Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I2 R1 882 254.4 12957588 98.0% 12698436 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 97,700 A→G N205S (AAC→AGC)  murD → UDP‑N‑acetylmuramoylalanine‑D‑glutamate ligase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_00091397,7000AG100.0% 801.9 / NA 230N205S (AAC→AGC) murDUDP‑N‑acetylmuramoylalanine‑D‑glutamate ligase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (134/96);  total (134/96)

Only 100 of 242 total aligned reads displayed.

CGACCATTCTGAACGTGACTGAAGATCATATGGATCGCTATCCGTTTGGTTTACAACAGTATCGTGCAGCAAAACTGCGCATTTACGAAAACGCGAAAGTTTGCGTGGTTAATGCTGATGATGCCTTAACAATGCCGATTCGCGGTGCGGATGAACGCTGCGTCAGCTTTGGCGTCAACA  >  NC_000913/97610‑97789
                                                                                          |                                                                                         
cGACCATTCTGAACGTGACTGAAGATCATATGGATCGCTATCCGTTTGGTTTACAACAGTATCGTGCAGCAAAACTGCGCATTTACGAAAGcg                                                                                         <  2:559667/93‑1 (MQ=255)
 gACCATTCTGAACGTGACTGAAGATCATATGGATCGCTATCCGTTTGGTTTACAACAGTATCGTGCAGCAAAACTGCGCATTTACGAAAgcgc                                                                                        >  2:5203400/1‑93 (MQ=255)
 gACCATTCTGAACGTGACTGAAGATCATATGGATCGCTATCCGTTTGGTTTACAACAGTATCGTGCAGCAAAACTGCGCATTTACGAAAgcgc                                                                                        >  1:2156982/1‑93 (MQ=255)
  aCCATTCTGAACGTGACTGAAGATCATATGGATCGCTATCCGTTTGGTTTACAACAGTATCGTGCAGCAAAACTGCGCATTTACGAAAGcgcg                                                                                       >  1:276639/1‑93 (MQ=255)
  aCCATTCTGAACGTGACTGAAGATCATATGGATCGCTATCCGTTTGGTTTACAACAGTATCGTGCAGCAAAACTGCGCATTTACGAAAGcgcg                                                                                       >  2:2000444/1‑93 (MQ=255)
  aCCATTCTGAACGTGACTGAAGATCATATGGATCGCTATCCGTTTGGTTTACAACAGTATCGTGCAGCAAAACTGCGCATTTACGAAAGcgcg                                                                                       >  2:1910746/1‑93 (MQ=255)
  aCCATTCTGAACGTGACTGAAGATCATATGGATCGCTATCCGTTTGGTTTACAACAGTATCGTGCAGCAAAACTGCGCATTTACGAAAGcgcg                                                                                       >  1:2483467/1‑93 (MQ=255)
    cATTCTGAACGTGACTGAAGATCATATGGATCGCTATCCGTTTGGTTTACAACAGTATCGTGCAGCAAAACTGCGCATTTACGAAAGCGCGaa                                                                                     >  1:3688680/1‑93 (MQ=255)
     aTTCTGAACGAGACTGAAGATCATATGGATCGCTATCCGTTTGGTTTACAACAGTATCGTGCAGCAAAACTGCGCATTTACGAAAGCGCGaaa                                                                                    >  2:6255160/1‑93 (MQ=255)
      ttCTGAACGTGACTGAAGATCATATGGATCGCTATCCGTTTGGTTTACAACAGTATCGTGCAGCAAAACTGCGCATTTACGAAAGCGCGAAAg                                                                                   <  2:653557/93‑1 (MQ=255)
      ttCTGAACGTGACTGAAGATCATATGGATCGCTATCCGTTTGGTTTACAACAGTATCGTGCAGCAAAACTGCGCATTTACGAAAGCGCGAAAg                                                                                   >  2:4635448/1‑93 (MQ=255)
         tGAACGTGGCTGAAGATCATATGGATCGCTATCCGTTTGGTTTACAACAATATCGTGCAGCAAAACTGCGCATTTACGAAAGCGCGAAAGttt                                                                                >  2:2898940/1‑93 (MQ=255)
         tGAACGTGACTGAAGATCATATGGATCGCTATCCGTTTGGTTTACAACAGTATCGTGCAGCAAAACTGCGCATTTACGAAAGCGCGAAAGttt                                                                                >  2:2399621/1‑93 (MQ=255)
         tGAACGTGACTGAAGATCATATGGATCGCTATCCGTTTGGTTTACAACAGTATCGTGCAGCAAAACTGCGCATTTACGAAAGCGCGAAAGttt                                                                                <  1:4861051/93‑1 (MQ=255)
         tGAACGTGACTGAAGATCATATGGATCGCTATCCGTTTGGTTTACAACAGTATCGTGCAGCAAAACTGCGCATTTACGAAAGCGCGAAAGttt                                                                                >  1:966213/1‑93 (MQ=255)
         tGAACGTGACTGAAGATCATATGGATCGCTATCCGTTTGGTTTACAACAGTATCGTGCAGCAAAACTGCGCATTTACGAAAGCGCGAAAGttt                                                                                <  1:1145652/93‑1 (MQ=255)
         tGAACGTGACTGAAGATCATATGGATCGCTATCCGTTTGGTTTACAACAGTATCGTGCAGCAAAACTGCGCATTTACGAAAGCGCGAAAGttt                                                                                >  2:894249/1‑93 (MQ=255)
          gAACGTGACTGAAGATCATATGGATCGCTATCCGTTTGGTTTACAACAGTATCGTGCAGCAAAACTGCGCATTTACGAAAGCGCGAAAGTTTg                                                                               <  1:6091560/93‑1 (MQ=255)
          gAACGTGACTGAAGATCATATGGATCGCTATCCGTTTGGTTTACAACAGTATCGTGCAGCAAAACTGCGCATTTACGAAAGCGCGAAAGTTTg                                                                               <  1:1323862/93‑1 (MQ=255)
           aaCGTGACTGAAGATCATATGGATCGCTATCCGTTTGGTTTACAACAGTATCGTGCAGCAAAACTGCGCATTTACGAAAGCGCGAAAGTTTGc                                                                              <  2:1818173/93‑1 (MQ=255)
           aaCGTGACTGAAGATCATATGGATCGCTATCCGTTTGGTTTACAACAGTATCGTGCAGCAAAACTGCGCATTTACGAAAGCGCGAAAGTTTGc                                                                              >  2:4603452/1‑93 (MQ=255)
             cGTGACTGAAGATCATATGGATCGCTATCCGTTTGGTTTACGACAGTATCGTGCAGCAAAGCTGCGCATTTACGAAAGCGCGAAAGTTTGCGt                                                                            <  1:814953/93‑1 (MQ=255)
             cGTGACTGAAGATCATATGGATCGCTATCCGTTTGGTTTACAACAGTATCGTGCAGCAAAACTGCGCATTTACGAAAGCGCGAAAGTTTGCGt                                                                            >  1:1187457/1‑93 (MQ=255)
             cGTGACTGAAGATCATATGGATCGCTATCCGTTTGGTTTACAACAGTATCGTGCAGCAAAACTGCGCATTTACGAAAGCGCGAAAGTTTGCGt                                                                            <  1:3918992/93‑1 (MQ=255)
             cGTGACTGAAGATCATATGGATCGCTATCCGTTTGGTTTACAACAGTATCGTGCAGCAAAACTGCGCATTTACGAAAGCGCGAAAGTTTGCGt                                                                            <  1:46365/93‑1 (MQ=255)
             cGTGACTGAAGATCATATGGATCGCTATCCGTTTGGTTTACAACAGTATCGTGCAGCAAAACTGCGCATTTACGAAAGCGCGAAAGTTTGCGt                                                                            >  2:2135640/1‑93 (MQ=255)
                 aCTGAAGATCATATGGATCGCTATCCGTTTGGTTTACAACAGTATCGTGCAGCAAAACTGCGCATTTACGAAAGCGCGAAAGTTTGCGTGGtt                                                                        >  1:93130/1‑93 (MQ=255)
                  cTGAAGATCATATGGATCGCTATCCGTTTGGTTTACAACAGTATCGTGCAGCAAAACTGCGCATTTACGAAAGCGCGAAAGTTTGCGTGGTTa                                                                       >  2:1919595/1‑93 (MQ=255)
                    gAAGATCATATGGATCGCTATCCGTTTGGTTTACAACAGTATCGTGCAGCAAAACTGCGCATTTACGAAAGCGCGAAAGTTTGCGTGGTTAAt                                                                     <  1:1730930/93‑1 (MQ=255)
                    gAAGATCATATGGATCGCTATCCGTTTGGTTTACAACAGTATCGTGCAGCAAAACTGCGCATTTACGAAAGCGCGAAAGTTTGCGTGGTTAAt                                                                     <  2:3983878/93‑1 (MQ=255)
                        aTCATATGGATCGCTATCCGTTTGGTTTACAACAGTATCGTGCAGCAAAACTGCGCATTTACGAAAGCGCGAAAGTTTGCGTGGTTAATGCtg                                                                 >  1:3232754/1‑93 (MQ=255)
                        aTCATATGGATCGCTATCCGTTTGGTTTACAACAGTATCGTGCAGCAAAACTGCGCATTTACGAAAGCGCGAAAGTTTGCGTGGTTAATGCtg                                                                 <  1:4485034/93‑1 (MQ=255)
                          cATATGGATCGCTATCCGTTTGGTTTACAACAGTATCGTGCAGCAAAACTGCGCATTTACGAAAGCGCGAAAGTTTGCGTGGTTAATGCtgat                                                               <  1:1926261/93‑1 (MQ=255)
                          cATATGGATCGCTATCCGTTTGGTTTACAACAGTATCGTGCAGCAAAACTGCGCATTTACGAAAGCGCGAAAGTTTGCGTGGTTAATGCtgat                                                               >  1:5266855/1‑93 (MQ=255)
                           atatGGATCGCTATCCGTTTGGTTTACAACAGTATCGTGCAGCAAAACTGCGCATTTACGAAAGCGCGAAAGTTTGCGTGGTTAATGCtgatg                                                              >  2:3808853/1‑93 (MQ=255)
                           atatGGATCGCTATCCGTTTGGTTTACAACAGTATCGTGCAGCAAAACTGCGCATTTACGAAAGCGCGAAAGTTTGCGTGGTTAATGCtgatg                                                              >  1:3136732/1‑93 (MQ=255)
                            tatGGATCGCTATCCGTTTGGTTTACAACAGTATCGTGCAGCAAAACTGCGCATTTACGAAAGCGCGAAAGTTTGCGTGGTTAATGCtgatga                                                             <  1:1660533/93‑1 (MQ=255)
                            tatGGATCGCTATCCGTTTGGTTTACAACAGTATCGTGCAGCAAAACTGCGCATTTACGAAAGCGCGAAAGTTTGCGTGGTTAATGCtgatga                                                             <  1:3274140/93‑1 (MQ=255)
                                gATCGCTATCCGTTTGGTTTACAACAGTATCGTGCAGCAAAACTGCGCATTTACGAAAGCGCGAAAGTTTGCGTGGTTAATGCTGATGATGcc                                                         >  2:3744079/1‑93 (MQ=255)
                                gATCGCTATCCGTTTGGTTTACAACAGTATCGTGCAGCAAAACTGCGCATTTACGAAAGCGCGAAAGTTTGCGTGGTTAATGCTGATGATGcc                                                         >  1:1536857/1‑93 (MQ=255)
                                    gCTATCCGTTTGGTTTACAACAGTATCGTGCAGCAAAACTGCGCATTTACGAAAGCGCGAAAGTTTGCGTGGTTAATGCTGATGCTGCCTTaa                                                     >  1:4309323/1‑93 (MQ=255)
                                    gCTATCCGTTTGGTTTACAACAGTATCGTGCAGCAAAACTGCGCATTTACGAAAGCGCGAAAGTTTGCGTGGTTAATGCTGATGATGCCTTaa                                                     >  1:3691698/1‑93 (MQ=255)
                                    gCTATCCGTTTGGTTTACAACAGTATCGTGCAGCAAAACTGCGCATTTACGAAAGCGCGAAAGTTTGCGTGGTTAATGCTGATGATGCCTTaa                                                     >  2:180053/1‑93 (MQ=255)
                                    gCTATCCGTTTGGTTTACAACAGTATCGTGCAGCAAAACTGCGCATTTACGAAAGAGCGAAAGTTTGCGTGGTTAATGCTGATGATGCCTTaa                                                     >  1:2109138/1‑93 (MQ=255)
                                      tATCCGTTTGGTTTACAACAGTATCGTGCAGCAAAACTGCGCATTTACGAAAGCGCGAAAGTTTGCGTGGTTAATGCTGATGATGCCTTAACa                                                   >  2:5312022/1‑93 (MQ=255)
                                       aTCCGTTTGGTTTACAACAGTATCGTGCAGCAAAACTGCGCATTTACGAAAGCGCGAAAGTTTGCGTGGTTAATGCTGATGATGCCTTAACaa                                                  >  1:1845143/1‑93 (MQ=255)
                                       aTCCGTTTGGTTTACAACAGTATCGTGCAGCAAAACTGCGCATTTACGAAAGCGCGAAAGTTTGCGTGGTTAATGCTGATGATGCCTTAACaa                                                  <  2:5854489/93‑1 (MQ=255)
                                        tCCGTTTGGTTTACAACAGTATCGTGCAGCAAAACTGCGCATTTACGAAAGCGCGAAAGTTTGCGTGGTTAATGCTGATGATGCCTTAACAAt                                                 <  1:6378146/93‑1 (MQ=255)
                                        tCCGTTTGGTTTACAACAGTATCGTGCAGCAAAACTGCGCATTTACGAAAGCGCGAAAGTTTGCGTGGTTAATGCTGATGATGCCTTAACAAt                                                 >  1:5689814/1‑93 (MQ=255)
                                         ccGTTTGGTTTACAACAGTATCGTGCAGCAAAACTGCGCATTTACGAAAGCGCGAAAGTTTGCGTGGTTAATGCTGATGATGCCTTAACAATg                                                <  1:4982552/93‑1 (MQ=255)
                                            tttGGTTTACAACAGTATCGTGCAGCAAAACTGCGCATTTACGAAAGCGCGAAAGTTTGCGTGGTTAATGCTGATGATGCCTTAACAATGCCg                                             >  1:6059510/1‑93 (MQ=255)
                                            tttGGTTTACAACAGTATCGTGCAGCAAAACTGCGCATTTACGAAAGCGCGAAAGTTTGCGTGGTTAATGCTGATGATGCCTTAACAATGCCg                                             >  2:2801997/1‑93 (MQ=255)
                                            tttGGTTTACAACAGTATCGTGCAGCAAAACTGCGCATTTACGAAAGCGCGAAAGTTTGCGTGGTTAATGCTGATGATGCCTTAACAATGCCg                                             <  2:5146561/93‑1 (MQ=255)
                                             ttGGTTTACAACAGTATCGTGCAGCAAAACTGCGCATTTACGAAAGCGCGAAAGTTTGCGTGGTTAATGCTGATGATGCCTTAACAATGCCGa                                            <  1:3360021/93‑1 (MQ=255)
                                              tGGTTTACAACAGTATCGTGCAGCAAAACTGCGCATTTACGAAAGCGCGAAAGTTTGCGTGGTTAATGCTGATGATGCCTTAACAATGCCGAt                                           <  2:4740802/93‑1 (MQ=255)
                                              tGGTTTACAACAGTATCGTGCAGCAAAACTGCGCATTTACGAAAGCGCGAAAGTTTGCGTGGTTAATGCTGATGATGCCTTAACAATGCCGAt                                           <  2:346812/93‑1 (MQ=255)
                                                gTTTACAACAGTATCGTGCAGCAAAACTGCGCATTTACGAAAGCGCGAAAGTCTGCGTGGTTAATGCTGATGATGCCTTAACAATGCCGATTc                                         <  2:5262783/93‑1 (MQ=255)
                                                 tttACAACAGTATCGTGCAGCAAAACTGCGCATTTACGAAAGCGCGAAAGTTTGCGTGGTTAATGCTGATGATGCCTTAACAATGCCGATTcg                                        >  1:4622654/1‑93 (MQ=255)
                                                  ttACAACAGTATCGTGCAGCAAAACTGCGCATTTACGAAAGCGCGAAAGTTTGCGTGGTTAATGCTGATGATGCCTTAACAATGCCGATTcgc                                       >  1:4338807/1‑93 (MQ=255)
                                                  ttACAACAGTATCGTGCAGCAAAACTGCGCATTTACGAAAGCGCGAAAGTTTGCGTGGTTAATGCTGATGATGCCTTAACAATGCCGATTcgc                                       <  2:332051/93‑1 (MQ=255)
                                                   tACAACAGTATCGTGCAGCAAAACTGCGCATTTACGAAAGCGCGAAAGTTTGCGTGGTTAATGCTGATGATGCCTTAACAATGCCGATTcgcg                                      >  2:1728873/1‑93 (MQ=255)
                                                   tACAACAGTATCGTGCAGCAAAACTGCGCATTTACGAAAGCGCGAAAGTTTGCGTGGTTAATGCTGATGATGCCTTAACAATGCCGATTcgcg                                      >  1:5561367/1‑93 (MQ=255)
                                                     caacaGTATCGTGCAGCAAAACTGCGCATTTACGAAAGCGCGAAAGTTTGCGTGGTTAATGCTGATGATGCCTTAACAATGCCGATTCGCGGt                                    >  1:2806387/1‑93 (MQ=255)
                                                      aacaGTATCGTGCAGCAAAACTGCGCATTTACGAAAGCGCGAAAGTTTGCGTGGTTAATGCTGATGATGCCTTAACAATGCCGATTCGCGGTg                                   >  2:1398314/1‑93 (MQ=255)
                                                      aacaGTATCGTGCAGCAAAACTGCGCATTTACGAAAGCGCGAAAGTTTGCGTGGTTAATGCTGATGATGCCTTAACAATGCCGATTCGCGGTg                                   >  1:3963543/1‑93 (MQ=255)
                                                          gTATCGTGCAGCAAAACTGCGCATTTACGAAAGCGCGAAAGTTTGCGTGGTTAATGCTGATGATGCCTTAACAATGCCGATTCGCGGTGCGGa                               >  2:5490909/1‑93 (MQ=255)
                                                          gTATCGTGCAGCAAAACTGCGCATTTACGAAAGCGCGAAAGTTTGCGTGGTTAATGCTGATGATGCCTTAACAATGCCGATTCGCGGTGCGGa                               >  1:5986046/1‑93 (MQ=255)
                                                          gTATCGTGCAGCAAAACTGCGCATTTACGAAAGCGCGAAAGTTTGCGTGGTTAATGCTGATGATGCCTTAACAATGCCGATTCGCGGTGCGGa                               <  2:2281881/93‑1 (MQ=255)
                                                          gTATCGTGCAGCAAAACTGCGCATTTACGAAAGCGCGAAAGTTTGCGTGGTTAATGCTGATGATGCCTTAACAATGCCGATTCGCGGTGCGGa                               >  1:2272992/1‑93 (MQ=255)
                                                           tATCGTGCAGCAAAACTGCGCATTTACGAAAGCGCGAAAGTTTGCGTGGTTAATGCTGATGATGCCTTAACAATGCCGATTCGCGGTGCGGAt                              >  2:4917876/1‑93 (MQ=255)
                                                           tATCGTGCAGCAAAACTGCGCATTTACGAAAGCGCGAAAGTTTGCGTGGTTAATGCTGATGATGCCTTAACAATGCCGATTCGCGGTGCGGAt                              <  1:2386823/93‑1 (MQ=255)
                                                           tATCGTGCAGCAAAACTGCGCATTTACGAAAGCGCGAAAGTTTGCGTGGTTAATGCTGATGATGCCTTAACAATGCCGATTCGCGGTGCGGAt                              <  2:3334191/93‑1 (MQ=255)
                                                           tATCGTGCAGCAAAACTGCGCATTTACGAAAGCGCGAAAGTTTGCGTGGTTAATGCTGATGATGCCTTAACAATGCCGATTCGCGGTGCGGAt                              <  2:4295061/93‑1 (MQ=255)
                                                            aTCGTGCAGCAAAACTGCGCATTTACGAAAGCGCGAAAGTTTGCGTGGTTAATGCTGATGATGCCTTAACAATGCCGATTCGCGGTGCGGATg                             >  1:5188353/1‑93 (MQ=255)
                                                             tCGTGCAGCAAAACTGCGCATTTACGAAAGCGCGAAAGTTTGCGTGGTTAATGCTGATGATGCCTTAACAATGCCGATTCGCGGTGCGGATGa                            <  2:2730924/93‑1 (MQ=255)
                                                             tCGTGCAGCAAAACTGCGCATTTACGAAAGCGCGAAAGTTTGCGTGGTTAATGCTGATGATGCCTTAACAATGCCGATTCGCGGTGCGGATGa                            <  1:4067923/93‑1 (MQ=255)
                                                               gTGCAGCAAAACTGCGCATTTACGAAAGCGCGAAAGTTTGCGTGGTTAATGCTGATGATGCCTTAACACTGCCGATTCGCGGTGCGGATGAAc                          >  2:4344523/1‑93 (MQ=255)
                                                                tGCAGCAAAACTGCGCATTTACGAAAGCGCGAAAGTTTGCGTGGTTAATGCTGATGATGCCTTAACAATGCCGATTCGCGGTGCGGATGAACg                         <  2:1606790/93‑1 (MQ=255)
                                                                 gcagcaAAACTGCGCATTTACGAAAGCGCGAAAGTTTGCGTGGTTAATGCTGATGATGCCTTAACAATGCCGATTCGCGGTGCGGATGAACGc                        >  1:4250664/1‑93 (MQ=255)
                                                                   agcaAAACTGCGCATTTACGAAAGCGCGAAAGTTTGCGTGGTTAATGCTGATGATGCCTTAACAATGCCGATTCGCGGTGCGGATGAACGCTg                      >  2:6042597/1‑93 (MQ=255)
                                                                   agcaAAACTGCGCATTTACGAAAGCGCGAAAGTTTGCGTGGTTAATGCTGATGATGCCTTAACAATGCCGATTCGCGGTGCGGATGAACGCTg                      >  1:6151242/1‑93 (MQ=255)
                                                                    gcaAAACTGCGCATTTACGAAAGCGCGAAAGTTTGCGTGGTTAATGCTGATGATGCCTTAACAATGCCGATTCGCGGTGCGGATGAACGCTGc                     >  1:4522176/1‑93 (MQ=255)
                                                                    gcaAAACTGCGCATTTACGAAAGCGCGAAAGTTTGCGTGGTTAATGCTGATGATGCCTTAACAATGCCGATTCGCGGTGCGGATGAACGCTGc                     >  1:5441125/1‑93 (MQ=255)
                                                                      aaaaCTGCGCATTTACGAAAGCGCGAAAGTTTGCGTGGTTAATGCTGATGATGCCTTAACAATGCCGATTCGCGGTGCGGATGAACGCTGCGt                   >  1:4160054/1‑93 (MQ=255)
                                                                      aaaaCTGCGCATTTACGAAAGCGCGAAAGTTTGCGTGGTTAATGCTGATGATGCCTTAACAATGCCGATTCGCGGTGCGGATGAACGCTGCGt                   >  2:1013746/1‑93 (MQ=255)
                                                                      aaaaCTGCGCATTTACGAAAGCGCGAAAGTTTGCGTGGTTAATGCTGATGATGCCTTAACAATGCCGATTCGCGGTGCGGATGAACGCTGCGt                   >  1:3804541/1‑93 (MQ=255)
                                                                         aCTGCGCATTTACGAAAGCGCGAAAGTTTGCGTGGTTAATGCTGATGATGCCTTAACAATGCCGATTCGCGGTGCGGATGAACGCTGCGTCAg                >  2:5731931/1‑93 (MQ=255)
                                                                          cTGCGCATTTACGAAAGCGCGAAAGTTTGCGTGGTTAATGCTGATGATGCCTTAACAATGCCGATTCGCGGTGCGGATGAACGCTGCGTCAGc               >  2:2630949/1‑93 (MQ=255)
                                                                           tGCGCATTTACGAAAGCGCGAAAGTTTGCGTGGTTAATGCTGATGATGCCTTAACAATGCCGATTCGCGGTGCGGATGAACGCTGCGTCAGCt              >  2:4057777/1‑93 (MQ=255)
                                                                           tGCGCATTTACGAAAGCGCGAAAGTTTGCGTGGTTAATGCTGATGATGCCTTAACAATGCCGATTCGCGGTGCGGATGAACGCTGCGTCAGCt              >  2:5771951/1‑93 (MQ=255)
                                                                           tGCGCATTTACGAAAGCGCGAAAGTTTGCGTGGTTAATGCTGATGATGCCTTAACAATGCCGATTCGCGGTGCGGATGAACGCTGCGTCAGCt              >  1:3497272/1‑93 (MQ=255)
                                                                            gcgcATTTACGAAAGCGCGAAAGTTTGCGTGGTTAATGCTGATGATGCCTTAACAATGCCGATTCGCGGTGCGGATGAACGCTGCGTCAGCtt             <  2:4866264/93‑1 (MQ=255)
                                                                            gcgcATTTACGAAAGCGCGAAAGTTTGCGTGGTTAATGCTGATGATGCCTTAACAATGCCGATTCGCGGTGCGGATGAACGCTGCGTCAGCtt             >  1:110246/1‑93 (MQ=255)
                                                                               cATTTACGAAAGCGCGAAAGTTTGCGTGGTTAATGCTGATGATGCCTTAACAATGCCGATTCGCGGTGCGGATGAACGCTGCGTCAGCTTTgg          >  2:802641/1‑93 (MQ=255)
                                                                                  ttACGAAAGCGCGAAAGTTTGCGTGGTTAATGCTGATGATGCCTTAACAATGCCGATTCGCGGTGCGGATGAACGCTGCGTCAGCTTTGGCGt       >  1:1986245/1‑93 (MQ=255)
                                                                                     cGAAAGCGCGAAAGTTTGCGTGGTTAATGCTGATGATGCCTTAACAATGCCGATTCGCGGTGCGGATGAACGCTGCGTCAGCTTTGGCGTCaa    <  2:5324564/93‑1 (MQ=255)
                                                                                     cGAAAGCGCGAAAGTTTGCGTGGTTAATGCTGATGATGCCTTAACAATGCCGATTCGCGGTGCGGATGAACGCTGCGTCAGCTTTGGCGTCaa    >  2:2070656/1‑93 (MQ=255)
                                                                                       aaagCGCGAAAGTTTGCGTGGTTAATGCTGATGATGCCTTAACAATGCCGATTCGCGGTGCGGATGAACGCTGCGTCAGCTTTGGCGTCAACa  <  1:760021/93‑1 (MQ=255)
                                                                                       aaagCGCGAAAGTTTGCGTGGTTAATGCTGATGATGCCTTAACAATGCCGATTCGCGGTGCGGATGAACGCTGCGTCAGCTTTGGCGTCAACa  <  2:6115673/93‑1 (MQ=255)
                                                                                       aaagCGCGAAAGTTTGCGTGGTTAATGCTGATGATGCCTTAACAATGCCGATTCGCGGTGCGGATGAACGCTGCGTCAGCTTTGGCGTCAACa  >  2:3151438/1‑93 (MQ=255)
                                                                                          |                                                                                         
CGACCATTCTGAACGTGACTGAAGATCATATGGATCGCTATCCGTTTGGTTTACAACAGTATCGTGCAGCAAAACTGCGCATTTACGAAAACGCGAAAGTTTGCGTGGTTAATGCTGATGATGCCTTAACAATGCCGATTCGCGGTGCGGATGAACGCTGCGTCAGCTTTGGCGTCAACA  >  NC_000913/97610‑97789

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
CAGCGACCATTCTGAACGTGACTGAAGATCATATGGATCGCTATCCGTTTGGTTTACAACAGTATCGTGCAGCAAAACTGCGCATTTACGAAAACGCGAAAGTTTGCGTGGTTAATGCTGATGATGCCTTAACAATGCCGATTCGCGGTGCGGATGAACGCTGCGTCAGCTTTGGCGTCAACATGG  >  NC_000913/97607‑97792
                                                                                             |                                                                                            
CAGCGACCATTCTGAACGTGACTGAAGATCATATGGATCGCTATCCGTTTGGTTTACAACAGTATCGTGCAGCAAAACTGCGCATTTACGAAAG                                                                                              <  SRR4302537.415500/94‑1 (MQ=60)
 AGCGACCATTCTGAACGTGACTGAAGATCATATGGATCGCTATCCGTTTGGTTTACAACAGTATCGTGCAGCAAAACTGCGCATTTACGAAAGC                                                                                             >  SRR4302537.5397737/1‑94 (MQ=60)
 AGCGACCATTCTGAACGTGACTGAAGATCATATGGATCGCTATCCGTTTGGTTTACAACAGTATCGTGCAGCAAAACTGCGCATTTACGAAAGC                                                                                             <  SRR4302537.464643/94‑1 (MQ=60)
  GCGACCATTCTGAACGTGACTGAAGATCATATGGATCGCTATCCGTTTGGTTTACAACAGTATCGTGCAGCAAAACTGCGCATTTACGAAAGCG                                                                                            <  SRR4302537.2400912/94‑1 (MQ=60)
  GCGACCATTCTGAACGTGACTGAAGATCATATGGATCGCTATCCGTTTGGTTTACAACAGTATCGTGCAGCAAAACTGCGCATTTACGAAAGCG                                                                                            <  SRR4302537.454394/94‑1 (MQ=60)
   CGACCATTCTGAACGTGACTGAAGATCATATGGATCGCTATCCGTTTGGTTTACAACAGTATCGTGCAGCAAAACTGCGCATTTACGAAAGCGC                                                                                           >  SRR4302537.2172183/1‑94 (MQ=60)
    GACCATTCTGAACGTGACTGAAGATCATATGGATCGCTATCCGTTTGGTTTACAACAGTATCGTGCAGCAAAACTGCGCATTTACGAAAGCGCG                                                                                          <  SRR4302537.6123672/94‑1 (MQ=60)
    GACCATTCTGAACGTGACTGAAGATCATATGGATCGCTATCCGTTTGGTTTACAACAGTATCGTGCAGCAAAACTGCGCATTTACGAAAGCGCG                                                                                          >  SRR4302537.406254/1‑94 (MQ=60)
    GACCATTCTGAACGTGACTGAAGATCATATGGATCGCTATCCGTTTGGTTTACAACAGTATCGTGCAGCAAAACTGCGCATTTACGAAAGCGCG                                                                                          >  SRR4302537.2500930/1‑94 (MQ=60)
    GACCATTCTGAACGTGACTGAAGATCATATGGATCGCTATCCGTTTGGTTTACAACAGTATCGTGCAGCAAAACTGCGCATTTACGAAAGCGCG                                                                                          >  SRR4302537.278573/1‑94 (MQ=60)
      CCATTCTGAACGTGACTGAAGATCATATGGATCGCTATCCGTTTGGTTTACAACAGTATCGTGCAGCAAAACTGCGCATTTACGAAAGCGCGAA                                                                                        >  SRR4302537.3714622/1‑94 (MQ=60)
      CCATTCTGAACGTGACTGAAGATCATATGGATCGCTATCCGTTTGGTTTACAACAGTATCGTGCAGCAAAACTGCGCATTTACGAAAGCGCGAA                                                                                        <  SRR4302537.2004667/94‑1 (MQ=60)
       CATTCTGAACGTGACTGAAGATCATATGGATCGCTATCCGTTTGGTTTACAACAGTATCGTGCAGCAAAACTGCGCATTTACGAAAGCGCGAAA                                                                                       >  SRR4302537.3356940/1‑94 (MQ=60)
       CATTCTGAACGTGACTGAAGATCATATGGATCGCTATCCGTTTGGTTTACAACAGTATCGTGCAGCAAAACTGCGCATTTACGAAAGCGCGAAA                                                                                       <  SRR4302537.3633547/94‑1 (MQ=60)
       CATTCTGAACGTGACTGAAGATCATATGGATCGCTATCCGTTTGGTTTACAACAGTATCGTGCAGCAAAACTGCGCATTTACGAAAGCGCGAAA                                                                                       >  SRR4302537.1763593/1‑94 (MQ=60)
       CATTCTGAACGTGACTGAAGATCATATGGATCGCTATCCGTTTGGTTTACAACAGTATCGTGCAGCAAAACTGCGCATTTACGAAAGCGCGAAA                                                                                       >  SRR4302537.4822552/1‑94 (MQ=60)
        ATTCTGAACGTGACTGAAGATCATATGGATCGCTATCCGTTTGGTTTACAACAGTATCGTGCAGCAAAACTGCGCATTTACGAAAGCGCGAAAG                                                                                      <  SRR4302537.4668058/94‑1 (MQ=60)
        ATTCTGAACGTGACTGAAGATCATATGGATCGCTATCCGTTTGGTTTACAACAGTATCGTGCAGCAAAACTGCGCATTTACGAAAGCGCGAAAG                                                                                      <  SRR4302537.2269966/94‑1 (MQ=60)
        ATTCTGAACGTGACTGAAGATCATATGGATCGCTATCCGTTTGGTTTACAACAGTATCGTGCAGCAAAACTGCGCATTTACGAAAGCGCGAAAG                                                                                      >  SRR4302537.6155803/1‑94 (MQ=60)
           CTGAACGTGACTGAAGATCATATGGATCGCTATCCGTTTGGTTTACAACAGTATCGTGCAGCAAAACTGCGCATTTACGAAAGCGCGAAAGTTT                                                                                   >  SRR4302537.972876/1‑94 (MQ=60)
            TGAACGTGACTGAAGATCATATGGATCGCTATCCGTTTGGTTTACAACAGTATCGTGCAGCAAAACTGCGCATTTACGAAAGCGCGAAAGTTTG                                                                                  <  SRR4302537.1153621/94‑1 (MQ=60)
            TGAACGTGACTGAAGATCATATGGATCGCTATCCGTTTGGTTTACAACAGTATCGTGCAGCAAAACTGCGCATTTACGAAAGCGCGAAAGTTTG                                                                                  <  SRR4302537.4894586/94‑1 (MQ=60)
            TGAACGTGACTGAAGATCATATGGATCGCTATCCGTTTGGTTTACAACAGTATCGTGCAGCAAAACTGCGCATTTACGAAAGCGCGAAAGTTTG                                                                                  >  SRR4302537.329768/1‑94 (MQ=60)
             GAACGTGACTGAAGATCATATGGATCGCTATCCGTTTGGTTTACAACAGTATCGTGCAGCAAAACTGCGCATTTACGAAAGCGCGAAAGTTTGC                                                                                 <  SRR4302537.6133413/94‑1 (MQ=60)
             GAACGTGACTGAAGATCATATGGATCGCTATCCGTTTGGTTTACAACAGTATCGTGCAGCAAAACTGCGCATTTACGAAAGCGCGAAAGTTTGC                                                                                 >  SRR4302537.4938446/1‑94 (MQ=60)
             GAACGTGACTGAAGATCATATGGATCGCTATCCGTTTGGTTTACAACAGTATCGTGCAGCAAAACTGCGCATTTACGAAAGCGCGAAAGTTTGC                                                                                 <  SRR4302537.1333094/94‑1 (MQ=60)
               ACGTGACTGAAGATCATATGGATCGCTATCCGTTTGGTTTACAACAGTATCGTGCAGCAAAACTGCGCATTTACGAAAGCTCGAAAGTTTGCGT                                                                               >  SRR4302537.3715984/1‑94 (MQ=60)
               ACGTGACTGAAGATCATATGGATCGCTATCCGTTTGGTTTACAACAGTATCGTGCAGCAAAACTGCGCATTTACGAAAGCGCGAAAGTTTGCGT                                                                               >  SRR4302537.1195707/1‑94 (MQ=60)
               ACGTGACTGAAGATCATATGGATCGCTATCCGTTTGGTTTACAACAGTATCGTGCAGCAAAACTGCGCATTTACGAAAGCGCGAAAGTTTGCGT                                                                               <  SRR4302537.4161442/94‑1 (MQ=60)
                CGTGACTGAAGATCATATGGATCGCTATCCGTTTGGTTTACGACAGTATCGTGCAGCAAAGCTGCGCATTTACGAAAGCGCGAAAGTTTGCGTG                                                                              <  SRR4302537.820555/94‑1 (MQ=60)
                CGTGACTGAAGATCATATGGATCGCTATCCGTTTGGTTTACAACAGTATCGTGCAGCAAAACTGCGCATTTACGAAAGCGCGAAAGTTTGCGTG                                                                              <  SRR4302537.46708/94‑1 (MQ=60)
                CGTGACTGAAGATCATATGGATCGCTATCCGTTTGGTTTACAACAGTATCGTGCAGCAAAACTGCGCATTTACGAAAGCGCGAAAGTTTGCGTG                                                                              <  SRR4302537.3946385/94‑1 (MQ=60)
                CGTGACTGAAGATCATATGGATCGCTATCCGTTTGGTTTACAACAGTATCGTGCAGCAAAACTGCGCATTTACGAAAGCGCGAAAGTTTGCGTG                                                                              >  SRR4302537.2889069/1‑94 (MQ=60)
                 GTGACTGAAGATCATATGGATCGCTATCCGTTTGGTTTACAACAGTATCGTGCAGCAAAACTGCGCATTTACGAAAGCGCGAAAGTTTGCGTGG                                                                             <  SRR4302537.6149229/94‑1 (MQ=60)
                 GTGACTGAAGATCATATGGATCGCTATCCGTTTGGTTTACAACAGTATCGTGCAGCAAAACTGCGCATTTACGAAAGCGCGAAAGTTTGCGTGG                                                                             >  SRR4302537.222501/1‑94 (MQ=60)
                  TGACTGAAGATCATATGGATCGCTATCCGTTTGGTTTACAACAGTATCGTGCAGCAAAACTGCGCATTTACGAAAGCGCGAAAGTTTGCGTGGT                                                                            >  SRR4302537.4569421/1‑94 (MQ=60)
                  TGACTGAAGATCATATGGATCGCTATCCGTTTGGTTTACAACAGTATCGTGCAGCAAAACTGCGCATTTACGAAAGCGCGAAAGTTTGCGTGGT                                                                            >  SRR4302537.5672793/1‑94 (MQ=60)
                   GACTGAAGATCATATGGATCGCTATCCGTTTGGTTTACAACAGTATCGTGCAGCAAAACTGCGCATTTACGAAAGCGCGAAAGTTTGCGTGGTT                                                                           >  SRR4302537.5054244/1‑94 (MQ=60)
                   GACTGAAGATCATATGGATCGCTATCCGTTTGGTTTACAACAGTATCGTGCAGCAAAACTGCGCATTTACGAAAGCGCGAAAGTTTGCGTGGTT                                                                           >  SRR4302537.93806/1‑94 (MQ=60)
                      TGAAGATCATATGGATCGCTATCCGTTTGGTTTACAACAGTATCGTGCAGCAAAACTGCGCATTTACGAAAGCGCGAAAGTTTGCGTGGTTAAT                                                                        <  SRR4302537.2026995/94‑1 (MQ=60)
                       GAAGATCATATGGATCGCTATCCGTTTGGTTTACAACAGTATCGTGCAGCAAAACTGCGCATTTACGAAAGCGCGAAAGTTTGCGTGGTTAATG                                                                       <  SRR4302537.1743011/94‑1 (MQ=60)
                        AAGATCATATGGATCGCTATCCGTTTGGTTTACAACAGTATCGTGCAGCAAAACTGCGCATTTACGAAAGCGCGAAAGTTTGCGTGGTTAATGC                                                                      >  SRR4302537.4228562/1‑94 (MQ=60)
                        AAGATCATATGGATCGCTATCCGTTTGGTTTACAACAGTATCGTGCAGCAAAACTGCGCATTTACGAAAGCGCGAAAGTTTGCGTGGTTAATGC                                                                      >  SRR4302537.3841993/1‑94 (MQ=60)
                         AGATCATATGGATCGCTATCCGTTTGGTTTACAACAGTATCGTGCAGCAAAACTGCGCATTTACGAAAGCGCGAAAGTTTGCGTGGTTAATGCT                                                                     >  SRR4302537.1931542/1‑94 (MQ=60)
                          GATCATATGGATCGCTATCCGTTTGGTTTACAACAGTATCGTGCAGCAAAACTGCGCATTTACGAAAGCGCGAAAGTTTGCGTGGTTAATGCTG                                                                    >  SRR4302537.3255532/1‑94 (MQ=60)
                           ATCATATGGATCGCTATCCGTTTGGTTTACAACAGTATCGTGCAGCAAAACTGCGCATTTACGAAAGCGCGAAAGTTTGCGTGGTTAATGCTGA                                                                   <  SRR4302537.4516060/94‑1 (MQ=60)
                            TCATATGGATCGCTATCCGTTTGGTTTACAACAGTATCGTGCAGCAAAACTGCGCATTTACGAAAGCGCGAAAGTTTGCGTGGTTAATGCTGAT                                                                  <  SRR4302537.5520964/94‑1 (MQ=60)
                            TCATATGGATCGCTATCCGTTTGGTTTACAACAGTATCGTGCAGCAAAACTGCGCATTTACGAAAGCGCGAAAGTTTGCGTGGTTAATGCTGAT                                                                  <  SRR4302537.3270252/94‑1 (MQ=60)
                            TCATATGGATCGCTATCCGTTTGGTTTACAACAGTATCGTGCAGCAAAACTGCGCATTTACGAAAGCGCGAAAGTTTGCGTGGTTAATGCTGAT                                                                  <  SRR4302537.735563/94‑1 (MQ=60)
                            TCATATGGATCGCTATCCGTTTGGTTTACAACAGTATCGTGCAGCAAAACTGCGCATTTACGAAAGCGCGAAAGTTTGCGTGGTTAATGCTGAT                                                                  >  SRR4302537.5303100/1‑94 (MQ=60)
                             CATATGGATCGCTATCCGTTTGGTTTACAACAGTATCGTGCAGCAAAACTGCGCATTTACGAAAGCGCGAAAGTTTGCGTGGTTAATGCTGATG                                                                 >  SRR4302537.3158748/1‑94 (MQ=60)
                             CATATGGATCGCTATCCGTTTGGTTTACAACAGTATCGTGCAGCAAAACTGCGCATTTACGAAAGCGCGAAAGTTTGCGTGGTTAATGCTGATG                                                                 <  SRR4302537.1939628/94‑1 (MQ=60)
                              ATATGGATCGCTATCCGTTTGGTTTACAACAGTATCGTGCAGCAAAACTGCGCATTTACGAAAGCGCGAAAGTTTGCGTGGTTAATGCTGATGA                                                                >  SRR4302537.5290018/1‑94 (MQ=60)
                               TATGGATCGCTATCCGTTTGGTTTACAACAGTATCGTGCAGCAAAACTGCGCATTTACGAAAGCGCGAAAGTTTGCGTGGTTAATGCTGATGAT                                                               >  SRR4302537.1244655/1‑94 (MQ=60)
                               TATGGATCGCTATCCGTTTGGTTTACAACAGTATCGTGCAGCAAAACTGCGCATTTACGAAAGCGCGAAAGTTTGCGTGGTTAATGCTGATGAT                                                               <  SRR4302537.1672173/94‑1 (MQ=60)
                               TATGGATCGCTATCCGTTTGGTTTACAACAGTATCGTGCAGCAAAACTGCGCATTTACGAAAGCGCGAAAGTTTGCGTGGTTAATGCTGATGAT                                                               <  SRR4302537.3297246/94‑1 (MQ=60)
                                ATGGATCGCTATCCGTTTGGTTTACAACAGTATCGTGCAGCAAAACTGCGCATTTACGAAAGCGCGAAAGTTTGCGTGGTTAATGCTGATGATG                                                              <  SRR4302537.83162/94‑1 (MQ=60)
                                ATGGATCGCTATCCGTTTGGTTTACAACAGTATCGTGCAGCAAAACTGCGCATTTACGAAAGCGCGAAAGTTTGCGTGGTTAATGCTGATGATG                                                              <  SRR4302537.2449152/94‑1 (MQ=60)
                                 TGGATCGCTATCCGTTTGGTTTACAACAGTATCGTGCAGCAAAACTGCGCATTTACGAAAGCGCGAAAGTTTGCGTGGTTAATGCTGATGATGC                                                             <  SRR4302537.5268995/94‑1 (MQ=60)
                                  GGATCGCTATCCGTTTGGTTTACAACAGTATCGTGCAGCAAAACTGCGCATTTACGAAAGCGCGAAAGTTTGCGTGGTTAATGCTGATGATGCC                                                            >  SRR4302537.1547590/1‑94 (MQ=60)
                                    ATCGCTATCCGTTTGGTTTACAACAGTATCGTGCAGCAAAACTGCGCATTTACGAAAGCGCGAAAGTTTGCGTGGTTAATGCTGATGATGCCTT                                                          <  SRR4302537.1226170/94‑1 (MQ=60)
                                      CGCTATCCGTTTGGTTTACAACAGTATCGTGCAGCAAAACTGCGCATTTACGAAAGCGCGAAAGTTTGCGTGGTTAATGCTGATGCTGCCTTAA                                                        >  SRR4302537.4339274/1‑94 (MQ=60)
                                      CGCTATCCGTTTGGTTTACAACAGTATCGTGCAGCAAAACTGCGCATTTACGAAAGCGCGAAAGTTTGCGTGGTTAATGCTGATGATGCCTTAA                                                        >  SRR4302537.3717676/1‑94 (MQ=60)
                                      CGCTATCCGTTTGGTTTACAACAGTATCGTGCAGCAAAACTGCGCATTTACGAAAGAGCGAAAGTTTGCGTGGTTAATGCTGATGATGCCTTAA                                                        >  SRR4302537.2124007/1‑94 (MQ=60)
                                       GCGATCCGTTTGGTTTACAACAGTATCGTGCAGCAAAACTGCGCATTTACGAAAGCGCGAAAGTTTGCGTGGTTAATGCTGATGATGCCTTAAC                                                       >  SRR4302537.6292534/1‑94 (MQ=60)
                                         TATCCGTTTGGTTTACAACAGTATCGTGCAGCAAAACTGCGCATTTACGAAAGCGCGAAAGTTTGCGTGGTTAATGCTGATGATGCCTTAACAA                                                     >  SRR4302537.1857984/1‑94 (MQ=60)
                                          ATCCGTTTGGTTTACAACAGTATCGTGCAGCAAAACTGCGCATTTACGAAAGCGCGAAAGTTTGCGTGGTTAATGCTGATGATGCCTTAACAAT                                                    >  SRR4302537.5728731/1‑94 (MQ=60)
                                           TCCGTTTGGTTTACAACAGTATCGTGCAGCAAAACTGCGCATTTACGAAAGCGCGAAAGTTTGCGTGGTTAATGCTGATGATGCCTTAACAATG                                                   <  SRR4302537.6421633/94‑1 (MQ=60)
                                            CCGTTTGGTTTACAACAGTATCGTGCAGCAAAACTGCGCATTTACGAAAGCGCGAAAGTTTGCGTGGTTAATGCTGATGATGCCTTAACAATGC                                                  >  SRR4302537.27236/1‑94 (MQ=60)
                                            CCGTTTGGTTTACAACAGTATCGTGCAGCAAAACTGCGCATTTACGAAAGCGCGAAAGTTTGCGTGGTTAATGCTGATGATGCCTTAACAATGC                                                  >  SRR4302537.5400773/1‑94 (MQ=60)
                                            CCGTTTGGTTTACAACAGTATCGTGCAGCAAAACTGCGCATTTACGAAAGCGCGAAAGTTTGCGTGGTTAATGCTGATGATGCCTTAACAATGC                                                  <  SRR4302537.5016893/94‑1 (MQ=60)
                                            CCGTTTGGTTTACAACAGTATCGTGCAGCAAAACTGCGCATTTACGAAAGCGCGAAAGTTTGCGTGGTTAATGCTGATGATGCCTTAACAATGC                                                  >  SRR4302537.3191140/1‑94 (MQ=60)
                                              GTTTGGTTTACAACAGTATCGTGCAGCAAAACTGCGCATTTACGAAAGCGCGAAAGTTTGCGTGGTTAATGCTGATGATGCCTTAACAATGCCG                                                >  SRR4302537.6101136/1‑94 (MQ=60)
                                              GTTTGGTTTACAACAGTATCGTGCAGCAAAACTGCGCATTTACGAAAGCGCGAAAGTTTGCGTGGTTAATGCTGATGATGCCTTAACAATGCCG                                                >  SRR4302537.4641623/1‑94 (MQ=60)
                                                TTGGTTTACAACAGTATCGTGCAGCAAAACTGCGCATTTACGAAAGCGCGAAAGTTTGCGTGGTTAATGCTGATGATGCCTTAACAATGCCGAT                                              <  SRR4302537.3383820/94‑1 (MQ=60)
                                                   GTTTACAACAGTATCGTGCAGCAAAACTGCGCATTTACGAAAGCGCGAAAGTTTGCGTGGTTAATGCTGATGATGCCTTAACAATGCCGATTCG                                           >  SRR4302537.4654652/1‑94 (MQ=60)
                                                    TTTACAACAGTATCGTGCAGCAAAACTGCGCATTTACGAAAGCGCGAAAGTTTGCGTGGTTAATGCTGATGATGCCTTAACAATGCCGATTCGC                                          >  SRR4302537.4007959/1‑94 (MQ=60)
                                                    TTTACAACAGTATCGTGCAGCAAAACTGCGCATTTACGAAAGCGCGAAAGTTTGCGTGGTTAATGCTGATGATGCCTTAACAATGCCGATTCGC                                          >  SRR4302537.4368918/1‑94 (MQ=60)
                                                     TTACAACAGTATCGTGCAGCAAAACTGCGCATTTACGAAAGCGCGAAAGTTTGCGTGGTTAATGCTGATGATGCCTTAACAATGCCGATTCGCG                                         >  SRR4302537.730296/1‑94 (MQ=60)
                                                     TTACAACAGTATCGTGCAGCAAAACTGCGCATTTACGAAAGCGCGAAAGTTTGCGTGGTTAATGCTGATGATGCCTTAACAATGCCGATTCGCG                                         >  SRR4302537.5599370/1‑94 (MQ=60)
                                                      TACAACAGTATCGTGCAGCAAAACTGCGCATTTACGAAAGCGCGAAAGTTTGCGTGGTTAATGCTGATGATGCCTTAACAATGCCGATTCGCGG                                        >  SRR4302537.2166704/1‑94 (MQ=60)
                                                      TACAACAGTATCGTGCAGCAAAACTGCGCATTTACGAAAGCGCGAAAGTTTGCGTGGTTAATGCTGATGATGCCTTAACAATGCCGATTCGCGG                                        <  SRR4302537.5797931/94‑1 (MQ=60)
                                                      TACAACAGTATCGTGCAGCAAAACTGCGCATTTACGAAAGCGCGAAAGTTTGCGTGGTTAATGCTGATGATGCCTTAACAATGCCGATTCGCGG                                        >  SRR4302537.1871535/1‑94 (MQ=60)
                                                      TACAACAGTATCGTGCAGCAAAACTGCGCATTTACGAAAGCGCGAAAGTTTGCGTGGTTAATGCTGATGATGCCTTAACAATGCCGATTCGCGG                                        <  SRR4302537.1696775/94‑1 (MQ=60)
                                                      TACAACAGTATCGTGCAGCAAAACTGCGCATTTACGAAAGCGCGAAAGTTTGCGTGGTTAATGCTGATGATGCCTTAACAATGCCGATTCGCGG                                        >  SRR4302537.869596/1‑94 (MQ=60)
                                                       ACAACAGTATCGTGCAGCAAAACTGCGCATTTACGCAAGCGCGAAAGTTTGCGTGGTTAATGCTGATGATGCCTTAACAATGCCGATTCGCGGT                                       <  SRR4302537.4257772/94‑1 (MQ=60)
                                                       ACAACAGTATCGTGCAGCAAAACTGCGCATTTACGAAAGCGCGAAAGTTTGCGTGGTTAATGCTGATGATGCCTTAACAATGCCGATTCGCGGT                                       >  SRR4302537.2826006/1‑94 (MQ=60)
                                                        CAACAGTATCGTGCAGCAAAACTGCGCATTTACGAAAGCGCGAAAGTTTGCGTGGTTAATGCTGATGATGCCTTAACAATGCCGATTCGCGGTG                                      >  SRR4302537.3991266/1‑94 (MQ=60)
                                                         AACAGTATCGTGCAGCAAAACTGCGCATTTACGAAAGCGCGAAAGTTTGCGTGGGTAATGCTGATGATGCCTTAACAATGCCGATTCGCGGTGC                                     >  SRR4302537.292272/1‑94 (MQ=60)
                                                          ACAGTATCGTGCAGCAAAACTGCGCATTTACGAAAGCGCGAAAGTTTGCGTGGTTAATGCTGATGATGCCTTAACAATGCCGATTCGCGGTGCG                                    >  SRR4302537.3542855/1‑94 (MQ=60)
                                                           CAGTATCGTGCAGCAAAACTGCGCATTTACGAAAGCGCGAAAGTTTGCGTGGTTAATGCTGATGATGCCTTAACAATGCCGATTCGCGGTGCGG                                   <  SRR4302537.4400899/94‑1 (MQ=60)
                                                            AGTATCGTGCAGCAAAACTGCGCATTTACGAAAGCGCGAAAGTTTGCGTGGTTAATGCTGATGATGCCTTAACAATGCCGATTCGCGGTGCGGA                                  >  SRR4302537.2289043/1‑94 (MQ=60)
                                                            AGTATCGTGCAGCAAAACTGCGCATTTACGAAAGCGCGAAAGTTTGCGTGGTTAATGCTGATGATGCCTTAACAATGCCGATTCGCGGTGCGGA                                  >  SRR4302537.6027128/1‑94 (MQ=60)
                                                            AGTATCGTGCAGCAAAACTGCGCATTTACGAAAGCGCGAAAGTTTGCGTGGTTAATGCTGATGATGCCTTAACAATGCCGATTCGCGGTGCGGA                                  >  SRR4302537.3809719/1‑94 (MQ=60)
                                                             GTATCGTGCAGCAAAACTGCGCATTTACGAAAGCGCGAAAGTTTGCGTGGTTAATGCTGATGATGCCTTAACAATGCCGATTCGCGGTGCGGAT                                 <  SRR4302537.1150694/94‑1 (MQ=60)
                                                              TATCGTGCAGCAAAACTGCGCATTTACGAAAGCGCGAAAGTTTGCGTGGTTAATGCTGATGATGCCTTAACAATGCCGATTCGCGGTGCGGATG                                >  SRR4302537.5224039/1‑94 (MQ=60)
                                                              TATCGTGCAGCAAAACTGCGCATTTACGAAAGCGCGAAAGTTTGCGTGGTTAATGCTGATGATGCCTTAACAATGCCGATTCGCGGTGCGGATG                                <  SRR4302537.2403635/94‑1 (MQ=60)
                                                               ATCGTGCAGCAAAACTGCGCATTTACGAAAGCGCGAAAGTTTGCGTGGTTAATGCTGATGATGCCTTAACAATGCCGATTCGCGGTGCGGATGA                               >  SRR4302537.137329/1‑94 (MQ=60)
                                                                TCGTGCAGCAAAACTGCGCATTTACGAAAGCGCGAAAGTTTGCGTGGTTAATGCTGATGATGCCTTAACAATGCCGATTCGCGGTGCGGATGAA                              <  SRR4302537.4096342/94‑1 (MQ=60)
                                                                 CGTGCAGCAAAACTGCGCATTTACGAAAGCGCGAAAGTTTGCGTGGTTAATGCTGATGATGCCTTAACAATGCCGATTCGCGGTGCGGATGAAC                             >  SRR4302537.5771195/1‑94 (MQ=60)
                                                                  GTGCAGCAAAACTGCGCATTTACGAAAGCGCGAAAGTTTGCGTGGTTAATGCTGATGATGCCTTAACAATGCCGATTCGCGGTGCGGATGAACG                            <  SRR4302537.3972772/94‑1 (MQ=60)
                                                                   TGCAGCAAAACTGCGCATTTACGAAAGCGCGAAAGTTTGCGTGGTTAATGCTGATGATGCCTTAACAATGCCGATTCGCGGTGCGGATGAACGC                           >  SRR4302537.4280208/1‑94 (MQ=60)
                                                                     CAGCAAAACTGCGCATTTACGAAAGCGCGAAAGTTTGCGTGGTTAATGCTGATGATGCCTTAACAATGCCGATTCGCGGTGCGGATGAACGCTG                         >  SRR4302537.6193552/1‑94 (MQ=60)
                                                                      AGCAAAACTGCGCATTTACGAAAGCGCGAAAGTTTGCGTGGTTAATGCTGATGATGCCTTAACAATGCCGATTCGCGGTGCGGATGAACGCTGC                        >  SRR4302537.452017/1‑94 (MQ=60)
                                                                      AGCAAAACTGCGCATTTACGAAAGCGCGAAAGTTTGCGTGGTTAATGCTGATGATGCCTTAACAATGCCGATTCGCGGTGCGGATGAACGCTGC                        <  SRR4302537.2792057/94‑1 (MQ=60)
                                                                      AGCAAAACTGCGCATTTACGAAAGCGCGAAAGTTTGCGTGGTTAATGCTGATGATGCCTTAACAATGCCGATTCGCGGTGCGGATGAACGCTGC                        >  SRR4302537.5478240/1‑94 (MQ=60)
                                                                      AGCAAAACTGCGCATTTACGAAAGCGCGAAAGTTTGCGTGGTTAATGCTGATGATGCCTTAACAATGCCGATTCGCGGTGCGGATGAACGCTGC                        >  SRR4302537.4553460/1‑94 (MQ=60)
                                                                       GCAAAACTGCGCATTTACGAAAGCGCGAAAGTTTGCGTGGTTAATGCTGATGATGCCTTAACAATGCCGATTCGCGGTGCGGATGAACGCTGCG                       <  SRR4302537.1603410/94‑1 (MQ=60)
                                                                        CAAAACTGCGCATTTACGAAAGCGCGAAAGTTTGCGTGGTTAATGCTGATGATGCCTTAACAATGCCGATTCGCGGTGCGGATGAACGCTGCGT                      >  SRR4302537.4345560/1‑94 (MQ=60)
                                                                        CAAAACTGCGCATTTACGAAAGCGCGAAAGTTTGCGTGGTTAATGCTGATGATGCCTTAACAATGCCGATTCGCGGTGCGGATGAACGCTGCGT                      >  SRR4302537.4189107/1‑94 (MQ=60)
                                                                        CAAAACTGCGCATTTACGAAAGCGCGAAAGTTTGCGTGGTTAATGCTGATGATGCCTTAACAATGCCGATTCGCGGTGCGGATGAACGCTGCGT                      >  SRR4302537.3831223/1‑94 (MQ=60)
                                                                         AAAACTGCGCATTTACGAAAGCGCGAAAGTTTGCGTGGTTAATGCTGATGATGCCTTAACAATGCCGATTCGCGGTGCGGATGAACGCTGCGTC                     <  SRR4302537.6058440/94‑1 (MQ=60)
                                                                            ACTGCGCATTTACGAAAGCGCGAAAGTTTGCGTGGTTAATGCTGATGATGCCTTAACAATGCCGATTCGCGGTGCGGATGAACGCTGCGTCAGC                  <  SRR4302537.5521409/94‑1 (MQ=60)
                                                                             CTGCGCATTTACGAAAGCGCGAAAGTTTGCGTGGTTAATGCTGATGATGCCTTAACAATGCCGATTCGCGGTGCGGATGAACGCTGCGTCAGCT                 >  SRR4302537.4889105/1‑94 (MQ=60)
                                                                             CTGCGCATTTACGAAAGCGCGAAAGTTTGCGTGGTTAATGCTGATGATGCCTTAACAATGCCGATTCGCGGTGCGGATGAACGCTGCGTCAGCT                 >  SRR4302537.3522013/1‑94 (MQ=60)
                                                                              TGCGCATTTACGAAAGCGCGAAAGTTTGCGTGGTTAATGCTGATGATGCCTTAACAATGCCGATTCGCGGTGCGGATGAACGCTGCGTCAGCTT                >  SRR4302537.111055/1‑94 (MQ=60)
                                                                              TGCGCATTTACGAAAGCGCGAAAGTTTGCGTGGTTAATGCTGATGATGCCTTAACAATGCCGATTCGCGGTGCGGATGAACGCTGCGTCAGCTT                <  SRR4302537.1186591/94‑1 (MQ=60)
                                                                              TGCGCATTTACGAAAGCGCGAAAGTTTGCGTGGTTAATGCTGATGATGCCTTAACAATGCCGATTCGCGGTGCGGATGAACGCTGCGTCAGCTT                >  SRR4302537.1575107/1‑94 (MQ=60)
                                                                                  CATTTACGAAAGCGCGAAAGTTTGCGTGGTTAATGCTGATGATGCCTTAACAATGCCGATTCGCGGTGCGGATGAACGCTGCGTCAGCTTTGGC            <  SRR4302537.1981585/94‑1 (MQ=60)
                                                                                   ATTTACGAAAGCGCGAAAGTTTGCGTGGTTAATGCTGATGATGCCTTAACAATGCCGATTCGCGGTGCGGATGAACGCTGCGTCAGCTTTGGCG           >  SRR4302537.3814852/1‑94 (MQ=60)
                                                                                   ATTTACGAAAGCGCGAAAGTTTGCGTGGTTAATGCTGATGATGCCTTAACAATGCCGATTCGCGGTGCGGATGAACGCTGCGTCAGCTTTGGCG           <  SRR4302537.2708529/94‑1 (MQ=60)
                                                                                    TTTACGAAAGCGCGAAAGTTTGCGTGGTTAATGCTGATGATGCCTTAACAATGCCGATTCGCGGTGCGGATGAACGCTGCGTCAGCTTTGGCGT          >  SRR4302537.2000050/1‑94 (MQ=60)
                                                                                     TTACGAAAGCGCGAAAGTTTGCGTGGTTAATGCTGATGATGCCTTAACAATGCCGATTCGCGGTGCGGATGAACGCTGCGTCAGCTTTGGCGTC         <  SRR4302537.4359085/94‑1 (MQ=60)
                                                                                      TACGAAAGCGCGAAAGTTTGCGTGGTTAATGCTGATGATGCCTTAACAATGCCGATTCGCGGTGCGGATGAACGCTGCGTCAGCTTTGGCGTCA        >  SRR4302537.4299622/1‑94 (MQ=60)
                                                                                       ACGAAAGCGCGAAAGTTTGCGTGGTTAATGCTGATGATGCCTTAACAATGCCGATTCGCGGTGCGGATGAACGCTGCGTCAGCTTTGGCGTCAA       >  SRR4302537.2391344/1‑94 (MQ=60)
                                                                                        CGAAAGCGCGAAAGTTTGCGTGGTTAATGCTGATGATGCCTTAACAATGCCGATTCGCGGTGCGGATGAACGCTGCGTCAGCTTTGGCGTCAAC      <  SRR4302537.782174/94‑1 (MQ=60)
                                                                                         GAAAGCGCGAAAGTTTGCGTGGTTAATGCTGATGATGCCTTAACAATGCCGATTCGCGGTGCGGATGAACGCTGCGTCAGCTTTGGCGTCAACA     >  SRR4302537.3445649/1‑94 (MQ=60)
                                                                                         GAAAGCGCGAAAGTTTGCGTGGTTAATGCTGATGATGCCTTAACAATGCCGATTCGCGGTGCGGATGAACGCTGCGTCAGCTTTGGCGTCAACA     >  SRR4302537.942205/1‑94 (MQ=60)
                                                                                          AAAGCGCGAAAGTTTGCGTGGTTAATGCTGATGATGCCTTAACAATGCCGATTCGCGGTGCGGATGAACGCTGCGTCAGCTTTGGCGTCAACAT    <  SRR4302537.765215/94‑1 (MQ=60)
                                                                                            AGCGCGAAAGTTTGCGTGGTTAATGCTGATGATGCCTTAACAATGCCGATTCGCGGTGCGGATGAACGCTGCGTCAGCTTTGGCGTCAACATGG  >  SRR4302537.6070624/1‑94 (MQ=60)
                                                                                             |                                                                                            
CAGCGACCATTCTGAACGTGACTGAAGATCATATGGATCGCTATCCGTTTGGTTTACAACAGTATCGTGCAGCAAAACTGCGCATTTACGAAAACGCGAAAGTTTGCGTGGTTAATGCTGATGATGCCTTAACAATGCCGATTCGCGGTGCGGATGAACGCTGCGTCAGCTTTGGCGTCAACATGG  >  NC_000913/97607‑97792

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑