Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I1 R1 647 270.1 21258240 98.4% 20918108 94.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 4,478,503 (A)7→6 intergenic (+12/+47) yjgN → / ← valS hypothetical protein/valine tRNA synthetase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,478,4970A.99.5% 1991.8 / 0.4 438intergenic (+6/+53)yjgN/valShypothetical protein/valine tRNA synthetase
Reads supporting (aligned to +/- strand):  ref base A (2/0);  new base . (223/212);  total (226/212)
Fisher's exact test for biased strand distribution p-value = 4.99e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 501 total aligned reads displayed.

TCCTTTAATCAATAAAGAAGAACAACCAGATAAAGGCTTCTTAGCCAGTATTTCACGGGGAGTTATGCCTTCTTTACCATTTCTGTAAGAGATAAAAAAAGGCCGGATGCATGCTCCGGCCTTGCGTTTTCATCACTGTGTTTTGATTACAGCGCGGCGATAACAGCCTGCTGTTCAATCAGTTT  >  NC_000913/4478404‑4478588
                                                                                             |                                                                                           
 ccttTAATCAATAAAGAAGAACAACCAGATAAAGGCTTCTTAGCCAGTATTTCACGGGGAGTTATGCCTTCTTTACCATTTCTGTAAGAGATaa                                                                                            <  1:5716390/94‑1 (MQ=255)
   tttAATCAATAAAGAAGAACAACCAGATAAAGGCTTCTTAGCCAGTATTTCACGGGGAGTTATGCCTTCTTTACCATTTCTGTAAGAGATaaaa                                                                                          >  2:7700253/1‑94 (MQ=255)
    ttAATCAATAAAGAAGAACAACCAGATAAAGGCTTCTTAGCCAGTATTTCACGGGGAGTTATGCCTTCTTTACCATTTCTGTAAGAGATaaaaa                                                                                         <  1:3862882/94‑1 (MQ=255)
       aTCAATAAAGAAGAACAACCAGATAAAGGCTTCTTAGCCAGTATTTCACGGGGAGTTATGCCTTCTTTACCATTTCTGTAAGAGATaaaaaagg                                                                                      <  2:9309748/94‑3 (MQ=255)
         cAATAAAGAAGAACAACCAGATAAAGGCTTCTTAGCCAGTATTTCACGGGGAGTTATGCCTTCTTTACCATTTCTGTAAGAGATaaaaaaggcc                                                                                    <  2:7831538/94‑5 (MQ=255)
           aTAAAGAAGAACAACCAGATAAAGGCTTCTTAGCCAGTATTTCACGGGGAGTTATGCCTTCTTTACCATTTCTGTAAGAGAT‑AAAAAAGGCCgg                                                                                 <  2:8403090/94‑1 (MQ=255)
           aTAAAGAAGAACAACCAGATAAAGGCTTCTTAGCCAGTATTTCACGGGGAGTTATGCCTTCTTTACCATTTCTGTAAGAGAT‑AAAAAAGGCCgg                                                                                 <  2:5026036/94‑1 (MQ=255)
            tAAAGAAGAACAACCAGATAAAGGCTTCTTAGCCAGTATTTCACGGGGAGTTATGCCTTCTTTACCATTTCTGTAAGAGAT‑AAAAAAGGCCGGa                                                                                >  1:10515429/1‑94 (MQ=255)
               agaagaACAACCAGATAAAGGGTTCTTAGCCAGTATTTCACGGGGAGTTATGCCTTCTTTACCATTTCTGTAAGAGAT‑AAAAAAGGCCGGagca                                                                             <  1:7058720/94‑4 (MQ=255)
               agaagaACAACCAGATAAAGGCTTCTTAGCCAGTATTTCACGGGGAGTTATGCCTTCTTTACCATTTCTGTAAGAGAT‑AAAAAAGGCCGGagca                                                                             <  2:4066502/94‑4 (MQ=255)
               agaagaACAACCAGATAAAGGCTTCTTAGCCAGTATTTCACGGGGAGTTATGCCTTCTTTACCATTTCTGTAAGAGAT‑AAAAAAGGCCGGagca                                                                             <  2:4355285/94‑4 (MQ=255)
               agaagaACAACCAGATAAAGGCTTCTTAGCCAGTATTTCACGGGGAGTTATGCCTTCTTTACCATTTCTGTAAGAGAT‑AAAAAAGGCCGGagca                                                                             <  1:655421/94‑4 (MQ=255)
                gaagaaCAACCAGATAAAGGCTTCTTAGCCAGTATTTCACGGGGAGTTATGCCTTCTTTACCATTTCTGTAAGAGAT‑AAAAAAGGCCGGagcat                                                                            <  2:633015/94‑5 (MQ=255)
                gaagaaCAACCAGATAAAGGCTTCTTAGCCAGTATTTCACGGGGAGTTATGCCTTCTTTACCATTTCTGTAAGAGAT‑AAAAAAGGCCGGagcat                                                                            <  1:9218353/94‑5 (MQ=255)
                gaagaaCAACCAGATAAAGGCTTCTTAGCCAGTATTTCACGGGGAGTTATGCCTTCTTTACCATTTCTGTAAGAGAT‑AAAAAAGGCCGGagcat                                                                            <  2:5334366/94‑5 (MQ=255)
                gaagaaCAACCAGATAAAGGCTTCTTAGCCAGTATTTCACGGGGAGTTATGCCTTCTTTACCATTTCTGTAAGAGAT‑AAAAAAGGCCGGagcat                                                                            <  1:8213480/94‑5 (MQ=255)
                   gaaCAACCAGATAAAGGCTTCTTAGCCAGTATTTCACGGGGAGTTATGCCTTCTTTACCATTTCTGTAAGAGAT‑AAAAAAGGCCGGA‑GCATGCt                                                                        <  1:9733037/94‑1 (MQ=255)
                     acaacCAGATAAAGGCTTCTTAGCCAGTATTTCACGGGGAGTTATGCCTTCTTTACCATTTCTGTAAGAGAT‑AAAAAAGGCCGGA‑GCATGCTcc                                                                      >  2:2295656/1‑94 (MQ=255)
                      caacCAGATAAAGGCTTCTTAGCCAGTATTTCACGGGGAGTTATGCCTTCTTTACCATTTCTGTAAGAGAT‑AAAAAAGGCCGGA‑GCATGCTCCg                                                                     >  2:1196428/1‑94 (MQ=255)
                       aacCAGATAAAGGCTTCTTAGCCAGTATTTCACGGGGAGTTATGCCTTCTTTACCATTTCTGTAAGAGAT‑AAAAAAGGCCGGA‑GCATGCTCCgg                                                                    <  1:6236567/94‑1 (MQ=255)
                       aacCAGATAAAGGCTTCTTAGCCAGTATTTCACGGGGAGTTATGCCTTCTTTACCATTTCTGTAAGAGAT‑AAAAAAGGCCGGA‑GCATGCTCCgg                                                                    <  2:10373270/94‑1 (MQ=255)
                       aacCAGATAAAGGCTTCTTAGCCAGTATTTCACGGGGAGTTATGCCTTCTTTACCATTTCTGTAAGAGAT‑AAAAAAGGCCGGA‑GCATGCTCCgg                                                                    >  1:7425376/1‑94 (MQ=255)
                        acCAGATAAAGGCTTCTTAGCCAGTATTTCACGGGGAGTTATGCCTTCTTTACCATTTCTGTAAGAGAT‑AAAAAAGGCCGGA‑GCATGCTCCGGc                                                                   >  2:9804624/1‑94 (MQ=255)
                        acCAGATAAAGGCTTCTTAGCCAGTATTTCACGGGGAGTTATGCCTTCTTTACCATTTCTGTAAGAGAT‑AAAAAAGGCCGGA‑GCATGCTCCGGc                                                                   <  2:1568503/94‑1 (MQ=255)
                          cAGATAAAGGCTTCTTAGCCAGTATTTCACGGGGAGTTATGCCTTCTTTACCATTTCTGTAAGAGAT‑AAAAAAGGCCGGA‑GCATGCTCCGGCCt                                                                 <  1:4481916/94‑1 (MQ=255)
                           aGATAAAGGCTTCTTAGCCAGTATTTCACGGGGAGTTATGCCTTCTTTACCATTTCTGTAAGAGAT‑AAAAAAGGCCGGA‑GCATGCTCCGGCCtt                                                                >  2:653611/1‑94 (MQ=255)
                            gATAAAGGCTTCTTAGCCAGTATTTCACGGGGAGTTATGCCTTCTTTACCATTTCTGTAAGAGAT‑AAAAAAGGCCGGA‑GCATGCTCCGGCCttc                                                               <  2:1762408/94‑2 (MQ=255)
                            gATAAAGGCTTCTTAGCCAGTATTTCACGGGGAGTTATGCCTTCTTTACCATTTCTGTAAGAGAT‑AAAAAAGGCCGGA‑GCATGCTCCGGCCttc                                                               <  1:3083896/94‑2 (MQ=255)
                            gATAAAGGCTTCTTAGCCAGTATTTCACGGGGAGTTATGCCTTCTTTACCATTTCTGTAAGAGAT‑AAAAAAGGACGGA‑GCATGCTCCGGCCttc                                                               >  1:5117401/1‑93 (MQ=255)
                             aTAAAGGCTTCTTAGCCAGTATTTCACGGGGAGTTATGCCTTCTTTACCATTTCTGTAAGAGAT‑AAAAAAGGCCGGA‑GCATGCTCCGGCCttcg                                                              >  2:4561345/1‑92 (MQ=255)
                             aTAAAGGCTTCTTAGCCAGTATTTCACGGGGAGTTATGCCTTCTTTACCATTTCTGTAAGAGAT‑AAAAAAGGCCGGA‑GCATGCTCCGGCCttcg                                                              >  2:7027322/1‑92 (MQ=255)
                             aTAAAGGCTTCTTAGCCAGTATTTCACGGGGAGTTATGCCTTCTTTACCATTTCTGTAAGAGAT‑AAAAAAGGCCCGA‑GCATGCTCCCGccctcg                                                              >  1:7530864/1‑90 (MQ=255)
                              tAAAGGCTTCTTAGCCAGTATTTCACGGGGAGTTATGCCTTCTTTACCATTTCTGTAAGAGAT‑AAAAAAGGCCGGA‑GCATGCTCCGGCCttcgt                                                             >  1:2752450/1‑91 (MQ=255)
                               aaaGGCTTCTTAGCCAGTATTTCACGGGGAGTTATGCCTTCTTTACCATTTCTGTAAGAGAT‑AAAAAAGGCCGGA‑GCATGCTCCGGCCttcgtt                                                            >  1:8553063/1‑90 (MQ=255)
                               aaaGGCTTCTTAGCCAGTATTTCACGGGGAGTTATGCCTTCTTTACCATTTCTGTAAGAGAT‑AAAAAAGGCCGGA‑GCATGCTCCGGCCttcgtt                                                            >  2:10166406/1‑90 (MQ=255)
                               aaaGGCTTCTTAGCCAGTATTTCACGGGGAGTTATGCCTTCTTTACCATTTCTGTAAGAGAT‑AAAAAAGGCCGGA‑GCATGCTCCGGCCttcgtt                                                            <  1:4652167/94‑5 (MQ=255)
                                aaGGCTTCTTAGCCAGTATTTCACGGGGAGTTATGCCTTCTTTACCATTTCTGTAAGAGAT‑AAAAAAGGCCGGA‑GCATGCTCCGGCCTT‑CGttt                                                          <  1:3743881/94‑1 (MQ=255)
                                 aGGCTTCTTAGCCAGTATTTCACGGGGAGTTATGCCTTCTTTACCATTTCTGTAAGAGAT‑AAAAAAGGCCGGA‑GCATGCTCCGGCCTT‑CGtttt                                                         >  2:6896902/1‑94 (MQ=255)
                                 aGGCTTCTTAGCCAGTATTTCACGGGGAGTTATGCCTTCTTTACCATTTCTGTAAGAGAT‑AAAAAAGGCCGGA‑GCATGCTCCGGCCTT‑CGtttt                                                         <  1:692467/94‑1 (MQ=255)
                                 aGGCTTCTTAGCCAGTATTTCACGGGGAGTTATGCCTTCTTTACCATTTCTGTAAGAGAT‑AAAAAAGGACGGA‑GCATGCACCGGCCTT‑CGtttt                                                         >  2:3903727/1‑94 (MQ=255)
                                  ggCTTCTTAGCCAGTATTTCACGGGGAGTTATGCCTTCTTTACCATTTCTGTAAGAGAT‑AAAAAAGGCCGGA‑GCATGCTCCGGCCTT‑CGTTTtc                                                        >  2:5528425/1‑94 (MQ=255)
                                  ggCTTCTTAGCCAGTATTTCACGGGGAGTTATGCCTTCTTTACCATTTCTGTAAGAGAT‑AAAAAAGGCCGGA‑GCATGCTCCCGCCTT‑CGTTTtc                                                        >  2:5837079/1‑94 (MQ=255)
                                  ggCTTCTTAGCCAGTATTTCACGGGGAGGTATGCCTTCTTTACCATTTCTGTAAGAGAT‑AAAAAAGGCCCGA‑GCATGCTCCCGCCCT‑CGTTTtc                                                        >  1:6414613/1‑94 (MQ=255)
                                   gCTTCTTAGCCAGTATTTCACGGGGAGTTATGCCTTCTTTACCATTTCTGTAAGAGAT‑AAAAAAGGCCGGA‑GCATGCTCCGGCCTT‑CGTTTtca                                                       >  2:10011455/1‑94 (MQ=255)
                                    cttcttAGCCAGTATTTCACGGGGAGTTATGCCTTCTTTACCATTTCTGTAAGAGAT‑AAAAAAGGCCGGA‑GCATGCTCCGGCCTT‑CGTTTtcat                                                      >  1:3509809/1‑94 (MQ=255)
                                    cttcttAGCCAGTATTTCACGGGGAGCTATGCCTTCTTTACCATTTCTGTAAGAGAT‑AAAAAAGGCCGGA‑GCATCCTCCGGCCGT‑CGTTTtctt                                                      >  2:8028363/1‑92 (MQ=255)
                                     ttcttAGCCAGTATTTCACGGGGAGTTATGCCTTCTTTACCATTTCTGTAAGAGAT‑AAAAAAGGCCGGA‑GCATGCTCCGGCCTT‑CGTTTtcatc                                                     >  2:3121836/1‑94 (MQ=255)
                                      tcttAGCCAGTATTTCACGGGGAGTTATGCCTTCTTTACCATTTCTGTAAGAGAT‑AAAAAAGGCCGGA‑GCATGCTCCGGCCTT‑CGTTTtcatca                                                    <  1:10067149/94‑1 (MQ=255)
                                       cttAGCCAGTATTTCACGGGGAGTTATGCCTTCTTTACCATTTCTGTAAGAGAT‑AAAAAAGGCCGGA‑GCATGCTCCGGCCTT‑CGTTTTCATCAc                                                   >  2:2207181/1‑94 (MQ=255)
                                         tAGCCAGTATTTCACGGGGAGTTATGCCTTCTTTACCATTTCTGTAAGAGAT‑AAAAAAGGCCGGA‑GCATGCTCCGGCCTT‑CGTTTTCATCACtg                                                 >  1:1619653/1‑94 (MQ=255)
                                          aGCCAGTATTTCACGGGGAGTTATGCCTTCTTTACCATTTCTGTAAGAGAT‑AAAAAAGGCCGGA‑GCATGCTCCGGCCTT‑CGTTTTCATCACtgt                                                >  2:6439738/1‑94 (MQ=255)
                                          aGCCAGTATTTCACGGGGAGTTATGCCTTCTTTACCATTTCTGTAAGAGAT‑AAAAAAGGCCGG‑CGCATGCTCCGGCCTT‑CGTTTTCATCACtgt                                                >  2:2030755/1‑94 (MQ=255)
                                             cAGTATTTCACGGGGAGTTATGCCTTCTTTACCATTTCTGTAAGAGAT‑AAAAAAGGCCGGA‑GCATGCTCCGGGCTT‑CGTTTTCATCACTGTGtt                                             >  1:878394/1‑94 (MQ=255)
                                             cAGTATTTCACGGGGAGTTATGCCTTCTTTACCATTTCTGTAAGAGAT‑AAAAAAGGCCGGA‑GCATGCTCCGGCCTT‑CGTTTTCATCACTGTGtt                                             <  2:9554931/94‑1 (MQ=255)
                                              aGTATTTCACGGGGAGTTATGCCTTCTTTACCATTTCTGTAAGAGAT‑AAAAAAGGCCGGA‑GCATGCTCCGGCCTT‑CGTTTTCATCACTGTGttt                                            >  1:6856556/1‑94 (MQ=255)
                                                tATTTCACGGGGAGTTATGCCTTCTTTACCATTTCTGTAAGAGAT‑AAAAAAGGCCGGA‑GCATGCTCCGGCCTT‑CGTTTTCATCACTGTGTTTTg                                          <  1:9874290/94‑1 (MQ=255)
                                                tATTTCACGGGGAGTTATGCCTTCTTTACCATTTCTGTAAGAGAT‑AAAAAAGGCCGGA‑GCATGCTCCGGCCTT‑CGTTTTCATCACTGTGTTTTg                                          <  1:7229116/94‑1 (MQ=255)
                                                tATTTCACGGGGAGTTATGCCTTCTTTACCATTTCTGTAAGAGAT‑AAAAAAGGCCGGA‑GCATGCTCCGGCCTT‑CGTTTTCATCACTGTGTTTTg                                          <  1:5380357/94‑1 (MQ=255)
                                                tATTTCACGGGGAGTTATGCCTTCTTTACCATTTCTGTAAGAGAT‑AAAAAAGGCCGGA‑GCATGCTCCGGCCTT‑CGTTTTCATCACTGTGTTTTg                                          <  2:898790/94‑1 (MQ=255)
                                                    tCACGGGGAGTTATGCCTTCTTTACCATTTCTGTAAGAGAT‑AAAAAAGGCCGGA‑GCATGCTCCGGCCTT‑CGTTTTCATCACTGTGTTTTGATTa                                      <  1:803617/94‑1 (MQ=255)
                                                       cGGGGAGTTATGCCTTCTTTACCATTTCTGTAAGAGAT‑AAAAAAGGCCGGA‑GCATGCTCCGGCCTT‑CGTTTTCATCACTGTGTTTTGATTACAg                                   >  2:6807536/1‑94 (MQ=255)
                                                       cGGGGAGTTATGCCTTCTTTACCATTTCTGTAAGAGAT‑AAAAAAGGCCGGA‑GCATGCTCCGGCCTT‑CGTTTTCATCACTGTGTTTTGATTACAg                                   <  1:7901431/94‑1 (MQ=255)
                                                         gggAGTTATGCCTTCTTTACCATTTCTGTAAGAGAT‑AAAAAAGGCCGGA‑GCACGCTCCGGCCTT‑CGTTTTCATCACTGTGTTTTGATTACAgcg                                 <  2:8622910/94‑1 (MQ=255)
                                                           gAGTTATGCCTTCTTTACCATTTCTGTAAGAGAT‑AAAAAAGGCCGGA‑GCATGCTCCGGCCTT‑CGTTTTCATCACTGTGTTTTGATTACAGcgcg                               <  1:4156233/94‑1 (MQ=255)
                                                             gTTATGCCTTCTTTACCATTTCTGTAAGAGAT‑AAAAAAGGCCGGA‑GCATGCTCCGGCCTT‑CGTTTTCATCACTGTGTTTTGATTACAGCgcggc                             <  1:1769728/94‑1 (MQ=255)
                                                                 tGCCTTCTTTACCATTTCTGTAAGAGAT‑AAAAAAGGCCGGA‑GCATGCTCCGGCCTT‑CGTTTTCATCACTGTGTTTTGATTACAGCGCGGCGATa                         <  2:8233208/94‑1 (MQ=255)
                                                                 tGCCTTCTTTACCATTTCTGTAAGAGAT‑AAAAAAGGCCGGA‑GCATGCTCCGGCCTT‑CGTTTTCATCACTGTGTTTTGATTACAGCGCGGCGATa                         <  2:8819841/94‑1 (MQ=255)
                                                                 tGCCTTCTTTACCATTTCTGTAAGAGAT‑AAAAAAGGCCGGA‑GCATGCTCCGGCCTT‑CGTTTTCATCACTGTGTTTTGATTACAGCGCGGCGATa                         <  2:7198568/94‑1 (MQ=255)
                                                                  gCCTTCTTTACCATTTCTGTAAGAGAT‑AAAAAAGGCCGGA‑GCATGCTCCGGCCTT‑CGTTTTCATCACTGTGTTTTGATTACAGCGCGGCGATaa                        <  1:1242043/94‑1 (MQ=255)
                                                                  gCCTTCTTTACCATTTCTGTAAGAGAT‑AAAAAAGGCCGGA‑GCATGCTCCGGCCTT‑CGTTTTCATCACTGTGTTTTGATTACAGCGCGGCGATaa                        >  1:9037518/1‑94 (MQ=255)
                                                                   ccTTCTTTACCATTTCTGTAAGAGAT‑AAAAAAGGCCGGA‑GCATGCTCCGGCCTT‑CGTTTTCATCACTGTGTTTTGATTACAGCGCGGCGATAAc                       >  1:10012170/1‑94 (MQ=255)
                                                                   ccTTCTTTACCATTTCTGTAAGAGAT‑AAAAAAGGCCGGA‑GCATGCTCCGGCCTT‑CGTTTTCATCACTGTGTTTTGATTACAGCGCGGCGATAAc                       >  1:2496890/1‑94 (MQ=255)
                                                                   ccTTCTTTACCATTTCTGTAAGAGAT‑AAAAAAGGCCGGA‑GCATGCTCCGGCCTT‑CGTTTTCATCACTGTGTTTTGATTACAGCGCGGCGATAAc                       >  2:4900657/1‑94 (MQ=255)
                                                                   ccTTCTTTACCATTTCTGTAAGAGAT‑AAAAAAGGCCGGA‑GCATGCTCCGGCCTT‑CGTTTTCATCACTGTGTTTTGATTACAGCGCGGCGATAAc                       >  2:2859163/1‑94 (MQ=255)
                                                                     ttcttTACCATTTCTGTAAGAGAT‑AAAAAAGGCCGGA‑GCATGCTCCGGCCTT‑CGTTTTCATCACTGTGTTTTGATTACAGCGCGGCGATAACAg                     >  2:3589468/1‑94 (MQ=255)
                                                                     ttcttTACCATTTCTGTAAGAGAT‑AAAAAAGGCCGGA‑GCATGCTCCGGCCTT‑CGTTTTCATCACTGTGTTTTGATTACAGCGCGGCGATAACAg                     <  1:5549761/94‑1 (MQ=255)
                                                                       cttTACCATTTCTGTAAGAGAT‑AAAAAAGGCCGGA‑GCATGCTCCGGCCTT‑CGTTTTCGTCACTGTGTTTTGATTACAGCGCGGCGATAACAGct                   >  2:4757417/1‑93 (MQ=255)
                                                                       cttTACCATTTCTGTAAGAGAT‑AAAAAAGGCCGGA‑GCATGCTCCGGCCTT‑CGTTTTCATCACTGTGTTTTGATTACAGCGCGGCGATAACAGcc                   >  1:2284893/1‑94 (MQ=255)
                                                                        tttACCATTTCTGTAAGAGAT‑AAAAAAGGCCGGA‑GCATGCTCCGGCCTT‑CGTTTTCATCACTGTGTTTTGATTACAGCGCGGCGATAACAGCct                  <  2:1846295/94‑1 (MQ=255)
                                                                        tttACCATTTCTGTAAGAGAT‑AAAAAAGGCCGGA‑GCATGCTCCGGCCTT‑CGTTTTCATCACTGTGTTTTGATTACAGCGCGGCGATAACAGCct                  <  2:6038015/94‑1 (MQ=255)
                                                                        tttACCATTTCTGTAAGAGAT‑AAAAAAGGCCGGA‑GCATGCTCCGGCCTT‑CGTTTTCATCACTGTGTTTTGATTACAGCGCGGCGATAACAGCct                  >  1:8876074/1‑94 (MQ=255)
                                                                         ttACCATTTCTGTAAGAGAT‑AAAAAAGGCCGGA‑GCATGCTCCGGCCTT‑CGTTTTCATCACTGTGTTTTGATTACAGCGCGGCGATAACAGCctg                 <  1:9420042/94‑1 (MQ=255)
                                                                         ttACCATTTCTGTAAGAGAT‑AAAAAAGGCCGGA‑GCATGCTCCGGCCTT‑CGTTTTCATCACTGTGTTTTGATTACAGCGCGGCGATAACAGCctg                 <  1:8349196/94‑1 (MQ=255)
                                                                          tACCATTTCTGTAAGAGAT‑AAAAAATGCCGGA‑GCATGCTCCGGCCTT‑CGTTTTCATCACTGTGTTTTGATTACAGCGCGGCGATAACAGCctgc                <  1:7769838/94‑1 (MQ=255)
                                                                          tACCATTTCTGTAAGAGAT‑AAAAAAGGCCGGA‑GCATGCTCCGGCCTT‑CGTTTTCATCACTGTGTTTTGATTACAGCGCGGCGATAACAGCctgc                >  2:3364981/1‑94 (MQ=255)
                                                                           aCCATTTCTGTAAGAGAT‑AAAAAAGGCCGGA‑GCATGCTCCGGCCTT‑CGTTTTCATCACTGTGTTTTGATTACAGCGCGGCGATAACAGCctgct               <  1:4987244/94‑1 (MQ=255)
                                                                                ttCTGTAAGAGA‑AAAAAAAGGCCGGA‑GCATGCTCCGGCCTT‑CGTTTTCATCACTGTGTTTTGATTACAGCGCGGCGATAACAGCCTGCTGTTCa          <  1:2043142/94‑1 (MQ=255)
                                                                                  cTGTAAGAGAT‑AAAAAAGGCCGGA‑GCATGCTCCGGCCTT‑CGTTTTCATCACTGTGTTTTGATTACAGCGCGGCGATAACAGCCTGCTGTTCAAt        >  2:5415149/1‑94 (MQ=255)
                                                                                   tGTAAGAGAT‑AAAAAAGGCCGGA‑GCATGCTCCGGCCTT‑CGTTTTCATCACTGTGTTTTGATTACAGCGCGGCGATAACAGCCTGCTGTTCAATc       <  2:9180724/94‑1 (MQ=255)
                                                                                   tGTAAGAGAT‑AAAAAAGGCCGGA‑GCATGCTCCGGCCTT‑CGTTTTCATCACTGTGTTTTGATTACAGCGCGGCGATAACAGCCCCCTGTTCAATc       >  1:10288037/1‑94 (MQ=255)
                                                                                    gTAAGAGAT‑AAAAAAGGCCGGA‑GCATGCTCCGGCCTT‑CGTTTTCATCACTGTGTTTTGATTACAGCGCGGCGATAACAGCCTGCTGTTCAATCa      >  1:3321972/1‑94 (MQ=255)
                                                                                    gTAAGAGAT‑AAAAAAGGCCGGA‑GCATGCTCCGGCCTT‑CGTTTTCATCACTGTGTTTTGATTACAGCGCGGCGATAACAGCCTGCTGTTCAATCa      <  1:8962495/94‑1 (MQ=255)
                                                                                    gTAAGAGAT‑AAAAAAGGCCGGA‑GCATGCTCCGGCCTT‑CGTTTTCATCACTGTGTTTTGATTACAGCGCGGCGATAACAGCCTGCTGTTCAATCa      <  2:3762021/94‑1 (MQ=255)
                                                                                     tAAGAGAT‑AAAAAATGCCGGA‑GCATGCTCCGGCCTT‑CGTTTTCATCACTGTGTTTTGATTACAGCGCGGCGATAACAGCCTGCTGTTCAATCAg     <  1:607126/94‑1 (MQ=255)
                                                                                      aaGAGAT‑AAAAAAGGCCGGA‑GCATGCTCCGGCCTT‑CGTTTTCATCACTGTGTTTTGATTACAGCGCGGCGATAACAGCCTGCTGTTCAATCAGt    >  2:7348215/1‑94 (MQ=255)
                                                                                      aaGAGAT‑AAAAAAGGCCGGA‑GCATGCTCCGGCCTT‑CGTTTTCATCACTGTGTTTTGATTACAGCGCGGCGATAACAGCCTGCTGTTCAATCAGt    >  2:5626119/1‑94 (MQ=255)
                                                                                      aaGAGAT‑AAAAAAGGCCGGA‑GCATGCTCCGGCCTT‑CGTTTTCATCACTGTGTTTTGATTACAGCGCGGCGATAACAGCCTGCTGTTCAATCAGt    <  2:2594111/94‑1 (MQ=255)
                                                                                      aaGAGAT‑AAAAAAGGCCGGA‑GCATGCTCCGGCCTT‑CGTTTTCATCACTGTGTTTTGATTACAGCGCGGCGATAACAGCCTGCTGTTCAATCAGt    <  2:9277203/94‑1 (MQ=255)
                                                                                       agagAT‑AAAAAAGGCCGGA‑GCATGCTCCGGCCTT‑CGTTTTCATCACTGTGTTTTGATTACAGCGCGGCGATAACAGCCTGCTGTTCAATCAGtt   <  2:9425232/94‑1 (MQ=255)
                                                                                        gagaT‑AAAAAAGGCCGGA‑GCATGCTCCGGCCTT‑CGTTTTCATCACTGTGTTTTGATTACAGCGCGGCGATAACAGCCTGCTGTTCAATCAGttt  <  1:2680538/94‑1 (MQ=255)
                                                                                             |                                                                                           
TCCTTTAATCAATAAAGAAGAACAACCAGATAAAGGCTTCTTAGCCAGTATTTCACGGGGAGTTATGCCTTCTTTACCATTTCTGTAAGAGATAAAAAAAGGCCGGATGCATGCTCCGGCCTTGCGTTTTCATCACTGTGTTTTGATTACAGCGCGGCGATAACAGCCTGCTGTTCAATCAGTTT  >  NC_000913/4478404‑4478588

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output Only 200 of 272 total aligned reads displayed.
AAAGAAGAACAACCAGATAAAGGCTTCTTAGCCAGTATTTCACGGGGAGTTATGCCTTCTTTACCATTTCTGTAAGAGATAAAAAAAGGCCGGATGCATGCTCCGGCCTTGCGTTTTCATCACTGTGTTTTGATTACAGCGCGGCGATAACAGCCTGCTGTTCAATCAGTTTCGCTTTCGCTTCCGCAT  >  NC_000913/4478417‑4478605
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AAAGAAGAACAACCAGATAAAGGCTTCTTAGCCAGTATTTCACGGGGAGTTATGCCTTCTTTACCATTTCTGTAAGAGAT‑AAAAAAGGCCGGAG                                                                                                >  SRR4302545.8169267/1‑94 (MQ=60)
AAAGAAGAACAACCAGATAAAGGCTTCTTAGCCAGTATTTCACGGGGAGTTATGCCTTCTTTACCATTTCTGTAAGAGA‑AAAAAAAGGCCGGAG                                                                                                >  SRR4302545.10314960/1‑94 (MQ=60)
  AGAAGAACAACCAGATAAAGGCTTCTTAGCCAGTATTTCACGGGGAGTTATGCCTTCTTTACCATTTCTGTAAGAGAT‑AAAAAAGGCCGGA‑GCA                                                                                             >  SRR4302545.10043764/1‑94 (MQ=60)
  AGAAGAACAACCAGATAAAGGCTTCTTAGCCAGTATTTCACGGGGAGTTATGCCTTCTTTACCATTTCTGTAAGAGAT‑AAAAAAGGCCGGA‑GCA                                                                                             <  SRR4302545.670031/94‑1 (MQ=60)
  AGAAGAACAACCAGATAAAGGCTTCTTAGCCAGTATTTCACGGGGAGTTATGCCTTCTTTACCATTTCTGTAAGAGAT‑AAAAAAGGCCGGA‑GCA                                                                                             <  SRR4302545.7253873/94‑1 (MQ=60)
  AGAAGAACAACCAGATAAAGGCTTCTTAGCCAGTATTTCACGGGGAGTTATGCCTTCTTTACCATTTCTGTAAGAGAT‑AAAAAAGGCCGGA‑GCA                                                                                             <  SRR4302545.7253875/94‑1 (MQ=60)
   GAAGAACAACCAGATAAAGGCTTCTTAGCCAGTATTTCACGGGGAGTTATGCCTTCTTTACCATTTCTGTAAGAGAT‑AAAAAAGGCCGGA‑GCAT                                                                                            <  SRR4302545.6461914/94‑1 (MQ=60)
   GAAGAACAACCAGATAAAGGCTTCTTAGCCAGTATTTCACGGGGAGTTATGCCTTCTTTACCATTTCTGTAAGAGAT‑AAAAAAGGCCGGA‑GCAT                                                                                            <  SRR4302545.6777957/94‑1 (MQ=60)
   GAAGAACAACCAGATAAAGGCTTCTTAGCCAGTATTTCACGGGGAGTTATGCCTTCTTTACCATTTCTGTAAGAGAT‑AAAAAAGGCCGGA‑GCAT                                                                                            <  SRR4302545.7088571/94‑1 (MQ=60)
   GAAGAACAACCAGATAAAGGCTTCTTAGCCAGTATTTCACGGGGAGTTATGCCTTCTTTACCATTTCTGTAAGAGAT‑AAAAAAGGCCGGA‑GCAT                                                                                            <  SRR4302545.8451253/94‑1 (MQ=60)
   GAAGAACAACCAGATAAAGGCTTCTTAGCCAGTATTTCACGGGGAGTTATGCCTTCTTTACCATTTCTGTAAGAGAT‑AAAAAAGGCCGGA‑GCAT                                                                                            <  SRR4302545.9488598/94‑1 (MQ=60)
   GAAGAACAACCAGATAAAGGCTTCTTAGCCAGTATTTCACGGGGAGTTATGCCTTCTTTACCATTTCTGTAAGAGAT‑AAAAAAGGCCGGA‑GCAT                                                                                            <  SRR4302545.6461912/94‑1 (MQ=60)
   GAAGAACAACCAGATAAAGGCTTATTAGCCAGTATTTCACGGGGAGTTATGCCTTCTTTACCATTTCTGTAAGAGAT‑AAAAAAGGCCGGA‑GCAT                                                                                            <  SRR4302545.7789790/94‑1 (MQ=60)
   AAAGAACAACCAGATAAAGGCTTCTTAGCCAGTATTTCACGGGGAGTTATGCCTTCTTTACCATTTCTGTAAGAGAT‑AAAAAAGGCCGGA‑GCAT                                                                                            <  SRR4302545.2192741/94‑1 (MQ=60)
   AAAGAACAACCAGATAAAGGCTTCTTAGCCAGTATTTCACGGGGAGTTATGCCTTCTTTACCATTTCTGTAAGAGAT‑AAAAAAGGCCGGA‑GCAT                                                                                            <  SRR4302545.3735643/94‑1 (MQ=60)
     AGAACAACCAGATAAAGGCTTCTTAGCCAGTATTTCACGGGGAGTTATGCCTTCTTTACCATTTCTGTAAGAGAT‑AAAAAAGGCCGGA‑GCATGC                                                                                          >  SRR4302545.462162/1‑94 (MQ=60)
     AGAACAACCAGATAAAGGCTTCTTAGCCAGTATTTCACGGGGAGTTATGCCTTCTTTACCATTTCTGTAAGAGAT‑AAAAAAGGCCGGA‑GCATGC                                                                                          >  SRR4302545.8108113/1‑94 (MQ=60)
     AGAACAACCAGATAAAGGCTTCTTAGCCAGTATTTCACGGGGAGTTATGCCTTCTTTACCATTTCTGTAAGAGAT‑AAAAAAGGCCGGA‑GCATGC                                                                                          >  SRR4302545.8108114/1‑94 (MQ=60)
      GAACAACCAGATAAAGGCTTCTTAGCCAGTATTTCACGGGGAGTTATGCCTTCTTTACCATTTCTGTAAGAGAT‑AAAAAAGGCCGGA‑GCATGCT                                                                                         <  SRR4302545.5875354/94‑1 (MQ=60)
      GAACAACCAGATAAAGGCTTCTTAGCCAGTATTTCACGGGGAGTTATGCCTTCTTTACCATTTCTGTAAGAGAT‑AAAAAAGGCCGGA‑GCATGCT                                                                                         <  SRR4302545.10022814/94‑1 (MQ=60)
       AACAACCAGATAAAGGCTTCTTAGCCAGTATTTCACGGGGAGTTATGCCTTCTTTACCATTTCTGTAAGAGAT‑AAAAAAGGCCGGA‑GGATGCTC                                                                                        >  SRR4302545.9507809/1‑94 (MQ=60)
       AACAACCAGATAAAGGCTTCTTAGCCAGTATTTCACGGGGAGTTATGCCTTCTTTACCATTTCTGTAAGAGAT‑AAAAAAGGCCGGA‑GCATGCTC                                                                                        >  SRR4302545.1982191/1‑94 (MQ=60)
        ACAACCAGATAAAGGCTTCTTAGCCAGTATTTCACGGGGAGTTATGCCTTCTTTACCATTTCTGTAAGAGAT‑AAAAAAGGCCGGA‑GCATGCTCC                                                                                       <  SRR4302545.9547694/94‑1 (MQ=60)
         cgacaAGATAAAGGCTTCTTAGCCAGTATTTCACGGGGAGTTATGCCTTCTTTGCCATTTCTGTAAGAGAT‑AAAAAAGGCCGGA‑GCATGCTCCG                                                                                      <  SRR4302545.10288686/89‑1 (MQ=60)
         CAACGAGATAAAGGCTTCTGAGCCAGTATTTCACGGGGAGTTATGCCTTCTTGACCATTTCTGTAAGAGAT‑AAAAAAGGCCGGA‑GCATGCTCCG                                                                                      <  SRR4302545.7703622/94‑1 (MQ=60)
         CAACCAGATAAAGGCTTCTTAGCCAGTATTTCACGGGGAGTTATGCCTTCTTTACCATTTCTGTAAGAGAT‑AAAAAAGGCCGGA‑GCATGCTCCG                                                                                      <  SRR4302545.1060306/94‑1 (MQ=60)
          AACCAGATAAAGGCTTCTTAGCCAGTATTTCACGGGGAGTTATGCCTTCTTTACCATTTCTGTAAGAGAT‑AAAAAAGGCCGGA‑GCATGCTCCGG                                                                                     >  SRR4302545.7635336/1‑94 (MQ=60)
          AACCAGATAAAGGCTTCTTAGCCAGTATTTCACGGGGAGTTATGCCTTCTTTACCATTTCTGTAAGAGAT‑AAAAAAGGCCGGA‑GCATGCTCCGG                                                                                     <  SRR4302545.698468/94‑1 (MQ=60)
          AACCAGATAAAGGCTTCTTAGCCAGTATTTCACGGGGAGTTATGCCTTCTTTACCATTTCTGTAAGAGAT‑AAAAAAGGCCGGA‑GCATGCTCCGG                                                                                     >  SRR4302545.8512853/1‑94 (MQ=60)
          AACCAGATAAAGGCTTCTTAGCCAGTATTTCACGGGGAGTTATGCCTTCTTTACCATTTCTGTAAGAGAT‑AAAAAAGGCCGGA‑GCATGCTCCGG                                                                                     <  SRR4302545.9257632/94‑1 (MQ=60)
          AACCAGATAAAGGCTTCTTAGCCAGTATTTCACGGGGAGTTATGCCTTCTTTACCATTTCTGTAAGAGAT‑AAAAAAGGCCGGA‑GCATGCTCCGG                                                                                     >  SRR4302545.8774294/1‑94 (MQ=60)
          AACCAGATAAAGGCTTCTTAGCCAGTATTTCACGGGGAGTTATGCCTTCTTTACCATTTCTGTAAGAGAT‑AAAAAAGGCCGGA‑GCATGCTCCGG                                                                                     <  SRR4302545.6406547/94‑1 (MQ=60)
           ACCAGATAAAGGCTTCTTAGCCAGTATTTCACGGGGAGTTATGCCTTCTTTACCATTTCTGTAAGAGAT‑AAAAAAGGCCGGA‑GCATGCTCCGGC                                                                                    >  SRR4302545.3911542/1‑94 (MQ=60)
           ACCAGATAAAGGCTTCTTAGCCAGTATTTCACGGGGAGTTATGCCTTCTTTACCATTTCTGTAAGAGAT‑AAAAAAGGCCGG‑GGCATGCTCCGGC                                                                                    >  SRR4302545.7858249/1‑94 (MQ=60)
            CCAGATAAAGGCTTCTTAGCCAGTATTTCACGGGGAGTTATGCCTTCTTTACCATTTCTGTAAGAGAT‑AAAAAAGGCCGGA‑GCATGCTCCGGCC                                                                                   >  SRR4302545.8258823/1‑94 (MQ=60)
            CCAGATAAAGGCTTCTTAGCCAGTATTTCACGGGGAGTTATGCCTTCTTTACCATTTCTGTAAGAGAT‑AAAAAAGGCCGGA‑GCATGCTCCGGCC                                                                                   >  SRR4302545.3266643/1‑94 (MQ=60)
            CCAGATAAAGGCTTCTTAGCCAGTATTTCACGGGGAGTTATGCCTTCTTTACCATTTCTGTAAGAGAT‑AAAAAAGGCCGGA‑GCATGCTCCGGCC                                                                                   >  SRR4302545.3427804/1‑94 (MQ=60)
            CCAGATAAAGGCTTCTTAGCCAGTATTTCACGGGGAGTTATGCCTTCTTTACCATTTCTGTAAGAGAT‑AAAAAAGGCCGGA‑GCATGCTCCGGCC                                                                                   >  SRR4302545.10618964/1‑94 (MQ=60)
             CAGATAAAGGCTTCTTAGCCAGTATTTCACGGGGAGTTATGCCTTCTTTACCATTTCTGTAAGAGAT‑AAAAAAGGCCGGA‑GCATGCTCCGGCCT                                                                                  <  SRR4302545.4596412/94‑1 (MQ=60)
             CAGATAAAGGCTTCTTAGCCAGTATTTCACGGGGAGTTATGCCTTCTTTACCATTTCTGTAAGAGAT‑AAAAAAGGCCGGA‑GCATGCTCCGGCCT                                                                                  >  SRR4302545.1683552/1‑94 (MQ=60)
             CAGATAAAGGCTTCTTAGCCAGTATTTCACGGGGAGTTATGCCTTCTTTACCATTTCTGTAAGAGAT‑AAAAAAGGCCGGA‑GCATGCTCCGGCCT                                                                                  <  SRR4302545.9098168/94‑1 (MQ=60)
             CAGATAAAGGCTTCTTAGCCAGTATTTCACGGGGAGTTATGCCTTCTTTACCATTTCTGTAAGAGAT‑AAAAAAGGCCGGA‑GCATGCTCCGGCCT                                                                                  <  SRR4302545.2604066/94‑1 (MQ=60)
              AGATAAAGGCTTCTTAGCCAGTATTTCACGGGGAGTTATGCCTTCTTTACCATTTCTGTAAGAGAT‑AAAAAAGGCCGGA‑GCATGCTCCGGCCTT                                                                                 <  SRR4302545.3674519/94‑1 (MQ=60)
              AGATAAAGGCTTCTTAGCCAGTATTTCACGGGGAGTTATGCCTTCTTTACCATTTCTGTAAGAGAT‑AAAAAAGGCCGGA‑GCATGCTCCGGCCTT                                                                                 <  SRR4302545.359271/94‑1 (MQ=60)
               GATAAAGGCTTCTTAGCCAGTATTTCACGGGGAGTTATGCCTTCTTTACCATTTCTGTAAGAGAT‑AAAAAAGGCCGGA‑GCATGCTCCGGCCTTC                                                                                <  SRR4302545.3159736/94‑1 (MQ=60)
               GATAAAGGCTTCTTAGCCAGTATTTCACGGGGAGTTATGCCTTCTTTACCATTTCTGTAAGAGAT‑AAAAAAGGACGGA‑GCATGCTCCGGCCTTC                                                                                >  SRR4302545.5248533/1‑94 (MQ=60)
                ATAAAGGCTTCTTAGCCAGTATTTCACGGGGAGTTATGCCTTCTTTACCATTTCTGTAAGAGAT‑AAAAAAGGCCGGA‑GCATGCTCCGGCCTTcg                                                                               >  SRR4302545.2805165/1‑92 (MQ=60)
                ATAAAGGCTTCTTAGCCAGTATTTCACGGGGAGTTATGCCTTCTTTACCATTTCTGTAAGAGAT‑AAAAAAGGCCGGA‑GCATGCTCCGGCCTTcg                                                                               >  SRR4302545.5494280/1‑92 (MQ=60)
                 TAAAGGCTTCTTAGCCAGTATTTCACGGGGAGTTATGCCTTCTTTACCATTTCTGTAAGAGAT‑AAAAAAGGCCGGA‑GCATGCTCCGGCCTT‑CGT                                                                             >  SRR4302545.2818855/1‑94 (MQ=60)
                  AAAGGCTTCTTAGCCAGTATTTCACGGGGAGTTATGCCTTCTTTACCATTTCTGTAAGAGAT‑AAAAAAGGCCGGA‑GCATGCTCCGGCCTT‑CGTT                                                                            >  SRR4302545.7067536/1‑94 (MQ=60)
                  AAAGGCTTCTTAGCCAGTATTTCACGGGGAGTTATGCCTTCTTTACCATTTCTGTAAGAGAT‑AAAAAAGGCCGGA‑GCATGCTCCGGCCTT‑CGTT                                                                            >  SRR4302545.9393001/1‑94 (MQ=60)
                  AAAGGCTTCTTAGCCAGTATTTCACGGGGAGTTATGCCTTCTTTACCATTTCTGTAAGAGAT‑AAAAAAGGCCGGA‑GCATGCTCCGGCCTT‑CGTT                                                                            <  SRR4302545.4770997/94‑1 (MQ=60)
                  AAAGGCTTCTTAGCCAGTATTTCACGGGGAGTTATGCCTTCTTTACCATTTCTGTAAGAGAT‑AAAAAAGGCCGGA‑GCATGCTCCGGCCTT‑CGTT                                                                            <  SRR4302545.9239539/94‑1 (MQ=60)
                  AAAGGCTTCTTAGCCAGTATTTCACGGGGAGTTATGCCTTCTTTACCATTTCTGTAAGAGAT‑AAAAAAGGCCGGA‑GCATGCTCCGGCCTT‑CGTT                                                                            >  SRR4302545.8800344/1‑94 (MQ=60)
                  AAAGGCTTCTTAGCAAGTATTTCACGGGGAGTTATGCCTTCTTTACCATTTCTGTAAGAGAT‑AAAAAAGGCCGGA‑GCATGCTCCGGCCTT‑CGTT                                                                            <  SRR4302545.2795387/94‑1 (MQ=60)
                   AAGGCTTCTTAGCCAGTATTTCACGGGGAGTTATGCCTTCTTTACCATTTCTGTAAGAGAT‑AACAAAGGCCGGA‑GCATCCTCCGGCCTT‑CGTTT                                                                           >  SRR4302545.8370453/1‑94 (MQ=60)
                   AAGGCTTCTTAGCCAGTATTTCACGGGGAGTTATGCCTTCTTTACCATTTCTGTAAGAGAT‑AAAAAAGGCCGGA‑GCATGCTCCGGCCTT‑CGTTT                                                                           >  SRR4302545.2794788/1‑94 (MQ=60)
                   AAGGCTTCTTAGCCAGTATTTCACGGGGAGTTATGCCTTCTTTACCATTTCTGTAAGAGAT‑AAAAAAGGCCGGA‑GCATGCTCCGGCCTT‑CGTTT                                                                           >  SRR4302545.8370452/1‑94 (MQ=60)
                     GGCTTCTTAGCCAGTATTTCACGGGGAGTTATGCCTTCTTTACCATTTCTGTAAGAGAT‑AAAAAAGGCCGGA‑GCATGCTCCGGCCTT‑CGTTTTC                                                                         <  SRR4302545.2456981/94‑1 (MQ=60)
                     GGCTTCTTAGCCAGTATTTCACGGGGAGTTATGCCTTCTTTACCATTTCTGTAAGAGAT‑AAAAAAGGCCGGA‑GCATGCTCCGGCCTT‑CGTTTTC                                                                         <  SRR4302545.7441021/94‑1 (MQ=60)
                     GGCTTCTTAGCCAGTATTTCACGGGGAGTTATGCCTTCTTTACCATTTCTGTAAGAGAT‑AAAAAAGGCCGGA‑GCATGCTCCGGCCTT‑CGTTTTC                                                                         <  SRR4302545.7981346/94‑1 (MQ=60)
                      GCTTCTTAGCCAGTATTTCACGGGGAGTTATGCCTTCTTTACCATTTCTGTAAGAGAT‑AAAAAAGGACGGA‑GCATGCTCCGGCCTT‑CGTTTTCA                                                                        >  SRR4302545.4319547/1‑94 (MQ=60)
                       CTTCTTAGCCAGTATTTCACGGGGAGTTATGCCTTCTTTACCATTTCTGTAAGAGAT‑AAAAAAGGCCGGA‑GCATGCTCCGGCCTT‑CGTTTTCAT                                                                       <  SRR4302545.25418/94‑1 (MQ=60)
                       CTTCTTAGCCAGTATTTCACGGGGAGTTATGCCTTCTTTACCATTTCTGTAAGAGAT‑AAAAAAGGCCGGA‑GCATGCTCCGGCCTT‑CGTTTTCAT                                                                       >  SRR4302545.3595892/1‑94 (MQ=60)
                         TCTTAGCCAGTATTTCACGGGGAGTTATGCCTTCTTTACCATTTCTGTAAGAGAT‑AAAAAAGGCCGGA‑GCATGCTCCGGCCTT‑CGTTTTCATCA                                                                     <  SRR4302545.4643785/94‑1 (MQ=60)
                          CTTAGCCAGTATTTCACGGGGAGTTATGCCTTCTTTACCATTTCTGTAAGAGAT‑AAAAAAGGCCGGA‑GCATGCTCCGGCCTT‑CGTTTTCATCAC                                                                    >  SRR4302545.9343466/1‑94 (MQ=60)
                          CTTAGCCAGTATTTCACGGGGAGTTATGCCTTCTTTACCATTTCTGTAAGAGAT‑AAAAAAGGCCGGA‑GCATGCTCCGGCCTT‑CGTTTTCATCAC                                                                    >  SRR4302545.10449810/1‑94 (MQ=60)
                            TAGCCAGTATTTCACGGGGAGTTATGCCTTCTTTTCCATTTCTGTAAGAGAT‑AAAAAAGGCCGGA‑GCATGCTCCGGCCTT‑CGTTTTCATCACTG                                                                  <  SRR4302545.2843874/94‑1 (MQ=60)
                             AGCCAGTATTTCACGGGGAGTTATGCCTTCTTTACCATTTCTGTAAGAGAT‑AAAAAAGGCCGGA‑GCAAGCTCCGGCCCT‑CGTTTTCATCACTGT                                                                 >  SRR4302545.6389329/1‑94 (MQ=60)
                              GCCAGTATTTCACGGGGAGTTATGCCTTCTTTACCATTTCTGTAAGAGAT‑AAAAAAGGCCGGA‑GCATGCTCCGGCCTT‑CGTTTTCATCACTGTG                                                                >  SRR4302545.9192080/1‑94 (MQ=60)
                               CCAGTATTTCACGGGGAGTTATGCCTTCTTTACCATTTCTGTAAGAGAT‑AAAAAAGGCCGGA‑GCATGCTCCGGCCTT‑CGTTTTCATCACTGTGT                                                               >  SRR4302545.5093614/1‑94 (MQ=60)
                               CCAGTATTTCACGGGGAGTTATGCCTTCTTTACCATTTCTGTAAGAGAT‑AAAAAAGGCCGGA‑GCATGCTCCGGCCTT‑CGTTTTCATCACTGTGT                                                               <  SRR4302545.1415562/94‑1 (MQ=60)
                               CCAGTATTTCACGGGGAGTTATGCCTTCTTTACCATTTCTGTAAGAGAT‑AAAAAAGGCCGGA‑GCATGCTCCGGCCTT‑CGTTTTCATCACTGTGT                                                               >  SRR4302545.6712345/1‑94 (MQ=60)
                                CAGTATTTCACGGGGAGTTATGCCTTCTTTACCATTTCTGTAAGAGAT‑AAAAAAGGCCGGA‑GCATGCTCCGGGCTT‑CGTTTTCATCACTGTGTT                                                              >  SRR4302545.898114/1‑94 (MQ=60)
                                CAGTATTTCACGGGGAGTTATGCCTTCTTTACCATTTCTGTAAGAGAT‑AAAAAAGGCCGGA‑GCATGCTCCGGCCTT‑CGTTTTCATCACTGTGTT                                                              >  SRR4302545.6139899/1‑94 (MQ=60)
                                 AGTATTTCACGGGGAGTTATGCCTTCTTTACCATTTCTGTAAGAGAT‑AAAAAAGGCCGGA‑GCATGCTCCGGCCTT‑CGTTTTCATCACTGTGTTT                                                             >  SRR4302545.7044915/1‑94 (MQ=60)
                                 AGTATTTCACGGGGAGTTATGCCTTCTTTACCATTTCTGTAAGAGAT‑AAAAAAGGCCGGA‑GCATGCTCCGGCCTT‑CGTTTTCATCACTGTGTTT                                                             >  SRR4302545.10107136/1‑94 (MQ=60)
                                 AGTATTTCACGGGGAGTTATGCCTTCTTTACCATTTCTGTAAGAGAT‑AAAAAAGGCCGGA‑GCATGCTCCGGCCTT‑CGTTTTCATCACTGTGTTT                                                             >  SRR4302545.7045205/1‑94 (MQ=60)
                                 AGTATTTCACGGGGAGTTATGCCTTCTTTACCATTTCTGTAAGAGAT‑AAAAAAGGCCGGA‑GCATGCTCCGGCCTT‑CGTTTTCATCACTGTGTTT                                                             <  SRR4302545.6614307/94‑1 (MQ=60)
                                 AGTATTTCACGGGGAGTTATGCCTTCTTTACCATTTCTGTAAGAGAT‑AAAAAAGGCCGGA‑GCATGCTCCGGCCTT‑CGTTTTCATCACTGTGTTT                                                             >  SRR4302545.8852786/1‑94 (MQ=60)
                                  GTATTTCACGGTGAGTTATGCCTTCTTTACCATTTCTGTAAGAGAT‑AAAAAAGGCCGGA‑GCATGCTCCGGCCTT‑CGTTTTCATCACTGTGTTTT                                                            <  SRR4302545.5555072/94‑1 (MQ=60)
                                  GTATTTCACGGGGAGTTATGCCTTCTTTACCATTTCTGTAAGAGAT‑AAAAAAGGCCGGA‑GCATGCTCCGGCCTT‑CGTTTTCATCACTGTGTTTT                                                            <  SRR4302545.9009980/94‑1 (MQ=60)
                                  GTATTTCACGGGGAGTTATGCCTTCTTTACCATTTCTGTAAGAGAT‑AAAAAAGGCCGGA‑GCATGCTCCGGCCTT‑CGTTTTCATCACTGTGTTTT                                                            >  SRR4302545.1684763/1‑94 (MQ=60)
                                  GTATTTCACGGGGAGTTATGCCTTCTTTACCATTTCTGTAAGAGAT‑AAAAAAGGCCGGA‑GCATGCTCCGGCCTT‑CGTTTTCATCACTGTGTTTT                                                            <  SRR4302545.6967731/94‑1 (MQ=60)
                                  GTATTTCACGGGGAGTTATGCCTTCTTTACCATTTCTGTAAGAGAT‑AAAAAAGGCCGGA‑GCATGCTCCGGCCTT‑CGTTTTCATCACTGTGTTTT                                                            <  SRR4302545.5555071/94‑1 (MQ=60)
                                  GTATTTCACGGGGAGTTATGCCTTCTTTACCATTTCTGTAAGAGAT‑AAAAAAGGCCGGA‑GCATGCTCCGGCCTT‑CGTTTTCATCACTGTGTTTT                                                            >  SRR4302545.3934174/1‑94 (MQ=60)
                                   TATTTCACGGGGAGTTATGCCTTCTTTACCATTTCTGTAAGAGAT‑AAAAAAGGCCGGA‑GCATGCTCCGGCCTT‑CGTTTTCATCACTGTGTTTTG                                                           <  SRR4302545.5520358/94‑1 (MQ=60)
                                   TATTTCACGGGGAGTTATGCCTTCTTTACCATTTCTGTAAGAGAT‑AAAAAAGGCCGGA‑GCATGCTCCGGCCTT‑CGTTTTCATCACTGTGTTTTG                                                           <  SRR4302545.5520357/94‑1 (MQ=60)
                                   TATTTCACGGGGAGTTATGCCTTCTTTACCATTTCTGTAAGAGAT‑AAAAAAGGCCGGA‑GCATGCTCCGGCCTT‑CGTTTTCATCACTGTGTTTTG                                                           <  SRR4302545.10167724/94‑1 (MQ=60)
                                   TATTTCACGGGGAGATATGCCTTCTTTACCATTTCTGTAAGAGAT‑AAAAAAGGCCGGA‑GCATGCTCCCGCCTT‑CGTTTTCATCACTGTGGTTTG                                                           >  SRR4302545.578384/1‑94 (MQ=60)
                                      TTCACGGGGAGTTATGCCTTCTTTACCATTTCTGTAAGAGAT‑AAAAAAGGCCGGA‑GCATGCTCCGGCCTT‑CGTTTTCATCACTGTGTTTTGATT                                                        <  SRR4302545.5614638/94‑1 (MQ=60)
                                       TCACGGGGAGTTATGCCTTCTTTACCATTTCTGTAAGAGAT‑AAAAAAGGCCGGA‑GCATGCTCCGGCCTT‑CGTTTTCATCACTGTGTTTTGATTA                                                       <  SRR4302545.7286924/94‑1 (MQ=60)
                                       TCACGGGGAGTTATGCCTTCTTTACCATTTCTGTAAGAGAT‑AAAAAAGGCCGGA‑GCATGCTCCGGCCTT‑CGTTTTCATCACTGTGTTTTGATTA                                                       <  SRR4302545.821659/94‑1 (MQ=60)
                                       TCACGGGGAGTTATGCCTTCTTTACCATTTCTGTAAGAGAT‑AAAAAAGGCCGGA‑GCATGCTCCGGCCTT‑CGTTTTCATCACTGTGTTTTGATTA                                                       <  SRR4302545.4359553/94‑1 (MQ=60)
                                         ACGGGGAGTTATGCCTTCTTTACCATTTCTGTAAGAGAT‑AAAAAAGGCCGGA‑GCATGCTCCGGCCTT‑CGTTTTCATCACTGTGTTTTGATTACA                                                     <  SRR4302545.10268416/94‑1 (MQ=60)
                                          CGGGGAGTTATGCCTTCTTTACCATTTCTGTAAGAGAT‑AAAAAAGGCCGGA‑GCATGCTCCGGCCTT‑CGTTTTCATCACTGTGTTTTGATTACAG                                                    >  SRR4302545.10338114/1‑94 (MQ=60)
                                          CGGGGAGTTATGCCTTCTTTACCATTTCTGTAAGAGAT‑AAAAAAGGCCGGA‑GCATGCTCCGGCCTT‑CGTTTTCATCACTGTGTTTTGATTACAG                                                    <  SRR4302545.8125684/94‑1 (MQ=60)
                                           GGGGAGTTATGCCTTCTTTACCATTTCTGTAAGAGAT‑AAAAAAGGCCGGA‑GCATGCTCCGGCCTT‑CGTTTTCATCACTGTGTTTTGATTACAGC                                                   <  SRR4302545.8629770/94‑1 (MQ=60)
                                            GGGAGTTATGCCTTCTTTACCATTTCTGTAAGAGAT‑AAAAAAGGCCGGA‑GCATGCTCCGGCCTT‑CGTTTTCATCATTGTGTTTTGATTACAGCG                                                  <  SRR4302545.4510843/94‑1 (MQ=60)
                                              GAGTTATGCCTTCTTTACCATTTCTGTAAGAGAT‑AAAAAAGGCCGGA‑GCATGCTCCGGCCTT‑CGTTTTCATCACTGTGTTTTGATTACAGCGCG                                                <  SRR4302545.4260005/94‑1 (MQ=60)
                                               AGTTATGCCTTCTTTACCATTTCTGTAAGAGAT‑AAAAAAGGCCGGA‑GCATGCTCCGGCCTT‑CGTTTTCATCACTGTGTTTTGATTACAGCGCGG                                               <  SRR4302545.7590139/94‑1 (MQ=60)
                                               AGTTATGCCTTCTATACCATTTCTGTAAGAGAT‑AAAAAAGGCCGGA‑GCATGCTCCGGCCTT‑CGTTTTCATCACTGTGTTATGATTACAGCGCGG                                               <  SRR4302545.7590138/94‑1 (MQ=60)
                                                GTTATGCCTTCTTTACCATTTCTGTAAGAGAT‑AAAAAAGGCCGGA‑GCATGCTCCGGCCTT‑CGTTTTCATCACTGTGTTTTGATTACAGCGCGGC                                              <  SRR4302545.1812145/94‑1 (MQ=60)
                                                   ATGCCTTCTTTACCATTTCTGTAAGAGAT‑AAAAAAGGCCGGA‑GCATGCTCCGGCCTT‑CGTTTTCATCACTGTGTTTTGATTACAGCGCGGCGAT                                           <  SRR4302545.7395184/94‑1 (MQ=60)
                                                     GCCTTCTTTACCATTTCTGTAAGAGAT‑AAAAAAGGCCGGA‑GCATGCTCCGGCCTT‑CGTTTTCATCACTGTGTTTTGATTACAGCGCGGCGATAA                                         <  SRR4302545.1270003/94‑1 (MQ=60)
                                                     GCCTTCTTTACCATTTCTGTAAGAGAT‑AAAAAAGGCCGGA‑GCATGCTCCGGCCTT‑CGTTTTCATCACTGTGTTTTGATTACAGCGCGGCGATAA                                         >  SRR4302545.9300954/1‑94 (MQ=60)
                                                      CCTTCTTTACCATTTCTGTAAGAGAT‑AAAAAAGGCCGGA‑GGATGCTCCGGGCTT‑CGTTTTCATTACTGTGTTTTTATTACAGCGCGGGGATAAA                                        >  SRR4302545.8037604/1‑94 (MQ=47)
                                                      CCTTCTTTACCATTTCTGTAAGAGAT‑AAAAAAGGCCGGA‑GCATGCTCCGGCCTT‑CGTTTTCATCACTGTGTTTTGATTACAGCGCGGCGATAAC                                        >  SRR4302545.10308951/1‑94 (MQ=60)
                                                      CCTTCTTTACCATTTCTGTAAGAGAT‑AAAAAAGGCCGGA‑GCATGCTCCGGCCTT‑CGTTTTCATCACTGTGTTTTGATTACAGCGCGGCGATAAC                                        >  SRR4302545.2557249/1‑94 (MQ=60)
                                                      CCTTCTTTACCATTTCTGTAAGAGAT‑AAAAAAGGCCGGA‑GCATGCTCCGGCCTT‑CGTTTTCATCACTGTGGTTTGATTACAGCGCGGCGATAAC                                        >  SRR4302545.7416427/1‑94 (MQ=60)
                                                      CCTTCTTGACCATTTCTGTAAGAGAT‑AAAAAAGGCCGGA‑GCATGCTCCGGCCTT‑CGTTTTCATCACTGTGTTTTGATTACAGCGCGGCGATAAC                                        <  SRR4302545.10123296/94‑1 (MQ=60)
                                                        TTCTTTACCATTTCTGTAAGAGAT‑AAAAAAGGCCGGA‑GCATGCTCCGGCCTT‑CGTTTTCATCACTGTGTTTTGATTACAGCGCGGCGATAACAG                                      >  SRR4302545.9055283/1‑94 (MQ=60)
                                                        TTCTTTACCATTTCTGTAAGAGAT‑AAAAAAGGCCGGA‑GCATGCTCCGGCCTT‑CGTTTTCATCACTGTGTTTTGATTACAGCGCGGCGATAACAG                                      >  SRR4302545.1912025/1‑94 (MQ=60)
                                                        TTCTTTACCATTTCTGTAAGAGAT‑AAAAAAGGCCGGA‑GCATGCTCCGGCCTT‑CGTTTTCATCACTGTGTTTTGATTACAGCGCGGCGATAACAG                                      <  SRR4302545.5696386/94‑1 (MQ=60)
                                                         TCTGTACCATGTCTGTAAGAGAT‑AAAAAAGGCCGGA‑GCATGCTCCGGCCTT‑CGTTTTCATCACTGTGTTTTGATTACAGCGCGGCGATAACAGC                                     <  SRR4302545.6650533/94‑1 (MQ=60)
                                                          CTTTACCATTTCTGTAAGAGAT‑AAAAAAGGCCGGA‑GCATGCTCCGGCCTT‑CGTTTTCATCACTGTGTTTTGATTACAGCGCGGCGATAACAGCC                                    <  SRR4302545.8227395/94‑1 (MQ=60)
                                                          CTTTACCATTTCTGTAAGAGAT‑AAAAAAGGCCGGA‑GCATGCTCCGGCCTT‑CGTTTTCATCACTGTGTTTTGATTACAGCGCGGCGATAACAGCC                                    >  SRR4302545.2340519/1‑94 (MQ=60)
                                                           TTTACCATTTCTGTAAGAGAT‑AAAAAAGGCCGGA‑GCATGCTCCGGCCTT‑CGTTTTCATCACTGTGTTTTGATTACAGCGCGGCGATAACAGCCT                                   <  SRR4302545.8976613/94‑1 (MQ=60)
                                                           TTTACCATTTCTGTAAGAGAT‑AAAAAAGGCCGGA‑GCATGCTCCGGCCTT‑CGTTTTCATCACTGTGTTTTGATTACAGCGCGGCGATAACAGCCT                                   <  SRR4302545.3991593/94‑1 (MQ=60)
                                                           TTTACCATTTCTGTAAGAGAT‑AAAAAAGGCCGGA‑GCATGCTCCGGCCTT‑CGTTTTCATCACTGTGTTTTGATTACAGCGCGGCGATAACAGCCT                                   >  SRR4302545.6299308/1‑94 (MQ=60)
                                                           TTTACCATTTCTGTAAGAGAT‑AAAAAAGGCCGGA‑GCATGCTCCGGCCTT‑CGTTTTCATCACTGTGTTTTGATTACAGCGCGGCGATAACAGCCT                                   >  SRR4302545.9133973/1‑94 (MQ=60)
                                                           TTTACCATTTCTGTAAGAGAT‑AAAAAAGGCCGGA‑GCATGCTCCGCCCTT‑CGTTTTCATCACTGTGTTTTGATTACAGCGCGGCGATAACAGCCT                                   >  SRR4302545.9133974/1‑94 (MQ=60)
                                                           TTTACAATTTCTGTAAGAGAT‑AAAAAAGGCCGGA‑GCATGCTCCGGCCTT‑CGTTTTCATCACTGTGTTTTGATTACAGCGCGGCGATAACAGCCT                                   <  SRR4302545.4289211/94‑1 (MQ=60)
                                                           TTGACCATTTCTGTAAGAGAT‑AAAAAAGGCCGGA‑GCATGCTCCGGCCTT‑CGTTTTCATCACTGTGTTTTGATTACAGCGCGGCGATAACAGCCT                                   <  SRR4302545.10322451/94‑1 (MQ=60)
                                                            TTACCATTTCTGTAAGAGAT‑AAAAAAGGCCGGA‑GCATGCTCCGGCCTT‑CGTTTTCATCACTGTGTTTTGATTACAGCGCGGCGATAACAGCCTG                                  <  SRR4302545.9696707/94‑1 (MQ=60)
                                                            TTACCATTTCTGTAAGAGAT‑AAAAAAGGCCGGA‑GCATGCTCCGGCCTT‑CGTTTTCATCACTGTGTTTTGATTACAGCGCGGCGATAACAGCCTG                                  <  SRR4302545.3245349/94‑1 (MQ=60)
                                                             TACCATTTCTGTAAGAGAT‑AAAAAAGGCCGGA‑GCATGCTCCGGCCTT‑CGTTTTCATCACTGTGTTTTGATTACAGCGCGGCGATAACAGCCTGC                                 >  SRR4302545.1092284/1‑94 (MQ=60)
                                                              ACCATTTCTGTAAGAGAT‑AAAAAAGGCCGGA‑GCATGCTCCGGCCTT‑CGTTTTCATCACTGTGTTTTGATTACAGCGCGGCGATAACAGCCTGCT                                <  SRR4302545.5113549/94‑1 (MQ=60)
                                                              ACCATTTCTGTAAGAGAT‑AAAAAAGGCCGGA‑GCATGCTCCGGCCTT‑CGTTTTCATCACTGTGTTTTGATTACAGCGCGGCGATAACAGCCTGCT                                >  SRR4302545.3095858/1‑94 (MQ=60)
                                                               CCATTTCTGTAAGAGAT‑AAAAAAGGCCGGA‑GCATGCTCCGGCCTT‑CGTTTTCATCACTGTGTTTTGATTACAGCGCGGCGATAACAGCCTGCTG                               <  SRR4302545.128743/94‑1 (MQ=60)
                                                               CCATTTCTGTAAGAGAT‑AAAAAAGGCCGGA‑GCATGCTCCGGCCTT‑CGTTTTCATCACTGTGTTTTGATTACAGCGCGGCGATAACAGCCTGCTG                               <  SRR4302545.7199808/94‑1 (MQ=60)
                                                               CCATTTCTGTAAGAGAT‑AAAAAAGGCCGGA‑GCATGCTCCGGCCTT‑CGTTTTCATCACTGTGTTTTGATTACAGCGCGGCGATAACAGCCTGCTG                               <  SRR4302545.2312412/94‑1 (MQ=60)
                                                               CCATTTCTGTAAGAGAT‑AAAAAAGGCCGGA‑GCATGCTCCGGCCTT‑CGTTTTCATCACTGTGTTTTGATTACAGCGCGGCGATAACAGCCTGCTG                               >  SRR4302545.9040535/1‑94 (MQ=60)
                                                                CATTTCTGTAAGAGAT‑AAAAAAGGCCGGA‑GCATGCTCCGGCCTT‑CGTTTTCATCACTGTGTTTTGATTACAGCGCGGCGATAACAGCCTGCTGT                              <  SRR4302545.8565225/94‑1 (MQ=60)
                                                                 ATTTCTGTAAGAGAT‑AAAAAAGGCCGGA‑GCATGCTCCGGCCTT‑CGTTTTCATCACTGTGTTTTGATTACAGCGCGGCGATAACAGCCTGCTGTT                             <  SRR4302545.4029663/94‑1 (MQ=60)
                                                                 ATTTCTGTAAGAGAT‑AAAAAAGGCCGGA‑GCATGCTCCGGCCTT‑CGTTTTCATCACTGTGTTTTGATTACAGCGCGGCGATAACAGCCTGCTGTT                             >  SRR4302545.1265281/1‑94 (MQ=60)
                                                                  TTTCTGTAAGAGAT‑AAAAAAGGCCGGA‑GCATGCTCCGGCCTT‑CGTTTTCATCACTGTGTTTTGATTACAGCGCGGCGATAACAGCCTGCTGTTC                            >  SRR4302545.1629322/1‑94 (MQ=60)
                                                                  TTTCTGTAAGAGAT‑AAAAAAGGCCGGA‑GCATGCTCCGGCCTT‑CGTTTTCATCACTGTGTTTTGATTACAGCGCGGCGATAACAGCCTGCTGTTC                            >  SRR4302545.6542702/1‑94 (MQ=60)
                                                                   TTCTGTAAGAGAT‑AAAAAAGGCCGGA‑GCATGCTCCGGCCTT‑CGTTTTCATCACTGTGTTTTGATTACAGCGCGGCGATAACAGCCTGCTGTTCA                           >  SRR4302545.1682285/1‑94 (MQ=60)
                                                                   TTCTGTAAGAGAT‑AAAAAAGGCCGGA‑GCATGCTCCGGCCTT‑CGTTTTCATCACTGTGTTTTGATTACAGCGCGGCGATAACAGCCTGCTGTTCA                           <  SRR4302545.9833526/94‑1 (MQ=60)
                                                                   TTCTGTAAGAGA‑AAAAAAAGGCCGGA‑GCATGCTCCGGCCTT‑CGTTTTCATCACTGTGTTTTGATTACAGCGCGGCGATAACAGCCTGCTGTTCA                           <  SRR4302545.2093529/94‑1 (MQ=60)
                                                                     CTGTAAGAGAT‑AAAAAAGGCCGGA‑GCATGCTCCGGCCTT‑CGTTTTCATCACTGTGTTTTGATTACAGCGCGGCGATAACAGCCTGCTGTTCAAT                         >  SRR4302545.1714998/1‑94 (MQ=60)
                                                                     CTGTAAGAGAT‑AAAAAAGGCCGGA‑GCATGCTCCGGCCTT‑CGTTTTCATCACTGTGTTTTGATTACAGCGCGGAGATAACAGCCTGCTGTTCAAT                         >  SRR4302545.10012994/1‑94 (MQ=60)
                                                                      TGTAAGAGAT‑AAAAAAGGCCGGA‑GCATGCTCCGGCCTT‑CGTTTTCATCACTGTGTTTTGATTACAGCGCGGCGATAACAGCCTGCTGTTCAATC                        <  SRR4302545.10542209/94‑1 (MQ=60)
                                                                      TGTAAGAGAT‑AAAAAAGGCCGGA‑GCATGCTCCGGCCTT‑CGTTTTCATCACTGTGTTTTGATTACAGCGCGGCGATAACAGCCCCCTGTTCAATC                        >  SRR4302545.10591462/1‑94 (MQ=60)
                                                                       GTAAGAGAT‑AAAAAAGGCCGGA‑GCATGCTCCGGCCTT‑CGTTTTCATCACTGTGTTTTGATTACAGCGCGGCGATAACAGCCTGCTGTTCAATCA                       >  SRR4302545.1105584/1‑94 (MQ=60)
                                                                       GTAAGAGAT‑AAAAAAGGCCGGA‑GCATGCTCCGGCCTT‑CGTTTTCATCACTGTGTTTTGATTACAGCGCGGCGATAACAGCCTGCTGTTCAATCA                       >  SRR4302545.3403170/1‑94 (MQ=60)
                                                                        TAAGAGAT‑AAAAAATGCCGGA‑GCATGCTCCGGCCTT‑CGTTTTCATCACTGTGTTTTGATTACAGCGCGGCGATAACAGCCTGCTGTTCAATCAG                      <  SRR4302545.620783/94‑1 (MQ=60)
                                                                        TAAGAGAT‑AAAAAAGGCCGGA‑GCATGCTCCGGCCTT‑CGTTTTCATCACTGTGTTTTGATTACAGCGCGGCGATAACAGCCTGCTGTTCAATCAG                      <  SRR4302545.3642935/94‑1 (MQ=60)
                                                                         AAGAGAT‑AAAAAAGGCCGGA‑GCATGCTCCGGCCTT‑CGTTTTCATCACTGTGTTTTGATTACAGCGCGGCGATAACAGCCTGCTGTTCAATCAGT                     >  SRR4302545.8623278/1‑94 (MQ=60)
                                                                          AGAGATAAAAAAGGGCGGG‑‑GCATGCTCCCGGCTTCCG‑TTTCATCACTGTGTTTTGATTTCAGCGCGGCGATAAAAGCCTGCTGTTTAATCAGAT                    >  SRR4302545.2295608/1‑94 (MQ=33)
                                                                          AGAGAT‑AAAAAAGGCCGGA‑GCATGCTCCGGCCTT‑CGTTTTCATCACTGTGTTTTGATTACAGCGCGGCGATAACAGCCTGCTGTTCAATCAGTT                    >  SRR4302545.5067622/1‑94 (MQ=60)
                                                                           GAGAT‑AAAAAAGGCCGGA‑GCATGCTCCGGCCTT‑CGTTTTCATCACTGTGTTTTGATTACAGCGCGGCGATAACAGCCTGCTGTTCAATCAGTTT                   <  SRR4302545.2745127/94‑1 (MQ=60)
                                                                           GAGAT‑AAAAAAGGCCGGA‑GCATGCTCCGGCCTT‑CGTTTTCATCACTGTGTTTTGATTACAGCGCGGCGATAACAGCCTGCTGTTCAATCAGTTT                   >  SRR4302545.5313225/1‑94 (MQ=60)
                                                                            aagatAAAAAAGGCCGGA‑GCATGCTCCGGCCTT‑CGTTTTCATCACTGTGTTTTGATTACAGCGCGGCGATAACAGCCTGCTGTTCAATCAGTTT                   <  SRR4302545.2584214/89‑1 (MQ=60)
                                                                             GAT‑AAAAAAGGCCGGA‑GCATGCTCCGGCCTT‑CGTTTTCATCACTGTGTTTTGATTACAGCGCGGCGATAACAGCCTGCTGTTCAATCAGTTTCG                 <  SRR4302545.10691702/94‑1 (MQ=60)
                                                                               atAAAAAAGGCCGGA‑GCATGCTCCGGCCTT‑CGTTTTCATCTCTGTGTTTTGATTACAGCGCGGCGATAACAGCCTGCTGTTCAATCAGTTTCGC                <  SRR4302545.7435344/92‑1 (MQ=60)
                                                                               atAAAAAAGGCCGGA‑GCATGCTCCGGCCTT‑CGTTTTCATCACTGTGTTTTGATTACAGCGCGGCGATAACAGCCTGCTGTTCAATCAGTTTCGC                >  SRR4302545.7132018/3‑94 (MQ=60)
                                                                               atAAAAAAGGCCGGA‑GCATGCTCCGGCCTT‑CGTTTTCATCACTGTGTTTTGATTACAGCGCGGCGATAACAGCCTGCTGTTCAATCAGTTTCGC                >  SRR4302545.7132017/3‑94 (MQ=60)
                                                                                TAAAAAAGGCCGGA‑GCATGCTCCGGCCTT‑CGTTTTCATCACTGTGTTTTGATTACAGCGCGGCGATAACAGCCTGCTGTTCAATCAGTTTCGCT               <  SRR4302545.5024672/94‑1 (MQ=60)
                                                                                 AAAAAAGGCCGGA‑GCATGCTCCGTCCTT‑CGTTTTCATCACTGTGTTTTGATTACAGCGCGGCGATAACAGCCTGCTGTTCAATCAGTTTCGCTT              <  SRR4302545.7404722/94‑1 (MQ=60)
                                                                                 AAAAAAGGCCGGA‑GCATGCTCCGGCCTT‑CGTTTTCATTACTGTGTTTTTATTACAGCGCGGCGATAACAGCCCGCTGTTCTATCCGTTTCTCTT              >  SRR4302545.6257659/1‑94 (MQ=56)
                                                                                 AAAAAAGGCCGGA‑GCATGCTCCGGCCTT‑CGTTTTCATCGCTGTGTTTTGATTCCAGCGCGGCGATAACAGCCTGCTGTTCAATCAGTTTCGCTT              <  SRR4302545.7404721/94‑1 (MQ=60)
                                                                                 AAAAAAGGCCGGA‑GCATGCTCCGGCCTT‑CGTTTTCATCACTGTGTTTTGATTACAGCGCGGCGATAACAGCCTGCTGTTCAATCAGTTTCGCTT              <  SRR4302545.1601845/94‑1 (MQ=60)
                                                                                 AAAAAAGGCCGG‑GGCATGCTCCCGCCTT‑CGTTTTCATCACTGTGTTTTTATTTCAGCGCCGCCATAACCACCTGCTGTTCAATCAGTTTTGCTT              >  SRR4302545.6257658/1‑94 (MQ=24)
                                                                                  AAAAAGGCCGGA‑GCGTGCTCCGGCCCT‑CGGTTTTATCCCTGTGTTTTGATTACAGCCCGGCGATAACAGACTGCTGTTCAATCAGTTTCGCTTT             >  SRR4302545.6070380/1‑94 (MQ=45)
                                                                                  AAAAAGGCCGGA‑GCATGCTCCGGGCTT‑CGTTTTCATCACTGTGTTTTGATTACAGCGCGGCGATAACAGCCTGCTGTTTAATCAGTTTCGCTTT             <  SRR4302545.8630998/94‑1 (MQ=60)
                                                                                  AAAAAGGCCGGA‑GCATGCTCCGGCCTT‑CGTTTTCATCACTGTGTTTTGATTACAGCGCGGCGATAACAGCCTGCTGTTCAATCAGTTTCGCTTT             >  SRR4302545.1354540/1‑94 (MQ=60)
                                                                                  AAAAAGGCCGGA‑GCATGCTCCGGCCTT‑CGTTTTCATCACTGTGTTTTGATTACAGCGCGGCGATAACAGCCTGCTGTTCAATCAGTTTCGCTTT             >  SRR4302545.5332556/1‑94 (MQ=60)
                                                                                  AAAAAGGCCGGA‑GCATGCTCCGGCCTT‑CGTTTTCATCACTGTGTTTTGATTACAGCGCGGCGATAACAGCCTGCTGTTCAATCAGTTTCGCTTT             <  SRR4302545.9609211/94‑1 (MQ=60)
                                                                                  AAAAAGGCCGGA‑GCATGCTCCGGCCTT‑CGTTTTCATCACTGTGTTTTGATTACAGCGCGGCGATAACAGCCTGCTGTTCAATCAGTTTCGCTTT             <  SRR4302545.9609212/94‑1 (MQ=60)
                                                                                  AAAAAGGCCGG‑GGCATGCTCCGGCCTT‑CGATTTCATCACTGTGTTTTGATTACAGCGCGGAGATAACAGACTGCTGTTCAATCAGTTTCGCTTT             >  SRR4302545.6319218/1‑94 (MQ=60)
                                                                                   AAAAGGCCGGA‑GGATGCTCCGGCCTT‑CGTTTTCATCACTGTGGTTTTATTACAGCGCGGCGATAACAGCCCGCTGTTCCATCCATTTCGCTTTC            >  SRR4302545.80882/1‑94 (MQ=43)
                                                                                   AAAAGGCCGGA‑GCATGCTCCGGCCTT‑CGTTTTCATCACTGTGTTTTGATTACAGCGCGGCGATAACAGCCTGCTGTTCAATCAGTTTCGCTTTC            >  SRR4302545.5776126/1‑94 (MQ=60)
                                                                                   AAAAGGCCGGA‑GCATGCTCCGGCCTT‑CGTTTTCATCACTGTGTTTTGATTACAGCGCGGCGATAACAGCCTGCTGTTCAATCAGTTTCGCTTTC            <  SRR4302545.4841110/94‑1 (MQ=60)
                                                                                   AAAAGGCCGGA‑GCATGCTCCGGCCTT‑CGTTTTCATCACTGTGTTTTGATTACAGCGCGGCGATAACAGCCTGCTGTTCAATCAGTTTCGCTTTC            >  SRR4302545.647852/1‑94 (MQ=60)
                                                                                   AAAAGGCCGGA‑GCATGCTCCGGCCTT‑CGTTTTCATCACTGTGTTTTGATTACAGCGCGGCGATAACAGCCTGCTGTTCAATCAGTTTCGCTTTC            >  SRR4302545.1579709/1‑94 (MQ=60)
                                                                                   AAAAGGCCGGA‑GCATGCTCCGGCCTT‑CGGTTTTATCACTGTGTTTTGATTACAGCGCGGCGATTACAGCCTGGTGTTCAATCAGTTTCGCTTTG            >  SRR4302545.80597/1‑94 (MQ=60)
                                                                                     AAGGGCGGA‑GCATGCTCCGGCCTT‑CGTTTTCATCACTGTGTTTTGATTACAGCGCGGCGATAACAGCCTGCTGTTCAATCAGTTTCGCTTTCGC          >  SRR4302545.10675140/1‑94 (MQ=60)
                                                                                     AAGGCCGGA‑GCATGCTCCGGCCTT‑CGTTTTCATCACTGTGTTTTGATTACAGCGCGGCGATAACAGCCTGGTGTTCAATCAGTTTCGCTTTCGC          >  SRR4302545.5982594/1‑94 (MQ=60)
                                                                                     AAGGCCGGA‑GCATGCTCCGGCCTT‑CGTTTTCATCACTGTGTTTTGATTACAGCGCGGCGATAACAGCCTGCTGTTCAATCAGTTTCGCTTTCGC          >  SRR4302545.8926659/1‑94 (MQ=60)
                                                                                     AAGGCCGGA‑GCATGCTCCGGCCTT‑CGTTTTCATCACTGTGTTTTGATTACAGCGCGGCGATAACAGCCTGCTGTTCAATCAGTTTCGCTTTCGC          >  SRR4302545.3933589/1‑94 (MQ=60)
                                                                                      AGGCCGGA‑GCATGCTCCGGCCTT‑CGTTTTCATCACTGTGTTTTGATTACAGCGCGGCGATAACAGCCTGCTGTTCAATCAGTTTCGCTTTCGCT         >  SRR4302545.794047/1‑94 (MQ=60)
                                                                                       GGCCGGA‑GCATGCTCCGGCCTT‑CGTTTTCATCACTGTGTTTTGATTACAGCGCGGCGATAACAGCCTGCTGTTCAATCAGTTTCGCTTTCGCTT        <  SRR4302545.1199190/94‑1 (MQ=60)
                                                                                        GCCGGA‑GCATGCTCCGGCCTT‑CGTTTTCATCACTGTGTTTTGATTACAGCGCGGCGATAACAGCCTGCTGTTCAATCAGTTTCGCTTTCGCTTC       <  SRR4302545.8869553/94‑1 (MQ=60)
                                                                                         CCGGA‑GCTTGCTCCGGCCTT‑CGTTTTCATCACTGTGTTTTGATTACAGCGCGGCGATAACAGCCTGCTGTTCAATCAGTTTCGCTTTCGCTTCC      <  SRR4302545.4640493/94‑1 (MQ=60)
                                                                                         CCGGA‑GCATGCTCCGGCCTT‑CGTTTTCATCACTGTGTTTTGATTACAGCGCGGCGATAACAGCCTGCTGTTCAATCAGTTTCGCTTTCGCTTCC      <  SRR4302545.4843975/94‑1 (MQ=60)
                                                                                          CGGA‑GCATGCTCCGGCCTT‑CGTTTTCATCACTGTGTTTTGATTACAGCGCGGCGATAACAGCCTGCTGTTCAATCAGTTTCGCTTTCGCTTCCG     >  SRR4302545.6147124/1‑94 (MQ=60)
                                                                                          CGGA‑GCATGCTCCGGCCTT‑CGTTTTCATCACTGTGTTTTGATTACAGCGCGGCGATAACAGCCTGCTGTTCAATCAGTTTCGCTTTCGCTTCCG     <  SRR4302545.10880010/94‑1 (MQ=60)
                                                                                          CGGA‑GCATGCTCCGGCCTT‑CGTTTTCATCACTGTGTTTTGATTACAGCGCGGCGATAACAGCCTGCTGTTCAATCAGTTTCGCTTTCGCTTCCG     >  SRR4302545.1974184/1‑94 (MQ=60)
                                                                                          CGGA‑GCATGCTCCGGCCTT‑CGTTTTCATCACTGTGTTTTGATTACAGCGCGGCGATAACAGCCTGCTGTTCAATCAGTTTCGCTTTCGCTTCCG     >  SRR4302545.3830464/1‑94 (MQ=60)
                                                                                          CGGA‑GCATGCTCCGGCCTT‑CGTTTTCATCACTGTGTTTTGATTACAGCGCGGCGATAACAGCCTGCTGTTCAATCAGTTTCGCTTTCGCTTCCG     <  SRR4302545.4977636/94‑1 (MQ=60)
                                                                                          CGGA‑GCATGCTCCGGCCTT‑CGTTATCATCACTGTGTTTTGATTACAGCGCGGCGATAACAGCCTGCTGTTCAATCAGTTTCGCTTTCGCTTCCG     >  SRR4302545.6908362/1‑94 (MQ=60)
                                                                                           GGA‑GCATGCTCCGGCCTT‑CGTTTTCATCACTGTGTTTTGATTACAGCGCGGCGATAACAGCCTGCTGTTCAATCAGTTTCGCTTTCGCTTCCGC    >  SRR4302545.8560454/1‑94 (MQ=60)
                                                                                           GGA‑GCATGCTCCGGCCTT‑CGTTTTCATCACTGTGTTTTGATTACAGCGCGGCGATAACAGCCTGCTGTTCAATCAGTTTCGCTTTCGCTTCCGC    <  SRR4302545.9322217/94‑1 (MQ=60)
                                                                                           GGA‑GCATGCTCCGGCCTT‑CGTTTTCATCACTGTGTTTTGATTACAGCGCGGCGATAACAGCCTGCTGTTCAATCAGTTTCGCTTTCGCTTCCGC    >  SRR4302545.2084810/1‑94 (MQ=60)
                                                                                           GGA‑GCATGCTCCGGCCTT‑CGTTTTCATCACTGTGTTTTGATTACAGCGCGGCGATAACAGCCTGCTGTTCAATCAGTTTCGCTTTCGCTTCCGC    >  SRR4302545.3546678/1‑94 (MQ=60)
                                                                                           GGA‑GCATGCTCCGGCCTT‑CGTTTTCATCACTGTGTTTTGATTACAGCGCGGCGATAACAGCCTGCTGTTCAATCAGTTTCGCTTTCGCTTCCGC    >  SRR4302545.3546677/1‑94 (MQ=60)
                                                                                           GGA‑GCATGCTCCGGCCTT‑CGTTTTCATCACTGTGTTTTGATTACAGCGCGGCGATAACAGCCTGCTGTCCAATCAGTTTCGCTTTCGCTTCCGC    >  SRR4302545.8237400/1‑94 (MQ=60)
                                                                                           GGA‑GCATGCTCCGGCCTT‑CGATTTCATCACTGTGTTTTGATTACAGCGCGGCGATAACAGCCTGCTGTTCTATCAGTTTCGCTTTCGCTTCCGA    >  SRR4302545.7448539/1‑94 (MQ=60)
                                                                                              |                                                                                              
AAAGAAGAACAACCAGATAAAGGCTTCTTAGCCAGTATTTCACGGGGAGTTATGCCTTCTTTACCATTTCTGTAAGAGATAAAAAAAGGCCGGATGCATGCTCCGGCCTTGCGTTTTCATCACTGTGTTTTGATTACAGCGCGGCGATAACAGCCTGCTGTTCAATCAGTTTCGCTTTCGCTTCCGCAT  >  NC_000913/4478417‑4478605

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 2 ≤ ATCG/ATCG < 23 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑