Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A1 F1 I1 R1	647	270.1	21258240	98.4%	20918108	94.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,185,074	A→T	Q716L (CAG→CTG)	rpoC →	RNA polymerase beta prime subunit

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,185,074	0	A	T	99.8%	2244.2 / ‑5.4	573	Q716L (CAG→CTG)	rpoC	RNA polymerase beta prime subunit
Reads supporting (aligned to +/- strand): ref base A (1/0); new base T (295/277); total (296/277)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.89e-01

Only 100 of 581 total aligned reads displayed.

TGGGCTGCGGCGAACGATCGTGTATCCAAAGCGATGATGGATAACCTGCAAACTGAAACCGTGATTAACCGTGACGGTCAGGAAGAGAAGCAGGTTTCCTTCAACAGCATCTACATGATGGCCGACTCCGGTGCGCGTGGTTCTGCGGCACAGATTCGTCAGCTTGCTGGTATGCGTGGTCT  >  NC_000913/4184983‑4185164

 gggCTGCGGCGAACGATCGTGTATCCAAAGCGATGATGGATAACCTGCAAACTGAAACCGTGATTAACCGTGACGGTCAGGAAGAGAAGCTGGt                                                                                         <  2:2057741/94‑1 (MQ=255)

  ggCTGCGGCGAACGATCGTGTATCCAAAGCGATGATGGATAACCTGCAAACTGAAACCGTGATTAACCGTGACGGTCAGGAAGAGAAGCTGGtt                                                                                        >  2:3083433/1‑94 (MQ=255)

   gCTGCGGCGAACGATCGTGTATCCAAAGCGATGATGGATAACCTGCAAACTGAAACCGTGATTAACCGTGACGGTCAGGAAGAGAAGCTGGttt                                                                                       <  2:1464140/94‑1 (MQ=255)

      gcggcgAACGATCGTGTATCCAAAGCGATGATGGATAACCTGCAAACTGAAACCGTGATTAACCGTGACGGTCAGGAAGAGAAGCTGGTTTCCt                                                                                    <  2:6330623/94‑1 (MQ=255)

      gcggcgAACGATCGTGTATCCAAAGCGATGATGGATAACCTGCAAACTGAAACCGTGATTAACCGTGACGGTCAGGAAGAGAAGCTGGTTTCCt                                                                                    <  2:8181351/94‑1 (MQ=255)

          cgAACGATCGTGTATCCAAAGCGATGATGGATAACCTGCAAACTGAAACCGTGATTAACCGTGACGGTCAGGAAGAGAAGCTGGTTTCCTTCaa                                                                                <  1:3305611/94‑1 (MQ=255)

           gAACGATCGTGTATCCAAAGCGATGATGGATAACCTGCAAACTGAAACCGTGATTAACCGTGACGGTCAGGAAGAGAAGCTGGTTTCCTTCAAc                                                                               >  2:8676161/1‑94 (MQ=255)

           gAACGATCGTGTATCCAAAGCGATGATGGATAACCTGCAAACTGAAACCGTGATTAACCGTGACGGTCAGGAAGAGAAGCTGGTTTCCTTCAAc                                                                               <  2:3585276/94‑1 (MQ=255)

            aaCGATCGTGTATCCAAAGCGATGATGGATAACCTGCAAACTGAAACCGTGATTAACCGTGACGGTCAGGAAGAGAAGCTGGTTTCCTTCAACa                                                                              <  1:6998144/94‑1 (MQ=255)

             aCGATCGTGTATCCAAAGCGATGATGGATAACCTGCAAACTGAAACCGTGATTAACCGTGACGGTCAGGAAGAGAAGCTGGTTTCCTTCAACAg                                                                             <  1:2103443/94‑1 (MQ=255)

             aCGATCGTGTATCCAAAGCGATGATGGATAACCTGCAAACTGAAACCGTGATTAACCGTGACGGTCAGGAAGAGAAGCTGGTTTCCTTCAACAg                                                                             >  1:4042259/1‑94 (MQ=255)

               gATCGTGTATCCAAAGCGATGATGGATAACCTGCAAACTGAAACCGTGATTAACCGTGACGGTCAGGAAGAGAAGCTGGTTTCCTTCAACAGCa                                                                           >  1:6724385/1‑94 (MQ=255)

               gATCGTGTATCCAAAGCGATGATGGATAACCTGCAAACTGAAACCGTGATTAACCGTGACGGTCAGGAAGAGAAGCTGGTTTCCTTCAACAGCa                                                                           <  1:7815240/94‑1 (MQ=255)

               gATCGTGTATCCAAAGCGATGATGGATAACCTGCAAACTGAAACCGTGATTAACCGTGACGGTCAGGAAGAGAAGCTGGTTTCCTTCAACAGCa                                                                           <  1:9793419/94‑1 (MQ=255)

                aTCGTGTATCCAAAGCGATGATGGATAACCTGCAAACTGAAACCGGGATTAACCGTGACGGTCAGGAAGAGAAGCTGGTTTCCTTAAACAGCAt                                                                          >  2:2955842/1‑94 (MQ=255)

                 tCGTGTATCCAAAGCGATGATGGATAACCTGCAAACTGAAACCGTGATTAACCGTGACGGTCAGGAAGAGAAGCTGGTTTCCTTCAACAGCATc                                                                         >  2:4571975/1‑94 (MQ=255)

                 tCGTGTATCCAAAGCGATGATGGATAACCTGCAAACTGAAACCGTGATTAACCGTGACGGTCAGGAAGAGAAGCTGGTTTCCTTCAACAGCATc                                                                         <  1:9431534/94‑1 (MQ=255)

                 tCGTGTATCCAAAGCGATGATGGATAACCTGCAAACTGAAACCGTGATTAACCGTGACGGTCAGGAAGAGAAGCTGGTTTCCTTCAACAGCATc                                                                         >  2:3238568/1‑94 (MQ=255)

                  cGTGTATCCAAAGCGATGATGGATAACCTGCAAACTGAAACCGTGATTAACCGTGACGGTCAGGAAGAGAAGCTGGTTTCCTTCAACAGCATCt                                                                        >  2:110154/1‑94 (MQ=255)

                   gtgtATGCAAAGCGATGATGGATAACCTGCAAACTGAAACCGTGATTAACCGTGACGGTCAGGAAGAGAAGCTGGTTTCCTTCAACAGCATCTa                                                                       >  1:10522801/1‑94 (MQ=255)

                   gtgtATCCAAAGCGATGATGGATAACCTGCAAACTGAAACCGTGATTAACCGTGACGGTCAGGAAGAGAAGCTGGTTTCCTTCAACAGCATCTa                                                                       >  2:5903023/1‑94 (MQ=255)

                   gtgtATCCAAAGCGATGATGGATAACCTGCAAACTGAAACCGTGATTAACCGTGACGGTCAGGAAGAGAAGCTGGTTTCCTTCAACAGCATCTa                                                                       <  1:4868105/94‑1 (MQ=255)

                    tgtATCCAAAGCGATGATGGATAACCTGCAAACTGAAACCGTGATTAACCGTGACGGTCAGGAAGAGAAGCTGGTTTCCTTCAACAGCATCTAc                                                                      <  1:3587423/94‑1 (MQ=255)

                    tgtATCCAAAGCGATGATGGATAACCTGCAAACTGAAACCGTGATTAACCGTGACGGTCAGGAAGAGAAGCTGGTTTCCTTCAACAGCATCTAc                                                                      >  1:3138046/1‑94 (MQ=255)

                    tgtATCCAAAGCGATGATGGATAACCTGCAAACTGAAACCGTGATTAACCGTGACGGTCAGGAAGAGAAGCTGGTTTCCTTCAACAACATCTAc                                                                      >  1:7502565/1‑94 (MQ=255)

                     gtATCCAAAGCGATGATGGATAACCTGCAAACTGAAACCGTGATTAACCGTGACGGTCAGGAAGAGAAGCTGGTTTCCTTCAACAGCATCTACa                                                                     <  2:4309322/94‑1 (MQ=255)

                     gtATCCAAAGCGATGATGGATAACCTGCAAACTGAAACCGTGATTAACCGTGACGGTCAGGAAGAGAAGCTGGTTTCCTTCAACAGCATCTACa                                                                     <  1:4304265/94‑1 (MQ=255)

                      tATCCAAAGCGATGATGGATAACCTGCAAACTGAAACCGTGATTAACCGTGACGGTCAGGAAGAGAAGCTGGTTTCCTTCAACAGCATCTACat                                                                    <  1:9110004/94‑1 (MQ=255)

                      tATCCAAAGCGATGATGGATAACCTGCAAACTGAAACCGTGATTAACCGTGACGGTCAGGAAGAGAAGCTGGTTTCCTTCAACAGCATCTACat                                                                    <  2:7671159/94‑1 (MQ=255)

                      tATCCAAAGCGATGATGGATAACCTGCAAACTGAAACCGTGATTAACCGTGACGGTCAGGAAGAGAAGCTGGTTTCCTTCAACAGCATCTACat                                                                    <  2:6068934/94‑1 (MQ=255)

                        tCCAAAGCGATGATGGATAACCTGCAAACTGAAACCGTGATTAACCGTGACGGTCAGGAAGAGAAGCTGGTTTCCTTCAACAGCATCTACatga                                                                  >  2:508457/1‑94 (MQ=255)

                          cAAAGCGATGATGGATAACCTGCAAACTGAAACCGTGATTAACCGTGACGGTCAGGAAGAGAAGCTGGTTTCCTTCAACAGCATCTACatgatg                                                                >  2:5639994/1‑94 (MQ=255)

                          cAAAGCGATGATGGATAACCTGCAAACTGAAACCGTGATTAACCGTGACGGTCAGGAAGAGAAGCTGGTTTCCTTCAACAGCATCTACatgatg                                                                <  2:9145094/94‑1 (MQ=255)

                           aaaGCGATGATGGATAACCTGCAAACTGAAACCGTGATTAACCGTGACGGTCAGGAAGAGAAGCTGGATTCCTTCAACAGCATCTACATGATgg                                                               >  2:4484552/1‑94 (MQ=255)

                                gatgatGGATAACCTGCAAACTGAAACCGTGATTAACCGTGACTGTCAGGAAGAGAAGCTGGTTTCCTTCAACAGCATCTACATGATGGCCGAc                                                          <  1:5317420/94‑1 (MQ=255)

                                gatgatGGATAACCTGCAAACTGAAACCGTGATTAACCGTGACGGTCAGGAAGAGAAGCTGGTTTCCTTCAACAGCATCTACATGATGGCCGAc                                                          <  2:10162742/94‑1 (MQ=255)

                                gatgatGGATAACCTGCAAACTGAAACCGTGATTAACCGTGACGGTCAGGAAGAGAAGCTGGTTTCCTTCAACAGCATCTACATGATGGCCGAc                                                          <  1:4612427/94‑1 (MQ=255)

                                   gatgGATAACCTGCAAACTGAAACCGTGATTAACCGTGACGGTCAGGAAGAGAAGCTGGTTTCCTTCAACAGCATCTACATGATGGCCGACTcc                                                       <  2:797488/94‑1 (MQ=255)

                                   gatgGATAACCTGCAAACTGAAACCGTGATTAACCGTGACGGTCAGGAAGAGAAGCTGGTTTCCTTCAACAGCATCTACATGATGGCCGACTcc                                                       <  2:4082412/94‑1 (MQ=255)

                                        aTAACCTGCAAACTGAAACCGTGATTAACCGTGACGGTCAGGAAGAGAAGCTGGTTTCCTTCAACAGCATCTACATGATGGCCGACTCCGGTgc                                                  <  2:539889/94‑1 (MQ=255)

                                        aTAACCTGCAAACTGAAACCGTGATTAACCGTGACGGTCAGGAAGAGAAGCTGGTTTCCTTCAACAGCATCTACATGATGGCCGACTCCGGTgc                                                  >  2:2567148/1‑94 (MQ=255)

                                          aaCCTGCAAACTGAAACCGTGATTAACCGTGACGGTCAGGAAGAGAAGCTGGTTTCCTTCAACAGCATCTACATGATGGCCGACTCCGGTgcgc                                                <  1:8205755/94‑1 (MQ=255)

                                          aaCCTGCAAACTGAAACCGTGATTAACCGTGACGGTCAGGAAGAGAAGCTGGTTTCCTTCAACAGCATCTACATGATGGCCGACTCCGGTgcgc                                                >  2:7116719/1‑94 (MQ=255)

                                             cTGCAAACTGAAACCGTGATTAACCGTGACGGTCAGGAAGAGAAGCTGGTTTCCTTCAACAGCATCTACATGATGGCCGACTCCGGTGCGCGTg                                             >  1:6088453/1‑94 (MQ=255)

                                             cTGCAAACTGAAACCGTGATTAACCGTGACGGTCAGGAAGAGAAGCTGGTTTCCTTCAACAGCATCTACATGATGGCCGACTCCGGTGCGCGTg                                             <  2:6567910/94‑1 (MQ=255)

                                               gCAAACTGAAACCGTGATTAACCGTGACGGTCAGGAAGAGAAGCTGGTTTCCTTCAACAGCATCTACATGATGGCCGACTCCGGTGCGCGTGGt                                           <  2:7499667/94‑1 (MQ=255)

                                                cAAACTGAAACCGTGATTAACCGTGACGGTCAGGAAGAGAAGCTGGTTTCCTTCAACAGCATCTACATGATGGCCGACTCCGGTGCGCGTGGtt                                          <  1:7201761/94‑1 (MQ=255)

                                                cAAACTGAAACCGTGATTAACCGTGACGGTCAGGAAGAGAAGCTGGTTTCCTTCAACAGCATCTACATGATGGCCGACTCCGGCGCGCGTGGtt                                          >  2:2164303/1‑94 (MQ=255)

                                                 aaaCTGAAACCGTGATTAACCGTGACGGTCAGGAAGAGAAGCTGGTTTCCTTCAACAGCATCTACATGATGGCCGACTCCGGTGCGCGTGGTTc                                         >  1:2605137/1‑94 (MQ=255)

                                                 aaaCTGAAACCGTGATTAACCGTGACGGTCAGGAAGAGAAGCTGGTTTCCTTCAACAGCATCTACATGATGGCCGACTCCGGTGCGCGTGGTTc                                         >  2:8840756/1‑94 (MQ=255)

                                                  aaCTGAAACCGTGATTAACCGTGACGGTCAGGAAGAGAAGCTGGTTTCCTTCAACAGCATCTACATGATGGCCGACTCCGGTGCGCGTGGTTCt                                        <  1:7348033/94‑1 (MQ=255)

                                                  aaCTGAAACCGTGATTAACCGTGACGGTCAGGAAGAGAAGCTGGTTTCCTTCAACAGCATCTACATGATGGCCGACTCCGGTGCGCGTGGTTCt                                        >  2:2372847/1‑94 (MQ=255)

                                                   aCTGAAACCGTGTTTAACCGTGACGGTCAGGAAGAGAAGCTGGTTTCCTTCAACAGCATCTACATGATGGCCGACTCCGGTGCGCGTGGTTCTg                                       >  2:10424574/1‑94 (MQ=255)

                                                   aCTGAAACCGTGATTAACCGTGACGGTCAGGAAGAGAAGCTGGTTTCCTTCAACAGCATCTACATGATGGCCGACTCCGGTGCGCGTGGTTCTg                                       >  1:832757/1‑94 (MQ=255)

                                                     tGAAACCGTGATTAACCGTGACGGTCAGGAAGAGAAGCTGGTTTCCTTCAACAGCATCTACATGATGGCCGACTCCGGTGCGCGTGGTTCTGCg                                     >  1:7899699/1‑94 (MQ=255)

                                                     tGAAACCGTGATTAACCGTGACGGTCAGGAAGAGAAGCTGGTTTCCTTCAACAGCATCTACATGATGGCCGACTCCGGTGCGCGTGGTTCTGCg                                     >  1:6575471/1‑94 (MQ=255)

                                                       aaaCCGTGATTAACCGTGACGGTCAGGAAGAGAAGCTGGTTTCCTTCAACAGCATCTACATGATGGCCGACTCCGGTGCGCGTGGTTCTGCGGc                                   >  1:7764932/1‑94 (MQ=255)

                                                        aaCCGTGATTAACCGTGACGGTCAGGAAGAGAAGCTGGTTTCCTTCAACAGCATCTACATGATGGCCGACTCCGGTGCGCGTGGTTCTGCGGca                                  >  2:1250946/1‑94 (MQ=255)

                                                        aaCCGTGATTAACCGTGACGGTCAGGAAGAGAAGCTGGTTTCCTTCAACAGCATCTACATGATGGCCGACTCCGGTGCGCGTGGTTCTGCGGca                                  >  2:3738156/1‑94 (MQ=255)

                                                          ccGTGATTAACCGTGACGGTCAGGAAGAGAAGCTGGTTTCCTTCAACAGCATCTACATGATGGCCGACTCCGGTGCGCGTGGTTCTGCGGcaca                                >  1:8424469/1‑94 (MQ=255)

                                                           cGTGATTAACCGTGACGGTCAGGAAGAGAAGCTGGTTTCCTTCAACAGCATCTACATGATGGCCGACTCCGGTGCGCGTGGTTCTGCGGCACAg                               <  1:9560358/94‑1 (MQ=255)

                                                           cGTGATTAACCGTGACGGTCAGGAAGAGAAGCTGGTTTCCTTCAACAGCATCTACATGATGGCCGACTCCGGTGCGCGTGGTTCTGCGGCACAg                               <  1:4416845/94‑1 (MQ=255)

                                                            gTGATTAACCGTGACGGCCAGGAAGAGAAGCTGGTTTCCTTCAACAGCATCTACATGATGGCCGACTCCGGTGCGCGTGGTTCTGCGGCACAGa                              >  1:10055083/1‑94 (MQ=255)

                                                             tGATTAACCGTGACGGTCAGGAAGAGAAGCTGGTTTCCTTCAACAGCATCTACATGATGGCCGACTCCGGTGCGCGTGGTTCTGCGGCACAGAt                             >  2:1807632/1‑94 (MQ=255)

                                                             tGATTAACCGTGACGGTCAGGAAGAGAAGCTGGTTTCCTTCAACAGCATCTACATGATGGCCGACTCCGGTGCGCGTGGTTCTGCGGCACAGAt                             <  2:8102460/94‑1 (MQ=255)

                                                              gATTAACCGTGACGGTCAGGAAGAGAAGCTGGTTTCCTTCAACAGCATCTACATGATGGCCGACTCCGGTGCGCGTGGTTCTGCGGCACAGAtt                            >  2:2737740/1‑94 (MQ=255)

                                                              gATTAACCGTGACGGTCAGGAAGAGAAGCTGGTTTCCTTCAACAGCATCTACATGATGGCCGACTCCGGTGCGCGTGGTTCTGCGGCACAGAtt                            >  1:9943909/1‑94 (MQ=255)

                                                              gATGAACCGTGACGGTCAGGAAGAGAGGCTGGTTTCCCCCAACAGCATCTACAAGATGGCCGACTCCGGTGCGCGTGGTTCTGCGGCACAGAtt                            <  1:3037619/94‑1 (MQ=255)

                                                               aTTAACCGTGACGGTCAGGAAGAGAAGCTGGTTTCCTTCAACAGCATCTACATGATGGCCGACTCCGCTGCGCGTGGTTCTGCGGCACAGATTc                           <  2:8428897/94‑1 (MQ=255)

                                                                ttAACCGTGACGGTCAGGAAGAGAAGCTGGTTTCCTTCAACAGCATCTACATGATGGCCGACTCCGGTGCGCGTGGTTCTGCGGCACAGATTCg                          <  1:10353924/94‑1 (MQ=255)

                                                                  aaCCGTGACGGTCAGGAAGAGAAGCTGGTTTCCTTCAACAGCATCTACATGATGGCCGACTCCGGTGCGCGTGGTTCTGCGGCACAGATTCGTc                        >  1:6391457/1‑94 (MQ=255)

                                                                  aaCCGTGACGGTCAGGAAGAGAAGCTGGTTTCCTTCAACAGCATCTACATGATGGCCGACTCCGGTGCGCGTGGTTCTGCGGCACAGATTCGTc                        <  1:8731421/94‑1 (MQ=255)

                                                                    ccGTGACGGTCAGGAAGAGAAGCTGGTTTCCTTCAACAGCATCTACATGATGGCCGACTCCGGTGCGCGTGGTTCTGCGGCACAGATTCGTCAg                      <  2:3954373/94‑1 (MQ=255)

                                                                    ccGTGACGGTCAGGAAGAGAAGCTGGTTTCCTTCAACAGCATCTACATGATGGCCGACTCCGGTGCGCGTGGTTCTGCGGCACAGATTCGTCAg                      <  1:4150695/94‑1 (MQ=255)

                                                                    ccGTGACGGTCAGGAAGAGAAGCTGGTTTCCTTCAACAGCATCTACATGATGGCCGACTCCGGTGCGCGTGGTTCTGCGGCACAGATTCGTCAg                      <  2:6741869/94‑1 (MQ=255)

                                                                    ccGTGACGGTCAGGAAGAGAAGCTGGTTTCCTTCAACAGCATCTACATGATGGCCGACTCCGGTGCGCGTGGTTCTGCGGCACAGATTCGTCAg                      >  2:9325009/1‑94 (MQ=255)

                                                                     cGTGACGGTCAGGAAGAGAAGCTGGTTTCCTTCCACAGCATCTACATGATGGCCGACTCCGGTGCGCGTGGTTCTGCGGCACAGATTCGTCAGc                     >  2:4271062/1‑94 (MQ=255)

                                                                     cGTGACGGTCAGGAAGAGAAGCTGGTTTCCTTCAACAGCATCTACATGATGGCCGACTCCGGTGCGCGTGGTTCTGCGGCACAGATTCGTCAGc                     >  2:5259324/1‑94 (MQ=255)

                                                                       tGACGGTCAGGAAGAGAAGCTGGTTTCCTTCAACAGCATCTACATGATGGCCGACTCCGGTGCGCGTGGTTCTGCGGCACAGATTCGTCAGCtt                   <  1:5979288/94‑1 (MQ=255)

                                                                         aCGGTCAGGAAGAGAAGCTGGTTTCCTTCAACAGCATCTACATGATGGCCGACTCCGGTGCGCGTGGTTCTGCGGCACAGATTCGTCAGCTTGc                 <  2:949394/94‑1 (MQ=255)

                                                                          cGGTCAGGAAGAGAAGCTGGTTTCCTTCAACAGCATCTACATGATGGCCGACTCCGGTGCGCGTGGTTCTGCGGCACAGATTCGTCAGCTTGCt                >  1:1356896/1‑94 (MQ=255)

                                                                          cGGTCAGGAAGAGAAGCTGGTTTCCTTCAACAGCATCTACATGATGGCCGACTCCGGTGCGCGTGGTTCTGCGGCACAGATTCGTCAGCTTGCt                >  1:2452378/1‑94 (MQ=255)

                                                                           ggTCAGGAAGAGAAGCTGGTTTCCTTCAACAGCATCTACATGATGGCCGACTCCGGTGCGCGTGGTTCTGCGGCACAGATTCGTCAGCTTGCTg               >  2:10278587/1‑94 (MQ=255)

                                                                           ggTCAGGAAGAGAAGCTGGTTTCCTTCAACAGCATCTACATGATGGCCGACTCCGGTGCGCGTGGTTCTGCGGCACAGATTCGTCAGCTTGCTg               <  1:235553/94‑1 (MQ=255)

                                                                            gTCAGGAAGAGAAGCTGGTTTCCTTCAACAGCATCTACATGATGGCCGACTCCGGTGCGCGTGGTTCTGCGGCACAGATTCGTCAGCTTGCTgg              <  2:6927113/94‑1 (MQ=255)

                                                                            gTCAGGAAGAGAAGCTGGTTTCCTTCAACAGCATCTACATGATGGCCGACTCCGGTGCGCGTGGTTCTGCGGCACAGATTCGTCAGCTTGCTgg              >  1:161929/1‑94 (MQ=255)

                                                                             tCAGGAAGAGAAGCTGGTTTCCTTCAACAGCATCTACATGATGGCCGACTCCGGTGCGCGTGGTTCTGCGGCACAGATTCGTCAGCTTGCTGGt             <  2:4891520/94‑1 (MQ=255)

                                                                               aGGAAGAGAAGCTGGTTTCCTTCAACAGCATCTACATGATGGCCGACTCCGGTGCGCGTGGTTCTGCGGCACAGATTCGTCAGCTTGCTGGTAt           <  2:1637884/94‑1 (MQ=255)

                                                                                ggAAGAGAAGCTTGTTTCGTTGAACAGCATCTACATGGTGGCCGACTCCGGTGCGCGTGGTTCTGCGGCACAGATTCGTCAGCTTGCTGGTATg          <  2:3429779/94‑1 (MQ=255)

                                                                                ggAAGAGAAGCTGGTTTCCTTCAACAGCATCTACATGATGGCCGACTCCGGTGCGCGTGGTTCTGCGGCACAGATTCGTCAGCTTGCTGGTATg          >  2:7832924/1‑94 (MQ=255)

                                                                                ggAAGAGAAGCTGGTTTCCTTCAACAGCATCTACATGATGGCCGACTCCGGTGCGCGTGGTTCTGCGGCACAGATTCGTCAGCTTGCTGGTATg          <  2:8250516/94‑1 (MQ=255)

                                                                                   agagAAGCTGGTTTCCTTCAACAGCATCTACATGATGGCCGACTCCGGTGCGCGTGGTTCTGCGGCACAGATTCGTCAGCTTGCTGGTATGCGt       >  2:9907108/1‑94 (MQ=255)

                                                                                   agagAAGCTGGTTTCCTTCAACAGCATCTACATGATGGCCGACTCCGGTGCGCGTGGTTCTGCGGCACAGATTCGTCAGCTTGCTGGTATGCGt       >  1:3890438/1‑94 (MQ=255)

                                                                                   agagAAGCTGGTTTCCTTCAACAGCATCTACATGATGGCCGACTCCGGTGCGCGTGGTTCTGCGGCACAGATTCGTCAGCTTGCTGGTATGCGt       <  1:5468656/94‑1 (MQ=255)

                                                                                     agaAGCTGGTTTCCTTCAACAGCATCTACATGATGGCCGACTCCGGTGCGCGTGGTTCTGCGGCACAGATTCGTCAGCTTGCTGGTATGCGTgg     <  1:1925066/94‑1 (MQ=255)

                                                                                     agaAGCTGGTTTCCTTCAACAGCATCTACATGATGGCCGACTCCGGTGCGCGTGGTTCTGCGGCACAGATTCGTCAGCTTGCTGGTATGCGTgg     >  2:4738123/1‑94 (MQ=255)

                                                                                     agaAGCTGGTTTCCTTCAACAGCATCTACATGATGGCCGACTCCGGTGCGCGTGGTTCTGCGGCACAGATGCGTCAGCTTGCTGGTATGCGTgg     >  1:5862200/1‑94 (MQ=255)

                                                                                      gaAGCTGGTTTCCTTCAACAGCATCTACATGATGGCCGACTCCGGTGCGCGTGGTTCTGCGGCACAGATTCGTCAGCTTGCTGGTATGCGTGGt    >  1:6838956/1‑94 (MQ=255)

                                                                                       aaGCGGGTTTCCTTCAACAGCATCTACATGATGGGCGACTCCGGTGCGCGTGGTTCTGCGGCACAGATTCGTCAGCTTGCTGGTATGCGTGGTc   <  1:7673453/94‑1 (MQ=255)

                                                                                        aGCTGGTTTCCTTCAACAGCATCTACATGATGGCCGACTCCGGTGCGCGTGGTTCTGCGGCACAGATTCGTCAGCTTGCTGGTATGCGTGGGCt  >  2:7213347/1‑94 (MQ=255)

TGGGCTGCGGCGAACGATCGTGTATCCAAAGCGATGATGGATAACCTGCAAACTGAAACCGTGATTAACCGTGACGGTCAGGAAGAGAAGCAGGTTTCCTTCAACAGCATCTACATGATGGCCGACTCCGGTGCGCGTGGTTCTGCGGCACAGATTCGTCAGCTTGCTGGTATGCGTGGTCT  >  NC_000913/4184983‑4185164

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 22 ≤` `ATCG/ATCG` `< 30 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

BRESEQ :: bam2aln output

GATCGTATAATTGAAAAATTAGATCACGGCTGGTGGGTCGTCAGCCATGAACAAAAATTATGGTTGCCGAAGGGAGAATTGCCATATGGCGAA‑CGGGCAAATTTCGATCTTGTGGGTCAGCGCGCACTACAAATCGGCGAATGGCAGGGGGAACCTGTTTGGTTAGTACAACAGCAGCGGCG  >  NC_000913/4194484‑4194665

GATCGTATAATTGAAAAATTAGATCACGGCTGGTGGGTCGTCAGCCATGAACAAAAATTATGGTTGCCGAAGGGAGAATTGCCATATGGCGAA‑G                                                                                          <  SRR4302545.3259406/94‑1 (MQ=60)

GATCGTATAATTGAAAAATTAGATCACGGCTGGTGGGTCGTCAGCCATGAACAAAAATTATGGTTGCCGAAGGGAGAATTGCCATATGGCGAA‑G                                                                                          >  SRR4302545.7078472/1‑94 (MQ=60)

GATCGTATAATTGAAAAATTAGATCACGGCTGGTGGGTCGTCAGCCATGAACAAAAATTATGGTTGCCGAAGGGAGAATTGCCATATGGCGAA‑G                                                                                          <  SRR4302545.9281151/94‑1 (MQ=60)

GATCGTATAATTGAAAAATTAGATCACGGCTGGTGGGTCGTCAGCCATGAACAAAAATTATGGTTGCCGAAGGGAGAATTGCCATATGGCGAA‑G                                                                                          >  SRR4302545.10741024/1‑94 (MQ=60)

   CGTATAATTGAAAAATTAGATCACGGCTGGTGGGTCGTCAGCCATGAACAAAAATTATGGTTGCCGAAGGGAGAATTGCCATATGGCGAAGCGG                                                                                        >  SRR4302545.6463879/1‑94 (MQ=60)

     TATAATTGAAAAATTAGATCACGGCTGGTGGGTCGTCAGCCATGAACAAAAATTATGGTTGCCGAAGGGAGAATTGCCATATGGCGAA‑GCGGCA                                                                                     <  SRR4302545.10202703/94‑1 (MQ=60)

     TATAATTGAAAAATTAGATCACGGCTGGTGGGTCGTCAGCCATGAACAAAAATTATGGTTGCCGAAGGGAGAATTGCCATATGGCGAA‑GCGGCA                                                                                     >  SRR4302545.8890119/1‑94 (MQ=60)

      ATAATTGAAAAATTAGATCACGGCTGGAGGGCTGTCAGCCATGAACAAAAATTATGGTTGCCGTAGGGAGAATTTCCATATGGCGAA‑GCGGCAA                                                                                    >  SRR4302545.2976622/1‑94 (MQ=60)

       TAATTGAAAAATTAGATCACGGCTGGTGGGTCGTCAGCCATGAACAAAAATTATGGTTGCCGAAGGGAGAATTGCCATATGGCGAA‑GCGGCAAA                                                                                   >  SRR4302545.9214829/1‑94 (MQ=60)

        AATTGAAAAATTAGATCACGGCTGGTGGGTCGTCAGCCATGAACAAAAATTATGGTTGCCGAAGGGAGAATTGCCATATGGCGAA‑GCGGCAAAT                                                                                  <  SRR4302545.378461/94‑1 (MQ=60)

        AATTGAAAAATTAGATCACGGCTGGTGGGTCGTCAGCCATGAACAAAAATTATGGTTGCCGAAGGGAGAATTGCCATATGGCGAA‑GCGGCAAAT                                                                                  <  SRR4302545.9499341/94‑1 (MQ=60)

         ATTGAAAAATTAGATCACGGCTGGTGGGTCGTCAGCCATGAACAAAAATTATGGTTGCCGAAGGGAGAATTGCCATATGGCGAA‑GCGGCAAATT                                                                                 <  SRR4302545.2751980/94‑1 (MQ=60)

         ATTGAAAAATTAGATCACGGCTGGTGGGTCGTCAGCCATGAACAAAAATTATGGTTGCCGAAGGGAGAATTGCCATATGGCGAA‑GCGGCAAATT                                                                                 <  SRR4302545.9901009/94‑1 (MQ=60)

         ATTGAAAAATTAGATCACGGCTGGTGGGTCGTCAGCCATGAACAAAAATTATGGTTGCCGAAGGGAGAATTGCCATATGGCGAA‑GCGGCAAATT                                                                                 >  SRR4302545.3948846/1‑94 (MQ=60)

          TTGAAAAATTAGATCACGGCTGGTGGGTCGTCAGCCATGAACAAAAATTATGGTTGCCGAAGGGAGAATTGCCATATGGCGAA‑GCGGCAAATTT                                                                                <  SRR4302545.2224541/94‑1 (MQ=60)

          TGGAAAAATTAGATCACGGCTGGTGGGTCGTCAGCCATGAACAAAAATTATGGTTGCCGAAGGGAGAATTGCCATATGGCGAA‑GCGGCAAATTT                                                                                <  SRR4302545.2532269/94‑1 (MQ=60)

           TGAAAAATTAGATCACGGCTGGTGGGTCGTCAGCCATGAACAAAAATTATGGTTGCCGAAGGGAGAATTGCCATATGGCGAA‑GCGGCAAATTTC                                                                               <  SRR4302545.5563007/94‑1 (MQ=60)

            GAAAAATTAGATCACGGCTGGTGGGTCGTCAGCCATGAACAAAAATTATGGTTGCCGAAGGGAGAATTGCCATATGGCGAA‑GCGGCAAATTTCG                                                                              <  SRR4302545.9205843/94‑1 (MQ=60)

            GAAAAATTAGATCACGGCTGGTGGGTCGTCAGCCATGAACAAAAATTATGGTTGCCGAAGGGAGAATTGCCATATGGCGAA‑GCGGCAAATTTCG                                                                              <  SRR4302545.10718626/94‑1 (MQ=60)

             AAAAATTAGATCACGGCTGGTGGGTCGTCAGCCATGAACAAAAATTATGGTTGCCGAAGGGAGAATTGCCATATGGCGAA‑GCGGCAAATTTCGA                                                                             <  SRR4302545.3727761/94‑1 (MQ=60)

              AAAATTAGATCACGGCTGGTGGGTCGTCAGCTATGAACAAAAATTATGGTTGCCGAAGGGAGAATTGCCATATGGCGAA‑GCGGCAAATTTCGAT                                                                            <  SRR4302545.7504436/94‑1 (MQ=60)

              AAAATTAGATCACGGCTGGTGGGTCGTCAGCCATGAACAAAAATTATGGTTGCCGAAGGGAGAATTGCCATATGGCGAA‑GCGGCAAATTTCGAT                                                                            <  SRR4302545.10319049/94‑1 (MQ=60)

              AAAATTAGATCACGGCTGGTGGGTCGTCAGCCATGAACAAAAATTATGGTTGCCGAAGGGAGAATTGCCATATGGCGAA‑GCGGCAAATTTCGAT                                                                            >  SRR4302545.10143643/1‑94 (MQ=60)

              AAAATTAGATCACGGCTGGTGGGTCGTCAGCCATGAACAAAAATTATGGTTGCCGAAGGGAGAATTGCCATATGGCGAA‑GCGGCAAATTTCGAT                                                                            <  SRR4302545.6979213/94‑1 (MQ=60)

              AAAATTAGATCACGGCTGGTGGGTCGTCAGCCATGAACAAAAATTATGGTTGCCGAAGGGAGAATTGCCATATGGCGAA‑GCGGCAAATTTCGAT                                                                            <  SRR4302545.4700607/94‑1 (MQ=60)

               AAATTAGATCACGGCTGGTGGGTCGTCAGCCATGAACAAAAATTATGGTTGCCGAAGGGAGAATTGCCATATGGCGAA‑GCGGCAAATTTCGATC                                                                           <  SRR4302545.986472/94‑1 (MQ=60)

               AAATTAGATCACGGCTGGTGGGTCGTCAGCCATGAACAAAAATTATGGTTGCCGAAGGGAGAATTGCCATATGGCGAA‑GCGGCAAATTTCGATC                                                                           <  SRR4302545.10895706/94‑1 (MQ=60)

                AATTAGATCACGGCTGGTGGGTCGTCAGCCATGAACAAAAATTATGGTTGCCGAAGGGAGAATTGCCATATGGCGAA‑GCGGCAAATTTCGATCT                                                                          <  SRR4302545.778978/94‑1 (MQ=60)

                AATTAGATCACGGCTGGTGGGTCGTCAGCCATGAACAAAAATTATGGTTGCCGAAGGGAGAATTGCCATATGGCGAA‑GCGGCAAATTTCGATCT                                                                          <  SRR4302545.7511086/94‑1 (MQ=60)

                AATTAGATCACGGCTGGTGGGTCGTCAGCCATGAACAAAAATTATGGTTGCCGAAGGGAGAATTGCCATATGGCGAA‑GCGGCAAATTTCGATCT                                                                          <  SRR4302545.7511085/94‑1 (MQ=60)

                 ATTAGATCACGGCTGGTGGGTCGTCAGCCATGAACAAAAATTATGGTTGCCGAAGGGAGAATTGCCATATGGCGAA‑GCGGCAAATTTCGATCTT                                                                         <  SRR4302545.5112256/94‑1 (MQ=60)

                 ATTAGATCACGGCTGGTGGGTCGTCAGCCATGAACAAAAATTATGGTTGCCGAAGGGAGAATTGCCATATGGCGAA‑GCGGCAAATTTCGATCTT                                                                         <  SRR4302545.5112255/94‑1 (MQ=60)

                 ATTAGATCACGGCTGGTGGGTCGTCAGCCATGAACAAAAATTATGGTTGCCGAAGGGAGAATTGCCATATGGCGAA‑GCGGCAAATTTCGATCTT                                                                         <  SRR4302545.5051487/94‑1 (MQ=60)

                 ATTAGATCACGGCTGGTGGGTCGTCAGCCATGAACAAAAATTATGGTTGCCGAAGGGAGAATTGCCATATGGCGAA‑GCGGCAAATTTCGATCTT                                                                         <  SRR4302545.4315375/94‑1 (MQ=60)

                 ATTAGATCACGGCTGGTGGGTCGTCAGCCATGAACAAAAATTATGGTTGCCGAAGGGAGAATTGCCATATGGCGAA‑GCGGCAAATTTCGATCTT                                                                         <  SRR4302545.3423547/94‑1 (MQ=60)

                 ATTAGATCACGGCTGGTGGGTCGTCAGCCATGAACAAAAATTATGGTTGCCGAAGGGAGAATTGCCATATGGCGAA‑GCGGCAAATTTCGATCTT                                                                         <  SRR4302545.975748/94‑1 (MQ=60)

                 ATTAGATCACGGCTGGTGGGTCGTCAGCCATGAACAAAAATTATGGTTGCCGAAGGGAGAATTGCCATATGGCGAA‑GCGGCAAATTTCGATCTT                                                                         <  SRR4302545.8928782/94‑1 (MQ=60)

                 ATTAGATCACGGCTGGTGGGTCGTCAGCCATGAACAAAAATTATGGTTGCCGAAGGGAGAATTGCCATATGGCGAA‑GCGGCAAATTTCGATCTT                                                                         <  SRR4302545.39979/94‑1 (MQ=60)

                  TTAGATCACGGCTGGTGGGTCGTCAGCCATGAACAAAAATTATGGTTGCCGAAGGGAGAATTGCCATATGGCGAA‑GCGGCAAATTTCGATCTTG                                                                        >  SRR4302545.667226/1‑94 (MQ=60)

                  TTAGATCACGGCTGGTGGGTCGTCAGCCATGAACAAAAATTATGGTTGCCGAAGGGAGAATTGCCATATGGCGAA‑GCGGCAAATTTCGATCTTG                                                                        <  SRR4302545.3726933/94‑1 (MQ=60)

                   TAGATCACGGCTGGTGGGTCGTCAGCCATGAACAAAAATTATGGTTGCCGAAGGGAGAATTGCCATATGGCGAA‑GCGGCAAATTTCGATCTTGT                                                                       >  SRR4302545.2482714/1‑94 (MQ=60)

                    AGATCACGGCTGGTGGGTCGTCAGCCATGAACAAAAATTATGGTTGCCGAAGGGAGAATTGCCATATGGCGAA‑GCGGCAAATTTCGATCTTGTG                                                                      >  SRR4302545.1503673/1‑94 (MQ=60)

                    AGATCACGGCTGGTGGGTCGTCAGCCATGAACAAAAATTATGGTTGCCGAAGGGAGAATTGCCATATGGCGAA‑GCGGCAAATTTCGATCTTGTG                                                                      >  SRR4302545.716332/1‑94 (MQ=60)

                     GATCGCGGCTGGTGGGTCGTCAGCCATGAACAAAAATTATGGTTGCCGAAGGGAGAATTGCCATATGGCGAA‑GCGGCAAATTTCGATCTTGTGG                                                                     <  SRR4302545.8863445/94‑1 (MQ=60)

                      ATCACGGCTGGTGGGTCGTCAGCCATGAACAAAAATTATGGTTGCCGAAGGGAGAATTGCCATATGGCGAA‑GCGGCAAATTTCGATCTTGTGGG                                                                    <  SRR4302545.4105437/94‑1 (MQ=60)

                      ATCACGGCTGGTGGGTCGTCAGCCATGAACAAAAATTATGGTTGCCGAAGGGAGAATTGCCATATGGCGAA‑GCGGCAAATTTCGATCTTGTGGG                                                                    >  SRR4302545.4516279/1‑94 (MQ=60)

                      ATCACGGCTGGTGGGTCGTCAGCCATGAACAAAAATTATGGTTGCCGAAGGGAGAATTGCCATATGGCGAA‑GCGGCAAATTTCGATCTTGTGGG                                                                    >  SRR4302545.2492586/1‑94 (MQ=60)

                      ATCACGGCTGGTGGGTCGTCAGCCATGAACAAAAATTATGGTTGCCGAAGGGAGAATTGCCATATGGCGAA‑GCGGCAAATTTCGATCTTGTGGG                                                                    <  SRR4302545.1906032/94‑1 (MQ=60)

                       TCACGGCTGGTGGGTCGTCAGCCATGAACAAAAATTATGGTTGCCGAAGGGAGAATTGCCATATGGCGAA‑GCGGCAAATTTCGATCTTGTGGGT                                                                   >  SRR4302545.5795208/1‑94 (MQ=60)

                       TCACGGCTGGTGGGTCGTCAGCCATGAACAAAAATTATGGTTGCCGAAGGGAGAATTGCCATATGGCGAA‑GCGGCAAATTTCGATCTTGTGGGT                                                                   <  SRR4302545.2752002/94‑1 (MQ=60)

                        CACGGCTGGTGGGTCGTCAGCCATGAACAAAAATTATGGTTGCCGAAGGGAGAATTGCCATATGGCGAA‑GCGGCAAATTTCGATCTTGTGGGTC                                                                  <  SRR4302545.10063892/94‑1 (MQ=60)

                        CACGGCTGGTGGGTCGTCAGCCATGAACAAAAATTATGGTTGCCGAAGGGAGAATTGCCATATGGCGAA‑GCGGCAAATTTCGATCTTGTGGGTC                                                                  >  SRR4302545.5004684/1‑94 (MQ=60)

                        CACGGCTGGTGGGTCGTCAGCCATGAACAAAAATTATGGTTGCCGAAGGGAGAATTGCCATATGGCGAA‑GCGGCAAATTTCGATCTTGTGGGTC                                                                  >  SRR4302545.4197575/1‑94 (MQ=60)

                        CACGGCTGGTGGGTCGTCAGCCATGAACAAAAATTATGGTTGCCGAAGGGAGAATTGCCATATGGCGAA‑GCGGCAAATTTCGATCTTGTGGGTC                                                                  <  SRR4302545.10063891/94‑1 (MQ=60)

                         ACGGCTGGTGGGTCGTCAGCCATGAACAAAAATTATGGTTGCCGAAGGGAGAATTGCCATATGGCGAA‑GCGGCAAATTTCGATCTTGTGGGTCA                                                                 <  SRR4302545.9503516/94‑1 (MQ=60)

                          CGGCTGGTGGGTCGTCAGCCATGAAGAAAAATTATGGTTGCCGAAGGGAGAATTGCCATATGGCGAA‑GCGGCAAATTTCGATCTTGTGGGTCAG                                                                <  SRR4302545.2391325/94‑1 (MQ=60)

                          CGGCTGGTGGGTCGTCAGCCATGAACAAAAATTATGGTTGCCGAAGGGAGAATTGCCATATGGCGAA‑GCGGCAAATTTCGATCTTGTGGGTCAG                                                                <  SRR4302545.3741988/94‑1 (MQ=60)

                           GGCTGGTGGGTCGTCAGCCATGAACAAAAATTATGGTTGCCGAAGGGAGAATTGCCATATGGCGAA‑GCGGCAAATTTCGATCTTGTGGGTCAGC                                                               <  SRR4302545.6544334/94‑1 (MQ=60)

                           GGATGGTGGGTCGTCAGCCATGAACAAAAATTATGGTTGCCGAAGGGAGAATTGCCATATGGCGAA‑GCGGCAAATTTCGATCTTGTGGGTCAGC                                                               <  SRR4302545.6136605/94‑1 (MQ=60)

                            GCTGGTGGGTCGTCAGCCATGAACAAAAATTATGGTTGCCGAAGGGAGAATTGCCATATGGCGAA‑GCGGCAAATTTCGATCTTGTGGGTCAGCG                                                              <  SRR4302545.656818/94‑1 (MQ=60)

                            GCTGGTGGGTCGTCAGCCATGAACAAAAATTATGGTTGCCGAAGGGAGAATTGCCATATGGCGAA‑GCGGCAAATTTCGATCTTGTGGGTCAGCG                                                              <  SRR4302545.7013776/94‑1 (MQ=60)

                            GCTGGTGAGTCGTCAGCCATGAACAAAAATTATGGTTGCCGAAGGGAGAATTGCCATATGGCGAA‑GCGGCAAATTTCGATCTTGTGGGTCAGCG                                                              <  SRR4302545.9771157/94‑1 (MQ=60)

                             CTGGTGGGTCGTCAGCCATGAACAAAAATTATGGTTGCCGAAGGGAGAATTGCCATATGGCGAA‑GCGGCAAATTTCGATCTTGTGGGTCAGCGC                                                             >  SRR4302545.5182900/1‑94 (MQ=60)

                             CTGGTGGGTCGTCAGCCATGAACAAAAATTATGGTTGCCGAAGGGAGAATTGCCATATGGCGAA‑GCGGCAAATTTCGATCTTGTGGGTCAGCGC                                                             >  SRR4302545.6095229/1‑94 (MQ=60)

                             CTGGTGGGTCGTCAGCCATGAACAAAAATTATGGTTGCCGAAGGGAGAATTGCCATATGGCGAA‑GCGGCAAATTTCGATCATGTGGGTCAGCGC                                                             <  SRR4302545.6869287/94‑1 (MQ=60)

                              TGGTGGGTCGTCAGCCATGAACAAAAATTATGGTTGCCGAAGGGAGAATTGCCATATGGCGAA‑GCGGCAAATTTCGATCTTGTGGGTCAGCGCG                                                            >  SRR4302545.2247514/1‑94 (MQ=60)

                              TGGTGGGTCGTCAGCCATGAACAAAAATTATGGTTGCCGAAGGGAGAATTGCCATATGGCGAA‑GCGGCAAATTTCGATCTTGTGGGTCAGCGCG                                                            <  SRR4302545.1274412/94‑1 (MQ=60)

                                GTGGGTCGTCAGCCATGAACAAAAATTATGGTTGCCGAAGGGAGAATTGCCATATGGCGAA‑GCGGCAAATTTCGATCTTGTGGGTCAGCGCGCA                                                          >  SRR4302545.4664089/1‑94 (MQ=60)

                                 TGGGTCGTCAGCCATGAACAAAAATTATGGTTGCCGAAGGGAGAATTGCCATATGGCGAA‑GCGGCAAATTTCGATCTTGTGGGTCAGCGCGCAC                                                         >  SRR4302545.5090884/1‑94 (MQ=60)

                                  GGGTCGTCAGCCATGAACAAAAATTATGGTTGCCGAAGGGAGAATTGCCATATGGCGAA‑GCGGCAAATTTCGATCTTGTGGGTCAGCGCGCACT                                                        <  SRR4302545.8126853/94‑1 (MQ=60)

                                     TCGTCAGCCATGAACAAAAATTATGGTTGCCGAAGGGAGAATTGCCATATGGCGAA‑GCGGCAAATTTCGATCTTGTGGGTCAGCGCGCACTACA                                                     <  SRR4302545.8596382/94‑1 (MQ=60)

                                      CTTCAGCCATGAACAAAAAGTATGGTTGCCGAAGGGAGAATTGCCATATGGCGAA‑GCGGCAAATTTCGATCTTGTGGGTCAGCGCGCACTACAA                                                    <  SRR4302545.6157297/94‑1 (MQ=60)

                                      CGTCAGCCATGAACAAAAATTATGGTTGCCGAAGGGAGAATTGCCATATGGCGAA‑GCGGCAAATTTCGATCTTGTGGGTCAGCGCGCACTACAA                                                    <  SRR4302545.6157298/94‑1 (MQ=60)

                                       GTCAGCCATGAACAAAAATTATGGTTGCCGAAGGGAGAATTGCCATATGGCGAA‑GCGGCAAATTTCGATCTTGTGGGTCAGCGCGCACTACAAA                                                   <  SRR4302545.2183003/94‑1 (MQ=60)

                                        TCAGCCATGAACAAAAATTATGGTTGCCGAAGGGAGAATTGCCATATGGCGAA‑GCGGCAAATTTCGATCTTGTGGGTCAGCGCGCACTACAAAT                                                  >  SRR4302545.6424026/1‑94 (MQ=60)

                                         CAGCCATGAACAAAAATTATGGTTGCCGAAGGGAGAATTGCCATATGGCGAA‑GCGGCAAATTTCGATCTTGTGGGTCAGCGCGCACTACAAATC                                                 >  SRR4302545.5198347/1‑94 (MQ=60)

                                          AGCCATGAACAAAAATTATGGTTGCCGAAGGGAGAATTGCCATATGGCGAA‑GCGGCAAATTTCGATCTTGTGGGTCAGCGCGCACTACAAATCG                                                <  SRR4302545.4320491/94‑1 (MQ=60)

                                          AGCCATGAACAAAAATTATGGTTGCCGAAGGGAGAATTGCCATATGGCGAA‑GCGGCAAATTTCGATCTTGTGGGTCAGCGCGCACTACAAATCG                                                <  SRR4302545.5378180/94‑1 (MQ=60)

                                          AGCCATGAACAAAAATTATGGTTGCCGAAGGGAGAATTGCCATATGGCGAA‑GCGGCAAATTTCGATCTTGTGGGTCAGCGCGCACTACAAATCG                                                >  SRR4302545.2012628/1‑94 (MQ=60)

                                          AGCCATGAACAAAAATTATGGTTGCCGAAGGGAGAATTGCCATATGGCGAA‑GCGGCAAATTTCGATCTTGTGGGTCAGCGCGCACTACAAATCG                                                <  SRR4302545.4166263/94‑1 (MQ=60)

                                           GCCATGAACAAAAATTATGGTTGCCGAAGGGAGAATTGCCATATGGCGAA‑GCGGCAAATTTCGATCTTGTGGGTCAGCGCGCACTACAAATCGG                                               >  SRR4302545.1222613/1‑94 (MQ=60)

                                           GCCATGAACAAAAATTATGGTTGCCGAAGGGAGAATTGCCATATGGCGAA‑GCGGCAAATTTCGATCTTGTGGGTCAGCGCGCACTACAAATCGG                                               <  SRR4302545.10461553/94‑1 (MQ=60)

                                           GCCATGAACAAAAATTATGGTTGCCGAAGGGAGAATTGCCATATGGCGAA‑GCGGCAAATTTCGATCTTGTGGGTCAGCGCGCACTACAAATCGG                                               <  SRR4302545.8591377/94‑1 (MQ=60)

                                           GCCATGAACAAAAATTATGGTTGCCGAAGGGAGAATAGCCATATGGCGAA‑GCGGCAAATTTCGATCTTGTGGGTCAGCGCGCACTACAAATCGG                                               <  SRR4302545.3367788/94‑1 (MQ=60)

                                            CCATGAACAAAAATTATGGTTGCCGAAGGGAGAATTGCCATATGGCGAA‑GCGGCAAATTTCGATCTTGTGGGTCAGCGCGCACTACAAATCGGC                                              <  SRR4302545.182074/94‑1 (MQ=60)

                                            CCATGAACAAAAATTATGGTTGCCGAAGGGAGAATTGCCATATGGCGAA‑GCGGCAAATTTCGATCTTGTGGGTCAGCGCGCACTACAAATCGGC                                              <  SRR4302545.3540562/94‑1 (MQ=60)

                                             CATGAACAAAAATTATGGTTGCCGAAGGGAGAATTGCCATATGGCGAA‑GCGGCAAATTTCGATCTTGTGGGTCAGCGCGCACTACcactccgcg                                             >  SRR4302545.162687/1‑85 (MQ=60)

                                             CATGAACAAAAATTATGGTTGCCGAAGGGAGAATTGCCATATGGCGAA‑GCGGCAAATTTCGATCTTGTGGGTCAGCGCGCACTACAAATCGGCA                                             >  SRR4302545.1118829/1‑94 (MQ=60)

                                              ATGAGCAAAAATTATGGTTGCCGAAGGGAGAATTGCCATATGGCGAA‑GCGGCAAATTTCGATCTTGTGGGTCAGCGCGCACTACAAATCGGCGA                                            >  SRR4302545.1246216/1‑94 (MQ=60)

                                              ATGAACAAAAATTATGGTTGCCGAAGGGAGAATTGCCATATGGCGAA‑GCGGCAAATTTCGATCTTGTGGGTCAGCGCGCACTACAAATCGGCGA                                            >  SRR4302545.1246215/1‑94 (MQ=60)

                                              ATGAACAAAAATTATGGTTGCCGAAGGGAGAATTGCCATATGGCGAA‑GCGGCAAATTTCGATCTTGTGGGTCAGCGCGCACTACAAATCGGCGA                                            >  SRR4302545.6627236/1‑94 (MQ=60)

                                                GAACAAAAATTATGGTTGCCGAAGGGAGAATTGCCATATGGCGAA‑GCGGCAAATTTCGATCTTGTGGGTCAGCGCGCACTACAAATCGGCGAAT                                          <  SRR4302545.6864389/94‑1 (MQ=60)

                                                GAACAAAAATTATGGTTGCCGAAGGGAGAATTGCCATATGGCGAA‑GCGGCAAATTTCGATCTTGTGGGTCAGCGCGCACGACAAATCGGCGAAT                                          <  SRR4302545.5519201/94‑1 (MQ=60)

                                                 AACAAAAATTATGGTTGCCGAAGGGAGAATTGCCATATGGCGAA‑GCGGCAAATTTCGATCTTGTGGGTCAGCGCGCACTACAAGTCGGCGAATG                                         <  SRR4302545.646308/94‑1 (MQ=60)

                                                 AACAAAAATTATGGTTGCCGAAGGGAGAATTGCCATATGGCGAA‑GCGGCAAATTTCGATCTTGTGGGTCAGCGCGCACTACAAATCGGCGAATG                                         <  SRR4302545.3663131/94‑1 (MQ=60)

                                                 AACAAAAATTATGGTTGCCGAAGGGAGAATTGCCATATGGCGAA‑GCGGCAAATTTCGATCTTGTGGGTCAGCGCGCACTACAAATCGGCGAATG                                         <  SRR4302545.9219653/94‑1 (MQ=60)

                                                 AACAAAAATTATGGTTGCCGAAGGGAGAATTGCCATATGGCGAA‑GCGGCAAATTTCGATCTTGTGGGTCAGCGCGCACTACAAATCGGCGAATG                                         >  SRR4302545.290478/1‑94 (MQ=60)

                                                 AACAAAAATTATGGTTGCCGAAGGGAGAATTGCCATATGGCGAA‑GCGGCAAATTTCGATCTTGTGGGTCAGCGCGCACTACAAATCGGCGAATG                                         <  SRR4302545.3663133/94‑1 (MQ=60)

                                                  ACAAAAATTATGGTTGCCGAAGGGAGAATTGCCATATGGCGAA‑GCGGCAAATTTCGATCTTGTGGGTCAGCGCGCACTACAAATCGGCGAATGG                                        <  SRR4302545.6806991/94‑1 (MQ=60)

                                                  ACAAAAATTATGGTTGCCGAAGGGAGAATTGCCATATGGCGAA‑GCGGCAAATTTCGATCTTGTGGGTCAGCGCGCACTACAAATCGGCGAATGG                                        <  SRR4302545.729509/94‑1 (MQ=60)

                                                   CAAAAATTATGGTTGCCGAAGGGAGAATTGCCATATGGCGAA‑GCGGCAAATTTCGATCTTGTGGGTCAGCGCGCACTACAAATCGGCGAATGGC                                       >  SRR4302545.6465867/1‑94 (MQ=60)

                                                   CAAAAATTATGGTTGCCGAAGGGAGAATTGCCATATGGCGAA‑GCGGCAAATTTCGATCTTGTGGGTCAGCGCGCACTACAAATCGGCGAATGGC                                       <  SRR4302545.8523278/94‑1 (MQ=60)

                                                   CAAAAAGTATGGTTGCCGAAGGGAGAATTGCCATATGGCGAA‑GCGGCAAATTTCGATCTTGTGGGTCAGCGCGCACTACAAATCGGCGAATGGC                                       <  SRR4302545.10430837/94‑1 (MQ=60)

                                                    AAAAATTCTGGTTGCCGGAGGGAGTTTCTCCATATGGCGAA‑TCGGCAAATTTCGATCTTGTGTTTCAGAGCGCACTACAAATCGGCGtgtgtgg                                      >  SRR4302545.10196000/1‑87 (MQ=22)

                                                    AAAAATTATGGTTGCCGAAGGGAGAATTGCCATATGGCGAA‑GCGGCAAATTTCGATCTTGTGGGTCAGCGCGCACTACAAATCGGCGAATGGCA                                      >  SRR4302545.250845/1‑94 (MQ=60)

                                                    AAAAATTATGGTTGCCGAAGGGAGAATTGCCATATGGCGAA‑GCGGCAAATTTCGATCTTGTGGGTCAGCGCGCACTACAAATCGGCGAATGGCA                                      <  SRR4302545.8443427/94‑1 (MQ=60)

                                                    AAAAATTATGGTTGCCGAAGGGAGAATTGCCATATGGCGAA‑GCGGCAAATTTCGATCTTGTGGGTCAGCGCGCACTACAAATCGGCGAATGGCA                                      >  SRR4302545.3584256/1‑94 (MQ=60)

                                                     AAAATTATGGTTGCCGAAGGGAGAATTGCCATATGGCGAA‑GCGGCAAATTTCGATCTTGTGGGTCAGCTCGCACTACAAATCGGCGAATGGCAG                                     >  SRR4302545.5817345/1‑94 (MQ=60)

                                                     AAAATTATGGTTGCCGAAGGGAGAATTGCCATATGGCGAA‑GCGGCAAATTTCGATCTTGTGGGTCAGCGCGCACTACAAATCGGCGAATGGCAG                                     <  SRR4302545.413402/94‑1 (MQ=60)

                                                     AAAATTATGGTTGCCGAAGGGAGAATTGCCATATGGCGAA‑GCGGCAAATTTCGATCTTGTGGGTCAGCGCGCACTACAAATCGGCGAATGGCAG                                     <  SRR4302545.627254/94‑1 (MQ=60)

                                                     AAAATTATGGTTGACGAAAGGAGAATTGGCGTAGGGCGAA‑GCGGCAAATTTCGATCTTGTGGGTCATCGCGCACTACAAATCGGCGAATGGCAG                                     <  SRR4302545.5201839/94‑1 (MQ=60)

                                                      AAATTATGGTTGCCGAAGGGAGAATTGCCATATGGCGAA‑GCGGCAAATTTCGATCTTGTGGGTCAGCGCGCACTACAAATCGGCGAATGGCAGG                                    <  SRR4302545.7660946/94‑1 (MQ=60)

                                                      AAATTATGGTTGCCGAAGGGAGAATTGCCATATGGCGAA‑GCGGCAAATTTCGATCTTGTGGGTCAGCGCGCACTACAAATCGGCGAATGGCAGG                                    <  SRR4302545.228969/94‑1 (MQ=60)

                                                      AAATTATGGTTGCCGAAGGGAGAATTGCCATATGGCGAA‑GCGGCAAATTTCGATCTTGTGGGTCAGCGCGCACTACAAATCGGCGAATGGCAGG                                    <  SRR4302545.3851416/94‑1 (MQ=60)

                                                       AATTATGGTTGCCGAAGGGAGAATTGCCATATGGCGAA‑GCGGCAAATTTCGATCTTGTGGGTCAGCGCGCACTACAAATCGGCGAATGGCAGGG                                   <  SRR4302545.2369250/94‑1 (MQ=60)

                                                       AATTATGGTTGCCGAAGGGAGAATTGCCATATGGCGAA‑GCGGCAAATTTCGATCTTGTGGGTCAGCGCGCACTACAAATCGGCGAATGGCAGGG                                   <  SRR4302545.4730195/94‑1 (MQ=60)

                                                       AATTATGGTTGCCGAAGGGAGAATTGCCATATGGCGAA‑GCGGCAAATTTCGATCTTGTGGGTCAGCGCGCACTACAAATCGGCGAATGGCAGGG                                   <  SRR4302545.9420201/94‑1 (MQ=60)

                                                       AATTATGGTTGCCGAAGGGAGAATTGCCATATGGCGAA‑GCGGCAAATTTCGATCTTGTGGGTCAGCGCGCACTACAAATCGGCGAATGGCAGGG                                   <  SRR4302545.4513591/94‑1 (MQ=60)

                                                       AATTATGGTTGCCGAAGGGAGAATTGCCATATGGCGAA‑GCAGCAAATTTCGATCTTGTGGGTCAGCGCGCACTACAAATCGGCGAATGGCAGGG                                   <  SRR4302545.5454483/94‑1 (MQ=60)

                                                       AATTATGGCTGCCGAAGGGAGAATTGCCATATGGCGAA‑GCGGCAAATTTCGATCTTGTGGGTCAGCGCGCACTACAAATCGGCGAATGGCAGGG                                   <  SRR4302545.5332336/94‑1 (MQ=60)

                                                        ATTATGGTTGCCGAAGGGAGAATTGCCATATGGCGAA‑GCGGCAAATTTCGATCTTGTGGGTCAGCGCGCACTACAAATCGGCGAATGGCAGGGG                                  >  SRR4302545.7177840/1‑94 (MQ=60)

                                                        ATTATGGTTGCCGAAGGGAGAATTGCCATATGGCGAA‑GCGGCAAATTTCGATCTTGTGGGTCAGCGCGCACTACAAATCGGCGAATGGCAGGGG                                  <  SRR4302545.3475394/94‑1 (MQ=60)

                                                        ATTATGGTTGCCGAAGGGAGAATTGCCATATGGCGAA‑GCGGCAAATTTCGATCTTGTGGGTCAGCGCGCACTACAAATCGGCGAATGGCAGGGG                                  <  SRR4302545.10234939/94‑1 (MQ=60)

                                                         TTATGGTTGCCGAAGGGAGAATTGCCATATGGCGAT‑GCGGCAAATTTCGATCTTGTGGGTCAGCGCGCACTACAAATCGGCGAATGGCAGGGGG                                 >  SRR4302545.7067053/1‑94 (MQ=60)

                                                         TTATGGTTGCCGAAGGGAGAATTGCCATATGGCGAA‑GCGGCAAATTTCGATCTTGTGGGTCAGCGCGCACTACAAATCGGCGAATGGCAGGGGG                                 >  SRR4302545.3452162/1‑94 (MQ=60)

                                                         TTATGGTTGCCGAAGGGAGAATTGCCATATGGCGAA‑GCGGCAAATTTCGATCTTGTGGGTCAGCGCGCACTACAAATCGGCGAATGGCAGGGGG                                 <  SRR4302545.9599802/94‑1 (MQ=60)

                                                         TTATGGTTGCCGAAGGGAGAATTGCCATATGGCGAA‑GCGGCAAATTTCGATCTTGTGGGTCAGCGCGCACTACAAATCGGCGAATGGCAGGGGG                                 <  SRR4302545.7317711/94‑1 (MQ=60)

                                                         ATATGGTTGCCGAAGGGAGAATTGCCATATGGCGAA‑GCGGCAAATTTCGATCTTGTGGGTCAGCGCGCACTACAAATCGGCGAATGGCAGGGGG                                 <  SRR4302545.3697995/94‑1 (MQ=60)

                                                          TATGGTTGCCGAAGGGAGAATTGCCATATGGCGAA‑GCGGCAAATTTCGATCTTGTGGGTCAGCGCGCACTACAAATCGGCGAATGGCAGGGGGA                                <  SRR4302545.5458339/94‑1 (MQ=60)

                                                          TATGGTTGCCGAAGGGAGAATTGCCATATGGCGAA‑GCGGCAAATTTCGATCTTGTGGGTCAGCGCGCACTACAAATCGGCGAATGGCAGGGGGA                                <  SRR4302545.3329010/94‑1 (MQ=60)

                                                          TATGGTTGCCGAAGGGAGAATTGCCATATGGCGAA‑GCGGCAAATTTCGATCTTGTGGGTCAGCGCGCACTACAAATCGGCGAATGGCAGGGGGA                                <  SRR4302545.8978813/94‑1 (MQ=60)

                                                           ATGGTTGCCGAAGGGAGAATTGCCATATGGCGAA‑GCGGCAAATTTCGATCTTGTGGGTCAGCGCGCACTACAAATCGGCGAATGGCAGGGGGAA                               <  SRR4302545.2041617/94‑1 (MQ=60)

                                                           ATGGTTGCCGAAGGGAGAATTGCCATATGGCGAA‑GCGGCAAATTTCGATCTTGTGGGTCAGCGCGCACTACAAATCGGCGAATGGCAGGGGGAA                               <  SRR4302545.2171505/94‑1 (MQ=60)

                                                           ATGGTTGCCGAAGGGAGAATTGCCATATGGCGAA‑GCGGCAAATTTCGATCTTGTGGGTCAGCGCGCACTACAAATCGGCGAATGGCAGGGGGAA                               <  SRR4302545.8295177/94‑1 (MQ=60)

                                                             GGTTGCCGAAGGGAGAATTGCCATATGGCGAA‑GCGGCAAATTTCGATCTTGTGGGTCAGCGCGCACTACAAATCGGCGAATGGCAGGGGGAACC                             <  SRR4302545.3858693/94‑1 (MQ=60)

                                                              GTTGCCGAAGGGAGAATTGCCATATGGCGAA‑GCGGCAAATTTCGATCTTGTGGGTCAGCGCGCACTACAAATCGGCGAATGGCAGGGGGAACCT                            <  SRR4302545.2048342/94‑1 (MQ=60)

                                                              GTTGCCGAAGGGAGAATTGCCATATGGCGAA‑GCGGCAAATTTCGATCTTGTGGGTCAGCGCGCACTACAAATCGGCGAATGGCAGGGGGAACCT                            <  SRR4302545.7030084/94‑1 (MQ=60)

                                                              GTTGCCGAAGGGAGAATTGCCATATGGCGAA‑GCGGCAAATTTCGATCTTGTGGGTCAGCGCGCACTACAAATCGGCGAATGGCAGGGGGAACCT                            <  SRR4302545.8929822/94‑1 (MQ=60)

                                                               TTGCCGAAGGGAGAATTGCCATATGGCGAA‑GCGGCAAATTTCGATCTTGTGGGTCAGCGCGCACTACAAATCGGCGAATGGCAGGGGGAACCTG                           <  SRR4302545.1127742/94‑1 (MQ=60)

                                                                TGCCGAAGGGAGAATTGCCATATGGCGAA‑GCGGCAAATTTCGATCTTGTGGGTCAGCGCGCACTACAAATCGGCGAATGGCAGGGGGAACCTGT                          <  SRR4302545.5227766/94‑1 (MQ=60)

                                                                TGCCGAAGGGAGAATTGCCATATGGCGAA‑GCGGCAAATTTCGATCTTGTGGGTCAGCGCGCACTACAAATCGGCGAATGGCAGGGGGAACCTGT                          <  SRR4302545.4660983/94‑1 (MQ=60)

                                                                TGCCGAAGGGAGAATTGCCATATGGCGAA‑GCGGCAAATTTCGATCTTGTGGGTCAGCGCGCACTACAAATCGGCGAATGGCAGGGGGAACCTGT                          <  SRR4302545.5227764/94‑1 (MQ=60)

                                                                 GCCGAAGGGAGAATTGCCATATGGCGAA‑GCGGCAAATTTCGATCTTGTGGGTCAGCGCGCACTACAAATCGGCGAATGGCAGGGGGAACCTGTT                         <  SRR4302545.11461/94‑1 (MQ=60)

                                                                  CCGAAGGGAGAATTGCCATATGGCGAA‑GCGGCAAATTTCGATCTTGTGGGTCAGCGCGCACTACAAATCGGCGAATGGCAGGGGGAACCTGTTT                        <  SRR4302545.1102381/94‑1 (MQ=60)

                                                                  CCGAAGGGAGAATTGCCATATGGCGAA‑GCGGCAAATTTCGATCTTGTGGGTCAGCGCGCACTACAAATCGGCGAATGGCAGGGGGAACCTGTTT                        <  SRR4302545.2157991/94‑1 (MQ=60)

                                                                  CCGAAGGGAGAATTGCCATATGGCGAA‑GCGGCAAATTTCGATCTTGTGGGTCAGCGCGCACTACAAATCGGCGAATGGCAGGGGGAACCTGTTT                        <  SRR4302545.4793343/94‑1 (MQ=60)

                                                                  CCGAAGGGAGAATTGCCATATGGCGAA‑GCGGCAAATTTCGATCTTGTGGGTCAGCGCGCACTACAAATCGGCGAATGGCAGGGGGAACCTGTTT                        <  SRR4302545.191766/94‑1 (MQ=60)

                                                                  CCGAAGGGAGAATTGCCATATGGCGAA‑GCGGCAAATTTCGATCTTGTGGGTCAGCGCGCACTACAAATCGGCGAATGGCAGGGGGAACCTGTTT                        <  SRR4302545.3293244/94‑1 (MQ=60)

                                                                  CCGAAGGGAGAATTGCCATATGGCGAA‑GCGGCAAATTTCGATCTTGTGGGTCAGCGCGCACTACAAATCGGCGAATGGCAGGGGGAACCTGTTT                        <  SRR4302545.7298638/94‑1 (MQ=60)

                                                                  CCGAAGGGAGAATTGCCATATGGCGAA‑GCGCCAAATTTCGATCTTGTGGGTCAGCGCGCACTACAAATCGGCGAATGGCAGGGGGAACCTGTTT                        <  SRR4302545.4585624/94‑1 (MQ=60)

                                                                   CGAAGGGAGAATTGCCATATGGCGAA‑GCGGCAAATTTCGATCTTGTGGGTCAGCGCGCACTACAAATCGGCGAATGGCAGGGGGAACCTGTTTG                       >  SRR4302545.8850489/1‑94 (MQ=60)

                                                                    GAAGGGAGAATTGCCATATGGCGCA‑GCGGCAAATTTCGATCTTGTGGGTCAGCGCGCACTACAAATCGGCGAATGGCAGGGGGAACCTGTTTGG                      <  SRR4302545.7447409/94‑1 (MQ=60)

                                                                    GAAGGGAGAATTGCCATATGGCGAA‑GCGGCAAATTTCGATCTTGTGGGTCAGCGCGCACTACAAATCGGCGAATGGCAGGGGGAACCTGTTTGG                      >  SRR4302545.5834848/1‑94 (MQ=60)

                                                                    GAAGGGAGAATTGCCATATGGCGAA‑GCGGCAAATTTCGATCTTGTGGGTCAGCGCGCACTACAAATCGGCGAATGGCAGGGGGAACCTGTTTGG                      <  SRR4302545.7447408/94‑1 (MQ=60)

                                                                    GAAGGGAGAATTGCCATATGGCGAA‑GCGGCAAATTTCGATCTTGTGGGTCAGCGCGCACTACAAATCGGCGAATGGCAGGGGGAACCTGTTTGG                      <  SRR4302545.1270694/94‑1 (MQ=60)

                                                                    GAAGGGAGAATTGCCATATGGCGAA‑GCGGCAAATTTCGATCTTGTGGGTCAGCGCGCACTACAAATCGGCGAATGGCAGGGGGAACCTGTTTGG                      <  SRR4302545.6237197/94‑1 (MQ=60)

                                                                     AAGGGAGAATTGCCATATGGCGAA‑GCGGCAAATTTCGATCTTGTGGGTGAGCGCGCACTACAAATTCGAGAATGGCAGGGGGAAgctggttcgt                     >  SRR4302545.6348144/1‑84 (MQ=60)

                                                                      AGGGAGAATTGCCATATGGCGAA‑GCGGCAAATTTCGATCTTGTGGGTCAGCGCGCACTACAAATCGGCGAATGGCAGGGGGAACCTGTTTGGTT                    <  SRR4302545.2101086/94‑1 (MQ=60)

                                                                       GGGAGAATTGCCATATGGCGAA‑GCGGCAAATTTCGATCTTGTGGGTCAGCGCGCACTACAAATCGGCGAATGGCAGGGGGAACCTGTTTGGTTA                   <  SRR4302545.1090065/94‑1 (MQ=60)

                                                                       GGGAGAATTGCCATATGGCGAA‑GCGGCAAATTTCGATCTTGTGGGTCAGCGCGCACTACAAATCGGCGAATGGCAGGGGGAACCTGTTTGGGTA                   >  SRR4302545.863656/1‑94 (MQ=60)

                                                                        GGAGAATTGCCATATGGCGAA‑GCGGCAAATTTCGATCTTGTGGGTCAGCGCGCACTACAAATCGGCGAATGGCAGGGGGAACCTGTTTGGTTAG                  <  SRR4302545.607837/94‑1 (MQ=60)

                                                                        GGAGAATTGCCATATGGCGAA‑GCGGCAAATTTCGATCTTGTGGGTCAGCGCGCACTACAAATCGGCGAATGGCAGGGGGAACCTGTTTGGTTAG                  <  SRR4302545.1254390/94‑1 (MQ=60)

                                                                        GGAGAATTGCCATATGGCGAA‑GCGGCAAATCTCGATCTTGTGGGTCAGCGCGCACTACAAATCGGCGAATGGCAGGGGGAACCTGTTTGGTTAG                  <  SRR4302545.6469254/94‑1 (MQ=60)

                                                                         GAGAATTGCCATATGGCGAA‑GCGGCAAATTTCGATCTTGTGGGTCAGCGCGCACTACAAATCGGCGAATGGCAGGGGGAACCTGTTTGGTTAGT                 <  SRR4302545.1733680/94‑1 (MQ=60)

                                                                          AGAATTGCCATATGGCGAA‑GCGGCAAATTTCGATCTTGTGGGTCAGCGCGCACTACAAATCGGCGAATGGCAGGGGGAACCTGTTTGGTTAGTA                >  SRR4302545.4882959/1‑94 (MQ=60)

                                                                          AGAATTGCCATATGGCGAA‑GCGGCAAATTTCGATCTTGTGGGTCAGCGCGCACTACAAATCGGCGAATGGCAGGGGGAACCTGTTTGGTTAGTA                <  SRR4302545.6240844/94‑1 (MQ=60)

                                                                          AGAATTGCCATATGGCGAA‑GCGGCAAATTTCGATCTTGTGGGTCAGCGCGCACTACAAATCGGCGAATGGCAGGGGGAACCTGTTTGGTTAGTA                >  SRR4302545.8591861/1‑94 (MQ=60)

                                                                           GAATTGCCATATGGCGAA‑GCGGCAAATTTCGATCTTGTGGGTCAGCGCGCACTACAAATCGGCGAATGGCAGGGGGAACCTGTTTGGTTAGTAC               <  SRR4302545.7352265/94‑1 (MQ=60)

                                                                           GAATTGCCATATGGCGAA‑GCGGCAAATTTCGATCTTGTGGGTCAGCGCGCACTACAAATCGGCGAATGGCAGGGGGAACCTGTTTGGTTAGTAC               >  SRR4302545.7824473/1‑94 (MQ=60)

                                                                            AATTGCCATATGGCGAA‑GCGGCAAATTTCGATCTTGTGGGTCAGCGCGCACTACAAATCGGCGAATGGCAGGGGGAACCTGTTTGGTTAGTACA              <  SRR4302545.1210882/94‑1 (MQ=60)

                                                                            AATTGCCATATGGCGAA‑GCGGCAAATTTCGATCTTGTGGGTCAGCGCGCACTACAAATCGGCGAATGGCAGGGGGAACCTGTTTGGTTAGTACA              <  SRR4302545.7254563/94‑1 (MQ=60)

                                                                             ATTGCCATATGGCGAA‑GCGGCAAATTTCGATCTTGTGGGTCAGCGCGCACTACAAATCGGCGAATGGCAGGGGGAACCTGTTTGGTTAGTACAA             >  SRR4302545.8142664/1‑94 (MQ=60)

                                                                              TTGCCATATGGCGAA‑GCGGCAAATTTCGATCTTGTGGGTCAGCGCGCACTACAAATCGGCGAATGGCAGGGGGAACCTGTTTGGTTATTACAAC            <  SRR4302545.3580409/94‑1 (MQ=60)

                                                                              TTGCCATATGGCGAA‑GCGGCAAATTTCGATCTTGTGGGTCAGCGCGCACTACAAATCGGCGAATGGCAGGGGGAACCTGTTTGGTTAGTACAAC            <  SRR4302545.3617090/94‑1 (MQ=60)

                                                                              TTGCCATATGGCGAA‑GCGGCAAATTTCGATCTTGTGGGTCAGCGCGCACTACAAATCGGCGAATGGCAGGGGGAACCTGTTTGGTTAGTACAAC            <  SRR4302545.1071028/94‑1 (MQ=60)

                                                                                TCCATATGGCGAA‑GCGGCAAATTTCGATCTTGTGGGTCAGCGCGCACTACAAATCGGCGAATGGCAGGGGGAACCTGTTTGGTTAGTACAACAG          <  SRR4302545.1470074/94‑1 (MQ=60)

                                                                                GCCATATGGCGAA‑GCGGCAAATTTCGATCTTGTGGGTCAGCGCGCACTACAAATCGGCGAATGGCAGGGGGAACCTGTTTGGTTAGTACAACAG          >  SRR4302545.7006033/1‑94 (MQ=60)

                                                                                GCCATATGGCGAA‑GCGGCAAATTTCGATCTTGTGGGTCAGCGCGCACTACAAATCGGCGAATGGCAGGGGGAACCTGTTTGGTTACTACAACAG          >  SRR4302545.3926292/1‑94 (MQ=60)

                                                                                 CCATATGGCGAA‑GCGGCAAATTTCGATCTTGTGGGTCAGCGCGCACTACAAATCGGCGAATGGCAGGGGGAACCTGTTTGGTTAGTACAACAGC         <  SRR4302545.7302968/94‑1 (MQ=60)

                                                                                 CCATATGGCGAA‑GCGGCAAATTTCGATCTTGTGGGTCAGCGCGCACTACAAATCGGCGAATGGCAGGGGGAACCTGTTTGGTTAGTACAACAGC         <  SRR4302545.5570583/94‑1 (MQ=60)

                                                                                  CATATGGCGAA‑GCGGCAAATTTCGATCTTGTGGGTCAGCGCGCACTACAAATCGGCGAATGGCAGGGGGAACCTGTTTGGTTAGTACAACAGCA        <  SRR4302545.9824455/94‑1 (MQ=60)

                                                                                  CATATGGCGAA‑GCGGCAAATTTCGATCTTGTGGGTCAGCGCGCACTACAAATCGGCGAATGGCAGGGGGAACCTGTTTGGTTAGTACAACAGCA        >  SRR4302545.3968506/1‑94 (MQ=60)

                                                                                  CATATGGCGAA‑GCGGCAAATTTCGATCTTGTGGGTCAGCGCGCACTACAAATCGGCGAATGGCAGGGGGAACCTGTTTGGTTAGTACAACAGCA        <  SRR4302545.8736119/94‑1 (MQ=60)

                                                                                   ATATGGCGAA‑GCGGCAAATTTCGATCTTGTGGGTCAGCGCGCACTACAAATCGGCGAATGGCAGGGGGAACCTGTTTGGTTAGTACAACAGCAG       >  SRR4302545.3315061/1‑94 (MQ=60)

                                                                                   ATATGGCGAA‑GCGGCAAATTTCGATCTTGTGGGTCAGCGCGCACTACAAATCGGCGAATGGCAGGGGGAACCTGTTTGGTTAGTACAACAGCAG       >  SRR4302545.7849234/1‑94 (MQ=60)

                                                                                    TATGGCGAA‑GCGGCAAATTTCGATCTTGTGGGTCAGCGCGCACTACAAATCGGCGAATGGCAGGGGGAACCTGTTTGGTTAGTACAACAGCAGC      <  SRR4302545.6055468/94‑1 (MQ=60)

                                                                                    TATGGCGAA‑GCGGCAAATTTCGATCTTGTGGGTCAGCGCGCACTACAAATCGGCGAATGGCAGGGGGAACCTGTTTGGTTAGTACAACAGCAGC      <  SRR4302545.320851/94‑1 (MQ=60)

                                                                                     ATGGCGAA‑GCGGCAAATTTCGATCTTGTGGGTCAGCGCGCACTACAAATCGGCGAATGGCAGGGGGAACCTGTTTGGTTAGTACAACAGCAGCG     <  SRR4302545.9915217/94‑1 (MQ=60)

                                                                                     ATGGCGAA‑GCGGCAAATTTCGATCTTGTGGGTCAGCGCGCACTACAAATCGGCGAATGGCAGGGGGAACCTGTTTGGTTAGTACAACAGCAGCG     <  SRR4302545.7315011/94‑1 (MQ=60)

                                                                                      TGGCGAA‑GCGGCAAATTTCGATCTTGTGGGTCAGCGCGCACTACAAATCGGCGAATGGCAGGGGGAACCTGTTTGGTTAGTACAACAGCAGCGG    <  SRR4302545.8019595/94‑1 (MQ=60)

                                                                                      TGGCGAA‑ACGGCAAATTTCGATCTTGTGGGTCAGCGCGCACTACAAATCGGCGAATGGCAGGGGGAACCTGTTTGGGTAGTAacacagaagcag    >  SRR4302545.7776141/1‑82 (MQ=60)

                                                                                       GGCGAA‑GCGGCAAATTTCGATCTTGTTGGTCAGCGCGCACTACAAATCGGCGAATGGCAGGGGGAACCTGTTTGGTTAGTACAACAGCAGCGGC   <  SRR4302545.3462094/94‑1 (MQ=60)

                                                                                       GGCGAA‑GCGGCAAATTTCGATCTTGTGGGTCAGCGCGCACTACAAATCGGCGAATGGCAGGGGGAACCTGTTTGGTTAGTACAACAGCAGCGGC   <  SRR4302545.3462093/94‑1 (MQ=60)

                                                                                       GGCGAA‑GCGGCAAATTTCGATCTTGTGGGTCAGCGCGCACTACAAATCGGCGAATGGCAGGGGGAACCTGTTTGGTTAGTACAACAGCAGCGGC   >  SRR4302545.7344405/1‑94 (MQ=60)

                                                                                        GCGAA‑GCGGCAAATTTCGATCTTGTGGGTCAGCGCGCACTACAAATCGGCGAATGGCAGGGGGAACCTGTTTGGTTAGTACAACAGCAGCGGCG  <  SRR4302545.6166889/94‑1 (MQ=60)

                                                                                        GCGAA‑GCGGCAAATTTCGATCTTGTGGGTCAGCGCGCACTACAAATCGGCGAATGGCAGGGGGAACCTGTTTGGTTAGTACAACAGCAGCGGCG  <  SRR4302545.6166890/94‑1 (MQ=60)

                                                                                        GCGAA‑GCGGCAAATTTCGATCTTGTGGGTCAGCGCGCACTACAAATCGGCGAATGGCAGGGGGAACCTGTTTGGTTAGTACAACAGCAGCGGCG  <  SRR4302545.1484892/94‑1 (MQ=60)

                                                                                        GCGAA‑GCGGCAAATTTCGATCTTGTGGGTCAGCGCGCACTACAAATCGGCGAATGGCAGGGGGAACCTGTTTGGTTAGTACAACAGCAGCGGCG  <  SRR4302545.1484891/94‑1 (MQ=60)

GATCGTATAATTGAAAAATTAGATCACGGCTGGTGGGTCGTCAGCCATGAACAAAAATTATGGTTGCCGAAGGGAGAATTGCCATATGGCGAA‑CGGGCAAATTTCGATCTTGTGGGTCAGCGCGCACTACAAATCGGCGAATGGCAGGGGGAACCTGTTTGGTTAGTACAACAGCAGCGGCG  >  NC_000913/4194484‑4194665

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 0 ≤` `ATCG/ATCG` `< 2 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`