Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I1 R1 647 270.1 21258240 98.4% 20918108 94.0

Breseq alignment

N/A

GATK/CNVnator alignment

BRESEQ :: bam2aln output
GCGATCGCGTCAACGTCAGTTTCTCTACCTTAACCTGTCTGGAAGTGATGGCGGGCGGCGTTTC‑AAA‑GGCCATGCGCTGGAAG‑GCTGGCG‑‑‑‑AACT‑GGCTACAG‑CTGAAGGATTG‑ATTGCGTT‑‑GTGACGGGATGAACGACGCCGAAATGCTGTCG  >  NC_000913/4008309‑4008461
                                                                                            |                                                                        
GCGATCGCGTCAACGTCAGTTTCTCTACCTTAACCTGTCTGGAAGTGATGGCGGGCGGCGTTTCAAAA‑GGCCATGCGCTGGAAGCGGTGGCG‑‑‑‑AA                                                                    <  SRR4302545.2885434/94‑1 (MQ=60)
GCGATCGCGTCAACGTCAGTTTCTCTACCTTAACCTGTCTGGAAGTGATGGCGGGCGGCGTTTCAAAA‑GGCCATGCGCTGGAAGCGGTGGCG‑‑‑‑AA                                                                    <  SRR4302545.5624836/94‑1 (MQ=60)
GCGATCGCGTCAACGTCAGTTTCTCTACCTTAACCTGTCTGGAAGTGATGGCGGGCGGCGTTTC‑AAAGGGCCATGCGCTGGAAGCGGTGGCG‑‑‑‑AA                                                                    <  SRR4302545.589885/94‑1 (MQ=60)
                                                              tcaAAA‑GGCCATGCGCTGGAAGCGGTGGCGAAGAAACTGGGCTACAGCCTGAAGGATTGTATTGCGTTTGGTGACGGGATGAACGACGCCGAAA          >  SRR4302545.4767244/4‑94 (MQ=16)
                                                               caAAA‑GGCCATGCGCTGGAAGCGGTGGCGAAGAAACTGGGCTACAGCCTGAAGGATTGTATTGCGTTTGGTGACGGGATGAACGACGCCGAAAT         <  SRR4302545.8579375/92‑1 (MQ=18)
                                                               caAAA‑GGCCATGCGCTGGAAGCGGTGGCGAAGAAACTGGGCTACAGCCTGAAGGATTGTATTGCGTTTGGTGACGGGATGAACGACGCCGAAAT         <  SRR4302545.8480524/92‑1 (MQ=18)
                                                               caAAA‑GGCCATGCGCTGGAAGCGGTGGCGAAGAAACTGGGCTACAGCCTGAAGGATTGTATTGCGTTTGGTGACGGGATGAACGACGCCGAAAT         <  SRR4302545.8435863/92‑1 (MQ=18)
                                                               caAAA‑GGCCATGCGCTGGAAGCGGTGGCGAAGAAACTGGGCTACAGCCTGAAGGATTGTATTGCGTTTGGTGACGGGATGAACGACGCCGAAAT         <  SRR4302545.2348517/92‑1 (MQ=18)
                                                               caAAA‑GGCCATGCGCTGGAAGCGGTGGCGAAGAAACTGGGCTACAGCCTGAAGGATTGTATTGCGTTTGGTGACGGGATGAACGACGCCGAAAT         >  SRR4302545.2354825/3‑94 (MQ=18)
                                                               caAAA‑GGCCATGCGCTGGAAGCGGTGGCGAAGAAACTGGGCTACAGCCTGAAGGATTGTATTGCGTTTGGTGACGGGATGAACGACGCCGAAAT         <  SRR4302545.2575333/92‑1 (MQ=18)
                                                               caAAA‑GGCCATGCGCTGGAAGCGGTGGCGAAGAAACTGGGCTACAGCCTGAAGGATTGTATTGCGTTTGGTGACGGGATGAACGACGCCGAAAT         <  SRR4302545.8387026/92‑1 (MQ=18)
                                                               A‑AAA‑GGCCATGCGCTGGAAGCGGTGGCGAAGAAACTGGGCTACAGCCTGAAGGATTGTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATG        >  SRR4302545.4910112/1‑94 (MQ=19)
                                                               A‑AAA‑GGCCATGCGCTGGAAGCGGTGGCGAAGAAACTGGGCTACAGCCTGAAGGATTGTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATG        >  SRR4302545.874445/1‑94 (MQ=19)
                                                               A‑AAA‑GGCCATGCGCTGGAAGCGGTGGCGAAGAAACTGGGCTACAGCCTGAAGGATTGTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATG        >  SRR4302545.5616317/1‑94 (MQ=19)
                                                               A‑AAA‑GGCCATGCGCTGGAAGCGGTGGCGAAGAAACTGGGCTACAGCCTGAAGGATTGTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATG        >  SRR4302545.3592584/1‑94 (MQ=19)
                                                               A‑AAA‑GGCCATGCGCTGGAAGCGGTGGCGAAGAAACTGGGCTACAGCCTGAAGGATTGTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATG        >  SRR4302545.312860/1‑94 (MQ=19)
                                                                 AAA‑GGCCATGCGCTGGAAGCGGTGGCGAAGAAACTGGGCTACAGCCTGAAGGATTGTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGC       <  SRR4302545.1035173/94‑1 (MQ=21)
                                                                   A‑GGCCATGCGCTGGAAGCGGTGGCGAAGAAACTGGGCTACAGCCTGAAGGATTGTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTG     <  SRR4302545.5627053/94‑1 (MQ=23)
                                                                   A‑GGCCATGCGCTGGAAGCGGTGGCGAAGAAACTGGGCTACAGCCTGAAGGATTGTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTG     >  SRR4302545.287453/1‑94 (MQ=23)
                                                                   A‑GGCCATGCGCTGGAAGCGGTGGCGAAGAAACTGGGCTACAGCCTGAAGGATTGTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTG     <  SRR4302545.10720851/94‑1 (MQ=23)
                                                                     GGCCATGCGCTGGAAGCGGTGGCGAAGAAACTGGGCTACAGCCTGAAGGATTGTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGT    >  SRR4302545.3087452/1‑94 (MQ=25)
                                                                     GGCCATGCGCTGGAAGCGGTGGCGAAGAAACTGGGCTACAGCCTGAAGGATTGTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGT    <  SRR4302545.6870088/94‑1 (MQ=25)
                                                                      GCCATGCGCTGGAAGCGGTGGCGAAGAAACTGGGCTACAGCCTGAAGGATTGTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTC   <  SRR4302545.5377252/94‑1 (MQ=26)
                                                                      GCCATGCGCTGGAAGCGGTGGCGAAGAAACTGGGCTACAGCCTGAAGGATTGTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTC   <  SRR4302545.7325608/94‑1 (MQ=26)
                                                                       CCATGCGCTGGAAGCGGTGGCGAAGAAACTGGGCTACAGCCTGAAGGATTGTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCG  <  SRR4302545.5176382/94‑1 (MQ=28)
                                                                       CCATGCGCTGGAAGCGGTGGCGAAGAAACTGGGCTACAGCCTGAAGGATTGTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCG  <  SRR4302545.1999026/94‑1 (MQ=28)
                                                                       CCATGCGCTGGAAGCGGTGGCGAAGAAACTGGGCTACAGCCTGAAGGATTGTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCG  >  SRR4302545.1430924/1‑94 (MQ=28)
                                                                       CCATGCGCTGGAAGCGGTGGCGAAGAAACTGGGCTACAGCCTGAAGGATTGTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCG  <  SRR4302545.10756070/94‑1 (MQ=28)
                                                                                            |                                                                        
GCGATCGCGTCAACGTCAGTTTCTCTACCTTAACCTGTCTGGAAGTGATGGCGGGCGGCGTTTC‑AAA‑GGCCATGCGCTGGAAG‑GCTGGCG‑‑‑‑AACT‑GGCTACAG‑CTGAAGGATTG‑ATTGCGTT‑‑GTGACGGGATGAACGACGCCGAAATGCTGTCG  >  NC_000913/4008309‑4008461

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑