Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I1 R1 647 270.1 21258240 98.4% 20918108 94.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 4,302,330 A→G V620A (GTG→GCG)  yjcS ← hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,302,3300AG99.4% 1056.2 / ‑3.6 310V620A (GTG→GCG) yjcShypothetical protein
Reads supporting (aligned to +/- strand):  ref base A (1/1);  new base G (242/66);  total (243/67)
Fisher's exact test for biased strand distribution p-value = 3.86e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.10e-01

Only 100 of 337 total aligned reads displayed.

GGCGATAATTTCTTCCAGTTTCGCGCCATTGCCAATAATTTTGGCTTTCTTCGCTTTTACCAGATCCGCCATTTTGGCTTGTCCGGTCAGCACGGCGTGCAGATCTTCACGGCTGATGTAGAAAGAGGCGTCGGCTTGC‑GGTTGCAGCGTTTTCCGGTAGTTAAGCACGCTATCGTTCAGCG  >  NC_000913/4302239‑4302420
                                                                                           |                                                                                           
ggCGATAATTTCTTCCAGTTTCGCGCCATTGCCAATAATTTTGGCTTTCTTCGCTTTTACCAGATCCGCCATTTTGGCTTGTCCGGTCAGcgcg                                                                                           >  2:7053679/1‑94 (MQ=255)
 gCGATAATTTCTTCCAGTTTCGCGCCATTGCCAATAATTTTGGCTTTCTTCGCTTTTACCAGATCCGCCATTTTGGCTTGTCCGGTCAGCGCgg                                                                                          >  2:6160160/1‑94 (MQ=255)
  cGATAATTTCTTCCAGTTTCGCGCCATTGCCAATAATTTTGGCTTTCTTCGCTTTTACCAGATCCGCCACTTTGGCTTTTCCGGCCAACGCGGc                                                                                         >  1:8180330/1‑94 (MQ=255)
    aTAATTTCTTCCAGTTTCGCGCCATTGCCAATAATTTTGGCTTTCTTCGCTTTTACCAGATCCGCCCTTTTGGCTTTTCCGGTCAGCGCGGCGt                                                                                       >  1:8253464/1‑94 (MQ=255)
     tAATTTCTTCCAGTTTCGCGCCATTGCCAATAATTTTGGCTTTCTTCGCTTTTACCAGATCCGCCATTTTGGCTTGTCCCGTCAGCgcggcggg                                                                                      >  1:4242121/1‑92 (MQ=255)
      aaTTTCTTCCAGTTTCGCGCCATTGCCAATAATTTTGGCTTTCTTCGCTTTTACCAGATCCGCCATTTTGGCTTGTCCGGTCCGCGCGGCGTGc                                                                                     >  2:10519319/1‑94 (MQ=255)
      aaTTTCTTCCAGTTTCGCGCCATTGCCAATAATTTTGGCTTTCTTCGCTTTTACCAGATCCGCCATTTTGGCTTGTCCGGTCAGCGCGGCGTGc                                                                                     >  1:1152200/1‑94 (MQ=255)
        tttCTTCCAGTTTCGCGCCATTGCCAATAATTTTGGCTTTCTTCGCTTTTACCAGATCCGCCATTTTGGCTTGTCCGGGCAGCGCGGCGTGCAg                                                                                   >  2:3231800/1‑94 (MQ=255)
         ttcttcCAGTTTCGCGCCATTGCCAATAATTTTGGCTTTCTTCGCTTTTACCAGATCCGCCATTTTGGCTTGTCCGGTCAGCGCGGCGTGCAGa                                                                                  >  1:1787847/1‑94 (MQ=255)
           cttcCAGTTTCGCGCCATTGCCAATAATTTTGGCTTTCTTCGCTTTTACCAGATCCGCCATTTTGGCTTGTCCGGTCAGCGCGGCGTGCAGATc                                                                                >  2:2353648/1‑94 (MQ=255)
                aGTTTCGCGCCATTGCCAATAATTTTGGCTTTCTTCGCTTTTACCAGATCCGCCATTTTGGCTTGTCCGGTCAGCGCGGCGTGCAGATCTTCAc                                                                           >  1:7365376/1‑94 (MQ=255)
                aGTTTCGCGCCATTGCCAATAATTTTGGCTTTCTTCGCTTTTACCAGATCCGCCATTTTGGCTTGTCCGGTCAGCGCGGCGTGCAGATCTTCAc                                                                           >  2:835144/1‑94 (MQ=255)
                 gTTTCGCGCCATTGCCAATAATTTTGGCTTTCTTCGCTTTTACCAGATCCGCCATTTTGGCTTGTCCGGTCAGCGCGGCGTGCAGATCTTCACg                                                                          >  1:10213733/1‑94 (MQ=255)
                 gTTTCGCGCCATTGCCAATAATTTTGGCTTTCTTCGCTTTTACCAGATCCGCCATTTTGGCTTGTCCGGTCAGCGCGGCGTGCAGATCTTCACg                                                                          >  1:6627658/1‑94 (MQ=255)
                 gTTTCGCGCCATTGCCAATAATTTTGGCTTTCTTCGCTTTTACCAGATCCGCCATTTTGGCTTGTCCGGTCAGCGCGGCGTGCAGATCTTCACg                                                                          >  1:5919048/1‑94 (MQ=255)
                  tttCGCGCCATTGCCAATAATTTTGGCTTTCTTCGCTTTTACCAGATCCGCCATTTTGGCTTGTCCGGTCAGCGCGGCGTGCAGATCTTCACgg                                                                         >  2:7227058/1‑94 (MQ=255)
                  tttCGCGCCATTGCCAATAATTTTGGCTTTCTTCGCTTTTACCAGATCCGCCATTTTGGCTTGTCCGGTCAGCGCGGCGTGCAGATCTTCACgg                                                                         >  2:5880102/1‑94 (MQ=255)
                     cgcgCCATTGCCAATAATTTTGGCTTTCTTCGCTTTTACCAGATCCGCCATTTTGGCTTGTCCGGTCAGCGCGGCGTGCAGATCTTCACGGCTg                                                                      >  2:25064/1‑94 (MQ=255)
                       cgcCATTGCCAATAATTTTGGCTTTCTTCGCTTTTACCAGATCCGCCATTTTGGCTTGTCCGGTCAGCGCGGCGTGCAGATCTTCACGGCTGAt                                                                    <  2:8897211/94‑1 (MQ=255)
                       cgcCATTGCCAATAATTTTGGCTTTCTTCGCTTTTACCAGATCCGCCATTTTGGCTTGTCCGGTCAGCGCGGCGTGCAGATCTTCACGGCTGAt                                                                    >  2:1780909/1‑94 (MQ=255)
                       cgcCATTGCCAATAATTTTGGCTTTCTTCGCTTTTACCAGATCCGCCATTTTGGCTTGTCCGGTCAGCGCGGCGTGCAGATCTTCACGGCTGAt                                                                    >  1:4097973/1‑94 (MQ=255)
                       cgcCATTGCCAATAATTTTGGCTTTCTTCGCTTTTACCAGATCCGCCATTTTGGCTTGTCCGGTCAGCGCGGCGTGCAGATCTTCACGGCTGAt                                                                    <  1:6301408/94‑1 (MQ=255)
                         ccATTGCCAATAATTTTGGCTTTCTTCGCTTTTACCAGATCCGCCATTTTGGCTTGTCCGGTCAGCGCGGCGTGCAGATCTTCACGGCTGATGt                                                                  >  2:3731057/1‑94 (MQ=255)
                          cATTGCCAATAATTTTGGCTTTCTTCGCTTTTACCAGATCCGCCATTTTGGCTTGTCCGGTCAGCGCGGCGTGCAGATCTTCACGGCTGATGTa                                                                 >  2:1404912/1‑94 (MQ=255)
                          cATTGCCAATAATTTTGGCTTTCTTAGCTTTTAACAGATCCGCCATTTTGGCTTGTCCGGTCAGCGCGTCGTGCAGCTCTTCACGGCTGATGtt                                                                 >  1:4637780/1‑93 (MQ=255)
                           aTTGCCAATAATTTTGGCTTTCTTCGCTTTTACCAGATCCGCCATTTTGGCTTGTCCGGTCAGCGCGGCGTGCAGATCTTCACGGCTGCTGTaa                                                                >  2:5652335/1‑93 (MQ=255)
                            ttGCCAATAATTTTGGCTTTCTTCGCTTTTACCAGATCCGCCATTTTGGCTTGTCCGGTCAGCGCGGCGTGCAGATCTTCACGGCTGCTGCAGa                                                               >  2:7146182/1‑94 (MQ=255)
                             tGCCAATAATTTTGGCTTTCTTCGCTTTTACCAGATCCGCCATTTTGGCTTGTCCGGTCAGCGCGGCGTGCAGATCTTCACGGCTGATGTAGaa                                                              >  2:8514562/1‑94 (MQ=255)
                             tGCCAATAATTTTGGCTTTCTTCGCTTTTACCAGATCCGCCATTTTGGCTTGTCCGGTCAGCGCGGCGTGCAGATCTTCACGGCTGATGTAGaa                                                              >  1:450658/1‑94 (MQ=255)
                             tGCCAATAATTTTGGCTTTCTTCGCTTTTACCAGATCCGCCATTTTGGCTTGTCCGGTCAGCGCGGCGTGCAGATCTTAACGGCTGATGTAgta                                                              >  1:1515184/1‑92 (MQ=255)
                                cAATAATTTTGGCTTTCTTCGCTTTTACCAGATCCGCCATTTTGGCTTGTCCGGTCAGCGCGGCGTGCAGATCTTCACGGCTGATGTAGAAaga                                                           >  2:4175182/1‑94 (MQ=255)
                                 aataatTTTGGCTTTCTTCGCTTTTACCAGATCCGCCATTTTGGCTTGTCCGGTCAGCGCGGCGTGCAGATCTTCACGGCTGATGTAGAAagag                                                          <  1:9051086/94‑1 (MQ=255)
                                 aataatTTTGGCTTTCTTCGCTTTTACCAGATCCGCCATTTTGGCTTGTCCGGTCAGCGCGGCGTGCAGATCTTCACGGCTGATGTAGAAagag                                                          >  2:4664808/1‑94 (MQ=255)
                                  ataatTTTGGCTTTCTTCGCTTTTACCAGATCCGCCATTTTGGCTTGTCCGGTCAGCGCGGCGTGCAGATCTTCACGGCTGATGTAGAAAGAgg                                                         >  2:10084128/1‑94 (MQ=255)
                                  ataatTTTGGATTTCTTCGCTTTTACCAGATCCGCCATTTTGGCTTGTCCGGTCAGCGCGGCGTGCCGATCTTCGCGGCTGATGTAGAAAGAgg                                                         >  2:3639108/1‑94 (MQ=255)
                                    aatTTTGGCTTTCTTCGCTTTTACCAGATCCGCCATTTTGGCTTGTCCGGTCAGCGCGGCGTGCAGATCTTCACGGCTGATGTAGAAAGAGGCg                                                       >  2:2204945/1‑94 (MQ=255)
                                     atTTTGGCTTTCTTCGCTTTTACCAGATCCGCCATTTTGGCTTGTCCGGTCAGCGCGGCGTGCAGATCTTCACGGCTGATGTAGGAAGAggggc                                                      >  2:4253647/1‑91 (MQ=255)
                                      ttttGGCTTTCTTCGCTTTTACCAGATCCGCCATTTTGGCTTGTCCGGTCAGCGCGGCGTGCAGATCTTCACGGCTGATGTAGAAAGAGGGGTc                                                     >  2:7746114/1‑94 (MQ=255)
                                      ttttGGCTTTCTTCGCTTTTACCAGATCCGCCATTTTGGCTTGTCCGGTCAGCGCGGCGTGCAGATCTTCACGGCTGATGTAGAAAGAGGCGTc                                                     >  1:7764883/1‑94 (MQ=255)
                                       tttGGCTTTCTTCGCTTTTACCAGATCCGCCATTTTGGCTTGTCCGGTCAGCGCGGCGTGCAGATCTTCACGGCTGATGTAGAAAGAGGCGTCg                                                    >  1:1648813/1‑94 (MQ=255)
                                       tttGGCTTTCTTCGCTTTTACCAGATCCGCCATTTTGGCTTGTCCGGTCAGCGCGGCGTGCAGATCTTCACGGCTGATGTAGAAAGAGGCGTCg                                                    >  1:6878448/1‑94 (MQ=255)
                                       tttGGCTTTCTTCGCTTTTACCAGATCCGCCATCTTGGCTTGTCCGGTCAGCGCGGCGTGCAGATCTTCACGGCTGATGTAGAAAGAGgcggcg                                                    >  1:3775596/1‑94 (MQ=255)
                                        ttGGCTTTCTTCGCTTTTACCAGATCCGCCATTTTGGCTTGTCCGGTCAGCGCGGCGTGCAGATCTTCACGGCTGATGTAGAAAGAGGcggcgg                                                   >  1:2283924/1‑94 (MQ=255)
                                         tGGCTTTCTTCGCTTTTACCAGATCCGCCATTTTGGCTTGTCCGGTCAGCGCGGCGTGCAGATCTTCACGGCTGATGTAGAAAGAGGCGTCGGc                                                  >  2:7502617/1‑94 (MQ=255)
                                           gCTTTGTTCGCTTTAACCAGATCCGCCATTTTGGCTTGTCCGGTCAGCGCGGCGTGCAGATCTTCACGGCTGATGTAGAAAGAGGCGGCGGCtt                                                <  2:7988136/94‑1 (MQ=255)
                                             tttCTTCGCTTTTACCAGATCCGCCATTTTGGCTTGTCCGGTCAGCGCGGCGTGCAGATCTTCACGGCTGCTGTAGAAAGAGGCGTCGGCTTGc                                              >  2:4846903/1‑94 (MQ=255)
                                             tttCTTCGCTTTTACCAGATCCGCCATTTTGGCTTGTCCGGTCAGCGCGGCGTGCAGATCTTCACGGCTGATGTAGAAAGAGGCGTCGGCTTGc                                              >  2:7422907/1‑94 (MQ=255)
                                              ttcttcGCTTTTACCAGATCCGCCATTTTGGCTTGTTCGGTCAGCGCGGCGTGCAGATCTTCACGGCTGGTGTAGAAAGAGGCGTCGGCTTGC‑g                                            >  2:4105805/1‑94 (MQ=255)
                                                cttcGCTTTTACCAGATCCGCCATTTTGGCTTGTCCGGTCAGCGCGGCGTGCAGATCTTCACGGCTGATGTAGAAAGAGGCGTCGGCTTGC‑GGt                                          <  1:8977966/94‑1 (MQ=255)
                                                cttcGCTTTTACCAGATCCGCCATTTTGGCTTGTCCGGTCAGCACGGCGTGCAGATCTTCACGGCTGATTTAGAAAGAGGCGGCGGCTTTC‑GGt                                          >  2:6989078/1‑94 (MQ=255)
                                                  tcGCTTTTACCAGATCCGCCATTTTGGCTTGTCCGGTCAGCGCGGCGTGCAGATCTTCACGGCTGATGTAGAAAGAGGCGTCGGCTTGC‑GGCTg                                        >  2:5280552/1‑94 (MQ=255)
                                                    gCTTTTACCAGATCCGCCATTTTGGCTTGTCCGGTCAGCGCGGCGTGCAGATCTTCACGGCTGATGTAGAAAGAGGCGTCGGCTTGC‑TGTTGCa                                      >  2:3932379/1‑94 (MQ=255)
                                                    gCTTTTACCAGATCCGCCATTTTGGCTTGTCCGGTCAGCGCGGCGTGCAGATCTTCACGGCTGATGTAGAAAGAGGCGTCGGCTTGC‑GGTTGCa                                      >  2:10382615/1‑94 (MQ=255)
                                                     cTTTTACCAGATCCGCCATTTTGGCTTGTCCGGTCAGCGCGGCGTGCAGATCTTCACGGCTGATGTAGAAAGAGGCGTCGGCTTGC‑GGGTGCAg                                     >  2:9914996/1‑94 (MQ=255)
                                                     cTTTTACCAGATCCGCCATTTTGGCTTGTCCGGTCAGCGCGGCGTGCAGATCTTCACGGCTGAGGTGGAAAGAGGCGTCGGCGTGC‑GGTTggcg                                     >  1:3555382/1‑91 (MQ=255)
                                                       tttACCAGATCCGCCATTTTGGCTTGTCCGGTCAGCGCGGCGTGCAGATCTTCACGGCTGATGTAGAAAGAGGCGGCGGGTTGC‑GGTTGCcgcg                                   >  1:2556792/1‑94 (MQ=255)
                                                        ttACCAGATCCGCCATTTTGGCTTGTCCGGTCAGCGCGGCGTGCAGATCTTCACGGCTGATGTAGGAAGAGGCGTCGGCCTGCTGGTTG‑AGCGt                                  >  1:5678992/1‑94 (MQ=255)
                                                        ttACCAGATCCGCCATTTTGGCTTGTCCGGTCAGCGCGGCGTGCAGATCTTCACGGCTGATGTAGAAAGAGGCGTCGGCTTGC‑GGTTGCGGCGt                                  >  1:7061900/1‑94 (MQ=255)
                                                        ttACCAGATCCGCCATTTTGGCTTGTCCGGTCAGCGCGGCGTGCAGATCTTCACGGCTGATGTAGAAAGAGGCGTCGGCTTGC‑GGTTGCAGCGt                                  >  2:7917917/1‑94 (MQ=255)
                                                         tACCAGATCCGCCATTTTGGCTTGTCCGGTCAGCGCGGCGTGCAGATCTTCACGGCTGATGTAGAAAGAGGCGTCGGCTTGC‑GGTTTCcgcggt                                 >  1:9228156/1‑92 (MQ=255)
                                                         tACCAGATCCGCCATTTTGGCTTGTCCGGTCAGCGCGGCGTGCAGATCTTCACGGCTGATGTAGAAAGAGGCGTCGGCTTGC‑GGTTGCAGCGtt                                 >  1:8479454/1‑94 (MQ=255)
                                                          aCCAGATCCGCCATTTTGGCTTGTCCGGTCAGCGCGGCGTGCAGATCTTCACGGCTGATGTAGAAAGAGGCGTCGGCTTGC‑GGTTGCAGCGttt                                >  2:9049762/1‑94 (MQ=255)
                                                          aCCAGATCCGCCATTTTGGCTTGTCCGGTCAGCGCGGCGTGCAGATCTTCACGGCTGATGTAGAAAGAGGCGTCGGCTTGC‑GGTTGCAGCGttt                                >  1:3700728/1‑94 (MQ=255)
                                                          aCCAGATCCGCCATTTTGGCTTGTCCGGTCAGCGCGGCGTGCAGATCTTCACGGCTGATGTAGAAAGAGGCGTCGGCTTGC‑GGTTGCAGCGttt                                >  2:1141757/1‑94 (MQ=255)
                                                          aCCAGATCCGCCATTTTGGCTTGTCCGGTCAGCGCGGCGTGCAGATCTTCACGGCTGATGTAGAAAGAGGCGTCGGCCTTG‑GGGTGCAGCCttt                                >  2:3333855/1‑94 (MQ=255)
                                                           ccAGATCCGCCATTTTGGCTTGTCCGGTCAGCGCGGCGTGCAGATCTTCACGGCTGATGTAGAAAGAGGCGTCGGCTTGC‑GGTTGCAGCGtttt                               <  2:5155686/94‑1 (MQ=255)
                                                             aGATCCGCCATTTTGGCTTGTCCGGTCAGCGCGGCGTGCAGATCTTCACGGCTGATGTAGAAAGAGGCGTCGGCTTGC‑GGTTGCAGGGTTTTcc                             >  2:8646130/1‑94 (MQ=255)
                                                             aGATCCGCCATTTTGGCTTGTCCGGTCAGCGCGGCGTGCAGATCTTCACGGCTGATGTAGAAAGAGGCGTCGGCTTGC‑GGTTGCAGCGTTTTcc                             >  1:9590651/1‑94 (MQ=255)
                                                              gATCCGCCATTTTGGCTTGTCCGGTCAGCGCGGCGTGCAGATCTTCACGGCTGATGTAGAAAGAGGCGTCGGCTTGG‑GGTTGCAGGGTTTTCCg                            >  1:10457117/1‑94 (MQ=255)
                                                               aTCCGCCATTTTGGCTTGTCCGGTCAGCGCGGCGTGCAGATCTTCACGGCTGATGTAGAAAGAGGCGTCGGCTTGC‑GGTTGCAGTGTTTTCCgg                           >  2:8201412/1‑94 (MQ=255)
                                                               aTCCGCCATTTTGGCTTGTCCGGTCAGCGCGGCGTGCAGATCTTCACGGCTGATGTAGAAAGAGGCGTCGGCTTGC‑GGTTGCAGCGGTTTCCgg                           >  1:2927525/1‑94 (MQ=255)
                                                                tCCGCCATTTTGGCTTGTCCGGTCAGCGCGGCGTGCAGATCTTCACGGCTGATGTAGAAAGAGGCGTCGGCTTGC‑GGTTGCAGCGTTTTCCGGt                          <  1:8135259/94‑1 (MQ=255)
                                                                tCCGCCATTTTGGCTTGTCCGGTCAGCGCGGCGTGCAGATCTTCACGGCTGATGTAGAAAGAGGCGTCGGCTTGC‑GGGTGCAGCGTTTTCCGGt                          >  1:6103309/1‑94 (MQ=255)
                                                                 ccGCCATTTTGGCTTGTCCGGTCAGCGCGGCGTGCAGATCTTCACGGCTGATGTAGAAAGAGGCGTCGGCTTGC‑GGTTGCAGCGTTTTCCggga                         >  2:2792236/1‑92 (MQ=255)
                                                                   gCCATTTTGGCTTGTCCGGTCAGCGCGGCGTGCAGATCTTCACGGCTGATGTAGAAAGAGGCGTCGGCTTGC‑GGTTGCAGCGTTTTCCGGTAGt                       >  1:2641077/1‑94 (MQ=255)
                                                                   gCCATTTTGGCTTGTCCGGTCAGCGCGGCGTGCAGATCTTCACGGCTGATGTAGAAAGAGGCGTCGGCTTGC‑GGTTGCAGCGTTTTCCGGTAGt                       >  2:9771503/1‑94 (MQ=255)
                                                                    ccATTTTGGCTTGTCCGGTCAGCGCGGCGTGCAGATCTTCACGGCTGATGTAGAAAGAGGCGTCGGCTTGC‑GGGTGCAGCGTTTTCCGGTAGtt                      >  1:7604104/1‑94 (MQ=255)
                                                                     cATTTTGGCTTGTCCGGTCAGCGCGGCGTGCAGATCTTCACGGCTGATGTAGAAAGAGGCGTCGGCTTGC‑GGTTGCAGCGTTTTCCGGTAGTTa                     >  1:6287029/1‑94 (MQ=255)
                                                                      aTTTTGGCTTGTCCGGTCAGCGCGGCGTGCAGATCTTCACGGCTGATGTAGAAAGAGGCGTCGGCTTGC‑GGTTGCAGCGTTTTCCGGTAGTTaa                    >  2:10014280/1‑94 (MQ=255)
                                                                      aTTTTGGCTTGTCCGGTCAGCGCGGCGTGCAGATCTTCACGGCTGATGTAGAAAGAGGCGTCGGCTTGC‑GGTTGCAGCGTTTTCCGGTAGTTaa                    <  1:8768818/94‑1 (MQ=255)
                                                                      aTTTTGGCTTGTCCGGTCAGCGCGGCGTGCAGATCTTCACGGCTGATGTAGAAAGAGGCGTCGGCTTGC‑GGTTGCAGCGTTTTCCGGTAGTTaa                    <  1:4772335/94‑1 (MQ=255)
                                                                      aTTTTGGCTTGTCCGGTCAGCGCGGCGTGCAGATCTTCACGGCTGATGTAGAAAGAGGCGTCGGCTTGC‑GGTTGCAGCGTTTTCCGGTAGTTaa                    <  2:9247395/94‑1 (MQ=255)
                                                                         ttGGCTTGTCCGGTCAGCGCGGCGTGCAGATCTTCACGGCTGATGTAGAAAGAGGCGTCGGCTTGC‑GGTTGCAGCGTTTTCCGGTAGTTAAGCa                 >  2:7561342/1‑94 (MQ=255)
                                                                         ttGGCTTGTCCGGTCAGCGCGGCGTGCAGATCTTCACGGCTGATGTAGAAAGAGGCGTCGGCTTGC‑GGTTGCAGCGTTTTCCGGTAGTTAAGCa                 <  2:6520647/94‑1 (MQ=255)
                                                                          tGGCTTGTCCGGTCAGCGCGGCGTGCAGATCTTCACGGCTGATGTAGAAAGAGGCGTCGGCTTGC‑GGTTGCAGCGTTTTCCGGTAGTTAAGCAc                <  2:6283421/94‑1 (MQ=255)
                                                                           ggCTTGTCCGGTCAGCGCGGCGTGCAGATCTTCACGGCTGATGTAGAAAGAGGCGTCGGCTTGC‑GGTTGCAGCGTTTTCCGGTAGTTAAGCACg               >  1:8828978/1‑94 (MQ=255)
                                                                            gCTTGTCCGGTCAGCGCGGCGTGCAGATCTTCACGGCTGATGTAGAAAGAGGCGTCGGCTTGC‑GGTTGCAGCGTTTTCCGGTAGTTAAGCACGc              >  2:1281325/1‑94 (MQ=255)
                                                                             cTTGTCCGGTCAGCGCGGCGTGCAGATCTTCACGGCTGATGTAGAAAGAGGCGTCGGCTTGC‑GGTTGCAGCGTTTTCCGGTAGTTAAGCACGCt             <  2:9348775/94‑1 (MQ=255)
                                                                             cTTGTCCGGTCAGCGCGGCGTGCAGATCTTCACGGCTGATGTAGAAAGAGGCGTCGGCTTGC‑GGTTGCAGCGTTTTCCGGTAGTTAAGCACGCt             <  1:10106745/94‑1 (MQ=255)
                                                                              ttGTCCGGTCAGCGCGGCGTGCAGATCTTCACGGCTGATGTAGAAAGAGGCGTCGGCTTGC‑GGTTGCAGCGTTTTCCGGTAGTTAAGCACGCTa            >  2:6642997/1‑94 (MQ=255)
                                                                               tGTCCGGTCAGCGCGGCGTGCAGATCTTCACGGCTGATGTAGAAAGAGGCGTCGGCTTGC‑GGTTGCAGCGTTTTCCGGTAGTTAAGCACGCTAt           >  2:4425908/1‑94 (MQ=255)
                                                                               tGTCCGGTCAGCGCGGCGTGCAGATCTTCACGGCTGATGTAGAAAGAGGCGTCGGCTTGC‑GGTTGCAGCGTTTTCCGGTAGGTAAGGACGCTAt           >  1:3118197/1‑94 (MQ=255)
                                                                               tGTCCGGTCAGCGCGGCGTGCAGATCTTCACGGCTGATGTAGAAAGAGGCGTCCGCTTGC‑GGTTGCAGCGTTTTCCGGTAGTTAAGCACGCTAt           >  2:5969626/1‑94 (MQ=255)
                                                                                gTCCGGTCAGCGCGGCGTGCAGATCTTCACGGCTGATGTAGAAAGAGGCGTCGGCTTGC‑GGTTGCAGCGTTTTCCGGTAGTTAAGCACGCTATc          >  2:10266068/1‑94 (MQ=255)
                                                                                gTCCGGTCAGCGCGGCGTGCAGATCTTCACGGCTGATGTAGAAAGAGGCGTCGGCTTGC‑GGCTGCAGCGTTTTCCGGTAGTTAAGCACGCTATc          >  2:1572934/1‑94 (MQ=255)
                                                                                 gccGGTCAGCGCGGCGTGCAGATCTTCACGGCTGATGTAGAAAGAGGCGTCGGCTTGC‑GGTTGCAGCGTTTTCCGGTAGTTAAGCACGCTATCg         <  2:2062034/93‑1 (MQ=255)
                                                                                   cGGTCAGCGCGGCGTGCAGATCTTCACGGCTGATGTAGAAAGAGGCGTCGGCTTGC‑GGTTGCAGCGTTTTCCGGTAGTTAAGCACGCTATCGtt       >  1:327354/1‑94 (MQ=255)
                                                                                    ggTCAGCGCGGCGTGCAGATCTTCACGGCTGATGTAGAAAGAGGCGTCGGCTTGC‑GGTTGCAGCGTTTTCCGGTAGTTAAGCACGCTATCGTTc      <  1:5343182/94‑1 (MQ=255)
                                                                                     gTCAGCGCGGCGTGCAGATCTTCACGGCTGATGTAGAAAGAGGCGTCGGCTTGC‑GGTTGCAGCGTTTTCCGGTAGTTAAGCACGCTATCGTTCa     <  1:1009559/94‑1 (MQ=255)
                                                                                      tCAGCGCGGCGTGCAGATCTTCACGGCTGATGTAGAAAGAGGCGTCGGCTTGC‑GGTTGCAGCGTTTTCCGGTAGTTAAGCACGCTATCGTTCAg    <  2:1690232/94‑1 (MQ=255)
                                                                                           |                                                                                           
GGCGATAATTTCTTCCAGTTTCGCGCCATTGCCAATAATTTTGGCTTTCTTCGCTTTTACCAGATCCGCCATTTTGGCTTGTCCGGTCAGCACGGCGTGCAGATCTTCACGGCTGATGTAGAAAGAGGCGTCGGCTTGC‑GGTTGCAGCGTTTTCCGGTAGTTAAGCACGCTATCGTTCAGCG  >  NC_000913/4302239‑4302420

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 17 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output Only 200 of 315 total aligned reads displayed.
GAGTAATGATTAATGTGGATAGAGTTTCTTTTTGAGGTTGGCTAATGAAACGCTTTCCGCTATTTCTTTTATTCACCCTGCTCACGCTGTCC‑A‑C‑GTTCCGGCCCAGGCCGATATTATCGACGACACCATTGGCAATATTCAGCAGGCGATTAATGACGCTTATAACCCTGACCGTGGGCGAGACTAT  >  NC_000913/4311403‑4311589
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GAGTAATGATTAATGTGGATAGAGTTTCTTTTTGAGGTTGGCTAATGAAACGCTTTCCGCTATTTCTTTTATTCACCCTGCTCACGCTGTCC‑A‑C                                                                                                >  SRR4302545.5878875/1‑94 (MQ=60)
 AGTAATGATTAATGTGGATAGAGTTTCTTTTTGAGGTTGGCTAATGAAACGCTTTCCGCTATTTCTTTTATTCACCCTGCTCACGCTGTCC‑A‑C‑C                                                                                              >  SRR4302545.3457188/1‑94 (MQ=60)
 AGTAATGATTAATGTGGATAGAGTTTCTTTTTGAGGTTGGCTAATGAAACGCTTTCCGCTATTTCTTTTATTCACCCTGCTCACGCTGTCC‑A‑C‑C                                                                                              >  SRR4302545.9184504/1‑94 (MQ=60)
  GTAATGATTAATGTGGATAGAGTTTCTTTTTGAGGTTGGCTAATGAAACGCTTTCCGCTATTTCTTTTATTCACCCTGCTCACGCTGTCC‑A‑Ccg                                                                                              <  SRR4302545.9570930/94‑3 (MQ=60)
  GTAATGATTAATGTGGATAGAGTTTCTTTTTGAGGTTGGCTAATGAAACGCTTTCCGCTATTTCTTTTATTCACCCTGCTCACGCTGTCC‑A‑Ccg                                                                                              <  SRR4302545.9429375/94‑3 (MQ=60)
  GTAATGATTAATGTGGATAGAGTTTCTTTTTGAGGTTGGCTAATGAAACGCTTTCCGCTATTTCTTTTATTCACCCTGCTCACGCTGTCC‑A‑Ccg                                                                                              >  SRR4302545.10486885/1‑92 (MQ=60)
   TAATGATTAATGTGGATAGAGTTTCTTTTTGAGGTTGGCTAATGAAACGCTTTCCGCTATTTCTTTTATTCACCCTGCTCACGCTGTCC‑A‑Ccgt                                                                                             >  SRR4302545.1128710/1‑91 (MQ=60)
   TAATGATTAATGTGGATAGAGTTTCTTTTTGAGGTTGGCTAATGAAACGCTTTCCGCTATTTCTTTTATTCACCCTGCTCACGCTGTCC‑A‑Ccgt                                                                                             >  SRR4302545.1902268/1‑91 (MQ=60)
    AATGATTAATGTGGATAGAGTTTCTTTTTGAGGTTGGCTAATGAAACGCTTTCCGCTATTTCTTTTATTCACCCTGCTCACGCTGTCC‑ACC‑GTT                                                                                            <  SRR4302545.1237131/94‑1 (MQ=60)
    AATGATTAATGTGGATAGAGTTTCTTTTTGAGGTTGGCTAATGAAACGCTTTCCGCTATTTCTTTTATTCACCCTGCTCACGCTGTCC‑ACC‑GTT                                                                                            <  SRR4302545.4096321/94‑1 (MQ=60)
    AATGATTAATGTGGATAGAGTTTCTTTTTGAGGTTGGCTAATGAAACGCTTTCCGCTATTTCTTTTATTCACCCTGCTCACGCTGTCC‑ACC‑GTT                                                                                            <  SRR4302545.82649/94‑1 (MQ=60)
      TGATTAATGTGGATATAGTTTCTTTTTGAGGTTGGCTAATGAAACGCTTTCCGCTATTTCTTTTATTCACCCTGCTCAGGCTGTCC‑ACC‑GTTCC                                                                                          <  SRR4302545.1577370/94‑1 (MQ=60)
      TGATTAATGTGGATAGAGTTTCTTTTTGAGGTTGGCTAATGAAACGCTTTCCGCTATTTCTTTTATTCACCCTGCTCACGCTGTCC‑ACC‑GTTCC                                                                                          >  SRR4302545.896503/1‑94 (MQ=60)
      TGATTAATGTGGATAGAGTTTCTTTTTGAGGTTGGCTAATGAAACGCTTTCCGCTATTTCTTTTATTCACCCTGCTCACGCTGTCC‑ACC‑GTTCC                                                                                          <  SRR4302545.9493246/94‑1 (MQ=60)
       GATTAATGTGGATAGAGTTTCTTTTTGAGGTTGGCTAATGAAACGCTTTCCGCTATTTCTTTTATTCACCCTGCTCACGCTGTCC‑ACC‑GTTCCG                                                                                         >  SRR4302545.6654827/1‑94 (MQ=60)
       GATTAATGTGGATAGAGTTTCTTTTTGAGGTTGGCTAATGAAACGCTTTCCGCTATTTCTTTTATTCACCCTGCTCACGCTGTCC‑ACC‑GTTCCG                                                                                         <  SRR4302545.3154796/94‑1 (MQ=60)
       GATTAATGTGGATAGAGTTTCTTTTTGAGGTTGGCTAATGAAACGCTTTCCGCTATTTCTTTTATTCACCCTGCTCACGCTGTCC‑ACC‑GTTCCG                                                                                         <  SRR4302545.2905793/94‑1 (MQ=60)
        ATTAATGTGGATAGAGTTTCTTTTTGAGGTTGGCTAATGAAACGCTTTCCGCTATTTCTTTTATTCACCCTGCTCACGCTGTCC‑A‑Ccgttgcgg                                                                                        >  SRR4302545.9166442/1‑86 (MQ=60)
           AATGTGGATAGAGTTTCTTTTTGAGGTTGGCTAATGAAACGCTTTCCGCTATTTCTTTTATTCACCCTGCTCACGCTGTCC‑ACC‑GTTCCGGCCC                                                                                     >  SRR4302545.2115278/1‑94 (MQ=60)
           AATGTGGATAGAGTTTCTTTTTGAGGTTGGCTAATGAAACGCTTTCCGCTATTTCTTTTATTCACCCTGCTCACGCTGTCC‑ACC‑GTTCCGGCCC                                                                                     <  SRR4302545.700762/94‑1 (MQ=60)
            ATGTGGATAGAGTTTCTTTTTGAGGTTGGCTAATGAAACGCTTTCCGCTATTTCTTTTATTCACCCTGCTCACGCTGTCC‑ACC‑GTTCCGGCCCG                                                                                    >  SRR4302545.1489945/1‑94 (MQ=60)
            ATGTGGATAGAGTTTCTTTTTGAGGTTGGCTAATGAAACGCTTTCCGCTATTTCTTTTATTCACCCTGCTCACGCTGTCC‑ACC‑GTTCCGGCCCA                                                                                    <  SRR4302545.977345/94‑1 (MQ=60)
            ATGTGGATAGAGTTTCTTTTTGAGGTTGGCTAATGAAACGCTTTCCGCTATTTCTTTTATTCACCCTGCTCACGCTGTCC‑ACC‑GTTCCGGCCCA                                                                                    >  SRR4302545.81506/1‑94 (MQ=60)
            ATGTGGATAGAGTTTCTTTTTGAGGTTGGCTAATGAAACGCTTTCCGCTATTTCTTTTATTCACCCTGCTCACGCTGTCC‑ACC‑GTTCCGGCCCA                                                                                    >  SRR4302545.801207/1‑94 (MQ=60)
             TGTGGATAGAGTTTCTTTTTGAGGTTGGCTAATGAAACGCTTTCCGCTATTTCTTTTATTCACCCTGCTCACGCTGTCC‑ACC‑GTTCCGGCCCAG                                                                                   >  SRR4302545.1229267/1‑94 (MQ=60)
              GTGGATAGAGTTTCTTTTTGAGGTTGGCTAATGAAACGCTTTCCGCTATTTCTTTTATTCACCCTGCTCACGCTGTCC‑ACC‑GTTCCGGCCCAGG                                                                                  >  SRR4302545.4675567/1‑94 (MQ=60)
              GTGGATAGAGTTTCTTTTTGAGGTTGGCTAATGAAACGCTTTCCGCTATTTCTTTTATTCACCCTGCTCACGCTGTCC‑ACC‑GTTCCGGCCCAGG                                                                                  <  SRR4302545.5033443/94‑1 (MQ=60)
               TGGATAGAGTTTCTTTTTGAGGTTGGCTAATGAAACGCTTTCCGCTATTTCTTTTATTCACCCTGCTCACGCTGTCC‑ACC‑GTTCCGGCCCAGGC                                                                                 >  SRR4302545.2688453/1‑94 (MQ=60)
               TGGATAGAGTTTCTTTTTGAGGTTGGCTAATGAAACGCTTTCCGCTATTTCTTTTATTCACCCTGCTCACGCTGTCC‑ACC‑GTTCCGGCCCAGGC                                                                                 >  SRR4302545.748826/1‑94 (MQ=60)
                GGATAGAGTTTCTTTTTGAGGTTGGCTAATGAAACGCTTTCCGCTATTTCTTTTATTCACCCTGCTCACGCTGTCC‑ACC‑GTTCCGGCCCAGGCC                                                                                >  SRR4302545.3266835/1‑94 (MQ=60)
                GGATAGAGTTTCTTTTTGAGGTTGGCTAATGAAACGCTTTCCGCTATTTCTTTTATTCACCCTGCTCACGCTGTCC‑ACC‑GTTCCGGCCCAGGCC                                                                                <  SRR4302545.3615307/94‑1 (MQ=60)
                GGATAGAGTTTCTTTTTGAGGTTGGCTAATGAAACGCTTTCCGCTATTTCTTTTATTCACCCTGCTCACGCTGTCC‑ACC‑GTTCCGGCCCAGGCC                                                                                <  SRR4302545.5752297/94‑1 (MQ=60)
                 GATAGAGTTTCTTTTTGAGGTTGGCTAATGAAACGCTTTCCGCTATTTCTTTTATTCACCCTGCTCACGCTGTCC‑ACC‑GTTCCGGCCCAGGCCG                                                                               <  SRR4302545.5864778/94‑1 (MQ=60)
                  ATAGAGTTTCTTTTTGAGGTTGGCTAATGAAACGCTTTCCGCTATTTCTTTTATTCACCCTGCTCACGCTGTCC‑ACC‑GTTCCGGCCCAGGGCGA                                                                              >  SRR4302545.4936632/1‑94 (MQ=60)
                   TATAGTTTCTTTTTGAGGTTGGCTAATGAAACGCTTTCCGCTATTTCTTTTATTCTCCCTGCTCACGCTGTCC‑ACC‑GTTCCGGCCCAGGCCGAT                                                                             <  SRR4302545.8416109/94‑1 (MQ=60)
                   TAGAGTTTCTTTTTGAGGTTGGCTAATGAAACGCTTTCCGCTATTTCTTTTATTCACCCTGCTCACGCTGTCC‑ACC‑GTTCCGGCCCAGGCCGAT                                                                             <  SRR4302545.10140731/94‑1 (MQ=60)
                   TAGAGTTTCTTTTTGAGGTTGGCTAATGAAACGCTTTCCGCTATTTCTTTTATTCACCCTGCTCACGCTGTCC‑ACC‑GCTCCGGCCCAGGCCGAT                                                                             >  SRR4302545.6208762/1‑94 (MQ=60)
                    AGAGTTTCTTTTTGAGGTTGGCTAATGAAACGCTTTCCGCTATTTCTTTTATTCACCCTGCTCACGCTGTCC‑ACC‑GTTCCGGCCCAGGCCGATA                                                                            <  SRR4302545.5091316/94‑1 (MQ=60)
                    AGAGTTTCTTTTTGAGGTTGGCTAATGAAACGCTTTCCGCTATTTCTTTTATTCACCCTGCTCACGCTGTCC‑ACC‑GTTCCGGCCCAGGCCGATA                                                                            >  SRR4302545.3659092/1‑94 (MQ=60)
                    AGAGTTTCTTTTTGAGGTTGGCTAATGAAACGCTTTCCGCTATTTCTTTTATTCACCCTGCTCACGCTGTCC‑ACC‑GTTCCGGCCCAGGCCGATA                                                                            <  SRR4302545.9844026/94‑1 (MQ=60)
                     GAGTTTCTTTTTGAGGTTGGCTAATGAAACGCTTTCCGCTATTTCTTTTATTCACCCTGCTCACGCTGTCC‑ACC‑GTTCCGGCCCAGGCCGATAT                                                                           >  SRR4302545.9182392/1‑94 (MQ=60)
                      AGTTTCTTTTTGAGGTTGGCTAATGAAACGCTTTCCGCTATTTCTTTTATTCACCCTGCTCACGCTGTCC‑ACC‑GTTCCGGCCCAGGCCGATATT                                                                          <  SRR4302545.2179194/94‑1 (MQ=60)
                       GTTTCTTTTTGAGGTTGGCTAATGAAACGCTTTCCGCTATTTCTTTTATTCACCCTGCTCACGCTGTCC‑ACC‑GTTCCGGCCCAGGCCGATATTA                                                                         >  SRR4302545.10087016/1‑94 (MQ=60)
                       GTTTCTTTTTGAGGTTGGCTAATGAAACGCTTTCCGCTATTTCTTTTATTCACCCTGCTCACGCTGTCC‑ACC‑GTTCCGGCCCAGGCCGATATTA                                                                         <  SRR4302545.10567285/94‑1 (MQ=60)
                        TTTCTTTTTGAGGTTGGCTAATGAAACGCTTTCCGCTATTTCTTTTATTCACCCTGCTCACGCTGTCC‑ACC‑GTTCCGGCCCAGGCCGATATTAT                                                                        <  SRR4302545.3200378/94‑1 (MQ=60)
                        TTTCTTTTTGAGGTTGGCTAATGAAACGCTTTCCGCTATTTCTTTTATTCACCCTGCTCACGCTGTCC‑ACC‑GTTCCGGCCCAGGCCGATATTAT                                                                        <  SRR4302545.271819/94‑1 (MQ=60)
                         TTCTTTTTGAGGTTGGCTAATGAAACGCTTTCCGCTATTTCTTTTATTCACCCTGCTCACGCTGTCC‑ACC‑GTTCCGGCCCAGGCCGATATTATC                                                                       <  SRR4302545.8890827/94‑1 (MQ=60)
                         TTCTTTTTGAGGTTGGCTAATGAAACGCTTTCCGCTATTTCTTTTATTCACCCTGCTCACGCTGTCC‑ACC‑GTTCCGGCCCAGGCCGATATTATC                                                                       <  SRR4302545.10318757/94‑1 (MQ=60)
                         TTCTTTTTGAGGTTGGCTAATGAAACGCTTTCCGCTATTTCTTTTATTCACCCTGCTCACGCTGTCC‑ACC‑GTTCCGGCCCAGGCCGATATTATC                                                                       >  SRR4302545.7934949/1‑94 (MQ=60)
                         TTCTTTTTGAGGTTGGCTAATGAAACGCTTTCCGCTATTTCTTTTATTCACCCTGCTCACGCTGTCC‑ACC‑GTTCCGGCCCAGGCCGATATTATC                                                                       >  SRR4302545.9304363/1‑94 (MQ=60)
                         TTCTTTTTGAGGTTGGCTAATGAAACGCTTTCCGCTATTTCTTTTATTCACCCTGCTCACGCTGTCC‑ACC‑GTTCCGGCCCAGGCCGATATTATC                                                                       >  SRR4302545.1391000/1‑94 (MQ=60)
                          TCTTTTTGAGGTTGGCTAATGAAACGCTTTCCGCTATTTCTTTTATTCACCCTGCTCACGCTGTCC‑ACC‑GTTCCGGCCCAGGCCGATATTATCG                                                                      <  SRR4302545.7275163/94‑1 (MQ=60)
                          TCTTTTTGAGGTTGGCTAATGAAACGCTTTCCGCTATTTCTTTTATTCACCCTGCTCACGCTGTCC‑ACC‑GTTCCGGCCCAGGCCGATATTATCG                                                                      <  SRR4302545.2831445/94‑1 (MQ=60)
                            TTTTTGAGGTTGGCTAATGAAACGCTTTCCGCTATTTCTTTTATTCACCCTGCTCACGCTGTCC‑ACC‑GTTCCGGCCCAGGCCGATATTATCGAC                                                                    <  SRR4302545.7747823/94‑1 (MQ=60)
                            TTTTTGAGGTTGGCTAATGAAACGCTTTCCGCTATTTCTTTTATTCACCCTGCTCACGCTGTCC‑ACC‑GTTCCGGCCCAGGCCGATATTATCGAC                                                                    <  SRR4302545.7086299/94‑1 (MQ=60)
                            TTTTTGAGGTTGGCTAATGAAACGCTTTCCGCTATTTCTTTTATTCACCCTGCTCACGCTGTCC‑ACC‑GTTCCGGCCCAGGCCGATATTATCGAC                                                                    >  SRR4302545.2616105/1‑94 (MQ=60)
                            TTGTTGAGGTTGACTAATGAGACGCTTACCGCTATTTCTTTTATTCACCTTCCTCACCCGGTCCTG‑C‑GTTCCGGCCCAGGCCGATATTATCGGC                                                                    <  SRR4302545.1188011/94‑1 (MQ=36)
                             TTTTGAGGTTGGTTAATGAAACGCTTTCCGCTATTTCTTTTATTCCCCCTGCTCACGCTGTCC‑ACC‑GTTCCGGCCCAGGCCGATATTCTCGACG                                                                   <  SRR4302545.4074286/94‑1 (MQ=60)
                             TTTTGAGGTTGGCTAATGAAACGCTTTCCGCTATTTCTTTTATTCACCCTGCTCACGCTGTCC‑ACC‑GTTCCGGCCCAGGCCGATATTATCGACG                                                                   >  SRR4302545.2413869/1‑94 (MQ=60)
                             TTTTGAGGTTGGCTAATGAAACGCTTTCCGCTATTTCTTTTATTCACCCTGCTCACGCTGTCC‑ACC‑GTTCCGGCCCAGGCCGATATTATCGACG                                                                   <  SRR4302545.3753860/94‑1 (MQ=60)
                             TTTTGAGGTTGGCTAATGAAACGCTTTCCGCTATTTCTTTTATTCACCCTGCTCACGCTGTCC‑ACC‑GTTCCGGCCCAGGCCGATATTATCGACG                                                                   <  SRR4302545.8674183/94‑1 (MQ=60)
                             TTTTGAGGTTGGCTAATGAAACGCTTTCCGCTATTTCTTTTATTCACCCTGCTCACGCTGCCC‑ACC‑GTTCCGGCCCAGGCCGATATTATCGACG                                                                   <  SRR4302545.3753859/94‑1 (MQ=60)
                              TTTGATGTTGGCTAATGAAACGCTTTCCGCTATTTCTTTTATTCACCCTGCTCACGCTGTCC‑ACC‑GTTCCGGCCCAGGCCGATATTATCGACGA                                                                  <  SRR4302545.754308/94‑1 (MQ=60)
                               TTGAGGTTGGCTAATGAAACGCTTTCCGCTATTTCTTTTATTCACCCTGCTCACGCTGTCC‑ACC‑GTTCCGGCCCAGGCCGATATTATCGACGAC                                                                 <  SRR4302545.9937132/94‑1 (MQ=60)
                                TGAGGTTGGCTAATGAAACGCTTTCCGCTATTTCTTTTATTCACCCTGCTCACGCTGTCC‑ACC‑GTTCCGGCCCAGGCCGATATTATCGACGACA                                                                <  SRR4302545.895248/94‑1 (MQ=60)
                                TGAGGTTGGCTAATGAAACGCTTTCCGCTATTTCTTTTATTCACCCTGCTCACGCTGTCC‑ACC‑GTTCCGGCCCAGGCCGATATTATCGACGACA                                                                <  SRR4302545.1654361/94‑1 (MQ=60)
                                TGAGGTTGGCTAATGAAACGCTTTCCGCTATTTCTTTTATTCACCCTGCTCACGCTGTCC‑ACC‑GTTCCGGCCCAGGCCGATATTATCGACGACA                                                                <  SRR4302545.1808921/94‑1 (MQ=60)
                                TGAGGTTGGCTAATGAAACGCTTTCCGCTATTTCTTTTATTCACCCTGCTCACGCTGTCC‑ACC‑GTTCCGGCCCAGGCCGATATTATCGACGACA                                                                <  SRR4302545.1055125/94‑1 (MQ=60)
                                TGAGGTTGGCTAATGAAACGCTTTCCGCTATTTCTTTTATTCACCCTGCTCACGCTGTCC‑ACC‑GTTCCGGCCCAGGCCGATATTATCGACGACA                                                                >  SRR4302545.1066639/1‑94 (MQ=60)
                                 GAGGTTGGCTAATGAAACGCTTTCCGCTATTTCTTTTATTCCCCCTGCTCACGCTGTCC‑ACC‑GTTCCGGCCCAGGCCGATATTATCGACGACAC                                                               <  SRR4302545.3694494/94‑1 (MQ=60)
                                 GAGGTTGGCTAATGAAACGCTTTCCGCTATTTCTTTTATTCACCCTGCTCACGCTGTCC‑ACC‑GTTCCGGCCCAGGCCGATATTATCGACGACAC                                                               >  SRR4302545.9151030/1‑94 (MQ=60)
                                   GGTTGGCTAATGAAACGCTTTCCGCTATTTCTTTTATTCACCCTGCTCACGCTGTCC‑ACC‑GTTCCGGCCCAGGCCGATATTATCGACGACACCA                                                             >  SRR4302545.5941764/1‑94 (MQ=60)
                                    GTTGTCTAATGAAACGCTTTCCGCTATTTCTTTTATTCTCCCTGCTCACGCTGTCC‑ACC‑GTTCCGGCCCAGGCCGATATTATCGACGACACCAT                                                            <  SRR4302545.8992376/94‑1 (MQ=60)
                                    GTTGGCTAATGAATCGCTTTCCGCTATTTCTTTTATTCCCCCTGCTCGCGCTGGCC‑ACC‑GTTCCGGCCCAGGCCGATATTATCGACGACACCAT                                                            <  SRR4302545.2615082/94‑1 (MQ=60)
                                    GTTGGCTAATGAAACTCTTTCCGCTATTTCTTTTATTCACCCTGCTCACGCTGTCC‑ACC‑GTTCCGGCCCAGGCCGATATTATCGACGACACCGT                                                            <  SRR4302545.8120580/94‑1 (MQ=60)
                                    GTTGGCTAATGAAACGCTTTCCGCTATTTCTTTTATTCACCCTGCTCACGCTGTCC‑ACC‑GTTCCGGCCCAGGCCGATATTATCGACGACACCAT                                                            >  SRR4302545.4401112/1‑94 (MQ=60)
                                    GTTGGCTAATGAAACGCTTTCCGCTATTTCTTTTATTCACCCTGCTCACGCTGTCC‑ACC‑GTTCCGGCCCAGGCCGATATTATCGACGACACCAT                                                            <  SRR4302545.8869052/94‑1 (MQ=60)
                                    GTTGGCTAATGAAACGCTTTCCGCTATTTCTTTTATTCACCCTGCTCACGCTGTCC‑ACC‑GTTCCGGCCCAGGCCGATATTATCGACGACACCAT                                                            <  SRR4302545.7458969/94‑1 (MQ=60)
                                    GTTGGCTAATGAAACGCTTTCCGCTATTTCTTTTATTCACCCTGCTCACGCTGTCC‑ACC‑GTTCCGGCCCAGGCCGATATTATCGACGACACCAT                                                            <  SRR4302545.8038324/94‑1 (MQ=60)
                                     TTGGCTAATGAAACGCTTTCCGCTATTTCTTTTATTCACCCTGCTCACGCTGTCC‑ACC‑GTTCCGGCCCAGGCCGATATTATCGACGACACCATT                                                           <  SRR4302545.8428552/94‑1 (MQ=60)
                                     TTGGCTAATGAAACGCTTTCCGCTATTTCTTTTATTCACCCTGCTCACGCTGTCC‑ACC‑GTTCCGGCCCAGGCCGATATTATCGACGACACAATT                                                           >  SRR4302545.1680888/1‑94 (MQ=60)
                                      TGGTTAATGAAACGCTTTCCGCTATTTCTTTTATTCACCCTGCTCACGCTGTCC‑ACC‑GTTCCGGCCCAGGCCGATATTATCGACGTCACCATTG                                                          <  SRR4302545.5637037/94‑1 (MQ=60)
                                      TGGCTAATGAAACGCTTTCCGCTATTTCTTTTATTCACCCTGCTCACGCTGTCC‑ACC‑GTTCCGGCCCAGGCCGATATTATCGACGACACCATTG                                                          <  SRR4302545.5637040/94‑1 (MQ=60)
                                      TGGCTAATGAAACGCTTTCCGCTATTTCTTTTATTCACCCTGCTCACGCTGTCC‑ACC‑GTTCCGGCCCAGGCCGATATTATCGACGACACCATTG                                                          <  SRR4302545.10661521/94‑1 (MQ=60)
                                      TGGCTAATGAAACGCTTTCCGCTATTTCTTTTATTCACCCTGCTCACGCTGTCC‑ACC‑GTTCCGGCCCAGGCCGATATTATCGACGACACCATTG                                                          <  SRR4302545.8202358/94‑1 (MQ=60)
                                       GGCTAATGAAACGCTTTCCGCTATTTCTTTTATTCACCCTGCTCACGCTGTCC‑ACC‑GTTCCGGCCCAGGCCGATATTATCGACGACACCATTGG                                                         <  SRR4302545.6894331/94‑1 (MQ=60)
                                       GGCTAATGAAACGCTTTCCGCTATTTCTTTTATTCACCCTGCTCACGCTGTCC‑ACC‑GTTCCGGCCCAGGCCGATATTATCGACGACACCATTGG                                                         <  SRR4302545.8064022/94‑1 (MQ=60)
                                       GGCTAATGAAACGCTTTCCGCTATTTCTTTTATTCACCCTGCTCACGCTGTCC‑ACC‑GTTCCGGCCCAGGCCGATATTATCGACGACACCATTGG                                                         >  SRR4302545.10651176/1‑94 (MQ=60)
                                       GGCTAATGAAACGCTTTCCGCTATTTCTTTTATTCACCCTGCTCACGCTGTCC‑ACC‑GTTCCGGCCCAGGCCGATATTATCGACGACACCATTGG                                                         <  SRR4302545.10866919/94‑1 (MQ=60)
                                        GCTAATGAAACGCTTTCCGCTATTTCTTTTATTCACCCTGCTCACGCTGTCC‑ACC‑GTTCCGGCCCAGGCCGATATTATCGACGACACCATTGGC                                                        >  SRR4302545.7130867/1‑94 (MQ=60)
                                         CTAATGAAACGCTTTCCGCTATTTCTTTTATTCACCCTGCTCACGCTGTCC‑ACC‑GTTCCGGCCCAGGCCGATATTATCGACGACACCATTGGCA                                                       >  SRR4302545.1091682/1‑94 (MQ=60)
                                         CTAATGAAACGCTTTCCGCTATTTCTTTTATTCACCCTGCTCACGCTGTCC‑ACC‑GTTCCGGCCCAGGCCGATATTATCGACGACACCATTGGCA                                                       <  SRR4302545.5676939/94‑1 (MQ=60)
                                         CTAATGAAACGCTTTCCGCTATTTCTTTTATTCACCCTGCTCACGCTGTCC‑ACC‑GTTCCGGCCCAGGCCGATATTATCGACGACACCATTGGCA                                                       >  SRR4302545.9063507/1‑94 (MQ=60)
                                         CTAATAAATCGGTTTCCGCTATTTCTTTTATGCACCCTGCTCACGCTGTCC‑ACC‑GTTCCGGCCCAGGCCGATATTATCGACGACGCCATTGGCA                                                       <  SRR4302545.372569/94‑1 (MQ=60)
                                          TAATGAAACGCTTTCCGCTATTTCTTTTATTCACCCTGCTCACGCTGTCC‑ACC‑GTTCCGGCCCAGGCCGATATTATCGACGACACCATTGGCAA                                                      >  SRR4302545.1071163/1‑94 (MQ=60)
                                           AATGAAACGCTTTCCGCTATTTCTTTTATTCACCCTGCTCACGCTGTCC‑ACC‑GTTCCGGCCCAGGCCGATATTATCGACGACACCATTGGCAAT                                                     >  SRR4302545.4384590/1‑94 (MQ=60)
                                           AATGAAACGCTTTCCGCTATTTCTTTTATTCACCCTGCTCACGCTGTCC‑ACC‑GTTCCGGCCCAGGCCGATATTATCGACGACACCATTGGCAAT                                                     <  SRR4302545.6794801/94‑1 (MQ=60)
                                            ATGAAACGCTTTCCGCTATTTCTTTTATTCACCCTGCTCACGCTGTCC‑ACC‑GTTCCGGCCCAGGCCGATATTATCGACGACACCATTGGCAATA                                                    >  SRR4302545.120933/1‑94 (MQ=60)
                                             TGAAACGCTTTCCGCTATTTCTTTTATTCACCCTGCTCACGCTGTCC‑ACC‑GTTCCGGCCCAGGCCGATATTATCGACGACACCATTGGCAATAT                                                   <  SRR4302545.7061689/94‑1 (MQ=60)
                                              GAAACGCTTTCCGCTATTTCTTTTATTCACCCTGCTCACGCTGTCC‑ACC‑GTTCCGGCCCAGGCCGATATTATCGACGACACCATTGGCAATATT                                                  >  SRR4302545.1729074/1‑94 (MQ=60)
                                              GAAACGCTTTCCGCTATTTCTTTTATTCACCCTGCTCACGCTGTCC‑ACC‑GTTCCGGCCCAGGCCGATATTATCGACGACACCATTGGCAATATT                                                  <  SRR4302545.9490300/94‑1 (MQ=60)
                                               AAACGCTTTCCGCTATTTCTTTTATTCACCCTGCTCACGCTGTCC‑ACC‑GTTCCGGCCCAGGCCGATATTATCGACGACACCATTGGCAATATTC                                                 >  SRR4302545.7646450/1‑94 (MQ=60)
                                               AAACGCTTTCCGCTATTTCTTTTATTCACCCTGCTAACGCTGTCC‑ACC‑GTTCCGGCCCAGGCCGATATTATCGACGACACCATTGGCACTATTC                                                 >  SRR4302545.586790/1‑94 (MQ=60)
                                                AACGCTTTCCTCTATTTCTTTTATTCACCCTGCTCACGTTGTCC‑ACC‑GTTCCGGCCCAGGCCGATATTATCGACGACACCATTGGCAATATTCA                                                <  SRR4302545.5705384/94‑1 (MQ=60)
                                                AACGCTTTCCGCTATTTCTTTTATTCACCCTGCTCACGCTGTCC‑ACC‑GTTCCGGCCCAGGCCGATATTATCGACGACACCATTGGCAATATTCA                                                >  SRR4302545.4684756/1‑94 (MQ=60)
                                                 ACGCTTTCCGCTATTTCTTTTATTCACCCTGCTCACGCTGTCC‑ACC‑GTTCCGGCCCAGGCCGATATTATCGACGACACCATTGGCAATATTCAG                                               <  SRR4302545.2130773/94‑1 (MQ=60)
                                                 ACGCTTTCCGCTATTTCTTTTATTCACCCTGCTCACGCTGTCC‑ACC‑GTTCCGGCCCAGGCCGATATTATCGACGACACCATTGGCAATATTCAG                                               <  SRR4302545.2156993/94‑1 (MQ=60)
                                                 ACGCTTTCCGCTATTTCTTTTATTCACCCTGCTCACGCTGTCC‑ACC‑GTTCCGGCCCAGGCCGATATTATCGACGACACCATTGGCAATATTCAG                                               <  SRR4302545.9548342/94‑1 (MQ=60)
                                                 ACGCTTTCCGCTATTTCTTTTATTCACCCTGCTCACGCTGTCC‑ACC‑GTTCCGGCCCAGGCCGATATTATCGACGACACCAATGGCAATATTCAG                                               <  SRR4302545.8709570/94‑1 (MQ=60)
                                                   GCTTTCCGCTATTTCTTTTATTCACCCTGCTCACGCTGTCC‑ACC‑GTTCCGGCCCAGGCCGATATTATCGACGACACCATTGGCAATATTCAGCA                                             >  SRR4302545.3213285/1‑94 (MQ=60)
                                                    CTTTCCGCTATTTCTTTTATTCACCCTGCTCACGCTGTCC‑ACC‑GTTCCGGCCCAGGCCGATATTATCGACGACACCATTGGCAATATTCAGCAG                                            <  SRR4302545.8969363/94‑1 (MQ=60)
                                                    CTTTCCGCTATTTCTTTTATTCACCCTGCTCACGCTGTCC‑ACC‑GTTCCGGCCCAGGCCGATATTATCGACGACACCATTGGCAATATTCAGCAG                                            <  SRR4302545.7931940/94‑1 (MQ=60)
                                                    CTTTCCGCTATTTCTTTTATTCACCCTGCTCACGCTGTCC‑ACC‑GTTCCGGCCCAGGCCGATATTATCGACGACACCATTGGCAATATTCAGCAG                                            >  SRR4302545.10700648/1‑94 (MQ=60)
                                                    CTTTCCGCTATTTCTTTTATTCACCCTGCTCACGCTGTCC‑ACC‑GTTCCGGCCCAGGCCGATATTATCGACGACACCATTGGCAATATTCAGCAG                                            >  SRR4302545.4637313/1‑94 (MQ=60)
                                                    CTTTCCGCTATTTCTTTTATTCACCCTGCTCACGCTGTCC‑ACC‑GTTCCGGCCCAGGCCGATATTATCGACGACACCATTGGCAATATTCAGCAG                                            >  SRR4302545.4764872/1‑94 (MQ=60)
                                                    CTTTCCGCTATTTCTTGTATTCACCCTGCTCACGCTGTCC‑ACC‑GTTCCGGCCAAGGCCGATATTATCGACGACCCCATTGGCAATATTCAGCAG                                            <  SRR4302545.6655954/94‑1 (MQ=60)
                                                     TTTCCGCTATTTCTTTTATTCACCCTGCTCACGCTGTCC‑ACC‑GTTCCGGCCCAGGCCGATATTATCGACGACACCATTGGCAATATTCAGCAGG                                           <  SRR4302545.3706899/94‑1 (MQ=60)
                                                      TCCCGCTATTTCTTTTATTCACCCTGCTCACGCTGTCC‑A‑CGGTTCCGGCCCAGGCCGATATTATCGACGACACCATTGGCAATATTCAGCAGGC                                          <  SRR4302545.4320609/94‑1 (MQ=60)
                                                       TCCTCTATTTCTTTTATTCCCCCTGCTCACGCTGGCC‑ACC‑GTTCCGGCCCAGGCCGATATTATCGACGACACCATTGGCAATATTCAGCAGGCG                                         <  SRR4302545.8370699/94‑1 (MQ=60)
                                                       TCCGCTATTTCTTTTATTCACCCTGCTCACGCTGTCC‑ACC‑GTTCCGGCCCAGGCCGATATTATCGACGACACCATTGGCAATATTCAGCAGGCG                                         >  SRR4302545.762598/1‑94 (MQ=60)
                                                        CCGCTATTTCTTTTATTCCCCCTGCTCACGCTGTCC‑ACC‑GTTCCGGCCCAGGCCGATATTATCGACGACACCATTGGCAATATTCAGCAGGCGA                                        <  SRR4302545.2942647/94‑1 (MQ=60)
                                                         CGCTATTTCTTTTATTCACCCTGCTCACGCTGTCC‑ACC‑GTTCCGGCCCAGGCCGATATTATCGACGACACCATTGGCAATATTCAGCAGGCGAT                                       >  SRR4302545.6670646/1‑94 (MQ=60)
                                                         CGCTATTTCTTTTATTCACCCTGCTCACGCTGTCC‑ACC‑GTTCCGGCCCAGGCCGATATTATCGACGACACCATTGGCAATATTCAGCAGGCGAT                                       >  SRR4302545.9830761/1‑94 (MQ=60)
                                                         CGCTATTTCTTTTATTCACCCTGCTCACGCTGTCC‑ACC‑GTTCCGGCCCAGGCCGATATTATCGACGACACCATTGGCAATATTCAGCAGGCGAT                                       >  SRR4302545.10011451/1‑94 (MQ=60)
                                                         CGCTATTTCTTTTATTCACCCTGCTCACGCTGTCC‑ACC‑GTTCCGGCCCAGGCCGATATTATCGACGACACCATTGGCAATATTCAGCAGGCGAT                                       >  SRR4302545.9449611/1‑94 (MQ=60)
                                                         CGCTATTTCTTTTATTCACCCTGCTCACGCTGTCC‑ACC‑GTTCCGGCCCAGGCCGATATTATCGACGACACCATTGGCAATATTCAGCAGGCGAT                                       >  SRR4302545.9478742/1‑94 (MQ=60)
                                                          GCTATTTCTTTTATTCACCCTGCTCACGCTGTCC‑ACC‑GTTCCGGCCCAGGCCGATATTATCGACGACACCATTGGCAATATTCAGCAGGCGATT                                      <  SRR4302545.9350744/94‑1 (MQ=60)
                                                          GCTATTTCTTTTATTCACCCTGCTCACGCTGTCC‑ACC‑GTTCCGGCCCAGGCCGATATTATCGACGACACCATTGGCAATATTCAGCAGGCGATT                                      >  SRR4302545.2889743/1‑94 (MQ=60)
                                                          GCTATTTCTTTTATTCACCCTGCTCACGCTGTCC‑ACC‑GTTCCGGCCCAGGCCGATATTATCGACGACACCATTGGCAATATTCAGCAGGCGATT                                      >  SRR4302545.5233462/1‑94 (MQ=60)
                                                          CCTATTTCTTTTATTCACCCTGCTCACGCTGTCC‑ACC‑GTTCCGGCCCAGGCCGATATTATCGACGACACCATTGGCAATATTCAGCAGGCGATT                                      <  SRR4302545.2804744/94‑1 (MQ=60)
                                                           CTATTTCTTTTATTCACCCTGCTCACGCTGTCC‑ACC‑GTTCCGGCCCAGGCCGATATTATCGACGACACCATTGGCAATATTCAGCAGGCGATTA                                     >  SRR4302545.3956313/1‑94 (MQ=60)
                                                            TATTTCTTTTATTCACCCTGCTCACGCTGTCC‑ACC‑GTTCCGGCCCAGGCCGATATTATCGACGACACCATTGGCAATATTCAGCAGGCGATTAA                                    >  SRR4302545.9918927/1‑94 (MQ=60)
                                                            TATTTCTTTTATTCACCCTGCTCACGCTGTCC‑ACC‑GTTCCGGCCCAGGCCGATATTATCGACGACACCATTGGCAATATTCAGCAGGCGATTAA                                    >  SRR4302545.7281758/1‑94 (MQ=60)
                                                            TATTTCTTTTATTCACCCTGCTCACGCTGTCC‑ACC‑GTTCCGGCCCAGGCCGATATTATCGACGACACCATTGGCAATATTCAGCAGGCGATTAA                                    <  SRR4302545.5659609/94‑1 (MQ=60)
                                                            TATTTCTTTTATTCACCCTGCTCACGCTGTCC‑ACC‑GTTCCGGCCCAGGCCGATATTATCCACGACACCATTGGCAATATTCAGCAGGCGATTAA                                    >  SRR4302545.1439936/1‑94 (MQ=60)
                                                            TATTTCTTTTATTCACCCTGATCACGCTGTCC‑ACC‑GTTCCGGACCAGGCCGATATTATCGACGACACCATTGGCAATATTCAGCAGGCGATTAA                                    >  SRR4302545.9918928/1‑94 (MQ=60)
                                                               TTCTTTTATTCACCCTGCTCACGCTGTCC‑ACC‑GTTCCGGCCCAGGCCGATATTATCGACGACACCATTGGCAATATTCAGCAGGCGATTAATGA                                 >  SRR4302545.6820016/1‑94 (MQ=60)
                                                               TTCTTTTATTCACCCTGCTCACGCTGTCC‑ACC‑GTTCCGGCCCAGGCCGATATTATCGACGACACCATTGGCAATATTCAGCAGGCGATTAATGA                                 >  SRR4302545.6067557/1‑94 (MQ=60)
                                                                TCTTTTATTCACCCTGCTCACGCTGTCC‑ACC‑GTTCCGGCCCAGGCCGATATTATCGACGACACCATTGGCAATATTCAGCAGGCGATTAATGAC                                >  SRR4302545.3541522/1‑94 (MQ=60)
                                                                TCTTTTATTCACCCTGCTCACGCTGTCC‑ACC‑GTTCCGGCCCAGGCCGATATTATCGACGACACCATTGGCAATATTCAGCAGGCGATTAATGAC                                >  SRR4302545.10368131/1‑94 (MQ=60)
                                                                TCTTTTATTCACCCTGCTCACGCTGTCC‑ACC‑GTTCCGGCCCAGGCCGATATTATCGACGACACCATTGGCAATATTCAGCAGGCGATTAATGAC                                <  SRR4302545.4865290/94‑1 (MQ=60)
                                                                TCTTTTATTCACCCTGCTCACGCTGTCC‑ACC‑GTTCCGCCCCAGGCCGAAATTATCGACGACACCATTGGCAATATTCAGCAGGCGATTAATGAC                                >  SRR4302545.2453528/1‑94 (MQ=60)
                                                                 TTTTTATTCACCCTGCTCACGCTGTCC‑ACC‑GTTCCGGCCCAGGCCGATATTATCGACGACACCATTGGCAATATTCAGCAGGCGATTAATGACG                               <  SRR4302545.3458776/94‑1 (MQ=60)
                                                                 CTTTTATTCACCCTGCTCACGCTGTCC‑ACC‑GTTCCGGCCCAGGCCGATATTATCGACGACACCATTGGCAATATTCAGCAGGCGATTAATGACG                               >  SRR4302545.5319946/1‑94 (MQ=60)
                                                                 CTTTTATTCACCCTGCTCACGCTGTCC‑ACC‑GTTCCGGCCCAGGCCGATATTATCGACGACACCATTGGCAATATTCAGCAGGCGATTAATGACG                               <  SRR4302545.7564324/94‑1 (MQ=60)
                                                                   TTTATTCACCCTGCTCACGCTGTCC‑ACC‑GTTCCGGCCCAGGCCGATATTATCGACGACACCATTGGCAATATTCAGCAGGCGATTAATGACGCT                             >  SRR4302545.6933410/1‑94 (MQ=60)
                                                                   TTTATTCACCCTGCTCACGCTGTCC‑ACC‑GTTCCGGCCCAGGCCGATATTATCGACGACACCATTGGCAATATTCAGCAGGCGATTAATGACGCT                             <  SRR4302545.3838964/94‑1 (MQ=60)
                                                                    TTATTCACCCTGCTGACGCTGTCC‑ACC‑GTTCCGGCCCAGGCCGATATTATCGACGACACCATTGGCAATATTCAGCAGGCGATTAATGACGCTT                            >  SRR4302545.2873793/1‑94 (MQ=60)
                                                                    TTATTCACCCTGCTCACGCTGTCC‑ACC‑GTTCCGGCCCAGGCCGATATTATCGACGACACCATTGGCAATATTCAGCAGGCGATTAATGACGCTT                            <  SRR4302545.5523643/94‑1 (MQ=60)
                                                                    TTATTCACCCTGCTCACGCTGTCC‑ACC‑GTTCCGGCCCAGGCCGATATTATCGACGACACCATTGGCAATATTCAGCAGGCGATTAATGACGCTT                            >  SRR4302545.3508155/1‑94 (MQ=60)
                                                                     TATTCACCCTGCTCACGCTGTCC‑ACC‑GTTCCGGCCCAGGCCGATATTATCGACGACACCATTGGCAATATTCATCAGGCGATTAATGACGCTTA                           <  SRR4302545.5397851/94‑1 (MQ=60)
                                                                     TATTCACCCTGCTCACGCTGTCC‑ACC‑GTTCCGGCCCAGGCCGATATTATCGACGACACCATTGGCAATATTCAGCAGGCGATTAATGACGCTTA                           <  SRR4302545.4260375/94‑1 (MQ=60)
                                                                      ATTCACCCTGCTCACGCTGTCC‑ACC‑GTTCCGGCCCAGGCCGATATTATCGACGACACCATTGGCAATATTCAGCAGGCGATTAATGACGCTTAT                          >  SRR4302545.6941105/1‑94 (MQ=60)
                                                                      ATTCACCCTGCTCACGCTGTCC‑ACC‑GTTCCGGCCCAGGCCGATATTATCGACGACACCATTGGCAATATTCAGCAGGCGATTAATGACGCTTAT                          >  SRR4302545.1945797/1‑94 (MQ=60)
                                                                      ATTCACCCTGCTCACGCTGTCC‑ACC‑GTTCCGGCCCAGGCCGATATTATCGACGACACCATTGGCAATATTCAGCAGGCGATTAATGACGCTTAT                          <  SRR4302545.2067608/94‑1 (MQ=60)
                                                                       TTCACCCTGCTCACGCTGTCC‑ACC‑GTTCCGGCCCAGGCCGATATTATCGACGACACCATTGGCAATATTCAGCAGGCGATTAATGACGCTTATA                         <  SRR4302545.1372734/94‑1 (MQ=60)
                                                                       TTCACCCTGCTCACGCTGTCC‑ACC‑GTTCCGGCCCAGGCCGATATTATCGACGACACCATTGGCAATATTCAGCAGGCGATTAATGACGCTTATA                         >  SRR4302545.495624/1‑94 (MQ=60)
                                                                       TTCACCCTGCTCACGCTGTCC‑ACC‑GTTCCGGCCCAGGCCGATATTATCGACGACACCATTGGCAATATTCAGCAGGCGATTAATGACGCTTATA                         <  SRR4302545.3911851/94‑1 (MQ=60)
                                                                       TTCACCCTGCTCACGCTGTCC‑ACC‑GTTCCGGCCCAGGCCGATATTATCGACGACACCATTGGCAATATTCAGCAGGCGATTAATGACGCTTATA                         >  SRR4302545.1514321/1‑94 (MQ=60)
                                                                        tcaccatggcaACGCTGTCC‑ACC‑GTTCCGCCCCAGGCCGATATTATCGACGACCCAATTGGCAATATTCAGCAGGCGATTAATGACGCTTATAA                        <  SRR4302545.4905980/83‑1 (MQ=60)
                                                                        TCACCCTGCTCACGCTGTCC‑ACC‑GTTCCGGCCCAGGCCGATATTATCGACGACACCATTGGCAATATTCAGCAGGCGATTAATGACGCTTATAA                        >  SRR4302545.3347626/1‑94 (MQ=60)
                                                                         CACCCTTCTCACCCTTTCC‑ACC‑GTTCCGGCCCAGGCCGATATTATCGACGACACCATTGGCAATATTCAGCAGGCGATTAATGACGCTTATAAC                       <  SRR4302545.8376471/94‑1 (MQ=60)
                                                                          CCCCTGCTCACGCTGTCC‑ACC‑GTTCCGGCCCAGGCCGATATTATCGACGACACCATTGGCAATATTCAGCAGGCGATTAATGACGCTTATAACC                      <  SRR4302545.618408/94‑1 (MQ=60)
                                                                          ACCCTGCTCACGCTGTCC‑ACC‑GTTCCGGCCCAGGCCGATATTATCGACGACACCATTGGCAATATTCAGCAGGCGATTAATGACGCTTATAACC                      >  SRR4302545.6094278/1‑94 (MQ=60)
                                                                           CCCTGCTCACGCTGTCC‑ACC‑GTTCCGGCCCAGGCCGATATTATCGACGACACCATTGGCAATATTCAGCAGGCGATTAATGACGCTTATAACCC                     <  SRR4302545.4255263/94‑1 (MQ=60)
                                                                           CCCTGCTCACGCTGTCC‑ACC‑GTTCCGGCCCAGGCCGATATTATCGACGACACCATTGGCAATATTCAGCAGGCGATTAATGAAGCTTATAACCC                     >  SRR4302545.3003319/1‑94 (MQ=60)
                                                                           CCCTGCTCACGCTGTCC‑ACC‑GTTCCGGCCCAGGCCGATATTATCGACGACACAATTGGCAATATTCAGCAGGCGATTAATGAACGTTATAACCC                     >  SRR4302545.5769421/1‑94 (MQ=60)
                                                                            CCTGCTCACGCTGTCC‑ACC‑GTTCCGGCCCAGGCCGATATTATCGACGACACCATTGGCAATATTCAGCAGGCGATTAATGACGCTTATAACCCT                    <  SRR4302545.3183602/94‑1 (MQ=60)
                                                                            CCTGCTCACGCTGTCC‑ACC‑GTTCCGGCCCAGGCCGATATTATCGACGACACCATTGGCAATATTCAGCAGGCGATTAATGACGCTTATAACCCT                    <  SRR4302545.6242134/94‑1 (MQ=60)
                                                                            CCTGCTCACGCTGTCC‑ACC‑GTTCCGGCCCAGGCCGATATTATCGACGACACCATTGGCAATATTCAGCAGGCGATTAATGACGCTTATAACCCT                    <  SRR4302545.2243116/94‑1 (MQ=60)
                                                                             CTGCTCACGCTGTCC‑ACC‑GTTCCGGCCCAGGCCGATATTATCGACGACACCATTGGCAATATTCAGCAGGCGATTAATGACGCTTATAACCCTG                   >  SRR4302545.4120607/1‑94 (MQ=60)
                                                                             CTGCTCACGCTGTCC‑ACC‑GTTCCGGCCCAGGCCGATATTATCGACGACACCATTGGCAATATTCAGCAGGCGATTAATGACGCTTATAACCCTG                   >  SRR4302545.3899883/1‑94 (MQ=60)
                                                                             CTGCTCACGCTGTCC‑ACC‑GTTCCGGCCCAGGCCGATATTATCGACGACACCATTGGCAATATTCAGCAGGCGATTAATGACGCTTATAACCCTG                   <  SRR4302545.7412804/94‑1 (MQ=60)
                                                                             CTGCTCACGCTGTCC‑ACC‑GTTCCGGCCCAGGCCGATATTATCGACGACACCATTGGCAATATTCAGCAGGCGATTAATGACGCTTATAACCCTG                   <  SRR4302545.2361611/94‑1 (MQ=60)
                                                                               GCTCACGCTGTCC‑ACC‑GTTCCGGCCCAGGCCGATATTATCGACGACACCATTGGCAATATTCAGCAGGCGATTAATGACGCTTATAACCCTGAC                 <  SRR4302545.7765408/94‑1 (MQ=60)
                                                                               GCTCACGCTGTCC‑ACC‑GTTCCGGCCCAGGCCGATATTATCGACGACACCATTGGCAATATTCAGCAGGCGATTAATGACGCTTATAACCCTGAC                 >  SRR4302545.6162934/1‑94 (MQ=60)
                                                                                CTCACGCTGTCC‑ACC‑GTTCCGGCCCAGGCCGATATTATCGACGACACCATTGGCAATATTCAGCAGGCGATTAATGACGCTTATAACCCTGACC                <  SRR4302545.10526055/94‑1 (MQ=60)
                                                                                CTCACGCTGTCC‑ACC‑GTTCCGGCCCAGGCCGATATTATCGACGACACCATTGGCAATATTCAGCAGGCGATTAATGACGCTTATAACCCTGACC                >  SRR4302545.6309960/1‑94 (MQ=60)
                                                                                  CACGCTGTCC‑ACC‑GTTCCGGCCCAGGCCGATATTATCGACGACACCATTGGCAATATTCAGCAGGCGATTAATGACGCTTATAACCCTGACCGT              >  SRR4302545.7718815/1‑94 (MQ=60)
                                                                                  CACGCTGTCC‑ACC‑GTTCCGGCCCAGGCCGATATTATCGACGACACCATTGGCAATATTCAGCAGGCGATTAATGACGCTTATAACCCTGACCGT              >  SRR4302545.6856223/1‑94 (MQ=60)
                                                                                   CCGCTGTCC‑ACC‑GTTCCGGCCCAGGCCGATATTATCGACGACACCATTGGCAATATTCAGCAGGCGATTAATGACGCTTATAACCCTGACCGTG             <  SRR4302545.1602282/94‑1 (MQ=60)
                                                                                   ACGCTGTCC‑ACC‑GTTCCGGCCCAGGCCGATATTATCGACGACACCATTGGCAATATTCATCAGGCGATTAATGACGCTTATAACCCTGACCGTG             >  SRR4302545.2857087/1‑94 (MQ=60)
                                                                                   ACGCTGTCC‑ACC‑GTTCCGGCCCAGGCCGATATTATCGACGACACCATTGGCAATATTCAGCAGGCGATTAATGACGCTTATAACCCTGACCGTG             <  SRR4302545.45050/94‑1 (MQ=60)
                                                                                   ACGCTGTCC‑ACC‑GTTCCGGCCCAGGCCGATATTATCGACGACACCATTGGCAATATTCAGCAGGCGATTAATGACGCTTATAACCCTGACCGTG             <  SRR4302545.646091/94‑1 (MQ=60)
                                                                                   ACGCTGTCC‑ACC‑GTTCCGGCCCAGGCCGATATTATCGACGACACCATTGGCAATATTCAGCAGGCGATTAATGACGCTTATAACCCTGACCGTG             >  SRR4302545.6589674/1‑94 (MQ=60)
                                                                                    CGCTGTCC‑ACC‑GTTCCGGCCCAGGCCGATATTATCGACGACACCATTGGCAATATTCAGCAGGCGATTAATGACGCTTATAACCCTGACCGTGG            <  SRR4302545.3282191/94‑1 (MQ=60)
                                                                                    CGCTGTCC‑ACC‑GTTCCGGCCCAGGCCGATATTATCGACGACACCATTGGCAATATTCAGCAGGCGATTAATGACGCTTATAACCCTGACCGTGG            >  SRR4302545.10422522/1‑94 (MQ=60)
                                                                                     GCTGTCC‑ACC‑GTTCCGGCCCAGGCCGATATTATCGACGACACCATTGGCAATATTCAGCAGGCGATTAATGACGCTTATAACCCTGACCGTGGG           >  SRR4302545.3961775/1‑94 (MQ=60)
                                                                                     GCTGTCC‑ACC‑GTTCCGGCCCAGGCCGATATTATCGACGACACCATTGGCAATATTCAGCAGGCGATTAATGACGCTTATAACCCTGACCGTGGG           <  SRR4302545.10519020/94‑1 (MQ=60)
                                                                                       TGTCC‑ACC‑GTTCCGGCCCAGGCCGATATTATCGACGACACCATTGGCAATATTCAGCAGGCGATTAATGACGCTTATAACCCTGACCGTGGGCG         >  SRR4302545.4305832/1‑94 (MQ=60)
                                                                                       TGTCC‑ACC‑GTTCCGGCCCAGGCCGATATTATCGACGACACCATTGGCAATATTCAGCAGGCGATTAATGACGCTTATAACCCTGACCGTGGGCG         >  SRR4302545.6845438/1‑94 (MQ=60)
                                                                                         TCC‑ACC‑GTTCCGGCCCAGGCCGATATTATCGACGACACCATTGGCAATATTCAGCAGGCGATTAATGACGCTTATAACCCTGACCGTGGGCGAG       >  SRR4302545.1964062/1‑94 (MQ=60)
                                                                                         TCC‑ACC‑GTTCCGGCCCAGGCCGATATTATCGACGACACCATTGGCAATATTCAGCAGGCGATTAATGACGCTTATAACCCTGACCGTGGGCGAG       >  SRR4302545.8815376/1‑94 (MQ=60)
                                                                                          tcctcC‑GTTCCGGCCCAGGCCGATATTATCGACGACACCATTGGCAATATTCAGCAGGCGATTAATGACGCTTATAACCCTGACCGTGGGCGAG       <  SRR4302545.4552313/89‑1 (MQ=60)
                                                                                           C‑C‑C‑GTTCCGGCCCAGGCCGATATTATCGACGACACCATTGGCAATATTCAGCAGGCGATTAATGACGCTTATAACCCTGACCGTGGGCGAGACT    <  SRR4302545.2154589/94‑1 (MQ=60)
                                                                                            cacC‑GTTCCGGCCCAGGCCGATATTATCGACGACACCATTGGCAATATTCAGCAGGCGATTAATGACGCTTATAACCCTGACCGTGGGCGAGAC     >  SRR4302545.8205675/4‑94 (MQ=60)
                                                                                            cacC‑GTTCCGGCCCAGGCCGATATTATCGACGACACCATTGGCAATATTCAGCAGGCGATTAATGACGCTTATAACCCTGACCGTGGGCGAGAC     >  SRR4302545.10024269/4‑94 (MQ=60)
                                                                                               C‑GTTCCGGCCCAGGCCGATATTATCGACGACACCATTGGCAATATTCAGCAGGCGATTAATGACGCTTATAACCCTGACCGTGGGCGAGACTAT  <  SRR4302545.5558907/94‑1 (MQ=60)
                                                                                               C‑GTTCCGGCCCAGGCCGATATTATCGACGACACCATTGGCAATATTCAGCAGGCGATTAATGACGCTTATAACCCTGACCGTGGGCGAGACTAT  <  SRR4302545.7059057/94‑1 (MQ=60)
                                                                                               C‑GTTCCGGCCCAGGCCGATATTATCGACGACACCATTGGCAATATTCAGCAGGCGATTAATGACGCTTATAACCCTGACCGTGGGCGAGACTAT  <  SRR4302545.10356892/94‑1 (MQ=60)
                                                                                               |                                                                                              
GAGTAATGATTAATGTGGATAGAGTTTCTTTTTGAGGTTGGCTAATGAAACGCTTTCCGCTATTTCTTTTATTCACCCTGCTCACGCTGTCC‑A‑C‑GTTCCGGCCCAGGCCGATATTATCGACGACACCATTGGCAATATTCAGCAGGCGATTAATGACGCTTATAACCCTGACCGTGGGCGAGACTAT  >  NC_000913/4311403‑4311589

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 2 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑