Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A1 F1 I1 R1	647	270.1	21258240	98.4%	20918108	94.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,474,826	+A	intergenic (+1/+44)	yjgL → / ← argI	hypothetical protein/ornithine carbamoyltransferase 1

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,474,823	1	.	A	99.8%	1546.1 / ‑4.2	396	coding (1849/1851 nt)	yjgL	hypothetical protein
Reads supporting (aligned to +/- strand): ref base . (1/0); new base A (261/134); total (262/134)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.95e-01

Only 100 of 429 total aligned reads displayed.

ATTAATGAACAGCGTTTAAATAAACTAAATCCACCGGAAAATTTACGTATAGCAATAGAACACTTTGGGTGGAAGAATAGACCTATCACTGCAT‑AAATAATGTGATGCCGGGATGGTTTCCGCATCTTATAGCGATCAGATTATTTACTGAGCGTCGCGACCATCACCGCTTTGATAGTATG  >  NC_000913/4474730‑4474911

 ttAATGAACAGCGTTTAAATAAACTAAATCCACCGGAAAATTTACGTATAGCAATAGAACACTTTGGGTGGAAGAATAGACCTATCACTGCat‑a                                                                                         <  1:3594378/94‑1 (MQ=255)

 ttAATGAACAGCGTTTAAATAAACTAAATCCACCGGAAAATTTACGTATAGCAATAGAACACTTTGGGTGGAAGAATAGACCTATCACTGCat‑a                                                                                         >  1:9947317/1‑94 (MQ=255)

  tAATGAACAGCGTTTAAATAAACTAAATCCACCGGAAAATTTACGTATAGCAATAGAACACTTTGGGTGGAAGAATAGACCTATCACTGCat‑aa                                                                                        >  2:9746206/1‑94 (MQ=255)

  tAATGAACAGCGTTTAAATAAACTAAATCCACCGGAAAATTTACGTATAGCAATAGAACACTTTGGGTGGAAGAATAGACCTATCACTGCat‑aa                                                                                        >  1:7358640/1‑94 (MQ=255)

   aaTGAACAGCGTTTAAATAAACTAAATCCACCGGAAAATTTACGTATAGCAATAGAACACTTTGGGTGGAAGAATAGACCTATCACTGCat‑aaa                                                                                       >  2:1339915/1‑94 (MQ=255)

     agAACAGCGTTTAAATAAACTAAATCCACCGGAAAATTTACGTATAGCAATAGAACACTTTGGGTGGAAGAATAGACCTATCACTGCat‑aaaat                                                                                     <  1:8646587/93‑3 (MQ=255)

       aaCAGCGTTTAAATAAACTAAATCCACCGGAAAATTTACGTATAGCAATAGAACACTTTGGGTGGAAGAATAGACCTATCACTGCat‑aaaataa                                                                                   >  1:5053251/1‑90 (MQ=255)

       aaCAGCGTTTAAATAAACTAAATCCACCGGAAAATTTACGTATAGCAATAGAACACTTTGGGTGGAAGAATAGACCTATCACTGCat‑aaaataa                                                                                   >  1:3570227/1‑90 (MQ=255)

       aaCAGCGTTTAAATAAACTAAATCCACCGGAAAATTTACGTATAGCAATAGAACACTTTGGGTGGAAGAATAGACCTATCACTGCat‑aaaataa                                                                                   <  2:282955/94‑5 (MQ=255)

       aaCAGCGTTTAAATAAACTAAATCCACCGGAAAATTTACGTATAGCAATAGAACACTTTGGGTGGAAGAATAGACCTATCACTGCat‑aaaataa                                                                                   >  2:2033893/1‑90 (MQ=255)

          aGCGTTTAAATAAACTAAATCCACCGGAAAATTTACGTATAGCAATAGAACACTTTGGGTGGAAGAATAGACCTATCACTGCATAAAATAAtgt                                                                                 >  1:655175/1‑94 (MQ=255)

           gCGTTTAAATAAACTAAATCCACCGGAAAATTTACGTATAGCAATAGAACACTTTGGGTGGAAGAATAGACCTATCACTGCATAAAATAAtgtg                                                                                <  2:1156264/94‑1 (MQ=255)

           gCGTTTAAATAAACTAAATCCACCGGAAAATTTACGTATAGCAATAGAACACTTTGGGTGGAAGAATAGACCTATCACTGCATAAAATAAtgtg                                                                                <  2:2637136/94‑1 (MQ=255)

             gTTTAAATAAACTAAATCCACCGGAAAATTTACGTATAGCAATAGAACACTTTGGGTGGAAGAATAGACCTATCACTGCATAAAATAATGTGAt                                                                              <  1:10100672/94‑1 (MQ=255)

              tttAAATAAACTAAATCCACCGGAAAATTTACGTATAGCAATAGAACACTTTGGGTGGAAGAATAGACCTATCACTGCATAAAATAATGTGATg                                                                             <  2:5224120/94‑1 (MQ=255)

                taaataaaCTAAATCCACCGGAAAATTTACGTATAGCAATAGAACACTTTGGGTGGAAGAATAGACCTATCACTGCATAAAATAATGTGATGcc                                                                           <  1:8988855/94‑1 (MQ=255)

                taaataaaCTAAATCCACCGGAAAATTTACGTATAGCAATAGAACACTTTGGGTGGAAGAATAGACCTATCACTGCATAAAATAATGTGATGcc                                                                           >  2:8498741/1‑94 (MQ=255)

                 aaataaaCTAAATCCACCGGAAAATTTACGTATAGCAATAGAACACTTTGGGTGGAAGAATAGACCTATCACTGCATAAAATAATGTGATGCCg                                                                          >  1:6349324/1‑94 (MQ=255)

                    taaaCTAAATCCACCGGAAAATTTACGTATAGCAATAGAACACTTTGGGTGGAAGAATAGACCTATCACTGCATAAAATAATGTGATGCCgggg                                                                       >  1:2725374/1‑93 (MQ=255)

                       aCTAAATCCACCGGAAAATTTACGTATAGCAATAGAACACTTTGGGTGGAAGAATAGACCTATCACTGCATAAAATAATGTGATGCCGGGATgg                                                                    >  1:5751496/1‑94 (MQ=255)

                          aaaTCCACCGGAAAATTTACGTATAGCAATAGAACACTTTGGGTGGAAGAATAGACCTATCACTGCATAAAATAATGTGATGCCGGGATGGttt                                                                 >  1:8862658/1‑94 (MQ=255)

                          aaaTCCACCGGAAAATTTACGTATAGCAATAGAACACTTTGGGCGGAAGAATAGACCTATCACTGCATAAAATAATGTGATGCCGGGATGGttt                                                                 >  1:6014373/1‑94 (MQ=255)

                             tCCACCGGAAAATTTACGTATAGCAATAGAACACTTTGGGTGGAAGAATAGACCTATCACTGCATAAAATAATGTGATGCCGGGATGGTTTgta                                                              >  2:5472744‑M1/1‑91 (MQ=255)

                              ccACCGGAAAATTTACGTATAGCAATAGAACACTTTGGGTGGAAGAATAGACCTATCACTGCATAAAATAATGTGATGCCGGGATGGTTTgtat                                                             >  1:5301993‑M1/1‑90 (MQ=255)

                              ccACCGGAAAATTTACGTATAGCAATAGAACACTTTGGGTGGAAGAATAGACCTATCACTGCATAAAATAATGTGATGCCGGGATGGTTTgtat                                                             <  1:688571‑M1/94‑5 (MQ=255)

                               cACCGGAAAATTTACGTATAGCAATAGAACACTTTGGGTGGAAGAATAGACCTATCACTGCATAAAATAATGTGATGCCGGGATGGTTTgtatt                                                            <  1:3065861‑M1/94‑6 (MQ=255)

                               cACCGGAAAATTTACGTATAGCAATAGAACACTTTGGGTGGAAGAATAGACCTATCACTGCATAAAATAATGTGATGCCGGGATGGTTTgtatt                                                            <  2:3746106‑M1/94‑6 (MQ=255)

                                aCCGGAAAATTTACGTATAGCAATAGAACACTTTGGGTGGAAGAATAGACCTATCACTGCATAAAATAATGTGATGCCGGGATGGTTTgtattt                                                           >  1:7223002‑M1/1‑88 (MQ=255)

                                aCCGGAAAATTTACGTATAGCAATAGAACACTTTGGGTGGAAGAATAGACCTATCACTGCATAAAATAATGTGATGCCGGGATGGTTTgtattt                                                           >  2:1010882‑M1/1‑88 (MQ=255)

                                 ccGGAAAATTTACGTATAGCAATAGAACACTTTGGGTGGAAGAATAGACCTATCACTGCATAAAATAATGTGATGCCGGGATGGTTTgtatttc                                                          >  1:2768130‑M1/1‑87 (MQ=255)

                                 ccGGAAAATTTACGTATAGCAATAGAACACTTTGGGTGGAAGAATAGACCTATCACTGCATAAAATAATGTGATGCCGGGATGGTTTgtatttc                                                          >  1:1070981‑M1/1‑87 (MQ=255)

                                  cGGAAAATTTACGTATAGCAATAGAACACTTTGGGTGGAAGAATAGACCTATCACTGCATAAAATAATGTGATGCCGGGATGGTTTgtatttcc                                                         >  1:5556876‑M1/1‑86 (MQ=255)

                                     aaaaTTTACGTATAGCAATAGAACACTTTGGGTGGAAGAATAGACCTATCACTGCATAAAATAATGTGATGCCGGGATGGTTTgtatttcccgg                                                      <  2:8625707‑M1/94‑12 (MQ=255)

                                       aaTTTACGTATAGCAATAGAACACTTTGGGTGGAAGAATAGACCTATCACTGCATAAAATAATGTGATGCCGGGATGGTTTgtatttcccggca                                                    <  2:4646871‑M1/94‑14 (MQ=255)

                                        aTTTACGTATAGCAATAGAACACTTTGGGTGGAAGAATAGACCTATCACTGCATAAAATAATGTGATGCCGGGATGGTTTgtatttcccggcat                                                   >  2:952526‑M1/1‑80 (MQ=255)

                                        aTTTACGTATAGCAATAGAACACTTTGGGTGGAAGAATAGACCTATCACTGCATAAAATAATGTGATGCCGGGATGGTTTgtatttcccggcat                                                   >  1:10595716‑M1/1‑80 (MQ=255)

                                          ttACGTATAGCAATAGAACACTTTGGGTGGAAGAATAGACCTATCACTGCATAAAATAATGTGATGCCGGGATGGTTTgtatttcccggcatct                                                 <  1:5411515‑M1/94‑17 (MQ=255)

                                          ttACGTATAGCAATAGAACACTTTGGGTGGAAGAATAGACCTATCACTGCATAAAATAATGTGATGCCGGGATGGTTTgtatttcccggcatct                                                 >  2:5136241‑M1/1‑78 (MQ=255)

                                           tACGTATAGCAATAGAACACTTTGGGTGGAAGAATAGACCTATCACTGCATAAAATAATGTGATGCCGGGATGGTTTgtatttcccggcatctt                                                <  2:9160438‑M1/94‑18 (MQ=255)

                                           tACGTATAGCAATAGAACACTTTGGGTGGAAGAATAGACCTATCACTGCATAAAATAATGTGATGCCGGGATGGTTTgtatttcccggcatctt                                                <  1:8337640‑M1/94‑18 (MQ=255)

                                             cGTATAGCAATAGAACACTTTGGGTGGAAGAATAGACCTATCACTGCATAAAATAATGTGATGCCGGGATGGTTTgtatttcccggcatcttta                                              >  2:4339760‑M1/1‑75 (MQ=255)

                                             cGTATAGCAATAGAACACTTTGGGTGGAAGAATAGACCTATCACTGCATAAAATAATGTGATGCCGGGATGGTTTgtatttcccggcatcttta                                              >  2:6011038‑M1/1‑75 (MQ=255)

                                             cGTATAGCAATAGAACACTTTGGGTGGAAGAATAGACCTATCACTGCATAAAATAATGTGATGCCGGGATGGTTTgtatttcccggcatcttta                                              <  1:9599722‑M1/94‑20 (MQ=255)

                                              gTATAGCAATAGAACACTTTGGGTGGAAGAATAGACCTATCACTGCATAAAATAATGTGATGCCGGGATGGTTTgtatttcccggcatctttat                                             >  1:3314082‑M1/1‑74 (MQ=255)

                                              gTATAGCAATAGAACACTTTGGGTGGAAGAATAGACCTATCACTGCATAAAATAATGTGATGCCGGGATGGTTTgtatttcccggaatctttat                                             <  1:1916987‑M1/94‑21 (MQ=255)

                                               tataGCAATAGAACACTTTGGGTGGAAGAATAGACCTATCACTGCATAAAATAATGTGATGCCGGGATGGTTTgtatttcccggcatctttata                                            >  1:9157559‑M1/1‑73 (MQ=255)

                                               tataGCAATAGAACACTTTGGGTGGAAGAATAGACCTATCACTGCATAAAATAATGTGATGCCGGGATGGTTTgtatttcccggcatctttata                                            <  2:5656315‑M1/94‑22 (MQ=255)

                                               tataGCAATAGAACACTTTGGGTGGAAGAATAGACCTATCACTGCATAAAATAATGTGATGCCGGGATGGTTTgtatttcccggcatctttata                                            <  2:4258192‑M1/94‑22 (MQ=255)

                                                ataGCAATAGAACACTTTGGGTGGAAGAATAGACCTATCACTGCATAAAATAATGTGATGCCGGGATGGTTTgtatttcccggcatctttatag                                           >  2:4696752‑M1/1‑72 (MQ=255)

                                                ataGCAATAGAACACTTTGGGTGGAAGAATAGACCTATCACTGCATAAAATAATGTGATGCCGGGATGGTTTgtatttcccggcatctttatag                                           >  2:8815243‑M1/1‑72 (MQ=255)

                                                 taGCAATAGAACACTTTGGGTGGAAGAATAGACCTATCACTGCATAAAATAATGTGATGCCGGGATGGTTTgtatttcccggcatctttatagc                                          >  1:4631956‑M1/1‑71 (MQ=255)

                                                 taGCAATAGAACACTTTGGGTGGAAGAATAGACCTATCACTGCATAAAATAATGTGATGCCGGGATGGTTTgtatttcccggcatctttatagc                                          <  2:342405‑M1/94‑24 (MQ=255)

                                                   gCAATAGAACACTTTGGGTGGAAGAATAGACCTATCACTGCATAAAATAATGTGATGCCGGGATGGTTTgtatttcccggcatctttatagcga                                        <  2:1567751‑M1/94‑26 (MQ=255)

                                                    cAATAGAACACTTTGGGTGGAAGAATAGACCTATCACTGCATAAAATAATGTGATGCCGGGATGGTTTgtatttcccggcatctttatagcgat                                       >  1:8216074‑M1/1‑68 (MQ=255)

                                                    cAATAGAACACTTTGGGTGGAAGAATAGACCTATCACTGCATAAAATAATGTGATGCCGGGATGGTTTgtatttcccggcatctttatagcgat                                       >  2:9941846‑M1/1‑68 (MQ=255)

                                                     aaTAGAACACTTTGGGTGGAAGAATAGACCTATCACTGCATAAAATAATGTGATGCCGGGATGGTTTgtatttcccggcatctttatagcgata                                      >  1:4860575‑M1/1‑67 (MQ=255)

                                                     aaTAGAACACTTTGGGTGGAAGAATAGACCTATCACTGCATAAAATAATGTGATGCCGGGATGGTTTgtatttcccggcatctttatagcgata                                      >  1:6079201‑M1/1‑67 (MQ=255)

                                                      aTAGAACACTTTGGGTGGAAGAATAGACCTATCACTGCATAAAATAATGTGATGCCGGGATGGTTTgtatttcccggcatctttatagcgatag                                     >  2:3192150‑M1/1‑66 (MQ=255)

                                                       tAGAACACTTTGGGTGGAAGAATAGACCTATCACTGCATAAAATAATGTGATGCCGGGATGGTTTgtatttcccggcatctttatagcgatagc                                    >  1:10001257‑M1/1‑65 (MQ=255)

                                                        aGAACACTTTGGGTGGAAGAATAGACCTATCACTGCATAAAATAATGTGATGCCGGGATGGTTTgtatttcccggcatctttatagcgatagca                                   >  2:5004010‑M1/1‑64 (MQ=255)

                                                        aGAACACTTTGGGTGGAAGAATAGACCTATCACTGCATAAAATAATGTGATGCCGGGATGGTTTgtatttcccggcatctttatagcgatagca                                   <  1:2477247‑M1/94‑31 (MQ=255)

                                                        aGAACACTTTGGGTGGAAGAATAGACCTATCACTGCATAAAATAATGTGATGCCGGGATGGTTTgtatttcccggcatctttatagcgatagca                                   >  2:7902326‑M1/1‑64 (MQ=255)

                                                         gAACACTTTGGGTGGAAGAATAGACCTATCACTGCATAAAATAATGTGATGCCGGGATGGTTTgtatttcccggcatctttatagcgatagcaa                                  <  2:7223942‑M1/94‑32 (MQ=255)

                                                         gAACACTATGGGTGGAAGAATAGACCTATCACTGCATAAAATAATGTGATGCCGGGATGGTTTgtatttcccggcatctttatagcnatagcaa                                  <  2:7152069‑M1/94‑32 (MQ=255)

                                                          aaCACTTTGGGTGGAAGAATAGACCTATCACTGCATAAAATAATGTGATGCCGGGATGGTTTgtatttcccggcatctttatagcgatagcaat                                 >  2:633096‑M1/1‑62 (MQ=255)

                                                          aaCACTTTGGGTGGAAGAATAGACCTATCACTGCATAAAATAATGTGATGCCGGGATGGTTTgtatttcccggcatctttatagcgatagcaat                                 <  1:7749457‑M1/94‑33 (MQ=255)

                                                           acacTTTGGGTGGAAGAATAGACCTATCACTGCATAAAATAATGTGATGCCGGGATGGTTTgtatttcccggcatctttatagcgatagcaatt                                <  1:6447443‑M1/94‑34 (MQ=255)

                                                              cTTTGGGTGGAAGAATAGACCTATCACTGCATAAAATAATGTGATGCCGGGATGGTTTgtatttcccggcatctttatagcgatagcaattatt                             >  2:5816349‑M1/1‑58 (MQ=255)

                                                              cTTTGGGTGGAAGAATAGACCTATCACTGCATAAAATAATGTGATGCCGGGATGGTTTgtatttcccggcatctttatagcgatagcaattatt                             <  2:1647247‑M1/94‑37 (MQ=255)

                                                                 tGGGTGGAAGAATAGACCTATCACTGCATAAAATAATGTGATGCCGGGATGGTTTgtatttcccggcatctttatagcgatagcaattatttac                          >  1:661190‑M1/1‑55 (MQ=255)

                                                                 tGGGTGGAAGAATAGACCTATCACTGCATAAAATAATGTGATGCCGGGATGGTTTgtatttcccggcatctttatagcgatagcaattatttac                          >  1:1405140‑M1/1‑55 (MQ=255)

                                                                    gTGGAAGAATAGACCTATCACTGCATAAAATAATGTGATGCCGGGATGGTTTgtatttcccggcatctttatagcgatagcaattatttactga                       >  2:748221‑M1/1‑52 (MQ=255)

                                                                     tGGAAGAATAGACCTATCACTGCATAAAATAATGTGATGCCGGGATGGTTTgtatttcccggcatctttatagcgatagcaattatttactgag                      <  2:10416561‑M1/94‑44 (MQ=255)

                                                                     tGGAAGAATAGACCTATCACTGCATAAAATAATGTGATGCCGGGATGGTTTgtatttcccggcatctttatagcgatagcaattatttactgag                      >  2:3129112‑M1/1‑51 (MQ=255)

                                                                      ggAAGAATAGACCTATCACTGCATAAAATAATGTGATGCCGGGATGGTTTgtatttcccggcatctttatagcgatagcaattatttactgagc                     >  1:7135397‑M1/1‑50 (MQ=255)

                                                                       gaagaaTAGACCTATCACTGCATAAAATAATGTGATGCCGGGATGGTTTgtatttcccggcatctttatagcgatagcaattatttactgagcg                    <  1:9763134‑M1/94‑46 (MQ=255)

                                                                        aagaaTAGACCTATCACTGCATAAAATAATGTGATGCCGGGATGGTTTgtatttcccggcatctttatagcgatagcaattatttactgagcgt                   >  2:458594‑M1/1‑48 (MQ=255)

                                                                        aagaaTAGACCTATCACTGCATAAAATAATGTGATGCCGGGATGGTTTgtatttcccggcatctttatagcgatagcaattatttactgagcgt                   >  1:5614717‑M1/1‑48 (MQ=255)

                                                                         agaaTAGACCTATCACTGCATAAAATAATGTGATGCCGGGATGGTTTgtatttcccggcatctttatagcgatagcaattatttactgagcgtc                  >  2:2248073‑M1/1‑47 (MQ=255)

                                                                             tAGACCTATCACTGCATAAAATAATGTGATGCCGGGATGGTTTgtatttcccggcatctttatagcgatagcaattatttactgagcgtcgcga              >  2:1265145‑M1/1‑43 (MQ=255)

                                                                              aGACCTATCACTGCATAAAATAATGTGATGCCGGGATGGTTTgtatttcccggcatctttatagcgatagcaattatttactgagcgtcgcgac             >  1:8180839‑M1/1‑42 (MQ=255)

                                                                              aGACCTATCACTGCATAAAATAATGTGATGCCGGGATGGTTTgtatttcccggcatctttatagcgatagcaattatttactgagcgtcgcgac             <  2:2579274‑M1/94‑53 (MQ=255)

                                                                              aGACCTATCACTGCATAAAATAATGTGATGCCGGGATGGTTTgtatttcccggcatctttatagcgatagcaattatttactgagcgtcgcgac             <  2:921988‑M1/94‑53 (MQ=255)

                                                                              aGACCTATCACTGCATAAAATAATGTGATGCCGGGATGGTTTgtatttcccggcatctttatagcgatagcaattatttactgagcgtcgcgac             >  2:3997244‑M1/1‑42 (MQ=255)

                                                                              aGACCTATCACTGCATAAAATAATGTGATGCCGGGATGGTTTgtatttcccggcatctttatagcgatagcaattatttactgagcgtcgcgac             >  2:5356772‑M1/1‑42 (MQ=255)

                                                                              aGACCTATCACTGCATAAAATAATGTGATGCCGGGATGGTTTgtatttcccggcatctttatagcgatagcaattatttactgagcgtcgcgac             >  2:574746‑M1/1‑42 (MQ=255)

                                                                                aCCTATCACTGCATAAAATAATGTGATGCCGGGATGGTTTgtatttcccggcatctttatagcgatagcaattatttactgagcgtcgcgacca           <  2:671128‑M1/94‑55 (MQ=255)

                                                                                 ccTATCACTGCATAAAATAATGTGATGCCGGGATGGTTTgtatttcccggcatctttatagcgatagcaattatttactgagcgtcgcgaccat          >  1:4028625‑M1/1‑39 (MQ=255)

                                                                                 ccTATCACTGCATAAAATAATGTGATGCCGGGATGGTTTgtatttcccggcatctttatagcgatagcaattatttactgagcgtcgcgaccat          <  1:10173518‑M1/94‑56 (MQ=255)

                                                                                  cTATCACTGCATAAAATAATGTGATGCCGGGATGGTTTgtatttcccggcatctttatagcgatagcaattatttactgagcgtcgcgaccatc         >  2:7588423‑M1/1‑38 (MQ=255)

                                                                                  cTATCACTGCATAAAATAATGTGATGCCGGGATGGTTTgtatttcccggcatctttatagcgatagcaattatttactgagcgtcgcgaccatc         <  1:7891199‑M1/94‑57 (MQ=255)

                                                                                  cTATCACTGCATAAAATAATGTGATGCCGGGATGGTTTgtatttcccggcatctttatagcgatagcaattatttactgagcgtcgcgaccatc         >  1:2059585‑M1/1‑38 (MQ=255)

                                                                                   tATCACTGCATAAAATAATGTGATGCCGGGATGGTTTgtatttcccggcatctttatagcgatagcaattatttactgagcgtcgcgaccatca        >  2:68628‑M1/1‑37 (MQ=255)

                                                                                    aTCACTGCATAAAATAATGTGATGCCGGGATGGTTTgtatttcccggcatctttatagcgatagcaattatttactgagcgtcgcgaccatcac       >  2:10280880‑M1/1‑36 (MQ=255)

                                                                                    aTCACTGCATAAAATAATGTGATGCCGGGATGGTTTgtatttcccggcatctttatagcgatagcaattatttactgagcgtcgcgaccatcac       >  1:4540277‑M1/1‑36 (MQ=255)

                                                                                     tCACTGCATAAAATAATGTGATGCCGGGATGGTTTgtatttcccggcatctttatagcgatagcaattatttactgagcgtcgcgaccatcacc      >  1:10403634‑M1/1‑35 (MQ=255)

                                                                                      cACTGCATAAAATAATGTGATGCCGGGATGGTTTgtatttcccggcatctttatagcgatagcaattatttactgagcgtcgcgaccatcaccg     >  1:1169795‑M1/1‑34 (MQ=255)

                                                                                      cACTGCATAAAATAATGTGATGCCGGGATGGTTTgtatttcccggcatctttatagcgatagcaattatttactgagcgtcgagaccatcaccg     >  1:2270069‑M1/1‑34 (MQ=255)

                                                                                        cTGCATAAAATAATGTGATGCCGGGATGGTTTgtatttcccggcatctttatagcgatagcaattatttactgagcgtcgcgaccatcaccgct   >  2:9042497‑M1/1‑32 (MQ=255)

                                                                                         tGCATAAAATAATGTGATGCCGGGATGGTTTgtatttcccggcatctttatagcgttagcaattattttttgagcgtggcgaccttcgccgctt  >  2:8087379‑M1/1‑31 (MQ=255)

ATTAATGAACAGCGTTTAAATAAACTAAATCCACCGGAAAATTTACGTATAGCAATAGAACACTTTGGGTGGAAGAATAGACCTATCACTGCAT‑AAATAATGTGATGCCGGGATGGTTTCCGCATCTTATAGCGATCAGATTATTTACTGAGCGTCGCGACCATCACCGCTTTGATAGTATG  >  NC_000913/4474730‑4474911

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 24 ≤` `ATCG/ATCG` `< 31 ≤` `ATCG/ATCG` `< 35 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A