Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I1 R1 647 270.1 21258240 98.4% 20918108 94.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 4,008,421 +T coding (504/516 nt) b4555 → hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,008,4211.T99.7% 1143.5 / ‑4.3 301coding (504/516 nt)b4555hypothetical protein
Reads supporting (aligned to +/- strand):  ref base . (1/0);  new base T (151/149);  total (152/149)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.99e-01

Only 100 of 307 total aligned reads displayed.

GTCTGGAAGTGATGGCGGGCGGCGTTTC‑AAAGGCCATGCGCTGGAAG‑GCTGGCG‑‑‑‑AA‑‑‑‑CT‑GGCTACAG‑CTGAAGGATT‑G‑ATTGCGTT‑‑GTGACGGGATGAACGACGCCGAAATGCTGTCGATGGC‑GGGAAA‑GCTGCATTATGGGCAGTGGCCACCAGCGTCTG  >  NC_000913/4008345‑4008504
                                                                                          |                                                                                       
gTCTGGAAGTGATGGCGGGCGGCGTTTCAAAAGGCCATGCGCTGGAAGCGGTGGCGAAGAAA‑‑‑‑CTGGGCTACAGCCTGAAGGATT‑GTATTGCGtt                                                                                 >  1:7797595/1‑94 (MQ=255)
gTCTGGAAGTGATGGCGGGCGGCGTTTCAAAAGGCCATGCGCTGGAAGCGGTGGCG‑‑‑‑AAGAATCTGGGCTACAGCCTGAAGGATT‑GTATTGCGtt                                                                                 <  1:502475/94‑1 (MQ=255)
  cTGGAAGTGATGGCGGGCGGCGTTTCAAAAGGCCATGCGCTGGAAGCGGTGGCGAAGAAA‑‑‑‑CTGGGCTACAGCCTGAAGGATT‑GTATTGCGtttg                                                                               <  1:8584841/94‑3 (MQ=255)
  cTGGAAGTGATGGCGGGCGGCGTTTCAAAAGGCCATGCGCTGGAAGCGGTGGCGAAGAAA‑‑‑‑CTGGGCTACAGCCTGAAGGATT‑GTATTGCGtttg                                                                               >  1:7683641/1‑92 (MQ=255)
  cTGGAAGTGATGGCGGGCGGCGTTTCAAAAGGCCATGCGCTGGAAGCGGTGGCGAAGAAA‑‑‑‑CTGGGCTACAGCCTGAAGGATT‑GTATTGCGtttg                                                                               <  2:7792987/94‑3 (MQ=255)
   tGGAAGTGATGGCGGGCGGCGTTTCAAAAGGCCATGCGCTGGAAGCGGTGGCGAAGAAA‑‑‑‑CTGGGCTACAGCCTGAAGGATT‑GTATTGCGtttgg                                                                              >  1:4852832/1‑91 (MQ=255)
   tGGAAGTGATGGCGGGCGGCGTTTCAAAAGGCCATGCGCTGGAAGCGGTGGCGAAGAAA‑‑‑‑CTGGGCTACAGCCTGAAGGATT‑GTATTGCGtttgg                                                                              >  1:8323723/1‑91 (MQ=255)
    ggAAGTGATGGCGGTCGGCGTTTCAAAAGGCCATGCGCTGGAAGCGGTGGCGAAGAAA‑‑‑‑CTGGGCTACAGCCTGAAGGATT‑GTATTGCGtttggt                                                                             <  2:3969802/94‑5 (MQ=255)
          gATGGCGGGCGGCGTTTCAAAAGGCCATGCGCTGGAAGCGGTGGCGAAGAAA‑‑‑‑CTGGGCTACAGCCTGAAGGATT‑GTATTGCGTTTGGTGACggg                                                                       <  1:1219882/94‑1 (MQ=255)
          gATGGCGGGCGGCGTTTCAAAAGGCCATGCGCTGGAAGCGGTGGCGAAGAAA‑‑‑‑CTGGGCTACAGCCTGAAGGATT‑GTATTGCGTTTGGTGACggg                                                                       >  1:10380994/1‑94 (MQ=255)
          gATGGCGGGCGGCGTTTCAAAAGGCCATGCGCTGGAAGCGGTGGCGAAGAAA‑‑‑‑CTGGGCTACAGCCTGAAGGATT‑GTATTGCGTTTGGTGACggg                                                                       >  1:7931245/1‑94 (MQ=255)
          gATGGCGGGCGGCGTTTCAAAAGGCCATGCGCTGGAAGCGGTGGCGAAGAAA‑‑‑‑CTGGGCTACAGCCTGAAGGATT‑GTATTGCGTTTGGTGACggg                                                                       <  1:10280941/94‑1 (MQ=255)
          gATGGCGGGCGGCGTTTCAAAAGGCCATGCGCTGGAAGCGGTGGCGAAGAAA‑‑‑‑CTGGGCTACAGACTGAAGGATT‑GTATTGCGTTTGGTGACggg                                                                       >  2:4202765/1‑94 (MQ=255)
            tGGCGGGCGGCGTTTCAAAAGGCCATGCGCTGGAAGCGGTGGCGAAGAAA‑‑‑‑CTGGGCTACAGCCTGAAGGATT‑GTATTGCGTTTGGTGACGGGag                                                                     >  2:716483/1‑93 (MQ=255)
             ggcgggcgGCGTTTCAAAAGGCCATGCGCTGGAAGCGGTGGCGAAGAAA‑‑‑‑CTGGGCTACAGCCTGAAGGATT‑GTATTGCGTTTGGTGACGGGATg                                                                    >  1:1980290/1‑94 (MQ=255)
             ggcgggcgGCGTTTCAAAAGGCCATGCGCTGGAAGCGGTGGCGAAGAAA‑‑‑‑CTGGGCTACAGCCTGAAGGATT‑GTATTGCGTTTGGTGACGGGATg                                                                    <  2:6192011/94‑1 (MQ=255)
              gcgggcggCGTTTCAAAAGGCCATGCGCTGGAAGCGGTGGCGAAGAAA‑‑‑‑CTGGGCTACAGCCTGAAGGATT‑GTATTGCGTTTGGTGACGGGATGa                                                                   <  2:4537753/94‑1 (MQ=255)
               cgggcggCGTTTCAAAAGGCCATGCGCTGGAAGCGGTGGCGAAGAAA‑‑‑‑CTGGGCTACAGCCTGAAGGATT‑GTATTGCGTTTGGTGACGGGATGaa                                                                  <  2:2803961/94‑1 (MQ=255)
               cgggcggCGTTTCAAAAGGCCATGCGCTGGAAGCGGTGGCGAAGAAA‑‑‑‑CTGGGCTACAGCCTGAAGGATT‑GTATTGCGTTTGGTGACGGGATGaa                                                                  >  2:2262793/1‑94 (MQ=255)
               cgggcggCGTTTCAAAAGGCCATGCGCTGGAAGCGGTGGCGAAGAAA‑‑‑‑CTGGGCTACAGCCTGAAGGATT‑GTATTGCGTTTGGTGACGGGATGaa                                                                  <  2:4615906/94‑1 (MQ=255)
               cgggcggCGTTTCAAAAGGCCATGCGCTGGAAGCGGTGGCGAAGAAA‑‑‑‑CTGGGCTACAGCCTGAAGGATT‑GTATTGCGTTTGGTGACGGGATGaa                                                                  >  2:8736980/1‑94 (MQ=255)
               cgggcggCGTTTCAAAAGGCCATGCGCTGGAAGCGGTGGCGAAGAAA‑‑‑‑CTGGGCTACAGCCTGAAGGATT‑GTATTGCGTTTGGTGACGGGATGaa                                                                  <  2:9856631/94‑1 (MQ=255)
               cgggcggCGTTTCAAAAGGCCATGCGCTGGAAGCGGTGGCGAAGAAA‑‑‑‑CTGGGCTACAGCCTGAAGGATT‑GTATTGCGTTTGGTGACGGGATGaa                                                                  >  1:3470749/1‑94 (MQ=255)
                gggcggCGTTTCAAAAGGCCATGCGCTGGAAGCGGTGGCGAAGAAA‑‑‑‑CTGGGCTACAGCCTGAAGGATT‑GTATTGCGTTTGGTGACGGGATGaag                                                                 >  2:1684731/1‑93 (MQ=255)
                gggcggCGTTTCAAAAGGCCATGCGCTGGAAGCGGTGGCGAAGAAA‑‑‑‑CTGGGCTACAGCCTGAAGGATT‑GTATTGCGTTTGGTGACGGGATGAac                                                                 <  2:6050420/94‑1 (MQ=255)
                gggcggCGTTTCAAAAGGCCATGCGCTGGAAGCGGTGGCGAAGAAA‑‑‑‑CTGGGCTACAGCCTGAAGGATT‑GTATTGCGTTTGGTGACGGGATGAac                                                                 <  2:10164761/94‑1 (MQ=255)
                gggcggCGTTTCAAAAGGCCATGCGCTGGAAGCGGTGGCGAAGAAA‑‑‑‑CTGGGCTACAGCCTGAAGGATT‑GTATTGCGTTTGGTGACGGGATGAac                                                                 <  2:1996423/94‑1 (MQ=255)
                gggcggCGTTTCAAAAGGCCATGCGCTGGAAGCGGTGGCGAAGAAA‑‑‑‑CTGGGCTACAGCCTGAAGGATT‑GTATTGCGTTTGGTGACGGGATGAac                                                                 <  2:5461396/94‑1 (MQ=255)
                  gcggcgTTTCAAAAGGCCATGCGCTGGAAGCGGTGGCGAAGAAA‑‑‑‑CTGGGCTAGAGCCTGAAGGATT‑GTATTGCGTTTGGTGCCGGGATGAacga                                                               >  2:6573984/1‑94 (MQ=255)
                  gcggcgTTTCAAAAGGCCATGCGCTGGAAGCGGTGGCGAAGAAA‑‑‑‑CTGGGCTACAGCCTGAAGGATT‑GTATTGCGTTTGGTGACGGGATGAacga                                                               <  1:5201171/94‑1 (MQ=255)
                  gcggcgTTTCAAAAGGCCATGCGCTGGAAGCGGTGGCGAAGAAA‑‑‑‑CTGGGCTACAGCCTGAAGGATT‑GTATTGCGTTTGGTGACGGGATGAacga                                                               <  1:9298965/94‑1 (MQ=255)
                  gccgcgTTTCAAAAGGCCATGCGCTGGAAGCGGTGGCGAAGAAA‑‑‑‑CTGGGCTACAGCCTGAAGGATT‑GTATTGCGTTTGGTGACGGGATGAacga                                                               <  1:4802832/91‑1 (MQ=255)
                   cggcgTTTCAAAAGGCCATGCGCTGGAAGCGGTGGCGCAGAAA‑‑‑‑CTGGGCTACAGCCTGAAGGATT‑GTATTGCGTTTGGTGACGGGATGAacgac                                                              >  2:492205/1‑94 (MQ=255)
                   cggcgTTTCAAAAGGCCATGCGCTGGAAGCGGTGGCGAAGAAA‑‑‑‑CTGGGCTACATCCTGAAGGATT‑GTATTGCGTTTGGTGACGGGATGAacgac                                                              <  2:5669141/94‑1 (MQ=255)
                   cggcgTTTCAAAAGGCCATGCGCTGGAAGCGGTGGCGAAGAAA‑‑‑‑CTGGGCTACAGCCTGAAGGATT‑GTATTGCGTTTGGTGACGGGATGAacgac                                                              >  2:8629714/1‑94 (MQ=255)
                   cggcgTTTCAAAAGGCCATGCGCTGGAAGCGGTGGCGAAGAAA‑‑‑‑CTGGGCTACAGCCTGAAGGATT‑GTATTGCGTTTGGTGACGGGATGAacgac                                                              >  1:4446341/1‑94 (MQ=255)
                   cggcgTTTCAAAAGGCCATGCGCTGGAAGCGGTGGCGAAGAAA‑‑‑‑CTGGGCTACAGCCTGAAGGATT‑GTATTGCGTTTGGTGACGGGATGAacgac                                                              <  2:4749689/94‑1 (MQ=255)
                   cggcgTTTCAAAAGGCCATGCGCTGGAAGCGGTGGCGAAGAAA‑‑‑‑CTGGGCTACAGCCTGAAGGATT‑GTATTGCGTTTGGTGACGGGATGAacgac                                                              <  1:8710398/94‑1 (MQ=255)
                   cggcgTTTCAAAAGGCCATGCGCTGGAAGCGGTGGCGAAGAAA‑‑‑‑CTGGGCTACAGCCTGAAGGATT‑GTATTGCGTTTGGTGACGGGATGAacgac                                                              <  1:9738226/94‑1 (MQ=255)
                    ggcgTTTCAAAAGGCCATGCGCTGGAAGCGGTGGCGAAGAAA‑‑‑‑CTGGGCTACAGCCTGAAGGATT‑GTATTGCGTTTGGTGACGGGATGAacgacg                                                             <  2:745645/94‑1 (MQ=255)
                    ggcgTTTCAAAAGGCCATGCGCTGGAAGCGGTGGCGAAGAAA‑‑‑‑CTGGGCTACAGCCTGAAGGATT‑GTATTGCGTTTGGTGACGGGATGAacgacg                                                             >  1:4606483/1‑94 (MQ=255)
                    ggcgTTTCAAAAGGCCATGCGCTGGAAGCGGTGGCGAAGAAA‑‑‑‑CTGGGCTACAGCCTGAAGGATT‑GTATTGCGTTTGGTGACGGGATGAacgacg                                                             >  1:10216837/1‑94 (MQ=255)
                     gcgTTTCAAAAGGCCATGCGCTGGAAGCGGTGGCGAAGAAA‑‑‑‑CTGGGCTACAGCCTGAAGGATT‑GTATTGCGTTTGGTGACGGGATGAACGACGc                                                            >  1:1848845/1‑94 (MQ=255)
                      cgTTTCAAAAGGCCATGCGCTGGAAGCGGTGGCGAAGAAA‑‑‑‑CTGGGCTACAGCCTGAAGGATT‑GTATTGCGTTTGGTGACGGGATGAACGACGcc                                                           <  2:4805074/94‑1 (MQ=255)
                      cgTTTCAAAAGGCCATGCGCTGGAAGCGGTGGCGAAGAAA‑‑‑‑CTGGGCTACAGCCTGAAGGATT‑GTATTGCGTTTGGTGACGGGATGAACGACGcc                                                           >  2:9201656/1‑94 (MQ=255)
                      cgTTTCAAAAGGCCATGCGCTGGAAGCGGTGGCGAAGAAA‑‑‑‑CTGGGCTACAGCCTGAAGGATT‑GTATTGCGTTTGGTGACGGGATGAACGACGcc                                                           >  1:6085888/1‑94 (MQ=255)
                       gTTTCAAAAGGCCATGCGCTGGAAGCGGTGGCGAAGAAA‑‑‑‑CTGGGCTACAGCCTGAAGGATTGG‑ATTGCGTTTGGTGACGGGATGAACGACCCCg                                                          >  2:4874565/1‑94 (MQ=255)
                       gTTTCAAAAGGCCATGCGCTGGAAGCGGTGGCGAAGAAA‑‑‑‑CTGGGCTACAGCCTGAAGGATT‑GTATTGCGTTTGGTGACGGGATGAACGACGCCg                                                          >  2:3693511/1‑94 (MQ=255)
                       gTTTCAAAAGGCCATGCGCTGGAAGCGGTGGCGAAGAAA‑‑‑‑CTGGGCTACAGCCTGAAGGATT‑GTATTGCGTTTGGTGACGGGATGAACGACGCCg                                                          >  2:5958902/1‑94 (MQ=255)
                           caaaaGGCCATGCGCTGGAAGCGGTGGCGAAGAAA‑‑‑‑CTGGGCTACAGCCTGAAGGATT‑GTATTGCGTTTGGTGACGGGATGAACGACGCCGAAAt                                                      <  1:8241931/92‑1 (MQ=255)
                           caaaaGGCCATGCGCTGGAAGCGGTGGCGAAGAAA‑‑‑‑CTGGGCTACAGCCTGAAGGATT‑GTATTGCGTTTGGTGACGGGATGAACGACGCCGAAAt                                                      <  2:8968878/92‑1 (MQ=255)
                           caaaaGGCCATGCGCTGGAAGCGGTGGCGAAGAAA‑‑‑‑CTGGGCTACAGCCTGAAGGATT‑GTATTGCGTTTGGTGACGGGATGAACGACGCCGAAAt                                                      >  1:2298838/3‑94 (MQ=255)
                           caaaaGGCCATGCGCTGGAAGCGGTGGCGAAGAAA‑‑‑‑CTGGGCTACAGCCTGAAGGATT‑GTATTGCGTTTGGTGACGGGATGAACGACGCCGAAAt                                                      <  2:58098/92‑1 (MQ=255)
                            aaaaGGCCATGCGCTGGAAGCGGTGGCGAAGAAA‑‑‑‑CTGGGCTACAGCCTGAAGGATT‑GTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATg                                                     <  2:705039/93‑1 (MQ=255)
                            aaaaGGCCATGCGCTGGAAGCGGTGGCGAAGAAA‑‑‑‑CTGGGCTACAGCCTGAAGGATT‑GTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATg                                                     >  1:306375/2‑94 (MQ=255)
                             aaaGGCCATGCGCTGGAAGCGGTGGCGAAGAAA‑‑‑‑CTGGGCTACAGCCTGAAGGATT‑GTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGc                                                    <  1:1012806/94‑1 (MQ=255)
                               aGGCCATGCGCTGGAAGCGGTGGCGAAGAAA‑‑‑‑CTGGGCTACAGCCTGAAGGATT‑GTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTg                                                  >  2:295532/1‑94 (MQ=255)
                               aGGCCATGCGCTGGAAGCGGTGGCGAAGAAA‑‑‑‑CTGGGCTACAGCCTGAAGGATT‑GTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTg                                                  <  2:2778905/94‑1 (MQ=255)
                                ggCCATGCGCTGGAAGCGGTGGCGAAGAAA‑‑‑‑CTGGGCTACAGCCTGAAGGATT‑GTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGt                                                 <  2:3587287/94‑1 (MQ=255)
                                ggCCATGCGCTGGAAGCGGTGGCGAAGAAA‑‑‑‑CTGGGCTACAGCCTGAAGGATT‑GTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGt                                                 <  1:6686780/94‑1 (MQ=255)
                                                                 actgggCTACAGCCTGAAGGATT‑GTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGCGGGGAAAGGCTGCATTATGGGc                    <  1:6282792/90‑1 (MQ=255)
                                                                  ctgggCTACAGCCTGAAGGATT‑GTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGCGGGGAAAGGCTGCATTATGGGcg                   >  2:8095172/4‑93 (MQ=255)
                                                                  ctgggCTACAGCCTGAAGGATT‑GTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGCGGGGAAAGGCTGCATTATGGGCa                   >  2:4390419/4‑94 (MQ=255)
                                                                  ctgggCTACAGCCTGAAGGATT‑GTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGCGGGGAAAGGCTGCATTATGGGCa                   <  2:4995888/91‑1 (MQ=255)
                                                                  ctgggCTACAGCCTGAAGGATT‑GTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGCGGGGAAAGGCTGCATTATGGGCa                   <  1:3513802/91‑1 (MQ=255)
                                                                  ctgggCTACAGCCTGAAGGATT‑GTATTGCGTTTGGCGACGGGATGAACGAAGCCGAAATGCTGTCGATGGCGGGGAAAGGCTGCATTATTGGcg                   >  1:4539305/4‑93 (MQ=255)
                                                                   tgggCTACAGCCTGAAGGATT‑GTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGCGGGGAAAGGCTGCATTATGGGCAg                  <  2:5158518/92‑1 (MQ=255)
                                                                    gggCTACAGCCTGAAGGATT‑GTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGCGGGGAAAGGCTGCATTATGGGCAGt                 <  2:10542244/93‑1 (MQ=255)
                                                                     ggCTACAGCCTGAAGGATT‑GTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGCGGGGAAAGGCTGCATTATGGGCAGTg                <  1:1469160/94‑1 (MQ=255)
                                                                     ggCTACAGCCTGAAGGATT‑GTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGCGGGGAAAGGCTGCATTATGGGCAGTg                <  2:8197731/94‑1 (MQ=255)
                                                                     ggCTACAGCCTGAAGGATT‑GTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGCGGGGAAAGGCTGCATTATGGGCAGTg                >  2:3796633/1‑94 (MQ=255)
                                                                       cTACAGCCTGAAGGATT‑GTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGCGGGGAAAGGCTGCATTATGGGCAGTgcg              <  1:4748663/94‑3 (MQ=255)
                                                                       cTACAGCCTGAAGGATT‑GTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGCGGGGAAAGGCTGCATTATGGGCAGTgcg              >  1:5564699/1‑92 (MQ=255)
                                                                       cTACAGCCTGAAGGATT‑GTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGCGGGGAAAGGCTGCATTATGGGCAGTgcg              >  2:6649350/1‑92 (MQ=255)
                                                                           agccTGAAGGATT‑GTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGCGGGGAAAGGCTGCATTATGGGCAGTGCgcacc          <  1:2651406/91‑1 (MQ=255)
                                                                           agccTGAAGGATT‑GTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGCGGGGAAAGGCTGCATTATGGGCAGTGCgcacc          >  1:279937/4‑94 (MQ=255)
                                                                           agccTGAAGGATT‑GTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGCGGGGAAAGGCTGCATTATGGGCAGTGCgcacc          >  2:8473482/4‑94 (MQ=255)
                                                                            gccTGAAGGATT‑GTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGCGGGGAAAGGCTGCATTATGGGCAGTGCgcacca         >  1:751673/3‑94 (MQ=255)
                                                                             ccTGAAGGATT‑GTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGCGGGGAAAGGCTGCATTATGGGCAGTGCGCACCAg        <  2:9083253/93‑1 (MQ=255)
                                                                             ccTGAAGGATT‑GTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGCGGGGAAAGGCTGCATTATGGGCAGTGCGCACCAg        <  2:6471042/93‑1 (MQ=255)
                                                                             ccTGAAGGATT‑GTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGCGGGGAAAGGCTGCATTATGGGCAGTGCGCACCAg        >  2:1230917/2‑94 (MQ=255)
                                                                              cTGAAGGATT‑GTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGCGGGGAAATGCTGCATTATGGGCAGTGCGCACCAGc       >  2:4138522/1‑94 (MQ=255)
                                                                              cTGAAGGATT‑GTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGCGGGGAAAGGCTGCATTATGGGCAGTGCGCACCAGc       <  1:541609/94‑1 (MQ=255)
                                                                              cTGAAGGATT‑GTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGCGGGGAAAGGCTGCATTATGGGCAGTGCGCACCAGc       <  2:2318720/94‑1 (MQ=255)
                                                                               tGAAGGATT‑GTATTGGGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGCGGGGAAAGGCTGCATTATGGGCAGTGCGCACCAGCg      <  2:1811505/94‑1 (MQ=255)
                                                                               tGAAGGATT‑GTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGCGGGGAAATGCTGCATTATGGGCAGTGAGCACCAGCg      >  2:2526998/1‑94 (MQ=255)
                                                                               tGAAGGATT‑GTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGCGGGGAAAGGCTGCATTATGGGCAGTGCGCACCAGCg      <  1:3945179/94‑1 (MQ=255)
                                                                               tGAAGGATT‑GTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGCGGGGAAAGGCTGCATTATGGGCAGTGCGCACCAGCg      >  2:2754678/1‑94 (MQ=255)
                                                                                gAAGGATT‑GTATTGCGTTTGGTGACGGGATGAACGTCGCCGAAATGCTGTCGATGGCGGGGAAAGGCTGCATTATGGGCAGTGCGCACCAGCGt     <  1:2599168/94‑1 (MQ=255)
                                                                                gAAGGATT‑GTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGCGGGGAAAGGCTGCATTATGGGCAGTGGGCACCAGCGt     >  2:10092496/1‑94 (MQ=255)
                                                                                gAAGGATT‑GTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGCGGGGAAAGGCTGCATTATGGGCAGTGCGCACCAGCGt     >  2:7115657/1‑94 (MQ=255)
                                                                                gAAGGATT‑GTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGCGGGGAAAGGCTGCATTATGGGCAGTGCGCACCAGCGt     <  2:8243046/94‑1 (MQ=255)
                                                                                 aaGGATT‑GTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGCGGGGAAAGGCTGCATTATGGGCAGTGCGCACCAGCGTc    <  2:1363685/94‑1 (MQ=255)
                                                                                 aaGGATT‑GTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGCGGGGAAAGGCTGCATTATGGGCAGTGCGCACCAGCGTc    <  1:6985014/94‑1 (MQ=255)
                                                                                 aaGGATT‑GTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGCGGGGAAAGGCTGCATTATGGGCAGTGCGCACCAGCGTc    >  1:10548514/1‑94 (MQ=255)
                                                                                  aGGATT‑GTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGCGGGGAAAGGCTGCATTATGGGCAGTGCGCACCAGCGTCt   >  2:6968644/1‑94 (MQ=255)
                                                                                  aGGATT‑GTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGCGGGGAAAGGCTGCATTATGGGCAGTGCGCACCAGCGTCt   >  2:2371359/1‑94 (MQ=255)
                                                                                  aGGATT‑GTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGCGGGGAAAGGCTGCATTATGGGCAGTGCGCACCAGCGTCt   <  2:5263552/94‑1 (MQ=255)
                                                                                   ggATT‑GTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGCGGGGAAAGGCTGCATTATGGGCAGTGCGCACCAGCGTCTg  >  1:2418793/1‑94 (MQ=255)
                                                                                   ggATT‑GTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGCGGGGAAAGGCTGCATTATGGGCAGTGCGCACCAGCGTCTg  >  2:3299743/1‑94 (MQ=255)
                                                                                          |                                                                                       
GTCTGGAAGTGATGGCGGGCGGCGTTTC‑AAAGGCCATGCGCTGGAAG‑GCTGGCG‑‑‑‑AA‑‑‑‑CT‑GGCTACAG‑CTGAAGGATT‑G‑ATTGCGTT‑‑GTGACGGGATGAACGACGCCGAAATGCTGTCGATGGC‑GGGAAA‑GCTGCATTATGGGCAGTGGCCACCAGCGTCTG  >  NC_000913/4008345‑4008504

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 18 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output Only 200 of 231 total aligned reads displayed.
TTCAAAGGCCATGCGCTGGAAG‑GCTGGCG‑‑‑‑AACT‑G‑GCTACAG‑CTGAAGGATT‑G‑ATTGCGTT‑‑GTGACGGGATGAACGACGCCGAAATGCTGTCGATGGC‑‑GGG‑‑AAAG‑CTGCATTATGGGCAGTGGCCACCAGCGTCTGAAAGACCTTCATCCCG  >  NC_000913/4008370‑4008520
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tcaAAAGGCCATGCGCTGGAAGCGGTGGCGAAGAAACTGG‑GCTACAGCCTGAAGGATT‑GTATTGCGTTTGGTGACGGGATGAACGACGCCGAAA                                                                          >  SRR4302545.4767244/4‑94 (MQ=16)
 caAAAGGCCATGCGCTGGAAGCGGTGGCGAAGAAACTGG‑GCTACAGCCTGAAGGATT‑GTATTGCGTTTGGTGACGGGATGAACGACGCCGAAAT                                                                         <  SRR4302545.8387026/92‑1 (MQ=18)
 caAAAGGCCATGCGCTGGAAGCGGTGGCGAAGAAACTGG‑GCTACAGCCTGAAGGATT‑GTATTGCGTTTGGTGACGGGATGAACGACGCCGAAAT                                                                         <  SRR4302545.2575333/92‑1 (MQ=18)
 caAAAGGCCATGCGCTGGAAGCGGTGGCGAAGAAACTGG‑GCTACAGCCTGAAGGATT‑GTATTGCGTTTGGTGACGGGATGAACGACGCCGAAAT                                                                         >  SRR4302545.2354825/3‑94 (MQ=18)
 caAAAGGCCATGCGCTGGAAGCGGTGGCGAAGAAACTGG‑GCTACAGCCTGAAGGATT‑GTATTGCGTTTGGTGACGGGATGAACGACGCCGAAAT                                                                         <  SRR4302545.2348517/92‑1 (MQ=18)
 caAAAGGCCATGCGCTGGAAGCGGTGGCGAAGAAACTGG‑GCTACAGCCTGAAGGATT‑GTATTGCGTTTGGTGACGGGATGAACGACGCCGAAAT                                                                         <  SRR4302545.8579375/92‑1 (MQ=18)
 caAAAGGCCATGCGCTGGAAGCGGTGGCGAAGAAACTGG‑GCTACAGCCTGAAGGATT‑GTATTGCGTTTGGTGACGGGATGAACGACGCCGAAAT                                                                         <  SRR4302545.8480524/92‑1 (MQ=18)
 caAAAGGCCATGCGCTGGAAGCGGTGGCGAAGAAACTGG‑GCTACAGCCTGAAGGATT‑GTATTGCGTTTGGTGACGGGATGAACGACGCCGAAAT                                                                         <  SRR4302545.8435863/92‑1 (MQ=18)
  AAAAGGCCATGCGCTGGAAGCGGTGGCGAAGAAACTGG‑GCTACAGCCTGAAGGATT‑GTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATG                                                                        >  SRR4302545.3592584/1‑94 (MQ=19)
  AAAAGGCCATGCGCTGGAAGCGGTGGCGAAGAAACTGG‑GCTACAGCCTGAAGGATT‑GTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATG                                                                        >  SRR4302545.312860/1‑94 (MQ=19)
  AAAAGGCCATGCGCTGGAAGCGGTGGCGAAGAAACTGG‑GCTACAGCCTGAAGGATT‑GTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATG                                                                        >  SRR4302545.5616317/1‑94 (MQ=19)
  AAAAGGCCATGCGCTGGAAGCGGTGGCGAAGAAACTGG‑GCTACAGCCTGAAGGATT‑GTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATG                                                                        >  SRR4302545.874445/1‑94 (MQ=19)
   AAAGGCCATGCGCTGGAAGCGGTGGCGAAGAAACTGG‑GCTACAGCCTGAAGGATT‑GTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGC                                                                       <  SRR4302545.1035173/94‑1 (MQ=21)
     AGGCCATGCGCTGGAAGCGGTGGCGAAGAAACTGG‑GCTACAGCCTGAAGGATT‑GTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTG                                                                     >  SRR4302545.287453/1‑94 (MQ=23)
     AGGCCATGCGCTGGAAGCGGTGGCGAAGAAACTGG‑GCTACAGCCTGAAGGATT‑GTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTG                                                                     <  SRR4302545.5627053/94‑1 (MQ=23)
     AGGCCATGCGCTGGAAGCGGTGGCGAAGAAACTGG‑GCTACAGCCTGAAGGATT‑GTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTG                                                                     <  SRR4302545.10720851/94‑1 (MQ=23)
      tgccatgcgctggaagcggtggcgaagaaactgG‑GCTACAGCCTGAAGGATT‑GTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGT                                                                    >  SRR4302545.3143726/34‑94 (MQ=36)
      GGCCATGCGCTGGAAGCGGTGGCGAAGAAACTGG‑GCTACAGCCTGAAGGATT‑GTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGT                                                                    <  SRR4302545.6870088/94‑1 (MQ=25)
       GCCATGCGCTGGAAGCGGTGGCGAAGAAACTGG‑GCTACAGCCTGAAGGATT‑GTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTC                                                                   <  SRR4302545.5377252/94‑1 (MQ=26)
       GCCATGCGCTGGAAGCGGTGGCGAAGAAACTGG‑GCTACAGCCTGAAGGATT‑GTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTC                                                                   <  SRR4302545.7325608/94‑1 (MQ=26)
        CCATGCGCTGGAAGCGGTGGCGAAGAAACTGG‑GCTACAGCCTGAAGGATT‑GTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCG                                                                  <  SRR4302545.5176382/94‑1 (MQ=28)
        CCATGCGCTGGAAGCGGTGGCGAAGAAACTGG‑GCTACAGCCTGAAGGATT‑GTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCG                                                                  <  SRR4302545.10756070/94‑1 (MQ=28)
        CCATGCGCTGGAAGCGGTGGCGAAGAAACTGG‑GCTACAGCCTGAAGGATT‑GTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCG                                                                  <  SRR4302545.1999026/94‑1 (MQ=28)
         catgcgctggaagcggtggcgaagaaacggG‑GCTACAGCCTGAAGGATT‑GTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGA                                                                 <  SRR4302545.9545021/64‑1 (MQ=42)
          atgcgctggaagcggtggcgaagaaactgG‑GCTACAGCCTGAAGGATT‑GTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGAT                                                                >  SRR4302545.7973788/30‑94 (MQ=45)
          atgcgctggaagcggtggcgaagaaactgG‑GCTACAGCCTGAAGGATT‑GTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGAT                                                                <  SRR4302545.1925737/65‑1 (MQ=45)
          atgcgctggaagcggtggcgaagaaactgG‑GCTACAGCCTGAAGGATT‑GTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGAT                                                                <  SRR4302545.1609605/65‑1 (MQ=45)
          atgcgctggaagcggtggcgaagaaactgG‑GCTACAGCCTGAAGGATT‑GTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGAT                                                                <  SRR4302545.3770210/65‑1 (MQ=45)
          atgcgctggaagcggtggcgaagaaactgG‑GCTACAGCCTGAAGGATT‑GTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGAT                                                                <  SRR4302545.2072725/65‑1 (MQ=45)
          atgcgctggaagcggtggcgaagaaactgG‑GCTACAGCCTGAAGGATT‑GTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGAT                                                                >  SRR4302545.10257309/30‑94 (MQ=45)
          atgcgctggaagcggtggcgaagaaactgG‑GCTACAGCCTGAAGGATT‑GTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGAT                                                                >  SRR4302545.10257310/30‑94 (MQ=45)
          atgcgctggaagcggtggcgaagaaactgG‑GCTACAGCCTGAAGGATT‑GTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGAT                                                                <  SRR4302545.4225974/65‑1 (MQ=45)
          atgcgctggaagcggtggcgaagaaactgG‑GCTACAGCCTGAAGGATT‑GTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGAT                                                                >  SRR4302545.9191077/30‑94 (MQ=45)
           tgcgctggaagcggtggcgaagaaactgG‑GCTACAGCCTGAAGGATT‑GTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATG                                                               <  SRR4302545.8547751/66‑1 (MQ=47)
           tgcgctggaagcggtggcgaagaaactgG‑GCTACAGCCTGAAGGATT‑GTATTGCGTTTGGTGACAGGATGAACGACGCCGAAATGCTGTCGATG                                                               <  SRR4302545.6922890/66‑1 (MQ=33)
           tgcgctggaagcggtggcgaagaaacggG‑GCTACAGCCTGAAGGATT‑GTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATG                                                               <  SRR4302545.3225758/66‑1 (MQ=47)
            gcgctggaagcggtggcgaagaaactgG‑GCTACAGCCTGAAGGATT‑GTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGG                                                              >  SRR4302545.10067493/28‑94 (MQ=49)
            gcgctggaagcggtggcgaagaaactgG‑GCTACAGCCTGAAGGATT‑GTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGG                                                              <  SRR4302545.173343/67‑1 (MQ=49)
            gcgctggaagcggtggcgaagaaactgG‑GCTACAGCCTGAAGGATT‑GTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGG                                                              <  SRR4302545.8176748/67‑1 (MQ=49)
            gcgctggaagcggtggcgaagaaactgG‑GCTACAGCCTGAAGGATT‑GTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGG                                                              >  SRR4302545.250520/28‑94 (MQ=49)
             cgctggaagcggtggcgaagaaactgggctacagcctgaaggattgtcttgcggtTT‑‑GTGACGGGATGAACGACGCCGAAATGCTGTCGATGGG                                                             >  SRR4302545.9244871/56‑94 (MQ=60)
             cgctggaagcggtggcgaagaaactgG‑GCTACAGCCTGAAGGATT‑GTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGG                                                             >  SRR4302545.9244872/27‑94 (MQ=48)
             cgctggaagcggtggcgaagaaactgG‑GCTACAGCCTGAAGGATT‑GTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGC                                                             <  SRR4302545.1747799/68‑1 (MQ=51)
             cgctggaagcggtggcgaagaaactgG‑GCTACAGCCTGAAGGATT‑GTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGC                                                             <  SRR4302545.9103505/68‑1 (MQ=51)
              gctggaagcggtggcgaagaaactgG‑GCTACAGCCTGAAGGATT‑GTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGTTGTCGATGGC‑‑G                                                          >  SRR4302545.2127916/26‑94 (MQ=39)
              gctggaagcggtggcgaagaaactgG‑GCTACAGCCTGAAGGATT‑GTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGC‑‑G                                                          >  SRR4302545.8562300/26‑94 (MQ=53)
              gctggaagcggtggcgaagaaactgG‑GCTACAGCCTGAAGGATT‑GTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGC‑‑G                                                          <  SRR4302545.10832781/69‑1 (MQ=53)
              gctggaagcggtggcgaagaaactgG‑GCTACAGCCTGAAGGATT‑GTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGC‑‑G                                                          <  SRR4302545.6111778/69‑1 (MQ=53)
              gctggaagcgggggcgaagaaactgG‑GCTACAGCCTGAAGGATT‑GTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGGTGGC‑‑G                                                          >  SRR4302545.8562301/26‑94 (MQ=39)
               ctggaagcggtggcgaagaaactgG‑GCTACAGCCTGAAGGATT‑GTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGC‑‑GG                                                         >  SRR4302545.8077099/25‑94 (MQ=55)
                tggaagcggtggcgaagaaactgG‑GCTACAGCCTGAAGGATT‑GTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGC‑‑GGG                                                        >  SRR4302545.9437685/24‑94 (MQ=57)
                tggaagcggtggcgaagaaactgG‑GCTACAGCCTGAAGGATT‑GTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGC‑‑GGG                                                        <  SRR4302545.5270725/71‑1 (MQ=57)
                tggaagcggtggcgaagaaactgG‑GCTACAGCCTGAAGGATT‑GTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGC‑‑GGG                                                        >  SRR4302545.8654571/24‑94 (MQ=57)
                tggaagcggtggcgaagaaactgG‑GCTACAGCCTGAAGGATT‑GTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGC‑‑GGG                                                        <  SRR4302545.10079788/71‑1 (MQ=57)
                tggaagcggtggcgaagaaactgG‑GCTACAGCCTGAAGGATT‑GTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGC‑‑GGG                                                        <  SRR4302545.1372541/71‑1 (MQ=57)
                tggaagcggtggcgaagaaactgG‑GCTACAGCCTGAAGGATT‑GTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGC‑‑GGG                                                        >  SRR4302545.7317854/24‑94 (MQ=57)
                 ggaagcggtggcgaagaaactgG‑GCTACAGCCTGAAGGATT‑GTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGC‑‑GGG‑‑G                                                     <  SRR4302545.6428066/72‑1 (MQ=56)
                 ggaagcggtggcgaagaaactgG‑GCTACAGCCTGAAGGATT‑GTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGC‑‑GGG‑‑G                                                     <  SRR4302545.4147849/72‑1 (MQ=56)
                  tgaagcggtgtcgaagaatctggtctacagcCTGAAGGATTGG‑ATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGC‑‑GGG‑‑G                                                     <  SRR4302545.1588683/63‑1 (MQ=60)
                  gaagcggtggcgaagaaactgG‑GCTACAGCCTGAAGGATT‑GTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGC‑‑GGG‑‑GA                                                    >  SRR4302545.9454680/22‑94 (MQ=56)
                  gaagcggtggcgaagaaactgG‑GCTACAGCCTGAAGGATT‑GTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGC‑‑GGG‑‑GA                                                    >  SRR4302545.1487729/22‑94 (MQ=56)
                  gaagcggtggcgaagaaactgG‑GCTACAGCCTGAAGGATT‑GTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGC‑‑GGG‑‑GA                                                    <  SRR4302545.4514185/73‑1 (MQ=56)
                   aagcggtggcgaagaacctgG‑GCTACAGCCTGAAGGATT‑GTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGC‑‑GGG‑‑GAA                                                   <  SRR4302545.2267980/74‑1 (MQ=55)
                   aagcggtggcgaagaaactgG‑GCTACAGCCTGAAGGATT‑GTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGC‑‑GGG‑‑GAA                                                   <  SRR4302545.2524331/74‑1 (MQ=55)
                   aagcggtggcgaagaaactgG‑GCTACAGCCTGAAGGATT‑GTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGC‑‑GGG‑‑GAA                                                   <  SRR4302545.5455299/74‑1 (MQ=55)
                   aagcggtggcgaagaaactgG‑GCTACAGCCTGAAGGATT‑GTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGC‑‑GGG‑‑GAA                                                   <  SRR4302545.1008841/74‑1 (MQ=55)
                   aagcggtggcgaagaaactgG‑GCTACAGCCTGAAGGATT‑GTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGC‑‑GGG‑‑GAA                                                   >  SRR4302545.4116772/21‑94 (MQ=55)
                    agcggtggcgaagaaactgG‑GCTACAGCCTGAAGGATT‑GTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGCG‑GGG‑‑AAA                                                   <  SRR4302545.8738634/75‑1 (MQ=54)
                    agcggtggcgaagaaactgG‑GCTACAGCCTGAAGGATT‑GTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGCG‑GGG‑‑AAA                                                   >  SRR4302545.4913966/20‑94 (MQ=54)
                    agcggtggcgaagaaactgG‑GCTACAGCCTGAAGGATT‑GTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGCG‑GGG‑‑AAA                                                   <  SRR4302545.9144130/75‑1 (MQ=54)
                     gcggtggcgaagaaactgG‑GCTACAGCCTGAAGGATT‑GTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGCG‑GGG‑‑AAAG                                                  <  SRR4302545.4463206/76‑1 (MQ=53)
                     gcggtggcgaagaaactgG‑GCTACAGCCTGAAGGATT‑GTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGCG‑GGG‑‑AAAG                                                  <  SRR4302545.8626951/76‑1 (MQ=53)
                     gcggtggcgaagaaactgG‑GCTACAGCCTGAAGGATT‑GTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGCG‑GGG‑‑AAAG                                                  <  SRR4302545.7721502/76‑1 (MQ=53)
                     gcggtggcgaagaaactgG‑GCTACAGCCTGAAGGATT‑GTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGCG‑GGG‑‑AAAG                                                  <  SRR4302545.4463207/76‑1 (MQ=53)
                      cggtggcgaagaaactgG‑GCTACAGCCTGAAGGATT‑GTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGC‑‑GGGgaaagg                                                  >  SRR4302545.1879854/18‑88 (MQ=57)
                      cggtggcgaagaaactgG‑GCTACAGCCTGAAGGATT‑GTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGC‑‑GGGgaaagg                                                  <  SRR4302545.299968/77‑7 (MQ=57)
                      cggtggcgaagaaactgG‑GCTACAGCCTGAAGGATT‑GTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGC‑‑GGGgaaagg                                                  >  SRR4302545.7545326/18‑88 (MQ=57)
                       ggtggcgaagaaactgG‑GCTACAGCCTGAAGGATT‑GTATTGCGTTTGGTGACGGGATGAACGCCACCGAAATGCTGTCGATGGC‑‑GGagaaatgc                                                 >  SRR4302545.4473925/17‑86 (MQ=29)
                       ggtggcgaagaaactgG‑GCTACAGCCTGAAGGATT‑GTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGC‑‑GGGgaaaggc                                                 <  SRR4302545.5686161/78‑8 (MQ=57)
                       ggtggcgaagaaactgG‑GCTACAGCCTGAAGGATT‑GTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGC‑‑GGGgaaaggc                                                 <  SRR4302545.2116743/78‑8 (MQ=57)
                       ggtggcgaagaaactgG‑GCTACAGCCTGAAGGATT‑GTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGC‑‑GGGgaaaggc                                                 >  SRR4302545.3424649/17‑87 (MQ=57)
                       ggtggcgaagaaactgG‑GCTACAGCCTGAAGGATT‑GTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGC‑‑GGGgaaaggc                                                 >  SRR4302545.6965074/17‑87 (MQ=57)
                       ggtggcgaagaaactgG‑GCTACAGCCTGAAGGATT‑GTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGC‑‑GGGgaaaggc                                                 >  SRR4302545.2026337/17‑87 (MQ=57)
                        gtggcgaagaaactgG‑GCTACAGCCTGAAGGATT‑GTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGC‑‑GGtgaaaggct                                                <  SRR4302545.10696059/79‑10 (MQ=55)
                        gtggcgaagaaactgG‑GCTACAGCCTGAAGGATT‑GTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGC‑‑GGGgaaaggct                                                <  SRR4302545.189557/79‑9 (MQ=57)
                        gtggcgaagaaactgG‑GCTACAGCCTGAAGGATT‑GTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGC‑‑GGGgaaaggct                                                <  SRR4302545.2598604/79‑9 (MQ=57)
                         tggcgaagaaactgG‑GCTACAGCCTGAAGGATT‑GTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGC‑‑GGGgaaaggctg                                               <  SRR4302545.491815/80‑10 (MQ=57)
                         tggcgaagaaactgG‑GCTACAGCCTGAAGGATT‑GTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGC‑‑GGGgaaaggctg                                               <  SRR4302545.4970368/80‑10 (MQ=57)
                         tggcgaagaaactgG‑GCTACAGCCTGAAGGATT‑GTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGC‑‑GGGgaaaggctg                                               >  SRR4302545.5167863/15‑85 (MQ=57)
                         gggcgaagaaactgG‑GCTACAGCCTGAAGGATT‑GTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGC‑‑GGGgaaaggctg                                               <  SRR4302545.8742684/80‑10 (MQ=57)
                          ggcgaagaaactgG‑GCTACAGCCTGAAGGATT‑GTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGC‑‑GGGgaaaggctgc                                              >  SRR4302545.571596/14‑84 (MQ=57)
                          ggcgaagaaactgG‑GCTACAGCCTGAAGGATT‑GTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGC‑‑GGGgaaaggctgc                                              <  SRR4302545.329098/81‑11 (MQ=57)
                          ggcgaagaaactgG‑GCTACAGCCTGAAGGATT‑GTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGC‑‑GGGgaaaggctgc                                              <  SRR4302545.286979/81‑11 (MQ=57)
                          ggcgaagaaactgG‑GCTACAGCCTGAAGGATT‑GTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGC‑‑GGGgaaaggctgc                                              <  SRR4302545.4187247/81‑11 (MQ=57)
                          ggcgaagaaactgG‑GCTACAGCCTGAAGGATT‑GTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGC‑‑GGGgaaaggctgc                                              >  SRR4302545.3340190/14‑84 (MQ=57)
                           gcgaagaaactgG‑GCTACAGCCTGAAGGATT‑GTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGC‑‑GGGGAAAGG‑CTGCA                                            >  SRR4302545.5240300/13‑94 (MQ=50)
                           gcgaagaaactgG‑GCTACAGCCTGAAGGATT‑GTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGC‑‑GGGGAAAGG‑CTGCA                                            <  SRR4302545.2737884/82‑1 (MQ=50)
                            cgaagaaactgG‑GCTACAGCCTGAAGGATT‑GTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGT‑‑GGGGAAAGG‑CTGCAT                                           <  SRR4302545.5034143/83‑1 (MQ=40)
                            cgaagaaactgG‑GCTACAGCCTGAAGGATT‑GTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGC‑‑GGGGAAAGG‑CTGCAT                                           <  SRR4302545.5813065/83‑1 (MQ=49)
                            cgaagaaactgG‑GCTACAGCCTGAAGGATT‑GTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGC‑‑GGGGAAAGG‑CTGCAT                                           <  SRR4302545.778260/83‑1 (MQ=49)
                            cgaagaaactgG‑GCTACAGCCTGAAGGATT‑GTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGC‑‑GGGGAAAGG‑CTGCAT                                           >  SRR4302545.4203564/12‑94 (MQ=49)
                             gaagaaactgG‑GCTACAGCCTGAAGGATT‑GTATTGCGTTTGGTGACGGGATGAACGACGCTGAAATGCTGTCGATGGC‑‑GGGGAAAGG‑CTGCATT                                          <  SRR4302545.9350761/84‑1 (MQ=37)
                             gaagaaactgG‑GCTACAGCCTGAAGGATT‑GTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGC‑‑GGGGAAAGG‑CTGCATT                                          >  SRR4302545.4277279/11‑94 (MQ=49)
                             gaagaaactgG‑GCTACAGCCTGAAGGATT‑GTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGC‑‑GGGGAAAGG‑CTGCATT                                          >  SRR4302545.10371611/11‑94 (MQ=49)
                             gaagaaactgG‑GCTACAGCCTGAAGGATT‑GTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGC‑‑GGGGAAAGG‑CTGCATT                                          >  SRR4302545.10842559/11‑94 (MQ=49)
                             gaagaaactgG‑GCTACAGCCTGAAGGATT‑GTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGC‑‑GGGGAAAGG‑CTGCATT                                          >  SRR4302545.3702744/11‑94 (MQ=49)
                             gaagaaactgG‑GCTACAGCCTGAAGGATT‑GTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGC‑‑GGGGAAAGG‑CTGCATT                                          <  SRR4302545.10191557/84‑1 (MQ=49)
                             gaagaaactgG‑GCTACAGCCTGAAGGATT‑GTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGC‑‑GGGGAAAGG‑CTGCATT                                          >  SRR4302545.4757716/11‑94 (MQ=49)
                             gaagaaactgG‑GCTACAGCCTGAAGGATT‑GTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGC‑‑GGGGAAAGG‑CTGCATT                                          >  SRR4302545.1482517/11‑94 (MQ=49)
                              aagaaactgG‑GCTACAGCCTGAAGGATT‑GTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGC‑‑GGGgaaaggctggatta                                          >  SRR4302545.1375441/10‑80 (MQ=57)
                               agaaactgG‑GCTACAGCCTGAAGGATT‑GTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGC‑‑GGGGAAAGG‑CTGCATTAT                                        <  SRR4302545.2413800/86‑1 (MQ=48)
                               agaaactgG‑GCTACAGCCTGAAGGATT‑GTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGC‑‑GGGGAAAGG‑CTGCATTAT                                        >  SRR4302545.8437115/9‑94 (MQ=48)
                               agaaactgG‑GCTACAGCCTGAAGGATT‑GTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGC‑‑GGGGAAAGG‑CTGCATTAT                                        <  SRR4302545.4729605/86‑1 (MQ=48)
                               agaaactgG‑GCTACAGCCTGAAGGATT‑GTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGC‑‑GGGGAAAGG‑CTGCATTAT                                        <  SRR4302545.305200/86‑1 (MQ=48)
                                gaaactgG‑GCTACAGCCTGAAGGATT‑GTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGC‑‑GGGGAAAGG‑CTGCATTATG                                       >  SRR4302545.8468224/8‑94 (MQ=49)
                                gaaactgG‑GCTACAGCCTGAAGGATT‑GTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGC‑‑GGGGAAAGG‑CTGCATTATG                                       >  SRR4302545.1730896/8‑94 (MQ=49)
                                 aaactgG‑GCTACAGCCTGAAGGATT‑GTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGC‑‑GGGGAAAGG‑CTGCATTATGG                                      >  SRR4302545.7928784/7‑94 (MQ=51)
                                 aaactgG‑GCTACAGCCTGAAGGATT‑GTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGC‑‑GGGGAAAGG‑CTGCATTATGG                                      <  SRR4302545.10022409/88‑1 (MQ=51)
                                 aaactgG‑GCTACAGCCTGAAGGATT‑GTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGC‑‑GGGGAAAGG‑CTGCATTATGG                                      <  SRR4302545.7755049/88‑1 (MQ=51)
                                 aaactgG‑GCTACAGCCTGAAGGATT‑GTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGC‑‑GGGGAAAGG‑CTGCATTATGG                                      >  SRR4302545.8101187/7‑94 (MQ=51)
                                 aaactgG‑GCTACAGCCTGAAGGATT‑GTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGC‑‑GGGGAAAGG‑CTGCATTATGG                                      >  SRR4302545.3680290/7‑94 (MQ=51)
                                  AACTGG‑GCTACAGCCTGAAGGATT‑GTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGC‑‑GGGGAAAGG‑CTGCATTATGGG                                     <  SRR4302545.2835914/94‑1 (MQ=50)
                                  AACT‑GTGCTACAGCCTGAAGGATT‑GTAGTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGC‑‑GGGGAAAGG‑CTGCATTATGGG                                     <  SRR4302545.1171544/94‑1 (MQ=40)
                                    ctgG‑GCTACAGCCTGAAGGATT‑GTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGC‑‑GGGGAAAGG‑CTGCATTATGGGCA                                   >  SRR4302545.7987309/4‑94 (MQ=57)
                                    ctgG‑GCTACAGCCTGAAGGATT‑GTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGC‑‑GGGGAAAGG‑CTGCATTATGGGCA                                   <  SRR4302545.3599983/91‑1 (MQ=57)
                                    ctgG‑GCTACAGCCTGAAGGATT‑GTATTGCGTTTGGCGACGGGATGAACGAAGCCGAAATGCTGTCGATGGC‑‑GGGgaaaggctgcattattggcg                                    >  SRR4302545.4655664/4‑74 (MQ=31)
                                     tgG‑GCTACAGCCTGAAGGATT‑GTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGC‑‑GGGGAAAGG‑CTGCATTATGGGCAG                                  >  SRR4302545.1231582/3‑94 (MQ=58)
                                     G‑G‑GCTACAGCCTGAAGGATT‑GTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGC‑‑GGGGAAAGG‑CTGCATTATGGGCAGT                                 >  SRR4302545.105536/1‑94 (MQ=60)
                                       G‑GCTACAGCCTGAAGGATT‑GTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGC‑‑GGGGAAAGG‑CTGCCTTATGGGCAGTG                                >  SRR4302545.8497386/1‑94 (MQ=50)
                                       G‑GCTACAGCCTGAAGGATT‑GTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGC‑‑GGGGAAAGG‑CTGCATTATGGGCAGTG                                <  SRR4302545.1502884/94‑1 (MQ=60)
                                       G‑GCTACAGCCTGAAGGATT‑GTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGC‑‑GGGGAAAGG‑CTGCATTATGGGCAGTG                                >  SRR4302545.8497384/1‑94 (MQ=60)
                                         GCTACAGCCTGAAGGATT‑GTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGC‑‑GGGGAAAGG‑CTGCATTATGGGCAGTGC                               >  SRR4302545.1400189/1‑94 (MQ=60)
                                          CTACAGCCTGAAGGATT‑GTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGC‑‑GGGGAAAGG‑CTGCATTATGGGCAGTGcg                              <  SRR4302545.3351600/94‑3 (MQ=60)
                                          CTACAGCCTGAAGGATT‑GTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGC‑‑GGGGAAAGG‑CTGCATTATGGGCAGTGcg                              <  SRR4302545.6397730/94‑3 (MQ=60)
                                          CTACAGCCTGAAGGATT‑GTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGC‑‑GGGGAAAGG‑CTGCATTATGGGCAGTGcg                              <  SRR4302545.4869935/94‑3 (MQ=60)
                                          CTACAGCCTGAAGGATT‑GTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGC‑‑GGGGAAAGG‑CTGCATTATGGGCAGTGcg                              >  SRR4302545.10718452/1‑92 (MQ=60)
                                          CTACAGCCTGAAGGATT‑GTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGC‑‑GGGGAAAGG‑CTGCATTATGGGCAGTGcg                              >  SRR4302545.5711976/1‑92 (MQ=60)
                                          CTACAGCCTGAAGGATT‑GTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGC‑‑GGGGAAAGG‑CTGCATTATGGGCAGTGcg                              >  SRR4302545.2681045/1‑92 (MQ=60)
                                           TACAGCCTGAAGGATT‑GTATTGCGTTTGGTGACGGGCTGAACGACGCCGAAATGCTGTCGATGGC‑‑GGGGAGAGG‑CTGCATTATGGGCAGTGGGC                             >  SRR4302545.7445607/1‑94 (MQ=40)
                                            ACAGCCTGAAGGATT‑GTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGC‑‑GGGGAAAGG‑CTGCATTATGGGCAGTGcgca                            >  SRR4302545.9250123/1‑90 (MQ=60)
                                            ACAGCCTGAAGGATT‑GTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGC‑‑GGGGAAAGG‑CTGCATTATGGGCAGTGcgca                            >  SRR4302545.10093443/1‑90 (MQ=60)
                                            ACAGCCTGAAGGATT‑GTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGC‑‑GGGGAAAGG‑CTGCATTATGGGCAGTGcgca                            <  SRR4302545.1585454/94‑5 (MQ=60)
                                             CAGCCTGAAGGATT‑GTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGCGTGGC‑‑AAGG‑CTGCATTATGGGCAGTGcgtac                           >  SRR4302545.9405280/1‑89 (MQ=51)
                                             CAGCCTGAAGGATT‑GTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGC‑‑GGGGAAAGG‑CTGCATTATGGGCAGTGcgcac                           >  SRR4302545.1896861/1‑89 (MQ=60)
                                             CAGCCTGAAGGATT‑GTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGC‑‑GGGGAAAGG‑CTGCATTATGGGCAGTGcgcac                           >  SRR4302545.3956803/1‑89 (MQ=60)
                                             CAGCCTGAAGGATT‑GTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGC‑‑GGGGAAAGG‑CTGCATTATGGGCAGTGcgcac                           >  SRR4302545.3255126/1‑89 (MQ=60)
                                             CAGCCTGAAGGATT‑GTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGC‑‑GGGGAAAGG‑CTGCATTATGGGCAGTGcgcac                           >  SRR4302545.6163185/1‑89 (MQ=60)
                                             CAGCCTGAAGGATT‑GTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGC‑‑GGGGAAAGG‑CTGCATTATGGGCAGTGcgcac                           >  SRR4302545.8713037/1‑89 (MQ=60)
                                              agcCTGAAGGATT‑GTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGC‑‑GGGGAAAGG‑CTGCATTATGGGCAGTGCGCACC                          >  SRR4302545.4746905/4‑94 (MQ=60)
                                              agcCTGAAGGATT‑GTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGC‑‑GGGGAAAGG‑CTGCATTATGGGCAGTGCGCACC                          >  SRR4302545.4171883/4‑94 (MQ=60)
                                              agcCTGAAGGATT‑GTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGC‑‑GGGGAAAGG‑CTGCATTATGGGCAGTGCGCACC                          <  SRR4302545.2715239/91‑1 (MQ=60)
                                              AC‑CTGAAGGATT‑GTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGC‑‑GGGGAAAGG‑CTGCATTATGGGCAGTGCGCACCA                         <  SRR4302545.2610471/94‑1 (MQ=60)
                                               gcCTGAAGGATT‑GTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGC‑‑GGGGAAAGG‑CTGCATTATGGGCAGTGCGCACCA                         >  SRR4302545.768570/3‑94 (MQ=60)
                                               C‑CTGAAGGATT‑GTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGC‑‑GGGGAAAGG‑CTGCATTATGGGCAGTGCGCACCAG                        >  SRR4302545.4917408/1‑94 (MQ=60)
                                               C‑CTGAAGGATT‑GTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGC‑‑GGGGAAAGG‑CTGCATTATGGGCAGTGCGCACCAG                        <  SRR4302545.4977374/94‑1 (MQ=60)
                                                 CTGAAGGATT‑GTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGC‑‑GGGGAAAGG‑CTGCATTATGGGCAGTGcgcaccagg                       >  SRR4302545.8960570/1‑85 (MQ=60)
                                                 CTGAAGGATT‑GTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGC‑‑GGGGAAAGG‑CTGCATTATGGGCAGTGCGCACCAGC                       >  SRR4302545.3289969/1‑94 (MQ=60)
                                                 CTGAAGGATT‑GTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGC‑‑GGGGAAAGG‑CTGCATTATGGGCAGTGCGCACCAGC                       <  SRR4302545.553713/94‑1 (MQ=60)
                                                  TGAAGGATT‑GTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGC‑‑GGGGAAAGG‑CTGCATTATGGGCAGTGCGCACCAGCG                      <  SRR4302545.6617634/94‑1 (MQ=57)
                                                  TGAAGGATT‑GTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGC‑‑GGGGAAAGG‑CTGCATTATGGGCAGTGCGCACCAGCG                      >  SRR4302545.6798561/1‑94 (MQ=57)
                                                  TGAAGGATT‑GTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGC‑‑GGGGAAAGG‑CTGCATTATGGGCAGTGCGCACCAGCG                      >  SRR4302545.2397041/1‑94 (MQ=57)
                                                   GAAGGATT‑GTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGC‑‑GGGGAAAGG‑CTGCATTATGGGCAGTGCGCACCAGCGT                     >  SRR4302545.6087898/1‑94 (MQ=57)
                                                    AAGGATT‑GTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGC‑‑GGGGAAAGG‑CTGCATTATGGGCAGTGCGCACCAGCGTC                    >  SRR4302545.10443577/1‑94 (MQ=57)
                                                     AGGATT‑GTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGC‑‑GGGGAAAGG‑CTGCTTTATGGGCAGTGCGCACCAGCGTCT                   <  SRR4302545.10450907/94‑1 (MQ=46)
                                                     AGGATT‑GTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGC‑‑GGGGAAAGG‑CTGCATTATGGGCAGTGCGCACCAGCGTCT                   >  SRR4302545.6879448/1‑94 (MQ=57)
                                                      GGATT‑GTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGC‑‑GGGGAAAGG‑CTGCATTATGGGCAGTGCGCACCAGCGTCTG                  >  SRR4302545.2704431/1‑94 (MQ=60)
                                                      GGATT‑GTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGC‑‑GGGGAAAGG‑CTGCATTATGGGCAGTGCGCACCAGCGTCTG                  >  SRR4302545.1541333/1‑94 (MQ=60)
                                                      GGATT‑GTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGC‑‑GGGGAAAGG‑CTGCATTATGGGCAGTGCGCACCAGCGTCTG                  >  SRR4302545.2477397/1‑94 (MQ=60)
                                                      GGATT‑GTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGC‑‑GGGGAAAGG‑CTGCATTATGGGCAGTGCGCACCAGCGTCTG                  <  SRR4302545.5730486/94‑1 (MQ=60)
                                                      GGATT‑GTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGC‑‑GGGGAAAGG‑CTGCATTATGGGCAGTGCGCACCAGCGTCTG                  >  SRR4302545.3464165/1‑94 (MQ=60)
                                                       GATT‑GTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGC‑‑GGGGAAAGG‑CTGCATTATGGGCAGTGCGCACCAGCGTCTGA                 >  SRR4302545.2626314/1‑94 (MQ=60)
                                                       GATT‑GTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGC‑‑GGGGAAAGG‑CTGCATTATGGGCAGTGCGCACCAGCGTCTGA                 <  SRR4302545.3728847/94‑1 (MQ=60)
                                                        ATT‑GTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGC‑‑GGGGAAAGG‑CTGCATTATGGGCAGTGCGCACCCGCGTCTGAA                >  SRR4302545.5170333/1‑94 (MQ=53)
                                                        ATT‑GTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGC‑‑GGGGAAAGG‑CTGCATTATGGGCAGTGCGCACCAGCGTCTGAA                <  SRR4302545.403852/94‑1 (MQ=60)
                                                         TT‑GTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGC‑‑GGGGAAAGG‑CTGCATTATGGGCAGTGCGCACCAGCGTCTGAAA               >  SRR4302545.6020995/1‑94 (MQ=60)
                                                         TT‑GTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGC‑‑GGGGAAAGG‑CTGCATTATGGGCAGTGCGCACCAGCGTCTGAAA               <  SRR4302545.2336932/94‑1 (MQ=60)
                                                           tattgcgttTT‑‑GTGACGGGATGAACGACGCCGAAATGCTGTCGATGGC‑‑GGGGAAAGG‑CTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGAC            <  SRR4302545.3214656/85‑1 (MQ=60)
                                                            T‑ATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGC‑‑GGGGAAAGG‑CTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGAC            >  SRR4302545.10084193/1‑94 (MQ=60)
                                                            T‑ATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGC‑‑GGGGAAAGG‑CTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGAC            >  SRR4302545.5551456/1‑94 (MQ=60)
                                                            T‑ATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGC‑‑GGGGAAAGG‑CTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGAC            >  SRR4302545.2864931/1‑94 (MQ=60)
                                                            T‑ATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGC‑‑GGGGAAAGG‑CTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGAC            <  SRR4302545.4523898/94‑1 (MQ=60)
                                                              ATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGC‑‑GGGGAAAGG‑CTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACC           <  SRR4302545.9567710/94‑1 (MQ=60)
                                                              ATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGC‑‑GGGGAAAGG‑CTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACC           <  SRR4302545.2717689/94‑1 (MQ=60)
                                                              ATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGC‑‑GGGGAAAGG‑CTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACC           <  SRR4302545.4377710/94‑1 (MQ=60)
                                                               TTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGC‑‑GGGGAAAGG‑CTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCT          <  SRR4302545.811714/94‑1 (MQ=60)
                                                               TTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGC‑‑GGGGAAAGG‑CTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCT          >  SRR4302545.4960882/1‑94 (MQ=60)
                                                                TGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGC‑‑GGGGAAAGG‑CTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTT         <  SRR4302545.7532902/94‑1 (MQ=60)
                                                                 GCGTTTGGTGAGGGGATGAACGACGCCTAAATGCTGTCGATGGC‑‑GGGGAAAGG‑CTGCAATATGGGCAGTGCGGACCAGCGTGTGAAAGACCTTC        <  SRR4302545.9817450/94‑1 (MQ=28)
                                                                  CGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGC‑‑GGGGAAAGG‑CTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCA       <  SRR4302545.9970350/94‑1 (MQ=60)
                                                                  CGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGC‑‑GGGGAAAGG‑CTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCA       >  SRR4302545.2901743/1‑94 (MQ=60)
                                                                  CGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGC‑‑GGGGAAAGG‑CTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCA       <  SRR4302545.5922760/94‑1 (MQ=60)
                                                                  CGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGC‑‑GGGGAAAGG‑CTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCA       >  SRR4302545.6614716/1‑94 (MQ=60)
                                                                   GTTG‑GTGACGGGATGAACGACGCCGAAATGCTGTCGATGGC‑‑GGGGAAAGG‑CTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATC     <  SRR4302545.8057022/94‑1 (MQ=60)
                                                                    TG‑‑GTGACGGGATGAACGACGCCGAAATGCTGTCGATGGC‑‑GGGGAAAGG‑CTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCC   <  SRR4302545.1392340/94‑1 (MQ=60)
                                                                    TG‑‑GTGACGGGATGAACGACGCCGAAATGCTGTCGATGGC‑‑GGGGAAAGG‑CTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCC   <  SRR4302545.3685134/94‑1 (MQ=60)
                                                                    TG‑‑GTGACGGGATGAACGACGCCGAAATGCTGTCGATGGC‑‑GGGGAAAGG‑CTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCC   <  SRR4302545.2105337/94‑1 (MQ=60)
                                                                    TG‑‑GTGACGGGATGAACGACGCCGAAATGCTGTCGATGGC‑‑GGGGAAAGG‑CTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCC   <  SRR4302545.7591135/94‑1 (MQ=60)
                                                                     T‑‑TTGACGAGATGAGCGACGCCGAAATGCTGTCGATGGCG‑GGG‑‑AAAGCCTGCATTATGGGCAGTTCGCACCAGCGTCTGAAAGACCTTCATCCCG  <  SRR4302545.5616814/94‑1 (MQ=57)
                                                                     G‑‑GTGACGGGATGAACGACGCCGAAATGCTGTCGATGGC‑‑GGGGAAAGG‑CTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCG  >  SRR4302545.8902310/1‑94 (MQ=60)
                                                                     G‑‑GTGACGGGATGAACGACGCCGAAATGCTGTCGATGGC‑‑GGGGAAAGG‑CTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCG  >  SRR4302545.5632409/1‑94 (MQ=60)
                                                                     |                                                                                                  
TTCAAAGGCCATGCGCTGGAAG‑GCTGGCG‑‑‑‑AACT‑G‑GCTACAG‑CTGAAGGATT‑G‑ATTGCGTT‑‑GTGACGGGATGAACGACGCCGAAATGCTGTCGATGGC‑‑GGG‑‑AAAG‑CTGCATTATGGGCAGTGGCCACCAGCGTCTGAAAGACCTTCATCCCG  >  NC_000913/4008370‑4008520

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 7 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑