Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A0 F0 I1 R1 245 579.6 28867798 97.7% 28203838 96.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 4,593,254 2 bp→GC coding (59‑60/807 nt) yjjM ← hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,593,2540CG100.0% 2006.9 / NA 514A20G (GCG→GGC) yjjMhypothetical protein
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base G (253/261);  total (253/261)
*NC_0009134,593,2550GC99.8% 1977.2 / NA 514A20G (GCG→GGC) yjjMhypothetical protein
Reads supporting (aligned to +/- strand):  ref base G (1/0);  new base C (251/261);  total (253/261)
Fisher's exact test for biased strand distribution p-value = 4.91e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 525 total aligned reads displayed.

TTCACTCATTGAAGCGGTAGTACAGGCGAAACCGTCATCATGGTCAGGTTTACGCGCAATAACATAGTCGTTTCCAACCTGCGTCAAGACCGCGCATTCGCGTAAGTGGCTGAGAATATGACGCACTGTGGTGCGGCTGATGTTGTACATTTCCGCCAGCGCACTTTGCGAT‑GAAGTGGG  >  NC_000913/4593164‑4593343
                                                                                          ||                                                                                         
ttCACTCATTGAAGCGGTAGTACAGGCGAAACCGTCATCATGGTCAGGTTTACGCGCAATAACATAGTCGTTTCCAACCTGCGTCAAGACGCCGCa                                                                                       >  1:14107883/1‑96 (MQ=255)
 tCACTCATTGAAGCGGTAGTACAGGCGAAACCGTCATCATGGTCAGGTTTACGCGCAATAACATAGTCGTTTCCAACCTGCGTCAAGACGCCGCAt                                                                                      <  1:8126517/96‑1 (MQ=255)
 tCACTCATTGAAGCGGTAGTACAGGAGAAACCGTCATCATGGTCAGGTTTACGCGCAATAACATAGTCGTTTCCAACCTGCGTCAAGACGCCGCAt                                                                                      >  2:6290106/1‑96 (MQ=255)
  cACTCATTGAAGCGGTAGTACAGGCGAAACCGTCATCATGGTCAGGTTTACGCGCAATAACATAGTCGTTTCCAACCTGCGTCAAGACGCCGCAtt                                                                                     <  1:6815584/96‑1 (MQ=255)
   aCTCATTGACGCGGTAGTACAGGCGAAACCGTCACCATGGTCAGGTTTACGCGCAATAACATAGTCCTTTCCAACCTGCGTCAAGACGCCGCATTc                                                                                    <  1:3255904/96‑1 (MQ=255)
   aCTCATTGAAGCGGTAGTACAGGCGAAACCGTCATCATGGTCAGGTTTACGCGCAATAACATAGTCGTTTCCAACCTGCGTCAAGACGCCGCATTc                                                                                    >  2:600698/1‑96 (MQ=255)
   aCTCATTGAAGCGGTAGTACAGGCGAAACCGTCATCATGGTCAGGTTTACGCGCAATAACATAGTCGTTTCCAACCTGCGTCAAGACGCCGCATTc                                                                                    <  2:4749947/96‑1 (MQ=255)
   aCTCATTGAAGCGGTAGTACAGGCGAAACCGTCATCATGGTCAGGTTTACGCGCAATAACATAGTCGTTTCCAACCTGCGTCAAGACGCCGCATTc                                                                                    <  2:12803396/96‑1 (MQ=255)
    cTCATTGAAGCGGTAGTACAGGCGAAACCGTCATCATGGTCAGGTTTACGCGCAATAACATAGTCGTTTCCAACCTGCGTCAAGACGCCGCATTcg                                                                                   <  1:3759355/96‑1 (MQ=255)
     tCATTGAAGCGGTAGTACAGGCGAAACCGTCATCATGGTCAGGTTTACGCGCAATAACATAGTCGTTTCCAACCTGCGTCAAGACGCCGCATTcgc                                                                                  <  1:2135829/96‑1 (MQ=255)
       aTTGAAGCGGTAGTACAGGCGAAACCGTCATCATGGTCAGGTTTACGCGCAATAACATAGTCGTTTCCAACCTGCGTCAAGACGCCGCATTCGCGt                                                                                <  1:3675005/96‑1 (MQ=255)
         tGAAGCGGTAGTACAGGCGAAACCGTCATCATGGTCAGGTTTACGCGCAATAACATAGTCGTTTCCAACCTGCGTCAAGACGCCGCATTCGCGTaa                                                                              >  1:5935302/1‑96 (MQ=255)
         tGAAGCGGTAGTACAGGCGAAACCGTCATCATGGTCAGGTTTACGCGCAATAACATAGTCGTTTCCAACCTGCGTCAAGACGCCGCATTCGCGTaa                                                                              <  1:5126492/96‑1 (MQ=255)
           aaGCGGTAGTACAGGCGAAACCGTCATCATGGTCAGGTTTACGCGCAATAACATAGTCGTTTCCAACCTGCGTCAAGACGCCGCATTCGCGTAAGt                                                                            <  1:11632346/96‑1 (MQ=255)
             gCGGTAGTACAGGCGAAACCGTCATCATGGTCAGGTTTACGCGCAATAACATAGTCGTTTCCAACCTGCGTCAAGACGCCGCATTCGCGTAAGTgg                                                                          <  2:5289939/96‑1 (MQ=255)
              cGGTAGTACAGGCGAAACCGTCATCATGGTCAGGTTTACGCGCAATAACATAGTCGTTTCCAACCTGCGTCAAGACGCCGCATTCGCGTAAGTGGc                                                                         >  1:7464444/1‑96 (MQ=255)
               ggTAGTACAGGCGAAACCGTCATCATGGTCAGGTTTACGCGCAATAACATAGTCGTTTCCAACCTGCGTCAAGACGCCGCATTCGCGTAAGTGGCt                                                                        <  1:6201390/96‑1 (MQ=255)
                gtagtaCAGGCGAAACCGTCATCATGGTCAGGTTTACGCGCAATAACATAGTCGTTTCCAACCTGCGTCAAGACGCCGCATTCGCGTAAGTGGCTg                                                                       <  1:11003413/96‑1 (MQ=255)
                 tagtaCAGGCGAAACCGTCATCATGGTCAGGTTTACGCGCAATAACATAGTCGTTTCCAACCTGCGTCAAGACGCCGCATTCGCGTAAGTGGCTga                                                                      >  2:1350551/1‑96 (MQ=255)
                  agtaCAGGCGAAACCGTCATCATGGTCAGGTTTACGCGCAATAACATAGTCGTTTCCAACCTGCGTCAAGACGCCGCATTCGCGTAAGTGGCTgag                                                                     >  1:6643838/1‑96 (MQ=255)
                  agtaCAGGAGAAACCGTCATCATGGGCAGGTTTACGCGCAATAACATAGTCGTTTCCAACCTGCGTCAAGACGCCGCATTCGCGTATGATGCTgag                                                                     >  1:5798087/1‑96 (MQ=255)
                   gtaCAGGCGAAACCGTCATCATGGTCAGGTTTACGCGCAATAACATAGTCGTTTCCAACCTGCGTCAAGACGCCGCATTCGCGTAAGTGGCTgaga                                                                    <  2:9681488/96‑1 (MQ=255)
                   gtaCAGGCGAAACCGTCATCATGGTCAGGTTTACGCGCAATAACATAGTCGTTTCCAACCTGCGTCAAGACGCCGCATTCGCGGAAGTGGCTgaga                                                                    >  1:2428637/1‑96 (MQ=255)
                     aCAGGCGAAACCGTCATCATGGTCAGGTTTACGCGCAATAACATAGTCGTTTCCAACCTGCGTCAAGACGCCGCATTCGCGTAAGTGGCTGAGAat                                                                  <  2:8089876/96‑1 (MQ=255)
                      cAGGCGAAACCGTCATCATGGTCAGGTTTACGCGCAATAACATAGTCGTTTCCAACCTGCGTCAAGACGCCGCATTCGCGTAAGTGGCTGAGAata                                                                 <  2:5488434/96‑1 (MQ=255)
                       aGGCGAAACCGTCATCATGGTCAGGTTTACGCGCAATAACATAGTCGTTTCCAACCTGCGTCAAGACGCCGCATTCGCGTAAGTGGCTGAGAatat                                                                >  1:2612582/1‑96 (MQ=255)
                       aGGCGAAACCGTCATCATGGTCAGGTTTACGCGCAATAACATAGTCGTTTCCAACCTGCGTCAAGACGCCGCATTCGCGTAAGTGGCTGAGAatat                                                                >  2:2780573/1‑96 (MQ=255)
                       aGGCGAAACCGTCATCATGGTCAGGTTTACGCGCAATAACATAGTCGTTTCCAACCTGCGTCAAGACGCCGCATTCGCGTAAGTGGCTGAGAatat                                                                >  2:5002650/1‑96 (MQ=255)
                       aGGCGAAACCGTCATCATGGTCAGGTTTACGCGCAATAACATAGTCGTTTCCAACCTGCGTCAAGACGCCGCATTCGCGTAAGTGGCTGAGAatag                                                                >  1:5311344/1‑95 (MQ=255)
                       aGGAGAAACCGTCATCATGGTCAGGTTTACGCGCAATAACATAGTCGTTTCCAACCTGCGTCAAGACGCCGCATTCGCGTAAGTGGCTGAGAatat                                                                >  1:4691430/1‑96 (MQ=255)
                        ggCGAAACCGTCATCATGGTCAGGTTTACGCGCAATAACATAGTCGTTTCCAACCTGCGTCAAGACGCCGCATTCGCGTAAGTGGCTGAGAATATg                                                               >  2:6612636/1‑96 (MQ=255)
                          cGAAACCGTCATCATGGTCAGGTTTACGCGCAATAACATAGTCGTTTCCAACCTGCGTCAAGACGCCGCATTCGCGTAAGTGGCTGAGAATATGAc                                                             >  1:8632349/1‑96 (MQ=255)
                          cGAAACCGTCATCATGGTCAGGTTTACGCGCAATAACAGAGTCGTTTCCAACCTGCGTCAAGACGCCGCATTCGCGTAAGTGGCTGAGAATATGAc                                                             >  2:12615730/1‑96 (MQ=255)
                           gAAACCGTCATCATGGTCAGGTTTACGCGCAATAACATAGTCGTTTCCAACCTGCGTCAAGACGCCGCATTCGCGTAAGTGGCTGAGAATATGACg                                                            >  2:4192291/1‑96 (MQ=255)
                           gAAACCGTCATCATGGTCAGGTTTACGCGCAATAACATAGTCGTTTCCAACCTGCGTCAAGACGCCGCATTCGCGTAAGTGGCTGAGAATATGACg                                                            >  1:8856483/1‑96 (MQ=255)
                            aaaCCGTCATCATGGTCAGGTTTACGCGCAATAACATAGTCGTTTCCAACCTGCGTCAAGACGCCGCATTCGCGTAAGTGGCTGAGAATATGACGc                                                           >  2:8655774/1‑96 (MQ=255)
                             aaCCGTCATCATGCTCAGGTTTACGCGCAATAACATAGTCGTTTCCAACCTGCGTCAAGACGCCGCATTCGCGTAAGTGGCTGAGAATATGACGCa                                                          >  1:13441672/1‑96 (MQ=255)
                              aCCGTCATCATGGTCAGGTTTACGCGCAATAACATAGTCGTTTCCAACCTGCGTCAAGAGGCGGCATTCGCGTAAGTGGCTGAGAATATGACGCAc                                                         >  1:11548336/1‑96 (MQ=255)
                               ccGTCATCATGGTCAGGTTTACGCGCAATAACATAGTCGTTTCCAACCTGCGTCAAGACGCCGCATTCGCGTAAGTGGCTGAGAATATGACGCACt                                                        <  2:380770/96‑1 (MQ=255)
                                cGTCATCATGGTCAGGTTTACGCGCAATAACATAGTCGTTTCCAACCTGCGTCAAGACGCCGCATTCGCGTAAGTGGCTGAGAATATGACGCACtg                                                       <  2:1865421/96‑1 (MQ=255)
                                cGTCATCATGGTCAGGTTTACGCGCAATAACATAGTCGTTTCCAACCTGCGTCAAGACGCCGCATTCGCGTAAGTGGCTGAGAATATGACGCACtg                                                       >  2:11623134/1‑96 (MQ=255)
                                 gTCATCATGGTCAGGTTTACGCGCAATAACATAGTCGTTTCCAACCTGCGTCAAGACGCCGCATTCGCGTAAGTGGCTGAGAATATGACGCACtgt                                                      >  1:634316/1‑96 (MQ=255)
                                 gTCATCATGGTCAGGTTTACGCGCAATAACATAGTCGTTTCCAACCTGCGTCAAGACGCCGCATTCGCGTAAGTGGCTGAGAATATGACGCACtgt                                                      >  1:9150893/1‑96 (MQ=255)
                                     tcatGGTCAGGTTTACGCGCAATAACATAGTCGTTTCCAACCTGCGTCAAGACGCCGCATTCGCGTAAGTGGCTGAGAATATGACGCACTgtggtg                                                  <  1:1308873/96‑1 (MQ=255)
                                      catGGTCAGGTTTACGCGCAATAACATAGTCGTTTCCAACCTGCGTCAAGACGCCGCATTCGCGTAAGTGGCTGAGAATATGACGCACTGTGGTGc                                                 <  2:11460131/96‑1 (MQ=255)
                                      catGGTCAGGTTTACGCGCAATAACATAGTCGTTTCCAACCTGCGTCAAGACGCCGCATTCGCGTAAGTGGCTGAGAATATGACGCACTGTGGTGc                                                 <  1:9740064/96‑1 (MQ=255)
                                       atGGTCAGGTTTACGCGCAATAACATAGTCGTTTCCAACCTGCGTCAAGACGCCGCATTCGCGTAAGTGGCTGAGAATATGACGCACTGTGGTGCg                                                >  2:1008196/1‑96 (MQ=255)
                                        tGGTCAGGTTTACGCGCAATAACATAGTCGTTTCCAACCTGCGTCAAGACGCCGCATTCGCGTAAGTGGCTGAGAATATGACGCACTGTGGTGCgg                                               <  1:7186535/96‑1 (MQ=255)
                                        tGGTCAGGTTTACGCGCAATAACATAGTCGTTTCCAACCTGCGTCAAGACGCAGCATTCGCGTAAGAGGCTGAGAATATTGCGCACTGTGGTGCgg                                               >  1:13938753/1‑96 (MQ=255)
                                         ggTCAGGTTTACGCGCAATAACATAGTCGTTTCCAACCTGCGTCAAGACGCCGCATTCGCGTAAGTGGCTGAGAATATGACGCACTGTGGTGCGGc                                              >  2:7430824/1‑96 (MQ=255)
                                           tCAGGTTTACGCGCAATAACATAGTCGTTTCCAACCTGCGTCAAGACGCCGCATTCGCGTAAGTGGCTGAGAATATGACGCACTGTGGTGCGGCTg                                            >  1:5542894/1‑96 (MQ=255)
                                            cAGGTTTACGCGCAATAACATAGTCGTTTCCAACCTGCGTCAAGACGCCGCATTCGCGTAAGTGGCTGAGAATATGACGCACTGTGGTGCGGCTGa                                           <  2:4356266/96‑1 (MQ=255)
                                             aGGTTTACGCGCAATAACATAGTCGTTTCCAACCTGCGTCAAGACGCCGCATTCGCGTAAGTGGCTGAGAATATGACGCACTGTGGTGCGGCTGAt                                          >  1:10007008/1‑96 (MQ=255)
                                             aGGTTTACGCGCAATAACATAGTCGTTTCCAACCTGCGTCAAGACGCCGCATTCGCGTAAGTGGCTGAGAATATGACGCACTGTGGTGCGGCTGAt                                          <  2:10532733/96‑1 (MQ=255)
                                              ggTTTACGCGCAATAACATAGTCGTTTCCAACCTGCGTCAAGACGCCGCATTCGCGTAAGTGGCTGAGAATATGACGCACTGTGGTGCGGCTGAtg                                         >  2:9006586/1‑96 (MQ=255)
                                                 ttACGCGCAATAACATAGTCGTTTCCAACCTGCGTCAAGACGCCGCATTCGCGTAAGTGGCTGAGAATATGACGCACTGTGGTGCGGCTGAtgttg                                      <  1:11884648/96‑1 (MQ=255)
                                                 ttACGCGCAATAACATAGTCGTTTCCAACCTGCGTCAAGACGCCGCATTCGCGTAAGTGGCTGAGAATATGACGCACTGTGGTGCGGCTGAtgttg                                      >  2:7629253/1‑96 (MQ=255)
                                                 ttACGCGCAATAACATAGTCGTTTCCAACCTGCGTCAAGACGCCGCATTCGCGTAAGTGGCTGAGAATATGACGCACTGTGGTGCGGCTGAtgttg                                      >  2:7244936/1‑96 (MQ=255)
                                                 ttACGCGCAATAACATAGTCGTTTCCAACCTGCGTCAAGACGCCGCATTCGCGTAAGTGGCTGAGAATATGACGCACTGTGGTGCGGCTGAtgttg                                      >  2:14336233/1‑96 (MQ=255)
                                                 ttACGCGCAATAACATAGTCGTTTCCAACCTGCGTCAAGACGCCGCATTCGCGTAAGTGGCTGAGAATATGACGCACTGTGGTGCGGCTGAtgtag                                      >  1:10442882/1‑94 (MQ=255)
                                                  tccgcgCAATAACATAGTCGTTTCCAACCTGCGTCAAGACGCCGCATTCGCGTAAGTGGCTGAGAATATGACGCACTGTGGTGCGGCTGAtgttgt                                     <  2:2247976/94‑1 (MQ=255)
                                                     gcgcAATAACATAGTCGTTTCCAACCTGCGTCAAGACGCCGCATTCGCGTAAGTGGCTGAGAATATGACGCACTGTGGTGCGGCTGATGTTGTACa                                  <  2:12919315/96‑1 (MQ=255)
                                                       gcAATAACATAGTCGTTTCCAACCTGCGTCAAGACGCCGCATTCGCGTAAGTGGCTGAGAAAATGAGGACTTGGGGGGCGGCGGATGTTGTAAAtt                                >  2:315715/1‑96 (MQ=255)
                                                        cAATAACATAGTCGTTTCCAACCTGCGTCAAGACGCCGCATTCGCGTAAGTGGCTGAGAATATGACGCACTGTGGTGCGGCTGATGTTGTACAttt                               <  2:11873809/96‑1 (MQ=255)
                                                         aaTAACATAGTCGTTTCCAACCTGCGTCAAGACGCCGCATTCGCGTAAGTGGCTGAGAATATGACGCACTGTGGTGCGGCTTATGTTGTACATTTc                              >  1:14417799/1‑96 (MQ=255)
                                                         aaTAACATAGTCGTTTCCAACCTGCGTCAAGACGCCGCATTCGCGTAAGTGGCTGAGAATATGACGCACTGTGGTGCGGCTGATGTTGTACAttgc                              <  1:10785110/96‑3 (MQ=255)
                                                         aaTAACATAGTCGTTTCCAACCTGCGTCAAGACGCCGCATTCGCGTAAGTGGCTGAGAATATGACGCACTGTGGTGCGGCTGATGTTGTACATTTc                              >  1:12195307/1‑96 (MQ=255)
                                                          aTAACATAGTCGTTTCCAACCTGCGTCAAGACGCCGCATTCCCGTAAGTGGCTGAGAATATGACGCACTGTGGTGCGGCTGATGTTGTACATTTcc                             >  2:13266814/1‑96 (MQ=255)
                                                           tAACATAGTCGTTTCCAACCTGCGTCAAGACGCCGCATTCGCGTAAGTGGCTGAGATTATGACGCACTGTGGTGCGGATGATGTTGTACATTTCCg                            <  1:12615730/96‑1 (MQ=255)
                                                            aaCATAGTCGTTTCCAACCTGCGTCAAGACGCCGCATTCGCGTAAGTGGCTGAGAATATGACGCACTGTGGTGCGGCTGATGTTGTACATTTCCGc                           >  1:4937025/1‑96 (MQ=255)
                                                             aCATAGTCGTTTCCAACCTGCGTCAAGACGCCGCATTCGCGTAAGTGGCTGAGAATATGACGCACTGTGGTGCGGCTGATGTTGTACATTTCCGcc                          >  1:9946345/1‑96 (MQ=255)
                                                              cATAGTCGTTTCCAACCTGCGTCAAGACGCCGCATTCGCGTAAGTGGCTGAGAATATGACGCACTGTGGTGCGGCTGATGTTGTACATTTCCGCCa                         >  2:1211932/1‑96 (MQ=255)
                                                              cATAGTCGTTTCCAACCTGCGTCAAGACGCCGCATTCGCGTAAGTGGCTGAGAATATGACGCACTGTGGTGCGGCTGATGTTGTACATTTCCGCCa                         <  2:8849827/96‑1 (MQ=255)
                                                               aTAGTCGTTTCCAACCTGCGTCAAGACGCCGCATTCGCGTAAGTGGCTGAGAATATGACGCACTGTGGTGCGGCTGATGTTGTACATTTCCGCCAg                        >  1:7030633/1‑96 (MQ=255)
                                                                tAGTCGTTTCCAACCTGCGTCAAGACGCCGCATTCGCGTAAGTGGCTGAGAATATGACGCACTGTGGTGCGGCTGATGTTGTACATTTCCGCCAgc                       <  2:11224567/96‑1 (MQ=255)
                                                                tAGTCGTTTCCAACCTGCGTCAAGACGCCGCATTCGCGTAAGTGGCTGAGAATATGACGCACTGTGGTGCGGCTGATGTTGTACATTTCCGCCAgc                       <  2:8244299/96‑1 (MQ=255)
                                                                  gTCGTTTCCAACCTGCGTCAAGACGCCGCATTCGCGTAAGTGGCTGAGAATATGACGCACTGTGGTGCGGCTGATGTTGTACATTTCCGCCAgcgc                     <  2:10898832/96‑1 (MQ=255)
                                                                  gTCGTTTCCAACCTGCGTCAAGACGCCGCATTCGCGTAAGTGGCTGAGAATATGACGCACTGTGGTGCGGCTGATGTTGTACATTTCCGCCAgcgc                     <  1:3435921/96‑1 (MQ=255)
                                                                  gTCGTTTCCAACCTGCGTCAAGACGCCGCATTCGCGTAAGTGGCTGAGAATATGACGCACTGTGGTGCGGCTGATGTTGTACATTTCCGCCAgcgc                     >  2:4662240/1‑96 (MQ=255)
                                                                     gTTTCCAACCTGCGTCAAGACGCCGCATTCGCGTAAGTGGCTGAGGATATGACGCACTGTGGTGCGGCTGATGTTGTACATTTCCGCCAGCGCACt                  >  2:6867054/1‑96 (MQ=255)
                                                                     gTTTCCAACCTGCGTCAAGACGCCGCATTCGCGTAAGTGGCTGAGAATATGACGCACTGTGGTGCGGCTGATGTTGTACATTTCCGCCAGCGCACt                  >  2:9181620/1‑96 (MQ=255)
                                                                      tttCCAACCTGCGTCAAGACGCCGCATTCGCGTAAGTGGCTGAGAATATGACGCACTGTGGTGCGGCTGATGTTGTACATTTCCGCCAGCGCACtt                 >  2:2083623/1‑96 (MQ=255)
                                                                        tCCAACCTGCGTCAAGACGCCGCATTCGCGTAAGTGGCTGAGAATATGACGCACTGTGGTGCGGCTGATGTTGTACATTTCCGCCAGCGCACTTTg               <  1:9486505/96‑1 (MQ=255)
                                                                         ccAACCTGCGTCAAGACGCCGCATTCGCGTAAGTGGCTGAGAATATGACGCACTGTGGTGCGGCTGATGTTGTACATTTCCGCCAGCGCACTTTGc              >  1:2788997/1‑96 (MQ=255)
                                                                           aaCCTGCGTCAAGACGCCGCATTCGCGTAAGTGGCTGAGAATATGACGCACTGTGGTGCGGCTGATGTTGTACATTTCCGCCAGCGCACTTTGCGa            <  1:4103867/96‑1 (MQ=255)
                                                                             ccTGCGTCAAGACGCCGCATTCGCGTAAGTGGCTGAGAATATGACGCACTGTGGTGCGGCTGATGTTGTACATTTCCGCCAGCGGACTTTGCGAT‑g         >  1:7808396/1‑96 (MQ=255)
                                                                             ccTGCGTCAAGACGCCGCATTCGCGTAAGTGGCTGAGAATATGACGCACTGTGGTGCGGCTGATGTTGTACATTTCCGCCAGCGCACTTTGCGAT‑g         <  2:12274647/96‑1 (MQ=255)
                                                                              cTGNGTCAAGACGCCGCATTCGCGTAAGTGGCTGAGAATATGACGCACTGTGGTGCGGCTGATGTTGTACATTTCCGCCAGCGCACTTTGCGAT‑gg        >  2:5672318/1‑95 (MQ=255)
                                                                              cTGCGTCAAGACGCCGCATTCGCGTAAGTGGCTGAGAATATGACGCACTGTGGTGCGGCTGATGTTGTACATTTCCGCCAGCGCACTTTGCGAT‑gg        <  2:6360923/96‑2 (MQ=255)
                                                                              cTGCGTCAAGACGCCGCATTCGCGTAAGTGGCTGAGAATATGACGCACTGTGGTGCGGCTGATGTTGTACATTTCCGCCAGCGCACTTTGCGAT‑gg        >  2:346026/1‑95 (MQ=255)
                                                                               tGCGTCAAGACGCCGCATTCGCGTAAGTGGCTGAGAATATGACGCACTGTGGTGCGGCTGATGTTGTACATTTCCGCCAGCGCACTTTGCGAT‑gga       >  1:1915302/1‑94 (MQ=255)
                                                                                gCGTCAAGACGCCGCATTCGCGTAAGTGGCTGAGAATATGACGCACTGTGGTGCGGCTGATGTTGTACATTTCCGCCAGCGCACTTTGCGAT‑ggaa      <  1:8338167/96‑4 (MQ=255)
                                                                                 cGTCAAGACGCCGCATTCGCGTAAGTGGCTGAGAATATGACGCACTGTGGTGCGGCTGATGTTGTACATTTCCGCCAGCGCACTTTGCGAT‑ggaag     <  2:13784202/96‑5 (MQ=255)
                                                                                   tCAAGACGCCGCATTCGCGTAAGTGGCTGAGAATATGACGCACTGTGGTGCGGCTGATGTTGTACATTTCCGCCAGCGCACTTTGCGATGGAAGTg    <  2:13999400/96‑1 (MQ=255)
                                                                                   tCAAGACGCCGCATTCGCGTAAGTGGCTGAGAATATGACGCACTGTGGTGCGGCTGATGTTGTACATTTCCGCCAGCGCACTTTGCGATGGAAGTg    <  1:13619345/96‑1 (MQ=255)
                                                                                   tCAAGACGCCGCATTCGCGTAAGTGGCTGAGAATATGACGCACTGTGGTGCGGCTGATGTTGTACATTTCCGCCAGCGCACTTTGCGATGGAAGTg    <  1:4503225/96‑1 (MQ=255)
                                                                                    cAAGACGCCGCATTCGCGTAAGTGGCTGAGAATATGACGCACTGTCGTGCGGCTGATGTTGTACATTTCCGCCAGCGCACTTTGCGATGGAAGTgg   >  2:10393294/1‑96 (MQ=255)
                                                                                     aaGACGCCGCATTCGCGTAAGTGGCTGAGAATATGACGCACTGTGGTGCGGCTGATGTTGTACATTTCCGCCAGCGCACTTTGCGATGGAAGTggg  >  1:4264411/1‑96 (MQ=255)
                                                                                     aaGACGCCGCATTCGCGTAAGTGGCTGAGAATATGACGCACTGTGGTGCGGCTGATGTTGTACATTTCCGCCAGCGCACTTTGCGATGGAAGTggg  <  2:9398209/96‑1 (MQ=255)
                                                                                     aaGACGCCGCATTCGCGTAAGTGGCTGAGAATATGACGCACTGTGGTGCGGCTGATGTTGTACATTTCCGCCAGCGCACTTTGCGATGGAAGTggg  >  2:2559150/1‑96 (MQ=255)
                                                                                          ||                                                                                         
TTCACTCATTGAAGCGGTAGTACAGGCGAAACCGTCATCATGGTCAGGTTTACGCGCAATAACATAGTCGTTTCCAACCTGCGTCAAGACCGCGCATTCGCGTAAGTGGCTGAGAATATGACGCACTGTGGTGCGGCTGATGTTGTACATTTCCGCCAGCGCACTTTGCGAT‑GAAGTGGG  >  NC_000913/4593164‑4593343

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 17 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output Only 200 of 285 total aligned reads displayed.
CTTTGTTTTGTTCACTCATTGAAGCGGTAGTACAGGCGAAACCGTCATCATGGTCAGGTTTACGCGCAATAACATAGTCGTTTCCAACCTGCGTCAAGACCGCGCATTCGCGTAAGTGGCTGAGAATATGACGCACTGTGGTGCGGCTGATGTTGTACATTTCCGCCAGCGCACTTTGCGAT‑G‑AAGTGGGGAAGGGATAT  >  NC_000913/4593154‑4593353
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CTTTGTTTTGTTCACTCATTGAAGCGGTAGTACAGGCGAAACCGTCATCATGGTCAGGTTTACGCGCAATAACATAGTCGTTTCCAACCTGCGTCAAGNNN                                                                                                       >  SRR4302518.8651104/1‑101 (MQ=60)
CTTTGTTTTGTTCACTCATTGAAGCGGTAGTACAGGCGAAACCGTCATCATGGTCAGGTTTACGCGCAATAACATAGTCGTTTCCAACCTGCGTCAAGACG                                                                                                       <  SRR4302518.2112696/101‑1 (MQ=60)
     TTTTGTTCACTCATTGAAGCGGTAGTACAGGAGAAACCGTCTTCATGGGCAGGTTTACGCGCAATAACATAGTCGGTTCCAACCTGCTTCAAGACGCCGCA                                                                                                  >  SRR4302518.6992636/1‑101 (MQ=60)
     NNNTGTTCACTCATTGAAGCGGTAGTACAGGCGAAACCGTCATCATGGTCAGGTTTACGCGCAATAACATAGTCGTTTCCAACCTGCGTCAAGACGCCGCA                                                                                                  <  SRR4302518.10353456/101‑1 (MQ=60)
     NNNTGTTCACTCATTGAAGCGGTAGTACAGGCGAAACCGTCATCATGGTCAGGTTTACGCGCAATAACATAGTCGTTTCCAACCTGCGTCAAGACGCCGCA                                                                                                  <  SRR4302518.7843273/101‑1 (MQ=60)
     NNNTGTTCACTCATTGAAGCGGTAGTACAGGCGAAACCGTCATCATGGTCAGGTTTACGCGCAATAACATAGTCGTTTCCAACCTGCGTCAAGACGCCGCA                                                                                                  <  SRR4302518.11436894/101‑1 (MQ=60)
      TTTGTTCACTCATTGAAGCGGTAGTACAGGCGAAACCGTCATCATGGTCAGGTTTACGCGCAATAACATAGTCGTTTCCAACCTGCGTCAAGACGCCGCAT                                                                                                 <  SRR4302518.2158943/101‑1 (MQ=60)
       TTGTTCACTCATTGAAGCGGTAGTACAGGCGAAACCGTCATCATGGTCAGGTTTACGCGCAATAACATAGTCGTTTCCAACCTGCGTCAAGACGCCGCATT                                                                                                >  SRR4302518.3837284/1‑101 (MQ=60)
       TTGTTCACTCATTGAAGCGGTAGTACAGGCGAAACCGTCATCATGGTCAGGTTTACGCGCAATAACATAGTCGTTTCCAACCTGCGTCAAGACGCCGCATT                                                                                                <  SRR4302518.7175091/101‑1 (MQ=60)
        TTTTCACTCATTGAAGCGGTAGTACAGGCGAAACCGTCATCATGGTCAGGTTTACGCGCAATAACATAGTCGTTTCCAACCTGCGTCAAGACGCCGCATTC                                                                                               <  SRR4302518.4657999/101‑1 (MQ=60)
        TGTTCACTCATTGAAGCGGTAGTACAGGCGAAACCGTCATCATGGTCAGGTTTACGCGCAATAACATAGTCGTTTCCAACCTGCGTCAAGACGCCGCATTC                                                                                               <  SRR4302518.605613/101‑1 (MQ=60)
        TGTTCACTCATTGAAGCGGTAGTACAGGCGAAACCGTCATCATGGTCAGGTTTACGCGCAATAACATAGTCGTTTCCAACCTGCGTCAAGACGCCGCATTC                                                                                               <  SRR4302518.3178382/101‑1 (MQ=60)
        TGTTCACTCATTGAAGCGGTAGTACAGGCGAAACCGTCATCATGGTCAGGTTTACGCGCAATAACATAGTCGTTTCCAACCTGCGTCAAGACGCCGCATTC                                                                                               <  SRR4302518.3917320/101‑1 (MQ=60)
        TGTTCACTCATTGAAGCGGTAATACAGGCGAAACCGTCATCATGGTCAGGTTTACGCGCAATAACATAGTCGTTTCCAACCTGCGTCAAGACGCCGCATTC                                                                                               <  SRR4302518.270153/101‑1 (MQ=60)
        NNNTCACTCATTGAAGCGGTAGTACAGGCGAAACCGTCATCATGGTCAGGTTTACGCGCAATAACATAGTCGTTTCCAACCTGCGTCAAGACGCCGCATTC                                                                                               <  SRR4302518.10896711/101‑1 (MQ=60)
         TTTCACTCATTGAAGCGGTAGTACAGGCGAAACCGTCATCATGGTCAGGTTTACGCGCAATAACATAGTCGTTTCCAACCTGCGTCAAGACGCCGCATTCG                                                                                              <  SRR4302518.3936476/101‑1 (MQ=60)
          TTCACTCATTGAAGCGGTAGTACAGGCGAAACCGTCATCATGGTCAGGTTTACGCGCAATAACATAGTCGTTTCCAACCTGCGTCAAGACGCCGCATTCGC                                                                                             <  SRR4302518.2236876/101‑1 (MQ=60)
          TTCACTCATTGAAGCGGTAGTACAGGCGAAACCGTCATCATGGTCAGGTTTACGCGCAATAACATAGTCGTTTCCAACCTGCGTCAAGACGCCGCATTCGC                                                                                             <  SRR4302518.7306941/101‑1 (MQ=60)
           TCACTCATTGAAGCGGTAGTACAGGCGAAACCGTCATCATGGTCAGGTTTACGCGCAATAACATAGTCGTTTCCAACCTGCGTCAAGACGCCGCATTCGCG                                                                                            >  SRR4302518.4308693/1‑101 (MQ=60)
           NNNCTCATTGAAGCGGTAGTACAGGCGAAACCGTCATCATGGTCAGGTTTACGCGCAATAACATAGTCGTTTCCAACCTGCGTCAAGACGCCGCATTCGCG                                                                                            <  SRR4302518.14159456/101‑1 (MQ=60)
            tgCTCATTGCAGCGGTAGTACAGGCGAAACCGTCATCATGGTCAGGTTTACGCGCCATAACATAGTCGTTTCCAACCTGCGTCAAGACGCCGCATTCGCGC                                                                                           <  SRR4302518.4860240/99‑1 (MQ=60)
            CACTCATTGAAGCGGTAGTACAGGCGAAACCGTCATCATGGTCAGGTTTACGCGCAATAACATAGTCGTTTCCAACCTGCGTCAAGACGCCGCATTCGCGT                                                                                           <  SRR4302518.3882368/101‑1 (MQ=60)
            CACTCATTGAAGCGGTAGTACAGGCGAAACCGTCATCATGGTCAGGTTTACGCGCAATAACATAGTCGTTTCCAACCTGCGTCAAGACGCCGCATTCGCGT                                                                                           <  SRR4302518.3847409/101‑1 (MQ=60)
            CACTCATTGAAGCGGTAGTACAGGCGAAACCGTCATCATGGTCAGGTTTACGCGCAATAACATAGTCGTTTCCAACCTGCGTCAAGACGCCGCATTCGCGT                                                                                           <  SRR4302518.2523327/101‑1 (MQ=60)
            CACTCATGGAAGCGGTAGTACAGGCGAAACCGTCATCATGGTCAGGTTTACGCGCAATAACATAGTCGTTTCCAACCTGCGTCAAGACGCCGCATTCGCGT                                                                                           <  SRR4302518.1265212/101‑1 (MQ=60)
             NNNCATTGAAGCGGTAGTACAGGCGAAACCGTCATCAAGGTCAGGTTTACGCGCAATAACATAGTCGTTTCCAACCTGCGTCAAGACGCCGCATTCGCGTA                                                                                          <  SRR4302518.12891207/101‑1 (MQ=60)
             ACTCATTGAAGCGGTAGTACAGGCGAAACCGTCATCATGGTCAGGTTTACGCGCAATAACATAGTCGTTTCCAACCTGCGTCAAGACGCCGCATTCGCNNN                                                                                          >  SRR4302518.8112760/1‑101 (MQ=60)
             ACTCATTGAAGCGGTAGTACAGGCGAAACCGTCATCATGGTCAGGTTTACGCGCAATAACATAGTCGTTTCCAACCTGCGTCAAGACGCCGCATTCGCGTA                                                                                          <  SRR4302518.642229/101‑1 (MQ=60)
             ACTCATTGAAGCGGTAGTACAGGCGAAACCGTCATCATGGTCAGGTTTACGCGCAATAACATAGTCGTTTCCAACCTGCGTCAAGACGCCGCATTCGCGTA                                                                                          <  SRR4302518.5102994/101‑1 (MQ=60)
              CTCATTGAAGCGGTAGTACAGGCGAAACCGTCATCATGGTCAGGTTTACGCGCAATAACATAGTCGTTTCCAACCTGCGTCAAGACGCCGCATTCGCGTAA                                                                                         >  SRR4302518.4719544/1‑101 (MQ=60)
              CTCATTGAAGCGGTAGTACAGGCGAAACCGTCATCATGGTCAGGTTTACGCGCAATAACATAGTCGTTTCCAACCTGCGTCAAGACGCCGCATTCGCGTAA                                                                                         <  SRR4302518.5391444/101‑1 (MQ=60)
               TCATTGAAGCGGTAGTACAGGCGAAACCGTCATCATGGTCAGGTTTACGCGCAATAACATAGTCGTTTCCAACCTGCGTCAAGACGCCGCATTCGCGTNNN                                                                                        >  SRR4302518.8736839/1‑101 (MQ=60)
                CATTGAAGCGGTAGTACAGGCGAAACCGTCATCATGGTCAGGTTTACGCGCAATAACATAGTCGTTTCCAACCTGCGTCAAGACGCCGCATTCGCGTAAGT                                                                                       <  SRR4302518.4925663/101‑1 (MQ=60)
                 ATTGAAGCGGTAGTACAGGCGAAACCGTCATCATGGTCAGGTTTACGCGCAATAACATAGTCGTTTCCAACCTGCGTCAAGACGCCGCATTCGCGTAANNN                                                                                      >  SRR4302518.8149821/1‑101 (MQ=60)
                 ATTGAAGCGGTAGTACAGGCGAAACCGTCATCATGGTCAGGTTTACGCGCAATAACATAGTCGTTTCCAACCTGCGTCAAGACGCCGCATTCGCGTAAGTG                                                                                      <  SRR4302518.3598094/101‑1 (MQ=60)
                 ATTGAAGCGGTAGTACAGGCGAAACCGTCATCATGGTCAGGTTTACGCGCAATAACATAGTCGTTTCCAACCTGCGTCAAGACGCCGCATTCGCGTAAGTG                                                                                      >  SRR4302518.6955950/1‑101 (MQ=60)
                 ATTGAAGCGGTAGTACAGGCGAAACCGTCATCATGGTCAGGTTTACGCGCAATAACATAGTCGTTTCCAACCTGCGTCAAGACGCCGCATTCGCGTAAGTG                                                                                      >  SRR4302518.6974694/1‑101 (MQ=60)
                 ACTGAAGCGGTAGTACAGGGGAAACCGTCATCATGGTCAGGTTTACGCGCAATAACATAGTCGTTTCCAACCTGCGTCAAGACGCCGCATTAGCGTAANNN                                                                                      >  SRR4302518.11143770/1‑101 (MQ=60)
                  NNNAAGCGGTAGTACAGGCGAAACCGTCATCATGGTCAGGTTTACGCGCAATAACATAGTCGTTTCCAACCTGCGTCAAGACGCCGCATTCGCGTAAGTGG                                                                                     <  SRR4302518.7701825/101‑1 (MQ=60)
                   TGAAGCGGTAGTACAGGCGAAACCGTCATCATGGTCAGGTTTACGCGCAATAACATAGTCGTTTCCAACCTGCGTCAAGACGCCGCATTCGCGTAAGTNNN                                                                                    >  SRR4302518.13555873/1‑101 (MQ=60)
                   TGAAGCGGTAGTACAGGCGAAACCGTCATCATGGTCAGGTTTACGCGCAATAACATAGTCGTTTCCAACCTGCGTCAAGACGCCGCATTCGCGTAAGTGGC                                                                                    >  SRR4302518.6236357/1‑101 (MQ=60)
                    GAAGCGGTAGTACAGGCGAAACCGTCATCATGGTCAGGTTTACGCGCAATAACATAGTCGTTTCCAACCTGCGTCAAGACGCCGCATTCGCGTAAGTGGCT                                                                                   <  SRR4302518.2205528/101‑1 (MQ=60)
                     AAGCGGTAGTACAGGCGAAACCGTCATCATGGTCAGGTTTACGCGCAATAACATAGTCGGTTCCAACCTGCGTCAAGACGCCGCATTCGCGTAAGTGGCTG                                                                                  >  SRR4302518.5897139/1‑101 (MQ=60)
                      NNNGGTAGTACAGGCGAAACCGTCATCATGGTCAGGTTTACGCGCAATAACATAGTCGTTTCCAACCTGCGTCAAGACGCCGCATTCGCGTAAGTGGCTGA                                                                                 <  SRR4302518.9940080/101‑1 (MQ=60)
                      AGCGGTAGTACAGGCGAAACCGTCATCATGGTCAGGTTTACGCGCAATAACATAGTCGTTTCCAACCTGCGTCAAGACGCCGCATTCGCGTAAGTGGCNNN                                                                                 >  SRR4302518.10876461/1‑101 (MQ=60)
                      AGCGGTAGTACAGGCGAAACCGGCATCATGGTCAGGTTTACGCGCAATAACATAGTCGTTTCCAACCTGCGTCAAGACGCCGCATTCGCGTAAGTGGCTGA                                                                                 >  SRR4302518.4354279/1‑101 (MQ=60)
                        CGGTAGTACAGGCGAAACCGTCATCATGGTCAGGTTTACGCGCAATAACATAGTCGTTTCCAACCTGCGTCAAGACGCCGCATTCGCGTAAGTGGCTGNNN                                                                               >  SRR4302518.7849206/1‑101 (MQ=60)
                          NNNGTACAGGCGAAACCGTCATCATGGTCAGGTTTACGCGCAATAACATAGTCGTTTCCAACCTGCGTCAAGACGCCGCATTCGCGTAAGTGGCTGAGAAT                                                                             <  SRR4302518.10201902/101‑1 (MQ=60)
                          GGAGTTCAGGCGAAACCGTCATCATGGTCAGGTTTACGCGCAATAACATAGTCGTTTCCAACCTGCGTCAAGACGCCGCATTCGCGTAAGTGGCTGAGAAT                                                                             <  SRR4302518.3674591/101‑1 (MQ=60)
                            AGTACAGGCGAAACCGTCATCATGGTCAGGTTTACGCGCAATAACATAGTCGTTTCCAACCTGCGTCAAGACGCCGCATTCGCGTAAGTGGCTGAGAATAT                                                                           <  SRR4302518.1269901/101‑1 (MQ=60)
                            AGTACAGGAGAAACCGTCATCATGGGCAGGTTTACGCGCAATAACATAGTCGTTTCCAACCTGCGTCAAGACGCCGCATTCGCGTATGATGCTGAGAATAT                                                                           >  SRR4302518.6091815/1‑101 (MQ=60)
                             GTACAGGCGAAACCGTCATCATGGTCAGGTTTACGCGCAATAACATAGTCGTTTCCAACCTGCGTCAAGACGCCGCATTCGCGGAAGTGGCTGAGAATATG                                                                          >  SRR4302518.2546024/1‑101 (MQ=60)
                              TACAGGCGAAACCGTCATCATGGTCAGGTTTACGCGCAATAACATAGTCGTTTCCAACCTGCGTCAAGACGCCGCATTCGCGTAAGTGGCTGAGAATANNN                                                                         >  SRR4302518.13518650/1‑101 (MQ=60)
                               NNNGGCGAAACCGTCATCATGGTCAGGTTTACGCGCAATAACATAGTCGTTTCCAACCTGCGTCAAGACGCCGCATTCGCGTAAGTGGCTGAGAATATGAC                                                                        <  SRR4302518.15061382/101‑1 (MQ=60)
                               NNNGGCGAAACCGTCATCATGGTCAGGTTTACGCGCAATAACATAGTCGTTTCCAACCTGCGTCAAGACGCCGCATTCGCGTAAGTGGCTGAGAATATGAC                                                                        <  SRR4302518.9278860/101‑1 (MQ=60)
                               ACAGGCGAAACCGTCATCATGGTCGGGTTTACGCGCAATAACATAGTCGTTTCCAACCTGCGTCAAGACGCCGCATTCGCGGAAGTGGCTGAGAATATGAC                                                                        >  SRR4302518.714174/1‑101 (MQ=60)
                               ACAGGCGAAACCGTCATCATGGTCAGGTTTACGCGCAATAACATAGTCGTTTCCAACCTGCGTCAAGACGCCGCATTCGCGTAAGTGGCTGAGAATATGAC                                                                        >  SRR4302518.611499/1‑101 (MQ=60)
                                CAGGCGAAACCGTCATCATGGTCAGGTTTACGCGCAATAACATAGTCGTTTCCAACCTGCGTCAAGACGCCGCATTCGCGTAAGTGGCTGAGAATATGNNN                                                                       >  SRR4302518.12178042/1‑101 (MQ=60)
                                CAGGCGAAACCGTCATCATGGTCAGGTTTACGCGCAATAACATAGTCGTTTCCAACCTGCGTCAAGACGCCGCATTCGCGTAAGTGGCTGAGAATATGNNN                                                                       >  SRR4302518.9138261/1‑101 (MQ=60)
                                CAGGCGAAACCGTCATCATGGTCAGGTTTACGCGCAATAACATAGTCGTTTCCAACCTGCGTCAAGACGCCGCATTCGCGTAAGTGGCTGAGAATATGACG                                                                       <  SRR4302518.4463693/101‑1 (MQ=60)
                                 AGGCGAAACCGTCATCATGGTCAGGTTTACGCGCAATAACATAGTCGTTTCCAACCTGCGTCAAGACGCCGCATTCGCGTAAGTGGCTGAGAATATGACGC                                                                      >  SRR4302518.2737790/1‑101 (MQ=60)
                                 AGGCGAAACCGTCATCATGGTCAGGTTTACGCGCAATAACATAGTCGTTTCCAACCTGCGTCAAGACGCCGCATTCGCGTAAGTGGCTGAGAATAGGACGC                                                                      >  SRR4302518.5583836/1‑101 (MQ=60)
                                 AGGAGAAACCGTCATCATGGTCAGGGTTACGCGCAATAACATAGTCGTTTCCAACCTGCGTCAAGACGCCGCATTCGCGTAAGTGGCTGCGAATATGACGC                                                                      >  SRR4302518.4938006/1‑101 (MQ=60)
                                   NNNAAACCGTCATCATGGTCAGGTTTACGCGCAATAACATAGTCGTTTCCAACCTGCGTCAAGACGCCGCATTCGCGTAAGTGGCTGAGAATATGACGCAC                                                                    <  SRR4302518.10936935/101‑1 (MQ=60)
                                   GTGAAACCGTCATCATGGTCAGGTTTACGCGCAATAACATAGTCATTTCCAACCTGCGTCAAGACGCCGCATTCGCGTAAGTGGCTGAGAATATGACGNNN                                                                    >  SRR4302518.13649194/1‑101 (MQ=60)
                                   GCGAAACCGTCATCATGGTCAGGTTTACGCGCAATAACATAGTCGTTTCCAACCTGCGTCAAGACGCCGCATTCGCGTAAGTGGCTGAGAATATGACGCAC                                                                    >  SRR4302518.3576626/1‑101 (MQ=60)
                                    NNNAACCGTCATCATGGTCAGGTTTACGCGCAATAACATAGTCGTTTCCAACCTGCGTCAAGACGCCGCATTCGCGTAAGTGGCTGAGAATATGACGCACT                                                                   <  SRR4302518.11282749/101‑1 (MQ=60)
                                    CGAAACCGTCATCATGGTCAGGTTTACGCGCAATAACATAGTCGTTTCCAACCTGCGTCAAGACGCCGCATTCGCGTAAGTGGCTGAGAATATGACGCNNN                                                                   >  SRR4302518.9052615/1‑101 (MQ=60)
                                     GAAACCGTCATCATGGTCAGGTTTACGCGCAATAACATAGTCGTTTCCAACCTGCGTCAAGACGCCGCATTCGCGTAAGTGGCTGAGAATATGACGCANNN                                                                  >  SRR4302518.13162778/1‑101 (MQ=60)
                                     GAAACCGTCATCATGGTCAGGTTTACGCGCAATAACATAGTCGTTTCCAACCTGCGTCAAGACGCCGCATTCGCGTAAGTGGCTGAGAATATGACGCACTG                                                                  <  SRR4302518.4204242/101‑1 (MQ=60)
                                      AAACCGTCATCATGGTCAGGTTTACGCGCAATAACATAGTCGTTTCCAACCTGCGTCAAGCCGCCGCATTCGCGTAAGTGGCTGAGAATATGACGCACTGT                                                                 <  SRR4302518.2940704/101‑1 (MQ=60)
                                      AAACCGTCATCATGGTCAGGTTTACGCGCAATAACATAGTCGTTTCCAACCTGCGTCAAGACGCCGCATTCGCGTAAGTGGCTGAGAATATGACGCACTGT                                                                 <  SRR4302518.2932853/101‑1 (MQ=60)
                                      AAACCGTCATCATGGTCAGGTTTACGCGCAATAACATAGTCGTTTCCAACCTGCGTCAAGACGCCGCATTCGCGTAAGTGGCTGAGAATATGACGCACNNN                                                                 >  SRR4302518.8301112/1‑101 (MQ=60)
                                       NNNCGTCATCATGGTCAGGTTTACGCGCAATAACATAGTCGTTTCCAACCTGCGTCAAGACGCCGCATTCGCGTAAGTGGCTGAGAATATGACGCACTGTG                                                                <  SRR4302518.9398683/101‑1 (MQ=60)
                                       AACCGTCATCATGGTCAGGTTTACGCGCAATAACATAGTCGTTTCCAACCTGCGTCAAGACGCCGCATTCGCGTAAGTGGCTGAGAATATGACGCACTNNN                                                                >  SRR4302518.14358331/1‑101 (MQ=60)
                                       AACCGTCATCATGGTCAGGTTTACGCGCAATAACATAGTCGTTTCCAACCTGCGTCAAGACGCCGCATTCGCGTAAGTGGCTGAGAATATGACGCACTGTG                                                                <  SRR4302518.2919726/101‑1 (MQ=60)
                                       AACCGTCATCATGGTCAGGTTTACGCGCAATAACATAGTCGTTTCCAACCTGCGTCAAGACGCCGCATTCGCGTAAGTGGCTGAGAATATGACGCACTGTG                                                                <  SRR4302518.6198897/101‑1 (MQ=60)
                                       AACCGTCATCATGCTCAGGTTTACGCGCAATAACATAGTCGTTTCCAACCTGCGTCAAGACGCCGCATTCGCGTAAGTGGCTGAGAATATGACGCACTNNN                                                                >  SRR4302518.14059432/1‑101 (MQ=60)
                                        ACCGTCATCATGGTCAGGTTTACGCGCAATAACATAGTCGTTTCCAACCTGCGTCAAGAGGCGGCATTCGCGTAAGTGGCTGAGAATATGACGCACTGNNN                                                               >  SRR4302518.12091803/1‑101 (MQ=60)
                                        ACCGTCATCATGGTCAGGTTTACGCGCAATAACATAGTCGTTTCCAACCTGCGTCAAGACGCCGCATTCGCGTAAGTGGCTGAGAATATGACGCACTGTGG                                                               <  SRR4302518.6714849/101‑1 (MQ=60)
                                         NNNTCATCATGGTCAGGTTTACGCGCAATAACATAGTCGTTTCCAACCTGCGTCAAGACGCCGCATTCGCGTAAGTGGCTGAGAATATGACGCACTGTGGT                                                              <  SRR4302518.12103966/101‑1 (MQ=60)
                                         CCGTCATCATGGTCAGGTTTACGCGCAATAACATAGTCGTTTCCACCCTGCGTCAAGACGCCGCATCCGCGTAAGTGGCTGAGAATATGACGCACTGTGGT                                                              <  SRR4302518.805937/101‑1 (MQ=60)
                                          CGTCATAATGGTCAGGTTTACGCGCAATAACATAGTCGTTTCCAACCTGCGTCAAGACGCCGCATTCGCGTAAGTGGCTGAGAATATGACGCACTGTGGTG                                                             <  SRR4302518.4525513/101‑1 (MQ=60)
                                           NNNATCATGGTCAGGTTTACGCGCAATAACATAGTCGTTTCCAACCTGCGTCAAGACGCCGCATTCGCGTAAGTGGCTGAGAATATGACGCACTGTGGTGC                                                            <  SRR4302518.10207892/101‑1 (MQ=60)
                                           GTCATCATGGTCAGGTTTACGCGCAATAACATAGTCGTTTCCAACCTGCGTCAAGACGCCGCATTCGCGTAAGTGGCTGAGAATATGACGCACTGTGGTGC                                                            >  SRR4302518.658955/1‑101 (MQ=60)
                                           GTCATCATGGTCAGGTTTACGCGCAATAACATAGTCGTTTCCAACCTGCGTCAAGACGCCGCATTCGCGTAAGTGGCTGAGAATATGACGCACTGTGGNNN                                                            >  SRR4302518.9592278/1‑101 (MQ=60)
                                           GTCATCATGGTCAGGTTTACGCGCAATAACATAGTCGTTTCCAACCTGCGTCAAGACGCCGCATTCGCGTAAGTGGCTGAGAATATGACGCACTGTGGNNN                                                            >  SRR4302518.10379976/1‑101 (MQ=60)
                                            TCATCATGGTCAGGTTTACGCGCAATAACATAGTCGTTTCCAACCTGCGTCAAGACGCCGCATTCGCGTAAGTGGCTGAGAATATGACGCACTGTGtggcg                                                           >  SRR4302518.5524687/1‑96 (MQ=60)
                                            TCATCATGGTCAGGTTTACGCGCAATAACATAGTCGTTTCCAACCTGCGTCAAGACGCCGCATTCGCGTAAGTGGCTGAGAATATGACGCACTGTGGTGCG                                                           <  SRR4302518.7200685/101‑1 (MQ=60)
                                            TCATCATGGTCAGGTTTACGCGCAATAACATAGTCGTTTCCAACCTGCGTCAAGACGCCGCATTCGCGTAAGTGGCTGAGAATATGACGCACTGTGGTGCG                                                           <  SRR4302518.3290020/101‑1 (MQ=60)
                                            TCATCATGGTCAGGTTTACGCGCAATAACATAGTCGTTTCCAACCTGCGTCAAGACGCCGCATTCGCGTAAGTGGCTGAGAATATGACGCACTGTGGTGCG                                                           >  SRR4302518.5702402/1‑101 (MQ=60)
                                            TCATCATGGTCAGGTTTACGCGCAATAACATAGTCGTTTCCAACCTGCGTCAAGACGCCGCATTCGCGTAAGTGGCTGAGAATATGACGCACTGTGGTGCG                                                           <  SRR4302518.100346/101‑1 (MQ=60)
                                            NNNTCATGGTCAGGTTTACGCGCAATAACATAGTCGTTTCCAACCTGCGTCAAGACGCCGCATTCGCGTAAGTGGCTGAGAATATGACGCACTGTGGTGAG                                                           <  SRR4302518.9819603/101‑1 (MQ=60)
                                             NNNCATGGTCAGGTTTACGCGCAATAACATAGTCGTTTCCAACCTGCGTCAAGACGCCGCATTCGCGTAAGTGGCTGAGAATATGACGCACTGTGGTGCGG                                                          <  SRR4302518.7563632/101‑1 (MQ=60)
                                             CATCATGGTCAGGTTTACGCGCAATAACATAGTCGTTTCCAACCTGCGTCAAGACGCCGCATTCGCGTAAGTGGCTGAGAATATGACGCACTGTGGTGCGG                                                          <  SRR4302518.120995/101‑1 (MQ=60)
                                             CATCATGGTCAGGTTTACGCGCAATAACATAGTCGTTTCCAACCTGCGTCAAGACGCCGCATTCGCGTAAGTGGCTGAGAATATGACGCACTGTGGTGCGG                                                          >  SRR4302518.2233216/1‑101 (MQ=60)
                                              TTCATGGTCAGGTTTACGCGCAATAACATAGTCGTTTCCAACCTGCGTCAAGACGCCGCATTCGAGTAAGTGGCTGATAATATGACGCACTGTGGTGTGGC                                                         >  SRR4302518.6762068/1‑101 (MQ=60)
                                              NNNATGGTCAGGTTTACGCGCAATAACATAGTCGTTTCCAACCTGCGTCAAGACGCCGCATTCGCGTAAGTGGCTGAGAATATGACGCACTGTGGTGCGGC                                                         <  SRR4302518.10413090/101‑1 (MQ=60)
                                              ATCATGGTCAGGTTTACGCGCAATAACATAGTCGTTTCCAACCTGCGTCAAGACGCCGCATTCGCGTAAGTGGCTGAGAATATGACGCACTGTGGTGCGGC                                                         >  SRR4302518.5699500/1‑101 (MQ=60)
                                               TCATGGTCAGGTTTACGCGCAATAACATAGTCGTTTCCAACCTGCGTCAAGACGCCGCATTCGCGTAAGTGGCTGAGAATATGACGCACTGTGGTGCGGCT                                                        <  SRR4302518.6665384/101‑1 (MQ=60)
                                               NNNTGGTCAGGTTTACGCGCAATAACATAGTCGTTTCCAACCTGCGTCAAGACGCCGCATTCGCGTAAGTGGCTGAGAATATGACGCACTGTGGTGCGGCT                                                        <  SRR4302518.7689538/101‑1 (MQ=60)
                                                CATGGTCAGGTTTACGCGCAATAACATAGTCGTTTCCAACCTGCGTCAAGACGCCGCATTCGCGTAAGGGGCTGAGAATATGACGCACTGTGGGGCGGCTG                                                       >  SRR4302518.390463/1‑101 (MQ=60)
                                                 nnnagCAGGTTTACGCGCAATAACATAGTCGTTTCCAACCTGCGTCAAGACGCCGCATTCGCGTAAGTGGCTGAGAATATGACGCACTGTGGTGCGGCTGA                                                      <  SRR4302518.7404293/96‑1 (MQ=60)
                                                 ATGGTCAGGTTTACGCGCAATAACATAGTCGTTTACAACCTGCGTCAAGACGACGCATTCGCGTAAGTGGCTGAGAATATGACGCAATGTGGTGCGGCAGA                                                      >  SRR4302518.6590893/1‑101 (MQ=60)
                                                  TGGTCAGGTTTACGCGCAATAACATAGTCGTTTCCAACCTGCGTCAAGACGCCGCATTCGCGTAAGTGGCTGAGAATATGACGCACTGTGGTGCGGCTGAT                                                     >  SRR4302518.4014863/1‑101 (MQ=60)
                                                  TGGTCAGGTTTACGCGCAATAACATAGTCGTTTCCAACCTGCGTCAAGACGCAGCATTCGCGTAAGAGGCTGAGAATATTGCGCACTGTGGTGCGGCTNNN                                                     >  SRR4302518.14576569/1‑101 (MQ=60)
                                                  NNNTCAGGTTTACGCGCAATAACATAGTCGTTTCCAACCTGCGTCAAGACGCCGCATTCGCGTAAGTGGCTGAGAATATGACGCACTGTGGTGCGGCTGAT                                                     <  SRR4302518.7463982/101‑1 (MQ=60)
                                                    GTCAGGTTTACGCGCAATAACATAGTCGTTTCCAACCTGCGTCAAGACGCCGCATTCGCGTAAGTGGCTGAGAATATGACGCACTGTGGTGCGGCTGATTT                                                   >  SRR4302518.3454037/1‑101 (MQ=60)
                                                    GTCAGGTTTACGCGCAATAACATAGTCGTTTCCAACCTGCGTCAAGACGCCGCATTCGCGGAAGTGGCTGAGAATATGACGCACTGTGGTGCGGCTGGTGT                                                   >  SRR4302518.1361198/1‑101 (MQ=60)
                                                     TCAGGTTTACGCGCAATAACATAGTCGTTTCCAACCTGCGTCAAGACGCCGCATTCGCGTAAGTGGCTGAGAATATGACGCACTGTGGTGCGGCTGATGTT                                                  >  SRR4302518.5824470/1‑101 (MQ=60)
                                                      NNNGTTTACGCGCAATAACATAGTCGTTTCCAACCTGCGTCAAGACGCCGCATTCGCGTAAGTGGCTGAGAATATGACGCACTGTGGTGCGGCTGATGTTG                                                 <  SRR4302518.12450483/101‑1 (MQ=60)
                                                       NNNTTTACGCGCAATAACATAGTCGTTTCCAACCCGCGTCAAGACGCCGCATTCCCGTAAGTGGCTGAGAATATGACGCACTGTGGTGCGGCTGATGTTAT                                                <  SRR4302518.12225821/101‑1 (MQ=60)
                                                       AGGTTTACGCGCAATAACATAGTCGTTTCCAACCTGCGTCAAGACGCCGCATTCGCGTAAGTGGCTGAGAATATGACGCACTGTGGTGCGGCTGGTGTTGG                                                >  SRR4302518.1004177/1‑101 (MQ=60)
                                                       AGGTTTACGCGCAATAACATAGTCGTTTCCAACCTGCGTCAAGACGCCGCATTCGCGTAAGTGGCTGAGAATATGACGCACTGTGGTGCGGCTGATGTTGT                                                <  SRR4302518.1522241/101‑1 (MQ=60)
                                                       AGGTTTACGCGCAATAACATAGTCGTTTCCAACCTGCGTCAAGACGCCGCATTCGCGTAAGTGGCTGAGAATATGACGCACTGTGGTGCGGCTGATGTNNN                                                >  SRR4302518.10482694/1‑101 (MQ=60)
                                                        GGTTTACGCGCAATAACATAGTCGTTTCCAACCTGCGTCAAGACGCCGCATTCGCGTAAGTGGCTGAGAATATGACGCACTGTGGTGCGGCTGATGTTGTA                                               <  SRR4302518.5757448/101‑1 (MQ=60)
                                                        GGTTTACGCGCAATAACATAGTCGTTTCCAACCTGCGTCAAGACGCCGCATTCGCGTAAGTGGCTGAGAATATGACGCACTGTGGTGCGGCTGATGTTGTA                                               <  SRR4302518.2792267/101‑1 (MQ=60)
                                                         NNNTACGCGCAATAACATAGTCGTTTCCAACCTGCGTCAAGACGCCGCATTCGCGTAAGTGGCTGAGAATATGACGCACTGTGGTGCGGCTGATGTTGTAC                                              <  SRR4302518.8598439/101‑1 (MQ=60)
                                                         GTTTACGCGCAATAACATAGTCGTTTCCAACCTGCGTCAAGACGCCGCATTTGCGTAAGTGGCTGAGAATATGACGCACTGTGGGGCGGCTGATGTTGNNN                                              >  SRR4302518.14428055/1‑101 (MQ=60)
                                                         GCTTACGCGCAATAACATAGTCGTTTCCAACCTGCGTCAAGACGCCGCATTCGCGTAAGTGGCTGAGAATATGACGCACTGTGGTGCGGCTGATGTTGNNN                                              >  SRR4302518.11338299/1‑101 (MQ=60)
                                                          TTTACGCGCAATAACATAGTCGTTTCCAACCTGCGTCAAGACGCCGCATTCGCGTAAGTGGCTGAGAATATGACGCACTGTGGTGCGGCTGATGTTGTACA                                             >  SRR4302518.6458614/1‑101 (MQ=60)
                                                           TTACGCGCAATAACATAGTCGTTTCCAACCTGCGTCAAGACGCCGCATTCGCGTAAGTGGCTGAGAATATGATGCACTGTGGTGCGGCTGATGTTGTANNN                                            >  SRR4302518.10557521/1‑101 (MQ=60)
                                                           TTACGCGCAATAACATAGTCGTTTCCAACCTGCGTCAAGACGCCGCATTCGCGTAAGTGGCTGAGAATATGACGCACTGTGGTGCGGCTGATGTAGTANNN                                            >  SRR4302518.10932373/1‑101 (MQ=60)
                                                           NNNCGCGCAATAACATAGTCGTTTCCAACCTGCGTCAAGACGCCGCATTCGCGTAAGTGGCTGAGAATATGACGCACTGTGGTGCGGCTGATGTTGTACAT                                            <  SRR4302518.9512227/101‑1 (MQ=60)
                                                             NNNCGCAATAACATAGTCGGTTCCAACTTGCGTCAAGACGCCGCATTCGCGTAAGTGGCTGAGAATATGACGCACTGTGGTGCGGCTGATGTTGTACATTT                                          <  SRR4302518.14615307/101‑1 (MQ=60)
                                                             ACGCGCAATAACATAGTCGTTTCCAACCTGCGTCAAGACGCCGCATTCGCGTAAGTGGCTGAGAATATGACGCACTGTGGTGCGGCTGATGTTGTACATTT                                          <  SRR4302518.4163879/101‑1 (MQ=60)
                                                              CGCGCAATAACATAGTCGTTTCCAACCTGCGTCAAGACGCCGCATTCGCGTAAGTGGCTGAGAATATGACGCACTGTGGTGCGGCTGATGTTGTACATTTC                                         <  SRR4302518.6953336/101‑1 (MQ=60)
                                                               NNNCAATAACATAGTCGTTTCCAACCTGCGTCAAGACGCCGCATTCGCGTAAGTGGCTGAGAATATGACGCACTGTGGTGCGGCTGATGTTGTACATTTCC                                        <  SRR4302518.12794205/101‑1 (MQ=60)
                                                               GCGCAATAACATAGTCGTTTCCAACCTGCGTCAAGACGCCGCATTCGCGTAAGTGGCTGAGAATATGACGCACTGTGGTGCGGCTGAGGTTGTACATTNNN                                        >  SRR4302518.14019194/1‑101 (MQ=60)
                                                                CGCAATAACATAGTCGTTTCCAACCTGCGTCAAGACGCCGCATTCGCGTAAGTGGCTGAGAATATGACGCACTGTGGTGCGGCTGATGTTGTACATTTNNN                                       >  SRR4302518.9505265/1‑101 (MQ=60)
                                                                CGCAATAACATAGTCGTTTCCAACCTGCGTCAAGACGCCGCATTCGCGTAAGTGGCTGAGAATATGACGCACTGTGGTGCGGCTGATGTTGTACATTTCCG                                       >  SRR4302518.6472535/1‑101 (MQ=60)
                                                                 GCAATAACATAGTCGTTTCCAACCTGCGTCAAGACGCCGCATTCGCGTAAGTGGCTGAGAATATGACGCACTGTGGTGCGGCTGATGTTGTACATTTCCGC                                      <  SRR4302518.484720/101‑1 (MQ=60)
                                                                  nnntagCATAGTCGTTTCCAACCTGCGTCAAGACGCCGCATTCGCGTAAGTGGCTGAGAATATGACGCACTGTGGTGCGGCTGATGTTGTACATTTCCGCC                                     <  SRR4302518.14589745/95‑1 (MQ=60)
                                                                  CAATAACATAGTCGTTTCCAACCTGCGTCAAGACGCCGCATTCGCGTAAGTGGCTGAGAATATGACGCACTGTGGTGCGGCTGATGTTGTACATTTCCGCC                                     <  SRR4302518.4513494/101‑1 (MQ=60)
                                                                   AATAACATAGTCGTTTCCAACCTGCGTCAGGACGCCGCATTCGCGTAAGTGGCTGAGAATATGACGCACTGTGGTGCGGCTGATGTTGTACATTTCCGNNN                                    >  SRR4302518.12647448/1‑101 (MQ=60)
                                                                    ATAACATAGTCGTTTCCAACCTGCGTCAAGACGCCGCATTCGCGTAAGTGGCTGAGAATATGACGCACTGTGGTGCGGCTGATGTTGTACATTTCCGCCAG                                   <  SRR4302518.5078388/101‑1 (MQ=60)
                                                                     TAACATAGTCGTTTCCAACCGGCGTCAAGACGCCGCATTCGCGTAAGTGGCTGAGAATATGACGCACTGTGGTGCGGCTGATTTTGTACATTTCCGCCAGC                                  >  SRR4302518.2847289/1‑101 (MQ=60)
                                                                     NNNCATAGTCGTTTCCAACCTGCGTCAAGACGCCGCATTCGCGTAAGTGGCTGAGAATATGACGCACTGTGGTGCGGCTGATGTTGTACATTTCCGCCAGC                                  <  SRR4302518.11754599/101‑1 (MQ=60)
                                                                      AACATAGTCGTTTCCAACCTGCGTCAAGACGCCGCATTCGCGTAAGTGGCTGAGAATATGACGCACTGTGGTGCGGCTGATGTTGTACATTTCCGCCAGCG                                 >  SRR4302518.2493825/1‑101 (MQ=60)
                                                                      AACATAGTCGTTTCCAACCTGCGTCAAGACGCCGCATTCGCGTAAGTGGCTGAGAATATGACGCACTGTGGTGCGGCTGATGTTGTACATTTCCGCCAGCG                                 >  SRR4302518.5193983/1‑101 (MQ=60)
                                                                       ACATAGTCGTTTCCAACCTGCGTCAAGACGCCGCATTCGCGTAAGTGGCTGAGAATATGACGCACTGTGGTGCGGCTGATGTTGTACATTTCCGCCAGCGC                                >  SRR4302518.5420289/1‑101 (MQ=60)
                                                                       ACATAGTCGTTTCCAACCTGCGTCAAGACGCCGCATTCGCGTAAGTGGCTGAGAATATGACGCACTGTGGTGCGGCTGATGTTGTACATTTCCGCCAGCGC                                <  SRR4302518.3595922/101‑1 (MQ=60)
                                                                       ACATAGTCGTCTCCAACCTGCGTCAAGACGACGAATTCGCGTAAGTGGCTGAGAATATGACGCACTGTGGTGCGGCTGATGTTGCACATTTCCGCCAGTGC                                <  SRR4302518.5284797/101‑1 (MQ=60)
                                                                        NNNAGTCGTTTCCAACCTGCGTCAAGACGCCGCATTCGCGTAAGTGGCTGAGAATATGACGCACTGTGGTGCGGCTGATGTTGTACATTTCCGCCAGCGCG                               <  SRR4302518.11902475/101‑1 (MQ=60)
                                                                        CATAGTCGTTTCCAACCTGCGTCAAGACGCCGCATTCGCGTAAGTGGCTGAGAATATGACGCACTGTGGTGCGGCTGATGTTGTACATTTCCGCCAGCGCA                               <  SRR4302518.5966935/101‑1 (MQ=60)
                                                                         ATAGTCGTTTCCAACCTGCGTCAAGACGCCGCATTCGCGTAAGTGGCTGAGAATATGACGCACTGTGGTGCGGCTGATGTTGTACATTTCCGCCAGCGNNN                              >  SRR4302518.7403091/1‑101 (MQ=60)
                                                                         ATAGTCGTTTCCAACCTGCGTCAAGACGCCGCATTCGCGTAAGTGGCTGAGAATATGACGCACTGTGGTGCGGCTGATGTTGTACATTTCCGCCAGCGCAC                              >  SRR4302518.5375484/1‑101 (MQ=60)
                                                                          TAGTCGTTTCCAACCTGCGTCAAGACGGCGCATTCGCGTAAGTGGCTGAGAATATGACGCACTGTGGTGCGGCTGATGTTGTACATTTCCGACAGCGCNNN                             >  SRR4302518.14716872/1‑101 (MQ=60)
                                                                          TAGTCGTTTCCAACCTGCGTCAAGACGCCGCATTCGCGTAAGTGGCTGAGAATATGACGCACTGTGGTGCGGCTGATGTTGTACATTTCCGCCAGCGCNNN                             >  SRR4302518.8931661/1‑101 (MQ=60)
                                                                          TAGTCGTTTCCAACCTGCGTCAAGACGCCGCATTCGCGTAAGTGGCTGAGAATATGACGCACTGTGGTGCGGCTGATGTTGTACATTTCCGCCAGCGCNNN                             >  SRR4302518.8311505/1‑101 (MQ=60)
                                                                          NNNTCGTTTCCAACCTGCGTCAAGACGCCGCATTCGCGTAAGTGGCTGAGAATATGACGCACTGTGGTGCGGCTGATGTTGTACATTTCCGCCAGCGCATT                             <  SRR4302518.12399205/101‑1 (MQ=60)
                                                                           NNNCGTTTCCAACCTGCGTCAAGACGCCGCATTCGCGTAAGTGGCTGAGAATATGACGCACTGTGGTGCGGCTGATGTTGTACATTTCCGCCAGCGCCCTT                            <  SRR4302518.10037684/101‑1 (MQ=60)
                                                                           AGTCGTTTCCAACCTGCGTCAAGACGCCGCATTCGCGTAAGTGGCTGAGAATATGACGCACTGTGGTGCGGCTGATGTTGTACATTTCCGCCAGCGCANNN                            >  SRR4302518.9832839/1‑101 (MQ=60)
                                                                            GTCGTTTCCAACCTGCGTCAAGACGCCGCATTCGCGTAAGTGGCTGAGAATATGACGCACTGTGGTGCGGCTGATGTTGTACATTTCCGCCAGCGCACTTT                           <  SRR4302518.1772966/101‑1 (MQ=60)
                                                                            GTCGTTTCCAACCTGCGTCAAGACGCCGCATTCGCGTAAGTGGCTGAGAATATGACGCACTGTGGTGCGCCTGATGTTGTACATTTCCGCCAGCGCACTTT                           <  SRR4302518.6634126/101‑1 (MQ=60)
                                                                             TCGTTTCCAACCTGCGTCAAGACGCCGCATTCGCGTAAGTGGCTGAGAATATGACGCACTGTGGTGCGGCTGATGTTGTACATTTCCGCCAGCGCACTTTG                          <  SRR4302518.5243292/101‑1 (MQ=60)
                                                                             TCGTTTCCAACCTGCGTCAAGACGCCGCATTCGCGTAAGTGGCTGAGAATATGACGCACTGTGGTGCGGCTGATGTTGTACATTTCCGCCAGCGCACTTTG                          <  SRR4302518.874122/101‑1 (MQ=60)
                                                                             NNNTTTCCAACCTGCGTCAAGACGCCGCATTCGCGTAAGTGGCTGAGAATATGACGCACTGTGGTGCGGCTGATGTTGTACATTTCCGCCAGCGCACTTTG                          <  SRR4302518.9946493/101‑1 (MQ=60)
                                                                              NNNTTCCAACCTGCGTCAAGACGCCGCATTCGCGTAAGTGGCTGAGAATATGACGCACTGTGGTGCGGCTGATGTTGTACATTTCCGCCAGCGCACTTTGC                         <  SRR4302518.15067618/101‑1 (MQ=60)
                                                                              NNNTTCCAACCTGCGTCAAGACGCCGCATTCGCGTAAGTGGCTGAGAATATGACGCACTGTGGTGCGGCTGATGTTGTACATTTCCGCCAGCGCACTTTGC                         <  SRR4302518.7506824/101‑1 (MQ=60)
                                                                              CGTTTCCAACCTGCGTCAAGACGCCGCATTCGCGTAAGTGGCTGAGAATATGACGCACTGTGGTGCGGCTGATGTTGTACATTTCCGCCAGCGCACTTTGC                         >  SRR4302518.3417707/1‑101 (MQ=60)
                                                                              CGTTTCCAACCTGCGTCAAGACGCCGCATTCGCGTAAGTGGCTGAGAATATGACGCACTGTGGTGCGGCTGATGTTGTACATTTCCGCCAGCGCACTTTGC                         >  SRR4302518.3000541/1‑101 (MQ=60)
                                                                              CGTTTCCAACCTGCGTCAAGACGCCGCATTCGCGGAAGTGGCTGAGAATATGACGCACTGTGGTGCGGCTGATGTTGTACATTTCCGCCAGCGCACTTNNN                         >  SRR4302518.14127995/1‑101 (MQ=60)
                                                                              CGTTTCCAACCTGCGTCAAGACGACGCATTCGCGTAAGTGGCTGAGAATATGACGCACTGTGGTGCGGCTGATGTTGTACATTTCCGCCAGCGCACTTTGC                         <  SRR4302518.5101510/101‑1 (MQ=60)
                                                                                TTTCCAACCTGCGTCAAGACGCCGCATTCGCGTAAGTGGCTGAGAATATGACGCACTGTGGTGCGGCTGATGTTGTACATTTCCGCCAGCGCACTTTGNNN                       >  SRR4302518.11566496/1‑101 (MQ=60)
                                                                                TTTCCAACCTGCGTCAAGACGCCGCATTCGCGTAAGTGGCTGAGAATATGACGCACTGTGGTGCGGCTGATGTTGTACATTTCCGCCAGCGCACTTTGCGA                       <  SRR4302518.4302684/101‑1 (MQ=60)
                                                                                TTTCCAACCTGCGTCAAGACGCCGCATTCGCGTAAGTGGCTGAGAATATGACGCACTGTGGTGCGGCTGATGTTGTACATTTCCGCCAGCGCACTTTGCGA                       >  SRR4302518.1768186/1‑101 (MQ=60)
                                                                                TTTCCAACCTGCGTCAAGACGCCGCATTCGCGTAAGTGGCTGAGAAGATGACGCACTGTGGTGCGGCTGATGTTGTACATTTCCGCCAGCGCACTTTGNNN                       >  SRR4302518.8652017/1‑101 (MQ=60)
                                                                                 nnncatCCTGCGTCAAGACGCCGCATTCGCGTAAGTGGCTGAGAATATGACGCACTGTGTTGCGGCTGATGTTGTACATTTCCGCCAGCGCACTTTGCGAT                      <  SRR4302518.13751303/95‑1 (MQ=60)
                                                                                 TTCCAACCTGCGTCAAGACGCCGCATTCGCGTAAGTGGCTGAGAATATGACGCACTGTGGTGCGGCTGATGTTGTACCTTTCCGCCAGCGCACTTTGCGAT                      >  SRR4302518.7067938/1‑101 (MQ=60)
                                                                                 NNNCAACCTGCGTCAAGACGCCGCATTCGCGTAAGTGGCTGAGAATATGACGCACTGTGGTGCGGCTGATGTTGTACATTTCCGCCAGCGCACTTTGCGAT                      <  SRR4302518.8983059/101‑1 (MQ=60)
                                                                                 NNNCAACCTGCGTCAAGACGCCGCATTCGCGTAAGTGGCTGAGAATATGACGCACTGTGGTGCGGCTGATGTTGTACATTTCCGCCAGCGCACTTTGCGAT                      <  SRR4302518.12180262/101‑1 (MQ=60)
                                                                                  NNNAACCTGCGTCAAGACGCCGCATTCGCGTAAGTGGCTGAGAATATGACGCACTGTGGTGCGGCTGATGTTGTACATTTCCGCCAGCGCACTTTGCGAT‑G                    <  SRR4302518.9278945/101‑1 (MQ=60)
                                                                                   CCAACCTGCGTCAAGACGCCGCATTCGCGTAAGTGGCTGAGAATATGACGCACTGTGGTGCGGCTGATGTTGTACATTTCCGCCAGCGCACTTTGCGAT‑G‑G                  >  SRR4302518.1144319/1‑101 (MQ=60)
                                                                                    CAACCTGCGTCAAGACGCCGCATTCGCGTAAGTGGCTGAGAATATGACGCACTGTGGTGCGGCTGATGTTGTACATTTCCGCCAGCGCACTTTGCGAT‑G‑GA                 >  SRR4302518.842612/1‑101 (MQ=60)
                                                                                    CAACCTGCGTCAAGACGCCGCATTCGCGTAAGTGGCTGAGAATATGACGCACTGTGGTGCGGCTGATGTTGTACATTTCCGCCAGCGCACTTTGCGAT‑G‑GA                 >  SRR4302518.7110968/1‑101 (MQ=60)
                                                                                     NNNCTGCGTCAAGACGCCGCATTCGCGTAAGTGGCTGAGAATATGACGCACTGTGGTGCGGCTGATGTTGTACATTTCCGCCAGCGCACTTTGCGAT‑Ggaa                 <  SRR4302518.11330173/101‑4 (MQ=60)
                                                                                     NNNCTGCGTCAAGACGCCGCATTCGCGTAAGTGGCTGAGAATATGACGCACTGTGGTGCGGCTGATGTTGTACATTTCCGCCAGCGCACTTTGCGAT‑Ggaa                 <  SRR4302518.14402212/101‑4 (MQ=60)
                                                                                      ACCTGCGTCAAGACGCCGCATTCGCGTAAGTGGCTGAGAATATGACGCACTGTGGTGCGGCTGATGTTGTACATTTCCGCCAGCGCACTTTGCGAT‑Ggnnn                >  SRR4302518.8913678/1‑97 (MQ=60)
                                                                                       NNNGCGTCAAGACGCCGCATTAGCGTAAGTGGCTGAGAATATGACGCACTGTGGTGCGGCTGATGTTGTACATTTCCGCCAGCGCACTTTGCGATGG‑AAGT               <  SRR4302518.10831494/101‑1 (MQ=60)
                                                                                        NNNCGTCAAGACGCCGCATTCGCGTAAGTGGCTGAGAATATGACGCACTGTGGTGCGGCTGATGTTGTACATTTCCGCCAGCGCACTTTGCGATGG‑AAGTG              <  SRR4302518.14243824/101‑1 (MQ=60)
                                                                                        CTGCGTCAAGACGCCGCATTCGCGTAAGTGGCTGAGAATATGACGCACTGTGGTGCGGCTGATGTTGTACATTTCCGCCAGCGCACTTTGCGATGG‑AAGTG              <  SRR4302518.4737348/101‑1 (MQ=60)
                                                                                         TGCGTCAAGACGCCGCATTCGCGTAAGTGGCTGAGAATATGACGCACTGTGGTGCGGCTGATGTTGTACATTTCCGCCAGCGCACTTTGCGATGG‑AAGTGG             >  SRR4302518.5274945/1‑101 (MQ=60)
                                                                                         TGCGTCAAGACGCCGCATTCGCGTAAGTGGCTGAGAATATGACGCACTGTGGTGCGGCTGATGTTGTACATTTCCGCCAGCGCACTTTGCGATGG‑AAGTGG             >  SRR4302518.2002265/1‑101 (MQ=60)
                                                                                          GCGTCAAGACGCCGCATTCGCGTAAGTGGCTGAGAATATGACGCACTGTGGTGCGGCTGATGTTGTACATTTCCGCCAGCGCACTTTGCGATGG‑AAGTGGG            <  SRR4302518.2540592/101‑1 (MQ=60)
                                                                                          GCGTCAAGACGCCGCATTCGCGTAAGTGGCTGAGAATATGACGCACTGTGGTGCGGCTGATGTTGTACATTTCCGCCAGCGCACTTTGCGATGG‑AAGTGGG            <  SRR4302518.2690464/101‑1 (MQ=60)
                                                                                          CCGTCAAGACGCCGCATTCGCGTAAGGGGCTCAGAATATGACGCTCTGTGGTGCGGCTGATGTTGTACATTTCCGCCAGCGCACTTTGCTAT‑GTAAGTGGG            <  SRR4302518.1564021/101‑1 (MQ=60)
                                                                                           AGTCAAGACGCCGCATTCGCGTAAGTGGCTGAGAATATGACGCACTGTGGTGCGGCTGATGTTGTACATTTCCGCCAGCGCACTTTGCGATGG‑AAGTGGGG           >  SRR4302518.2384925/1‑101 (MQ=60)
                                                                                            GTCAAGACGCCGCATTCGCGTAAGTGGCTGAGAATATGACGCACTGTGGTGCGGCTGATGTTGTACATTTCCGCCAGCGCACTTTGCGATGG‑AAGTGGNNN          >  SRR4302518.14135196/1‑101 (MQ=60)
                                                                                            GTCAAGACGCCGCATTCGCGTAAGTGGCTGAGAATATGACGCACTGTGGTGCGGCTGATGTTGTACATTTCCGCCAGCGCACTTTGCGATGG‑AAGTGGNNN          >  SRR4302518.9609845/1‑101 (MQ=60)
                                                                                            GTCAAGACGCCGCATTCGCGTAAGTGGCTGAGAATATGACGCACTGTGGTGCGGCTGATGTTGTACATTTCCGCCAGCGCACTTTGCGATGG‑AAGTGGNNN          >  SRR4302518.11399371/1‑101 (MQ=60)
                                                                                            GTCAAGACGCCGCATTCGCGTAAGTGGCTGAGAATATGACGCACTGTGGTGCGGCTGATGTTGTACATTTCCGCCAGCGCACTTTGCGATGG‑AAGTGGGGA          <  SRR4302518.4070845/101‑1 (MQ=60)
                                                                                             TCAAGACGCCGCATTCGCGTAAGTGGCTGAGAATATGACGCACTGTGGTGCGGCTGATGTTGTACATTTCCGCCAGCGCACTTTGCGATGG‑AAGTGGGNNN         >  SRR4302518.15021459/1‑101 (MQ=60)
                                                                                             TCAAGACGCCGCATTCGCGTAAGTGGCTGAGAATATGACGCACTGTGGTGCGGCTGATGTTGTACATTTCCGCCAGCGCACTTTGCGATGG‑AAGTGGGGAA         >  SRR4302518.2096543/1‑101 (MQ=60)
                                                                                             TCAAGACGCCGCATTCGCGTAAGTGGCTGAGAATATGACGCACTGGGGCGCGGCTGATGTTGTACATTTCCCCCAGGGCACTTTGCGATGG‑AAGTGGGGAA         >  SRR4302518.6753090/1‑101 (MQ=60)
                                                                                               AAGACGCCGCATTCGCGTAAGTGGCTGAGAATATGACGCACTGTGGTGCGGCTGATGTTGTACATTTCCGCCAGCGCACTTTGCGATGG‑AAGTGGGGANNN       >  SRR4302518.13977219/1‑101 (MQ=60)
                                                                                               AAGACGCCGCATTCGCGTAAGTGGCTGAGAATATGACGCACTGTGGTGCGGCTGATGTTGTACATTTCCGCCAGCGCACTTTGCGATGG‑AAGTGGGGAAGG       <  SRR4302518.2806420/101‑1 (MQ=60)
                                                                                               AAGACGCCGCATTCGCGTAAGTGGCTGAGAATATGACGCACTGTGGTGCGGCTGATGTTGTACATTTCCGCCAGCGCACTTTGCGATGG‑AAGTGGGGAAGG       >  SRR4302518.823877/1‑101 (MQ=60)
                                                                                               AAGACGCCGCATTCGCGTAAGTGGCTGAGAATATGACGCACTGTGGTGCGGCTGATGTTGTACATTTCCGCCAGCGCACTTTGCGATGG‑AAGTGGGGAAGG       >  SRR4302518.4474566/1‑101 (MQ=60)
                                                                                                    NNNGCATTCGCGTAAGTGGCTGAGAATATGACGCACTGTGGTGCGGCTGATGTTGTACATTTCCGCCAGCGCACTTTGCGATGG‑AAGTGGGGAAGGGATAT  <  SRR4302518.13186547/101‑1 (MQ=60)
                                                                                                    |                                                                                                     
CTTTGTTTTGTTCACTCATTGAAGCGGTAGTACAGGCGAAACCGTCATCATGGTCAGGTTTACGCGCAATAACATAGTCGTTTCCAACCTGCGTCAAGACCGCGCATTCGCGTAAGTGGCTGAGAATATGACGCACTGTGGTGCGGCTGATGTTGTACATTTCCGCCAGCGCACTTTGCGAT‑G‑AAGTGGGGAAGGGATAT  >  NC_000913/4593154‑4593353

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 2 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑