTCAAACGCAATCACTGAGCGGGAAAATAGC‑GTCCCCAACCCCACGCCAACCAGCACAATATGAATCGCCAGTc                                                  >  1:4204167/1‑94 (MQ=255)
                                          cgcCTGCCCACTTCAACACTTCAAACGCAATCACTGAGCGGGAAAATAGC‑GTCCCCAACCCCACGCCAACCAGCACAATATGAATCGCCAGTcc                                                 >  2:4662502/1‑94 (MQ=255)
                                           gcCTGCCCACTTCAACACTTCAAACGCAATCACTGAGCGGGAAAATAGC‑GTCCCCAACCCCACGCCAACCAGCACAATATGAATCGCCAGTCCg                                                >  2:3253495/1‑94 (MQ=255)
                                           gcCTGCCCACTTCAACACTTCAAACGCAATCACTGAGCGGGAAAATAGC‑GTCCCCAACCCCACGCCAACCAGCACAATATGAATCGCCAGTCCg                                                >  2:1761328/1‑94 (MQ=255)
                                           gcCTGCCCACTTCAACACTTCAAACGCAATCACTGAGCGGGAAAATAGC‑GTCCCCAACCCCACGCCAACCAGCACAATATGAATCGCCAGTCCg                                                <  2:5159467/94‑1 (MQ=255)
                                           gcCTGCCCACTTCAACACTTCAAACGCAATCACTGAGCGGGAAAATAGC‑GTCCCCAACCCCACGCCAACCAGCACAATATGAATCGCCAGTCCg                                                >  1:3764908/1‑94 (MQ=255)
                                            ccggCCCACTTCAACACTTCAAACGCAATCACTGAGCGGGAAAATAGC‑GTCCCCAACCCCACGCCAACCAGCACAATATGAATCGCCAGTCCgg                                               <  1:8135338/91‑1 (MQ=255)
                                            ccTGCCCACTTCAACACTTCAAACGCAATCACTGAGCGGGAAAATAGC‑GTCCCCAACCCCACGCCAACCAGCACAATATGAATCGCCAGTCCgg                                               >  1:5891493/1‑94 (MQ=255)
                                            ccTGCCCACTTCAACACTTCAAACGCAATCACTGAGCGGGAAAATAGC‑GTCCCCAACCCCACGCCAACCAGCACAATATGAATCGCCAGTCCgg                                               <  1:7924125/94‑1 (MQ=255)
                                            ccTGCCCACTTCAACACTTCAAACGCAATCACTGAGCGGGAAAATAGC‑GTCCCCAACCCCACGCCAACCAGCACAATATGAATCGCCAGTCCgg                                               >  2:2286389/1‑94 (MQ=255)
                                            ccTGCCCACTTCAACACTTCAAACGCAATCACTGAGCGGGAAAATAGC‑GTCCCCAACCCCACGCCAACCAGCACAATATGAATCGCCAGTCCgg                                               >  1:9226907/1‑94 (MQ=255)
                                             cTGCCCACTTCAACACTTCAAACGCAATCACTGAGCGGGAAAATAGC‑GTCCCCAACCCCACGCCAACCAGCACAATATGAATCGCCAGTCCGGt                                              >  1:7223909/1‑94 (MQ=255)
                                             cTGCCCACTTCAACACTTCAAACGCAATCACTGAGCGGGAAAATAGC‑GTCCCCAACCCCACGCCAACCAGCACAATATGAATCGCCAGTCCGGt                                              >  1:6585071/1‑94 (MQ=255)
                                               gCCCACTTCAACACTTCAAACGCAATCACTGAGCGGGAAAATAGC‑GTCCCCAACCCCACGCCAACCAGCACAATATGAATCGCCAGTCCGGTCt                                            <  1:5256957/94‑1 (MQ=255)
                                               gCCCACTTCAACACTTCAAACGCAATCACTGAGCGGGAAAATAGC‑GTCCCCAACCCCACGCCAACCAGCACAATATGAATCGCCAGTCCGGTCt                                            <  2:185605/94‑1 (MQ=255)
                                               gCCCACTTCAACACTTCAAACGCAATCACTGAGCGGGAAAATAGC‑GTCCCCAACCCCACGCCAACCAGCACAATATGAATCGCCAGTCCGGTCt                                            <  1:2719503/94‑1 (MQ=255)
                                               gCCCACTTCAACACTTCAAACGCAATCACTGAGCGGGAAAATAGC‑GTCCCCAACCCCACGCCAACCAGCACAATATGAATCGCCAGTCCGGTCt                                            <  1:1597089/94‑1 (MQ=255)
                                                cccACTTCAACACTTCAAACGCAATCACTGAGCGGGAAAATAGC‑GTCCCCAACCCCACGCCAACCAGCACAATATGAATCGCCAGTCCGGTCTg                                           <  1:5174533/94‑1 (MQ=255)
                                                 ccACTTCAACACTTCAAACGCAATCACTGAGCGGGAAAATAGC‑GTCCCCAACCCCACGCCAACCAGCACAATATGAATCGCCAGTCCGGTCTGa                                          >  2:5300093/1‑94 (MQ=255)
                                                 ccACTTCAACACTTCAAACGCAATCACTGAGCGGGAAAATAGC‑GTCCCCAACCCCACGCCAACCAGCACAATATGAATCGCCAGTCCGGTCTGa                                          >  1:10198941/1‑94 (MQ=255)
                                                   acttcaacacttcaaACGCAATCACTGAGCGGGAAAATAGC‑GTCCCCAACCCCACGCCAACCAGCACAATATGAATCGCCAGTCCGGTCTGAAg                                        >  1:4764001/1‑94 (MQ=255)
                                                    cttcaacacttcaaACGCAATCACTGAGCGGGAAAATAGC‑GTCCCCAACCCCACGCCAACCAGCACAATATGAATCGCCAGTCCGGTCTGAAGc                                       >  1:9322429/1‑94 (MQ=255)
                                                    cttcaacacttcaaACGCAATCACTGAGCGGGAAAATAGC‑GTCCCCAACCCCACGCCAACCAGCACAATATGAATCGCCAGTCCGGTCTGAAGc                                       >  2:8431004/1‑94 (MQ=255)
                                                     ttcaacacttcaaACGCAATCACTGAGCGGGAAAATAGC‑GTCCCCAACCCCACGCCAACCAGCACAATATGAATCGCCAGTCCGGTCTGAAGcc                                      >  1:5667626/1‑94 (MQ=255)
                                                     ttcaacacttcaaACGCAATCACTGAGCGGGAAAATAGC‑GTCCCCAACCCCACGCCAACCAGCACAATATGAATCGCCAGTCCGGTCTGAAGcc                                      <  1:6360963/94‑1 (MQ=255)
                                                      tcaacacttcaaACGCAATCACTGAGCGGGAAAATAGC‑GTCCCCAACCCCACGCCAACCAGCACAATATGAATCGCCAGTCCGGTCTGAAGccc                                     <  2:1506996/94‑1 (MQ=255)
                                                      tcaacacttcaaACGCAATCACTGAGCGGGAAAATAGC‑GTCCCCAACCCCACGCCAACCAGCACAATATGAATCGCCAGTCCGGTCTGAAGccc                                     <  2:6579029/94‑1 (MQ=255)
                                                      tcaacacttcaaACGCAATCACTGAGCGGGAAAATAGC‑GTCCCCAACCCCACGCCAACCAGCACAATATGAATCGCCAGTCCGGTCTGAAGccc                                     <  2:5985552/94‑1 (MQ=255)
                                                      tcaacacttcaaACGCAATCACTGAGCGGGAAAATAGC‑GTCCCCAACCCCACGCCAACCAGCACAATATGAATCGCCAGTCCGGTCTGAAGccc                                     <  2:8544986/94‑1 (MQ=255)
                                                         acacttcaaACGCAATCACTGAGCGGGAAAATAGC‑GTCCCCAACCCCACGCCAACCAGCACAATATGAATCGCCATTCCGGTCTGAAGCCCAga                                  >  2:5704854/1‑93 (MQ=255)
                                                          cacttcaaACGCAATCACTGAGCGGGAAAATAGC‑GTCCCCAACCCCACGCCAACCAGCACAATATGAATCGCCAGTCCGGTCTGAAGCCCAGCa                                 <  2:2752466/94‑1 (MQ=255)
                                                           acttcaaACGCAATCACTGAGCGGGAAAATAGC‑GTCCCCAACCCCACGCCAACCAGCACAATATGAATCGCCAGTCCGGTCTGAAGCCCAGCaa                                <  2:4096700/94‑1 (MQ=255)
                                                            cttcaaACGCAATCACTGAGCGGGAAAATAGC‑GTCCCCAACCCCACGCCAACCAGCACAATATGAATCGCCAGTCCGGTCTGAAGCCCAGCAAt                               >  2:7544851/1‑94 (MQ=255)
                                                            cttcaaACGCAATCACTGAGCGGGAAAATAGC‑GTCCCCAACCCCACGCCAACCAGCACAATATGAATCGCCAGTCCGGTCTGAAGCCCAGCAAt                               >  2:7694648/1‑94 (MQ=255)
                                                              tcaaACGCAATCACTGAGCGGGAAAATAGC‑GTCCCCAACCCCACGCCAACCAGCACAATATGAATCGCCAGTCCGGTCTGAAGCCCAGCAATAg                             <  1:5457401/94‑1 (MQ=255)
                                                              tcaaACGCAATCACTGAGCGGGAAAATAGC‑GTCCCCAACCCCACGCCAACCAGCACAATATGAATCGCCAGTCCGGTCTGAAGCCCAGCAATAg                             <  1:7783895/94‑1 (MQ=255)
                                                               caaACGCAATCACTGAGCGGGAAAATAGC‑GTCCCCAACCCCACGCCAACCAGCACAATATGAATCGCCAGTCCGGTCTGAAGCCCAGCAATAGa                            >  1:1539765/1‑94 (MQ=255)
                                                                aaaCGCAATCACTGAGCGGGAAAATCGCGGC‑CCCAACCCCACGCCAACCAGCACAATATGAATCGCCAGTCCGGTCTGAAGCCCAGCAATAGAc                           <  1:3453178/94‑1 (MQ=255)
                                                                aaaCGCAATCACTGAGCGGGAAAATAGC‑GTCCCCAACCCCACGCCAACCAGCACAATATGAATCGCCAGTCCGGTCTGAAGCCCAGCAATAGAc                           <  1:5078806/94‑1 (MQ=255)
                                                                aaaCGCAATCACTGAGCGGGAAAATAGC‑GTCCCCAACCCCACGCCAACCAGCACAATATGAATCGCCAGTCCGGTCTGAAGCCCAGCAATAGAc                           <  2:3419044/94‑1 (MQ=255)
                                                                 aaCGCAATCACTGAGCGGGAAAATAGC‑GTCCCCAACCCCACGCCAACCAGCACAATATGAATCGCCAGTCCGGTCTGAAGCCCAGCAATAGACg                          <  1:7406342/94‑1 (MQ=255)
                                                                   cGCAATCACTGAGCGGGAAAATAGC‑GTCCCCAACCCCACGCCAACCAGCACAATATGAATCGCCAGTCCGGTCTGAAGCCCAGCAATAGACGcc                        >  2:928594/1‑94 (MQ=255)
                                                                    gCAATCACTGAGCGGGAAAATAGC‑GTCCCCAACCCCACGCCAACCAGCACAATATGAATCGCCAGTCCGGTCTGAAGCCCAGCAATAGACGCCa                       <  1:2638966/94‑1 (MQ=255)
                                                                     cAATCACTGAGCGGGAAAATAGC‑GTCCCCAACCCCACGCCAACCAGCACAATATGAATCGCCAGTCCGGTCTGAAGCCCAGCAATAGACGCCAc                      >  2:2908107/1‑94 (MQ=255)
                                                                      caTCACTGAGCGGGAAAATAGC‑GTCCCCAACCCCACGCCAACCAGCACAATATGAATCGCCAGTCCGGTCTGAAGCCCAGCAATAGACGCCAcc                     <  2:1097764/93‑1 (MQ=255)
                                                                       aTCACTGAGCGGGAAAATAGC‑GTCCCCAACCCCACGCCAACCAGCACAATATGAATCGCCAGTCCGGTCTGAAGCCCAGCAATAGACGCCAccg                    >  2:8207860/1‑94 (MQ=255)
                                                                       aTCACTGAGCGGGAAAATAGC‑GTCCCCAACCCCACGCCAACCAGCACAATATGAATCGCCAGTCCGGTCTGAAGCCCAGCAATAGACGCCAccg                    <  1:21349/94‑1 (MQ=255)
                                                                        tCACTGAGCGGGAAAATAGC‑GTCCCCAACCCCACGCCAACCAGCACAATATGAATCGCCAGTCCGGTCTGAAGCCCAGCAATAGACGCCAccgc                   <  1:7012589/94‑1 (MQ=255)
                                                                        tCACTGAGCGGGAAAATAGC‑GTCCCCAACCCCACGCCAACCAGCACAATATGAATCGCCAGTCCGGTCTGAAGCCCAGCAATAGACGCCAccgc                   <  1:10479926/94‑1 (MQ=255)
                                                                         cACTGAGCGGGAAAATAGC‑GTCCCCAACCCCACGCCAACCAGCACAATATGAATCGCCAGTCCGGTCTGAAGCCCAGCAATAGACGCCAccgcg                  <  1:3560353/94‑1 (MQ=255)
                                                                          aCTGAGCGGGAAAATAGC‑GTCCCCAACCCCACGCCAACCAGCACAATATGAATCGCCAGTCCGGTCTGAAGCCCAGCAATAGACGCCAccgcgc                 >  2:977022/1‑94 (MQ=255)
                                                                           cTGAGCGGGAAAATAGC‑GTCCCCAACCCCACGCCAACCAGCACAATATGAATCGCCAGTCCGGTCTGAAGCCCAGCAATAGACGCCAccgcgcc                >  2:4375037/1‑94 (MQ=255)
                                                                            tGAGCGGGAAAATAGC‑GTCCCCAACCCCACGCCAACCAGCACAATATGAATCGCCAGTCCGGTCTGAAGCCCAGCAATAGACGCCAccgcgccg               >  2:5468752/1‑94 (MQ=255)
                                                                            tGAGCGGGAAAATAGC‑GTCCCCAACCCCACGCCAACCAGCACAATATGAATCGCCAGTCCGGTCTGAAGCCCAGCAATAGACGCCAccgcgccg               >  2:4847295/1‑94 (MQ=255)
                                                                                cGGGAAAATAGC‑GTCCCCAACCCCACGCCAACCAGCACAATATGAATCGCCAGTCCGGTCTGAAGCCCAGCAATAGACGCCAccgcgccgcgca           >  1:5364081/1‑92 (MQ=255)
                                                                                 gggAAAATAGC‑GTCCCCAACCCCACGCCAACCAGCACAATATGAATCGCCAGTCCGGTCTGAAGCCCAGCAATAGACGCCACCGCGCCGCGATa          >  2:6790489/1‑94 (MQ=255)
                                                                                   gAAAATAGC‑GTCCCCAACCCCACGCCAACCAGCACAATATGAATCGCCAGTCCGGTCTGAAGCCCAGCAATAGACGCCACCGCGCCGCGATAAc        <  1:6887742/94‑1 (MQ=255)
                                                                                   gAAAATAGC‑GTCCCCAACCCCACGCCAACCAGCACAATATGAATCGCCAGTCCGGTCTGAAGCCCAGCAATAGACGCCACCGCGCCGCGATAAc        >  1:234384/1‑94 (MQ=255)
                                                                                    aaaaTAGC‑GTCCCCAACCCCACGCCAACCAGCACAATATGAATCGCCAGTCCGGTCTGAAGCCCAGCAATAGACGCCACCGCGCCGCGATAAcc       >  1:8385908/1‑94 (MQ=255)
                                                                                    aaaaTAGC‑GTCCCCAACCCCACGCCAACCAGCACAATATGAATCGCCAGTCCGGTCTGAAGCCCAGCAATAGACGCCACCGCGCCGCGATAAcc       <  1:8654463/94‑1 (MQ=255)
                                                                                    aaaaTAGC‑GTCCCCAACCCCACGCCAACCAGCACAATATGAATCGCCAGTCCGGTCTGAAGCCCAGCAATAGACGCCACCGCGCCGCGATAAcc       >  1:8970180/1‑94 (MQ=255)
                                                                                    aaaaTACC‑GTCCCCAACCCCACGACAACCAGCACAATATGAATCGCCAGTCCGGCCTGAAGCCCAGCAATAGACGCCACCGCGCCGCGATAAcc       <  2:7255742/94‑1 (MQ=255)
                                                                                      aaTAGC‑GTCCCCAACCCCACGCCAACCAGCACAATATGAATCGCCAGTCCGGTCTGAAGCCCAGCAATAGACGCCACCGCGCCGCGATAACCgg     >  2:8056733/1‑93 (MQ=255)
                                                                                        tAGC‑GTCCCCAACCCCACGCCAACCAGCACAATATGAATCGCCAGTCCGGTCTGAAGCCCAGCAATAGACGCCACCGCGCCGCGATAACCGTgg   >  2:4197355/1‑94 (MQ=255)
                                                                                        tAGC‑GTCCCCAACCCCACGCCAACCAGCACAATATGAATCGCCAGTCCGGTCTGAAGCCCAGCAATAGACGCCACCGCGCCGCGATAACCGTgg   >  2:10151391/1‑94 (MQ=255)
                                                                                               |                                                                                        
CGGCGCGCCACTGCTGGATTCCCAGCCAAATCAAGTAAGCCGCGCCTGCCCACTTCAACACTTCAAACGCAATCACTGAGCGGGAAAATAGC‑GT‑CCCAACCCCACGCCAACCAGCACAATATGAATCGCCAGTCCGGTCTGAAGCCCAGCAATAGACGCCACCGCGCCGCGATAACCGTGGT  >  NC_000913/4006387‑4006568

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 19 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output Only 200 of 312 total aligned reads displayed.
CTCCGCACCGCCGCGGGCCATCCTGTCGAAGGAGGACGGCCGTTGGTAATTAAAGGTGCTTACCATGAGATCCTTTTTGAAAAGGACGCAATGGCCTCAGTCGCGCTCCACGCCATCGTTGATTTTTTCAACAGGCATAACTCACCCAGCGGAAACCGCTCTACAGAGGTTTAAATTTCTTAT  >  NC_000913/4007625‑4007807
                                                                                             |                                                                                         
CTCCGCACCGCCGCGGGCCATCCTGTCGAAGGAGGACGGCCGTTGGTAATTAAAGGTGCTTACCATGAGATCCTTTTTGAAAAGGACGCAATGC                                                                                           <  SRR4302545.6863830/94‑1 (MQ=60)
CTCCGCACCGCCGCGGGCCATCCTGTCGAAGGAGGACGGCCGTTGGTAATTAAAGGTGCTTACCATGAGATCCTTTTTGAAAAGGACGCAATGC                                                                                           >  SRR4302545.2910970/1‑94 (MQ=60)
CTCCGCACCGCCGCGGGCCATCCTGTCGAAGGAGGACGGCCGTTGGTAATTAAAGGTGCTTACCATGAGATCCTTTTTGAAAAGGACGCAATGC                                                                                           <  SRR4302545.4505701/94‑1 (MQ=60)
CTCCGCACCGCCGCGGGCCATCCTGTCGAAGGAGGACGGCCGTTGGTAATTAAAGGTGCTTACCATGAGATCCTTTTTGAAAAGGACGCAATGC                                                                                           >  SRR4302545.807663/1‑94 (MQ=60)
CTCCGCACCGCCGCGGGCCATCCTGTCGAAGGAGGACGGCCGTTGGTAATTAAAGGTGCTTACCATGAGATCCTTTTTGAAAAGGACGCAATGC                                                                                           >  SRR4302545.4718790/1‑94 (MQ=60)
CTCCGCACCGCCGCGGGCCATCCTGTCGAAGGAGGACGGCCGTTGGTAATTAAAGGTGCTTACCATGAGATCCTTTTTGAAAAGGACGCAATGC                                                                                           <  SRR4302545.9417616/94‑1 (MQ=60)
     CACCGCCGCGGGCCATCCTGTCGAAGGAGGACGGCCGTTGGTAATTAAAGGTGCTTACCATGAGATCCTTTTTGAAAAGGACGCAATGCGCTCA                                                                                      <  SRR4302545.3009201/94‑1 (MQ=60)
     CACCGCCGCGGGCCATCCTGTCGAAGGAGGACGGCCGTTGGTAATTAAAGGTGCTTACCATGAGATCCTTTTTGAAAAGGACGCAATGCGCTCA                                                                                      >  SRR4302545.6388716/1‑94 (MQ=60)
      CCCGCCGCGGGCCATCCTGTCGAAGGAGGACGGCCGTTGGTAATTAAAGGTGCTTACCATGAGATCCTTTTTGAAAAGGACGCAATGCGCTCAG                                                                                     <  SRR4302545.9089579/94‑1 (MQ=60)
      ACCGCCGCGGGCCATCCTGTCGAAGGAGGACGGCCGTTGGTAATTAAAGGTGCTTACCATGAGATCCTTTTTGAAAAGGACGCAATGCGCTCAG                                                                                     >  SRR4302545.3318875/1‑94 (MQ=60)
      ACCGCCGCGGGCCATCCTGTCGAAGGAGGACGGCCGTTGGTAATTAAAGGTGCTTACCATGAGATCCTTTTTGAAAAGGACGCAATGCGCTCAG                                                                                     <  SRR4302545.5581993/94‑1 (MQ=60)
      ACCGCCGCGGGCCATCCTGTCGAAGGAGGACGGCCGTTGGTAATTAAAGGTGCTTACCATGAGATCCTTTTTGAAAAGGACGCAATGCGCTCAG                                                                                     >  SRR4302545.4405808/1‑94 (MQ=60)
       CCGCCGCGGGCCATCCTGTCGAAGGAGGACGGCCGTTGGTAATTAAAGGTGCTTACCATGAGATCCTTTTTGAAAAGGACGCAATGCGCTCAGT                                                                                    >  SRR4302545.9829413/1‑94 (MQ=60)
       CCGCCGCGGGCCATCCTGTCGAAGGAGGACGGCCGTTGGTAATTAAAGGTGCTTACCATGAGATCCTTTTTGAAAAGGACGCAATGCGCTCAGT                                                                                    <  SRR4302545.7149378/94‑1 (MQ=60)
        CGCCGCGGGCCATCCTGTCGAAGGAGGACGGCCGTTGGTAATTAAAGGTGCTTACCATGAGATCCTTTTTGAAAAGGACGCAATGCGCTCAGTC                                                                                   >  SRR4302545.3826238/1‑94 (MQ=60)
        CGCCGCGGGCCATCCTGTCGAAGGAGGACGGCCGTTGGTAATTAAAGGTGCTTACCATGAGATCCTTTTTGAAAAGGACGCAATGCGCTCAGTC                                                                                   >  SRR4302545.8953038/1‑94 (MQ=60)
        CGCCGCGGGCCATCCTGTCGAAGGAGGACGGCCGTTGGTAATTAAAGGTGCTTACCATGAGATCCTTTTTGAAAAGGACGCAATGCGCTCAGTC                                                                                   >  SRR4302545.6680712/1‑94 (MQ=60)
        CGCCGCGGGCCATCCTGTCGAAGGAGGACGGCCGTTGGTAATTAAAGGTGCTTACCATGAGATCCTTTTTGAAAAGGACGCAATGCGCTCAGTC                                                                                   <  SRR4302545.10389293/94‑1 (MQ=60)
         GCCGCGGGCCATCCTGTCGAAGGAGGACGGCCGTTGGTAATTAAAGGTGCTTACCATGAGATCCTTTTTGAAAAGGACGCAATGCGCTCAGTCG                                                                                  >  SRR4302545.6253630/1‑94 (MQ=60)
         GCCGCGGGCCATCCTGTCGAAGGAGGACGGCCGTTGGTAATTAAAGGTGCTTACCATGAGATCCTTTTTGAAAAGGACGCAATGCGCTCAGTCG                                                                                  >  SRR4302545.10821517/1‑94 (MQ=60)
          CCGCGGGCCATCCTGTCGAAGGAGGACGGCCGTTGGTAATTAAAGGTGCTTACCATGAGATCCTTTTTGAAAAGGACGCAATGCGCTCAGTCGC                                                                                 >  SRR4302545.8744865/1‑94 (MQ=60)
            GCGGGCCATCCTGTCGAAGGAGGACGGCCGTTGGTAATTAAAGGTGCTTACCATGAGATCCTTTTTGAAAAGGACGCAATGCGCGCAGTCGCGC                                                                               >  SRR4302545.8262534/1‑94 (MQ=60)
              GGGCCATCCTGTCGAAGGAGGACGGCCGTTGGTAATTAAAGGTGCTTACCATGAGATCCTTTTTGAAAAGGACGCAATGCGCTCAGTCGCGCTC                                                                             >  SRR4302545.4101234/1‑94 (MQ=60)
              GGGCCATCCTGTCGAAGGAGGACGGCCGTTGGTAATTAAAGGTGCTTACCATGAGATCCTTTTTGAAAAGGACGCAATGCGCTCAGTCGCGCTC                                                                             >  SRR4302545.10655528/1‑94 (MQ=60)
              GGGCCATCCTGTCGAAGGAGGACGGCCGTTGGTAATTAAAGGAGCTTACCATGAGATCCTTTTTGAAAAGGACGCAATGCGCTCAGTCGCGCTC                                                                             >  SRR4302545.3999660/1‑94 (MQ=60)
               GGCCATCCTGTCGAAGGAGGACGGCCGTTGGTAATTAAAGGTGCTTACCATGAGATCCTTTTTGAAAAGGACGCAATGCGCTCAGTCGCGCTCC                                                                            <  SRR4302545.1856965/94‑1 (MQ=60)
                GCCATCCTGTCGAAGGAGGACGGCCGTTGGTAATTAAATGTGCTTACCATGAGATCCTTTTTGAAAAGGACGCAATGCGCTCAGTCGCGCTCCA                                                                           <  SRR4302545.1897714/94‑1 (MQ=60)
                GCCATCCTGTCGAAGGAGGACGGCCGTTGGTAATTAAAGGTGCTTACCATGAGATCCTTTTTGAAAAGGACGCAATGCGCTCAGTCGCGCTCCA                                                                           >  SRR4302545.7761318/1‑94 (MQ=60)
                GCCATCCTGTCGAAGGAGCACGGCCGTTGGTAATTAAAGGTGCTTACCATGAGATCCTTTTTGAAAAGGACGCAATGCGCTCAGTCGCGCTCCA                                                                           <  SRR4302545.6529485/94‑1 (MQ=60)
                 CTATCATGTCGAAGGAGGACGGCCGTTGGTAATTAAAGGTGCTTACCATGAGATCCTTTTTGAAAAGGACGCAATGCGCTCAGTCGCGCTCCAC                                                                          <  SRR4302545.7061185/94‑1 (MQ=60)
                 CCATCCTGTCGAAGGAGGACGGCCGTTGGTAATTAAAGGTGCTTACCATGAGATCCTTTTTGAAAAGGACGCAATGCGCTCAGTCGCGCTCCAC                                                                          <  SRR4302545.274592/94‑1 (MQ=60)
                 CCATCCTGTCGAAGGAGGACGGCCGTTGGTAATTAAAGGTGCTTACCATGAGATCCTTTTTGAAAAGGACGCAATGCGCTCAGTCGCGCTCCAC                                                                          <  SRR4302545.3988885/94‑1 (MQ=60)
                 CCATCCTGTCGAAGGAGGACGGCCGTTGGTAATTAAAGGTGCTTACCATGAGATCCTTTTTGAAAAGGACGCAATGCGCTCAGTCGCGCTCCAC                                                                          <  SRR4302545.835737/94‑1 (MQ=60)
                  CATCCTGTCGAAGGAGGACGGCCGTTGGTAATTAAAGGTGCTTACCATGAGATCCTTTTTGAAAAGGACGCAATGCGCTCAGTCGCGCTCCACG                                                                         <  SRR4302545.5951693/94‑1 (MQ=60)
                  CATCCTGTCGAAGGAGGACGGCCGTTGGTAATTAAAGGTGCTTACCATGAGATCCTTTTTGAAAAGGACGCAATGCGCTCAGTCGCGCTCCACG                                                                         >  SRR4302545.10394500/1‑94 (MQ=60)
                  CATCCTGTCGAAGGAGGACGGCCGTTGGTAATTAAAGGTGCTTACCATGAGATCCTTTTTGAAAAGGACGCAATGCGCTCAGTCGCGCTCCACG                                                                         >  SRR4302545.4426621/1‑94 (MQ=60)
                  CATCCTGTCGAAGGAGGACGGCCGTTGGTAATTAAAGGTGCTTACCATGAGATCCTTTTTGAAAAGGACGCAATGCGCTCAGTCGCGCTCCACG                                                                         <  SRR4302545.10199818/94‑1 (MQ=60)
                  CATCCTGTCGAAGGAGGACGGCCGTTGGTAATTAAAGGTGCTTACCATGAGATCCTTTTTGAAAAGGACGCAATGCGCTCAGTCGCGCTCCACG                                                                         <  SRR4302545.5951694/94‑1 (MQ=60)
                   ATCCTGTCGAAGGAGGACGGCCGTTGGTAATTAAAGGTGCTTACCATGAGATCCTTTTTGAAAAGGACGCAATGCGCTCAGTCGCGCTCCACGC                                                                        >  SRR4302545.6339304/1‑94 (MQ=60)
                   ATCCTGTCGAAGGAGGACGGCCGTTGGTAATTAAAGGTGCTTACCATGAGATCCTTTTTGAAAAGGACGCAATGCGCTCAGTCGCGCTCCACGC                                                                        <  SRR4302545.9545633/94‑1 (MQ=60)
                   ATCCTGTCGAAGGAGGACGGCCGTTGGTAATTAAAGGTGCTTACCATGAGATCCTTTTTGAAAAGGACGCAATGCGCTCAGTCGCGCTCCACGC                                                                        >  SRR4302545.4929866/1‑94 (MQ=60)
                    TCCTGTCGAAGGAGGACGGCCGTTGGTAATTAAAGGTGCTTACCATGAGATCCTTTTTGAAAAGGACGCAATGCGCTCAGTCGCGCTCCACGCC                                                                       >  SRR4302545.6999927/1‑94 (MQ=60)
                     CCTGTCGAAGGAGGACGGCCGTTGGTAATTAAAGGTGCTTACCATGAGATCCTTTTTGAAAAGGACGCAATGCGCTCAGTCGCGCTCCACGCCA                                                                      >  SRR4302545.4751572/1‑94 (MQ=60)
                      CTGTCGAAGGAGGACGGCCGTTGGTAATTAAAGGTGCTTACCATGAGATCCTTTTTGAAAAGGACGCAATGCGCTCAGTCGCGCTCCACGCCAT                                                                     >  SRR4302545.7952046/1‑94 (MQ=60)
                      CTGTCGAAGGAGGACGGCCGTTGGTAATTAAAGGTGCTTACCATGAGATCCTTTTTGAAAAGGACGCAATGCGCTCAGTCGCGCTCCACGCCAT                                                                     >  SRR4302545.7610078/1‑94 (MQ=60)
                      CTGGCGAAGGAGGACGGCCGTTGGTAATTAAAGGTGCTTACCATGAGATCCTTTTTGAAAAGGACGCAATGCGCTCAGTCGCGCTCCACGCCAT                                                                     <  SRR4302545.9458785/94‑1 (MQ=60)
                       TGTCGAAGGAGGACGGCCGTTGGTAATTAAAGGTGCTTACCATGAGATCCTTTTTGAAAAGGACGCAATGCGCTCAGTCGCGCTCCACGCCATC                                                                    >  SRR4302545.673756/1‑94 (MQ=60)
                       TGTCGAAGGAGGACGGCCGTTGGTAATTAAAGGTGATTACCATGAAATCCTTTTTGAAAAGGACGCAATGCGCTCAGTCGCGCTCCACGCCATC                                                                    >  SRR4302545.9745373/1‑94 (MQ=60)
                        GTCGAAGGAGGACGGCCGTTGGTAATTAAAGGTGCTTACCATGAGATCCTTTTTGAAAAGGACGCAATGCGCTCAGTCGCGCTCCACGCCATCG                                                                   <  SRR4302545.5715781/94‑1 (MQ=60)
                        GTCGAAGGAGGACGGCCGTTGGTAATTAAAGGTGCTTACCATGAGATCCTTTTTGAAAAGGACGCAATGCGCTCAGTCGCGCTCCACGCCATCG                                                                   >  SRR4302545.6269371/1‑94 (MQ=60)
                         TCGAAGGAGGACGGCCGTTGGTAATTAAAGGTGCTTACCATGAGATCCTTTTTGAAAAGGACGCAATGCGCTCAGTCGCGCTCCACGCCATCGT                                                                  <  SRR4302545.2568340/94‑1 (MQ=60)
                          CGAAGGAGGACGGCCGTTGGTAATTAAAGGTGCTTACCATGAGATCCTTTTTGAAAAGGACGCAATGCGCTCAGTCGCGCTCCACGCCATCGTT                                                                 >  SRR4302545.9505858/1‑94 (MQ=60)
                          CGAAGGAGGACGGCCGTTGGTAATGAAAGGTGCTTACCATGAGATCCTTTTTGAAAAGGACGCAATGCGCTCAGTCGCGCTCCACGCCATCGTT                                                                 <  SRR4302545.1524116/94‑1 (MQ=60)
                            AAGGAGGACGGCCGTTGGTAATTAAAGGTGCTTACCATGAGATCCTTTTTGAAAAGGACGCAATGCGCTCAGTCGCGCTCCACGCCATCTTTGA                                                               <  SRR4302545.1235315/94‑1 (MQ=60)
                             AGGAGGACGGCCGTTGGTAATTAAAGGTGCTTACCATGAGATCCTTTTTGAAAAGGACGCAATGCGCTCAGTCGCGCTCCACGCCATCGTTGAT                                                              >  SRR4302545.695364/1‑94 (MQ=60)
                             AGGAGGACGGCCGTTGGTAATTAAAGGTGCTTACCATGAGATCCTTTTTGAAAAGGACGCAATGCGCTCAGTCGCGCTCCACGCCATCGTTGAT                                                              >  SRR4302545.4888484/1‑94 (MQ=60)
                              GGAGGACGGCCGTTGGTAATTAAAGGTGCTTACCATGAGATCCTTTTTGAAAAGGACGCAATGCGCTCAGTCGCGCTCCACGCCATCGTTGATT                                                             <  SRR4302545.725475/94‑1 (MQ=60)
                               GAGGACGGCCGTTGGTAATTAAAGGTGCTTACCATGAGATCCTTTTTGAAAAGGACGCAATGCGCTCAGTCGCGCTCCACGCCATCGTTGATTT                                                            >  SRR4302545.4088724/1‑94 (MQ=60)
                               GAGGACGGCCGTTGGTAATTAAAGGTGCTTACCATGAGATCCTTTTTGAAAAGGACGCAATGCGCTCAGTCGCGCTCCACGCCATCGTTGATTT                                                            >  SRR4302545.10722939/1‑94 (MQ=60)
                               GAGGACGGCCGTTGGTAATTAAAGGTGCTTACCATGAGATCCTTTTTGAAAAGGACGCAATGCGCTCAGTCGCGCTCCACGCCATCGTTGATTT                                                            >  SRR4302545.4140310/1‑94 (MQ=60)
                                AGGACGGCCGTTGGTAATTAAAGGTGCTTACCATGAGATCCTTTTTGAAAAGGACGCAATGCGCTCAGTCGCGCTCCACGCCATCGTTGATTTT                                                           <  SRR4302545.4631139/94‑1 (MQ=60)
                                AGGACGGCCGTTGGTAATTAAAGGTGCTTACCATGAGATCCTTTTTGAAAAGGACGCAATGCGCTCAGTCGCGCTCCACGCCATCGTTGATTTT                                                           <  SRR4302545.6914404/94‑1 (MQ=60)
                                 GGACGGCCGTTGGTAATTAAAGGTGCTTACCATGAGATCCTTTTTGAAAAGGACGCAATGCGCTCAGTCGCGCTCCACGCCATCGTTGATTTTT                                                          <  SRR4302545.6001592/94‑1 (MQ=60)
                                 GGACGGCCGTTGGTAATTAAAGGTGCTTACCATGAGATCCTTTTTGAAAAGGACGCAATGCGCTCAGTCGCGCTCCACGCCATCGTTGATTTTT                                                          <  SRR4302545.6763698/94‑1 (MQ=60)
                                 GGACGGCCGTTGGGAATTAAAGGTGTTTACCATGAGATCCTTCTTCAAAACGCAGCAATGCGCTCAGTCGCGCTCCACGCCATCGTTGATTTGT                                                          >  SRR4302545.9766644/1‑94 (MQ=48)
                                 GAACGGCCGTGGATATTTAAAGGTGCTTACCATGAGATCCTTTTTGAAAAGGACGCAATGCGCTCAGTCGCGCTCCCCGCCATCGTTGATTTTT                                                          <  SRR4302545.6763703/94‑1 (MQ=60)
                                  GACGGCCGTTGGTAATTAAAGGTGCTTACCATGAGATCCTTTTTGAAAAGGACGCAATGCGCTCAGTCGCGCTCCACGCCATCGTTGATTTTTT                                                         >  SRR4302545.3369079/1‑94 (MQ=60)
                                  GACGGCCGTTGGTAATTAAAGGTGCTTACCATGAGATCCTTTTTGAAAAGGACGCAATGCGCTCAGTCGCGCTCCACGCCATCGTTGATTTTTT                                                         <  SRR4302545.6001447/94‑1 (MQ=60)
                                   ACGGCCGTTGGTAATTAAAGGTGCTTACCATGAGATCCTTTTTGAAAAGGACGCAATGCGCTCAGTCGCGCTCCACGCCATCGTTGATTTTTTC                                                        <  SRR4302545.8712531/94‑1 (MQ=60)
                                    CGGCCGTTGGTAATTAAAGGTGCTTACCATGAGATCCTTTTTGAAAAGGACGCAATGCGCTCAGTCGCGCTCCACGCCATCGTTGATTTTTTCA                                                       >  SRR4302545.4696208/1‑94 (MQ=60)
                                    CGGCCGTTGGTAATTAAAGGTGCTTACCATGAGATCCTTTTTGAAAAGGACGCAATGCGCTCAGTCGCGCTCCACGCCATCGTTGATTTTTTCA                                                       >  SRR4302545.3919488/1‑94 (MQ=60)
                                     GGCCGTTGGTAATTAAAGGTGCTTACCATGAGATCCTTTTTGAAAAGGACGCAATGCGCTCAGTCGCGCTCCACGCCATCGTTGATTTTTTCAA                                                      >  SRR4302545.5216352/1‑94 (MQ=60)
                                     GGCCGTTGGTAATTAAAGGTGCTTACCATGAGATCCTTTTTGAAAAGGACGCAATGCGCTCAGTCGCGCTCCACGCCATCGTTGATTTTTTCAA                                                      >  SRR4302545.8798882/1‑94 (MQ=60)
                                      GCCGTTGGTAATTAAAGGTGCTTACCATGAGATCCTTTTTGAAAAGGACGCAATGCGCTCAGTCGCGCTCCACGCCATCGTTGATTTTTTCAAC                                                     <  SRR4302545.8529520/94‑1 (MQ=60)
                                       CCGTTGGTAATTAAAGGTGCTTACCATGAGCTCCTTTTTGAAAAGGACGCAATGCGCTCAGTCGCGCTCCACGCCATCGTTGATTTTTTCAACA                                                    >  SRR4302545.2990428/1‑94 (MQ=60)
                                       CCGTTGGTAATTAAAGGTGCTTACCATGAGATCCTTTTTGAAAAGGACGCAATGCGCTCAGTCGCGCTCCACGCCATCGTTGATTTTTTCAACA                                                    >  SRR4302545.4748177/1‑94 (MQ=60)
                                        CGTTGGTAATTAAAGGTGCTTACCATGAGATCCTTTTTGAAAAGGACGCAATGCGCTCAGTCGCGCTCCACGCCATCGTTGATTTTTTCAACAG                                                   >  SRR4302545.4177245/1‑94 (MQ=60)
                                         GTTGGTAATTAAAGGTGCTTACCATGAGATCCTTTTTGAAAAGGACGCAATGCGCTCAGTCGCGCTCCACGCCATCGTTGATTTTTTCAACAGG                                                  >  SRR4302545.1612974/1‑94 (MQ=60)
                                           TGGTAATTAAAGGTGCTTACCATGAGATCCTTTTTGAAAAGGACGCAATGCGCTCAGTCGCGCTCCACGCCATCGTTGATTTTTTCAACAGGCA                                                <  SRR4302545.1909227/94‑1 (MQ=60)
                                           TGGTAATTAAAGGTGCTTACCATGAGATCCTTTTTGAAAAGGACGCAATGCGCTCAGTCGCGCTCCACGCCATCGTTGATTTTTTCAACAGGCA                                                >  SRR4302545.2117199/1‑94 (MQ=60)
                                           TGGTAATTAAAGGTGCTTACCATGAGATCCTTTTTGAAAAGGACGCAATGCGCTCAGTCGCGCTCCACGCCATCGTTGATTTTTTCAACAGGCA                                                <  SRR4302545.5796899/94‑1 (MQ=60)
                                           TGGTAATTAAAGGTGCTTACCATGAGATCCTTTTTGAAAAGGACGCAATGCGCTCAGTCGCGCTCCACGCCATCGTTGATTTTTTCAACAGGCA                                                >  SRR4302545.8920951/1‑94 (MQ=60)
                                            GGTAATTAAAGGTGCTTACCATGAGATCCTTTTTGAAAAGGACGCAATGCGCTCAGTCGCGCTCCACGCCATCGTTGATTTTTTCAACAGGCAT                                               >  SRR4302545.2928982/1‑94 (MQ=60)
                                            GGTAATTAAAGGTGCTTACCATGAGATCCTTTTTGAAAAGGACGCAATGCGCTCAGTCGCGCTCCACGCCATCGTTGATTTTTTCAACAGGCAT                                               >  SRR4302545.10341520/1‑94 (MQ=60)
                                            GGTAATTAAAGGTGCTTACCATGAGATCCTTTTTGAAAAGGACGCAATGCGCTCAGTCGCGCTCCACGCCATCGTTGATTTTTTCAACAGGCAT                                               >  SRR4302545.6030537/1‑94 (MQ=60)
                                             GTAATTAAAGGTGCTTACCATGAGATCCTTTTTGAAAAGGACGCAATGCGCTCAGTCGCGCTCCACGCCATCGTTGATTTTTTCAACAGGCATA                                              >  SRR4302545.4938127/1‑94 (MQ=60)
                                             GTAATTAAAGGTGCTTACCATGAGATCCTTTTTGAAAAGGACGCAATGCGCTCAGTCGCGCTCCACGCCATCGTTGATTTTTTCAACAGGCATA                                              >  SRR4302545.2489644/1‑94 (MQ=60)
                                             GTAATTAAAGGTGCTTACCATGAGATCCTTTTTGAAAAGGACGCAATGCGCTCAGTCGCGCTCCACGCCATCGTTGATTTTTTCAACAGGCATA                                              >  SRR4302545.526343/1‑94 (MQ=60)
                                             GTAATTAAAGGTGCTTACCATGAGATCCTTTTTGAAAAGGACGCAATGCGCTCAGTCGCGCTCCACGCCATCGTTGATTTTTTCAACAGGCATA                                              >  SRR4302545.8604473/1‑94 (MQ=60)
                                              TAATTAAAGGTGCTTACCATGAGATCCTTTTTGAAAAGGACGCAATGCGCTCAGTCGCGCTCCACGCCATCGTTGATTTTTTCAACAGGCATAA                                             <  SRR4302545.6378723/94‑1 (MQ=60)
                                              TAATTAAAGGGGCTTACCATGAGATCCTTTTTGAAAAGGACGCAATGCTCTCAGTCGCGCTCCACCCCATCGTTGATTTTTTCAACAGGCATAA                                             >  SRR4302545.9276306/1‑94 (MQ=60)
                                              TAATTAAAGGAGCTGACCATGAGATCCTTTTTGGAAAGGACGCAATGCGCTCAGTCGAGCTCCACGCCATCGTTGATTTTTTCAACAGGCATAA                                             >  SRR4302545.10393035/1‑94 (MQ=60)
                                               AATTAAAGGTGCTTACCATGAGATCCTTTTTGAAAAGGACGCAATGCGCTCAGTCGCGCTCCACGCCATCGTTGATTTTTTCAACAGGCATAAC                                            <  SRR4302545.1145832/94‑1 (MQ=60)
                                               AATTAAAGGTGCTTACCATGAGATCCTTTTTGAAAAGGACGCAATGCGCTCAGTCGCGCTCCACGCCATCGTTGATTTTTTCAACAGGCATAAC                                            <  SRR4302545.5019799/94‑1 (MQ=60)
                                               AATTAAAGGTGCTTACCATGAGATCCTTTTTGAAAAGGACGCAATGCGCTCAGTCGCGCTCCACGCCATCGTTGATTTTTTCAACAGGCATAAC                                            >  SRR4302545.9901403/1‑94 (MQ=60)
                                               AATTAAAGGTGCTTACCATGAGATCCTTTTTGAAAAGGACGCAATGCGCTCAGTCGCGCTCAACGCCATCGTTGATTTTTTCAACAGGCATAAC                                            <  SRR4302545.10519753/94‑1 (MQ=60)
                                                ATTAAAGGTGCTTACCATGAGATCCTTTTTGAAAAGGACGCAATGCGCTCAGTCGCGCTCCACGCCATCGTTGATTTTTTCAACAGGCATAACT                                           >  SRR4302545.10791242/1‑94 (MQ=60)
                                                ATTAAAGGTGCTTACCATGAGATCCTTTTTGAAAAGGACGCAATGCGCTCAGTCGCGCTCCACGCCATCGTTGATTTTTTCAACAGGCATAACT                                           <  SRR4302545.855158/94‑1 (MQ=60)
                                                ATTAAAGGTGCTTACCATGAGATCCTTTTTGAAAAGGACGCAATGCGCTCAGTCGCGCTCCACGCCATCGTTGATTTTTTCAACAGGCATAACT                                           >  SRR4302545.4118942/1‑94 (MQ=60)
                                                ATTAAAGGTGCTTACCATGAGATCCTTTTTGAAAAGGACGCAATGCGCTCAGTCGCGCTCCACGCCATCGTTGATTTTTTCAACAGGCATAACT                                           <  SRR4302545.623010/94‑1 (MQ=60)
                                                  TAAAGGTGCTTACCATGAGATCCTTTTTGAAAAGGACGCAATGCGCTCAGTCGCGCTCCACGCCATCGTTGATTTTTTCAACAGGCATAACTCA                                         >  SRR4302545.205050/1‑94 (MQ=60)
                                                  TAAAGGTGCTTACCATGAGATCCTTTTTGAAAAGGACGCAATGCGCTCAGTCGCGCTCCACGCCATCGTTGATTTTTTCAACAGGCATAACTCA                                         <  SRR4302545.3645821/94‑1 (MQ=60)
                                                  TAAAGGTGCTTACCATGAGATCCTTTTTGAAAAGGACGCAATGCGCTCAGTCGCGCTCCACGCCATCGTTGATTTTTTCAACAGGCATAACTCA                                         <  SRR4302545.396467/94‑1 (MQ=60)
                                                  TAAAGGCGCTTACCATGAGATCCTTTTTGAAAAGGACGCAATGCGCTCAGTCGCGCTCCACGCCATCGTTGATTTTTTCAACAGGCATAACTCA                                         >  SRR4302545.10881432/1‑94 (MQ=60)
                                                   AAAGGTGCTTACCATGAGATCCTTTTTGAAAAGGACGCAATGCGCTCAGTCGCGCTCCACGCCATCGTTGATTTTTTCAACAGGCATAACTCAC                                        >  SRR4302545.2193033/1‑94 (MQ=60)
                                                   AAAGGTGCTTACCATGAGATCCTTTTTGAAAAGGACGCAATGCGCTCAGTCGCGCTCCACGCCATCGTTGATTTTTTCAACAGGCATAACTCAC                                        >  SRR4302545.8413749/1‑94 (MQ=60)
                                                    AAGGTGCTTACCATGAGATCCTTTTTGAAAAGGACGCAATGCGCTCAGTCGCGCTCCACGCCATCGTTGATTTTTTCAACAGGCATAACTCACC                                       <  SRR4302545.5769333/94‑1 (MQ=60)
                                                    AAGGTGCTTACCATGAGATCCTTTTTGAAAAGGACGCAATGCGCTCAGTCGCGCTCCACGCCATCGTTGATTTTTTCAACAGGCATAACTCACC                                       >  SRR4302545.6495051/1‑94 (MQ=60)
                                                    AAGGTGCTTACCATGAGATCCTTTTTGAAAAGGACGCAATGCGCTCAGTCGCGCTCCACGCCATCGTTGATTTTTTCAACAGGCATAACTCACC                                       <  SRR4302545.8205068/94‑1 (MQ=60)
                                                    AAGGTGCTTACCATGAGATCCTTTTTGAAAAGGACGCAATGCGCTCAGTCGCGCTCCACGCCATCGTTGATTTTTTCAACAGGCATAACTCACC                                       >  SRR4302545.7052308/1‑94 (MQ=60)
                                                     AGGTGCTTACCATGAGATCCTTTTTGAAAAGGACGCAATGCGCTCAGTCGCGCTCCACGCCATCGTTGATTTTTTCAACAGGCATAACTCACCC                                      >  SRR4302545.414164/1‑94 (MQ=60)
                                                     AGGTGCTTACCATGAGATCCTTTTTGAAAAGGACGCAATGCGCTCAGTCGCGCTCCACGCCATCGTTGATTTTTTCAACAGGCATAACTCACCC                                      >  SRR4302545.3405993/1‑94 (MQ=60)
                                                      agttcgtgCCATGAGATCCTTTTTGAAAAGGACGCAATGCGCTCAGTCGCGCTCCACGCCATCGTTGATTTTTTCAACAGGCATAACTCACCCA                                     <  SRR4302545.3139602/86‑1 (MQ=60)
                                                      GGTGCTTACCATGAGATCCTTTTTGAAAAGGACGCAATGCGCTCAGTCGCGCTCCACGCCATCGTTGATTTTTTCAACAGGCATAACTCACCCA                                     >  SRR4302545.5035129/1‑94 (MQ=60)
                                                      GGTGCTTACCATGAGATCCTTTTTGAAAAGGACGCAATGCGCTCAGTCGCGCTCCACGCCATCGTTGATTTTTTCAACAGGCATAACTCACCCA                                     <  SRR4302545.441012/94‑1 (MQ=60)
                                                      GGTGCTTACCATGAGATCCTTTTTGAAAAGGACGCAATGCGCTCAGTCGCGCTCCACGCCATCGTTGATTTTTTCAACAGGCATAACTCACCCA                                     <  SRR4302545.10037072/94‑1 (MQ=60)
                                                      AGTGCTTACCATGAGATCCTTTTTGAAAAGGACGCAATGCGCTCAGTCGCGCTCCACGCCATCGTTGATTTTTTCAACAGGCATAACTCACCCA                                     <  SRR4302545.3139601/94‑1 (MQ=60)
                                                       GTGCTTACCATGAGATCCTTTTTGAAAAGGACGCAATGCGCTCAGTCGCGCTCCACGCCATCGTTGATTTTTTCAACAGTCATAACTCACCCAG                                    >  SRR4302545.5071403/1‑94 (MQ=60)
                                                        TGCTTACCATGAGATCCTTTTTGAAAAGGACGCAATGCGCTCAGTCGCGCTCCACGCCATCGTTGATTTTTTCAACAGGCATAACTCACCCAGC                                   <  SRR4302545.127773/94‑1 (MQ=60)
                                                        TGCTTACCATGAGATCCTTTTTGAAAAGGACGCAATGCGCTCAGTCGCGCTCCACGCCATCGTTGATTTTTTCAACAGGCATAACTCACCCAGC                                   >  SRR4302545.2698180/1‑94 (MQ=60)
                                                        TGCTTACCATGAGATCCTTTTTGAAAAGGACGCAATGCGCTCAGTCGCGCTCCACGCCATCGTTGATTTTGTCAACAGGCATAACTCACCCAGC                                   <  SRR4302545.6908873/94‑1 (MQ=60)
                                                         GCTTACCATGAGATCCTTTTTGAAAAGGACGCAATGCGCTCAGTCGCGCTCCACGCCATCGTTGATTTTTTCAACAGGCATAACTCACCCAGCG                                  >  SRR4302545.7917340/1‑94 (MQ=60)
                                                         GCTTACCATGAGATCCTTTTTGAAAAGGACGCAATGCGCTCAGTCGCGCTCCACGCCATCGTTGATTTTTTCAACAGGCATAACTCACCCAGCG                                  >  SRR4302545.9934586/1‑94 (MQ=60)
                                                         GCTTACCATGAGATCCTTTTTGAAAAGGACGCAATGCGCTCAGTCGCGCTCCACGCCATCGTTGATTTTTTCAACAGGCATAACTCACCCAGCG                                  <  SRR4302545.1096039/94‑1 (MQ=60)
                                                         GCTTACCATGAGATCCTTTTTGAAAAGGACGCAATGCGCTCAGTCGCGCTCCACGCCATCGTTGATTTTTTCAACAGGCATAACTCACCCAGCG                                  >  SRR4302545.1594213/1‑94 (MQ=60)
                                                          CTTACCATGAGCTCCTTTTTGAAAAGGACGCAATGCGCTCAGTCGCGCTCCACGCCATCGTTGATTTTTTCAACAGGCATAACTCACCCAGCAG                                 >  SRR4302545.6655866/1‑94 (MQ=60)
                                                          CTTACCATGAGATCCTTTTTGAAAAGGACGCAATGCGCTCAGTCGCGCTCCACGCCATCGTTGATTTTTTCAACAGGCATAACTCACCCAGCGG                                 <  SRR4302545.9161523/94‑1 (MQ=60)
                                                           TTACCATGAGATCCTTTTTGAAAAGGACGCAATGCGCTCAGTCGCGCTCCACGCCATCGTTGATTTTTTCAACAGGCATAACTCACCCAGCGGA                                >  SRR4302545.6967296/1‑94 (MQ=60)
                                                           TTACCATGAGATCCTTTTTGAAAAGGACGCAATGCGCTCAGTCGCGCTCCACGCCATCGTTGATTTTTTCAACAGGCATAACTCACCCAGCGGA                                >  SRR4302545.2983698/1‑94 (MQ=60)
                                                            TACCATGAGTTCCTTTTTGAAAAGGACGCAATGCGCTCAGTCGCGCTCCACGCCATCGTTACTTTTTTCAACAGGCATACCTCACCCAGCGGAA                               >  SRR4302545.7425734/1‑94 (MQ=60)
                                                            TACCATGAGATCCTTTTTGAAAAGGACGCAATGCGCTCAGTCGCGCTCCACGCCATCGTTGATTTTTTCAACAGGCATAACTCACCCAGCGGAA                               <  SRR4302545.10360235/94‑1 (MQ=60)
                                                            TACCATGAGATCCTTTTTGAAAAGGACGCAATGCGCTCAGTCGCGCTCCACGCCATCGTTGATTTTTTCAACAGGCATAACTCACCCAGCGGAA                               >  SRR4302545.3494230/1‑94 (MQ=60)
                                                            TACCATGAGATCCTTTTTGAAAAGGACGCAATGCGCTCAGTCGCGCTCCACGCCATCGTTGATTTTTTCAACAGGCATAACTCACCCAGCGGAA                               <  SRR4302545.1388515/94‑1 (MQ=60)
                                                              CCATGAGATCCTTTTTGAAAAGGACGCAATGCGCTCAGTCGCGCTCCACGCCATCGTTGATTTTTTCAACAGGCATAACTCACCCAGCGGAAAC                             >  SRR4302545.10185828/1‑94 (MQ=60)
                                                              CCATGAGATCCTTTTTGAAAAGGACGCAATGCGCTCAGTCGCGCTCCACGCCATCGTTGATTTTTTCAACAGGCATAACTCACCCAGCGGAAAC                             >  SRR4302545.7291772/1‑94 (MQ=60)
                                                               CATGAGATCCTTTTTGAAAAGGACGCAATGCGCTCAGTCGCGCTCCACGCCATCGTTGATTTTTTCAACAGGCATAACTCACCCAGCGGAAACC                            <  SRR4302545.1156616/94‑1 (MQ=60)
                                                                ATGAGATCCTTTTTGAAAAGGACGCAATGCGCTCAGTCGCGCTCCACGCCATCGTTGATTTTTTCAACAGGCATAACTCACCCAGCGGAAACCG                           >  SRR4302545.3713862/1‑94 (MQ=60)
                                                                ATGAGATCCTTTTTGAAAAGGACGCAATGCGCTCAGTCGCGCTCCACGCCATCGTTGATTTTTTCAACAGGCATAACTCACCCAGCGGAAACCG                           >  SRR4302545.1938185/1‑94 (MQ=60)
                                                                ATGAGATCCTTTTTGAAAAGGACGCAATGCGCTCAGTCGCGCTCCACGCCATCGTTGATTTTTTCAACAGGCATAACTCACCCAGCGGAAACCG                           >  SRR4302545.10445585/1‑94 (MQ=60)
                                                                 TGAGATCCTTTTTGAAAAGGACGCAATGCGCTCAGTCGCGCTCCACGCCATCGTTGATTTTTTCAACAGGGATAACTCACCCAGCGGAAACCGC                          >  SRR4302545.7800307/1‑94 (MQ=60)
                                                                 TGAGATCCTTTTTGAAAAGGACGCAATGCGCTCAGTCGCGCTCCACGCCATCGTTGATTTTTTCAACAGGCATAACTCACCCAGCGGAAACCGC                          >  SRR4302545.2366702/1‑94 (MQ=60)
                                                                 TGAGATCCTTTTTGAAAAGGACGCAATGCGCTCAGTCGCGCTCCACGCCATCGTTGATTTTTTCAACAGGCATAACTCACCCAGCGGAAACCGC                          >  SRR4302545.8224216/1‑94 (MQ=60)
                                                                 TGAGATCCTTTTTGAAAAGGACGCAATGCGCTCAGTCGCGCTCCACGCCATCGTTGATTTTTTCAACAGGCATAACTCACCCAGCGGAAACCGC                          >  SRR4302545.5676108/1‑94 (MQ=60)
                                                                 TGAGATCCTTTTTGAAAAGGACGCAATGCGCTCAGTCGCGCTCCACGCCATCGTTGATTTTTTCAACAGGCATAACTCACCCAGCGGAAACCGC                          >  SRR4302545.547700/1‑94 (MQ=60)
                                                                  GAGATCCTTTTTGAAAAGGACGCAATGCGCTCAGTCGCGCTCCACGCCATCGTTGCTTTTTTCAACAGGCATAACTAACCCAGAGGCAAACGCT                         >  SRR4302545.4357795/1‑94 (MQ=60)
                                                                  GAGATCCTTTTTGAAAAGGACGCAATGCGCTCAGTCGCGCTCCACGCCATCGTTGATTTTTTCAACAGGCATAACTCACCCAGCGGAAACCGCT                         >  SRR4302545.4357797/1‑94 (MQ=60)
                                                                   AGATCCTTTTTGAAAAGGACGCAATGCGCTCAGTCGCGCTCCACGCCATCGTTGATTTTTTCAACAGGCTTAACTCACCCAGCGGAAACCGCTC                        <  SRR4302545.3638425/94‑1 (MQ=60)
                                                                   AGATCCTTTTTGAAAAGGACGCAATGCGCTCAGTCGCGCTCCACGCCATCGTTGATTTTTTCAACAGGCATAACTCACCCAGCGGAAACCGCTC                        >  SRR4302545.8649355/1‑94 (MQ=60)
                                                                   AGATCCTTTTTGAAAAGGACGCAATGCGCTCAGTCGCGCTCCACGCCATCGTTGATTTTTTCAACAGGCATAACTCACCCAGCGGAAACCGCTC                        >  SRR4302545.7149497/1‑94 (MQ=60)
                                                                   AGATCCTTTTTGAAAAGGACGCAATGCGCTCAGTCGCGCTCCACGCCATCGTTGATTTTTTCAACAGGCATAACTCACCCAGCGGAAACCGCTC                        >  SRR4302545.7431479/1‑94 (MQ=60)
                                                                   AGATCCTTTTTGAAAAGGACGCAATGCGCTCAGTCGCGCTCCACGCCATCGGTGATTTTTTCAACAGGCATAACTCACCCAGCGGAAAACGCTC                        >  SRR4302545.7085852/1‑94 (MQ=60)
                                                                    GATCCTTTTTGAAAAGGACGCAATGCGCTCAGTCGCGCTCCACGCCATCGTTGATTTTTTCAACAGGCATAACTCACCCAGCGGAAACCGCTCT                       <  SRR4302545.9305285/94‑1 (MQ=60)
                                                                    GATCCTTTTTGAAAAGGACGCAATGCGCTCAGTCGCGCTCCACGCCATCGTTGATTTTTTCAACAGGCATAACTCACCCAGCGGAAACCGCTCT                       <  SRR4302545.10527135/94‑1 (MQ=60)
                                                                    GATCCTTTTTGAAAAGGACGCAATGCGCTCAGTCGCGCTCCACGCCATCGTTGATTTTTTCAACAGGCATAACTCACCCAGCGGAAACCGCTCT                       <  SRR4302545.7852759/94‑1 (MQ=60)
                                                                    GATCCTTTTTGAAAAGGACGCAATGCGCTCAGTCGCGCTCCACGCCATCGTTGATTTTTTCAACAGGCATAACTCACCCAGCGGAAACCGCTCT                       >  SRR4302545.10078840/1‑94 (MQ=60)
                                                                     ATCCTTTTTGAAAAGGACGCAATGCGCTCAGTCGCGCTCCACGCCATCGTTGATTTTTTCAACAGGCATAACTCACCCAGCGGAAACCGCTCTA                      <  SRR4302545.1113693/94‑1 (MQ=60)
                                                                     ATCCTTTTTGAAAAGGACGCAATGCGCTCAGTCGCGCTCCACGCCATCGTTGATTTTTTCAACAGGCATAACTCACCCAGCGGAAACCGCTCTA                      >  SRR4302545.6953925/1‑94 (MQ=60)
                                                                      TCCTTTTTGAAAAGGACGCAATGCGCTCAGTCGCGCTCCACGCCATCGTTGATTTTTTCAACAGGCATAACTCACCCAGCGGAAACCGCTCTAC                     <  SRR4302545.5530506/94‑1 (MQ=60)
                                                                      TCCTTTTTGAAAAGGACGCAATGCGCTCAGTCGCGCTCCACGCCATCGTTGATTTTTTCAACAGGCATAACTCACCCAGCGGAAACCGCTCTAC                     >  SRR4302545.10103602/1‑94 (MQ=60)
                                                                       CCTTTTTGAAAAGGACGCAATGCGCTCAGTCGCGCTCCACGCCATCGTTGATTTTTTCAACAGGCATAACTCACCCAGCGGAAACCGCTCTACA                    <  SRR4302545.5334088/94‑1 (MQ=60)
                                                                       CCTTTTTGAAAAGGACGCAATGCGCTCAGTCGCGCTCCACGCCATCGTTGATTTTTTCAACAGGCATAACTCACCCAGCGGAAACCGCTCTACA                    >  SRR4302545.10651773/1‑94 (MQ=60)
                                                                       CCTTTTTGAAAAGGACGCAATGCGCTCAGTCGCGCTCCACGCCATCGTTGATTTTTTCAACAGGCATAACTCACCCAGCGGAAACCGCTCTACA                    >  SRR4302545.10850230/1‑94 (MQ=60)
                                                                       CCTTTTTGAAAAGGACGCAATGCGCTCAGTCGCGCTCCACGCCATCGTTGATTTTTTCAACAGGCATAACTCACCCAGCGGAAACCGCTCTACA                    >  SRR4302545.1042253/1‑94 (MQ=60)
                                                                        CTTTTTGAAAAGGACGCAATGCGCTCAGTCGCGCTCCACGCCATCGTTGATTTTTTCAACAGGCATAACTCACCCAGCGGAAACCGCTCTACAG                   >  SRR4302545.4218239/1‑94 (MQ=60)
                                                                        CTTTTTGAAAAGGACGCAATGCGCTCAGTCGCGCTCCACGCCATCGTTGATTTTTTCAACAGGCATAACTCACCCAGCGGAAACCGCTCTACAG                   >  SRR4302545.10581864/1‑94 (MQ=60)
                                                                         TTTTTGAAAAGGACGCAATGCGCTCAGTCGCGCTCCACGCCATCGTTGATTTTTTCAACAGGCATAACTCACCCAGCGGAAACCGCTCTACAGA                  >  SRR4302545.5646541/1‑94 (MQ=60)
                                                                         TTTTTGAAAAGGACGCAATGCGCTCAGTCGCGCTCCACGCCATCGTTGATTTTTTCAACAGGCATAACTCACCCAGCGGAAACCGCTCTACAGA                  <  SRR4302545.10608701/94‑1 (MQ=60)
                                                                         TTTTTGAAAAGGACGCAATGCGCTCAGTCGCGCTCCACGCCATCGTTGATTTTTTCAACAGGCATAACTCACCCAGCGGAAACCGCTCTACAGA                  >  SRR4302545.5761449/1‑94 (MQ=60)
                                                                         TTTTTGAAAAGGACGCAATGCGCTCAGTCGCGCTCCACGCCATCGTTGATTTTTTCAACAGGCATAACTCACCCAGCGGAAACCGCTCTACAGA                  >  SRR4302545.6156355/1‑94 (MQ=60)
                                                                          TTTTGAAAAGGACGCAATGCGCTCAGTCGCGCTCCACGCCATCGTTGATTTTTTCAACAGGCATAACTCACCCAGCGGAAACCGCTCTACAGAG                 <  SRR4302545.2841639/94‑1 (MQ=60)
                                                                          TTTTGAAAAGGACGCAATGCGCTCAGTCGCGCTCCACGCCATCGTTGATTTTTTCAACAGGCATAACTCACCCAGCGGAAACCGCTCTACAGAG                 <  SRR4302545.2867935/94‑1 (MQ=60)
                                                                          TTTTGAAAAGGACGCAATGCGCTCAGTCGCGCTCCACGCCATCGTTGATTTTTTCAACAGGCATAACTCACCCAGCGGAAACCGCTCTACAGAG                 >  SRR4302545.8430190/1‑94 (MQ=60)
                                                                          TTTTGAAAAGGACGCAATGCGCTCAGTCGCGCTCCACGCCATCGTTGATTTTTTCAACAGGCATAACTCACCCAGCGGAAACCGCTCTACAGAG                 <  SRR4302545.3932526/94‑1 (MQ=60)
                                                                             TGAAAAGGACGCAATGCGCTCAGTCGCGCTCCACGCCATCGTTGATTTTTTCAACAGGCATAACTCACCCAGCGGAAACCGCTCTACAGAGGTT              >  SRR4302545.3274209/1‑94 (MQ=60)
                                                                             TGAAAAGGACGCAATGCGCTCAGTCGCGCTCCACGCCATCGTTGATTTTTTCAACAGGCATAACTCACCCAGCGGAAACCGCTCTACAGAGGTT              >  SRR4302545.6504649/1‑94 (MQ=60)
                                                                              GAAAAGGACGCAATGCGCTCAGTCGCGCTCCACGCCATCGTTGATTTTTTCAACAGGCATAACTCACCCAGCGGAAACCGCTCTACAGAGGTTT             >  SRR4302545.7839341/1‑94 (MQ=60)
                                                                                AAAGGACGCAATGCGCTCAGTCGCGCTCCACGCCATCGTTGATTTTTTCAACAGGCATAACTCACCCAGCGGAAACCGCTCTACAGAGGTTTAA           <  SRR4302545.8712168/94‑1 (MQ=60)
                                                                                AAAGGACGCAATGCGCTCAGTCGCGCTCCACGCCATCGTTGATTTTTTCAACAGGCATAACTCACCCAGCGGAAACCGCTCTACAGAGGTTTAA           >  SRR4302545.10056766/1‑94 (MQ=60)
                                                                                AAAGGACGCAATGCGCTCAGTCGCGCTCCACGCCATCGTTGATTTTTTCAACAGGCATAACTCACCCAGCGGAAACCGCTCTACAGAGGGTTAA           >  SRR4302545.7813266/1‑94 (MQ=60)
                                                                                 AAGGACGCAATGCGCTCAGTCGCGCTCCACGCCATCGTTGATTTTTTCAACAGGCATAACTCACCCAGCGGAAACCGCTCTACAGAGGTTTAAA          >  SRR4302545.2130322/1‑94 (MQ=60)
                                                                                  AGGACGCAATGCGCTCAGTCGCGCTCCACGCCATCGTTGATTTTTTCAACAGGCATAACTCACCCAGCGGAAACCGCTCTACAGAGGTTTAAAT         <  SRR4302545.10552843/94‑1 (MQ=60)
                                                                                  AGGACGCAATGCGCTCAGTCGCGCTCCACGCCATCGTTGATTTTTTCAACAGGCATAACTCACCCAGCGGAAACCGCTCTACAGAGGTTTAAAT         >  SRR4302545.8213112/1‑94 (MQ=60)
                                                                                  AGGACGCAATGCGCTCAGTCGCGCTCCACGCCATCGTTGATTTTTTCAACAGGCATAACTCACCCAGCGGAAACCGCTCTACAGAGGTTTAAAT         >  SRR4302545.2147323/1‑94 (MQ=60)
                                                                                  AGGACGCAATGCGCTCAGTCGCGCTCCACGCCATCGTTGATTTTTTCAACAGGCATAACTCACCCAGCGGAAACCGCTCTACAGAGGTTTAAAT         >  SRR4302545.3977329/1‑94 (MQ=60)
                                                                                   GGACGCAATGCGCTCAGTCGCGCTCCACGCCATCGTTGATTTTTTCAACAGGCATAACTCACCCAGCGGAAACCGCTCTACAGAGGTTTAAATT        >  SRR4302545.1365949/1‑94 (MQ=60)
                                                                                    GACGCAATGCGCTCAGTCGCGCTCCACGCCATCGTTGATTTTTTCAACAGGCATAACTCACCCAGCGGAAAACGCTCTACAGAGGTTTAAATTT       <  SRR4302545.10708945/94‑1 (MQ=60)
                                                                                     ACGCAATGCGCTCAGTCGCGCTCCACGCCATCGTTGATTTTTTCAACAGGCATAACTCACCCAGCGGAAACCGCTCTACAGAGGTTTAAATTTC      >  SRR4302545.927486/1‑94 (MQ=60)
                                                                                      CGCAATGCGCTCAGTCGCGCTCCACGCCATCGTTGATTTTTTCAACAGGCATAACTCACCCAGCGGAAACCGCTCTACAGAGGTTTAAATTTCT     <  SRR4302545.5142483/94‑1 (MQ=60)
                                                                                      CGCAATGCGCTCAGTCGCGCTCCACGCCATCGTTGATTTTTTCAACAGGCATAACTCACCCAGCGGAAACCGCTCTACAGAGGTTTAAATTTCT     <  SRR4302545.8884932/94‑1 (MQ=60)
                                                                                      CGCAATGCGCTCAGTCGCGCTCCACGCCATCGTTGATTTTTTCAACAGGCATAACTCACCCAGCGGAAACCGCTCTACAGAGGTTTAAATTTCT     >  SRR4302545.1628600/1‑94 (MQ=60)
                                                                                       GCAATGCGCTCAGTCGCGCTCCACGCCATCGTTGATTTTTTCAACAGGCATAACTCACCCAGCGGAAACCGCTCTACAGAGGTTTAAATTTCTT    >  SRR4302545.753856/1‑94 (MQ=60)
                                                                                        CAATGCGCTCAGTCGCGCTCCACGCCATCGTTGATTTTTTCAACAGGCATAACTCACCCAGCGGAAACCGCTCTACAGAGGTTTAAATTTCTTA   <  SRR4302545.10899841/94‑1 (MQ=60)
                                                                                        CAATGCGCTCAGTCGCGCTCCACGCCATCGTTGATTTTTTCAACAGGCATAACTCACCCAGCGGAAACCGCTCTACAGAGGTTTAAATTTCTTA   <  SRR4302545.5092950/94‑1 (MQ=60)
                                                                                        CAATGCGCTCAGTCGCGCTCCACGCCATCGTTGATTTTTTCAACAGGCATAACTCACCCAGCGGAAACCGCTCTACAGAGGTTTAAATTTCTTA   >  SRR4302545.2347609/1‑94 (MQ=60)
                                                                                        CAATGCGCTCAGTCGCGCTCCACGCCATCGTTGATTTTTTCAACAGGCATAACTCACCCAGCGGAAACCGCTCTACAGAGGTTTAAATTTCTTA   >  SRR4302545.673702/1‑94 (MQ=60)
                                                                                         AATGCGCTCAGTCGCGCTCCACGCCATCGTTGATTTTTTCAACAGGCATAACTCACCCAGCGGAAACCGCTCTACAGAGGTTTAAATTTCTTAT  >  SRR4302545.6305676/1‑94 (MQ=60)
                                                                                         AATGCGCTCAGTCGCGCTCCACGCCATCGTTGATTTTTTCAACAGGCATAACTCACCCAGCGGAAACCGCTCTACAGAGGTTTAAATTTCTTAT  >  SRR4302545.1179589/1‑94 (MQ=60)
                                                                                         AATGCGCTCAGTCGCGCTCCACGCCATCGTTGATTTTTTCAACAGGCATAACTCACCCAGCGGAAACCGCTCTACAGAGGTTTAAATTTCTTAT  >  SRR4302545.5116836/1‑94 (MQ=60)
                                                                                         AATGCGCTCAGTCGCGCTCCACGCCATCGTTGATTTTTTCAACAGGCATAACTCACCCAGCGGAAACCGCTCTACAGAGGTTTAAATTTCTTAT  >  SRR4302545.256/1‑94 (MQ=60)
                                                                                         AATGCGCTCAGTCGCGCTCCACGCCATCGTTGATTTTTTCAACAGGCATAACTCACCCAGCGGAAACCGCTCTACAGAGGTTTAAATTTCTTAT  >  SRR4302545.3555701/1‑94 (MQ=60)
                                                                                             |                                                                                         
CTCCGCACCGCCGCGGGCCATCCTGTCGAAGGAGGACGGCCGTTGGTAATTAAAGGTGCTTACCATGAGATCCTTTTTGAAAAGGACGCAATGGCCTCAGTCGCGCTCCACGCCATCGTTGATTTTTTCAACAGGCATAACTCACCCAGCGGAAACCGCTCTACAGAGGTTTAAATTTCTTAT  >  NC_000913/4007625‑4007807

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 7 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑