Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I1 R1 647 270.1 21258240 98.4% 20918108 94.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 4,013,772 +C intergenic (‑53/‑246) ysgA ← / → udp hypothetical protein/uridine phosphorylase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,013,7701.C99.2% 2236.8 / 4.6 643intergenic (‑51/‑248)ysgA/udphypothetical protein/uridine phosphorylase
Reads supporting (aligned to +/- strand):  ref base . (1/4);  new base C (322/315);  total (324/319)
Fisher's exact test for biased strand distribution p-value = 2.14e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 697 total aligned reads displayed.

CGCGTCGTCCGGGGTCTGAACGATGGTCGAAGCGAGAGGC‑ATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCG‑TA‑CC‑TTATAAAAATGTTGCGCAATGTTAACTATAGTCAGCATGCAACAAATCACATTGCCTGAATCGGCTCATCTTTTATGCAGTCCTGCA  >  NC_000913/4013678‑4013859
                                                                                               |                                                                                          
 gcgTCGTCCGGGGTCTGAACGATGGTCGAAGCGAGAGGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCG‑TA‑c                                                                                           >  2:2793723/1‑94 (MQ=255)
 gcgTCGGCCGGGGTCTGAACGATGGTCGAAGCGAGAGGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCG‑TA‑c                                                                                           <  1:1710345/94‑1 (MQ=255)
  cgtcgtCCGGGGTCTGAACGATGGTCGAAGCGAGAGGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCG‑TA‑cc                                                                                          >  1:4818783/1‑94 (MQ=255)
      gtcCGGGGTCTGAACGATGGTCGAAGCGAGAGGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCG‑TA‑ccctta                                                                                      >  1:1183484/1‑90 (MQ=255)
      gtcCGGGGTCTGAACGATGGTCGAAGCGAGAGGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCG‑TA‑ccctta                                                                                      >  2:8452918/1‑90 (MQ=255)
        ccGGGGTCTGAACGATGGTCGAAGCGAGAGGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCG‑TACCC‑Ttata                                                                                    <  2:4489274/94‑1 (MQ=255)
        ccGGGGTCTGAACGATGGTCGAAGCGAGAGGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCG‑TACCC‑Ttata                                                                                    <  2:4338131/94‑1 (MQ=255)
        ccGGGGTATGAACGATGGTCGAAGCGAGAGGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCG‑TACCC‑Ttata                                                                                    <  2:3110112/94‑1 (MQ=255)
         cGGGGTCTGAACGATGGTCGAAGCGAGAGGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCG‑TACCC‑TTATaa                                                                                   >  2:1320255/1‑94 (MQ=255)
         cGGGGTCTGAACGATGGTCGAAGCGAGAGGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCG‑TACCC‑TTATaa                                                                                   >  2:1515359/1‑94 (MQ=255)
         cGGGGTCTGAACGATGGTCGAAGCGAGAGGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCG‑TACCC‑TTATaa                                                                                   >  2:3400759/1‑94 (MQ=255)
          ggggTCTGAACGATGGTCGAAGCGAGAGGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCG‑TACCC‑TTATaaa                                                                                  >  1:10238201/1‑94 (MQ=255)
             gTCTGAACGATGGTCGAAGCGAGAGGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCG‑TACCC‑TTATAAAAAt                                                                               <  2:1902202/94‑1 (MQ=255)
               cTGAACGATGGTCGAAGCGAGAGGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCG‑TACCC‑TTATAAAAATGt                                                                             >  1:3377880/1‑94 (MQ=255)
               cTGAACGATGGTCGAAGCGAGAGGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCG‑TACCC‑TTATAAAAATGt                                                                             >  1:2083060/1‑94 (MQ=255)
               cTGAACGATGGTCGAAGCGAGAGGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCG‑TACCC‑TTATAAAAATGt                                                                             >  2:954088/1‑94 (MQ=255)
                 gAACGATGGTCGAAGCGAGAGGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCG‑TACCC‑TTATAAAAATGTTg                                                                           <  2:6064589/94‑1 (MQ=255)
                 gAACGATGGTCGAAGCGAGAGGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCG‑TACCC‑TTATAAAAATGTTg                                                                           >  2:7970867/1‑94 (MQ=255)
                 gAACGATGGTCGAAGCGAGAGGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCG‑TACCC‑TTATAAAAATGTTg                                                                           <  1:10560975/94‑1 (MQ=255)
                  aaCGATGGTCGAAGCGAGAGGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCG‑TACCC‑TTATAAAAATGTTgc                                                                          <  1:863923/94‑1 (MQ=255)
                   aCGATGGTCGAAGCGAGAGGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCG‑TACCC‑TTATAAAAATGTTgcg                                                                         >  1:5772303/1‑94 (MQ=255)
                    cGATGGTCGAAGCGAGAGGCGATGCCGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCG‑TACCC‑TTATAAAAATGTTgcgc                                                                        >  1:5079162/1‑94 (MQ=255)
                     gATGGTCGAAGCGAGAGGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCG‑TACCC‑TTATAAAAATGTTGCGCa                                                                       >  2:1728885/1‑94 (MQ=255)
                      aTGGTCGAAGCGAGAGGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCG‑TACCC‑TTATAAAAATGTTGCGCaa                                                                      <  1:9508202/94‑1 (MQ=255)
                        ggTCGAAGCGAGAGGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCG‑TACCC‑TTATAAAAATGTTGCGCAATg                                                                    <  1:9341864/94‑1 (MQ=255)
                         gTCGAAGCGAGAGGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCG‑TACCC‑TTATAAAAATGTTGCGCAATGt                                                                   >  2:5500591/1‑94 (MQ=255)
                          tCGAAGCGAGAGGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCG‑TACCC‑TTATAAAAATGTTGCGCAATGtt                                                                  <  2:10369337/94‑1 (MQ=255)
                           cGAAGCGAGAGGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCG‑TACCC‑TTATAAAAATGTTGCGCAATGTTa                                                                 <  1:467323/94‑1 (MQ=255)
                           cGAAGCGAGAGGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCG‑TACCC‑TTATAAAAATGTTGCGCAATGTTa                                                                 >  1:7675870/1‑94 (MQ=255)
                            gAAGCGAGAGGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCG‑TACCC‑TTATAAAAATGTTGCGCAATGTTaa                                                                <  1:8365397/94‑1 (MQ=255)
                             aaGCGAGAGGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCG‑TACCC‑TTATAAAAATGTTGCGCAATGTTAAc                                                               <  2:9761070/94‑1 (MQ=255)
                              aGCGAGAGGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCG‑TACCC‑TTATAAAAATGTTGCGCAATGTTAACt                                                              >  2:524341/1‑94 (MQ=255)
                              aGCGAGAGGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCG‑TACCC‑TTATAAAAATGTTGCGCAATGTTAACt                                                              <  2:890852/94‑1 (MQ=255)
                              aGCGAGAGGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCG‑TACCC‑TTATAAAAATGTTGCGCAATGTTAACt                                                              <  1:1264977/94‑1 (MQ=255)
                                     ggcgaTGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCG‑TACCC‑TTATAAAAATGTTGCGCAATGTTAACTATAGTCa                                                       <  2:4792929/90‑1 (MQ=255)
                                     ggcgaTGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCG‑TACCC‑TTATAAAAATGTTGCGCAATGTTAACTATAGTCa                                                       <  2:3565083/90‑1 (MQ=255)
                                      gcgaTGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCG‑TACCC‑TTATAAAAATGTTGCGCAATGTTAACTATAGTCAg                                                      <  1:9870184/91‑1 (MQ=255)
                                       cgaTGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCG‑TACCC‑TTATAAAAATGTTGCGCAATGTTAACTATAGTCAGc                                                     <  2:9334459/92‑1 (MQ=255)
                                         aTGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCG‑TACCC‑TTATAAAAATGTTGCGCAATGTTAACTATAGTCAGCAt                                                   <  2:2566065/94‑1 (MQ=255)
                                         aTGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCG‑TACCC‑TTATAAAAATGTTGCGCAATGTTAACTATAGTCAGCAt                                                   <  2:8353750/94‑1 (MQ=255)
                                         aTGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCG‑TACCC‑TTATAAAAATGTTGCGCAATGTTAACTATAGTCAGCAt                                                   >  2:8740054/1‑94 (MQ=255)
                                             agcagGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCG‑TACCC‑TTATAAAAATGTTGCGCAATGTTAACTATAGTCAGCATGcaa                                               <  1:4419211/94‑1 (MQ=255)
                                              gcagGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCG‑TACCC‑TTATAAAAATGTTGCGCAATGTTAACTATAGTCAGCATGcaac                                              >  1:9634338/1‑94 (MQ=255)
                                                agGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCG‑TACCC‑TTATAAAAATGTTGCGCAATGTTAACTATAGTCAGCATGcaacaa                                            >  1:8277915/1‑94 (MQ=255)
                                                agGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCG‑TACCC‑TTATAAAAATGTTGCGCAATGTTAACTATAGTCAGCATGcaacaa                                            <  1:4190173/94‑1 (MQ=255)
                                                agGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCG‑TACCC‑TTATAAAAATGTTGCGCAATGTTAACTATAGTCAGCATGcaacaa                                            <  2:5042977/94‑1 (MQ=255)
                                                agGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCG‑TACCC‑TTATAAAAATGTTGAGCAATGTTAACTATAGTCAGCATGcaacaa                                            >  1:2717834/1‑94 (MQ=255)
                                                agGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCG‑TACCC‑TTATAAAAACGTTGCGCATTGTTAACTATAGTCAGCATGcaacaa                                            <  1:690415/94‑1 (MQ=255)
                                                 ggTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCG‑TACCC‑TTATAAAAATGTTGCGCAATGTTAACTATAGTCAGCATGCAACaaa                                           <  1:8570174/94‑1 (MQ=255)
                                                  gggCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCG‑TACCC‑TTATAAAAATGTTGCGCAATGTTAACTATAGTCAGCATGCAACAAAt                                          <  1:5205353/92‑1 (MQ=255)
                                                  gTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCG‑TACCC‑TTATAAAAATGTTGCGCAATGTTAACTATAGTCAGCATGCAACAAAt                                          >  1:7066716/1‑94 (MQ=255)
                                                   tGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCG‑TACCC‑TTATAAAAATGTTGCGCAATGTTAACTATAGTCAGCATGCAACAAATc                                         <  1:1948898/94‑1 (MQ=255)
                                                   tGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCG‑TACCC‑TTATAAAAATGTTGCGCAATGTTAACTATAGTCAGCATGCAACAAATc                                         <  1:74241/94‑1 (MQ=255)
                                                    gCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCG‑TACCC‑TTATAAAAATGTTGCGCAATGTTAACTATAGTCAGCATGCAACAAATca                                        >  2:1264387/1‑94 (MQ=255)
                                                    gCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCG‑TACCC‑TTATAAAAATGTTGCGCAATGTTAACTATAGTCAGCATGCAACAAATca                                        <  1:3681246/94‑1 (MQ=255)
                                                      aaaTCCAGGTTGTTGTGTTGTTGCCATGGTATTCTCCG‑TACCC‑TTATAAAAATGTTGCGCAATGTTAACTATAGTCAGCATGCAACAAATcaca                                      >  1:3225599/1‑94 (MQ=255)
                                                      aaaTCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCG‑TACCC‑TTATAAAAATGTTGCGCAATGTTAACTATAGTCAGCATGCAACAAATcaca                                      <  1:10382055/94‑1 (MQ=255)
                                                      aaaTCCAGATTGTTGTGTTGTTGCCATGGTATTCTACG‑TACCC‑TTATAAAAATGTTGCGCAATGTTAACTATAGTCAGCATGCAACAAATcaca                                      >  1:7237939/1‑94 (MQ=255)
                                                         tCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCG‑TACCC‑TTATAAAAATGTTGCGCAATGTTAACTATAGTCAGCATGCAACAAATCACATTg                                   <  1:6381335/94‑1 (MQ=255)
                                                          ccAGATTGTTGTGTTGTTGCCATGGTATTCTCCG‑TACCC‑TTATAAAAATGTTGCGCAATGTTAACTATAGTCAGCATGCAACAAATCACATTGc                                  >  2:3790352/1‑94 (MQ=255)
                                                           cAGATTGTTGTGTTGTTGCCATGGTATTCTCCG‑TACCC‑TTATAAAAATGTTGCGCAATGTTAACTATAGTCAGCATGCAACAAATCACATTGcc                                 >  2:5717833/1‑94 (MQ=255)
                                                             gATTGTTGTGTTGTTGCCATGGTATTCTCCG‑TACCC‑TTATAAAAATGTTGCGCAATGTTAACTATAGTCAGCATGCAACAAATCACATTGCCTg                               <  2:30061/94‑1 (MQ=255)
                                                             gATTGTTGTGTTGTTGCCATGGTATTCTCCG‑TACCC‑TTATAAAAATGTTGCGCAATGTTAACTATAGTCAGCATGCAACAAATCACATTGCCTg                               <  2:6894382/94‑1 (MQ=255)
                                                             gATTGTTGTGTTGTTGCCATGGTATTCTCCG‑TACCC‑TTATAAAAATGTTGCGCAATGTTAACTATAGTCAGCATGCAACAAATCACATTGCCTg                               <  1:9782136/94‑1 (MQ=255)
                                                              aTTGTTGTGTTGTTGCCATGGTATTCTCCG‑TACCC‑TTATAAAAATGTTGCGCAATGTTAACTATAGTCAGCATGCAACAAATCACATTGCCTGa                              >  2:5894148/1‑94 (MQ=255)
                                                                tgttgtgttgTTGCCATGGTATTCTCCG‑TACCC‑TTATAAAAATGTTGCGCAATGTTAACTATAGTCAGCATGCAACAAATCACATTGCCTGAAt                            <  1:8172996/94‑1 (MQ=255)
                                                                tgttgtgttgTTGCCATGGTATTCTCCG‑TACCC‑TTATAAAAATGTTGCGCAATGTTAACTATAGTCAGCATGCAACAAATCACATTGCCTGAAt                            <  1:4285318/94‑1 (MQ=255)
                                                                tgttgtgttgTTGCCATGGTATTCTCCG‑TACCC‑TTATAAAAATGTTGCGCAATGTTAACTATAGTCAGCATGCAACAAATCACATTGCCTGAAt                            <  2:10192237/94‑1 (MQ=255)
                                                                  ttgtgttgtTGCCATGGTATTCTCCG‑TACCC‑TTATAAAAATGTTGCGCAATGTTAACTATAGTCAGCATGCAACAAATCACATTGCCTGAATCg                          <  1:7769260/94‑1 (MQ=255)
                                                                  ttgtgttgtTGCCATGGTATTCTCCG‑TACCC‑TTATAAAAATGTTGCGCAATGTTAACTATAGTCAGCATGCAACAAATCACATTGCCTGAATCg                          <  1:2866826/94‑1 (MQ=255)
                                                                   tgtgttgtTGCCATGGTATTCTCCG‑TACCC‑TTATAAAAATGTTGCGCAATGTTAACTATAGTCAGCATGCAACAAATCACATTGCCTGAATCgg                         <  1:3489756/94‑1 (MQ=255)
                                                                   tgtgttgtTGCCATGGTATTCTCCG‑TACCC‑TTATAAAAATGTTGCGCAATGTTAACTATAGTCAGCATGCAACAAATCACATTGCCTGAATCgg                         >  2:9010314/1‑94 (MQ=255)
                                                                    gtgttgtTGCCATGGTATTCTCCG‑TACCC‑TTATAAAAATGTTGCGCAATGTTAACTATAGTCAGCATGCAACAAATCACATTGCCTGAATCGGc                        >  1:6703140/1‑94 (MQ=255)
                                                                    gtgttgtTGCCATGGTATTCTCCG‑TACCC‑TTATAAAAATGTTGCGCAATGTTAACTATAGTCAGCATGCAACAAATCACATTGCCTGAATCGGc                        >  1:9110330/1‑94 (MQ=255)
                                                                     tgttgtTGCCATGGTATTCTCCG‑TACCC‑TTATAAAAATGTTGCGCAATGTTAACTATAGTCAGCATGCAACAAATCACATTGCCTGAATCGGCt                       >  1:3073611/1‑94 (MQ=255)
                                                                     tgttgtTGCCATGGTATTCTCCG‑TACCC‑TTATAAAAATGTTGCGCAATGTTAACTATAGTCAGCATGCAACAAATCACATTGCCTGAATCGGCt                       >  2:6727239/1‑94 (MQ=255)
                                                                      gttgttGCCATGGTATTCTCCG‑TACCC‑TTATAAAAATGTTGCGCAATGTTAACTATAGTCAGCATGCAACAAATCACATTGCCTGAATCGGCTc                      <  1:2485787/94‑1 (MQ=255)
                                                                       ttgttgCCATGGTATTCTCCG‑TACCC‑TTATAAAAATGTTGCGCAATGTTAACTATAGTCAGCATGCAACAAATCACATTGCCTGAATCGGCTCa                     <  1:5390128/94‑1 (MQ=255)
                                                                          ttgCCATGGTATTCTCCG‑TACCC‑TTATAAAAATGTTGCGCAATGTTAACTATAGTCAGCATGCAACAAATCACATTGCCTGAATCGGCTCATCt                  <  2:10588383/94‑1 (MQ=255)
                                                                          ttgCCATGGTATTCTCCG‑TACCC‑TTATAAAAATGTTGCGCAATGTTAACTATAGTCAGCATGCAACAAATCACATTGCCTGAATCGGCTCATCt                  >  2:2301491/1‑94 (MQ=255)
                                                                           tgCCATGGTATTCTCCG‑TACCC‑TTATAAAAATGTTGCGCAATGTTAACTATAGTCAGCATGCAACAAATCACATTGCCTGAATCGGCTCATCtt                 >  1:7520773/1‑94 (MQ=255)
                                                                           tgCCATGGTATTCTCCG‑TACCC‑TTATAAAAATGTTGCGCAATGTTAACTATAGTCAGCATGCAACAAATCACATTGCCTGAATCGGCTCATCtt                 <  2:1127669/94‑1 (MQ=255)
                                                                            gCCATGGTATTCTCCG‑TACCC‑TTATAAAAATGTTGCGCAATGTTAACTATAGTCAGCATGCAACAAATCACATTGCCTGAATCGGCTCATCttt                >  1:995944/1‑94 (MQ=255)
                                                                             ccATGGTATTCTCCG‑TACCC‑TTATAAAAATGTTGCGCAATGTTAACTATAGTCAGCATGCAACAAATCACATTGCCTGAATCGGCTCATCtttt               >  2:2637726/1‑94 (MQ=255)
                                                                              cATGGTATTCTCCG‑TACCC‑TTATAAAAATGTTGCGCAATGTTAACTATAGTCAGCATGCAACAAATCACATTGCCTGAATCGGCTCATCTTTTa              <  2:8215645/94‑1 (MQ=255)
                                                                                tGGTATTCTCCG‑TACCC‑TTATAAAAATGTTGCGCAATGTTAACTATAGTCAGCATGCAACAAATCACATTGCCTGAATCGGCTCATCTTTTATg            <  1:8948230/94‑1 (MQ=255)
                                                                                 ggTATTCTCCG‑TACCC‑TTATAAAAATGTTGCGCAATGTTAACTATAGTCAGCATGCAACAAATCACATTGCCTGAATCGGCTCATCTTTTATGc           <  1:6115488/94‑1 (MQ=255)
                                                                                  gTATTCTCCG‑TACCC‑TTATAAAAATGTTGCGCAATGTTAACTATAGTCAGCATGCAACAAATCACATTGCCTGAATCGGCTCATCTTTTATGCa          >  1:7983960/1‑94 (MQ=255)
                                                                                  gTATGCTCCGTTC‑CC‑TTATAAAAATGTTGCGCAATGTTAACTATAGTCAGCATGCAACAAATCCCATTGCCTGAATCGGCTCATCTTTTATGCa          <  2:7323504/94‑1 (MQ=255)
                                                                                   tATTCTCCG‑TACCC‑TTATAAAAATGTTGCGCAATGTTAACTATAGTCAGCATGCAACAAATCACATTGCCTGAATCGGCTCATCTTTTATGCAg         <  2:412131/94‑1 (MQ=255)
                                                                                    aTTCTCCG‑TACCC‑TTATAAAAATGTTGCGCAATGTTAACTATAGTCAGCATGCAACAAATCACATTGCCTGAATCGGCTCATCTTTTATGCAGt        >  1:10001284/1‑94 (MQ=255)
                                                                                    aTTCTCCG‑TACCC‑TTATAAAAATGTTGCGCAATGTTAACTATAGTCAGCATGCAACAAATCACATTGCCTGAATCGGCTCATCTTTTATGCAGt        <  2:4886129/94‑1 (MQ=255)
                                                                                      tctcCG‑TACCC‑TTATAAAAATGTTGCGCAATGTTAACTATAGTCAGCATGCAACAAATCACATTGCCTGAATCGGCTCATCTTTTATGCAGTcc      >  2:714355/1‑94 (MQ=255)
                                                                                       ctcCG‑TACCC‑TTATAAAAATGTTGCGCAATGTTAACTATAGTCAGCATGCAACAAATCACATTGCCTGAATCGGCTCATCTTTTATGCAGTCCt     >  2:7738412/1‑94 (MQ=255)
                                                                                       ctcCG‑TACCC‑TTATAAAAATGTTGCGCAATGTTAACTATAGTCAGCATGCAACAAATCACATTGCCTGAATCGGCTCATCTTTTATGCAGTCCt     <  1:2369354/94‑1 (MQ=255)
                                                                                       ctcCG‑TACCC‑TTATAAAAATGTTGCGCAATGTTAACTATAGTCAGCATGCAACAAATCACATTGCCTGAATCGGCTCATCTTTTATGCAGTCCt     >  2:9186589/1‑94 (MQ=255)
                                                                                        tcCG‑TACCC‑TTATAAAAATGTTGCGCAATGTTAACTATAGTCAGCATGCAACAAATCACATTGCCTGAATCGGCTCATCTTTTATGCAGTCCTg    <  2:6443028/94‑1 (MQ=255)
                                                                                        tcCG‑TACCC‑TTATAAAAATGTTGCGCAATGTTAACTATAGTCAGCATGCAACAAATCACATTGCCTGAATCGGCTCATCTTTTATGCAGTCCTg    >  2:391953/1‑94 (MQ=255)
                                                                                         ccG‑TACCC‑TTATAAAAATGTTGCGCAATGTTAACTATAGTCAGCATGCAACAAATCACATTGCCTGAATCGGCTCATCTTTTATGCAGTCCTGc   >  1:4052813/1‑94 (MQ=255)
                                                                                          cG‑TA‑CCTTTATAAAAATGTTGCGCAATGTTAACTATAGTCAGCATGCAACAAATCACATTGCCTGAATCGGCTCATCTTTTATGCAGTCCTGCa  <  2:6630328/94‑1 (MQ=255)
                                                                                               |                                                                                          
CGCGTCGTCCGGGGTCTGAACGATGGTCGAAGCGAGAGGC‑ATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCG‑TA‑CC‑TTATAAAAATGTTGCGCAATGTTAACTATAGTCAGCATGCAACAAATCACATTGCCTGAATCGGCTCATCTTTTATGCAGTCCTGCA  >  NC_000913/4013678‑4013859

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output Only 200 of 343 total aligned reads displayed.
TGGCTGGTGGCACCGGCGACCTGACAGCGAAATTCTCCCGCCTGGTCGGAGAAACTGGCAAAGTGGTCCTTGCTGATATCAATGAATCCATGCCCAAAATGGGCCGCGAGAAGCTGCGTAATATCGGTGTGATTGGCAACGTTGAGTATGTTCAGGCGAACGCTGAGGCGCTGCCGTTCCCGGATAA  >  NC_000913/4016650‑4016836
                                                                                             |                                                                                             
TGGCTGGTGGCACCGGCGACCTGTCAGCGAAATTCTCCCGCCTGGTCGGAGAAACTGGCAAAGTGGTCCTTGCTGATATCAATGAATCCATGCT                                                                                               <  SRR4302545.5999312/94‑1 (MQ=60)
TGGCTGGTGGCACCGGCGACCTGACAGCGAAATTCTCCCGCCTGGTCGGAGAAACTGGCAAAGTGGTCCTTGCTGATATCAATGAATCCATGCT                                                                                               <  SRR4302545.1519845/94‑1 (MQ=60)
TGGCTGGTGGCACCGGCGACCTGACAGCGAAATTCTCCCGCCTGGTCGGAGAAACTGGCAAAGTGGTCCTTGCTGATATCAATGAATCCATGCT                                                                                               >  SRR4302545.8166622/1‑94 (MQ=60)
TGGCTGGGGGCGCGGGCGACCGGACAGCGAAATCCTCCCGCCTGGTCGGAGAAACTGGCAAAGTGGTCCTTGCTGATATCAATGAATCCATGCT                                                                                               <  SRR4302545.4020534/94‑1 (MQ=60)
  GCTGGTGGCACCGGCGACCTGACAGCGAAATTCTCCCGCCTGGTCGGAGAAACTGGCAAAGTGGTCCTTGCTGATATCAATGAATCCATGCTCA                                                                                             >  SRR4302545.70045/1‑94 (MQ=60)
    TGGTGGCACCGGCGACCTGACAGCGAAATTCTCCCGCCTGGTCGGAGAAACTGGCAAAGTGGTCCTTGCTGATATCAATGAATCCATGCTCAAA                                                                                           >  SRR4302545.6737844/1‑94 (MQ=60)
      GTGGCCCCGGGGGCCTGACAACCAAATTCTCCCGCCTGGTCGGAGAAACTGGCAAAGTGGTCCTTGCTGATATCAATGAATCCATGCTCAAAAT                                                                                         <  SRR4302545.1539143/94‑1 (MQ=60)
      GTGGCACCGGCGACCTGACAGCGAAATTCTCCCGCCTGGTCGGAGAAACTGGCAAAGTGGTCCTTGCTGATATCAATGAATCCATGCTCAAAAT                                                                                         >  SRR4302545.7199769/1‑94 (MQ=60)
      GTGGCACCGGCGACCTGACAGCGAAATTCTCCCGCCTGGTCGGAGAAACTGGCAAAGTGGTCCTTGCTGATATCAATGAATCCATGCTCAAAAT                                                                                         <  SRR4302545.2291502/94‑1 (MQ=60)
      GTGGCACCGGCGACCTGACAGCGAAATTCTCCCGCCTGGTCGGAGAAACTGGCAAAGTGGTCCTTGCTGATATCAATGAATCCATGCTCAAAAT                                                                                         <  SRR4302545.2681446/94‑1 (MQ=60)
      GTGGCACCGGCGACCTGACAGCGAAATTCTCCCGCCTGGTCGGAGAAACTGGCAAAGTGGTCCTTGCTGATATCAATGAATCCATGCTCAAAAT                                                                                         <  SRR4302545.3061433/94‑1 (MQ=60)
      GTGGCACCGGCGACCTGACAGCGAAATTCTCCCGCCTGGTCGGAGAAACTGGCAAAGTGGTCCTTGCTGATATCAATGAATCCATGCTCAAAAT                                                                                         <  SRR4302545.8888672/94‑1 (MQ=60)
      GTGGCACCGGCGACCTGACAGCGAAATTCTCCCGCCTGGTCGGAGAAACTGGCAAAGTGGTCCTTGCTGATATCAATGAATCCATGCTCAAAAT                                                                                         <  SRR4302545.4583437/94‑1 (MQ=60)
      GTGGCACCGGAGACCTGACAGCGAAATTCTCCCGCCTGGTCGGAGAAACTGGCAAAGTGGTCCTTGCTGATATCAATGAATCCATGCTCAAAAG                                                                                         >  SRR4302545.1879808/1‑94 (MQ=60)
       TGGCACCGGCGACCTGACAGCGAAATTCTCCCGCCTGGTCGGAGAAACTGGCAAAGTGGTCCTTGCTGATATCAATGAATCCATGCTCAAAATG                                                                                        <  SRR4302545.3923707/94‑1 (MQ=60)
       TGGCACCGGCGACCTGACAGCGAAATTCTCCCGCCTGGTCGGAGAAACTGGCAAAGTGGTCCTTGCTGATATCAATGAATCCATGCTCAAAATG                                                                                        <  SRR4302545.2205985/94‑1 (MQ=60)
       TGGCACCGGCGACCTGACAGCGAAATTCTCCCGCCTGGTCGGAGAAACTGGCAAAGTGGTCCTTGCTGATATCAATGAATCCATGCTCAAAATG                                                                                        >  SRR4302545.328381/1‑94 (MQ=60)
        GGCACCGGCGACCTGACAGCGAAATTCTCCCGCCTGGTCGGAGAAACTGGCAAAGTGGTCCTTGCTGATATCAATGAATCCATGCTCAAAATGG                                                                                       <  SRR4302545.6249682/94‑1 (MQ=60)
           ACCGGCGACCTGACAGCGAAATTCTCCCGCCTGGTCGGAGAAACTGGCAAAGTGGTCCTTGCTGATATCAATGAATCCATGCTCAAAATGGGCC                                                                                    <  SRR4302545.10544919/94‑1 (MQ=60)
           ACCGGCGACCTGACAGCGAAATTCTCCCGCCTGGTCGGAGAAACTGGCAAAGTGGTCCTTGCTGATATCAATGAATCCATGCTCAAAATGGGCC                                                                                    >  SRR4302545.4970071/1‑94 (MQ=60)
           ACCGGCGACCTGACAGCGAAATTCTCCCGCCTGGTCGGAGAAACTGGCAAAGTGGTCCTTGCTGATATCAATGAATCCATGCTCAAAATGGGCC                                                                                    >  SRR4302545.9517906/1‑94 (MQ=60)
           ACCGGCGACCTGACAGCGAAATTCTCCCGCCTGGTCGGAGAAACTGGCAAAGTGGTCCTTGCTGATATCAATGAATCCATGCTCAAAATGGGCC                                                                                    >  SRR4302545.9523924/1‑94 (MQ=60)
           ACAGGCGACCTGACAGCAAATTTCTCCCGCCTGGTCGGAGAAACTGGCAAAGTGGTCCTTGCTGATATCAATGAATCCATGCGCAAAATGGGCC                                                                                    <  SRR4302545.799406/94‑1 (MQ=60)
            CCGGCGACCTGACAGCGAAATTCTCCCGCCTGGTCGGAGAAACTGGCAAAGTGGTCCTTGCTGATATCAATGAATCCATGCTCAAAATGGGCCG                                                                                   >  SRR4302545.10513610/1‑94 (MQ=60)
            CCGGCGACCTGACAGCGAAATTCTCCCGCCTGGTCGGAGAAACTGGCAAAGTGGTCCTTGCTGATATCAATGAATCCATGCTCAAAATGGGCCG                                                                                   <  SRR4302545.830561/94‑1 (MQ=60)
             CGGCGACCTGACAGCGAAATTCTCCCGCCTGGTCGGAGAAACTGGCAAAGTGGTCCTTGCTGATATCAATGAATCCATGCTCAAAATGGGCCGC                                                                                  >  SRR4302545.3899397/1‑94 (MQ=60)
              GGCGACCTGACAGCGAAATTCTCCCGCCTGGTCGGAGAAACTGGCAAAGTGGTCCTTGCTGATATCAATGAATCCATGCTCAAAATGGGCCGCG                                                                                 >  SRR4302545.10882533/1‑94 (MQ=60)
               GCGACCTGACAGCGAAATTCTCCCGCCTGGTCGGAGAAACTGGCAAAGTGGTCTTTGCTGATATCAATGAATCCATGCTCAAAATGGGCCGCGA                                                                                <  SRR4302545.5657500/94‑1 (MQ=60)
                CGACCTGACAGCGAAATTCTCCCGCCTGGTCGGAGAAACTGGCAAAGTGGTCCTTGCTGATATCAATGAATCCATGCTCAAAATGGGCCGCGAG                                                                               <  SRR4302545.9794110/94‑1 (MQ=60)
                 GACCTGACAGCGAAATTCTCCCGCCTGGTCGGAGAAACTGGCAAAGTGGTCCTTGCTGATATCAATGAATCCATGCTCAAAATGGGCCGCGAGA                                                                              >  SRR4302545.688321/1‑94 (MQ=60)
                 GACCTGACAGCGAAATTCTCCCGCCTGGTCGGAGAAACTGGCAAAGTGGTCCTTGCTGATATCAATGAATCCATGCTCAAAATGGGCCGCGAGA                                                                              >  SRR4302545.6188609/1‑94 (MQ=60)
                  ACCTGACAGCGAAATTCTCCCGCCTGGTCGGAGAAACTGGCAAAGTGGTCCTTGCTGATATCAATGAATCCATGCTCAAAATGGGCCGCGAGAA                                                                             >  SRR4302545.6816491/1‑94 (MQ=60)
                  ACCTGACAGCGAAATTCTCCCGCCTGGTCGGAGAAACTGGCAAAGTGGTCCTTGCTGATATCAATGAATCCATGCTCAAAATGGGCCGCGAGAA                                                                             <  SRR4302545.752439/94‑1 (MQ=60)
                  ACCTGACAGCGAAATTCTCCCGCCTGGTCGGAGAAACTGGCAAAGTGGTCCTTGCTGATATCAATGAATCCATGCTCAAAATGGGCCGCGAGAA                                                                             >  SRR4302545.6400495/1‑94 (MQ=60)
                   CCTGACAGCGAAATTCTCCCGCCTGGTCGGAGAAACTGGCAAAGTGGTCCTTGCTGATATCAATGAATCCATGCTCAAAATGGGCCGCGAGAAG                                                                            <  SRR4302545.5532877/94‑1 (MQ=60)
                    CTGACAGCGAAATTCTCCCGCCTGGTCGGAGAAACTGGCAAAGTGGTCCTTGCTGATATCAATGAATCCATGCTCAAAATGGGCCGCGAGAAGC                                                                           >  SRR4302545.2834551/1‑94 (MQ=60)
                    CTGACAGCGAAATTCTCCCGCCTGGTCGGAGAAACTGGCAAAGTGGTCCTTGCTGATATCAATGAATCCATGCTCAAAATGGGCCGCGAGAAGC                                                                           <  SRR4302545.7237601/94‑1 (MQ=60)
                      GACAGCGAAATTCTCCCGCCTGGTCGGAGAAACTGGCAAAGTGGTCCTTGCTGATATCAATGAATCCATGCTCAAAATGGGCCGGGAGAAGCTG                                                                         >  SRR4302545.624568/1‑94 (MQ=60)
                      GACAGCGAAATTCTCCCGCCTGGTCGGAGAAACTGGCAAAGTGGTCCTTGCTGATATCAATGAATCCATGCTCAAAATGGGCCGCGAGAAGCTG                                                                         <  SRR4302545.6164370/94‑1 (MQ=60)
                       ACAGCGAAATTCTCCCGCCTGGTCGGAGAAACTGGCAAAGTGGTCCTTGCTGATATCAATGAATCCATGCTCAAAATGGGCCGCGAGAAGCTGC                                                                        <  SRR4302545.10292288/94‑1 (MQ=60)
                       ACAGCGAAATTCTCCCGCCTGGTCGGAGAAACTGGCAAAGTGGTCCTTGCTGATATCAATGAATCCATGCTCAAAATGGGCCGCGAGAAGCTGC                                                                        <  SRR4302545.9214044/94‑1 (MQ=60)
                       ACAGCGAAATTCTCCCGCCTGGTCGGAGAAACTGGCAAAGTGGTCCTTGCTGATATCAATGAATCCATGCTCAAAATGGGCCGCGAGAAGCTGC                                                                        >  SRR4302545.6776497/1‑94 (MQ=60)
                         AGCGAAATTCTCCCGCCTGGTCGGAGAAACTGGCAAAGTGGTCCTTGCTGATATCAATGAATCCATGCTCAAAATGGGCCGCGAGAAGCTGCGT                                                                      <  SRR4302545.5369782/94‑1 (MQ=60)
                         AGCGAAATTCTCCCGCCTGGTCGGAGAAACTGGCAAAGTGGTCCTTGCTGATATCAATGAATCCATGCTCAAAATGGGCCGCGAGAAGCTGCGT                                                                      <  SRR4302545.6579145/94‑1 (MQ=60)
                         AGCGAAATTCTCCCGCCTGGTCGGAGAAACTGGCAAAGTGGTCCTTGCTGATATCAATGAATCCATGCTCAAAATGGGCCGCGAGAAGCTGCGT                                                                      <  SRR4302545.6459859/94‑1 (MQ=60)
                         AGCGAAATTCTCCCGCCTGCTCGGAGAAACTGACAAAGTGTTCCTTGCTGATATCAATGAATCCATGCTCAAAATGGGCCGCGAGAAGCTGCGT                                                                      <  SRR4302545.2601166/94‑1 (MQ=60)
                           CGAAATTCTCCCGCCTGGTCGGAGAAACTGGCAAAGTGGTCCTTGCTGATATCAATGAATCCATGCTCAAAATGGGCCGCGAGAAGCTGCGTAA                                                                    >  SRR4302545.2274750/1‑94 (MQ=60)
                           CGAAATTCTCCCGCCTGGTCGGAGAAACTGGCAAAGTGGTCCTTGCTGATATCAATGAATCCATGCTCAAAATGGGCCGCGAGAAGCTGCGTAA                                                                    >  SRR4302545.1910560/1‑94 (MQ=60)
                            TAAATACTCCCGCCTGGTCGGAGAACCTGGCAAAGTGGTCCTTGCTGATATCACTGAATCCATGCTCAAAATGGGCCGCGCGAAGATGCGTAAT                                                                   <  SRR4302545.10305351/94‑1 (MQ=60)
                            GAAATTCTCCCGCCTGGTCGGAGAAACTGGCAAAGTGGTCCTTGCTGATATCAATGAATCCATGCTCAAAATGGGCCGCGAGAAGCTGCGTAAT                                                                   >  SRR4302545.5636640/1‑94 (MQ=60)
                             AAATTCTCCCGCCTGGTCGGAGAAACTGGCAAAGTGGTCCTTGCTGATATCAATGAATCCATGCTCAAAATGGGCCGCGAGAAGCTGCGTAATA                                                                  <  SRR4302545.8818999/94‑1 (MQ=60)
                             AAATTCTCCCGCCTGGTCGGAGAAACTGGCAAAGTGGTCCTTGCTGATATCAATGAATCCATGCTCAAAATGGGCCGCGAGAAGCTGCGTAATA                                                                  <  SRR4302545.2973303/94‑1 (MQ=60)
                              AATTCTCCCGCCTGGTCGGAGAAACTGGCATAGTGGGCCTTGCTGATATCAATGAATCCATGCTCAACATGGGCCGCGAGAAGCTGCGTAATAT                                                                 >  SRR4302545.6094794/1‑94 (MQ=60)
                              AATTCTCCCGCCTGGTCGGAGAAACTGGCAAAGTGGTCCTTGCTGATATCAATGAATCCATGCTCAAAATGGGCCGCGAGAAGCTGCGTAATAT                                                                 <  SRR4302545.10935835/94‑1 (MQ=60)
                              AATTCTCCCGCCTGGTCGGAGAAACTGGCAAAGTGGTCCTTGCTGATATCAATGAATCCATGCTCAAAATGGGCCGCGAGAAGCTGCGTAATAT                                                                 <  SRR4302545.8461003/94‑1 (MQ=60)
                              AATTCTCCCGCCTGGTCGGAGAAACTGGCAAAGTGGTCCTTGCTGATATCAATGAATCCATGCTCAAAATGGGCCGCGAGAAGCTGCGTAATAT                                                                 <  SRR4302545.988373/94‑1 (MQ=60)
                              AATTCTCCCGCCTGGTCGGAGAAACTGGCAAAGTGGTCCTTGCTGATATCAATGAATCCATGCTCAAAATGGGCCGCGAGAAGCTGCGTAATAT                                                                 >  SRR4302545.4160403/1‑94 (MQ=60)
                               ATTCTCCCGCCTGGTCGGAGAAACTGGCAAAGTGGTCCTTGCTGATATCAATGAATCCATGCTCAAAATGGGCCGCGAGAAGCTGCGTAATATC                                                                <  SRR4302545.10624819/94‑1 (MQ=60)
                               ATTCTCCCGCCTGGTCGGAGAAACTGGCAAAGTGGTCCTTGCTGATATCAATGAATCCATGCTCAAAATGGGCCGCGAGAAGCTGCGTAATATC                                                                <  SRR4302545.3479627/94‑1 (MQ=60)
                                TTCTCCCGCCTGGTCGGAGAAACTGGCAAAGTGGTCCTTGCTGATATCAATGAATCCATGCTCAAAATGGGCCGCGAGAAGCTGCGTAATATCG                                                               <  SRR4302545.731451/94‑1 (MQ=60)
                                 TCTCCCGCCTGGTCGGAGAAACTGGCAAAGTGGTCCTTGCTGATATCAATGAATCCATGCTCAAAATGGGCCGCGAGAAGCTGCGTAATATCGG                                                              <  SRR4302545.1819434/94‑1 (MQ=60)
                                 TCTCCCGCCTGGTCGGAGAAACTGGCAAAGTGGTCCTTGCTGATATCAATGAATCCATGCTCAAAATGGGCCGCGAGAAGCTGCGTAATATCGG                                                              <  SRR4302545.10002939/94‑1 (MQ=60)
                                  CTCCCGCCTGGTCGGAGAAACTGGCAAAGTGGTCCTTGCTGATATCAATGAATCCATGCTCAAAATGGGCCGCGAGAAGCTGCGTAATATCGGT                                                             >  SRR4302545.8070147/1‑94 (MQ=60)
                                   TCCCGCCTGGTCGGAGAAACTGGCAAAGTGGTCCTTGCTGATATCAATGAATCCATGCTCAAAATGGGCCGCGAGAAGCTGCGTAATATCGGTG                                                            >  SRR4302545.2867059/1‑94 (MQ=60)
                                    CCCGCCTGGTCGGAGAAACTGGCAAAGTGGTCCTTGCTGATATCAATGAATCCATGCTCAAAATGGGCCGCGAGAAGCTGCGTAATATCGGTGT                                                           <  SRR4302545.687350/94‑1 (MQ=60)
                                      CGCCTGGTCTGAGAAACTAGCAAAGTGGTCCTTGCTGATATCAATGAATCCATGCTCAAAATGGGCCGCGAGAAGCTGCGTAATATCGGTGTGA                                                         <  SRR4302545.8959501/94‑1 (MQ=60)
                                      CGCCTGGTCGGAGAAACTGGCAAAGTGGTCCTTGCTGATATCAATGAATCCATGCTCAAAATGGGCCGCGAGAAGCTGCGTAATATCGGTGTGA                                                         >  SRR4302545.3115641/1‑94 (MQ=60)
                                      CGCCTGGTCGGAGAAACTGGCAAAGTGGTCCTTGCTGATATCAATGAATCCATGCTCAAAATGGGCCGCGAGAAGCTGCGTAATATCGGTGTGA                                                         <  SRR4302545.115623/94‑1 (MQ=60)
                                       GCCTGGTCGGAGAAACTGGCAAAGTGGTCCTTGCTGATATCAATGAATCCATGCTCAAAATGGGCCGCGAGAAGCTGCGTAATATCGGTGTGAT                                                        >  SRR4302545.6148467/1‑94 (MQ=60)
                                        CCTGGTCGGAGAAACTGGCAAAGTGGTCCTTGCTGATATCAATGAATCCATGCTCAAAATGGGCCGCGAGAAGCTGCGTAATATCGGTGTGATT                                                       <  SRR4302545.4453328/94‑1 (MQ=60)
                                        CCTGGTCGGAGAAACTGGCAAAGTGGTCCTTGCTGATATCAATGAATCCATGCTCAAAATGGGCCGCGAGAAGCTGCGTAATATCGGTGTGATT                                                       >  SRR4302545.5320180/1‑94 (MQ=60)
                                        CCTGGTCGGAGAAACTGGCAAAGTGGTCCTTGCTGATATCAATGAATCCATGCTCAAAATGGGCCGCGAGAAGCTGCGTAATATCGGTGTGATT                                                       <  SRR4302545.3023969/94‑1 (MQ=60)
                                        CCTGGTCGGAGAAACTGGCAAAGTGGTCCTTGCTGATATCAATGAATCCATGCTCAAAATGGGCCGCGAGAAGCTGCGTAATATCGGTGTGATT                                                       >  SRR4302545.5911886/1‑94 (MQ=60)
                                        CCTGGTCGGAGAAACTGGCAAAGTGGTCCTTGCTGATATCAATGAATCCATGCTCAAAATGGGCCGCGAGAAGCTGCGTAATATCGGTGTGATT                                                       <  SRR4302545.7939859/94‑1 (MQ=60)
                                        CCTGGTCGGAGAAACTGGCAAAGTGGTCATTGCTGATATCAATGAATCCATGCTCAAAATGTGCCGCGAGAAGCTGCGTAATATCGGTGTGATT                                                       >  SRR4302545.5320179/1‑94 (MQ=60)
                                         CTGGTCGGAGAAACTGGCAAAGTGGTCCTTGCTGATATCAATGAATCCATGCTCAAAATGGGCCGCGAGAAGCTGCGTAATATCGGTGTGATTG                                                      <  SRR4302545.3491504/94‑1 (MQ=60)
                                         CTGGTCGGAGAAACTGGCAAAGTGGTCCTTGCTGATATCAATGAATCCATGCTCAAAATGGGCCGCGAGAAGCTGCGTAATATCGGTGTGATTG                                                      >  SRR4302545.8015027/1‑94 (MQ=60)
                                         CTGGTCGGAGAAACTGGCAAAGTGGTCCTTGCTGATATCAATGAATCCATGCTCAAAATGGGCCGCGAGAAGCTGCGTAATATCGGTGTGATTG                                                      >  SRR4302545.1929692/1‑94 (MQ=60)
                                         CTGGTCGGAGAAACTGGCAAAGTGGTCCTTGCTGATATCAATGAATCCATGCTCAAAATGGGCCGCGAGAAGCTGCGTAATATCGGTGTGATTG                                                      <  SRR4302545.8244534/94‑1 (MQ=60)
                                         CTGGTCGGAGAAACTGGCAAAGTGGTCCTTGCTGATATCAATGAATCCATGCTCAAAATGGGCCGCGAGAAGCTGCGTAATATCGGTGTGATTG                                                      <  SRR4302545.5015239/94‑1 (MQ=60)
                                         CTGGTCGGAGAAACTGGCAAAGTGGTCCTTGCTGATATCAATGAATCCATGCTCAAAATGGGCCGCGAGAAGCTGCGTAATATCGGTGTGATTG                                                      >  SRR4302545.10839417/1‑94 (MQ=60)
                                         CTGGTCGGAGAAACTGGCAAAGTGGTCCTTGCTGATATCAATGAATCCATGCTCAAAATGGGCCGCGAGAAGCTGCGTAATATCGGTGTGATTG                                                      <  SRR4302545.7438720/94‑1 (MQ=60)
                                          TGGTCGGAGAAACTGGCAAAGTGGTCCTTGCTGATATCAATGAATCCATGCTCAAAATGGGCCGCGAGAAGCTGCGTAATATCGGTGTGATTGG                                                     >  SRR4302545.6495169/1‑94 (MQ=60)
                                           GGTCGGAGAAACTGGCAAAGTGGTCCTTGCTGATATCAATGAATCCATGCTCAAAATGGGCCGCGAGAAGCTGCGTAATATCGGTGTGATTGGC                                                    <  SRR4302545.8614139/94‑1 (MQ=60)
                                           GGTCGGAGAAACTGGCAAAGTGGTCCTTGCTGATATCAATGAATCCATGCTCAAAATGGGCCGCGAGAAGCTGCGTAATATCGGTGTGATTGGC                                                    <  SRR4302545.5561712/94‑1 (MQ=60)
                                           GGTCGGAGAAACTGGCAAAGTGGTCCTTGCTGATATCAATGAATCCATGCTCAAAATGGGCCGCGAGAAGCTGCGTAATATCGGTGTGATTGGC                                                    <  SRR4302545.1021398/94‑1 (MQ=60)
                                           GGTCGGAGAAACTGGCAAAGTGGTCCTTGCTGATATCAATGAATCCATGCTCAAAATGGGCCGCGAGAAGCTGCGTAATATCGGTGTGATTGGA                                                    >  SRR4302545.3565221/1‑94 (MQ=60)
                                            GTCGGAGAAACTGGCAAAGTGGTCCTTGCTGATATCAATGAATCCATGCTCAAAATGGGCCGCGAGAAGCTGCGTAATATCGGTGTGATTGGCA                                                   <  SRR4302545.10916420/94‑1 (MQ=60)
                                             TCGGAGAAACTGGCAAAGTGGTCCTTGCTGATATCAATGAATCCATGCTCAAAATGGGCCGCGAGAAGCTGCGTAATATCGGTGTGATTGGCAA                                                  <  SRR4302545.3725387/94‑1 (MQ=60)
                                              CGGAGAAACTGGCAAAGTGGTCCTTGCTGATATCAATGAATCCATGCTCAAAATGGGCCGCGAGAAGCTGCGTAATATCGGTGTGATTGGCAAC                                                 <  SRR4302545.10600094/94‑1 (MQ=60)
                                              CGGAGAAACTGGCAAAGTGGTCCTTGCTGATATCAATGAATCCATGCTCAAAATGGGCCGCGAGAAGCTGCGTAATATCGGTGTGATTGGCAAC                                                 <  SRR4302545.5173280/94‑1 (MQ=60)
                                               GGAGAAACTGGCAAAGTGGTCCTTGCTGATATCAATGAATCCATGCTCAAAATGGGCCGCGAGAAGCTGCGTAATATCGGTGTGATTGGCAACG                                                <  SRR4302545.4058567/94‑1 (MQ=60)
                                               GGAGAAACTGGCAAAGTGGTCCTTGCTGATATCAATGAATCCATGCTCAAAATGGGCCGCGAGAAGCTGCGTAATATCGGTGTGATTGGCAACG                                                <  SRR4302545.2201581/94‑1 (MQ=60)
                                                GAGAAACTGGCAAAGTGGTCCTTGCTGATATCAATGAATCCATGCTCAAAATGGGCCGCGAGAAGCTGCGTAATATCGGTGTGATTGGCAACGT                                               >  SRR4302545.8823987/1‑94 (MQ=60)
                                                 AGAAACTGGCAAAGTGGTCCTTGCTGATATCAATGAATCCATGCTCAAAATGGGCCGCGAGAAGCTGCGTAATATCGGTGTGATTGGCAACGTT                                              <  SRR4302545.9648571/94‑1 (MQ=60)
                                                 AGAAACTGGCAAAGTGGTCCTTGCTGATATCAATGAATCCATGCTCAAAATGGGCCGCGAGAAGCTGCGTAATATCGGTGTGATTGGCAACGTT                                              <  SRR4302545.3804271/94‑1 (MQ=60)
                                                  GAAACTGGCAAAGTGGTCCTTGCTGATATCAATGAATCCATGCTCAAAATGGGCCGCGAGAAGCTGCGTAATATCGGTGTGATTGGCAACGTTG                                             <  SRR4302545.2347854/94‑1 (MQ=60)
                                                   AAACTGGCAAAGTGGTCCTTGCTGATATCACTGAATCCCTGTTCCAAATGGGACGAGAGACGCTGCGTAATATGGGTGTGtgtggcagagttgg                                            >  SRR4302545.4183446/1‑80 (MQ=28)
                                                   AAACTGGCAAAGTGGTCCTTGCTGATATCAATGAATCCATGCTCAAAATGGGCCGCGAGAAGCTGCGTAATATCGGTGTGATTGGCAACGTTGA                                            <  SRR4302545.9061728/94‑1 (MQ=60)
                                                   AAACTGGCAAAGTGGTCCTTGCTGATATCAATGAATCCATGCTCAAAATGGGCCGCGAGAAGCTGCGTAATATCGGTGTGATTGGCAACGTTGA                                            <  SRR4302545.10729177/94‑1 (MQ=60)
                                                   AAACTGGCAAAGTGGTCCTTGCTGATATCAATGAATCCATGCTCAAAATGGGCCGCGAGAAGCTGCGTAATATCGGTGTGATTGGCAACGTTGA                                            >  SRR4302545.8504797/1‑94 (MQ=60)
                                                   AAACTGGCAAAGTGGTCCTTGCTGATATCAATGAATCCATGCTCAAAATGGGCCGCGAGAAGCTGCGTAATATCGGTGTGATTGGCAACGTTGA                                            >  SRR4302545.9565214/1‑94 (MQ=60)
                                                    AACTGGCAAAGTGGTCCTTGCTGATATCAATGAATCCATGCTCAAAATGGGCCGCGAGAAGCTGCGTAATATCGGTGTGATTGGCAACGTTGAG                                           <  SRR4302545.2800566/94‑1 (MQ=60)
                                                    AACTGGCAAAGTGGTCCTTGCTGATATCAATGAATCCATGCTCAAAATGGGCCGCGAGAAGCTGCGTAATATCGGTGTGATTGGCAACGTTGAG                                           <  SRR4302545.6098020/94‑1 (MQ=60)
                                                     ACTGGCAAAGTGGTCCTTGCTGATATCAATGAATCCATGCTCAAAATGGGCCGCGAGAAGCTGCGTAATATCGGTGTGATTGGCAACGTTGAGT                                          <  SRR4302545.5359935/94‑1 (MQ=60)
                                                      CTGGCAAAGTGGTCCTTGCTGATATCAATGAATCCATGCTCAAAATGGGCCGCGAGAAGCTGCGTAATATCGGTGTGATTGGCAACGTTGAGTA                                         >  SRR4302545.8138637/1‑94 (MQ=60)
                                                      CTGGCAAAGTGGTCCTTGCTGATATCAATGAATCCATGCTCAAAATGGGCCGCGAGAAGCTGCGTAATATCGGTGTGATTGGCAACGTTGAGTA                                         >  SRR4302545.8237166/1‑94 (MQ=60)
                                                       TGGCAAAGTGGTCCTTGCTGATATCAATGAATCCATGCTCAAAATGGGCCGCGAGAAGCTGCGTAATATCGGTGTGATTGGCAACGTTGAGTAT                                        <  SRR4302545.958201/94‑1 (MQ=60)
                                                       TGGCAAAGTGGTCCTTGCTGATATCAATGAATCCATGCTCAAAATGGGCCGCGAGAAGCTGCGTAATATCGGTGTGATTGGCAACGTTGAGTAT                                        <  SRR4302545.9169145/94‑1 (MQ=60)
                                                        GGCAAAGTGGTCCTTGCTGATATCAATGAATCCATGCTCAAAATGGGCCGCGAGAAGCTGCGTAATATCGGTGTGATTGGCAACGTTGAGTATG                                       <  SRR4302545.59276/94‑1 (MQ=60)
                                                         GCAAAGTGGTCCTTGCTGATATCAATGAATCCATGCTCAAAATGGGCCGCGAGAAGCTGCGTAATATCGGTGTGATTGGCAACGTTGAGTATGT                                      >  SRR4302545.1677149/1‑94 (MQ=60)
                                                         GCAAAGTGGTCCTTGCTGATATCAATGAATCCATGCTCAAAATGGGCCGCGAGAAGCTGCGTAATATCGGTGTGATTGGCAACGTTGAGTATGT                                      <  SRR4302545.249010/94‑1 (MQ=60)
                                                          GAAAGTGGTCCTTGCTGATATCAGTGAATCCATGCTCAAAATGGGCCGGGAGAAGCTGCGTAATATCGGTGTGATTGGCAACGTTGAGTATGTT                                     <  SRR4302545.9912275/94‑1 (MQ=60)
                                                          GAAAGTGGTCCTTGCTGATATCAATGAATCCATGCTCAAAATGGGCCGCGAGAAGCTGCGTAATATCGGTGTGATTGGCAACGTTGAGTATGTT                                     <  SRR4302545.3529494/94‑1 (MQ=60)
                                                          CAAAGTGGTCCTTGCTGATATCAATGAATCCATGCTCAAAATGGGCCGCGAGAAGCTGCGTAATATCGGTGTGATTGGCAACGTTGAGTATGTT                                     >  SRR4302545.9437075/1‑94 (MQ=60)
                                                           AAAGTGGTCCTTGCTGATATCAATGAATCCATGCTCAAAATGGGCCGCGAGAAGCTGCGTAATATCGGTGTGATTGGCAACGTTGAGTATGTTC                                    <  SRR4302545.5239737/94‑1 (MQ=60)
                                                           AAAGTGGTCCTTGCTGATATCAATGAATCCATGCTCAAAATGGGCCGCGAGAAGCTGCGTAATATCGGTGTGATTGGCAACGTTGAGTATGTTC                                    >  SRR4302545.7514404/1‑94 (MQ=60)
                                                            AAGTGGTCCTTGCTGATATCAATGAATCCATGCTCAAAATGGGCCGCGAGAAGCTGCGTAATATCGGTGTGATTGGCAACGTTGAGTATGTTCA                                   <  SRR4302545.4603046/94‑1 (MQ=60)
                                                            AAGTGGTCCTTGCTGATATCAATGAATCCATGCTCAAAATGGGCCGCGAGAAGCGGCGTAATATCGGTGTGATTGGCAACGTTGAGTATGTTCA                                   <  SRR4302545.10009901/94‑1 (MQ=60)
                                                            AAGTGGTCCTTGCTGACATCAATGAATCCATGCTCAAAATGGGCGGCGAGAAGCTACGTAATATCGGTGTGATTGGCAACGTCGAGTATGTTCA                                   <  SRR4302545.952536/94‑1 (MQ=60)
                                                             AGTGGTCCTTGCTGATATCAATGAATCCATGCTCAAAATGGGCCGCGAGAAGCTGCGTAATATCGGTGTGATTGGCAACGTTGAGTATGTTCAG                                  <  SRR4302545.4262205/94‑1 (MQ=60)
                                                             AGTGGTCCTTGCTGATATCAATGAATCCATGCTCAAAATGGGCCGCGAGAAGCTGCGTAATATCGGTGTGATTGGCAACGTTGAGTATGTTCAG                                  <  SRR4302545.2014897/94‑1 (MQ=60)
                                                             AGTGGTCCTTGCTGATATCAATGAATCCATGCTCAAAATGGGCCGCGAGAAGCTGCGTAATATCGGTGTGATTGGCAACGTTGAGTATGTTCAG                                  <  SRR4302545.5989451/94‑1 (MQ=60)
                                                             AGTGGTCCTTGCTGATATCAATGAATCCATGCTCAAAATGGGCCGCGAGAAGCTGCGTAATATCGGTGTGATTGGCAACGTTGAGTATGTTCAG                                  <  SRR4302545.5844046/94‑1 (MQ=60)
                                                             AGTGGTCCTTGCTGATATCAATGAATCCATGCTCAAAATGGGCCGCGAGAAGCTGCGTAATATCGGTGTGATTGGCAACGTTGAGTATGTTCAG                                  >  SRR4302545.8599901/1‑94 (MQ=60)
                                                             AGTGGTCCTTGCTGATATCAATGAATCCATGCTCAAAATGCGCCGCGAGAAGCTGCGTAATATCGGTGTGATTGGCAACGTTGAGTATGTTCAG                                  <  SRR4302545.4994035/94‑1 (MQ=60)
                                                              GTGGTCCTTGCTGATATCAATGAATCCATGCTCAAAATGGGCCGCGAGAAGCTGCGTAATATCGGTGTGATTGGCAACGTTGAGTATGTTCAGG                                 <  SRR4302545.5161335/94‑1 (MQ=60)
                                                              GTGGTCCTTGCTGATATCAATGAATCCATGCTCAAAATGGGCCGCGAGAAGCTGCGTAATATCGGTGTGATTGGCAACGTTGAGTATGTTCAGG                                 <  SRR4302545.10398872/94‑1 (MQ=60)
                                                               TGGTCCTTGCTGATATCAATGAATCCATGCTCAAAATGGGCCGCGAGAAGCTGCGTAATATCGGTGTGATTGGCAACGTTGAGTATGTTCAGGC                                >  SRR4302545.1873129/1‑94 (MQ=60)
                                                               TGGTCCTTGCTGATATCAATGAATCCATGCTCAAAATGGGCCGCGAGAAGCTGCGTAATATCGGTGTGATTGGCAACGTTGAGTATGTTCAGGC                                <  SRR4302545.3361472/94‑1 (MQ=60)
                                                                GGTCCTTGCTGATATCAATGAATCCATGCTCAAAATGGGCCGCGAGAAGCTGCGTAATATCGGTGTGATTGGCAACGTTGAGTATGTTCAGGCG                               <  SRR4302545.2189357/94‑1 (MQ=60)
                                                                GGTCCTTGCTGATATCAATGAATCCATGCTCAAAATGGGCCGCGAGAAGCTGCGTAATATCGGTGTGATTGGCAACGTTGAGTATGTTCAGGCG                               <  SRR4302545.9714137/94‑1 (MQ=60)
                                                                GGTCCTTGCTGATATCAATGAATCCATGCTCAAAATGGGCCGCGAGAAGCTGCGTAATATCGGTGTGATTGGCAACGTTGAGTATGTTCAGGCG                               <  SRR4302545.2611258/94‑1 (MQ=60)
                                                                 GTCCTTGCTGATATCAATGAATCCATGCTCAAAATGGGCCGCGAGAAGCTGCGTAATATCGGTGTGATTGGCAACGTTGAGTATGTTCAGGCGA                              <  SRR4302545.10240254/94‑1 (MQ=60)
                                                                  TCCTTGCTGATATCAATGAATCCATGCTCAAAATGGGCCGCGAGAAGCTGCGTAATATCGGTGTGATTGGCAACGTTGAGTATGTTCAGGCGAA                             <  SRR4302545.8561734/94‑1 (MQ=60)
                                                                  TCCTTGCTGATATCAATGAATCCATGCTCAAAATGGGCCGCGAGAAGCTGCGTAATATCGGTGTGATTGGCAACGTTGAGTATGTTCAGGCGAA                             <  SRR4302545.1416282/94‑1 (MQ=60)
                                                                   CCTTGCTGATATCAATGAATCCATGCTCAAAATGGGCCGCGAGAAGCTGCGTAATATCGGTGTGATTGGCAACGTTGAGTATGTTCAGGCGAAC                            >  SRR4302545.10646213/1‑94 (MQ=60)
                                                                   CCTTGCTGATATCAATGAATCCATGCTCAAAATGGGCCGCGAGAAGCTGCGTAATATCGGTGTGATTGGCAACGTTGAGTATGTTCAGGCGAAC                            <  SRR4302545.1427284/94‑1 (MQ=60)
                                                                   CCTTGCTGATATCAATGAATCCATGCTCAAAATGGGCCGCGAGAAGCTGCGTAATATCGGTGTGATTGGCAACGTTGAGTATGTACAGGCGAAC                            <  SRR4302545.8928775/94‑1 (MQ=60)
                                                                    CTTGCTGATATCAATGAATCCATGCTCAAAATGGGCCGCGAGAAGCTGCGTAATATCGGTGTGATTGGCAACGTTGAGTATGTTCAGGCGAACG                           <  SRR4302545.4388952/94‑1 (MQ=60)
                                                                    CTTGCTGATATCAATGAATCCATGCTCAAAATGGGCCGCGAGAAGCTGCGTAATATCGGTGTGATTGGCAACGTTGAGTATGTTCAGGCGAACG                           <  SRR4302545.7595476/94‑1 (MQ=60)
                                                                    CTTGCTGATATCAATGAATCCATGCTCAAAATGGGCCGCGAGAAGCTGCGTAATATCGGTGTGATTGGCAACGTTGAGTATGTTCAGGCGAACG                           <  SRR4302545.1250700/94‑1 (MQ=60)
                                                                    CTTGCTGATATCAATGAATCCATGCTCAAAATGGGCCGCGAGAAGCTGCGTAATATCGGTGTGATTGGCAACGTTGAGTATGTTCAGGCGAACG                           >  SRR4302545.6382860/1‑94 (MQ=60)
                                                                     TTGCTGATATCAATGAATCCATGCTCAAAATGGGCCGCGAGAAGCTGCGTAATATCGGTGTGATTGGCAACGTTGAGTATGTTCAGGCGAACGC                          >  SRR4302545.5262516/1‑94 (MQ=60)
                                                                      TGCTGATATCAATGAATCCATGCTCAAAATGGGCCGCGAGAAGCTGCGTAATATCGGTGTGATTGGCAACGTTGAGTATGTTCAGGCGAACGCT                         <  SRR4302545.256959/94‑1 (MQ=60)
                                                                      TGCTGATATCAATGAATCCATGCTCAAAATGGGCCGCGAGAAGCTGCGTAATATCGGTGTGATTGGCAACGTTGAGTATGTTCAGGCGAACGCT                         >  SRR4302545.2702923/1‑94 (MQ=60)
                                                                      TGCTGATATCAATGAATCCATGCTCAAAATGGGCCGCGAGAAGCTGCGTAATATCGGTGTGATTGGCAACGTTGAGTATGTTCAGGCGAACGCT                         <  SRR4302545.6791821/94‑1 (MQ=60)
                                                                      TGCTGATATCAATGAATCCATGCTCAAAATGGGCCGCGAGAAGCTGCGTAATATCGGTGTGATTGGCAACGTTGAGTATGTTCAGGCGAACGCT                         >  SRR4302545.2152072/1‑94 (MQ=60)
                                                                       GCTGATATCAATGAATCCATGCTCAAAATGGGCCGCGAGAAGCTGCGTAATATCGGTGTGATTGGCAACGTTGAGTATGTTCAGGCGAAggcgg                        >  SRR4302545.1269439/1‑89 (MQ=60)
                                                                       GCTGATATCAATGAATCCATGCTCAAAATGGGCCGCGAGAAGCTGCGTAATATCGGTGTGATTGGCAACGTTGAGTATGTTCAGGCGAACGCTG                        <  SRR4302545.5460480/94‑1 (MQ=60)
                                                                        CTGATATCAATGAATCCATGCTCAAAATGGGCCGCGAGAAGCTGCGTAATATCGGTGTGATTGGCAACGTTGAGTATGTTCAGGCGAACGCTGA                       >  SRR4302545.5258928/1‑94 (MQ=60)
                                                                        CTGATATCAATGAATCCATGCTCAAAATGGGCCGCGAGAAGCTGCGTAATATCGGTGTGATTGGCAACGTTGAGTATGTTCAGGCGAACGCTGA                       <  SRR4302545.7659182/94‑1 (MQ=60)
                                                                        CTGATATCAATGAATCCATGCTCAAAATGGGCCGCGAGAAGCTGAGTAATATCGGTGTGATTGGCAACGTTGAGTATGTTCAGGCGAACGCTGA                       >  SRR4302545.9301448/1‑94 (MQ=60)
                                                                         TGATATCAATGAATCCATGCTCAAAATGGGCCGCGAGAAGCTGCGTAATATCGGTGTGATTGGCAACGTTGAGTATGTTCAGGCGCACGCTGAG                      >  SRR4302545.593607/1‑94 (MQ=60)
                                                                         TGATATCAATGAATACATGCTCAAAATGGGGCGCGAGAAGCTGCGTAATATCGGTGTGATTGGCATGGTTGGGAATGTTCTGGCGAACGCTGAG                      >  SRR4302545.5100430/1‑94 (MQ=60)
                                                                          GATATCAATGAATCCATGCTCAAAATGGGCCGCGAGAAGCTGCGTAATATCGGTGTGATTGGCAACGTTGAGTATGTTCAGGCGAACGCTGAGG                     <  SRR4302545.385681/94‑1 (MQ=60)
                                                                          GATATCAATGAATCCATGCTCAAAATGGGCCGCGAGAAGCTGCGTAATATCGGTGTGATTGGCAACGTTGAGTATGTTCAGGCGAACGCTGAGG                     <  SRR4302545.1392179/94‑1 (MQ=60)
                                                                          GATATCAATGAATCCATGCTCAAAATGGGCCGCGAGAAGCTGCGTAATATCGGTGTGATTGGCAACGTTGAGTATGTTCAGGCGAACGCTGAGG                     >  SRR4302545.117318/1‑94 (MQ=60)
                                                                          GATATCAATGAATCCATGCTCAAAATGGGCCGCGAGAAGCTGCGTAATATCGGTGTGATTGGCAACGTTGAGTATGTTCAGGCGAACGCTGAGG                     >  SRR4302545.928822/1‑94 (MQ=60)
                                                                           ATATCAATGAATCCATGCTCAAAATGGGCCGCGAGAAGCTGCGTAATATCGGTGTGATTGGCAACGTTGAGTATGTTCAGGCGAACGCTGAGGC                    <  SRR4302545.4324512/94‑1 (MQ=60)
                                                                             ATCAATGAATCCATGCTCAAAATGGGCCGCGAGAAGCTGCGTAATATCGGTGTGATTGGCAACGTTGAGTATGTTCAGGCGAACGCTGAGGCGC                  >  SRR4302545.1847472/1‑94 (MQ=60)
                                                                             ATCAATGAATCCATGCTCAAAATGGGCCGCGAGAAGCTGCGTAATATCGGTGTGATTGGCAACGTTGAGTATGTTCAGGCGAACGCTGAGGCGC                  >  SRR4302545.3462863/1‑94 (MQ=60)
                                                                             ATCAATGAATCCATGCTCAAAATGGGCCGCGAGAAGCTGCGTAATATCGGTGTGATTGGCAACGTTGAGTATGTTCAGGCGAACGCTGAGGCGC                  >  SRR4302545.3235974/1‑94 (MQ=60)
                                                                              TCAATGAATCCATGCTCAAAATGGGCCGCGAGAAGCTGCGTAATATCGGTGTGATTGGCAACGTTGAGTATGTTCAGGCGAACGCTGAGGCGCT                 <  SRR4302545.1068869/94‑1 (MQ=60)
                                                                              TCAATGAATCCATGCTCAAAATGGGCCGCGAGAAGCTGCGTAATATCGGTGTGATTGGCAACGTTGAGTATGTTCAGGCGAACGCTGAGGCGCT                 <  SRR4302545.3388159/94‑1 (MQ=60)
                                                                              TCAATGAATCCATGCTCAAAATGGGCCGCGAGAAGCTGCGTAATATCGGTGTGATTGGCAACGTTGAGTATGTTCAGGCGAACGCTGAGGCGCT                 <  SRR4302545.7478519/94‑1 (MQ=60)
                                                                               CAATGAATCCATGCTCAAAATGGGCCGCGAGAAGCTGCGTAATATCGGTGTGATTGGCAACGTTGAGTATGTTCAGGCGAACGCTGAGGCGCTG                >  SRR4302545.5203757/1‑94 (MQ=60)
                                                                               CAATGAATCCATGCTCAAAATGGGCCGCGAGAAGCTGCGTAATATCGGTGTGATTGGCAACGTTGAGTATGTTCAGGCGAACGCTGAGGCGCTG                >  SRR4302545.9180806/1‑94 (MQ=60)
                                                                               CAATGAATCCATGCTCAAAATGGGCCGCGAGAAGCTGCGTAATATCGGTGTGATTGGCAACGTTGAGTATGTTCAGGCGAACGCTGAGGCGCTG                <  SRR4302545.9491187/94‑1 (MQ=60)
                                                                               CAATGAATCCATGCTCAAAATGGGCCGCGAGAAGCTGCGTAATATCGGTGTGATTGGCAACGTTGAGTATGTTCAGGCGAACGCTGAGGCGCTG                <  SRR4302545.8748336/94‑1 (MQ=60)
                                                                               CAATGAATCCATGCTCAAAATGGGCCGCGAGAAGCTGCGTAATATCGGTGTGATTGGCAACGTTGAGTATGTTCAGGCGAACGCCGAGGCGCTG                >  SRR4302545.5203759/1‑94 (MQ=60)
                                                                               CAATGAATCCATGCTCAAAATGGGCCGCGAGAAGCTGCGTAATATCGGTGGGATTGGCAACGTTGAGTATGTTCAGGCGAACGCTGAGGCGCTG                >  SRR4302545.877509/1‑94 (MQ=60)
                                                                                AATGAATCCATGCTCAAAATGGGCCGCGAGAAGCTGCGTAATATCGGTGTGATTGGCAACGTTGAGTATGTTCAGGCGAACGCTGAGGCGCTGC               <  SRR4302545.6614682/94‑1 (MQ=60)
                                                                                 ATGAATCCATGCTCAAAATGGGCCGCGAGAAGCTGCGTAATATCGGTGTGATTGGCAACGTTGAGTATGTTCAGGCGAACGCTGAGGCGCTGCG              >  SRR4302545.7345549/1‑94 (MQ=60)
                                                                                 ATGAATCCATGCTCAAAATGGGCCGCGAGAAGCTGCGTAATATCGGTGTGATTGGCAACGTTGAGTATGTTCAGGCGAACGCTGAGGCGCTGCG              >  SRR4302545.8328868/1‑94 (MQ=60)
                                                                                  TGAATCCATGCTCAAAATGGGCCGCGAGAAGCTGCGTAATATCGGTGTGATTGGCAACGTTGAGTATGTTCAGGCGAACGCTGAGGCGCTGCCG             <  SRR4302545.1367680/94‑1 (MQ=60)
                                                                                   GAATCCATGCTCAAAATGGGCCGCGAGAAGCTGCGTAATATCGGTGTGATTGGCAACGTTGAGTATGTTCAGGGGAACGCTGAGGCGCTGCCGT            >  SRR4302545.3202441/1‑94 (MQ=60)
                                                                                   GAATCCATGCTCAAAATGGGCCGCGAGAAGCTGCGTAATATCGGTGTGATTGGCAACGTTGAGTATGTTCAGGCGAACGCTGAGGCGCTGCCGT            >  SRR4302545.3005712/1‑94 (MQ=60)
                                                                                    AATCCATGCTCAAAATGGGCCGCGAGAAGCTGCTTAATATCGGTGTGATTGGCAACGTTGAGTATGTTCAGGCGAACGCTGAGGCGCTGCCGTT           <  SRR4302545.7617701/94‑1 (MQ=60)
                                                                                    AATCCATGCTCAAAATGGGCCGCGAGAAGCTGCGTAATATCGGTGTGATTGGCAACGTTGAGTATGTTCAGGCGAACGCTGAGGCGCTGCCGTT           >  SRR4302545.10257269/1‑94 (MQ=60)
                                                                                    AATCCATGCTCAAAATGGGCCGCGAGAAGCTGCGTAATATCGGTGTGATTGGCAACGTTGAGTATGTTCAGGCGAACGCTGAGGCGCTGCCGTT           <  SRR4302545.702786/94‑1 (MQ=60)
                                                                                    AATCCATGCTCAAAATGGGCCGCGAGAAGCTGCGTAATATCGGTGTGATTGGCAACGTTGAGTATGTTCAGGCGAACGCTGAGGCGCTGCCGTT           >  SRR4302545.429146/1‑94 (MQ=60)
                                                                                    AATCCATGCTCAAAATGGGCCGCGAGAAGCTGCGTAATATCGGTGTGATTGGCAACGTTGAGTATGTTCAGGCGAACGCTGAGGCGCTGCCGTT           <  SRR4302545.2761624/94‑1 (MQ=60)
                                                                                    AATCCATGCTCAAAAGGGGCGGCGAGAAGCTGCGTAATATCGGTGTGATTGGCAACGTTGAGTATGTTCAGGCGAACGCTGAGGCGCTGCCGTT           <  SRR4302545.6702668/94‑1 (MQ=60)
                                                                                     ATCCATGCTCAAAATGGGCCGCGAGAAGCTGCGTAATATCGGTGTGATTGGCAACGTTGAGTATGTTCAGGCGAACGCTGAGGCGCTGCCGTTC          <  SRR4302545.4757629/94‑1 (MQ=60)
                                                                                     ATCCATGCTCAAAATGGGCCGCGAGAAGCTGCGTAATATCGGTGTGATTGGCAACGTTGAGTATGTTCAGGCGAACGCTGAGGCGCTGCCGTTC          >  SRR4302545.10639476/1‑94 (MQ=60)
                                                                                       CCATGCTCAAAATGGGCCGCGAGAAGCTGCGTAATATCGGTGTGATTGGCAACGTTGAGTATGTTCAGGCGAACGCTGAGGCGCTGCCGTTCCC        >  SRR4302545.8522886/1‑94 (MQ=60)
                                                                                       CCATGCTCAAAATGGGCCGCGAGAAGCTGCGTAATATCGGTGTGATTGGCAACGTTGAGTATGTTCAGGCGAACGCTGAGGCGCTGCCGTTCCC        <  SRR4302545.8849720/94‑1 (MQ=60)
                                                                                       CCATGCTCAAAATGGGCCGCGAGAAGCTGCGTAATATCGGTGTGATTGGCAACGTTGAGTATGTTCAGGCGAACGCTGAGGCGCTGCCGTTCCC        <  SRR4302545.10066638/94‑1 (MQ=60)
                                                                                        CATGCTCAAAATGGGCCGCGAGAAGCTGCGTAATATCGGTGTGATTGGCAACGTTGAGTATGTTCAGGCGAACGCTGAGGCGCTGCCGTTCCCG       >  SRR4302545.9106698/1‑94 (MQ=60)
                                                                                        CATGCTCAAAATGGGCCGCGAGAAGCTGCGTAATATCGGTGTGATTGGCAACGTTGAGTATGTTCAGGCGAACGCTGAGGCGCTGCCGTTCCCG       <  SRR4302545.8492995/94‑1 (MQ=60)
                                                                                        CATGCTCAAAATGGGCCGCGAGAAGCTGCGTAATATCGGTGTGATTGGCAACGTTGAGTATGTTCAGGCGAACGCTGAGGCGCTGCCGTTCCCG       >  SRR4302545.2719942/1‑94 (MQ=60)
                                                                                         ATGCTCAAAATGGGCCGCGAGAAGCTGCGTAATATCGGTGTGATTGGCAACGTTGAGTATGTTCAGGCGAACGCTGAGGCGCTGCCGTTCCCGG      <  SRR4302545.6429350/94‑1 (MQ=60)
                                                                                         ATGCTCAAAATGGGCCGCGAGAAGCTGCGTAATATCGGTGTGATTGGCAACGTTGAGTATGTTCAGGCGAACGCTGAGGCGCTGCCGTTCCCGG      <  SRR4302545.8982954/94‑1 (MQ=60)
                                                                                          TGCTCAAAATGGGCCGCGAGAAGCTGCGTAATATCGGTGTGATTGGCAACGTTGAGTATGTTCAGGCGAACGCTGAGGCGCTGCCGTTCCCGGA     >  SRR4302545.899532/1‑94 (MQ=60)
                                                                                          TGCTCAAAATGGGCCGCGAGAAGCTGCGTAATATCGGTGTGATTGGCAACGTTGAGTATGTTCAGGCGAACGCTGAGGCGCTGCCGTTCCCGGA     >  SRR4302545.7395154/1‑94 (MQ=60)
                                                                                           GCTCAAAATGGGCCGCGAGAAGCTGCGTAATATCGGTGTGATTGGCAACGTTGAGTATGTTCAGGCGAACGCTGAGGCGCTGCCGTTCCCGGAT    <  SRR4302545.8089856/94‑1 (MQ=60)
                                                                                           GCTCAAAATGGGCCGCGAGAAGCTGCGTAATATCGGTGTGATTGGCAACGTTGAGTATGTTCAGGCGAACGCTGAGGCGCTGCCGTTCCCGGAT    <  SRR4302545.1021949/94‑1 (MQ=60)
                                                                                            CTCAAAATGGGCCGCGAGAAGCTGCGTAATGTCGGTGTGATTGGCAACGTTGAGTATGTTCAGGCGAACGCTGAGGCGCTGCCGTTCCCGAATA   <  SRR4302545.5747279/94‑1 (MQ=60)
                                                                                             TCAAAATGGGCCGCGAGAAGCTGCGTAATATCGGTGTGATTGGCAACGTTGAGTATGTTCAGGCGAACGCTGAGGCGCTGCCGTTCCCGGATAA  >  SRR4302545.9998487/1‑94 (MQ=60)
                                                                                             |                                                                                             
TGGCTGGTGGCACCGGCGACCTGACAGCGAAATTCTCCCGCCTGGTCGGAGAAACTGGCAAAGTGGTCCTTGCTGATATCAATGAATCCATGCCCAAAATGGGCCGCGAGAAGCTGCGTAATATCGGTGTGATTGGCAACGTTGAGTATGTTCAGGCGAACGCTGAGGCGCTGCCGTTCCCGGATAA  >  NC_000913/4016650‑4016836

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 2 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑