Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I1 R1 647 270.1 21258240 98.4% 20918108 94.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 4,008,491 2 bp→CG intergenic (+58/‑174) b4555 → / → yigM hypothetical protein/hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,008,4910GC99.8% 1687.6 / NA 490intergenic (+58/‑175)b4555/yigMhypothetical protein/hypothetical protein
Reads supporting (aligned to +/- strand):  ref base G (0/0);  major base C (257/232);  minor base A (1/0);  total (258/232)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
*NC_0009134,008,4920CG100.0% 1657.3 / NA 479intergenic (+59/‑174)b4555/yigMhypothetical protein/hypothetical protein
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base G (251/228);  total (251/228)

Only 100 of 496 total aligned reads displayed.

CAGCTGAAGGATTG‑ATTGCGTT‑‑GTGACGGGATGAACGACGCCGAAATGCTGTCGATGGC‑GGGAAA‑G‑CTGCATTATGGGCAGTGGCCACCAGCGTCTGAAAGACCTTCATCCCGAGCT‑GAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTAAACTC  >  NC_000913/4008408‑4008578
                                                                                         ||                                                                                       
agccTGAAGGATTGTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGCGGGGAAAGG‑CTGCATTATGGGCAGTGCgcacc                                                                                     >  2:8473482/4‑94 (MQ=255)
agccTGAAGGATTGTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGCGGGGAAAGG‑CTGCATTATGGGCAGTGCgcacc                                                                                     >  1:279937/4‑94 (MQ=255)
agccTGAAGGATTGTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGCGGGGAAAGG‑CTGCATTATGGGCAGTGCgcacc                                                                                     >  1:4628565/4‑94 (MQ=255)
  ccTGAAGGATTGTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGCGGGGAAAGG‑CTGCATTATGGGCAGTGCGCACCAg                                                                                   >  2:1230917/2‑94 (MQ=255)
   cTGAAGGATTGTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGCGGGGAAAGG‑CTGCATTATGGGCAGTGCGCACCAgg                                                                                  >  1:8707946/1‑93 (MQ=255)
   cTGAAGGATTGTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGCGGGGAAAGG‑CTGCATTATGGGCAGTGCGCACCAGc                                                                                  >  2:8592412/1‑94 (MQ=255)
    tGAAGGATTGTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGCGGGGAAAGG‑CTGCATTATGGGCAGTGCGCACCAGCg                                                                                 >  1:6617264/1‑94 (MQ=255)
     gAAGGATTGTATTGCGTTTGGTGACGGGATGAACGTCGCCGAAATGCTGTCGATGGCGGGGAAAGG‑CTGCATTATGGGCAGTGCGCACCAGCGt                                                                                <  1:2599168/94‑1 (MQ=255)
       aGGATTGTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGCGGGGAAAGG‑CTGCATTATGGGCAGTGCGCACCAGCGTCt                                                                              >  2:6968644/1‑94 (MQ=255)
       aGGATTGTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGCGGGGAAAGG‑CTGCATTATGGGCAGTGCGCACCAGCGTCt                                                                              >  2:2371359/1‑94 (MQ=255)
       aGGATTGTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGCGGGGAAAGG‑CTGCATTATGGGCAGTGCGCACCAGCGTCt                                                                              <  2:5263552/94‑1 (MQ=255)
                       tggtgACGGGATGAACGACGCCGAAATGCTGTCGATGGCGGGGAAAGG‑CTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATccc                                                              <  1:1361868/92‑1 (MQ=255)
                       tggtgACGGGATGAACGACGCCGAAATGCTGTCGATGGCGGGGAAAGG‑CTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATccc                                                              <  2:3120830/92‑1 (MQ=255)
                        ggtgACGGGATGAACGACGCCGAAATGCTGTCGATGGCGGGGAAAGG‑CTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCg                                                             <  2:9879094/93‑1 (MQ=255)
                        ggtgACGGGATGAACGACGCCGAAATGCTGTCGATGGCGGGGAAAGG‑CTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCg                                                             >  1:5488157/2‑94 (MQ=255)
                        ggtgACGGGATGAACGACGCCGAAATGCTGTCGATGGCGGGGAAAGG‑CTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCg                                                             <  2:6320590/93‑1 (MQ=255)
                          tgACGGGATGAACGACGCCGAAATGCTGTCGATGGCGGGGAAAGG‑CTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAg                                                           >  2:395505/1‑94 (MQ=255)
                          tgACGGGATGAACGACGCCGAAATGCTGTCGATGGCGGGGAAAGG‑CTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAg                                                           >  2:579446/1‑94 (MQ=255)
                            aCGGGATGAACGACGCCGAAATGCTGTCGATGGCGGGGAAAGG‑CTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGAACTTCATCCCGAGCt                                                         >  1:350022/1‑94 (MQ=255)
                              gggATGAACGACGCCGAAATGCTGTCGATGGCGGGGAAAGG‑CTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCT‑gg                                                      >  1:3290467/1‑93 (MQ=255)
                              gggATGAACGACGCCGAAATGCTGTCGATGGCGGGGAAAGG‑CTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCT‑gg                                                      >  1:2128916/1‑93 (MQ=255)
                                 aTGAACGACGCCGAAATGCTGTCGATGGCGGGGAAAGG‑CTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCT‑ggaag                                                   <  2:8914821/94‑5 (MQ=255)
                                   gAACGACGCCGAAATGCTGTCGATGGCGGGGAAAGG‑CTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTg                                                  >  1:2990349/1‑94 (MQ=255)
                                    aaCGACGCCGAAATGCTGTCGATGGCGGGGAAAGG‑CTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTgt                                                 >  2:8836198/1‑93 (MQ=255)
                                     acgacgCCGAAATGCTGTCGATGGCGGGGAAAGG‑CTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGAt                                                <  1:3114285/94‑1 (MQ=255)
                                      cgacgCCGAAATGCTGTCGATGGCGGGGAAAGG‑CTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGAtt                                               >  2:120205/1‑94 (MQ=255)
                                       gacgCCGAAATGCTGTCGATGGCGGGGAAAGG‑CTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTg                                              <  1:6213636/94‑1 (MQ=255)
                                        acgCCGAAATGCTGTCGATGGCGGGGAAATG‑CTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTgg                                             >  1:8971720/1‑94 (MQ=255)
                                        acgCCGAAATGCTGTCGATGGCGGGGAAAGG‑CTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTgg                                             >  1:438692/1‑94 (MQ=255)
                                        acgCCGAAATGCTGTCGATGGCGGGGAAAGG‑CTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTgg                                             >  1:4076240/1‑94 (MQ=255)
                                          gCCGAAATGCTGTCGATGGCGGGGAAAGG‑CTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTa                                           >  1:1002325/1‑94 (MQ=255)
                                           ccGAAATGCTGTCGATGGCGGGGAAAGG‑CTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTAc                                          <  1:2499528/94‑1 (MQ=255)
                                            cGAAATGCTGTCGATGGCGGGGAAAGG‑CTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACt                                         <  1:5866674/94‑1 (MQ=255)
                                            cGAAATGCTGTCGATGGCGGGGAAAGG‑CTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACt                                         <  2:6011273/94‑1 (MQ=255)
                                            cGAAATGCTGTCGATGGCGGGGAAAGG‑CTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACt                                         >  1:9038677/1‑94 (MQ=255)
                                             gAAATGCTGTCGATGGCGGGGAAAGG‑CTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTa                                        >  2:5040869/1‑94 (MQ=255)
                                             gAAATGCTGTCGATGGCGGGGAAAGG‑CTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTa                                        >  2:5591064/1‑94 (MQ=255)
                                             caaaTGCTGTCGATGGCGGGGAAAGG‑CTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTa                                        <  2:1744692/93‑1 (MQ=255)
                                              aaaTGCTGTCGATGGCGGGGAAAGG‑CTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTaa                                       <  2:1049617/94‑1 (MQ=255)
                                               aaTGCTGTCGATGGCGGGGAAAGG‑CTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAAt                                      >  2:1402694/1‑94 (MQ=255)
                                                aTGCTGTCGATGGCGGGGAAAGG‑CTGCATTATGGGCAGTTCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGGTTGAGACTAATg                                     >  2:3547064/1‑94 (MQ=255)
                                                aTGCTGTCGATGGCGGGGAAAGG‑CTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATg                                     <  1:2043956/94‑1 (MQ=255)
                                                aTGCTGTCGATGGCGGGGAAAGG‑CTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATg                                     <  1:7484825/94‑1 (MQ=255)
                                                aTGCTGTCGATGGCGGGGAAAGG‑CTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATg                                     >  1:117984/1‑94 (MQ=255)
                                                 tGCTGTCGATGGCGGGGAAAGG‑CTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGc                                    >  2:2479878/1‑94 (MQ=255)
                                                   cTGTCGATGGCGGGGAAAGG‑CTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCcg                                  >  2:9519735/1‑94 (MQ=255)
                                                   cTGTCGATGGCGGGGAAAGG‑CTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCcg                                  <  2:6543877/94‑1 (MQ=255)
                                                   cTGTCGATGGCGGGGAAAGG‑CTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCcg                                  <  1:5038496/94‑1 (MQ=255)
                                                   cTGTCGATGGCGGGGAAAGG‑CTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCcg                                  <  2:4362211/94‑1 (MQ=255)
                                                   cTGTCGATGGCGGGGAAAGG‑CTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCcg                                  <  2:8723267/94‑1 (MQ=255)
                                                   cTGTCGATGGCGGGGAAA‑GTCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCcg                                  <  1:773177/94‑1 (MQ=255)
                                                    tGTCGATGGCGGGGAAAGG‑CTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCcga                                 >  2:1036164/1‑94 (MQ=255)
                                                     gTCGATGGCGGGGAAAGG‑CTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCcgac                                <  1:6993022/94‑1 (MQ=255)
                                                     gTCGATGGCGGGGAAAGG‑CTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCcgac                                <  2:4735414/94‑1 (MQ=255)
                                                     gTCGATGGCGGGGAAAGG‑CTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCcgac                                <  2:2784094/94‑1 (MQ=255)
                                                     gTCGATGGCGGGGAAAGG‑CTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCcgac                                >  2:7709297/1‑94 (MQ=255)
                                                      tCGATGGCGGGGAAAGG‑CTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCcgacg                               >  2:6744316/1‑94 (MQ=255)
                                                      tCGATGGCGGGGAAAGG‑CTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCcgacg                               <  2:9180142/94‑1 (MQ=255)
                                                       cGATGGCGGGGAAATG‑CTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCcgacga                              <  2:2539768/94‑1 (MQ=255)
                                                       cGATGGCGGGGAAAGG‑CTGCATTCTGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCcgacga                              >  1:6720739/1‑94 (MQ=255)
                                                       cGATGGCGGGGAAAGG‑CTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCcgacga                              >  2:1668845/1‑94 (MQ=255)
                                                       cGATGGCGGGGAAAGG‑CTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCcgacga                              >  1:2391484/1‑94 (MQ=255)
                                                        gATGGCGGGGAAAGG‑CTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCgacgac                             >  1:7342128/1‑94 (MQ=255)
                                                        gATGGCGGGGAAAGG‑CTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCgacgac                             <  2:4606483/94‑1 (MQ=255)
                                                        gATGGCGGGGAAAGG‑CTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCgacgac                             <  1:5563810/94‑1 (MQ=255)
                                                        gATGGCGGGGAAAGG‑CTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCgacgac                             <  1:6335199/94‑1 (MQ=255)
                                                            gcggggAAAGG‑CTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCgg                         <  2:2928309/91‑1 (MQ=255)
                                                            gcggggAAAGG‑CTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCgg                         <  2:8242605/91‑1 (MQ=255)
                                                            gcggggAAAGG‑CTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCgg                         <  2:802013/91‑1 (MQ=255)
                                                             gggggAAAGG‑CTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGt                        <  2:7210574/92‑1 (MQ=255)
                                                              ggggAAAGG‑CTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTg                       >  2:497163/2‑94 (MQ=255)
                                                              ggggAAAGG‑CTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTg                       <  2:7465639/93‑1 (MQ=255)
                                                              ggggAAAGG‑CTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTg                       >  2:3310511/2‑94 (MQ=255)
                                                              ggggAAAGG‑CTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTACATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTg                       >  1:1710749/2‑94 (MQ=255)
                                                               gggAAAGG‑CTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGc                      >  1:10552508/1‑94 (MQ=255)
                                                                ggAAAGG‑CTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGcc                     <  2:7873783/94‑1 (MQ=255)
                                                                ggAAAGG‑CTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGcc                     >  2:6142097/1‑94 (MQ=255)
                                                                ggAAAGG‑CTGCATTATGGCCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGcc                     >  1:8544480/1‑94 (MQ=255)
                                                                  aaagg‑CTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGc                   >  2:2282763/5‑94 (MQ=255)
                                                                  aaagg‑CTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGc                   >  2:3690696/5‑94 (MQ=255)
                                                                   aagg‑CTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCa                  <  1:9733310/91‑1 (MQ=255)
                                                                    agg‑CTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCAt                 >  1:8382643/3‑94 (MQ=255)
                                                                    agg‑CTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCAt                 >  2:4159647/3‑94 (MQ=255)
                                                                     gg‑CTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCAtt                >  2:2091376/2‑94 (MQ=255)
                                                                      g‑CTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTa               >  1:7956812/1‑94 (MQ=255)
                                                                        cTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTAt              <  2:10085498/94‑1 (MQ=255)
                                                                        cTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTAt              >  1:9119220/1‑94 (MQ=255)
                                                                         tGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATc             >  2:980116/1‑94 (MQ=255)
                                                                         tGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATc             >  1:3748613/1‑94 (MQ=255)
                                                                         tGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATc             >  2:10041028/1‑94 (MQ=255)
                                                                           cATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTg           >  2:5366808/1‑94 (MQ=255)
                                                                           cATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTg           <  1:10210637/94‑1 (MQ=255)
                                                                            aTTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGc          <  1:10087239/94‑1 (MQ=255)
                                                                            aTTATGGGCAGGGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGc          <  1:8247893/94‑1 (MQ=255)
                                                                             ttATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCg         >  1:4921489/1‑94 (MQ=255)
                                                                              tATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGt        <  1:6010805/94‑1 (MQ=255)
                                                                               aTGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTa       >  2:1802914/1‑94 (MQ=255)
                                                                                tGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTaa      >  1:4735723/1‑94 (MQ=255)
                                                                                  ggCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTAAAc    <  1:5599029/94‑1 (MQ=255)
                                                                                    cAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGGGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTAAActc  >  1:4006741/1‑94 (MQ=255)
                                                                                         ||                                                                                       
CAGCTGAAGGATTG‑ATTGCGTT‑‑GTGACGGGATGAACGACGCCGAAATGCTGTCGATGGC‑GGGAAA‑G‑CTGCATTATGGGCAGTGGCCACCAGCGTCTGAAAGACCTTCATCCCGAGCT‑GAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTAAACTC  >  NC_000913/4008408‑4008578

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 18 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output Only 200 of 354 total aligned reads displayed.
CGGGATGAACGACGCCGAAATGCTGTCGATGG‑C‑G‑G‑G‑‑AAA‑G‑CTGCATTATGGGCAGTGGCCACCAGCGTCTGAAAGACCTTCATCCCGAGCT‑GAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTAAACTCTATTTATCGTAATCGTTCTTTATTTGGTCAGTTGTCAACC  >  NC_000913/4008434‑4008618
                                                                                                    |                                                                                             
TGGGATGAACGACGCCGAAATGCTGTCGATGG‑CGG‑G‑G‑‑AAA‑GCCTGCATTACGGGCAGTGCGCACCAGGGTCTGAAAGACCTTCACCCCGAGCT‑G                                                                                               >  SRR4302545.8111884/1‑94 (MQ=60)
CGGGATGAACGACGCCGAAATGCTGTCGATGG‑C‑G‑G‑GGAAAG‑G‑CTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGGGCT‑G                                                                                               >  SRR4302545.6892215/1‑94 (MQ=60)
CGGGATGAACGACGCCGAAATGCTGTCGATGG‑C‑G‑G‑GGAAAG‑G‑CTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCT‑G                                                                                               <  SRR4302545.6440780/94‑1 (MQ=60)
CGGGATGAACGACGCCGAAATGCTGTCGATGG‑C‑G‑G‑GGAAAG‑G‑CTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCT‑G                                                                                               >  SRR4302545.174145/1‑94 (MQ=60)
 GGGATGAACGACGCCGAAATGCTGTCGATGG‑C‑G‑G‑GGAAAG‑G‑CTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCT‑GG                                                                                              >  SRR4302545.3371016/1‑94 (MQ=60)
 GGGATGAACGACGCCGAAATGCTGTCGATGG‑C‑G‑G‑GGAAAG‑G‑CTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCT‑GG                                                                                              >  SRR4302545.5073063/1‑94 (MQ=60)
 GGGATGAACGACGCCGAAATGCTGTCGATGG‑C‑G‑G‑GGAAAG‑G‑CTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCT‑GG                                                                                              >  SRR4302545.8352247/1‑94 (MQ=60)
 GGGATGAACGACGCCGAAATGCTGTCGATGG‑C‑G‑G‑GGAAAG‑G‑CTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCT‑GG                                                                                              >  SRR4302545.2181143/1‑94 (MQ=60)
  GGATGAACGACGCCGAAATGCTGTCGATGG‑C‑G‑G‑GGAAAG‑G‑CTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCT‑GGA                                                                                             >  SRR4302545.6493858/1‑94 (MQ=60)
   GATGAACGACGCCGAAATGCTGTCGATGG‑C‑G‑G‑GGAAAG‑G‑CTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCT‑Ggaa                                                                                            <  SRR4302545.2172976/94‑4 (MQ=60)
   GATGAACGACGCCGAAATGCTGTCGATGG‑C‑G‑G‑GGAAAG‑G‑CTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCT‑Ggaa                                                                                            <  SRR4302545.4207168/94‑4 (MQ=60)
    ATGAACGACGCCGAAATGCTGTCGATGG‑C‑G‑G‑GGAAAG‑G‑CTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAG                                                                                            >  SRR4302545.4766774/1‑94 (MQ=60)
    ATGAACGACGCCGAAATGCTGTCGATGG‑C‑G‑G‑GGAAAG‑G‑CTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAG                                                                                            >  SRR4302545.2531533/1‑94 (MQ=60)
    ATGAACGACGCCGAAATGCTGTCGATGG‑C‑G‑G‑GGAAAG‑G‑CTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAG                                                                                            >  SRR4302545.172689/1‑94 (MQ=60)
    ATGAACGACGCCGAAATGCTGTCGATGG‑C‑G‑G‑GGAAAG‑G‑CTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAG                                                                                            <  SRR4302545.5735191/94‑1 (MQ=60)
     TGAACGACGCCGAAATGCTGTCGATGG‑C‑G‑G‑GGAAAG‑G‑CTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGT                                                                                           <  SRR4302545.771198/94‑1 (MQ=60)
     TGAACGACGCCGAAATGCTGTCGATGG‑C‑G‑G‑GGAAAG‑G‑CTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGT                                                                                           >  SRR4302545.7116274/1‑94 (MQ=60)
     TGAACGACGCCGAAATGCTGTCGATGG‑C‑G‑G‑GGAAAG‑G‑CTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCT‑Ggaagg                                                                                          >  SRR4302545.7998507/1‑89 (MQ=60)
     TGAACGACGCCGAAATGCTGTCGATGG‑C‑G‑G‑GGAAAG‑G‑CTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCT‑Ggaagg                                                                                          >  SRR4302545.3508853/1‑89 (MQ=60)
      GAACGACGCCGAAATGCTGTCGATGG‑C‑GTG‑G‑‑AAAGG‑CTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTG                                                                                          <  SRR4302545.3410799/94‑1 (MQ=60)
      GAACGACGCCGAAATGCTGTCGATGG‑C‑G‑GTG‑‑AAAGG‑CTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTG                                                                                          <  SRR4302545.4344462/94‑1 (MQ=60)
       AACGACGCCGAAATGCTGTCGATGG‑C‑G‑G‑GGAAAG‑G‑CTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGA                                                                                         <  SRR4302545.7829476/94‑1 (MQ=60)
       AACGACGCCGAAATGCTGTCGATGG‑C‑G‑G‑GGAAAG‑G‑CTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGA                                                                                         <  SRR4302545.3402111/94‑1 (MQ=60)
        ACGACGCCGAAATGCTGTCGATGG‑C‑G‑G‑GGAAAG‑G‑CTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGAT                                                                                        >  SRR4302545.5148732/1‑94 (MQ=60)
        ACGACGCCGAAATGCTGTCGATGG‑C‑G‑G‑GGAAAG‑G‑CTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGAT                                                                                        <  SRR4302545.3191078/94‑1 (MQ=60)
         TGACGCCGAAATGCTGTCGATGG‑C‑G‑G‑GGAAAG‑G‑CTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATT                                                                                       >  SRR4302545.9542392/1‑94 (MQ=60)
         CGACGCCGAAATGCTGTCGATGG‑C‑G‑G‑GGAAAG‑G‑CTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATT                                                                                       >  SRR4302545.9223589/1‑94 (MQ=60)
         CGACGCCGAAATGCTGTCGATGG‑C‑G‑G‑GGAAAG‑G‑CTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATT                                                                                       <  SRR4302545.6481843/94‑1 (MQ=60)
          GACGCCGAAATGCTGTCGATGG‑C‑G‑G‑GGAAAG‑G‑CTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGGAGTGATTG                                                                                      >  SRR4302545.8754024/1‑94 (MQ=60)
          GACGCCGAAATGCTGTCGATGG‑C‑G‑G‑GGAAAG‑G‑CTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTG                                                                                      >  SRR4302545.1236034/1‑94 (MQ=60)
          GACGCCGAAATGCTGTCGATGG‑C‑G‑G‑GGAAAG‑G‑CTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTG                                                                                      <  SRR4302545.6382791/94‑1 (MQ=60)
           ACGCCGAAATGCTGTCGATGG‑C‑G‑G‑GGAAAG‑G‑CTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGG                                                                                     >  SRR4302545.4178298/1‑94 (MQ=60)
           ACGCCGAAATGCTGTCGATGG‑C‑G‑G‑GGAAAG‑G‑CTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGG                                                                                     >  SRR4302545.448448/1‑94 (MQ=60)
           ACGCCGAAATGCTGTCGATGG‑C‑G‑G‑GGAAAG‑G‑CTGCATTATGGGCAATGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGG                                                                                     >  SRR4302545.2943880/1‑94 (MQ=60)
             GCCGAAATGCTGTCGATGG‑C‑G‑G‑GGAAAG‑G‑CTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTA                                                                                   <  SRR4302545.1231508/94‑1 (MQ=60)
             GCCGAAATGCTGTCGATGG‑C‑G‑G‑GGAAAG‑G‑CTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTA                                                                                   >  SRR4302545.1024512/1‑94 (MQ=60)
              CCGAAATGCTGTCGATGG‑C‑G‑G‑GGAAAG‑G‑CTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTAC                                                                                  <  SRR4302545.26158/94‑1 (MQ=60)
              CCGAAATGCTGTCGATGG‑C‑G‑G‑GGAAAG‑G‑CTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTAC                                                                                  >  SRR4302545.8977422/1‑94 (MQ=60)
               CGAAATGCTGTCGATGG‑C‑G‑G‑GGAAAG‑G‑CTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACT                                                                                 <  SRR4302545.10214141/94‑1 (MQ=60)
               CGAAATGCTGTCGATGG‑C‑G‑G‑GGAAAG‑G‑CTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACT                                                                                 >  SRR4302545.9302160/1‑94 (MQ=60)
               CGAAATGCTGTCGATGG‑C‑G‑G‑GGAAAG‑G‑CTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACT                                                                                 <  SRR4302545.8223082/94‑1 (MQ=60)
                GAAATGCTGTCGATGG‑C‑G‑G‑GGAAAG‑G‑CTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTA                                                                                >  SRR4302545.7185268/1‑94 (MQ=60)
                GAAATGCTGTCGATGG‑C‑G‑G‑GGAAAG‑G‑CTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTA                                                                                >  SRR4302545.9276335/1‑94 (MQ=60)
                 AAATGCTGTCGATGG‑C‑G‑G‑GGAAAG‑G‑CTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAA                                                                               >  SRR4302545.8450071/1‑94 (MQ=60)
                 AAATGCTGTCGATGG‑C‑G‑G‑GGAAAG‑G‑CTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAA                                                                               >  SRR4302545.5818790/1‑94 (MQ=60)
                  AATGCTGTCGATGG‑C‑G‑G‑GGAAAG‑G‑CTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAAT                                                                              <  SRR4302545.2152789/94‑1 (MQ=60)
                  AATGCTGTCGATGG‑C‑G‑G‑GGAAAG‑G‑CTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAAT                                                                              <  SRR4302545.8171036/94‑1 (MQ=60)
                  AATGCTGTCGATGG‑C‑G‑G‑GGAAAG‑G‑CTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAAT                                                                              >  SRR4302545.9351055/1‑94 (MQ=60)
                   ATGCTGTCGATGG‑C‑G‑G‑GGAAAG‑G‑CTGCATTATGGGCAGTGCGCACGAGGGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATG                                                                             >  SRR4302545.1138357/1‑94 (MQ=57)
                   ATGCTGTCGATGG‑C‑G‑G‑GGAAAG‑G‑CTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATG                                                                             <  SRR4302545.2094356/94‑1 (MQ=60)
                   ATGCTGTCGATGG‑C‑G‑G‑GGAAAG‑G‑CTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATG                                                                             >  SRR4302545.120670/1‑94 (MQ=60)
                   ATGCTGTCGATGG‑C‑G‑G‑GGAAAG‑G‑CTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATG                                                                             >  SRR4302545.626915/1‑94 (MQ=60)
                    TGCTGTCGATGG‑C‑G‑G‑GGAAAG‑G‑CTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGC                                                                            <  SRR4302545.10114647/94‑1 (MQ=60)
                     GCTGTCGATGG‑C‑G‑G‑GGAAAG‑G‑CTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCC                                                                           >  SRR4302545.8668197/1‑94 (MQ=60)
                      CTGTCGATGG‑CGG‑G‑G‑‑AAA‑GTCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCG                                                                          <  SRR4302545.790550/94‑1 (MQ=60)
                      CTGTCGATGG‑C‑G‑G‑GGAAAG‑G‑CTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGGCCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCG                                                                          <  SRR4302545.3672183/94‑1 (MQ=60)
                      CTGTCGATGG‑C‑G‑G‑GGAAAG‑G‑CTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCG                                                                          >  SRR4302545.2046563/1‑94 (MQ=60)
                          CGATGG‑C‑G‑G‑GGAAAG‑G‑CTGCATTCTGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGA                                                                      >  SRR4302545.6904983/1‑94 (MQ=60)
                          CGATGG‑C‑G‑G‑GGAAAG‑G‑CTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGA                                                                      >  SRR4302545.2449497/1‑94 (MQ=60)
                          CGATGG‑C‑G‑G‑GGAAAG‑G‑CTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGA                                                                      >  SRR4302545.1077245/1‑94 (MQ=60)
                           GATGGCC‑G‑G‑G‑‑AAAGG‑CTGCATTATGGGCAGTGCGCACCAGCTTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGAC                                                                     <  SRR4302545.5711045/94‑1 (MQ=60)
                           GATGG‑C‑G‑G‑GGAAAG‑G‑CTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGAC                                                                     >  SRR4302545.10475377/1‑94 (MQ=60)
                           GATGG‑C‑G‑G‑GGAAAG‑G‑CTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGAC                                                                     <  SRR4302545.6026996/94‑1 (MQ=60)
                           GATGG‑C‑G‑G‑GGAAAG‑G‑CTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGAC                                                                     <  SRR4302545.8952903/94‑1 (MQ=60)
                           GATGG‑C‑G‑G‑GGAAAG‑G‑CTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGAC                                                                     >  SRR4302545.7548251/1‑94 (MQ=60)
                            ATGG‑C‑G‑G‑GGAAAG‑G‑CTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACG                                                                    >  SRR4302545.3327655/1‑94 (MQ=60)
                            ATGG‑C‑G‑G‑GGAAAG‑G‑CTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACG                                                                    <  SRR4302545.1363881/94‑1 (MQ=60)
                            ATGG‑C‑G‑G‑GGAAAG‑G‑CTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACG                                                                    >  SRR4302545.7356632/1‑94 (MQ=60)
                            ATGG‑C‑G‑G‑GGAAAG‑G‑CTGCATTATGGGCAGTGCGCACCAACGTCTGAAAGACCTTCATCCCGCGCTGGAAGTGATTGGTACTAATGCCGACGACG                                                                    >  SRR4302545.10357812/1‑94 (MQ=60)
                             TGG‑C‑G‑G‑GGAAAG‑G‑CTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGC                                                                   >  SRR4302545.8586227/1‑94 (MQ=60)
                                 C‑G‑G‑G‑‑AAAGG‑CTGCATTATGGGAAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCCGTG                                                               <  SRR4302545.4790025/94‑1 (MQ=60)
                                   G‑G‑G‑‑AAAGG‑CTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGC                                                              >  SRR4302545.7409144/1‑94 (MQ=60)
                                   G‑G‑G‑‑AAAGG‑CTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGC                                                              >  SRR4302545.10863334/1‑94 (MQ=60)
                                     cggggaaagG‑CTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGT                                                                <  SRR4302545.7723462/85‑1 (MQ=60)
                                     cggggaaagG‑CTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGT                                                                >  SRR4302545.8490347/10‑94 (MQ=60)
                                     cggggaaagG‑CTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGT                                                                >  SRR4302545.4730325/10‑94 (MQ=60)
                                     cggggaaagG‑CTGCATTATGGGCAGAGCGCACCACCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCGGACGACGCGGC                                                                >  SRR4302545.10347376/10‑94 (MQ=60)
                                     G‑G‑‑AAAGG‑CTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCC                                                             >  SRR4302545.3179704/1‑94 (MQ=60)
                                     G‑G‑‑AAAGG‑CTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCC                                                             >  SRR4302545.4144015/1‑94 (MQ=60)
                                     G‑G‑‑AAAGG‑CTGCATTATGGCCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCC                                                             >  SRR4302545.8791522/1‑94 (MQ=60)
                                      ggggaaagG‑CTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTG                                                               >  SRR4302545.1806187/9‑94 (MQ=60)
                                       G‑‑AAAGG‑CTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCG                                                            <  SRR4302545.9223344/94‑1 (MQ=60)
                                       G‑‑AAAGG‑CTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCG                                                            >  SRR4302545.1094499/1‑94 (MQ=60)
                                          AAG‑G‑CTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCA                                                          <  SRR4302545.5114892/94‑1 (MQ=60)
                                          AAG‑G‑CTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCA                                                          <  SRR4302545.10023098/94‑1 (MQ=60)
                                           AG‑G‑CTGCATTATGGGCAGTGCGCGCCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCAT                                                         <  SRR4302545.9252651/94‑1 (MQ=60)
                                           AG‑G‑CTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCAT                                                         >  SRR4302545.8625324/1‑94 (MQ=60)
                                           AG‑G‑CTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCAT                                                         <  SRR4302545.4823597/94‑1 (MQ=60)
                                           AG‑G‑CTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCAT                                                         <  SRR4302545.1455840/94‑1 (MQ=60)
                                              G‑CTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTA                                                       <  SRR4302545.6115956/94‑1 (MQ=60)
                                                CTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTAT                                                      >  SRR4302545.8767959/1‑94 (MQ=60)
                                                 TGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATC                                                     >  SRR4302545.3840938/1‑94 (MQ=60)
                                                  TCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCT                                                    <  SRR4302545.9312607/94‑1 (MQ=60)
                                                  GCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCT                                                    <  SRR4302545.105385/94‑1 (MQ=60)
                                                  GCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCT                                                    >  SRR4302545.1947041/1‑94 (MQ=60)
                                                   CATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTG                                                   <  SRR4302545.4717313/94‑1 (MQ=60)
                                                   CATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTG                                                   <  SRR4302545.2604527/94‑1 (MQ=60)
                                                   CATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTG                                                   <  SRR4302545.5651231/94‑1 (MQ=60)
                                                   CATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTG                                                   <  SRR4302545.5934507/94‑1 (MQ=60)
                                                    ATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGC                                                  <  SRR4302545.676184/94‑1 (MQ=60)
                                                    ATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGC                                                  <  SRR4302545.10385575/94‑1 (MQ=60)
                                                    ATTATGGGCAGGGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGC                                                  <  SRR4302545.8486651/94‑1 (MQ=60)
                                                     TTATGGGCAGTTCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCG                                                 <  SRR4302545.7617048/94‑1 (MQ=60)
                                                     TTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCG                                                 >  SRR4302545.7643387/1‑94 (MQ=60)
                                                     TTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCG                                                 >  SRR4302545.985036/1‑94 (MQ=60)
                                                       ATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTA                                               >  SRR4302545.2497482/1‑94 (MQ=60)
                                                       ATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTA                                               >  SRR4302545.5761269/1‑94 (MQ=60)
                                                        TGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTAA                                              >  SRR4302545.4856541/1‑94 (MQ=60)
                                                        TGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTAA                                              >  SRR4302545.5744336/1‑94 (MQ=60)
                                                         GGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTAAA                                             >  SRR4302545.10591659/1‑94 (MQ=60)
                                                         GGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTAAA                                             <  SRR4302545.4316980/94‑1 (MQ=60)
                                                          ggcaggacggcCCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTAAAC                                            <  SRR4302545.10650091/83‑1 (MQ=60)
                                                          GGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTAAAC                                            <  SRR4302545.5204722/94‑1 (MQ=60)
                                                           GCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGCAAACT                                           >  SRR4302545.7202512/1‑94 (MQ=60)
                                                            CAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGGGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTAAACTC                                          >  SRR4302545.4106952/1‑94 (MQ=60)
                                                             AGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTAAACTCT                                         >  SRR4302545.3194673/1‑94 (MQ=60)
                                                              gtgcgCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTAAACTCTA                                        <  SRR4302545.4322847/89‑1 (MQ=60)
                                                              gtgcgCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTAAACTCTA                                        <  SRR4302545.2417887/89‑1 (MQ=60)
                                                              gtgcgCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTAAACTCTA                                        >  SRR4302545.7938242/6‑94 (MQ=60)
                                                              gtgcgCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTAAACTCTA                                        <  SRR4302545.7310382/89‑1 (MQ=60)
                                                                gcgCACCAGCGTCTTAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTAAACTCTATT                                      <  SRR4302545.5608959/91‑1 (MQ=60)
                                                                 cgCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTAAACTCTATTT                                     >  SRR4302545.4460178/3‑94 (MQ=60)
                                                                 cgCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTAAACTCTATTT                                     <  SRR4302545.8882705/92‑1 (MQ=60)
                                                                 cgCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTAAACTCTATTT                                     >  SRR4302545.10542402/3‑94 (MQ=60)
                                                                  GCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTAAACTCTATTTT                                    >  SRR4302545.7728827/1‑94 (MQ=60)
                                                                   CACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTAAACTCTATTTAT                                   <  SRR4302545.5832143/94‑1 (MQ=60)
                                                                   CACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTAAACTCTATTTAT                                   <  SRR4302545.4424299/94‑1 (MQ=60)
                                                                     CCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTAAACTCTATTTATCG                                 <  SRR4302545.3154161/94‑1 (MQ=60)
                                                                     CCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTAAACTCTATTTATCG                                 <  SRR4302545.3565864/94‑1 (MQ=60)
                                                                      CAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTAAACTCTATTTATCGT                                <  SRR4302545.7037304/94‑1 (MQ=60)
                                                                      CAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTAAACTCTATTTATCGT                                >  SRR4302545.926190/1‑94 (MQ=60)
                                                                      CAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTAAACTCTATTTATCGT                                >  SRR4302545.3861602/1‑94 (MQ=60)
                                                                      CAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTAAACTCTATTTATCGT                                <  SRR4302545.7539846/94‑1 (MQ=60)
                                                                      CAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTAAACTCTATTTATCGT                                >  SRR4302545.2788207/1‑94 (MQ=60)
                                                                       AGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTAAACTCTATTTATCGTA                               >  SRR4302545.8580302/1‑94 (MQ=60)
                                                                       AGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTCAGTAAACTCTATTTATCGTA                               >  SRR4302545.5355247/1‑94 (MQ=60)
                                                                        GCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTAAACTCTATTTATCGTAA                              <  SRR4302545.1379482/94‑1 (MQ=60)
                                                                         CGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTAAACTCTATTTATCGTAAT                             <  SRR4302545.10671362/94‑1 (MQ=60)
                                                                         CGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTAAACTCTATTTATCGTAAT                             >  SRR4302545.9370564/1‑94 (MQ=60)
                                                                         CGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTAAACTCTATTTATCGTAAT                             >  SRR4302545.3888982/1‑94 (MQ=60)
                                                                         CGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTAAACTCTATTTATCGTAAT                             <  SRR4302545.3891351/94‑1 (MQ=60)
                                                                         CGTCTGAAACACCTTCAGCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTAAACTCTATTTATCGTAAT                             <  SRR4302545.5167970/94‑1 (MQ=60)
                                                                          ctccGAAAGACCTTCATCCCGAGCTGGGAGTGATTGGTTCTAATGCCCGCGACGCGGTGCCGCATTATCTGCGTAAACTCTATTTATCGTAATC                            <  SRR4302545.3127441/90‑1 (MQ=60)
                                                                          GTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTAAACTCTATTTATCGTAATC                            >  SRR4302545.3132303/1‑94 (MQ=60)
                                                                          GTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTAAACTCTATTTATCGTAATC                            >  SRR4302545.2284858/1‑94 (MQ=60)
                                                                           TCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTAAACTCTATTTATCGTAATCG                           >  SRR4302545.3432821/1‑94 (MQ=60)
                                                                           TCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTAAACTCTATTTATCGTAATCG                           <  SRR4302545.4026471/94‑1 (MQ=60)
                                                                           TCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTAAACTCTATTTATCGTAATCG                           <  SRR4302545.9756371/94‑1 (MQ=60)
                                                                            CTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTAAACTCTATTTATCGTAATCGT                          <  SRR4302545.6143399/94‑1 (MQ=60)
                                                                            CTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTAAACTCTATTTATCGTAATCGT                          <  SRR4302545.6143400/94‑1 (MQ=60)
                                                                            CTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTAAACTCTATTTATCGTAATCGT                          >  SRR4302545.388362/1‑94 (MQ=60)
                                                                             TGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTAAACTCTATTTATCGTAATCGTT                         >  SRR4302545.8438599/1‑94 (MQ=60)
                                                                             TGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTAAACTCTATTTATCGTAATCGTT                         <  SRR4302545.9483883/94‑1 (MQ=60)
                                                                               AAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTAAACTCTATTTATCGTAATCGTTCT                       <  SRR4302545.182006/94‑1 (MQ=60)
                                                                                AAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTTCCGCATTATCTGCGTAAACTCTATTTATCGTAATCGTTCTT                      <  SRR4302545.3586690/94‑1 (MQ=60)
                                                                                AAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTAAACTCTATTTATCGTAATCGTTCTT                      >  SRR4302545.8361056/1‑94 (MQ=60)
                                                                                AAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTAAACTCTATTTATCGTAATCGTTCTT                      <  SRR4302545.9471867/94‑1 (MQ=60)
                                                                                AAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTAAACTCTATTTATCGTAATCGTTCTT                      >  SRR4302545.9081973/1‑94 (MQ=60)
                                                                                AAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTAAACTCTATTTATCGTAATCGTTCTT                      >  SRR4302545.2253795/1‑94 (MQ=60)
                                                                                 AGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTAAACTCTATTTATCGTAATCGTTCTTT                     <  SRR4302545.7262494/94‑1 (MQ=60)
                                                                                 AGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTAAACTCTATTTATCGTAATCGTTCTTT                     >  SRR4302545.2979377/1‑94 (MQ=60)
                                                                                 AGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTAAACTCTATTTATCGTAATCGTTCTTT                     >  SRR4302545.2979076/1‑94 (MQ=60)
                                                                                  GACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTAAACTCTATTTATCGTAATCGTTCTTTA                    <  SRR4302545.4047029/94‑1 (MQ=60)
                                                                                  GACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTAAACTCTATTTATCGTAATCGTTCTTTA                    >  SRR4302545.10852334/1‑94 (MQ=60)
                                                                                  GACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTAAACTCTATTTATCGTAATCGTTCTTTA                    <  SRR4302545.4674642/94‑1 (MQ=60)
                                                                                  GACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTAAACTCTATTTATCGTAATCGTTCTTTA                    <  SRR4302545.1076961/94‑1 (MQ=60)
                                                                                  GACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTAAACTCTATTTATCGTAATCGTTCTTTA                    >  SRR4302545.10924165/1‑94 (MQ=60)
                                                                                  GACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTAAACTCTATTTATCGTAATCGTTCTTTA                    <  SRR4302545.301709/94‑1 (MQ=60)
                                                                                   ACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTAAACTCTATTTATCGTAATCGTTCTTTAT                   <  SRR4302545.7093238/94‑1 (MQ=60)
                                                                                   ACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTAAACTCTATTTATCGTAATCGTTCTTTAT                   >  SRR4302545.5599184/1‑94 (MQ=60)
                                                                                    CCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTAAACTCTATTTATCGTAATCGTTCTTTATT                  <  SRR4302545.3223206/94‑1 (MQ=60)
                                                                                     CTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTAAACTCTATTTATCGTAATCGTTCTTTATTT                 <  SRR4302545.6699341/94‑1 (MQ=60)
                                                                                      TTAATCCCGAGCTGGAAGTGGTTGGGACTAATTCACACGACGCGGTGCCGCCTTATCTGCGTAAACTCTATTTATCGATAACGTTCTTTATTTC                >  SRR4302545.5628188/1‑94 (MQ=28)
                                                                                       TCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTAAACTCTATTTATCGTAATCGTTCTTTATTTGG               <  SRR4302545.6175621/94‑1 (MQ=60)
                                                                                        CATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTAAACTCTATTTATCGTAATCGTTCTTTATTTGGT              >  SRR4302545.5994181/1‑94 (MQ=60)
                                                                                        CATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTAAACTCTATTTATCGTAATCGTTCTTTATTTGGT              <  SRR4302545.5517574/94‑1 (MQ=60)
                                                                                        CATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTAAACTCTATTTATCGTAATCGTTCTTTATTTGGT              >  SRR4302545.6311441/1‑94 (MQ=60)
                                                                                         ATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTAAACTCTATGTATCGTACTCGTTCTTTACTTGGTC             >  SRR4302545.1455059/1‑94 (MQ=60)
                                                                                           CCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTAAACTCTATTTATCGTAATCGTTCTTTATTTGGTCAG           <  SRR4302545.5696477/94‑1 (MQ=60)
                                                                                           CCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTAAACTCTATTTATCGTAATCGTTCTTTATTTGGTCAG           <  SRR4302545.3731306/94‑1 (MQ=60)
                                                                                             CGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTAAACTCTATTTATCGTAATCGTTCTTTATTTGGTCAGTT         <  SRR4302545.479101/94‑1 (MQ=60)
                                                                                             CGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTAAACTCTATTTATCGTAATCGTTCTTTATTTGGTCAGTT         <  SRR4302545.5222974/94‑1 (MQ=60)
                                                                                             CGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTAAACTCTATTTATCGTAATCGTTCTTTATTTGGTCAGTT         >  SRR4302545.167972/1‑94 (MQ=60)
                                                                                             CGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTAAACTCTATTTATCGTAATCGTTCTTTATTTGGTCAGTT         >  SRR4302545.5503720/1‑94 (MQ=60)
                                                                                             CGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTAAACTCTATTTATCGTAATCGTTCTTTATTTGGTCAGTT         >  SRR4302545.5081666/1‑94 (MQ=60)
                                                                                              GAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTAAACTCTATTTATCGTAATCGTTCTTTATTTGGTCAGTTG        >  SRR4302545.1982845/1‑94 (MQ=60)
                                                                                               AGCTGGAAGCGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTAAACTCTATTTATCGTAATCGTTCTTTATTTGGTCAGTTGT       >  SRR4302545.6567619/1‑94 (MQ=60)
                                                                                                GCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTAAACTCTATTTATCGTAATCGTTCTTTATTTGGTCAGTTGTC      <  SRR4302545.6748268/94‑1 (MQ=60)
                                                                                                GCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTAAACTCTATTTATCGTAATCGTTCTTTATTTGGTCAGTTGTC      >  SRR4302545.2747972/1‑94 (MQ=60)
                                                                                                 ctgGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTAAACTCTATTTATCGTAATCGTTCTTTATTTGGTCAGTTGTCA     <  SRR4302545.6338435/91‑1 (MQ=60)
                                                                                                 ctgGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTAAACTCTATTTATCGTAATCGTTCTTTATTTGGTCAGTTGTCA     >  SRR4302545.130941/4‑94 (MQ=60)
                                                                                                 ctgGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTAAACTCTATTTATCGTAATCGTTCTTTATTTGGTCAGTTGTCA     <  SRR4302545.8221060/91‑1 (MQ=60)
                                                                                                 ctgGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTAAACTCTATTTATCGTAATCGTTCTTTATTTGGTCAGTTGTCA     >  SRR4302545.8824206/4‑94 (MQ=60)
                                                                                                  tgGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTAAACTCTATTTATCGTAATCGTTCTTTATTTGGTCAGTTGTCAA    >  SRR4302545.8612499/3‑94 (MQ=60)
                                                                                                  tgGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTAAACTCTATTTATCGTAATCGTTCTTTATTTGGTCAGTTGTCAA    >  SRR4302545.4286250/3‑94 (MQ=60)
                                                                                                  tgGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTAAACTCTATTTATCGTAATCGTTCTTTATTTGGTCAGTTGTCAA    >  SRR4302545.565118/3‑94 (MQ=60)
                                                                                                  tgGAAGTGATTCGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTAAACTCTATTTATCGTAATCGTTCTTTATTTGGTCAGTTGTCAA    <  SRR4302545.3096562/92‑1 (MQ=60)
                                                                                                  G‑GAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTAAACTCTATTTATCGTAATCGTTCTTTATTTGGTCAGTTGTCAAC   <  SRR4302545.1611910/94‑1 (MQ=60)
                                                                                                  G‑GAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTAAACTCTATTTATCGTAATCGTTCTTTATTTGGTCAGTTGTCAAC   <  SRR4302545.4982130/94‑1 (MQ=60)
                                                                                                    GAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTAAACTCTATTTATCGTAATCGTTCTTTATTTGGTCAGTTGTCAACC  <  SRR4302545.637790/94‑1 (MQ=60)
                                                                                                    |                                                                                             
CGGGATGAACGACGCCGAAATGCTGTCGATGG‑C‑G‑G‑G‑‑AAA‑G‑CTGCATTATGGGCAGTGGCCACCAGCGTCTGAAAGACCTTCATCCCGAGCT‑GAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTAAACTCTATTTATCGTAATCGTTCTTTATTTGGTCAGTTGTCAACC  >  NC_000913/4008434‑4008618

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 2 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑