Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I2 R1 882 254.4 12957588 98.0% 12698436 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 375,783 T→C T382T (ACT→ACC)  mhpT → putative transport protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913375,7830TC96.5% 547.8 / 7.0 169T382T (ACT→ACC) mhpTputative transport protein
Reads supporting (aligned to +/- strand):  ref base T (2/4);  new base C (77/86);  total (79/90)
Fisher's exact test for biased strand distribution p-value = 6.86e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.84e-01

Only 100 of 174 total aligned reads displayed.

CGCAACAGGTGTGGGAACAGCCGTGGCGGTAGGGCGTCTGGGGGCTATGAGCGGTCCGTTACTGGCCGGGAAAATGCTGGCATTAGGCACTGGCACGGTCGGCGTAATGGCCGCTTCTGCACCGGGTATTCTTGTTGCTGGGTTGGCGGTGTTTATTTTGATGAGCCGGAGATCACGAATACA  >  NC_000913/375693‑375875
                                                                                          |                                                                                            
cgcAACAGGTGTGGGAACAGCCGTGGCGGTAGGGCGTCTGGGGGCTATGAGCGGTCCGTTACTGGCCGGGAAAATGCTGGCATTACGCACCgg                                                                                            <  2:5232809/93‑1 (MQ=255)
  cAACAGGTGTGGGAACAGCCGTGGCGGTAGGTCGTCTGGGGGCTATGAGCGGTCCGTTACTGGCCGGGAAAATTCTGGCATTAGGCACCGGCa                                                                                          <  2:2578931/93‑1 (MQ=255)
  cAACAGGTGTGGGAACAGCCGTGGCGGTAGGGCGTCTGGGGGCTATGAGCGGTCCGTTACTGGCCGGGAAAATGCTGGCATTAGGCACCGGCa                                                                                          <  2:1628106/93‑1 (MQ=255)
    aCAGGTGTGGGAACAGCCGTGGCGGTAGGGCGTCTGGGGGCTATGAGCGGTCCGTTACTGGCCGGGAAAATGCTGGCATTAGGCACCGGCACg                                                                                        >  2:6460303/1‑93 (MQ=255)
     cAGGTGTGGGAACAGCCGTGGCGGTAGGGCGTCTGGGGGCTATGAGCGGTCCGTTACTGGCCGGGAAAATGCTGGCATTAGGCACCGGCACgg                                                                                       <  1:2241046/93‑1 (MQ=255)
     cAGGTGTGGGAACAGCCGTGGCGGTAGGGCGTCTGGGGGCTATGAGCGGTCCGTTACTGGCCGGGAAAATGCTGGCATTAGGCACCGGCACgg                                                                                       <  2:5687013/93‑1 (MQ=255)
      aGGTGTGGGAACAGCCGTGGCGGTAGGGCGTCTGGGGGCTATGAGCGGTCCGTTACTGGCCGGGAAAATGCTGGCATTAGGCACCGGCACGGt                                                                                      <  1:5833353/93‑1 (MQ=255)
      aGGTGTGGGAACAGCCGTGGCGGTAGGGCGTCTGGGGGCTATGAGCGGTCCGTTACTGGCCGGGAAAATGCTGGCATTAGGCACCGGCACGGt                                                                                      <  2:4942680/93‑1 (MQ=255)
       ggTGTGGGAACAGCCGTGGCGGTAGGGCGTCTGGGGGCTATGAGCGGTCCGTTACTGGCCGGGAAAATGCTGGCATTAGGCACCGGCACGGTc                                                                                     >  2:2771841/1‑93 (MQ=255)
       ggTGTGGGAACAGCCGTGGCGGTAGGGCGTCTGGGGGCTATGAGCGGTCCGTTACTGGCCGGGAAAATGCTGGCATTAGGCACCGGCACGGTc                                                                                     >  2:3697563/1‑93 (MQ=255)
       ggTGTGGGAACAGCCGTGGCGGTAGGGCGTCTGGGGGCTATGAGCGGTCCGTTACTGGCCGGGAAAATGCTGGCATTAGGCACCGGCACGGTc                                                                                     >  2:4164378/1‑93 (MQ=255)
       ggTGTGGGAACAGCCGTGGCGGTAGGGCGTCTGGGGGCTATGAGCGGTCCGTTACTGGCCGGGAAAATGCTGGCATTAGGCACCGGCACGGTc                                                                                     >  1:3129194/1‑93 (MQ=255)
         tgtgGGAACAGCCGTGGCGGTAGGGCGTCTGGGGGCTATGAGCGGTCCGTTACTGGCCGGGAAAATGCTGGCATTAGGCACCGGCACGGTCgg                                                                                   >  2:1025805/1‑93 (MQ=255)
            gggAACAGCCGTGGCGGTAGGGCGTCTGGGGGCTATGAGCGGTCCGTTACTGGCCGGGAAAATGCTGGCATTAGGCACCGGCACGGTCGGCGt                                                                                >  1:1437924/1‑93 (MQ=255)
              gAACAGCCGTGGCGGTAGGGCGTCTGGGGGCTATGAGCGGTCCGTTACTGGCCGGGAAAATGCTGGCATTAGGCACCGGCACGGTCGGCGTaa                                                                              <  2:2375626/93‑1 (MQ=255)
                aCAGCCGTGGCGGTAGGGCGTCTGGGGGCTATGAGCGGTCCGTTACTGGCCGGGAAAATGCTGGCATTAGGCACCGGCACGGTCGGCGTAATg                                                                            <  2:4768831/93‑1 (MQ=255)
                aCAGCCGTGGCGGGAGGGCGTCTGGGGGCTATGAGCGGTCCGTTACTGGCCGGGAAAATGCTGGCATTAGGCACCGGCACGGTCGGCGTAATg                                                                            >  2:4145811/1‑93 (MQ=255)
                  aGCCGTGGCGGTAGGGCGTCTGGGGGCTATGAGCGGTCCGTTACTGGCCGGGAAAATGCTGGCATTAGGCACCGGCACGGTCGGCGTAATGGc                                                                          >  2:5736722/1‑93 (MQ=255)
                   gCCGTGGCGGTAGGGCGTCTGGGGGCTATGAGCGGTCCGTTACTGGCCGGGAAAATGCTGGCATTAGGCACCGGCACGGTCGGCGTAATGGcc                                                                         >  2:5627817/1‑93 (MQ=255)
                     cGTGGCGGTAGGGCGTCTGGGGGCTATGAGCGGTCCGTTACTGGCCGGGAAAATGCTGGCATTAGGCACCGGCACGGTCGGCGTAATGGCCGc                                                                       <  1:5331095/93‑1 (MQ=255)
                     cGTGGCGGTAGGGCGTCTGGGGGCTATGAGCGGTCCGTTACTGGCCGGGAAAATGCTGGCATTAGGCACCGGCACGGTCGGCGTAATGGCCGc                                                                       <  1:1003464/93‑1 (MQ=255)
                      gTGGCGGTAGGGCGTCTGGGGGCTATGAGCGGTCCGTTACTGGCCGGGAAAATGCTGGCATTAGGCACCGGCACGGTCGGCGTAATGGCCGCt                                                                      <  2:67796/93‑1 (MQ=255)
                       tGGCGGTAGGGCGTCTGGGGGCTATGAGCGGTCCGTTACTGGCCGGGAAAATGCTGGCATTAGGCACCGGCACGGTCGGCGTAATGGCCGCtt                                                                     >  2:1624036/1‑93 (MQ=255)
                       tGGCGGTAGGGCGTCTGGGGGCTATGAGCGGTCCGTTACTGGCCGGGAAAATGCTGGCATTAGGCACCGGCACGGTCGGCGTAATGGCCGCtt                                                                     >  2:2816495/1‑93 (MQ=255)
                        ggCGGTAGGGCGTCTGGGGGCTATGAGCGGTCCGTTACTGGCCGGGAAAATGCTGGCATTAGGCACCGGCACGGTCGGCGTAATGGCCGCTTc                                                                    <  2:4297624/93‑1 (MQ=255)
                            gTAGGGCGTCTGGGGGCTATGAGCGGTCCGTTACTGGCCGGGAAAATGCTGGCATTAGGCACCGGCACGGTCGGCGTAATGGCCGCTTCTGCa                                                                >  2:2663546/1‑93 (MQ=255)
                             tatggCGTCTGGGGGCTATGAGCGGTCCGTTACTGGCCGGGAAAATGCTGGCATTAGGCACCGGCACGGTCGGCGTAATGGCCGCTTCTGCAc                                                               <  2:5901831/90‑1 (MQ=255)
                                ggCGTCTGGGGGCTATGAGCGGTCCGTTACTGGCCGGGAAAATGCTGGCATTAGGCACCGGCACGGTCGGCGTAATGGCCGCTTCTGCACCgg                                                            >  1:1970974/1‑93 (MQ=255)
                                 gCGTCTGGGGGCTATGAGCGGTCCGTTACTGGCCGGGAAAATGCTGGCATTAGGCACCGGCACGGTCGGCGTAATGGCCGCTTCTGCACCggg                                                           <  2:1433371/93‑1 (MQ=255)
                                   gTCTGGGGGCTATGAGCGGTCCGTTACTGGCCGTGAAAATGCTGGCATTAGGCACCGGCACGGTCGGCGTAATGGCCGCTTCTGCACCGGGTa                                                         <  1:4640557/93‑1 (MQ=255)
                                    tCTGGGGGCTATGAGCGGTCCGTTACTGGCCGGGAAAATGCTGGCATTAGGCACCGGCACGGTCGGCGTAATGGCCGCTTCTGCACCGGGTAt                                                        <  2:792868/93‑1 (MQ=255)
                                    tCTGGGGGCTATGAGCGGTCCGTTACTGGCCGGGAAAATGCTGGCATTAGGCACCGGCACGGTCGGCGTAATGGCCGCTTCTGCACCGGGTAt                                                        >  2:5233708/1‑93 (MQ=255)
                                    tCTGGGGGCTATGAGCGGTCCGTTACTGGCCGGGAAAATGCTGGCATTAGGCACCGGCACGGTCGGCGTAATGGCCGCTTCTGCACCGGGTAt                                                        >  2:2207790/1‑93 (MQ=255)
                                      tGGGGGCTATGAGCGGTCCGTTACTGGCCGGGAAAATGCTGGCATTAGGCACCGGCACGGTCGGCGTAATGGCCGCTTCTGCACCGGGTGTTc                                                      >  2:1785875/1‑93 (MQ=255)
                                       gggggCTATGAGCGGTCCGTTACTGGCCGGGAAAATGCTGGCATTAGGCACCGGCACGGTCGGCGTAATGGCCGCTTCTGCACCGGGTATTCt                                                     >  2:2940262/1‑93 (MQ=255)
                                        ggggCTATGAGCGGTCCGTTACTGGCCGGGAAAATGCTGGCATTAGGCACCGGCACGGTCGGCGTAATGGCCGCTTCTGCACCGGGTATTCtt                                                    <  2:4012696/93‑1 (MQ=255)
                                          ggCTATGAGCGGTCCGTTACTGGCCGGGAAAATGCTGGCATTAGGCACCGGCACGGTCGGCGTAATGGCCGCTTCTGCACCGGGTATTCttgt                                                  >  2:765077/1‑93 (MQ=255)
                                          ggCTATGAGCGGTCCGTTACTGGCCGGGAAAATGCTGGCATTAGGCACCGGCACGGTCGGCGTAATGGCCGCTTCTGCACCGGGTATTCttgt                                                  <  1:2905379/93‑1 (MQ=255)
                                           gCTATGAGCGGTCCGTTACTGGCCGGGAAAATGCTGGCATTAGGCACCGGCACGGTCGGCGTAATGGCCGCTTCTGCACCGGGTATTCttgtt                                                 <  2:2599702/93‑1 (MQ=255)
                                           gCTATGAGCGGTCCGTTACTGGCCGGGAAAATGCTGGCATTAGGCACCGGCACGGTCGGCGTAATGGCCGCTTCTGCACCGGGTATTCttgtt                                                 >  2:4099358/1‑93 (MQ=255)
                                           gCTATGAGCGGTCCGTTACTGGCCGGGAAAATGCTGGCATTAGGCACCGGCACGGTCGGCGTAATGGCCGCTTCTGCACCGGGTATTCttgtt                                                 <  2:1326856/93‑1 (MQ=255)
                                            cTATGAGCGGTCCGTGACTGGCCGGGAAAATGCTGGCATTAGGCACCGGCACGGTCGGCGTAATGGCCGCTTCTGCACCGTGTATTCttgttg                                                <  2:754488/93‑1 (MQ=255)
                                             tATGAGCGGTCCGTTACTGGCCGGGAAAATGCTGGCATTAGGCACCGGCACGGTCGGCGTAATGGCCGCTTCTGCACCGGGTATTCTTGTTGc                                               >  2:3828730/1‑93 (MQ=255)
                                             tATGAGCGGTCCGTTACTGGCCGGGAAAATGCTGGCATTAGGCACCGGCACGGTCGGCGTAATGGCCGCTTCTGCACCGGGTATTCTTGTTGc                                               >  1:815506/1‑93 (MQ=255)
                                             tATGAGCGGTCCGTTACTGGCCGGGAAAATGCTGGCATTAGGCACCGGCACGGTCGGCGTAATGGCCGCTTCTGCACCGGGTATTCTTGTTGc                                               >  1:2430988/1‑93 (MQ=255)
                                             tATGAGCGGTCCGTTACTGGCCGGGAAAATGCTGGCATTAGGCACCGGCACGGTCGGCGTAATGGCCGCTTCTGCACCGGGTATTCTTGGTGc                                               >  1:6384442/1‑93 (MQ=255)
                                             tATGAGCGGTCCGTTACTGGCCGGGAAAATGCTGGCATTAGGCACCGGCACGGTCAGCGTAATGGCCGCTTCTGCACCTGGTATTCTTGTTGc                                               >  1:4655418/1‑93 (MQ=255)
                                               tGAGCGGTCCGTTACTGGCCGGGAAAATGCTGGCATTAGGCACCGGCACGGTCGGCGTAATGGCCGCTTCTGCACCGGGTATTCTTGTTGCTg                                             >  1:6027606/1‑93 (MQ=255)
                                               tGAGCGGTCCGTTACTGGCCGGGAAAATGCTGGCATTAGGCACCGGCACGGTCGGCGTAATGGCCGCTTCTGCACCGGGTATTCTTGTTGCTg                                             <  2:46962/93‑1 (MQ=255)
                                               tGAGCGGTCCGTTACTGGCCGGGAAAATGCTGGCATTAGGCACCGGCACGGTCGGCGTAATGGCCGCTTCTGCACCGGGTATTCTTGTTGCTg                                             >  1:2563869/1‑93 (MQ=255)
                                               tGAGCGGTCCGTTACTGGCCGGGAAAATGCTGGCATTAGGCACCGGCACGGTCGGCGTAATGGCCGCTTCTGCACCGGGTATTCTTGTTGCTg                                             <  1:2621771/93‑1 (MQ=255)
                                                gAGCGGTCCGTTACTGGCCGGGAAAATGCTGGCATTAGGCACCGGCACGGTCGGCGTAATGGCCGCTTCTGCACCGTGTATTCTTGTTGCTgg                                            <  1:5400800/93‑1 (MQ=255)
                                                 aGCGGTCCGTTACTGGCCGGGAAAATGCTGGCATTAGGCACTGGCACGGTCGGCGTAATGGCCGCTTCTGCACCGGGTATTCTTGTTGCTggg                                           <  1:5364289/93‑1 (MQ=255)
                                                 aGCGGTCCGTTACTGGCCGGGAAAATGCTGGCATTAGGCACCGGCACGGTCGGCGTAATGGCCGCTTCTGCACCGGGTATTCTTGTTGCTggg                                           <  2:3076877/93‑1 (MQ=255)
                                                    ggTCCGTTACTGGCCGGGAAAATGCTGGCATTAGGCACCGGCACGGTCGGCGTAATGGCCGCTTCTGCACCGGGTATTCTTGTTGCTGGGTTg                                        <  1:1363137/93‑1 (MQ=255)
                                                       ccGTTACTGGCCGGGAAAATGCTGGCATTAGGCACCGGCACGGTCGGCGTAATGGCCGCTTCTGCACCGGGCATTCTTGTTGCTGGGTTGGCg                                     >  1:4308780/1‑93 (MQ=255)
                                                         gTTACTGGCCGGGAAAATGCTGGCATTAGGCACCGGCACGGTTGGCGTAATGGCCGCTTCTGCACCGGGTATTCTTGTTGCTGGGTTGGCggg                                   >  2:3459015/1‑92 (MQ=255)
                                                         gTTACTGGCCGGGAAAATGCTGGCATTAGGCACCGGCACGGTCGGCGTAATGGCCGCTTCTGCACCGGGTATTCTTGTTGCTGGGTTGGCggg                                   >  2:11188/1‑92 (MQ=255)
                                                         gTTACTGGCCGGGAAAATGCTGGCATTAGGCACCGGCACGGTCGGCGTAATGGCCGCTTCTGCACCGGGTATTCTTGTTGCTGGGTTGGCGgt                                   >  2:6058169/1‑93 (MQ=255)
                                                         gTTACTGGCCGGGAAAATGCTGGCATTAGGCACCGGCACGGTCGGCGTAATGGCCGCTTCTGCACCGGGTATTCTTGTTGCTGGGTTGGCGgt                                   >  2:838022/1‑93 (MQ=255)
                                                           tACTGGCCGGGAAAATGCTGGCATTAGGCACCGGCACGGTCGGCGTAATGGCCGCTTCTGCACCGGGTATTCTTGTTGCTGGGTTGGCGgtgt                                 >  1:2400375/1‑93 (MQ=255)
                                                           tACTGGCCGGGAAAATGCTGGCATTAGGCACCGGCACGGTCGGCGTAATGGCCGCTTCTGCACCGGGTATTCTTGTTGCTGGGTTGGCGgtgt                                 <  2:6097124/93‑1 (MQ=255)
                                                            tcTGGCCGGGAAAATGCTGGCATTAGGCACTGGCACGGTCGGCGTAATGGCCGCTTCTGCACCGGGTATTCTTGTTGCTGGGTTGGCGGTGtt                                <  1:4269744/92‑1 (MQ=255)
                                                            aCTGGCCGGGAAAATGCTGGCATTAGGCACCGGCACGGTCGGCGTAATGGCCGCTTCTGCACCGGGTATTCTTGTTGCTGGGTTGGCGGTGtt                                >  2:3563569/1‑93 (MQ=255)
                                                              tGGCCGGGAAAATGCTGGCATTAGGCACCGGCACGGTCGGCGTAATGGCCGCTTCTGCACCGGGTATTCTTGTTGCTGGGTTGGCGGTGtttt                              >  2:3424996/1‑92 (MQ=255)
                                                              tGGCCGGGAAAATGCTGGCATTAGGCACCGGCACGGTCGGCGTAATGGCCGCTTCTGCACCGGGTATTCTTGTTGCTGGGTTGGCGGTGTTTa                              <  1:2506577/93‑1 (MQ=255)
                                                                 ccGGGAAAATGCTGGCATTAGGCACCGGCACGGTCGGCGTAATGGCCGCTTCTGCACCGGGTATTCTTGTTGCTGGGTTGGCGGTGTTTAttt                           >  2:2321982/1‑93 (MQ=255)
                                                                  cGGGAAAATGCTGGCATTAGGCACCGGCACGGTCGGCGTAATGGCCGCTTCTGCACCGGGTATTCTTGTTGCTGGGTTGGCGGTGTTTAtttt                          >  2:5356012/1‑93 (MQ=255)
                                                                  cGGGAAAATGCTGGCATTAGGCACCGGCACGGTCGGCGTAATGGCCGCTTCTGCACCGGGTATTCTTGTTGCTGGGTTGGCGGTGTTTAtttt                          >  2:2895696/1‑93 (MQ=255)
                                                                  cGGGAAAATGCTGGCATTAGGCACCGGCACGGTCGGCGTAATGGCCGCTTCTGCACCGGGTATTCTTGTTGCTGGGTTGGCGGTGTTTAtttt                          >  1:3956410/1‑93 (MQ=255)
                                                                    ggAAAATGCTGGCATTAGGCACCGGCACGGTCGGCGTAATGGCCGCTTCTGCACCGGGTATTCTTGTTGCTGGGTTGGCGGTGTTTATTTtga                        <  2:5834327/93‑1 (MQ=255)
                                                                     gAAAATGCTGGCATTAGGCACCGGCACGGTCGGCGTAATGGCCGCTTCTGCACCGGGTATTCTTGTTGCTGGGTTGGCGGTGTTTATTTtgat                       <  2:3165105/93‑1 (MQ=255)
                                                                       aaaTGCTGGCATTAGGCACCGGCACGGTCGGCGTAATGGCCGCTTCTGCACCGGGTATTCTTGTTGCTGGGTTGGCGGTGTTTATTTtgatga                     <  2:4835406/93‑1 (MQ=255)
                                                                        aaTGCTGGCATTAGGCACCGGCACGGTCGGCGTAATGGCCGCTTCTGCACCGGGTATTCTTGTTGCTGGGTTGGCGGTGTTTATTTTGATGAg                    >  1:2140314/1‑93 (MQ=255)
                                                                        aaTGCTGGCATTAGGCACCGGCACGGTCGGCGTAATGGCCGCTTCTGCACCGGGTATTCTTGTTGCTGGGTTGGCGGTGTTTATTTTGATGAg                    <  1:1409592/93‑1 (MQ=255)
                                                                         aTGCTGGCATTAGGCACCGGCACGGTCGTCGTAATGGCCGCTTCTGCACCGGGTATTCTTGTTGCTGGGTTGGCGGTGTTTATTTTGATGAGc                   <  1:349586/93‑1 (MQ=255)
                                                                         aTGCTGGCATTAGGCACCGGCACGGTCGGCGTAATGGCCGCTTCTGCACCGGGTATTCTTGTTGCTGGGTTGGCGGTGTTTATTTTGATGAGc                   >  1:4493533/1‑93 (MQ=255)
                                                                         aTGCTGGCATTAGGCACCGGCACGGTCGGCGTAATGGCCGCTTCTGCACCGGGTATTCTTGTTGCTGGGTTGGCGGTGTTTATTTTGATGAGc                   >  1:1523264/1‑93 (MQ=255)
                                                                         aTGCTGGCATTAGGCACCGGCACGGTCGGCGTAATGGCCGCTTCTGCACCGGGTATTCTTGTTGCTGGGTTGGCGGTGTTTATTTTGATGAGc                   <  1:2092569/93‑1 (MQ=255)
                                                                          tGCTGGCATTAGGCACCGGCACGGTCGGCGTAATGGCCGCTTCTGCACCGGGTATTCTTGTTGCTGGGTTGGCGGTGTTTATTTTGATGAGcc                  >  1:3788250/1‑93 (MQ=255)
                                                                           gCTGGCATTAGGCACCGGCACGGTCGGCGTAATGGCCGCTTCTGCACCGGGTATTCTTGTTGCTGGGTTGGCGGTGTTTATTTTGATGAGCCg                 <  1:4772997/93‑1 (MQ=255)
                                                                            cTGGCATTAGGCACCGGCACGGTCGGCGTAATGGCCGCTTCTGCACCGGGTATTCTTGTTGCTGGGTTGGCGGTGTTTATTTTGATGAGCCgg                >  1:5384455/1‑93 (MQ=255)
                                                                               gCATTAGGCACCGGCACGGTCGGCGTAATGGCCGCTTCTGCACCGGGTATTCTTGTTGCTGGGTTGGCGGTGTTTATTTTGATGAGCCGgaga             >  1:236862/1‑93 (MQ=255)
                                                                               gCATTAGGCACCGGCACGGTCGGCGTAATGGCCGCTTCTGCACCGGGTATTCTTGTTGCTGGGTTGGCGGTGTTTATTTTGATGAGCCGgaga             <  1:2874284/93‑1 (MQ=255)
                                                                                cATTAGGCACCGGCACGGTCGGCGTAATGGCCGCTTCTGCACCGGGTATTCTTGTTGCTGGGTTGGCGGTGTTTATTTTGATGAGCCGGAGAt            <  1:3610585/93‑1 (MQ=255)
                                                                                cATTAGGCACCGGCACGGTCGGCGTAATGGCCGCTTCTGCACCGGGTATTCTTGTTGCTGGGTTGGCGGTGTTTATTTTGATGAGCCGGAGAt            <  2:1251232/93‑1 (MQ=255)
                                                                                cATTAGGCACCGGCACGGTCGGCGTAATGGCCGCTTCTGCACCGGGTATTCTTGTTGCTGGGTTGGCGGTGTTTATTTTGATGAGCCGGAGAt            <  1:2973709/93‑1 (MQ=255)
                                                                                 aTTAGGCGCCGGCACGGTCGGCGTAATGGCCGCTTCTGCACCGGGTATTCTTGTTGCTGGGTTGGCGGTGTTTATTTTGATGAGCCGGAGATc           <  1:1707446/93‑1 (MQ=255)
                                                                                 aTTAGGCACCGGCACGGTCGGCGTAATGGCCGCTTCTGCACCGGGTATTCTTGTTGCTGGGTTGGCGGTGTTTATTTTGATGAGCCGGAGATc           <  2:4746428/93‑1 (MQ=255)
                                                                                 aTTAGGCACCGGCACGGTCGGCGTAATGGCCGCTTCTGCACCGGGTATTCTTGTTGCTGGGTTGGCGGTGTTTATTTTGATGAGCCGGAGATc           >  1:735811/1‑93 (MQ=255)
                                                                                  ttAGGCACTGGCACGGTCGGCGTAATGGCCGCTTCTGCACCGGGTATTCTTGTTGCTGGGTTGGCGGTGTTTATTTTGATGAGCCGGAGATCa          <  2:2472146/93‑1 (MQ=255)
                                                                                  ttAGGCACCGGCACGGTCGGCGTAATGGCCGCTTCTGCACCGGGTATTCTTGTTGCTGGGTTGGCGGTGTTTATTTTGATGAGCCGGAGATCa          <  1:2534262/93‑1 (MQ=255)
                                                                                  ttAGGCACCGGCACGGTCGGCGTAATGGCCGCTTCTGCACCGGGTATTCTTGTTGCTGGGTTGGCGGTGTTTATTTTGATGAGCCGGAGATCa          >  1:518958/1‑93 (MQ=255)
                                                                                  ttAGGCACCGGCACGGTCGGCGTAATGGCCGCTTCTGCACCGGGTATTCTTGTTGCTGGGTTGGCGGTGTTTATTTTGATGAGCCGGAGATCa          >  1:1040150/1‑93 (MQ=255)
                                                                                  ttAGGCACCGGCACGGTCGGCGTAATGGCCGCTTCTGCACCGGGTATTCTTGTTGCTGGGTTGGCGGTGGTTATTTTGATGAGCCGGAGATCa          >  1:3403843/1‑93 (MQ=255)
                                                                                     ggCACCGGCACGGTCGGCGTAATGGCCGCTTCTGCACCGGGTATTCTTGTTGCTGGGTTGGCGGTGTTTATTTTGATGAGCCGGAGATCACGa       >  1:5711754/1‑93 (MQ=255)
                                                                                      gCACCGGCACGGTCGGCGTAATGGCCGCTTCTGCACCGGGTATTCTTGTTGCTGGGTTGGCGGTGTTTATTTTGATGAGCCGGAGATCACGaa      <  2:1343733/93‑1 (MQ=255)
                                                                                       cACCGGCACGGTCGGCGTAATGGCCGCTTCTGCACCGGGTATTCTTGTTGCTGGGTTGGCGGGGTTTATTTTTATGAGCCGGAGATCACGAAt     >  1:3543885/1‑93 (MQ=255)
                                                                                         cTGGCACGGTCGGCGTAAAGGCCGCTTCTGGACCGGGAACTCTTGCTGCTGGGTTAGCGATGCTTATTTTGATTAGCCGGAGATCACGAATAc   >  2:1008409/1‑93 (MQ=255)
                                                                                          tGGCACGGTCGGCGTAATGGCCGCTTCTGCACCGGGTATTCTTGTTGCTGGGTTGGCGGTGTTTATTTTGATGAGCCGGAGATCACGAATACa  <  1:3801839/93‑1 (MQ=255)
                                                                                          |                                                                                            
CGCAACAGGTGTGGGAACAGCCGTGGCGGTAGGGCGTCTGGGGGCTATGAGCGGTCCGTTACTGGCCGGGAAAATGCTGGCATTAGGCACTGGCACGGTCGGCGTAATGGCCGCTTCTGCACCGGGTATTCTTGTTGCTGGGTTGGCGGTGTTTATTTTGATGAGCCGGAGATCACGAATACA  >  NC_000913/375693‑375875

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 19 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
CGCGCAACAGGTGTGGGAACAGCCGTGGCGGTAGGGCGTCTGGGGGCTATGAGCGGTCCGTTACTGGCCGGGAAAATGCTGGCATTAGGCACTGGCACGGTCGGCGTAATGGCCGCTTCTGCACCGGGTATTCTTGTTGCTGGGTTGGCGGTGTTTATTTTGATGAGCCGGAGATCACGAATACAG  >  NC_000913/375691‑375876
                                                                                            |                                                                                             
CGCGCAACAGGTGTGGGAACAGCCGTGGCGGTAGGGCGTCTGGGGGCTATGAGCGGTCCGTTACTGGCCGGGAAAATGCTGGCATTAGGCACCG                                                                                              <  SRR4302537.6151163/94‑1 (MQ=60)
       CAGGTGTGGGAACAGCCGTGGCGGTAGGGCGTCTGGGGGCTATGAGCGGTCCGTTACTGGCCGGGAAAATGCTGGCATTAGGCACCGGCACGGT                                                                                       <  SRR4302537.3422413/94‑1 (MQ=60)
       CAGGTGTGGGAACAGCCGTGGCGGTAGGGCGTCTGGGGGCTATGAGCGGTCCGTTACTGGCCGGGAAAATGCTGGCATTAGGCACCGGCACGGT                                                                                       <  SRR4302537.2262402/94‑1 (MQ=60)
       CAGGTGTGGGAACAGCCGTGGCGGTAGGGCGTCTGGGGGCTATGAGCGGTCCGTTACTGGCCGGGAAAATGCTGGCATTAGGCACCGGCACGGT                                                                                       <  SRR4302537.2256845/94‑1 (MQ=60)
        AGGTGTGGGAACAGCCGTGGCGGTAGGGCGTCTGGGGGCTATGAGCGGTCCGTTACTGGCCGGGAAAATGCTGGCATTAGGCACTGGAACGGTG                                                                                      >  SRR4302537.1195261/1‑94 (MQ=60)
        AGGTGTGGGAACAGCCGTGGCGGTAGGGCGTCTGGGGGCTATGAGCGGTCCGTTACTGGCCGGGAAAATGCTGGCATTAGGCACCGGCACGGTC                                                                                      >  SRR4302537.3151130/1‑94 (MQ=60)
        AGGTGTGGGAACAGCCGTGGCGGTAGGGCGTCTGGGGGCTATGAGCGGTCCGTTACTGGCCGGGAAAATGCTGGCATTAGGCACCGGCACGGTC                                                                                      <  SRR4302537.5873333/94‑1 (MQ=60)
             TGGGAACAGCCGTGGCGGTAGGGCGTCTGGGGGCTATGAGCGGTCCGTTACTGGCCGGGAAAATGCTGGCATTAGGCACCGGCACGGTCGGCGT                                                                                 >  SRR4302537.1447920/1‑94 (MQ=60)
                GAACAGCCGTGGCGGTAGGGCGTCTGGGGGCTATGAGCGGTCCGTTACTGGCCGGGAAAATGCTGGCATTAGGCACCGGCACGGTCGGCGTAAT                                                                              <  SRR4302537.3630103/94‑1 (MQ=60)
                GAACAGCCGTGGCGGTAGGGCGTCTGGGGGCTATGAGCGGTCCGTTACTGGCCGGGAAAATGCTGGCATTAGGCACCGGCACGGTCGGCGTAAT                                                                              <  SRR4302537.653834/94‑1 (MQ=60)
                 AACAGCCGTGGCGGTAGGGCGTCTGGGGGCTATGAGCGGTCCGTTACTGGCCGGGAAAATGCTGGCATTAGGCACCGGCACGGTCGGCGTAATG                                                                             <  SRR4302537.2923617/94‑1 (MQ=60)
                    AGCCGTGGCGGTAGGGCGTCTGGGGGCTATGAGCGGTCCGTTACTGGCCGGGAAAATGCTGGCATTAGGCACCGGCACGGTCGGCGTAATGGCC                                                                          <  SRR4302537.4682158/94‑1 (MQ=60)
                       CGTGGCGGTAGGGCGTCTGGGGGCTATGAGCGGTCCGTTACTGGCCGGGAAAATGCTGGCATTAGGCACCGGCACGGTCGGCGTAATGGCCGCT                                                                       >  SRR4302537.4056483/1‑94 (MQ=60)
                       CGTGGCGGTAGGGCGTCTGGGGGCTATGAGCGGTCCGTTACTGGCCGGGAAAATGCTGGCATTAGGCACCGGCACGGTCGGCGTAATGGCCGCT                                                                       <  SRR4302537.5367664/94‑1 (MQ=60)
                       CGTGGCGGTAGGGCGTCTGGGGGCTATGAGCGGTCCGTTACTGGCCGGGAAAATGCTGGCATTAGGCACCGGCACGGTCGGCGTAATGGCCGCT                                                                       <  SRR4302537.1010390/94‑1 (MQ=60)
                        GTGGCGGTAGGGCGTCTGGGGGCTATGAGCGGTCCGTTACTGGCCGGGAAAATGCTGGCATTAGGCACCGGCACGGTCGGCGTAATGGCCGCTT                                                                      <  SRR4302537.5497869/94‑1 (MQ=60)
                                 GGGCGTCTGGGGGCTATGAGCGGTCCGTTACTGGCCGGGAAAATGCTGGCATTAGGCACCGGCACGGTCGGCGTAATGGCCGCTTCTGCACCGG                                                             <  SRR4302537.4758739/94‑1 (MQ=60)
                                 GGGCGTCTGGGGGCTATGAGCGGTCCGTTACTGGCCGGGAAAATGCTGGCATTAGGCACCGGCACGGTCGGCGTAATGGCCGCTTCTGCACCGG                                                             >  SRR4302537.1984681/1‑94 (MQ=60)
                                     GTCTGGGGGCTATGAGCGGTCCGTTACTGGCCGTGAAAATGCTGGCATTAGGCACCGGCACGGTCGGCGTAATGGCCGCTTCTGCACCGGGTAT                                                         <  SRR4302537.4672646/94‑1 (MQ=60)
                                     GTCTGGGGGCTATGAGCGGTCCGTTACTGGCCGGGAAAATGCTGGCATTAGGCACCGGCACGGTCGGCGTAATGGCCGCTTCTGCACCGGGTAT                                                         >  SRR4302537.2445400/1‑94 (MQ=60)
                                         GGGGGCTATGAGCGGTCCGTTACTGGCCGGGAAAATGCTGGCATTAGGCACCGGCACGGTCGGCGTAATGGCCGCTTCTGCACCGGGTATTCTT                                                     <  SRR4302537.2605859/94‑1 (MQ=60)
                                         GGGGGCTATGAGCGGTCCGTTACTGGCCGGGAAAATGCTGGCATTAGGCACCGGCACGGTCGGCGTAATGGCCGCTTCTGCACCGGGTATTCTT                                                     >  SRR4302537.2529668/1‑94 (MQ=60)
                                            GGCTATGAGCGGTCCGTTACTGGCCGGGAAAATGCTGGCATTAGGCACCGGCACGGTCGGCGTAATGGCCGCTTCTGCACCGGGTATTCTTGTT                                                  <  SRR4302537.287964/94‑1 (MQ=60)
                                            GGCTATGAGCGGTCCGTTACTGGCCGGGAAAATGCTGGCATTAGGCACCGGCACGGTCGGCGTAATGGCCGCTTCTGCACCGGGTATTCTTGTT                                                  <  SRR4302537.2925790/94‑1 (MQ=60)
                                             GCTATGAGCGGTCCGTTACTGGCCGGGAAAATGCTGGCATTAGGCACCGGCACGGTCGGCGTAATGGCCGCTTCTGCACCGGGTATTCTTGTTG                                                 >  SRR4302537.161606/1‑94 (MQ=60)
                                              CTATGAGCGGTCCGTTACTGGCCGGGAAAATGCTGGCATTAGGCACCGGCACGGTCGGCGTAATGGCCGCTTCTGCACCGGGTATTCTTGTTGC                                                >  SRR4302537.2448114/1‑94 (MQ=60)
                                              CTATGAGCGGTCCGTTACTGGCCGGGAAAATGCTGGCATTAGGCACCGGCACGGTCGGCGTAATGGCCGCTTCTGCACCGGGTATTCTTGTTGC                                                >  SRR4302537.821109/1‑94 (MQ=60)
                                              CTATGAGCGGTCCGTTACTGGCCGGGAAAATGCTGGCATTAGGCACCGGCACGGTCGGCGTAATGGCCGCTTCTGCACCGGGTATTCTTGGTGC                                                >  SRR4302537.6427958/1‑94 (MQ=60)
                                              CTATGAGCGGTCCGTTACTGGCCGGGAAAATGCTGGCATTAGGCACCGGCACGGTCAGCGTAATGGCCGCTTCTGCACCTGGTATTCTTGTTGC                                                >  SRR4302537.4687606/1‑94 (MQ=60)
                                               TATGAGCGGTCCGTTACTGGCCGGGAAAATGCTGGCATTAGGCACCGGCACGGTCGGCGTAATGGCCGCTTCTGCACCGGGTATTCTTGTTGCT                                               >  SRR4302537.5907527/1‑94 (MQ=60)
                                               TATGAGCGGTCCGTTACTGGCCGGGAAAATGCTGGCATTAGGCACCGGCACGGTCGGCGTAATGGCCGCTTCTGCACCGGGTATTCTTGTTGCT                                               >  SRR4302537.2130854/1‑94 (MQ=60)
                                                ATGAGCGGTCCGTTACTGGCCGGGAAAATGCTGGCATTAGGCACCGGCACGGTCGGCGTAATGGCCGCTTCTGCACCGGGTATTCTTGTTGCTG                                              >  SRR4302537.3704208/1‑94 (MQ=60)
                                                ATGAGCGGTCCGTTACTGGCCGGGAAAATGCTGGCATTAGGCACCGGCACGGTCGGCGTAATGGCCGCTTCTGCACCGGGTATTCTTGTTGCTG                                              >  SRR4302537.2581832/1‑94 (MQ=60)
                                                ATGAGCGGTCCGTTACTGGCCGGGAAAATGCTGGCATTAGGCACCGGCACGGTCGGCGTAATGGCCGCTTCTGCACCGGGTATTCTTGTTGCTG                                              >  SRR4302537.6069024/1‑94 (MQ=60)
                                                 TGAGCGGTCCGTTACTGGCCGGGAAAATGCTGGCATTAGGCACCGGCACGGTCGGCGTAATGGCCGCTTCTGCACCGGGTATTCTTGTTGCTGG                                             <  SRR4302537.2640138/94‑1 (MQ=60)
                                                  GAGCGGTCCGTTACTGGCCGGGAAAATGCTGGCATTAGGCACCGGCACGGTCGGCGTAATGGCCGCTTCTGCACCGTGTATTCTTGTTGCTGGG                                            <  SRR4302537.5437663/94‑1 (MQ=60)
                                                  GAGCGGTCCGTTACTGGCCGGGAAAATGCTGGCATTAGGCACCGGCACGGTCGGCGTAATGGCCGCTTCTGCACCGGGTATTCTTGTTGCTGGG                                            <  SRR4302537.1411048/94‑1 (MQ=60)
                                                   AGCGGTCCGTTACTGGCCGGGAAAATGCTGGCATTAGGCACTGGCACGGTCGGCGTAATGGCCGCTTCTGCACCGGGTATTCTTGTTGCTGGGT                                           <  SRR4302537.5401005/94‑1 (MQ=60)
                                                      GGTCCGTTACTGGCCGGGAAAATGCTGGCATTAGGCACCGGCACGGTCGGCGTAATGGCCGCTTCTGCACCGGGTATTCTTGTTGCTGGGTTGG                                        <  SRR4302537.5285463/94‑1 (MQ=60)
                                                      GGTCCGTTACTGGCCGGGAAAATGCTGGCATTAGGCACCGGCACGGTCGGCGTAATGGCCGCTTCTGCACCGGGTATTCTTGTTGCTGGGTTGG                                        >  SRR4302537.824291/1‑94 (MQ=60)
                                                      GGTCCGTTACTGGCCGGGAAAATGCTGGCATTAGGCACCGGCACGGTCGGCGTAATGGCCGCTTCTGCACCGGGTATTCTTGTTGCTGGGTTGG                                        <  SRR4302537.1372632/94‑1 (MQ=60)
                                                        TCCGTTACTGGCCGGGAAAATGCTGGCATTAGGCACCGGCACGGTCGGCGTAATGGCCGCTTCTGCACCGGGCATTCTTGTTGCTGGGTTGGCG                                      >  SRR4302537.4338729/1‑94 (MQ=60)
                                                           GTTACTGGCCGGGAAAATGCTGGCATTAGGCACCGGCACGGTCGGCGTAATGGCCGCTTCTGCACCGGGTATTCTTGTTGCTGGGTTGGCGGTG                                   >  SRR4302537.1522723/1‑94 (MQ=60)
                                                            TTACTGGCCGGGAAAATGCTGGCATTAGGCACCGGCACGGTCGGCGTAATGGCCGCTTCTGCACCGGGTATTCTTGTTGCTGGGTTGGCGGTGT                                  >  SRR4302537.2417281/1‑94 (MQ=60)
                                                            TTACTGGCCGGGAAAATGCTGGCATTAGGCACCGGCACGGTCGGCGTAATGGCCGCTTCTGCACCGGGTATTCTTGTTGCTGGGTTGGCGGTGT                                  >  SRR4302537.3837411/1‑94 (MQ=60)
                                                              TCTGGCCGGGAAAATGCTGGCATTAGGCACTGGCACGGTCGGCGTAATGGCCGCTTCTGCACCGGGTATTCTTGTTGCTGGGTTGGCGGTGTTT                                <  SRR4302537.4299410/94‑1 (MQ=60)
                                                                TGGCCGGGAAAATGCTGGCATTAGGCACCGGCACGGTCGGCGTAATGGCCGCTTCTGCACCGGGTATTCTTGTTGCTGGGTTGGCGGTGTTTAT                              <  SRR4302537.2524208/94‑1 (MQ=60)
                                                                   CCGGGAAAATGCTGGCATTAGGCACCGGCACGGTCGGCGTAATGGCCGCTTCTGCACCGGGTATTCTTGTTGCTGGGTTGGCGGTGTTTATTTT                           >  SRR4302537.3984094/1‑94 (MQ=60)
                                                                       GAAAATGCTGGCATTAGGCACCGGCACGGTCGGCGTAATGGCCGCTTCTGCACCGGGTATTCTTGTTGCTGGGTTGGCGGTGTTTATTTTGATG                       >  SRR4302537.3021812/1‑94 (MQ=60)
                                                                         AAATGCTGGCATTAGGCACCGGCACGGTCGGCGTAATGGCCGCTTCTGCACCGGGTATTCTTGTTGCTGGGTTGGCGGTGTTTATTTTGATGAG                     >  SRR4302537.2155378/1‑94 (MQ=60)
                                                                         AAATGCTGGCATTAGGCACCGGCACGGTCGGCGTAATGGCCGCTTCTGCACCGGGTATTCTTGTTGCTGGGTTGGCGGTGTTTATTTTGATGAG                     <  SRR4302537.805187/94‑1 (MQ=60)
                                                                          AATGCTGGCATTAGGCACCGGCACGGTCGGCGTAATGGCCGCTTCTGCACCGGGTATTCTTGTTGCTGGGTTGGCGGTGTTTATTTTGATGAGC                    >  SRR4302537.1533906/1‑94 (MQ=60)
                                                                          AATGCTGGCATTAGGCACCGGCACGGTCGGCGTAATGGCCGCTTCTGCACCGGGTATTCTTGTTGCTGGGTTGGCGGTGTTTATTTTGATGAGC                    <  SRR4302537.1419411/94‑1 (MQ=60)
                                                                          AATGCTGGCATTAGGCACCGGCACGGTCGGCGTAATGGCCGCTTCTGCACCGGGTATTCTTGTTGCTGGGTTGGCGGTGTTTATTTTGATGAGC                    >  SRR4302537.4524620/1‑94 (MQ=60)
                                                                           ATGCTGGCATTAGGCACCGGCACGGTCGTCGTAATGGCCGCTTCTGCACCGGGTATTCTTGTTGCTGGGTTGGCGGTGTTTATTTTGATGAGCC                   <  SRR4302537.352019/94‑1 (MQ=60)
                                                                           ATGCTGGCATTAGGCACCGGCACGGTCGGCGTAATGGCCGCTTCTGCACCGGGTATTCTTGTTGCTGGGTTGGCGGTGTTTATTTTGATGAGCC                   <  SRR4302537.4304311/94‑1 (MQ=60)
                                                                           ATGCTGGCATTAGGCACCGGCACGGTCGGCGTAATGGCCGCTTCTGCACCGGGTATTCTTGTTGCTGGGTTGGCGGTGTTTATTTTGATGAGCC                   >  SRR4302537.3814844/1‑94 (MQ=60)
                                                                           ATGCTGGCATTAGGCACCGGCACGGTCGGCGTAATGGCCGCTTCTGCACCGGGTATTCTTGTTGCTGGGTTGGCGGTGTTTATTTTGATGAGCC                   <  SRR4302537.2107327/94‑1 (MQ=60)
                                                                           ATGCTGGCATTAGGCACCGGCACGGTCGGCGTAATGGCCGCTTCTGCACCGGGTATTCTTGTTGCTGGGTTGGCGGTGTTTATTTTGATGAGCC                   <  SRR4302537.3464798/94‑1 (MQ=60)
                                                                             GCTGGCATTAGGCACCGGCACGGTCGGCGTAATGGCCGCTTCTGCACCGGGTATTCTTGTTGCTGGGTTGGCGGTGTTTATTTTGATGAGCCGG                 <  SRR4302537.4805951/94‑1 (MQ=60)
                                                                             GCTGGCATTAGGCACCGGCACGGTCGGCGTAATGGCCGCTTCTGCACCGGGTATTCTTGTTGCTGGGTTGGCGGTGTTTATTTTGATGAGCCGG                 >  SRR4302537.5420177/1‑94 (MQ=60)
                                                                             GCTGGCATTAGGCACCGGCACGGTCGGCGTAATGGCCGCTTCTGCACCGGGTATTCTTGTTGCTGGGTTGGCGGTGTTTATTTTGATGAGCCGG                 >  SRR4302537.5421267/1‑94 (MQ=60)
                                                                                GGCATTAGGCACCGGCACGGTCGGCGTAATGGCCGCTTCTGCACCGGGTATTCTTGTTGCTGGGTTGGCGGTGTTTATTTTGATGAGCCGGAGA              <  SRR4302537.179219/94‑1 (MQ=60)
                                                                                GGCATTAGGCACCGGCACGGTCGGCGTAATGGCCGCTTCTGCACCGGGTATTCTTGTTGCTGGGTTGGCGGTGTTTATTTTGATGAGCCGGAGA              >  SRR4302537.238531/1‑94 (MQ=60)
                                                                                 GCATTAGGCACCGGCACGGTCGGCGTAATGGCCGCTTCTGCACCGGGTATTCTTGTTGCTGGGTTGGCGGTGTTTATTTTGATGAGCCGGAGAT             <  SRR4302537.2894455/94‑1 (MQ=60)
                                                                                  CATTAGGCACCGGCACGGTCGGCGTAATGGCCGCTTCTGCACCGGGTATTCTTGTTGCTGGGTTGGCGGTGTTTATTTTGATGAGCCGGAGATC            >  SRR4302537.740829/1‑94 (MQ=60)
                                                                                  CATTAGGCACCGGCACGGTCGGCGTAATGGCCGCTTCTGCACCGGGTATTCTTGTTGCTGGGTTGGCGGTGTTTATTTTGATGAGCCGGAGATC            <  SRR4302537.3636037/94‑1 (MQ=60)
                                                                                  CATTAGGCACCGGCACGGTCGGCGTAATGGCCGCTTCTGCACCGGGTATTCTTGTTGCTGGGTTGGCGGTGTTTATTTTGATGAGCCGGAGATC            <  SRR4302537.2994536/94‑1 (MQ=60)
                                                                                   ATTAGGCGCCGGCACGGTCGGCGTAATGGCCGCTTCTGCACCGGGTATTCTTGTTGCTGGGTTGGCGGTGTTTATTTTGATGAGCCGGAGATCA           <  SRR4302537.1719380/94‑1 (MQ=60)
                                                                                   ATTAGGCACCGGCACGGTCGGCGTAATGGCCGCTTCTGCACCGGGTATTCTTGTTGCTGGGTTGGCGGTGTTTATTTTGATGAGCCGGAGATCA           >  SRR4302537.1047309/1‑94 (MQ=60)
                                                                                   ATTAGGCACCGGCACGGTCGGCGTAATGGCCGCTTCTGCACCGGGTATTCTTGTTGCTGGGTTGGCGGTGTTTATTTTGATGAGCCGGAGATCA           <  SRR4302537.5389386/94‑1 (MQ=60)
                                                                                   ATTAGGCACCGGCACGGTCGGCGTAATGGCCGCTTCTGCACCGGGTATTCTTGTTGCTGGGTTGGCGGTGTTTATTTTGATGAGCCGGAGATCA           >  SRR4302537.522529/1‑94 (MQ=60)
                                                                                   ATTAGGCACCGGCACGGTCGGCGTAATGGCCGCTTCTGCACCGGGTATTCTTGTTGCTGGGTTGGCGGTGTTTATTTTGATGAGCCGGAGATCA           >  SRR4302537.4625625/1‑94 (MQ=60)
                                                                                   ATTAGGCACCGGCACGGTCGGCGTAATGGCCGCTTCTGCACCGGGTATTCTTGTTGCTGGGTTGGCGGTGGTTATTTTGATGAGCCGGAGATCA           >  SRR4302537.3427952/1‑94 (MQ=60)
                                                                                    TTAGGCACCGGCACGGTCGGCGTAATGGCCGCTTCTGCACCGGGTATTCTTGTTGCTGGGTTGGCGGTGTTTATTTTGATGAGCCGGAGATCAC          <  SRR4302537.2552047/94‑1 (MQ=60)
                                                                                     TAGGCACCGGCACGGTCGGCGTAATGGCCGCTTCTGCACCGGGTATTCTTGTTGCTGGGTTGGCGGTGTTTATTTTGATGAGCCGGAGATCACG         <  SRR4302537.5816971/94‑1 (MQ=60)
                                                                                     TAGGCACCGGCACGGTCGGCGTAATGGCCGCTTCTGCACCGGGTATTCTTGTTGCTGGGTTGGCGGAGTTTATTTTGATGAGACGGAGATCACG         >  SRR4302537.2506032/1‑94 (MQ=60)
                                                                                      AGGCACCGGCACGGTCGGCGTAATGGCCGCTTCTGCACCGGGTATTCTTGTTGCTGGGTTGGCGGTGTTTATTTTGATGAGCCGGAGATCACGA        >  SRR4302537.5750849/1‑94 (MQ=60)
                                                                                        GCACCGGCACGGTCGGCGTAATGGCCGCTTCTGCACCGGGTATTCTTGTTGCTGGGTTGGCGGGGTTTATTTTTATGAGCCGGAGATCACGAAT      >  SRR4302537.3568919/1‑94 (MQ=60)
                                                                                         CACCGGCACGGTCGGCGTAATGGCCGCTTCTGCACCGGGTATTCTTGTTGCTGGGTTGGCGGTGTTTATTTTGATGAGCCGGAGATCACGAATA     <  SRR4302537.2232780/94‑1 (MQ=60)
                                                                                           CCGGCACGGTCGGCGTAATGGCCGCTTCTGCACCGGGTATTCTTGTTGCTGGGTTGGCGGTGTTTATTTTGATGAGCCGGAGATCACGAATACA   <  SRR4302537.1496431/94‑1 (MQ=60)
                                                                                            TGGCACGGTCGGCGTAATGGCCGCTTCTGCACCGGGTATTCTTGTTGCTGGGTTGGCGGTGTTTATTTTGATGAGCCGGAGATCACGAATACAG  <  SRR4302537.3828510/94‑1 (MQ=60)
                                                                                            CGGCACGGTCGGCGTAATGGCCGCTTCTGCACCGGGTATTCTTGTTGCTGGGTTGGCGGTGTTTATTTTGATGAGCCGGAGATCACGAATACAG  <  SRR4302537.2613471/94‑1 (MQ=60)
                                                                                            CGGCACGGTCGGCGTAATGGCCGCTTCTGCACCGGGTATTCTTGTTGCTGGGTTGGCGGTGTTTATTTTGATGAGCCGGAGATCACGAATACAG  <  SRR4302537.5831881/94‑1 (MQ=60)
                                                                                            CGGCACGGTCGGCGTAATGGCCGCTTCTGCACCGGGTATTCTTGTTGCTGGGTTGGCGGTGTTTATTTTGATGAGCCGGAGATCACGAATACAG  <  SRR4302537.4184329/94‑1 (MQ=60)
                                                                                            |                                                                                             
CGCGCAACAGGTGTGGGAACAGCCGTGGCGGTAGGGCGTCTGGGGGCTATGAGCGGTCCGTTACTGGCCGGGAAAATGCTGGCATTAGGCACTGGCACGGTCGGCGTAATGGCCGCTTCTGCACCGGGTATTCTTGTTGCTGGGTTGGCGGTGTTTATTTTGATGAGCCGGAGATCACGAATACAG  >  NC_000913/375691‑375876

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 17 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑