Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A1 F1 I2 R1	882	254.4	12957588	98.0%	12698436	93.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	435,408	A→G	D184G (GAC→GGC)	thiL →	thiamin‑monophosphate kinase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	435,408	0	A	G	94.7%	640.5 / 16.3	208	D184G (GAC→GGC)	thiL	thiamin‑monophosphate kinase
Reads supporting (aligned to +/- strand): ref base A (7/4); new base G (91/106); total (98/110)
Fisher's exact test for biased strand distribution p-value = 3.55e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.15e-01

Only 100 of 225 total aligned reads displayed.

GGATCTATGTGACCGGTACACCGGGCGATAGCGCCGCCGGGCTGGCGATTTTGCAAAACCGTTTGCAGGTTGCCGATGCTAAAGATGCGGACTACTTGATCAAACGTCATCTCCGTCCATCGCCGCGTATTTTACAGGGGCAGGCACTGCGCGATCTGGCAAATTCAGCCATCGATCTCTC  >  NC_000913/435318‑435498

ggATCTATGTGACCGGTACACCGGGCGATAGCGCCGCCGGGCTGGCGATTTTGCAAAACCGTTTGCAGGTTGCCGATGCTAAAGATGCGGgct                                                                                          <  1:413708/93‑1 (MQ=255)

ggATCTATGTGACCGGTACACCGGGCGATAGCGCCGCCGGGCTGGCGATTTTGCAAAACCGTTTGCAGGTTGCCGATGCTAAAGATGCGGgct                                                                                          <  1:3233277/93‑1 (MQ=255)

  aTCTATGTGACCGGTACACCGGGCGATAGCGCCGCCGGGCTGGCGATTTTGCAAAACCGTTTGCAGGTTGCCGATGCTAAAGATGCGGgctac                                                                                        >  2:36051/1‑93 (MQ=255)

    cTATGTGACCGGTACACCGGGCGATAGCGCCGCCGGGCTGGCGATTTTGCAAAACCGTTTGCAGGTTGCCGATGCTAAAGATGCGGGCTACtt                                                                                      <  1:1925985/93‑1 (MQ=255)

      aTGTTACCGGTACACCGGGCGATAGCGCCGCCGGGCTGGCGATTTTGCAAAACCGTTTGCAGGTTGCCGATGCTAAAGATGCGGGCTACTTGa                                                                                    >  2:141606/1‑93 (MQ=255)

      aTGTGACCGGTACACCGGGCGATAGCGCCGCCGGGCTGGCGATTTTGCAAAACCGTTTGCAGGTTGCCGATGCTAAAGATGCGGGCTACTTGa                                                                                    >  1:3344875/1‑93 (MQ=255)

      aTGTGACCGGTACACCGGGCGATAGCGCCGCCGGGCTGGCGATTTTGCAAAACCGTTTGCAGGTTGCCGATGCTAAAGATGCGGGCTACTTGa                                                                                    >  1:6204756/1‑93 (MQ=255)

      aTGTGACCGGTACACCGGGCGATAGCGCCGCCGGGCTGGCGATTTTGCAAAACCGTTTGCAGGTTGCCGATGCTAAAGATGCGGGCTACTTGa                                                                                    <  2:1138178/93‑1 (MQ=255)

      aTGTGACCGGTACACCGGGCGATAGCGCCGCCGGGCTGGCGATTTTGCAAAACCGTTTGCAGGTTGCCGATGCTAAAGATGCGGGCTACTTGa                                                                                    <  2:4857965/93‑1 (MQ=255)

       tgtgACCGGTACACCGGGCGATAGCGCCGCCGGGCTGGCGATTTTGCAAAACCGTTTGCAGGTTGCCGATGCTAAAGATGCGGGCTACTTGAt                                                                                   <  1:526135/93‑1 (MQ=255)

       tgtgACCGGTACACCGGGCGATAGCGCCGCCGGGCTGGCGATTTTGCAAAACCGTTTGCAGGTTGCCGATGCTAAAGATGCGGGCTACTTGAt                                                                                   <  2:3183832/93‑1 (MQ=255)

       tgtgACCGGTACACCGGGCGATAGCGCCGCCGGGCTGGCGATTTTGCAAAACCGTTTGCAGGTTGCCGATGCTAAAGATGCGGGCTACTTGAt                                                                                   >  1:815009/1‑93 (MQ=255)

       tgtgACCGGTACACCGGGCGATAGCGCCGCCGGGCTGGCGATTTTGCAAAACCGTTTGCAGGTTGCCGATGCTAAAGATGCGGGCTACTTGAt                                                                                   <  1:6432370/93‑1 (MQ=255)

        gtgACCGGTACACCGGGCGATAGCGCCGCCGGGCTGGCGATTTTGCAAAACCGTTTGCAGGTTGCCGATGCTAAAGATGCGGGCTACTTGATc                                                                                  <  1:5125820/93‑1 (MQ=255)

         tgACCGGTACACCGGGCGATAGCGCCGCCGGGCTGGCGATTTTGCAAAACCGTTTGCAGGTTGCCGATGCTAAAGATGCGGGCTACTTGATCa                                                                                 >  2:6429212/1‑93 (MQ=255)

           aCCGGTACACCGGGCGATAGCGCCGCCGGGCTGGCGATTTTGCAAAACCGTTTGCAGGTTGCCGATGCTAAAGATGCGGGCTACTTGATCaaa                                                                               >  2:2959151/1‑93 (MQ=255)

            ccGGTACTCCGGGCGATAGCGCCGCCGGGCTGCCGATTTTGCAAAACCGTTTGCAGGTTGCCGATGCTAAAGATGCGGGCTACTTGATCAAAc                                                                              <  2:3382746/93‑1 (MQ=255)

            ccGGTACACCGGGCGATAGCGCCGCCGGGCTGGCGATTTTGCAAAACCGTTTGCAGGTTGCCGATGCTAAAGATGCGGGCTACTTGATCAAAc                                                                              >  1:6077329/1‑93 (MQ=255)

            ccGGTACACCGGGCGATAGCGCCGCCGGGCTGGCGATTTTGCAAAACCGTTTGCAGGTTGCCGATGCTAAAGATGCGGGCTACTTGATCAAAc                                                                              >  2:2155690/1‑93 (MQ=255)

             cGGTACACCGGGCGATAGCGCCGCCGGGCTGGCGATTTTGCAAAACCGTTTGCAGGTTGCCGTTGCTAAAGATGCGGGCTCCTTGAGCAAACg                                                                             >  1:4980514/1‑93 (MQ=255)

              ggTACACCGGGCGATAGCGCCGCCGGGCTGGCGATTTTGCAAAACCGTTTGCAGGTTGCCGATGCTAAAGATGCGGGCTACTTGATCAAACGt                                                                            <  2:2042464/93‑1 (MQ=255)

               gTACACCGGGCGATAGCGCCGCCGGGCTGGCGATTTTGCAAAACCGTTTGCAGGTTGCCGATGCTAAAGATGCGGGCTACTTGATCAAACGTc                                                                           >  2:1333757/1‑93 (MQ=255)

               gTACACCGGGCGATAGCGCCGCCGGGCTGGCGATTTTGCAAAACCGTTTGCAGGTTGCCGATGCTAAAGATGCGGGCTACTTGATCAAACGTc                                                                           <  1:5831021/93‑1 (MQ=255)

               gTACACCGGGCGATAGCGCCGCCGGGCTGGCGATTTTGCAAAACCGTTTGCAGGTTGCCGATGCTAAAGATGCGGGCTACTTGATCAAACGTc                                                                           <  2:3946321/93‑1 (MQ=255)

                tACACCGGGCGATAGCGCCGCCGGGCTGGCGATTTTGCAAAACCGTTTGCAGGTTGCCGATGCTAAAGATGCGGGCTACTTGATCAAACGTCa                                                                          <  2:3554056/93‑1 (MQ=255)

                  cacCGGGCGATAGCGCCGCCGGGCTGGCGATTTTGCAAAACCGTTTGCAGGTTGCCGATGCTAAAGATGCGGGCTACTTGATCAAACGTCAtc                                                                        <  2:6272687/93‑1 (MQ=255)

                    ccGGGCGATAGCGCCGCCGGGCTGGCGATTTTGCAAAACCGTTTGCAGGTTGCCGATGCTAAAGATGCGGGCTACTTGATCAAACGTCAtctc                                                                      <  1:6380840/93‑1 (MQ=255)

                    ccGGGCGATAGCGCCGCCGGGCTGGCGATTTTGCAAAACCGTTTGCAGGTTGCCGATGCTAAAGATGCGGGCTACTTGATCAAACGTCAtctc                                                                      <  1:4888224/93‑1 (MQ=255)

                     cGGGCGATAGCGCCGCCGGGCTGGCGATTTTGCAAAACCGTTTGCAGGTTGCCGATGCTAAAGATGCGGGCTACTTGATCAAACGTCATCTcc                                                                     <  1:1574778/93‑1 (MQ=255)

                        gCGATAGCGCCGCCGGGCTGGCGATTTTGCAAAACCGTTTGCAGGTTGCCGATGCTAAAGATGCGGGCTACTTGATCAAACGTCATCTCCGTc                                                                  <  1:254139/93‑1 (MQ=255)

                         cGATAGCGCCGCCGGGCTGGCGATTTTGCAAAACCGTTTGCAGGTTGCCGATGCTAAAGATGCGGGCTACTTGATCAAACGTCATCTCCGTcc                                                                 <  1:4922335/93‑1 (MQ=255)

                           aTAGCGCCGCCGGGCTGGCGATTTTGCAAAACCGTTTGCAGGTTGCCGATGCTAAAGATGCGGGCTACTTGATCAAACGTCATCTCCGTCCAt                                                               >  1:4617333/1‑93 (MQ=255)

                           aTAGCGCCGCCGGGCTGGCGATTTTGCAAAACCGTTTGCAGGTTGCCGATGCTAAAGATGCGGGCTACTTGATCAAACGTCATCTCCGTCCAt                                                               >  2:6080931/1‑93 (MQ=255)

                           aTAGCGCCGCCGGGCTGGCGATTTTGCAAAACCGTTTGCAGGTTGCCGATGCTAAAGATGCGGGCTACTTGATCAAACGTCATCTCCGTCCAt                                                               >  1:2259484/1‑93 (MQ=255)

                            tAGCGCCGCCGGGCTGGCGATTTTGCAAAACCGTTTGCAGGTTGCCGATGCTAAAGATGCGGGCTACTTGATCAAACGTCATCTCCGTCCATc                                                              >  1:2907732/1‑93 (MQ=255)

                             aGCGCCGCCGGGCTGGCGATTTTGCAAAACCGTTTGCAGGTTGCCGATGCTAAAGATGCGGGCTACTTGATCAAACGTCATCTCCGTCCATcg                                                             >  1:935263/1‑93 (MQ=255)

                              gcgcCGCCGGGCTGGCGATTTTGCAAAACCGTTTGCAGGTTGCCGATGCTAAAGATGCGGGCTACTTGATCAAACGTCATCTCCGTCCATcgc                                                            <  2:3473393/93‑1 (MQ=255)

                              gcgcCGCCGGGCTGGCGATTTTGCAAAACCGTTTGCAGGTTGCCGATGCTAAAGATGCGGGCTACTTGATCAAACGTCATCTCCGTCCATcgc                                                            <  1:1328948/93‑1 (MQ=255)

                              gcgcCGCCGGGCTGGCGATTTTGCAAAACCGTTTGCAGGTTGCCGATGCTAAAGATGCGGACTACTTGATCAAACGTCATCTCCGACCATcgc                                                            <  2:6363469/93‑1 (MQ=255)

                                 ccgccgGGCTGGCGATTTTGCAAAACCGTTTGCAGGTTGCCGATGCTAAAGATGCGGGCTACTTGATCAAACGTCATCTCCGTCCATcgccgc                                                         <  2:3880289/93‑1 (MQ=255)

                                   gccgGGCTGGCGATTTTGCAAAACCGTTTGCAGGTTGCCGATGCTAAAGATGCGGGCTACTTGATCAAACGTCATCTCCGTCCATCGCCGCGt                                                       >  2:2834380/1‑93 (MQ=255)

                                    ccgGGCTGGCGATTTTGCAAAACCGTTTGCACGTTGCCGATGCTAAAGATGCGGGCTACTTGATCAAACGTCATCTCCGTCCATCGCCGCGTa                                                      >  2:1533261/1‑93 (MQ=255)

                                      gggCTGGCGATTTTGCAAAACCGTTTGCAGGTTGCCGATGCTAAAGATGCGGGCTACTTGATCAAACGTCATCTCCGTCCATCGCCGCGTAtt                                                    <  2:3153895/93‑1 (MQ=255)

                                      gggCTGGCGATTTTGCAAAACCGTTTGCAGGTTGCCGATGCTAAAGATGCGGGCTACTTGATCAAACGTCATCTCCGTCCATCGCCGCGTAtt                                                    >  1:6236480/1‑93 (MQ=255)

                                      gggCTGGCGATTTTGCAAAACCGTTTGCAGGTTGCCGATGCTAAAGATGCGGGCTACTTGATCAAACGTCATCTCCGACCATCGCCGCGTAtt                                                    >  1:4429175/1‑93 (MQ=255)

                                      cggCTGGCGATTTTGCAAAACCGTTTGCAGGTTGCCGATGCTAAAGATGCGGGCTACTTGATCAAACGTCATCTCCGTCCATCGCCGCGTAtt                                                    <  2:234497/92‑1 (MQ=255)

                                       ggCTGGCGATTTTGCAAAACCGTTTGCAGGTTGCCGATGCTAAAGATGCGGGCTACTTGATCAAACGTCATCTCCGTCCATCGCCGCGTAttt                                                   <  1:4715320/93‑1 (MQ=255)

                                        gCTGGCGATTTTGCAAAACCGTTTGCAGGTTGCCGATGCTAAAGATGCGGGCTACTTGATCAAACGTCATCTCCGTCCATCGCCGCGTAtttt                                                  <  2:4610221/93‑1 (MQ=255)

                                         cTGGCGATTTTGCAAAACCGTTTGCAGGTTGCCGATGCTAAAGATGCGGGCTACTTGATCAAACGTCATCTCCGTCCATCGCCGCGTATTTTa                                                 >  2:5560297/1‑93 (MQ=255)

                                           ggCGATTTTGCAAAACCGTTTGCAGGTTGCCGATGCTAAAGATGCGGGCTACTTGATCAAACGTCATCTCCGTCCATCGCCGCGTATTTTACa                                               >  1:1685017/1‑93 (MQ=255)

                                           ggCGATTTTGCAAAACCGTTTGCAGGTTGCCGATGCTAAAGATGCGGGCTACTTGATCAAACGTCATCTCCGTCCATCGCCGCGTATTTTACa                                               >  1:5531436/1‑93 (MQ=255)

                                           ggCGATTTTGCAAAACCGTTTGCAGGTTGCCGATGCTAAAGATGCGGACTACTTGATCAAACGTCATCTCCGTCCATCGCCGCGTATTTTACa                                               >  1:3628381/1‑93 (MQ=255)

                                               aTTTTGCAAAACCGTTTGCAGGTTGCCGATGCTAAAGATGCGGGCTACTTGATCAAACGTCATCTCCGTCCATCGCCGCGTATTTTACAgggg                                           <  1:4228794/93‑1 (MQ=255)

                                               aTTTTGCAAAACCGTTTGCAGGTTGCCGATGCTAAAGATGCGGGCTACTTGATCAAACGTCATCTCCGTCCATCGCCGCGTATTTTACAgggg                                           <  1:5957800/93‑1 (MQ=255)

                                               aTTTTGCAAAACCGTTTGCAGGTTGCCGATGCTAAAGATGCGGGCTACTTGATCAAACGTCATCTCCGTCCATCGCCGCGTATTTTACAgggg                                           <  1:5587279/93‑1 (MQ=255)

                                               aTTTTGCAAAACCGTTTGCAGGTTGCCGATGCTAAAGATGCGGACTACTTGATCAAACGTCATCTCCGTCCATCGCCGCGTATTTTACAgggg                                           >  2:1250917/1‑93 (MQ=255)

                                                  ttGCAAAACCGTTTGCAGGTTGCCGATGCTAAAGATGCGGGCTACTTGATCAAACGTCATCTCCGTCCATCGCCGCGTATTTTACAGGggcag                                        <  2:3274186/93‑1 (MQ=255)

                                                  ttGCAAAACCGTTTGCAGGTTGCCGATGCTAAAGATGCGGGCTACTTGATCAAACGTCATCTCCGTCCATCGCCGCGTATTTTACAGGggcag                                        <  1:2289454/93‑1 (MQ=255)

                                                   tGCAAAACCGTTTGCAGGTTGCCGATGCTAAAGATGCGGGCTACTTGATCAAACGTCATCTCCGTCCATCGCCGCGTATTTTACAGGggcagg                                       >  1:6268267/1‑93 (MQ=255)

                                                     cAAAACCGTTTGCAGGTTGCCGATGCTAAAGATGCGGGCTACTTGATCAAACGTCATCTCCGTCCATCGCCGCGTATTTTACAGGggcaggca                                     <  1:745666/93‑1 (MQ=255)

                                                     cAAAACCGTTTGCAGGTTGCCGATGCTAAAGATGCGGGCTACTTGATCAAACGTCATCTCCGTCCATCGCCGCGTATTTTACAGGggcaggca                                     <  1:2679160/93‑1 (MQ=255)

                                                     cAAAACCGTTTGCAGGTTGCCGATGCTAAAGATGCGGGCTACTTGATCAAACGTCATCTCCGTCCATCGCCGCGTATTTTACAGGggcaggca                                     <  1:5902540/93‑1 (MQ=255)

                                                     cAAAACCGTTTGCAGGTTGCCGATGCTAAAGATGCGGGCTACTTGATCAAACGTCATCTCCGTCCATCGCCGCGTATTTTACAGGggcaggca                                     >  2:455661/1‑93 (MQ=255)

                                                      aaaaCCGTTTGCAGGTTGCCGATGCTAAAGATGCGGGCTACTTGATCAAACGTCATCTCCGTCCATCGCCGCGTATTTTACAGGGGCAGGCAc                                    <  2:3324367/93‑1 (MQ=255)

                                                      aaaaCCGTTTGCAGGTTGCCGATGCTAAAGATGCGGGCTACTTGATCAAACGTCATCTCCGTCCATCGCCGCGTATTTTACAGGGGCAGGCAc                                    <  1:6305837/93‑1 (MQ=255)

                                                       aaaCCGTTTGCAGGTTGCCGATGCTAAAGATGCGGGCTACTTGATCAAACGTCATCTCCGTCCATCGCCGCGTATTTTACAGGGGCAGGCACt                                   >  1:2061251/1‑93 (MQ=255)

                                                          ccGTTTGCAGGTTGCCGATGCTAAAGATGCGGGCTACTTGATCAAACGTCATCTCCGTCCATCGCCGCGTATTTTACAGGGGCAGGCACTgcg                                >  2:4720019/1‑93 (MQ=255)

                                                          ccGTTTGCAGGTTGCCGATGCTAAAGATGCGGGCTACTTGATCAAACGTCATCTCCGTCCATCGCCGCGTATTTTACAGGGGCAGGCACTgcg                                >  2:663547/1‑93 (MQ=255)

                                                           cGTTTGCAGGTTGCCGATGCTAAAGATGCGGGCTACTTGATCAAACGTCATCTCCGTCCATCGCCGCGTATTTTACAGGGGCAGGCACTgcgc                               >  2:3853749/1‑93 (MQ=255)

                                                           cGTTTGCAGGTTGCCGATGCTAAAGATGCGGGCTACTTGATCAAACGTCATCTCCGTACATCGCCGCGTATTTTACAGGGGCAGGCACTgcgc                               <  2:517279/93‑1 (MQ=255)

                                                           cGTTTGCAGGTTGCAGATGCTAAAGATGCGGGCTACTTGATCAAACGTCATCTCCGTCCATCGCCGCGTATTTTACAGGGGCAGGCACTgcgc                               <  1:3471563/93‑1 (MQ=255)

                                                             tttGCAGGTTGCCGATGCTAAAGATGCGGGCTACTTGATCAAACGTCATCTCCGTCCATCGCCGCGTATTTTACAGGGGCAGGCACTGCGCGa                             >  2:5493320/1‑93 (MQ=255)

                                                               tGCAGGTTGCCGATGCTAAAGATGCGGGCTACTTGATCAAACGTCATCTCCGTCCATCGCCGCGTATTTTACAGGGGCAGGCACTGCGCGATc                           <  1:1524435/93‑1 (MQ=255)

                                                                  aGGTTGCCGATGCTAAAGATGCGGGCTACTTGATCAAACGTCATCTCCGTCCATCGCCGCGTATTTTACAGGGGCAGGCACTGCGCGATCTgg                        >  2:159819/1‑93 (MQ=255)

                                                                   ggTTGCCGATGCTAAAGATGCGGGCTACTTGATCAAACGTCATCTCCGTCCATCGCCGCGTATTTTACAGGGGCAGGCACTGCGCGATCTGGc                       >  1:1661065/1‑93 (MQ=255)

                                                                   ggTTGCCGATGCTAAAGATGCGGGCTACTTGATCAAACGTCATCTCCGTCCATCGCCGCGTATTTTACAGGGGCAGGCACTGCGCGATCTGGc                       <  1:4663674/93‑1 (MQ=255)

                                                                    gTTGCCGATGCTAAAGATGCGGGCTACTTGATCAAACGTCATCTCCGTCCATCGCCGCGTATTTTACAGGGGCAGGCACTGCGCGATCTGGCa                      <  2:5662751/93‑1 (MQ=255)

                                                                      tGCCGATGCTAAAGATGCGGGCTACTTGATCAAACGTCATCTCCGTCCATCGCCGCGTATTTTACAGGGGCAGGCACTGCGCGATCTGGCaaa                    >  1:2753196/1‑93 (MQ=255)

                                                                      tGCCGATGCTAAAGATGCGGGCTACTTGATCAAACGTCATCTCCGTCCATCGCCGCGTATTTTACAGGGGCAGGCACTGCGCGATCTGGCaaa                    >  2:3353744/1‑93 (MQ=255)

                                                                       gCCGATGCTAAAGATGCGGGCTACTTGATCAAACGTCATCTCCGTCCATCGCCGCGTATTTTACAGGGGCAGGCACTGCGCGATCTGGCAAAt                   <  1:2982634/93‑1 (MQ=255)

                                                                        ccGATGCTAAAGATGCGGGCTACTTGATCAAACGTCATCTCCGTCCATCGCCGCGTATTTTACAGGGGCAGGCACTGCGCGATCTGGCAAAtt                  <  2:5853420/93‑1 (MQ=255)

                                                                          gATGCTAAAGATGCGGGCTACTTGATCAAACGTCATCTCCGTCCATCGCCGCGTATTTTACAGGGGCAGGCACTGCGCGATCTGGCAAATTCa                <  1:551190/93‑1 (MQ=255)

                                                                           aTGCTAAAGATGCGGGCTACTTGATCAAACGTCATCTCCGTCCATCGCCGCGTATTTTACAGGGGCAGGCACTGCGCGATCTGGCAAATTCAg               >  2:4222787/1‑93 (MQ=255)

                                                                           aTGCTAAAGATGCGGGCTACTTGATCAAACGTCATCTCCGTCCATCGCCGCGTATTTTACAGGGGCAGGCACTGCGCGATCTGGCAAATTCAg               <  2:2431701/93‑1 (MQ=255)

                                                                            tGCTAAAGATGCGGGCTACTTGATCAAACGTCATCTCCGTCCATCGCCGCGTATTTTACAGGGGCAGGCACTGCGCGATCTGGCAAATTCAGc              >  1:1362591/1‑93 (MQ=255)

                                                                            tGCTAAAGATGCGGGCTACTTGATCAAACGTCATCTCCGTCCATCGCCGCGTATTTTACAGGGGCAGACACTGCGCGATCTGGCAAATTCAGc              >  1:5680245/1‑93 (MQ=255)

                                                                             gCTAAAGATGCGGGCTACTTGATCAAACGTCATCTCCGTCCATCGCCGCGTATTTTACAGGGGCAGGCACTGCGCGATCTGGCAAATTCAGcc             >  1:1030532/1‑93 (MQ=255)

                                                                             gCTAAAGATGCGGGCTACTTGATCAAACGTCATCTCCGTCCATCGCCGCGTATTTTACAGGGGCAGGCACTGCGCGATCTGGCAAATTCAGca             >  1:2451551/1‑92 (MQ=255)

                                                                              cTAAAGATGCGGGCTACTTGATCAAACGTCATCTCCGTCCATCGCCGCGTATTTTACAGGGGCAGGCACTGCGCGATCTGGCAAATTCAGCCa            <  1:4808347/93‑1 (MQ=255)

                                                                               tAAAGATGCGGGCTACTTGATCAAACGTCATCTCCGTCCATCGCCGCGTATTTTACAGGGGCAGGCACTGCGCGATCTGGCAAATTCAGCCAt           >  2:6062080/1‑93 (MQ=255)

                                                                                 aaGATGCGGGCTACTTGATCAAACGTCATCTCCGTCCATCGCCGCGTATTTTACAGGGGCAGGCACTGCGCGATCTGGCAAATTCAGCCATca         >  2:3074996/1‑92 (MQ=255)

                                                                                  aGATGCGGGCTACTTGATCAAACGTCATCTCCGTCCATCGCCGCGTATTTTACAGGGGCAGGCACTGCGCGATCTGGCAAATTCAGCCATCGa        >  1:4702742/1‑93 (MQ=255)

                                                                                  aGATGCGGGCTACTTGATCAAACGTCATCTCCGTCCATCGCCGCGTATTTTACAGGGGCAGGCACTGCGCGATCTGGCAAATTCAGCCATCGa        <  1:1245215/93‑1 (MQ=255)

                                                                                   gATGCGGGCTACTTGATCAAACGTCATCTCCGTCCATCGCCGCGTATTTTACAGGGGCAGGCACTGCGCGATCTGGCAAATTCAGCCATCGAt       >  1:2840375/1‑93 (MQ=255)

                                                                                   gATGCGGGCTACTTGATCAAACGTCATCTCCGTCCATCGCCGCGTATTTTACAGGGGCAGGCACTGCGCGATCTGGCAAATTCAGCCATCGAt       >  1:3740339/1‑93 (MQ=255)

                                                                                   gATGCGGGCTACTTGATCAAACGTCATCTCCGTCCATCGCCGCGTATTTTACAGGGGCAGGCACTGCGCGATCTGGCAAATTCAGCCATCGAt       >  1:5353080/1‑93 (MQ=255)

                                                                                    aTGCGGGCTACTTGATCAAACGTCATCTCCGTCCATCGCCGCGTATTTTACAGGGGCAGGCACTGCGCGATCTGGCAAATTCAGCCATCGAtc      <  2:3714107/93‑1 (MQ=255)

                                                                                    aTGCGGGCTACTTGATCAAACGTCATCTCCGTCCATCGCCGCGTATTTTACAGGGGCAGGCACTGCGCGATCTGGCAAATTCAGCCATCGAtc      <  2:1733225/93‑1 (MQ=255)

                                                                                     tGCGGACTACTTGATCAAACGTCATCTCCGTCCATCGCCGCGTATTTTACAGGGGCAGGCACTGCGCGATCTGGCAAATTCAGCCATCGAtct     >  2:525658/1‑93 (MQ=255)

                                                                                        ggACTACTTGATCAAACGTCATCTCCGTCCATCGCCGCGTATTTTACAGGGGCAGGCACTGCGCGATCTGGCAAATTCAGCCATCGAtctctc  <  1:4017472/93‑1 (MQ=255)

GGATCTATGTGACCGGTACACCGGGCGATAGCGCCGCCGGGCTGGCGATTTTGCAAAACCGTTTGCAGGTTGCCGATGCTAAAGATGCGGACTACTTGATCAAACGTCATCTCCGTCCATCGCCGCGTATTTTACAGGGGCAGGCACTGCGCGATCTGGCAAATTCAGCCATCGATCTCTC  >  NC_000913/435318‑435498

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 22 ≤` `ATCG/ATCG` `< 29 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

BRESEQ :: bam2aln output

CTGGATCTATGTGACCGGTACACCGGGCGATAGCGCCGCCGGGCTGGCGATTTTGCAAAACCGTTTGCAGGTTGCCGATGCTAAAGATGCGGACTACTTGATCAAACGTCATCTCCGTCCATCGCCGCGTATTTTACAGGGGCAGGCACTGCGCGATCTGGCAAATTCAGCCATCGATCTCTCTGA  >  NC_000913/435316‑435501

CTGGATCTATGTGACCGGTACACCGGGCGATAGCGCCGCCGGGCTGGCGATTTTGCAAAACCGTTTGCAGGTTGCCGATGCTAAAGATGCGGGC                                                                                              <  SRR4302537.3625190/94‑1 (MQ=60)

CTGGATCTATGTGACCGGTACACCGGGCGATAGCGCCGCCGGGCTGGCGATTTTGCAAAACCGTTTGCAGGTTGCCGATGCTAAAGATGCGGGC                                                                                              <  SRR4302537.1186188/94‑1 (MQ=60)

CTGGATCTATGTGACCGGTACACCGGGCGATAGCGCCGCCGGGCTGGCGATTTTGCAAAACCGTTTGCAGGTTGCCGATGCTAAAGATGCGGGC                                                                                              >  SRR4302537.1828665/1‑94 (MQ=60)

  GGATCTATGTGACCGGTACACCGGGCGATAGCGCCGCCGGGCTGGCGATTTTGCAAAACCGTTTGCAGGTTGCCGATGCTAAAGATGCGGGCTA                                                                                            <  SRR4302537.416574/94‑1 (MQ=60)

  GGATCTATGTGACCGGTACACCGGGCGATAGCGCCGCCGGGCTGGCGATTTTGCAAAACCGTTTGCAGGTTGCCGATGCTAAAGATGCGGGCTA                                                                                            <  SRR4302537.3256063/94‑1 (MQ=60)

   GATCTATGTGACCGGTACACCGGGCGATAGCGCCGCCGGGCTGGCGATTTTGCAAAACCGTTTGCAGGTTGCCGATGCTAAAGATGCGGGCTAC                                                                                           >  SRR4302537.252351/1‑94 (MQ=60)

   GATCTATGTGACCGGTACACCGGGCGATAGCGCCGCCGGGCTGGCGATTTTGCAAAACCGTTTGCAGGTTGCCGATGCTAAAGATGCGGGCTAC                                                                                           <  SRR4302537.6024646/94‑1 (MQ=60)

   GATCTATGTGACCGGTACACCGGGCGATAGCGCCGCCGGGCTGGCGATTTTGCAAAACCGTTTGCAGGTTGCCGATGCTAAAGATGCGGGCTAC                                                                                           <  SRR4302537.2792716/94‑1 (MQ=60)

   GATCTATGTGACCGGTACACCGGGCGATAGCGCCGCCGGGCTGGCGATTTTGCAAAACCGTTTGCAGGTTGCCGATGCTAAAGATGCGGGCTAC                                                                                           >  SRR4302537.1860028/1‑94 (MQ=60)

   GATCTATGTGACCGGTACACCGGGCGATAGCGCCGCCGGGCTGGCGATTTTGCAAAACCGTTTGCAGGTTGCCGATGCTAAAGATGCGGGCTAC                                                                                           >  SRR4302537.4655667/1‑94 (MQ=60)

    ATCTATGTGACCGGTACACCGGGCGATAGCGCCGCCGGGCTGGCGATTTTGCAAAACCGTTTGCAGGTTGCCGATGCTAAAGATGCGGGCTACT                                                                                          >  SRR4302537.6277915/1‑94 (MQ=60)

    ATCTATGTGACCGGTACACCGGGCGATAGCGCCGCCGGGCTGGCGATTTTGCAAAACCGTTTGCAGGTTGCCGATGCTAAAGATGCGGGCTACT                                                                                          <  SRR4302537.645413/94‑1 (MQ=60)

     TCTATGTGACCGGTACACCGGGCGATAGCGCCGCCGGGCTGGCGATTTTGCAAAACCGTTTGCAGGTTGCCGATGCTAAAGATGCGGGCTACTT                                                                                         <  SRR4302537.2375709/94‑1 (MQ=60)

      CTATGTGAGCGGTACACCGGGCGATAGCGCCGCCGGGCTGGCGATTTTGCAAAACCGTTTGCAGGTTGCCGATGCTAAAGATGCGGGCTACTTG                                                                                        <  SRR4302537.5885036/94‑1 (MQ=60)

      CTATGTGACCGGTACACCGGGCGATAGCGCCGCCGGGCTGGCGATTTTGCAAAACCGTTTGCAGGTTGCCGATGCTAAAGATGCGGGCTACTTG                                                                                        <  SRR4302537.3303114/94‑1 (MQ=60)

      CTATGTGACCGGTACACCGGGCGATAGCGCCGCCGGGCTGGCGATTTTGCAAAACCGTTTGCAGGTTGCCGATGCTAAAGATGCGGGCTACTTG                                                                                        <  SRR4302537.5136896/94‑1 (MQ=60)

      CTATGTGACCGGTACACCGGGCGATAGCGCCGCCGGGCTGGCGATTTTGCAAAACCGTTTGCAGGTTGCCGATGCTAAAGATGCGGGCTACTTG                                                                                        <  SRR4302537.1939350/94‑1 (MQ=60)

       TATGTGACCGGTACACCGGGCGATAGCGCCGCCGGGCTGGCGATTTTGCAAAACCGTTTGCAGGTTGCCGATGCTAAAGATGCGGGCTACTTGA                                                                                       >  SRR4302537.6247517/1‑94 (MQ=60)

       TATGTGACCGGTACACCGGGCGATAGCGCCGCCGGGCTGGCGATTTTGCAAAACCGTTTGCAGGTTGCCGATGCTAAAGATGCGGGCTACTTGA                                                                                       >  SRR4302537.3368555/1‑94 (MQ=60)

        ATGTGACCGGTACACCGGGCGATAGCGCCGCCGGGCTGGCGATTTTGCAAAACCGTTTGCAGGTTGCCGATGCTAAAGATGCGGGCTACTTGAT                                                                                      >  SRR4302537.820611/1‑94 (MQ=60)

         TGTGACCGGTACACCGGGCGATAGCGCCGCCGGGCTGGCGATTTTGCAAAACCGTTTGCAGGTTGCCGATGCTAAAGATGCGGGCTACTTGATC                                                                                     <  SRR4302537.6476058/94‑1 (MQ=60)

         TGTGACCGGTACACCGGGCGATAGCGCCGCCGGGCTGGCGATTTTGCAAAACCGTTTGCAGGTTGCCGATGCTAAAGATGCGGGCTACTTGATC                                                                                     <  SRR4302537.529743/94‑1 (MQ=60)

          GTGACCGGTACACCGGGCGATAGCGCCGCCGGGCTGGCGATTTTGCAAAACCGTTTGCAGGTTGCCGATGCTAAAGATGCGGGCTACTTGATCA                                                                                    <  SRR4302537.5161143/94‑1 (MQ=60)

          GTGACCGGTACACCGGGCGATAGCGCCGCCGGGCTGGCGATTTTGCAAAACCGTTTGCAGGTTGCCGATGCTAAAGATGCGGGCTACTTGATCA                                                                                    <  SRR4302537.1120356/94‑1 (MQ=60)

            GACCGGTACACCGGGCGATAGCGCCGCCGGGCTGGCGATTTTGCAAAACCGTTTGCAGGTTGCCGATGCTAAAGATGCGGGCTACTTGATCAAA                                                                                  <  SRR4302537.5803581/94‑1 (MQ=60)

             ACCGGTACACCGGGCGATAGCGCCGCCGGGCTGGCGATTTTGCAAAACCGTTTGCAGGTTGCCGATGCTAAAGATGCGGGCTACTTGATCAAAC                                                                                 >  SRR4302537.6119088/1‑94 (MQ=60)

             ACCGGTACACCGGGCGATAGCGCCGCCGGGCTGGCGATTTTGCAAAACCGTTTGCAGGTTGCCGAAGCTAAAGATGCGGGCTACTTGATCAAAC                                                                                 >  SRR4302537.6259735/1‑94 (MQ=60)

              CCGGTACACCGGGCGATAGCGCCGCCGGGCTGGCGATTTTGCAAAACCGTTTGCAGGTTGCCGTTGCTAAAGATGCGGGCTCCTTGAGCAAACG                                                                                >  SRR4302537.5014839/1‑94 (MQ=60)

              CCGGTACACCGGGCGATAGCGCCGCCGGGCTGGCGATTTTGCAAAACCGTTTGCAGGTTGCCGATGCTAAAGATGCGGGCTACTTGATCAAACG                                                                                <  SRR4302537.2806805/94‑1 (MQ=60)

                 GTACACCGGGCGATAGCGCCGCCGGGCTGGCGATTTTGCAAAACCGTTTGCAGGTTGCCGATGCTAAAGATGCGGGCTACTTGATCAAACGTCA                                                                             <  SRR4302537.5870986/94‑1 (MQ=60)

                 GTACACCGGGCGATAGCGCCGCCGGGCTGGCGATTTTGCAAAACCGTTTGCAGGTTGCCGATGCTAAAGATGCGGGCTACTTGATCAAACGTCA                                                                             <  SRR4302537.4741410/94‑1 (MQ=60)

                   ACACCGGGCGATAGCGCCGCCGGGCTGGCGATTTTGCAAAACCGTTTGCAGGTTGCCGATGCTAAAGATGCGGGCTACTTGATCAAACGTCATC                                                                           <  SRR4302537.2938966/94‑1 (MQ=60)

                    CACCGGGCGATAGCGCCGCCGGGCTGGCGATTTTGCAAAACCGTTTGCAGGTTGCCGATGCTAAAGATGCGGGCTACTTGATCAAACGTCATCT                                                                          <  SRR4302537.5218879/94‑1 (MQ=60)

                     ACCGGGCGATAGCGCCGCCGGGCTGGCGATTTTGCAAAACCGTTTGCAGGTTGCCGATGCTAAAGATGCGGGCTACTTGATCAAACGTCATCTC                                                                         <  SRR4302537.5026114/94‑1 (MQ=60)

                      CCGGGCGATAGCGCCGCCGGGCTGGCGATTTTGCAAAACCGTTTGCAGGTTGCCGATGCTAAAGATGCGGGCTACTTGATCAAACGTCATCTCC                                                                        <  SRR4302537.6424340/94‑1 (MQ=60)

                      CCGGGCGATAGCGCCGCCGGGCTGGCGATTTTGCAAAACCGTTTGCAGGTTGCCGATGCTAAAGATGCGGGCTACTTGATCAAACGTCATCTCC                                                                        >  SRR4302537.3256609/1‑94 (MQ=60)

                      CCGGGCGATAGCGCCGCCGGGCTGGCGATTTTGCAAAACCGTTTGCAGGTTGCCGATGCTAAAGATGCGGGCTACTTGATCAAACGTCATCTCC                                                                        <  SRR4302537.4921979/94‑1 (MQ=60)

                       CGGGCGATAGCGCCGCCGGGCTGGCGATTTTGCAAAACCGTTTGCAGGTTGCCGATGCTAAAGATGCGGGCTACTTGATCAAACGTCATCTCCG                                                                       >  SRR4302537.6430041/1‑94 (MQ=60)

                       CGGGCGATAGCGCCGCCGGGCTGGCGATTTTGCAAAACCGTTTGCAGGTTGCCGATGCTAAAGATGCGGGCTACTTGATCAAACGTCATCTCCG                                                                       <  SRR4302537.1585763/94‑1 (MQ=60)

                       CGGGCGATAGCGCCGCCGGGCTGGCGATTTTGCAAAACCGTTTGCAGGTTGCCGATGCTAAAGATGCGGACTACTTGATCAAACGTCATCTCCG                                                                       >  SRR4302537.566907/1‑94 (MQ=60)

                        GGGCGATAGCGCCGCCGGGCTGGCGATTTTGCAAAACCGTTTGCAGGTTGCCGATGCTAAAGATGCGGGCTACTTGATCAAACGTCATCTCCGT                                                                      >  SRR4302537.354185/1‑94 (MQ=60)

                         GGCGATAGCGCCGCCGGGCTGGCGATTTTGCAAAACCGTTTGCAGGTTGCCGATGCTAAAGATGCGGGCTACTTGATCAAACGTCATCTCCGTC                                                                     >  SRR4302537.5358982/1‑94 (MQ=60)

                         GGCGATAGCGCCGCCGGGCTGGCGATTTTGCAAAACCGTTTGCAGGTTGCCGATGCTAAAGATGCGGGCTACTTGATCAAACGTCATCTCCGTC                                                                     <  SRR4302537.4535081/94‑1 (MQ=60)

                          GCGATAGCGCCGCCGGGCTGGCGATTTTGCAAAACCGTTTGCAGGTTGCCGATGCTAAAGATGCGGGCTACTTGATCAAACGTCATCTCCGTCC                                                                    <  SRR4302537.255942/94‑1 (MQ=60)

                          GCGATAGCGACGCCGGGCTGGCGATTTCGCAAAACCGTTTGCAGGTTGCCGATGCTAAAGATGCGGGCTACTTGATCAAACGTCATCTCCGTCC                                                                    <  SRR4302537.2699863/94‑1 (MQ=60)

                           CGATAGCGCCGCCGGGCTGGCGATTTTGCAAAACCGTTTGCAGGTTGCCGATGCTAAAGATGCGGGCTACTTGATCAAACGTCATCTCCGTCCA                                                                   <  SRR4302537.3027927/94‑1 (MQ=60)

                           CGATAGCGCCGCCGGGCTGGCGATTTTGCAAAACCGTTTGCAGGTTGCCGATGCTAAAGATGCGGGCTACTTGATCAAACGTCATCTCCGTCCA                                                                   <  SRR4302537.4956283/94‑1 (MQ=60)

                           CGATAGCGCCGCCGGGCTGGCGATTTTGCAAAACCGTTTGCAGGTTGCCGATGCTAAAGATGCGGGCTACTTGATCAAACGTCATCTCCGTCCA                                                                   >  SRR4302537.4102121/1‑94 (MQ=60)

                            GATAGCGCCGCCGGGCTGGCGATTTTGCAAAACCGTTTGCAGGTTGCCGATGCTAAAGATGCGGGCTACTTGATCAAACGTCATCTCCGTCCAT                                                                  >  SRR4302537.4649311/1‑94 (MQ=60)

                            GATAGCGCCGCCGGGCTGGCGATTTTGCAAAACCGTTTGCAGGTTGCCGATGCTAAAGATGCGGGCTACTTGATCAAACGTCATCTCCGTCCAT                                                                  >  SRR4302537.2275428/1‑94 (MQ=60)

                             ATAGCGCCGCCGGGCTGGCGATTTTGCAAAACCGTTTGCAGGTTGCCGATGCTAAAGATGCGGGCTACTTGATCAAACGTCATCTCCGTCCATC                                                                 >  SRR4302537.2928158/1‑94 (MQ=60)

                             ATAGCGCCGCCGGGCTGGCGATTTTGCAAAACCGTTTGCAGGTTGCCGATGCTAAAGATGCGGGCTACTTGATCAAACGTCATCTCCGTCCATC                                                                 >  SRR4302537.845302/1‑94 (MQ=60)

                              TAGCGCCGCCGGGCTGGCGATTTTGCAAAACCGTTTGCAGGTTGCCGATGCTAAAGATGCGGGCTACTTGATCAAACGTCATCTCCGTCCATCG                                                                >  SRR4302537.941709/1‑94 (MQ=60)

                              TAGCGCCGCCGGGCTGGCGATTTTGCAAAACCGTTTGCAGGTTGCCGATGCTAAAGATGCGGGCTACTTGATCAAACGTCATCTCCGTCCATCG                                                                >  SRR4302537.5911853/1‑94 (MQ=60)

                                GCGCCTCCGGGCTGGCGATTTTGCAAAACCGTTTGCAGGTTGCCGATGCTAAAGATGCGGGCTACTTGATCAAACGTCATCTCCGTCCATCGCC                                                              <  SRR4302537.1953686/94‑1 (MQ=60)

                                GCGCCGCCGGGCTGGCGATTTTGCAAAACCGTTTGCAGGTTGCCGATGCTAAAGATGCGGGCTACTTGATCAAACGTCATCTCCGTCCATCGCC                                                              <  SRR4302537.1338213/94‑1 (MQ=60)

                                 CGCCGCCGGGCTGGCGATTTTGCAAAACCGTTTGCAGGTTGCCGATGCTAAAGATGCGGGCTACTTGATCAAACGTCATCTCCGTCCATCGCCG                                                             >  SRR4302537.5525053/1‑94 (MQ=60)

                                   CCGCCGGGCTGGCGATTTTGCAAAACCGTTTGCAGGTTGCCGATGCTAAAGATGCGGGCTACTTGATCAAACGTCATCTCCGTCCATCGCCGCG                                                           <  SRR4302537.6344804/94‑1 (MQ=60)

                                      CCGGGCTGGCGATTTTGCAAAACCGTTTGCAGGTTGCCGATGCTAAAGATGCGGGCTACTTGATCAAACGTCATCTCCGTCCATCGCCGCGTAT                                                        >  SRR4302537.1496371/1‑94 (MQ=60)

                                       CGGGCTGGCGATTTTGCAAAACCGTTTGCAGGTTGCCGATGCTAAAGATGCGGGCTACTTGATCAAACGTCATCTCCGTCCATCGCCGCGTATT                                                       >  SRR4302537.6279367/1‑94 (MQ=60)

                                       CGGGCTGGCGATTTTGCAAAACCGTTTGCAGGTTGCCGATGCTAAAGATGCGGGCTACTTGATCAAACGTCATCTCCGACCATCGCCGCGTATT                                                       >  SRR4302537.4459762/1‑94 (MQ=60)

                                        GGGCTGGCGATTTTGCAAAACCGTTTGCAGGTTGCCGATGCTAAAGATGCGGGCTACTTGATCAAACGTCATCTCCGTCCATCGCCGCGTATTT                                                      <  SRR4302537.4897590/94‑1 (MQ=60)

                                         GGCTGGCGATTTTGCAAAACCGTTTGCAGGTTGCCGATGCTAAAGATGCGGGCTACTTGATCAAACGTCATCTCCGTCCATCGCCGCGTATTTT                                                     <  SRR4302537.4747932/94‑1 (MQ=60)

                                         GGCTGGCGATTTTGCAAAACCGTTTGCAGGTTGCCGATGCTAAAGATGCGGGCTACTTGATCAAACGTCATCTCCGTCCATCGCCGCGTATTTT                                                     <  SRR4302537.4507657/94‑1 (MQ=60)

                                          GCTGGCGATTTTGCAAAACCGTTTGCAGGTTGCCGATGCTAAAGATGCGGGCTACTTGATCAAACGTCATCTCCGTCCATCGCCGCGTATTTTA                                                    >  SRR4302537.1930989/1‑94 (MQ=60)

                                            TGGCGATTTTGCAAAACCGTTTGCAGGTTGCCGATGCTAAAGATGCGGGCTACTTGATCAAACGTCATCTCCGTCCATCGCCGCGTATTTTACA                                                  >  SRR4302537.1696812/1‑94 (MQ=60)

                                            TGGCGATTTTGCAAAACCGTTTGCAGGTTGCCGATGCTAAAGATGCGGGCTACTTGATCAAACGTCATCTCCGTCCATCGCCGCGTATTTTACA                                                  >  SRR4302537.5569197/1‑94 (MQ=60)

                                            TGGCGATTTTGCAAAACCGTTTGCAGGTTGCCGATGCTAAAGATGCGGACTACTTGATCAAACGTCATCTCCGTCCATCGCCGCGTATTTTACA                                                  >  SRR4302537.3653955/1‑94 (MQ=60)

                                             GGCGATTTTGCAAAACCGTTTGCAGGTTGCCGATGCTAAAGATGCGGACTACTTGATCAAACGTCATCTCCGTCCATCGCCGCGTATTTTACAG                                                 >  SRR4302537.5560708/1‑94 (MQ=60)

                                               GGATTTTGCAAAACCGTTTGCAGGTTGCCGATGCTAAAGATGCGGGCTACTTGATCAAACGTCATCTCCGTCCATCGCCGCGTATTTTACAGGG                                               <  SRR4302537.1680637/94‑1 (MQ=60)

                                               CGATTTTGCAAAACCGTTTGCAGGTTGCCGATGCTAAAGATGCGGGCTACTTGATCAAACGTCATCTCCGTCCATCGCCGCGTATTTTACAGGG                                               >  SRR4302537.4749596/1‑94 (MQ=60)

                                               CGATTTTGCAAAACCGTTTGCAGGTTGCCGATGCTAAAGATGCGGGCTACTTGATCAAACGTCATCTCCGTCCATCGCCGCGTATTTTACAGGG                                               <  SRR4302537.1466415/94‑1 (MQ=60)

                                                GATTTTGCAAAACCGTTTGCAGGTTGCCGATGCTAAAGATGCGGGCTACTTGATCAAACGTCATCTCCGTCCATCGCCGCGTATTTTACAGGGG                                              >  SRR4302537.288319/1‑94 (MQ=60)

                                                GATTTTGCAAAACCGTTTGCAGGTTGCCGATGCTAAAGATGCGGGCTACTTGATCAAACGTCATCTCCGTCCATCGCCGCGTATTTTACAGGGG                                              >  SRR4302537.2302046/1‑94 (MQ=60)

                                                GATTTTGCAAAACCGTTTGCAGGTTGCCGATGCTAAAGATGCGGGCTACTTGATCAAACGTCATCTCCGTCCATCGCCGCGTATTTTACAGGGG                                              >  SRR4302537.4982570/1‑94 (MQ=60)

                                                 ATTTTGCAAAACCGTTTGCAGGTTGCCGATGCTAAAGATGCGGGCTACTTGATCAAACGTCATCTCCGTCCATCGCCGCGTATTTTACAGGGGC                                             <  SRR4302537.4258236/94‑1 (MQ=60)

                                                 ATTTTGCAAAACCGTTTGCAGGTTGCCGATGCTAAAGATGCGGGCTACTTGATCAAACGTCATCTCCGTCCATCGCCGCGTATTTTACAGGGGC                                             <  SRR4302537.5625484/94‑1 (MQ=60)

                                                 ATTTTGCAAAACCGTTTGCAGGTTGCCGATGCTAAAGATGCGGGCTACTTGATCAAACGTCATCTCCGTCCATCGCCGCGTATTTTACAGGGGC                                             <  SRR4302537.5998693/94‑1 (MQ=60)

                                                 ATTTTGCAAAACCGTTTGCAGGTTGCCGATGCTAAAGAAGCGGGCTACTTGATCAAACGTCATCTCCGTCCATCGCCGCGTATTTTACAGGGGC                                             >  SRR4302537.622239/1‑94 (MQ=60)

                                                   TTTGCAAAACCGTTTGCAGGTTGCCGATGCTAAAGATGCGGGCTACTTGATCAAACGTCATCTCCGTCCATCGCCGCGTATTTTACAGGGGCAG                                           <  SRR4302537.710817/94‑1 (MQ=60)

                                                   TTTGCAAAACCGTTTGCAGGTTGCCGATGCTAAAGATGCGGGCTACTTGATCAAACGTCATCTCCGTCCATCGCCGCATATTTTACAGGGGCAG                                           <  SRR4302537.4938169/94‑1 (MQ=60)

                                                    TTGCAAAACCGTTTGCAGGTTGCCGATGCTAAAGATGCGGGCTACTTGATCAAACGTCATCTCCGTCCATCGCCGCGTATTTTACAGGGGCAGG                                          >  SRR4302537.6311289/1‑94 (MQ=60)

                                                    TTGCAAAACCGTTTGCAGGTTGCCGATGCTAAAGATGCGGGCTACTTGATCAAACGTCATCTCCGTCCATCGCCGCGTATTTTACAGGGGCAGG                                          <  SRR4302537.2305620/94‑1 (MQ=60)

                                                    TTGCAAAACCGTTTGCAGGTTGCCGATGCTAAAGATGCGGGCTACTTGATCAAACGTCATCTCCGTCCATCGCCGCGTATTTTACAGGGGCAGG                                          <  SRR4302537.6417784/94‑1 (MQ=60)

                                                       CAAAACCGTTTGCAGGTTGCCGATGCTAAAGATGCGGGCTACTTGATCAAACGTCATCTCCGTCCATCGCCGCGTATTTTACAGGGGCAGGCAC                                       <  SRR4302537.5943041/94‑1 (MQ=60)

                                                       CAAAACCGTTTGCAGGTTGCCGATGCTAAAGATGCGGGCTACTTGATCAAACGTCATCTCCGTCCATCGCCGCGTATTTTACAGGGGCAGGCAC                                       >  SRR4302537.2341651/1‑94 (MQ=60)

                                                       CAAAACCGTTTGCAGGTTGCCGATGCTAAAGATGCGGGCTACTTGATCAAACGTCATCTCCGTCCATCGCCGCGTATTTTACAGGGGCAGGCAC                                       <  SRR4302537.3371895/94‑1 (MQ=60)

                                                       CAAAACCGTTTGCAGGTTGCCGATGCTAAAGATGCGGGCTACTTGATCAAACGTCATCTCCGTCCATCGCCGCGTATTTTACAGGGGCAGGCAC                                       <  SRR4302537.750752/94‑1 (MQ=60)

                                                       CAAAACCGTTTGCAGGTTGCCGATGCTAAAGATGCGGGCTACTTGATCAAACGTCATCTCCGTCCATCGCCGCGTATTTTACAGGGGCAGGCAC                                       <  SRR4302537.2697866/94‑1 (MQ=60)

                                                        AAAACCGTTTGCAGGTTGCCGATGCTAAAGATGCGGGCTACTTGATCAAACGTCATCTCCGTCCATCGCCGCGTATTTTACAGGGGCAGGCACT                                      <  SRR4302537.6349015/94‑1 (MQ=60)

                                                        AAAACCGTTTGCAGGTTGCCGATGCTAAAGATGCGGGCTACTTGATCAAACGTCATCTCCGTCCATCGCCGCGTATTTTACAGGGGCAGGCACT                                      >  SRR4302537.2075726/1‑94 (MQ=60)

                                                         AAACCGTTTGCAGGTTGCCGATGCTAAAGATGCGGACTACTTGATCAAACGTCATCTCCGTCCATCGCCGCGTATTTTACAGGGGCAGGCACTG                                     <  SRR4302537.5605359/94‑1 (MQ=60)

                                                             CGTTTGCAGGTTGCAGATGCTAAAGATGCGGGCTACTTGATCAAACGTCATCTCCGTCCATCGCCGCGTATTTTACAGGGGCAGGCACTGCGCG                                 <  SRR4302537.3496125/94‑1 (MQ=60)

                                                              GTTTGCAGGTTGCCGATGCTAAAGATGCGGGCTACTTGATCAAACGTCATCTCCGTCCATCGCCGCGTATTTTACAGGGGCAGGCACTGCGCGA                                >  SRR4302537.1367014/1‑94 (MQ=60)

                                                              GTTTGCAGGTTGCCGATGCTAAAGATGCGGGCTACTTGATCAAACGTCATCTCCGTCCATCGCCGCGTATTTTACAGGGGCAGGCACTGCGCGA                                >  SRR4302537.5944525/1‑94 (MQ=60)

                                                                 TGCAGGTTGCCGATGCTAAAGATGCGGGCTACTTGATCAAACGTCATCTCCGTCCATCGCCGCGTATTTTACAGGGGCAGGCACTGCGCGATCT                             <  SRR4302537.1535083/94‑1 (MQ=60)

                                                                 TGCAGGTTGCCGATGCTAAAGATGCGGGCTACTTGATCAAACGTCATCTCCGTCCATCGCCGCGTATTTTACAGGGGCAGGCACTGCGCGATCT                             <  SRR4302537.3981669/94‑1 (MQ=60)

                                                                    AGGTTGCCGATGCTAAAGATGCGGGCTACTTGATCAAACGTCATCTCCGTCCATCGCCGCGTATTTTACAGGGGCAGGCACTGCGCGATCTGGC                          >  SRR4302537.1275697/1‑94 (MQ=60)

                                                                    AGGTTGCCGATGCTAAAGATGCGGGCTACTTGATCAAACGTCATCTCCGTCCATCGCCGCGTATTTTACAGGGGCAGGCACTGCGCGATCTGGC                          >  SRR4302537.1672706/1‑94 (MQ=60)

                                                                    AGGTTGCCGATGCTAAAGATGCGGGCTACTTGATCAAACGTCATCTCCGTCCATCGCCGCGTATTTTACAGGGGCAGGCACTGCGCGATCTGGC                          <  SRR4302537.4700220/94‑1 (MQ=60)

                                                                     GGTTGCCGATGCTAAAGATGCGGGCTACTTGATCAAACGTCATCTCCGTCCATCGCCGCGTATTTTACAGGGGCAGGCACTGCGCGATCTGGCA                         <  SRR4302537.4695906/94‑1 (MQ=60)

                                                                       TTGCCGATGCTAAAGATGCGGGCTACTTGATCAAACGTCATCTCCGTCCATCGCCGCGTATTTTACAGGGGCAGGCACTGCGCGATCTGGCAAA                       >  SRR4302537.2772411/1‑94 (MQ=60)

                                                                        TGCCGATGCTAAAGATGCGGGCTACTTGATCAAACGTCATCTCCGTCCATCGCCGCGTATTTTACAGGGGCAGGCACTGCGCGATCTGGCAAAT                      <  SRR4302537.781749/94‑1 (MQ=60)

                                                                         GCCGATGCTAAAGATGCGGGCTACTTGATCAAACGTCATCTCCGTCCATCGCCGCGTATTTTACAGGGGCAGGCACTGCGCGATCTGGCAAATT                     <  SRR4302537.3003486/94‑1 (MQ=60)

                                                                          CCGATGCTAAAGATGCGGGCTACTTGATCAAACGTCATCTCCGTCCATCGCCGCGTATTTTACAGGGGCAGGCACTGCGCGATCTGGCAAATTC                    <  SRR4302537.4313210/94‑1 (MQ=60)

                                                                          CCGATGCTAAAGATGCGGGCTACTTGATCAAACGTCATCTCCGTCCATCGCCGCGTATTTTACAGGGGCAGGCACTGCGCGATCTGGCAAATTC                    >  SRR4302537.5553554/1‑94 (MQ=60)

                                                                           CGATGCTAAAGATGCGGGCTACTTGATCAAACGTCATCTCCGTCCATCGCCGCGTATTTTACAGGGGCAGGCACTGCGCGATCTGGCAAATTCA                   >  SRR4302537.4775877/1‑94 (MQ=60)

                                                                           CGATGCTAAAGATGCGGGCTACTTGATCAAACGTCATCTCCGTCCATCGCCGCGTATTTTACAGGGGCAGGCACTGCGCGATCTGGCAAATTCA                   <  SRR4302537.3968250/94‑1 (MQ=60)

                                                                            GATGCTAAAGATGCGGGCTACTTGATCAAACGTCATCTCCGTCCATCGCCGCGTATTTTACAGGGGCAGGCACTGCGCGATCTGGCAAATTCAG                  <  SRR4302537.554981/94‑1 (MQ=60)

                                                                             ATGCTAAAGATGCGGGCTACTTGATCAAACGTCATCTCCGTCCATCGCCGCGTATTTTACAGGGGCAGGCACTGCGCGATCTGGCAAATTCAGC                 >  SRR4302537.1372084/1‑94 (MQ=60)

                                                                             ATGCTAAAGATGCGGGCTACTTGATCAAACGTCATCTCCGTCCATCGCCGCGTATTTTACAGGGGCAGACACTGCGCGATCTGGCAAATTCAGC                 >  SRR4302537.5719102/1‑94 (MQ=60)

                                                                              TGCTAAAGATGCGGGCTACTTGATCAAACGTCATCTCCGTCCATCGCCGCGTATTTTACAGGGGCAGGCACTGCGCGATCTGGCAAATTCAGCC                >  SRR4302537.1037630/1‑94 (MQ=60)

                                                                              TGCTAAAGATGCGGGCTACTTGATCAAACGTCATCTCCGTCCATCGCCGCGTATTTTACAGGGGCAGGCACTGCGCGATCTGGCAAATTCAGCA                >  SRR4302537.2468800/1‑94 (MQ=60)

                                                                               GCTAAAGATGCGGGCTACTTGATCAAACGTCATCTCCGTCCATCGCCGCGTATTTTACAGGGGCAGGCACTGCGCGATCTGGCAAATTCAGCCA               <  SRR4302537.2635431/94‑1 (MQ=60)

                                                                                CTAAAGATGCGGGCTACTTGATCAAACGTCATCTCCGTCCATCGCCGCGTATTTTACAGGGGCAGGCACTGCGCGATCTGGCAAATTCAGCCAT              <  SRR4302537.4841535/94‑1 (MQ=60)

                                                                                  AAAGATGCGGGCTACTTGATCAAACGTCATCTCCGTCCATCGCCGCGTATTTTACAGGGGCAGGCACTGCGCGATCTGGCAAATTCAGCCATCG            <  SRR4302537.2261590/94‑1 (MQ=60)

                                                                                   AAGATGCGGGCTACTTGATCAAACGTCATCTCCGTCCATCGCCGCGTATTTTACAGGGGCAGGCACTGCGCGATCTGGCAAATTCAGCCATCGA           >  SRR4302537.4735280/1‑94 (MQ=60)

                                                                                    AGATGCGGGCTACTTGATCAAACGTCATCTCCGTCCATCGCCGCGTATTTTACAGGGGCAGGCACTGCGCGATCTGGCAAATTCAGCCATCGAT          <  SRR4302537.1253891/94‑1 (MQ=60)

                                                                                    AGATGCGGGCTACTTGATCAAACGTCATCTCCGTCCATCGCCGCGTATTTTACAGGGGCAGGCACTGCGCGATCTGGCAAATTCAGCCATCGAT          >  SRR4302537.5389743/1‑94 (MQ=60)

                                                                                    AGATGCGGGCTACTTGATCAAACGTCATCTCCGTCCATCGCCGCGTATTTTACAGGGGCAGGCACTGCGCGATCTGGCAAATTCAGCCATCGAT          >  SRR4302537.2860253/1‑94 (MQ=60)

                                                                                    AGATGCGGGCTACTTGATCAAACGTCATCTCCGTCCATCGCCGCGTATTTTACAGGGGCAGGCACTGCGCGATCTGGCAAATTCAGCCATCGAT          >  SRR4302537.3766609/1‑94 (MQ=60)

                                                                                     GATGCGGGCTACTTGATCAAACGTCATCTCCGTCCATCGCCGCGTATTTTACAGGGGCAGGCACTGCGCGATCTGGCAAATTCAGCCATCGATC         >  SRR4302537.4221182/1‑94 (MQ=60)

                                                                                      ATGCGGGCTACTTGATCAAACGTCATCTCCGTCCATCGCCGCGTATTTTACAGGGGCAGGCACTGCGCGATCTGGCAAATTCAGCCATCGATCT        <  SRR4302537.1668924/94‑1 (MQ=60)

                                                                                        GCGGGCTACTTGATCAAACGTCATCTCCGTCCATCGCCGCGTATTTTACAGGGGCAGGCACTGCGCGATCTGGCAAATTCAGCCATCGATCTCT      <  SRR4302537.1562093/94‑1 (MQ=60)

                                                                                        GCGGGCTACTTGATCAAACGTCATCTCCGTCCATCGCCGCGTATTTTACAGGGGCAGGCACTGCGCGATCTGGCAAATTCAGCCATCGATCTCT      <  SRR4302537.3746471/94‑1 (MQ=60)

                                                                                         CGGGCTACTTGATCAAACGTCATCTCCGTCCATCGCCGCGTATTTTACAGGGGCAGGCACTGCGCGATCTGGCAAATTCAGCCATCGATCTCTC     >  SRR4302537.5113001/1‑94 (MQ=60)

                                                                                          GGGCTACTTGATCAAACGTCATCTCCGTCCATCGCCGCGTATTTTACAGGGGCAGGCACTGCGCGATCTGGCAAATTCAGCCATCGATCTCTCT    >  SRR4302537.3011089/1‑94 (MQ=60)

                                                                                          GGACTACTTGATCAAACGTCATCTCCGTCCATCGCCGCGTATTTTACAGGGGCAGGCACTGCGCGATCTGGCAAATTCAGCCATCGATCTCTCT    <  SRR4302537.4045599/94‑1 (MQ=60)

                                                                                            GCTACTTGATCAAACGTCATCTCCGTCCATCGCCGCGTATTTTACAGGGGCAGGCACTGCGCGATCTGGCAAATTCAGCCATCGATCTCTCTGA  >  SRR4302537.116275/1‑94 (MQ=60)

CTGGATCTATGTGACCGGTACACCGGGCGATAGCGCCGCCGGGCTGGCGATTTTGCAAAACCGTTTGCAGGTTGCCGATGCTAAAGATGCGGACTACTTGATCAAACGTCATCTCCGTCCATCGCCGCGTATTTTACAGGGGCAGGCACTGCGCGATCTGGCAAATTCAGCCATCGATCTCTCTGA  >  NC_000913/435316‑435501

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 0 ≤` `ATCG/ATCG` `< 18 ≤` `ATCG/ATCG` `< 29 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`