Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I2 R1 882 254.4 12957588 98.0% 12698436 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 551,609 T→C N70S (AAC→AGC)  purK ← phosphoribosylaminoimidazole carboxylase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913551,6090TC98.0% 861.8 / 3.0 256N70S (AAC→AGC) purKphosphoribosylaminoimidazole carboxylase
Reads supporting (aligned to +/- strand):  ref base T (2/3);  new base C (127/124);  total (129/127)
Fisher's exact test for biased strand distribution p-value = 6.83e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.61e-01

Only 100 of 257 total aligned reads displayed.

AGTAACTGCCACGGTGCAGTCGGCAGGTGGAGCTTATCGAAAAGCTGCTTCTGAGTCAGACGGTCAGCAATAATCGGGAACACATCGCGGTTCACAAAGGCCGGATGGCGCGCCAGCTCGCGGGTTAATGCGGTTTCCGGCCAGCGTTCTATCTCAGCGGTAATCACGCTTTGTTGAAAA  >  NC_000913/551519‑551698
                                                                                          |                                                                                         
aGTAACTGCCACGGTGCAGTCGGCAGGTGGAGCTTATCGAAAAGCTGCTTCTGAGTCAGACGGTCAGCAATAATCGGGAACACATCGCGGCTc                                                                                         <  1:5501259/93‑1 (MQ=255)
aGTAACTGCCACGGTGCAGTCGGCAGGTGGAGCTTATCGAAAAGCTGCTTCTGAGTCAGACGGTCAGCAATAATCGGGAACACATCGCGGCTc                                                                                         <  2:5412932/93‑1 (MQ=255)
aGTAACTGCCACGGTGCAGTCGGCAGGTGGAGCTTATCGAAAAGCTGCTTCTGAGTCAGACGGTCAGCAATAATCGGGAACACATCGCGGCTc                                                                                         <  1:812661/93‑1 (MQ=255)
aGTAACTGCCACGGTGCAGTCGGCAGGTGGAGCTTATCGAAAAGCTGCTTCTGAGTCAGACGGTCAGCAATAATCGGGAACACATCGCGGCTc                                                                                         <  2:1611266/93‑1 (MQ=255)
 gTAACTGCCACGGTGCAGTCGGCAGGTGGAGCTTATCGAAAAGCTGCTTCTGAGTCAGACGGTCAGCAATCATCGGGAACACATCGCGGCTca                                                                                        >  2:5184180/1‑93 (MQ=255)
 gTAACTGCCACGGTGCAGTCGGCAGGTGGAGCTTATCGAAAAGCTGCTTCTGAGTAAGACGGTCAGCAATAATCGGGAACACATCGCGGCTcc                                                                                        >  2:3284256/1‑92 (MQ=255)
     cTGCCACGGTGCAGTCGGCAGGTGGAGCTTATCGAAAAGCTGCTTCTGAGTCAGACGGTCAGCAATAATCGGGAACACATCGCGGCTCACaaa                                                                                    <  2:4891658/93‑1 (MQ=255)
     cTGCCACGGTGCAGTCGGCAGGTGGAGCTTATCGAAAAGCTGCTTCTGAGTCAGACGGTCAGCAATAATCGGGAACACATCGCGGCTCACaaa                                                                                    <  2:1755908/93‑1 (MQ=255)
     cTGCCACGGTGCAGTCGGCAGGTGGAGCTTATCGAAAAGCTGCTTCTGAGTCAGACGGTCAGCAATAATCGGGAACACATCGCGGCTCACaaa                                                                                    <  1:2426344/93‑1 (MQ=255)
      tGCCACGGTGCAGTCGGCAGGTGGAGCTTATCGAAAAGCTGCTTCTGAGTCAGACGGTCAGCAATAATCGGGAACACATCGCGGCTCACAAAg                                                                                   <  2:321902/93‑1 (MQ=255)
       gCCACGGTGCAGTCGGCAGGTGGAGCTTATCGAAAAGCTGCTTCTGAGTCAGACGGTCAGCAATAATCGGGAACACATCGCGGCTCACAAAgg                                                                                  >  1:357516/1‑93 (MQ=255)
        ccACGGTGCAGTCGGCAGGTGGAGCTTATCGAAAAGCTGCTTCTGAGTCAGACGGTCAGCAATAATCGGGAACACATCGCGGCTCACAAAGGc                                                                                 >  2:6145250/1‑93 (MQ=255)
           cGGTGCAGTCGGCAGGTGGAGCTTATCGAAAAGCTGCTTCTGAGTCAGACGGTCAGCAATAATCGGGAACACATCGCGGCTCACAAAGGCCgg                                                                              >  1:3815797/1‑93 (MQ=255)
            ggTGCAGTCGGCAGGTGGAGCTTATCGAAAAGCTGCTTCTGAGTCAGACGGTCAGCAATAATCGGGAACACATCGCGGCTCACAAAGGCCGGa                                                                             <  2:290168/93‑1 (MQ=255)
            ggTGCAGTCGGCAGGTGGAGCTTATCGAAAAGCTGCTTCTGAGTCAGACGGTCAGCAATAATCGGGAACACATCGCGGCTCACAAAGGCCGGa                                                                             <  2:3517441/93‑1 (MQ=255)
                cAGTCGGCAGGTGGAGCTTATCGAAAAGCTGCTTCTGAGTCAGACGGTCAGCAATAATCGGGAACACATCGCGGCTCACAAAGGCCGGATGgc                                                                         <  2:2264944/93‑1 (MQ=255)
                  gTCGGCAGGTGGAGCTTATCGAAAAGCTGCTTCTGAGTCAGACGGTCAGCAATAATCGGGAACACATCGCGGCTCACAAAGGCCGGATGgcgc                                                                       >  1:4282867/1‑93 (MQ=255)
                  gTCGGCAGGTGGAGCTTATCGAAAAGCTGCTTCTGAGTCAGACGGTCAGCAATAATCGGGAACACATCGCGGCTCACAAAGGCCGGATGgcgc                                                                       <  1:677073/93‑1 (MQ=255)
                  gTCGGCAGGTGGAGCTTATCGAAAAGCTGCTTCTGAGTCAGACGGTCAGCAATAATCGGGAACACATCGCGGCTCACAAAGGCCGGATGgcgc                                                                       >  2:6379405/1‑93 (MQ=255)
                      gCAGGTGGAGCTTATCGAAAAGCTGCTTCTGAGTCAGACGGTCAGCAATAATCGGGAACACATCGCGGTTCACAAAGGCCGGATGGCGCGCCa                                                                   >  1:4822148/1‑93 (MQ=255)
                      gCAGGTGGAGCTTATCGAAAAGCTGCTTCTGAGTCAGACGGTCAGCAATAATCGGGAACACATCGCGGCTCACAAAGGCCGGATGGCGCGCCa                                                                   >  2:4829188/1‑93 (MQ=255)
                      gCAGGTGGAGCTTATCGAAAAGCTGCTTCTGAGTCAGACGGTCAGCAATAATCGGGAACACATCGCGGCTCACAAAGGCCGGATGGCGCGCCa                                                                   <  1:6356163/93‑1 (MQ=255)
                      gCAGGTGGAGCTTATCGAAAAGCTGCTTCTGAGTCAGACGGTCAGCAATAATCGGGAACACATCGCGGCTCACAAAGGCCGGATGGCGCGCCa                                                                   >  2:1943091/1‑93 (MQ=255)
                      gCAGGTGGAGCTTATCAAAAAGCTGCTTCTGAGTCAGACGGTCAGCAATAATCGGGAACACATCGCGGCTCACAAAGGCCGGATGGCGCGCCa                                                                   <  1:6960/93‑1 (MQ=255)
                        aGGTGGAGCTTATCGAAAAGCTGCTTCTGAGTCAGACGGTCAGCAATAATCGGGAACACATCGCGGCTCACAAAGGCCGGATGGCGCGCCCGc                                                                 >  1:5884967/1‑93 (MQ=255)
                         ggTTGAGCTTATCGAAAAGCTGCTTCTGAGTCAGACGGTCAGCAATAATCGGGAACACATCGCCGCTCACAAAGGCCGGATGGCGCGCCAGCt                                                                >  1:3274920/1‑93 (MQ=255)
                         ggTGGAGCTTATCGAAAAGCTGCTTCTGAGTCAGACGGTCAGCAATAATCGGGAACACATCGCGGCTCACAAAGGCCGGATGGCGCGCCAGCt                                                                <  2:5675908/93‑1 (MQ=255)
                         ggTGGAGCTTATCGAAAAGCTGCTTCTGAGTCAGACGGTCAGCAATAATCGGGAACACATCGCGGCTCACAAAGGCCGGATGGCGCGCCAGCt                                                                >  2:1704411/1‑93 (MQ=255)
                         ggTGGAGCTTATCGAAAAGCTGCTTCTGAGTCAGACGGTCAGCAATAATCGGGAACACATCGCGGCTCACAAAGGCCGGATGGCGCGCCAGCt                                                                >  2:3581686/1‑93 (MQ=255)
                          gTGGAGCTTATCGAAAAGCTGCTTCTGAGTCAGACGGTCAGCAATAATCGGGAACACATCGCGGCTCACAAAGGCCGGATGGCGCGCCAGCTc                                                               >  2:5868751/1‑93 (MQ=255)
                           tGGAGCTTATCGAAAAGCTGCTTCTGAGTCAGACGGTCAGCAATAATCGGGAACACATCGCGGCTCACAAAGGCCGGATGGCGCGCCAggccg                                                              >  1:1936405/1‑89 (MQ=255)
                             gAGCTTATCGAAAAGCTGCTTCTGAGTCAGACGGTCAGCAATAATCGGGAACACATCGCGGCTCACAAAGGCCGGATGGCGCGCCAGCTcgcg                                                            <  1:2782353/93‑1 (MQ=255)
                              aGCTTATCGAAAAGCTGCTTCTGAGTCAGACGGTCAGCAATAATCGGGAACACATCGCGGCTCACAAAGGCCGGATGGCGCGCCAGCTCGCgg                                                           <  1:261083/93‑1 (MQ=255)
                               gCTTATCGAAAAGCTGCTTCTGAGTCAGACGGTCAGCAATAATCGGGAACACATCGCGGCTCACAAAGGCCGGATGGCGCGCCAGCTCGCggg                                                          >  2:4199324/1‑93 (MQ=255)
                                cTTATCGAAAAGCTGCTTCTGAGTCAGACGGTCAGCAATAATCGGGAACACATCGCGGCTCACAAAGGCCGGATGGCGCGCCAGCTCGCGGGt                                                         >  1:5134798/1‑93 (MQ=255)
                                 ttATCGAAAAGCTGCTTCTGAGTCAGACGGTCAGCAATAATCGGGAACACATCGCGGCTCACAAAGGCCGGATGGCGCGCCAGCTCGCGGGtt                                                        >  2:6262287/1‑93 (MQ=255)
                                    tCGAAAAGCTGCTTCTGAGTCAGACGGTCAGCAATAATCGGGAACACATCGCGGCTCACAAAGGCCGGATGGCGCGCCAGCTCGCGGGTTAAt                                                     >  2:3901551/1‑93 (MQ=255)
                                     cGAAAAGCTGCTTCTGAGTCAGACGGTCAGCAATAATCGGGAACACATCGCGGCTCACAAAGGCCGGATGGCGCGCCAGCTCGCGGGTTAATg                                                    <  1:3031980/93‑1 (MQ=255)
                                      gAAAAGCTGCTTCTGAGTCAGACGGTCAGCAATAATCGGGAACACATCGCGGCTCACAAAGGCCGGATGGCGCGCCAGCTCGCGGGTTAATGc                                                   >  1:10072/1‑93 (MQ=255)
                                      gAAAAGCTGCTTCTGAGTCAGACGGTCAGCAATAATCGGGAACACATCGCGGCTCACAAAGGCCGGATGGCGCGCCAGCTCGCGGGTTAATGc                                                   >  2:5112571/1‑93 (MQ=255)
                                       aaaaGCTGCTTCTGAGTCAGACGGTCAGCAATAATCGGGAACACATCGCGGCTCACAAAGGCCGGATGGCGCGCCAGCTCGCGGGTTAATGCg                                                  >  1:1758723/1‑93 (MQ=255)
                                        aaaGCTGCTTCTGAGTCAGACGGTCAGCAATAATCGGGAACACATCGCGGCTCACAAAGGCCGGATGGCGCGCCAGCTCGCGGGTTAATGCgg                                                 <  1:4622574/93‑1 (MQ=255)
                                        aaaGCTGCTTCTGAGTCAGACGGTCAGCAATAATCGGGAACACATCGCGGCTCACAAAGGCCGGATGGCGCGCCAGCTCGCGGGTTAATGCgg                                                 <  1:3734452/93‑1 (MQ=255)
                                        aaaGCTGCTTCTGAGTCAGACGGTCAGCAATAATCGGGAACACATCGCGGCTCACAAAGGCCGGATGGCGCGCCAGCTCGCGGGTTAATGCgg                                                 >  2:1090876/1‑93 (MQ=255)
                                         aaGCTGCTTCTGAGTCAGACGGTCAGCAATAATCGGGAACACATCGCGGCTCACAAAGGCCGGATGGCGCGCCAGCTCGCGGGTTAATGCGGt                                                >  1:5251426/1‑93 (MQ=255)
                                          aGCTGCTTCTGAGTCAGACGGTCAGCAATAATCGGGAACACATCGCGGCTCACAAAGGCCGGATGGCGCGCCAGCTCGCGGGTTAATGCGGtt                                               <  2:5420217/93‑1 (MQ=255)
                                            ctgctTCTGAGTCAGACGTTCAGCAATAATCGGGAACACATCGCGGCTCACAAAGGCCGGATGGCGCGCCAGCTCGCGGGTTAATGCGGTTTc                                             >  1:4931799/1‑93 (MQ=255)
                                            ctgctTCTGAGTCAGACGGTCAGCAATAATCGGGAACACATCGCGGCTCACAAAGGCCGGATGGCGCGCCAGCTCGCGGGTTAATGCGGTTTc                                             <  1:4230006/93‑1 (MQ=255)
                                             tgctTCTGAGTCAGACGGTCAGCAATAATCGGGAACACATCGCGGCTCACAAAGGCCGGATGGCGCGCCAGCTCGCGGGTTAATGCGGTTTcc                                            <  1:4503758/93‑1 (MQ=255)
                                              gctTCTGAGTCAGACGGTCAGCAATAATCGGGAACACATCGCGGCTCACAAAGGCCGGATGGCGCGCCAGCTCGCGGGTTAATGCGGTTTCCg                                           >  2:2386230/1‑93 (MQ=255)
                                               ctTCTGAGTCAGACGGTCAGCAATAATCGGGAACACATCGCGGCTCACAAAGGCCGGATGGCGCGCCAGCTCGCGGGTTAATGCGGTTTGCgg                                          >  2:127140/1‑93 (MQ=255)
                                               ctTCTGAGTCAGACGGTCAGCAATAATCGGGAACACATCGCGGCTCACAAAGGCCGGATGGCGCGCCAGCTCGCGGGTTAATGCGGTTTCCgg                                          >  1:3524552/1‑93 (MQ=255)
                                                ttCTGAGTCAGACGGTCAGCAATAATCGGGAACACATCGCGGCTCACAAAGGCCGGATGGCGCGCCAGCTCGCGGGTTAATGCGGTTTCCGGc                                         <  1:2539306/93‑1 (MQ=255)
                                                ttCTGAGTCAGACGGTCAGCAATAATCGGGAACACATCGCGGCTCACAAAGGCCGGATGGCGCGCCAGCTCGCGGGTTAATGCGGTTTCCGGc                                         >  2:131170/1‑93 (MQ=255)
                                                 tCTGAGTCAGACGGTCAGCAATAATCGGGAACACATCGCGGCTCACAAAGGCCGGATGGCGCGCCAGCTCGCGGGTTAATGCGGTTTCCGGcc                                        <  1:61590/93‑1 (MQ=255)
                                                   tGAGTCAGACGGTCAGCAATAATCGGGAACACATCGCGGTGCATAAAGGCCGGATGGCGCGCCAGCGCGCGGGTTAATGCGGGTTCCGGCCAg                                      >  1:6473240/1‑93 (MQ=255)
                                                   tGAGTCAGACGGTCAGCAATAATCGGGAACACATCGCGGCTCACAAAGGCCGGATGGCGCGCCAGCTCGCGGGTTAATGCGGTTTCCGGCCAg                                      >  1:3875582/1‑93 (MQ=255)
                                                    gAGTCAGACGGTCAGCAATAATCGGGAACACATCGCGGCTCACAAAGGCCGGATGGCGCGCCAGCTCGCGGGTTAATGCGGTTTCCGGCCAGc                                     <  2:5742206/93‑1 (MQ=255)
                                                    gAGTCAGACGGTCAGCAATAATCGGGAACACATCGCGGCTCACAAAGGCCGGATGGCGCGCCAGCTCGCGGGATAATGCGGCTTCCCGCCAGc                                     >  1:1496948/1‑93 (MQ=255)
                                                      gTCAGACGGTCAGCAATAATCGGGAACACATCGCGGCTCACAAAGGCCGGATGGCGCGCCAGCTCGCGGGTTAATGCGGTTTCCGGCCAGCGt                                   >  2:3012114/1‑93 (MQ=255)
                                                       tCAGACGGTCAGCAATAATCGGGAACACATCGCGGCTCACAAAGGCCGGATGGCGCGCCAGCTCGCGGGTTAATGCGGTTTCCGGCCAGCGtt                                  <  2:730100/93‑1 (MQ=255)
                                                         aGACGGTCAGCAATAATCGGGAACACATCGCGGCTCACAAAGGCCGGATGGCGCGCCAGCTCGCGGGTTAATGCGGTTTCCGGCCAGCGTTCt                                >  1:1550777/1‑93 (MQ=255)
                                                         aGACGGTCAGCAATAATCGGGAACACATCGCGGCTCACAAAGGCCGGATGGCGCGCCAGCTCGCGGGTTAATGCGGGTTCCGGCCAGCGTTCt                                >  1:3959162/1‑93 (MQ=255)
                                                          gACGGTCAGCAATAATCGGGAACACATCGCGGCTCACAAAGGCCGGATGGCGCGCCAGCTCGCGGGTTAATGCGGTTTCCGGCCAGCGTTCTa                               >  2:65041/1‑93 (MQ=255)
                                                              gTCAGCAATAATCGGGAACACATCGCGGCTCACAAAGGCCGGATGGCGCGCCAGCTCGCGGGTTAATGCGGTTTCCGGCCAGCGTTCTAtctc                           >  1:5196420/1‑93 (MQ=255)
                                                              gTCAGCAATAATCGGGAACACATCGCGGCTCACAAAGGCCGGATGGCGCGCCAGCTCGCGGGTTAATGCGGTTTCCGGCCAGCGTTCTAtctc                           >  1:5435341/1‑93 (MQ=255)
                                                                 aGCAATAATCGGGAACACATCGCGGCTCACAAAGGCCGGATGGCGCGCCAGCTCGCGGGTTAATGCGGTTTCCGGCCAGCGTTCTATCTCAGc                        <  1:1183818/93‑1 (MQ=255)
                                                                 aGCAATAATCGGGAACACATCGCGGCTCACAAAGGCCGGATGGCGCGCCAGCTCGCGGGTTAATGCGGTTTCCGGCCAGCGTTCTATCTCAGc                        <  2:2599536/93‑1 (MQ=255)
                                                                   cAATAATCGGGAACACATCGCGGCTCACAAAGGCCGGATGGCGCGCCAGCTCGCGGGTTAATGCGGTTTCCGGCCAGCGTTCTATCTCAGCgg                      >  2:2625114/1‑93 (MQ=255)
                                                                   cAATAATCGGGAACACATCGCGGCTCACAAAGGCCGGATGGCGCGCCAGCTCGCGGGTTAATGCGGTTTCCGGCCAGCGTTCTATCTCAGCgg                      <  1:2209196/93‑1 (MQ=255)
                                                                   cAATAATCGGGAACACATCGCGGCTCACAAAGGCCGGATGGCGCGCCAGCTCGCGGGTTAATGCGGTTTCCGGCCAGCGTTCTATCTCAGCgg                      <  1:6341044/93‑1 (MQ=255)
                                                                    aataatCGGGAACACATCGCGGCTCACAAAGGCCGGATGGCGCGCCAGCTCGCGGGTTAATGCGGTTTCCGGCCAGCGTTCTATCTCAGCGGt                     >  2:5783909/1‑93 (MQ=255)
                                                                     ataatCGGGAACACATCGCGGCTCACAAAGGCCGGATGGCGCGCCAGCTCGCGGGTTAATGCGGTTTCCGGCCAGCGTTCTATCTCAGCGGTa                    <  2:1433310/93‑1 (MQ=255)
                                                                     ataatCGGGAACACATCGCGGCTCACAAAGGCCGGATGGCGCGCCAGCTCGCGGGTTAATGCGGTTTCCGGCCAGCGCTCTATCTCAGCggga                    >  2:470058/1‑91 (MQ=255)
                                                                        atCGGGAACACATCGCGGCTCACAAAGGCCGGATGGCGCGCCAGCTCGCGGGTTAATGCGGTTTCCGGCCAGCGTTCTATCTCAGCGGTAATc                 >  2:3720066/1‑93 (MQ=255)
                                                                        atCGGGAACACATCGCGGCTCACAAAGGCCGGATGGCGCGCCAGCTCGCGGGTTAATGCGGTTTCCGGCCAGCGTTCTATCTCAGCGGTAATc                 <  1:1732426/93‑1 (MQ=255)
                                                                         tCGGGAACACATCGCGGCTCACAAAGGCCGGATGGCGCGCCAGCTCGCGGGTTAATGCGGTTTCCGGCCAGCGTTCTATCTCAGCGGTAATCa                <  1:2057855/93‑1 (MQ=255)
                                                                         tCGGGAACACATCGCGGCTCACAAAGGCCGGATGGCGCGCCAGCTCGCGGGTTAATGCGGTTTCCGGCCAGCGTTCTATCTCAGCGGTAATCa                >  2:6005141/1‑93 (MQ=255)
                                                                         tCGGGAACACATCGCGGCTCACAAAGGCCGGATGGCGCGCCAGCTCGCGGGTTAATGCGGTTTCCGGCCAGCGTTCTATCTCAGCGGTAATCa                >  1:5552248/1‑93 (MQ=255)
                                                                         tCGGGAACACATCGCGGCTCACAAAGGCCGGATGGCGCGCCAGCTCGCGGGTTAATGCGGTTTCCGGCCAGCGTTCTATCTCAGCGGTAATCa                <  1:1265187/93‑1 (MQ=255)
                                                                         tCGGGAACACATCGCGGCTCACAAAGGCCGGATGGCGCGCCAGCTCGCGGGTTAATGCGGTTTCCGGCCAGCGTTCTATCTCAGCGGTAATCa                <  2:2480328/93‑1 (MQ=255)
                                                                          cGGGAACACATCGCGGCTCACAAAGGCCGGATGGCGCGCCAGCTCGCGGGTTAATGCGGTTTCCGGCCAGCGTTCTATCTCAGCGGTAATCAc               <  2:4464165/93‑1 (MQ=255)
                                                                           gggAACACATCGCGGCTCACAAAGGCCGGATGGCGCGCCAGCTCGCGGGTTAATGCGGTTTCCGGCCAGCGTTCTATCTCAGCGGTAATCACg              >  1:6009606/1‑93 (MQ=255)
                                                                           gggAACACATCGCGGCTCACAAAGGCCGGATGGCGCGCCAGCTCGCGGGTTAATGCGGTTTCCGGCCAGCGTTCTATCTCAGCGGTAATCACg              >  1:2366170/1‑93 (MQ=255)
                                                                           gggAACACATCGCGGCTCACAAAGGCCGGATGGCGCGCCAGCTCGCGGGTTAATGCGGTTTCCGGCCAGCGTTCTATCTCAGCGGTAATCACg              >  2:450536/1‑93 (MQ=255)
                                                                           gggAACACATCGCGGCTCACAAAGGCCGGATGGCGCGCCAGCTCGCGGGTTAATGCGGTTTCCGGCCAGCGTTCTATCTCAGCGGTAATCACg              >  2:2046971/1‑93 (MQ=255)
                                                                            ggAACACATCGCGGCTCACAAAGGCCGGATGGCGCGCCAGCTCGCGGGTTAATGCGGTTTCCGGCCAGCGTTCTATCTCCGCGGTAATCACGc             >  1:3156571/1‑93 (MQ=255)
                                                                             gAACACATCGCGGCTCACAAAGGCCGGATGGCGCGCCAGCTCGCGGGTTAATGCGGTTTCCGGCCAGCGTTCTATCTCAGCGGTAATCACGCt            >  1:2967157/1‑93 (MQ=255)
                                                                              aaCACATCGCGGCTCACAAAGGCCGGATGGCGCGCCAGCTCGCGGGTTAATGCGGTTTCCGGCCAGCGTTCTATCTCAGCGGTAATCACGCtt           <  1:4456501/93‑1 (MQ=255)
                                                                              aaCACATCGCGGCTCACAAAGGCCGGATGGCGCGCCAGCTCGCGGGTTAATGCGGTTTCCGGCCAGCGTTCTATCTCAGCGGTAATCACGCtt           <  2:685001/93‑1 (MQ=255)
                                                                               acacATCGCGGCTCACAAAGGCCGGATGGCGCGCCAGCTCGCGGGTTAATGCGGTTTCCGGCCAGCGTTCTATCTCAGCGGTAATCACGCttt          <  1:2466039/93‑1 (MQ=255)
                                                                               acacATCGCGGCTCACAAAGGCCGGATGGCGCGCCAGCTCGCGGGTTAATGCGGTTTCCGGCCAGCGTTCTATCTCAGCGGTAATCACGCttt          <  2:2121514/93‑1 (MQ=255)
                                                                               acacATCGCGGCTCACAAAGGCCGGATGGCGCGCCAGCTCGCGGGTTAATGCGGTTTCCGGCCAGCGTTCTATCTCAGCGGTAATCACGCttt          <  1:2234981/93‑1 (MQ=255)
                                                                               acacATCGCGGCTCACAAAGGCCGGATGGCGCGCCAGCTCGCGGGTTAATGCGGTTTCCGGCCAGCGTTCTATCTCAGCGGTAATCACGCttt          >  1:2107504/1‑93 (MQ=255)
                                                                                cacaTCGCGGCTCACAAAGGCCGGATGGCGCGCCAGCTCGCGGGTTAATGCGGTTTCCGGCCAGCGTTCTATCTCAGCGGTAATCACGCTttg         <  1:4720777/93‑1 (MQ=255)
                                                                                cacaTCGCGGCTCACAAAGGCCGGATGGCGCGCCAGCTCGCGGGTTAATGCGGTTTCCGGCCAGCGTTCTATCTCAGCGGTAATCACGCTttg         <  2:2800845/93‑1 (MQ=255)
                                                                                 acaTCGCGGCTCACAAAGGCCGGATGGCGCGCCAGCTCGCGGGTTAATGCGGTTTCCGGCCAGCGTTCTATCTCAGCGGTAATCACGCTttgt        >  1:5929287/1‑93 (MQ=255)
                                                                                   aTCGCGGCTCACAAAGGCCGGATGGCGCGCCAGCTCGCGGGTTAATGCGGTTTCCGGCCAGCGTTCTATCTCAGCGGTAATCACGCTttgttg      >  1:5051788/1‑93 (MQ=255)
                                                                                     cgcgGCTCACAAAGGCCGGATGGCGCGCCAGCTCGCGGGTTAATGCGGTTTCCGGCCAGCGTTCTATCTCAGCGGTAATCACGCTTTGTTGaa    <  2:809037/93‑1 (MQ=255)
                                                                                      gcgGCTCACAAAGGCCGGATGGCGCGCCAGCTCGCGGGTTAATGCGGTTTCCGGCCAGCGTTCTATCTCAGCGGTAATCACGCTTTGTTGaaa   >  1:1401724/1‑93 (MQ=255)
                                                                                          |                                                                                         
AGTAACTGCCACGGTGCAGTCGGCAGGTGGAGCTTATCGAAAAGCTGCTTCTGAGTCAGACGGTCAGCAATAATCGGGAACACATCGCGGTTCACAAAGGCCGGATGGCGCGCCAGCTCGCGGGTTAATGCGGTTTCCGGCCAGCGTTCTATCTCAGCGGTAATCACGCTTTGTTGAAAA  >  NC_000913/551519‑551698

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 19 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
GCAAGTAACTGCCACGGTGCAGTCGGCAGGTGGAGCTTATCGAAAAGCTGCTTCTGAGTCAGACGGTCAGCAATAATCGGGAACACATCGCGGTTCACAAAGGCCGGATGGCGCGCCAGCTCGCGGGTTAATGCGGTTTCCGGCCAGCGTTCTATCTCAGCGGTAATCACGCTTTGTTGAAAAGGCA  >  NC_000913/551516‑551702
                                                                                             |                                                                                             
GCAAGTAACTGCCACGGTGCAGTCGGCAGGTGGAGCTTATCGAAAAGCTGCTTCTGAGTCAGACGGTCAGCAATAATCGGGAACACATCGCGGC                                                                                               >  SRR4302537.5064252/1‑94 (MQ=60)
 CAAGTAACTGCCACGGTGCAGTCGGCAGGTGGAGCTTATCTAAAAGCTGCTTCTGAGTCAGACGGTCAGCAATAATCGGGAACACATCGCGGCT                                                                                              >  SRR4302537.2538069/1‑94 (MQ=60)
 CAAGTAACTGCCACGGTGCAGTCGGCAGGTGGAGCTTATCGAAAAGCTGCTTCTGAGTCAGACGGTCAGCAATAATCGGGAACACATCGCGGCT                                                                                              >  SRR4302537.6321535/1‑94 (MQ=60)
 CAAGTAACTGCCACGGTGCAGTCGGCAGGTGGAGCTTATCGAAAAGCTGCTTCTGAGTCAGACGGTCAGCAATAATCGGGAACACATCGCGGCT                                                                                              <  SRR4302537.3406568/94‑1 (MQ=60)
 CAAGTAACTGCCACGGTGCAGTCGGCAGGTGGAGCTTATCGAAAAGCTGCTTCTGAGTCAGACGGTCAGCAATAATCGGGAACACATCGCGGCT                                                                                              <  SRR4302537.6265832/94‑1 (MQ=60)
   AGTAACTGCCACGGTGCAGTCGGCAGGTGGAGCTTATCGAAAAGCTGCTTCTGAGTCAGACGGTCAGCAATAATCGGGAACACATCGCGGCTCA                                                                                            <  SRR4302537.5538825/94‑1 (MQ=60)
   AGTAACTGCCACGGTGCAGTCGGCAGGTGGAGCTTATCGAAAAGCTGCTTCTGAGTCAGACGGTCAGCAATAATCGGGAACACATCGCGGCTCA                                                                                            <  SRR4302537.2054173/94‑1 (MQ=60)
   AGTAACTGCCACGGTGCAGTCGGCAGGTGGAGCTTATCGAAAAGCTGCTTCTGAGTCAGACGGTCAGCAATAATCGGGAACACATCGCGGCTCA                                                                                            <  SRR4302537.818230/94‑1 (MQ=60)
       ACTGCCACGGTGCAGTCGGCAGGTGGAGCTTATCGAAAAGCTGCTTCTGAGTCAGACGGTCAGCAATAATCGGGAACACATCGCGGCTCACAAA                                                                                        >  SRR4302537.6473026/1‑94 (MQ=60)
        CTGCCACGGTGCAGTCGGCAGGTGGAGCTTATCGAAAAGCTGCTTCTGAGTCATACGGTCAGCAATAATCGGGAACACATCGCGGTTCACAAAG                                                                                       <  SRR4302537.4701874/94‑1 (MQ=60)
        CTGCCACGGTGCAGTCGGCAGGTGGAGCTTATCGAAAAGCTGCTTCTGAGTCAGACGGTCAGCAATAATCGGGAACACATCGCGGCTCACAAAG                                                                                       >  SRR4302537.2436802/1‑94 (MQ=60)
        CTGCCACGGTGCAGTCGGCAGGTGGAGCTTATCGAAAAGCTGCTTCTGAGTCAGACGGTCAGCAATAATCGGGAACACATCGCGGCTCACAAAG                                                                                       <  SRR4302537.2443437/94‑1 (MQ=60)
        CTGCCACGGTGCAGTCGGCAGGTGGAGCTTATCGAAAAGCTGCTTCTGAGTCAGACGGTCAGCAATAATCGGGAACACATCGCGGCTCACAAAG                                                                                       >  SRR4302537.2095794/1‑94 (MQ=60)
         TGCCACGGTGCAGTCGGCAGGTGGAGCTTATCGAAAAGCTGCTTCTGAGTCAGACGGTCAGCAATAATCGGGAACACATCGCGGCTCACAAAGG                                                                                      >  SRR4302537.359997/1‑94 (MQ=60)
         TGCCACGGTGCAGTCGGCAGGTGGAGCTTATCGAAAAGCTGCTTCTGAGTCAGACGGTCAGCAATAATCGGGAACACATCGCGGCTCACAAAGG                                                                                      <  SRR4302537.3831216/94‑1 (MQ=60)
          GCCACGGTGCAGTCGGCAGGTGGAGCTTATCGAAAAGCTGCTTCTGAGTCAGACGGTCAGCAATAATCGGGAACACATCGCGGCTCACAAAGGC                                                                                     <  SRR4302537.1260962/94‑1 (MQ=60)
          GCCACGGTGCAGTCGGCAGGTGGAGCTTATCGAAAAGCTGCTTCTGAGTCAGACGGTCAGCAATAATCGGGAACACATCGCGGCTCACAAAGGC                                                                                     <  SRR4302537.3753961/94‑1 (MQ=60)
           CCACGGTGCAGTCGGCAGGTGGAGCTTATCGAAAAGCTGCTTCTGAGTCAGACGGTCAGCAATAATCGGGAACACATCGCGGCTCACAAAGGCC                                                                                    <  SRR4302537.634145/94‑1 (MQ=60)
           CCACGGTGCAGTCGGCAGGTGGAGCTTATCGAAAAGCTGCTTCTGAGTCAGACGGTCAGCAATAATCGGGAACACATCGCGGCTCACAAAGGCC                                                                                    <  SRR4302537.5338299/94‑1 (MQ=60)
            CACGGTGCAGTCGGCAGGTGGAGCTTATCGAAAAGCTGCTTCTGAGTCAGACGGTCAGCAATAATCGGGAACACATCGCGGCTCACAAAGGCCG                                                                                   <  SRR4302537.5974216/94‑1 (MQ=60)
            CACGGTGCAGTCGGCAGGTGGAGCTTATCGAAAAGCTGCTTCTGAGTCAGACGGTCAGCAATAATCGGGAACACATCGCGGCTCACAAAGGCCG                                                                                   <  SRR4302537.2291173/94‑1 (MQ=60)
             ACGGTGCAGTCGGCAGGTGGAGCTTATCGAAAAGCTGCTTCTGAGTCAGACGGTCAGCAATAATCGGGAACACATCGCGGCTCACAAAGGCCGG                                                                                  >  SRR4302537.3197062/1‑94 (MQ=60)
             ACGGTGCAGTCGGCAGGTGGAGCTTATCGAAAAGCTGCTTCTGAGTCAGACGGTCAGCAATAATCGGGAACACATCGCGGCTCACAAAGGCCGG                                                                                  >  SRR4302537.3842559/1‑94 (MQ=60)
                GTGCAGTCGGCAGGTGGAGCTTATCGAAAAGCTGCTTCTGAGTCAGACGGTCAGCAATAATCGGGAACACATCGCGGCTCACAAAGGCCGGATG                                                                               <  SRR4302537.1395613/94‑1 (MQ=60)
                  GCAGTCGGCAGGTGGAGCTTATCGAAAAGCTGCTTCTGAGTCAGACGGTCAGCAATAATCGGGAACACATCGCGGCTCACAAAGGCCGGATGGC                                                                             <  SRR4302537.4293505/94‑1 (MQ=60)
                   CAGTCGGCAGGTGGAGCTTATCGAAAAGCTGCTTCTGAGTCAGACGGTCAGCAATAATCGGGAACACATCGCGGCTCACAAAGGCCGGATGGCG                                                                            <  SRR4302537.1767677/94‑1 (MQ=60)
                    AGTCGGCAGGTGGAGCTTATCGAAAAGCTGCTTCTGAGTCAGACGGTCAGCAATAATCGGGAACACATCGCGGCTCACAAAGGCCGGATGGCGC                                                                           >  SRR4302537.4312639/1‑94 (MQ=60)
                     GTCGGCAGGTGGAGCTTATCGAAAAGCTGCTTCTGAGTCAGACGGTCAGCAATAATCGGGAACACATCGCGGCTCACAAAGGCCGGATGGCGCG                                                                          <  SRR4302537.681708/94‑1 (MQ=60)
                        GGCAGGTGGAGCTTATCGAAAAGCTGCTTCTGAGTCAGACGGTCAGCAATAATCGGGAACACATCGCGGTTCACAAAGGCCGGATGGCGCGCCA                                                                       >  SRR4302537.4855456/1‑94 (MQ=60)
                         GCAGGTGGAGCTTATCGAAAAGCTGCTTCTGAGTCAGACGGTCAGCAATAATCGGGAACACATCGCGGCTCACAAAGGCCGGATGGCGCGCCAG                                                                      >  SRR4302537.3557051/1‑94 (MQ=60)
                         GCAGGTGGAGCTTATCGAAAAGCTGCTTCTGAGTCAGACGGTCAGCAATAATCGGGAACACATCGCGGCTCACAAAGGCCGGATGGCGCGCCAG                                                                      <  SRR4302537.6399546/94‑1 (MQ=60)
                         GCAGGTGGAGCTTATCGAAAAGCTGCTTCTGAGTCAGACGGTCAGCAATAATCGGGAACACATCGCGGCTCACAAAGGCCGGATGGCGCGCCAG                                                                      >  SRR4302537.5042243/1‑94 (MQ=60)
                         GCAGGTGGAGCTTATCAAAAAGCTGCTTCTGAGTCAGACGGTCAGCAATAATCGGGAACACATCGCGGCTCACAAAGGCCGGATGGCGCGCCAG                                                                      <  SRR4302537.7011/94‑1 (MQ=60)
                          CAGGTGGAGCTTATCGAAAAGCTGCTTCTGAGTCAGACGGTCAGCAATAATCGGGAACACATCGCGGCTCACAAAGGCCGGATGGCGCGCCCGC                                                                     >  SRR4302537.5925349/1‑94 (MQ=60)
                          CAGGTGGAGCTTATCGAAAAGCTGCTTCTGAGTCAGACGGTCAGCAATAATCGGGAACACATCGCGGCTCACAAAGGCCGGATGGCGCGCCAGC                                                                     <  SRR4302537.5576430/94‑1 (MQ=60)
                           AGGTTGAGCTTATCGAAAAGCTGCTTCTGAGTCAGACGGTCAGCAATAATCGGGAACACATCGCCGCTCACAAAGGCCGGATGGCGCGCCAGCT                                                                    >  SRR4302537.3298029/1‑94 (MQ=60)
                             GTGGAGCTTATCGAAAAGCTGCTTCTGAGTCAGACGGTCAGCAATAATCGGTAACACATCGCGGCTCACAAAGGCCGGATGGCGCGCCAGCTCG                                                                  <  SRR4302537.4864003/94‑1 (MQ=60)
                             GTGGAGCTTATCGAAAAGCTGCTTCTGAGTCAGACGGTCAGCAATAATCGGGAACACATCGCGGCTCACAAAGGCCGGATGGCGCGCCAGgccg                                                                  >  SRR4302537.1949869/1‑90 (MQ=60)
                             GTGGAGCTTATCGAAAAGCTGCTTCTGAGTCAGACGGTCAGCAATAATCGGGAACACATCGCGGCTCACAAAGGCCGGATGGCGCGCCAGCTCG                                                                  >  SRR4302537.1443282/1‑94 (MQ=60)
                             GTGGAGCTTATCGAAAAGCTGCTTCTGAGTCAGACGGTCAGCAATAATCGGGAACACATCGCGGCTCACAAAGGCCGGATGGCGCGCCAGCTCG                                                                  <  SRR4302537.5673142/94‑1 (MQ=60)
                              TGGAGCTTATCGAAAAGCTGCTTCTGAGTCAGACGGTCAGCAATAATCGGGAACACATCGCGGCTCACAAAGGCCGGATGGCGCGCCAGCTCGC                                                                 >  SRR4302537.1771818/1‑94 (MQ=60)
                                GAGCTTATCGAAAAGCTGCTTCTGAGTCAGACGGTCAGCAATAATCGGGAACACATCGCGGCTCACAAAGGCCGGATGGCGCGCCAGCTCGCGG                                                               <  SRR4302537.2801802/94‑1 (MQ=60)
                                GAGCTTATCGAAAAGCTGCTTCTGAGTCAGACGGTCAGCAATAATCGGGAACACATCGCGGCTCACAAAGGCCGGATGGCGCGCCAGCTCGCGG                                                               <  SRR4302537.3002404/94‑1 (MQ=60)
                                 AGCTTATCGAAAAGCTGCTTCTGAGTCAGACGGTCAGCAATAATCGGGAACACATCGCGGCTCACAAAGGCCGGATGGCGCGCCAGCTCGCGGG                                                              >  SRR4302537.403477/1‑94 (MQ=60)
                                 AGCTTATCGAAAAGCTGCTTCTGAGTCAGACGGTCAGCAATAATCGGGAACACATCGCGGCTCACAAAGGCCGGATGGCGCGCCAGCTCGCGGG                                                              <  SRR4302537.262923/94‑1 (MQ=60)
                                 AGCTTATCGAAAAGCTGCTTCTGAGTCAGACGGTCAGCAATAATCGGGAACACATAGCGGCTCACAAAGGCCGGATGGCGCGCCAGCTCGCGGG                                                              <  SRR4302537.2213505/94‑1 (MQ=60)
                                  GCTTATCGAAAAGCTGCTTCTGAGTCAGACGGTCAGCAATAATCGGGAACACATCGCGGCTCACAAAGGCCGGATGGCGCGCCAGCTCGCGGGT                                                             >  SRR4302537.5170179/1‑94 (MQ=60)
                                    TTATCGAAAAGCTGCTTCTGAGTCAGACGGTCAGCAATAATCGGGAACACATCGCGGCTCACAAAGGCCGGATGGCGCGCCAGCTCGCGGGTTA                                                           >  SRR4302537.283806/1‑94 (MQ=60)
                                    TTATCGAAAAGCTGCTTCTGAGTCAGACGGTCAGCAATAATCGGGAACACATCGCGGCTCACAAAGGCCGGATGGCGCGCCAGCTCGCGGGTTA                                                           >  SRR4302537.2211899/1‑94 (MQ=60)
                                      ATCGAAAAGCTGCTTCTGAGTCAGACGGTCAGCAATAATCGGGAACACATCGCGGCTCACAAAGGCCGGATGGCGCGCCAGCTCGCGGGTTAAT                                                         <  SRR4302537.1490631/94‑1 (MQ=60)
                                        CGAAAAGCTGCTTCTGAGTCAGACGGTCAGCAATAATCGGGAACACATCGCGGCTCACAAAGGCCGGATGGCGCGCCAGCTCGCGGGTTAATGC                                                       <  SRR4302537.3053116/94‑1 (MQ=60)
                                        CGAAAAGCTGCTTCTGAGTCAGACGGTCAGCAATAATCGGGAACACATCGCGGCTCACAAAGGCCGGATGGCGCGCCAGCTCGCGGGTTAATGC                                                       >  SRR4302537.3106226/1‑94 (MQ=60)
                                        CGAAAAGCTGCTTCTGAGTCAGACGGTCAGCAATAATCGGGAACACATCGCGGCTCACAAAGGCCGGATGGCGCGCCAGCTCGCGGGTTAATGC                                                       >  SRR4302537.10147/1‑94 (MQ=60)
                                         GAAAAGCTGCTTCTGAGTCAGACGGTCAGCAATAATCGGGAACACATCGCGGCTCACAAAGGCCGGATGGCGCGCCAGCTCGCGGGTTAATGCG                                                      >  SRR4302537.1770981/1‑94 (MQ=60)
                                          AAAAGCTGCTTCTGAGTCAGACGGTCAGCAATAATCGGGAACACATCGCGGCTCACAAAGGCCGGATGGCGCGCCAGCTCGCGGGTTAATGCGG                                                     <  SRR4302537.1185627/94‑1 (MQ=60)
                                           AAAGCTGCTTCTGAGTCAGACGGTCAGCAATAATCGGGAACACATCGCGGCTCACAAAGGCCGGATGGCGCGCCAGCTCGCGGGTTAATGCGGT                                                    <  SRR4302537.3760682/94‑1 (MQ=60)
                                           AAAGCTGCTTCTGAGTCAGACGGTCAGCAATAATCGGGAACACATCGCGGCTCACAAAGGCCGGATGGCGCGCCAGCTCGCGGGTTAATGCGGT                                                    <  SRR4302537.4654572/94‑1 (MQ=60)
                                           AAAGCTGCTTCTGAGTCAGACGGTCAGCAATAATCGGGAACACATCGCGGCTCACAAAGGCCGGATGGCGCGCCAGCTCGCGGGTTAATGCGGT                                                    >  SRR4302537.5287562/1‑94 (MQ=60)
                                           AAAGCTGCTTCTGAGTCAGACGGTCAGCAATAATCGGGAACACATCGCGGCTCACAAAGGCCGGATGGCGCGCCAGCTCGCGGGTTAATGCGGT                                                    >  SRR4302537.6322327/1‑94 (MQ=60)
                                            AAGCTGCTTCTGAGTCAGACGGTCAGCAATAATCGGGAACACATCGCGGCTCACAAAGGCCGGATGGCGCGCCAGCTCGCGGGTTAATGCGGTT                                                   <  SRR4302537.5112170/94‑1 (MQ=60)
                                              GCTGCTTCTGAGTCAGACGTTCAGCAATAATCGGGAACACATCGCGGCTCACAAAGGCCGGATGGCGCGCCAGCTCGCGGGTTAATGCGGTTTC                                                 >  SRR4302537.4965790/1‑94 (MQ=60)
                                              GCTGCTTCTGAGTCAGACGGTCAGCAATAATCGGGAACACATCGCGGCTCACAAAGGCCGGATGGCGCGCCAGCTCGCGGGTTAATGCGGTTTC                                                 <  SRR4302537.2712652/94‑1 (MQ=60)
                                              GCTGCTTCTGAGTCAGACGGTCAGCAATAATCGGGAACACATCGCGGCTCACAAAGGCCGGATGGCGCGCCAGCTCGCGGGTTAATGCGGTTTC                                                 >  SRR4302537.200300/1‑94 (MQ=60)
                                               CTGCTTCTGAGTCAGACGGTCAGCAATAATCGGGAACACATCGCGGCTCACAAAGGCCGGATGGCGCGCCAGCTCGCGGGTTAATGCGGTTTCC                                                <  SRR4302537.4259454/94‑1 (MQ=60)
                                               CTGCTTCTGAGTCAGACGGTCAGCAATAATCGGGAACACATCGCGGCTCACAAAGGCCGGATGGCGCGCCAGCTCGCGGGTTAATGCGGTTTCC                                                >  SRR4302537.1660656/1‑94 (MQ=60)
                                               CTGCCTCTGAGTCAGACGGGCAGCAATAAGCGGGAACACATCGCGGCTCACAAAGGCCGGATGGCGCGCCAGCTCGCGGGTTAATGCGGTTTCC                                                <  SRR4302537.896545/94‑1 (MQ=60)
                                                TGCTTCTGAGTCAGACGGTCAGCAATAATCGGGAACACATCGCGGCTCACAAAGGCCGGATGGCGCGCCAGCTCGCGGGTTAATGCGGTTTCCG                                               <  SRR4302537.4534901/94‑1 (MQ=60)
                                                TGCTTCTGAGTCAGACGGTCAGCAATAATCGGGAACACATCGCGGCTCACAAAGGCCGGATGGCGCGCCAGCTCGCGGGTTAATGCGGTTTCCG                                               >  SRR4302537.5473454/1‑94 (MQ=60)
                                                TGCTTCTGAGTCAGACGGTCAGCAATAATCGGGAACACATCGCGGCTCACAAAGGCCGGATGGCGCGCCAGCTCGCGGGTTAATGCGGTTTCCG                                               <  SRR4302537.663624/94‑1 (MQ=60)
                                                 GCTTCTGAGTCAGACGGTCAGCAATAATCGGGAACACATCGCGGCTCACAAAGGCCGGATGGCGCGCCAGCTCGCGGGTTAATGCGGTTTCCGG                                              >  SRR4302537.3549484/1‑94 (MQ=60)
                                                 GCTTCTGAGTCAGACGGTCAGCAATAATCGGGAACACATCGCGGCTCACAAAGGCCGGATGGCGCGCCAGCTCGCGGGTTAATGCGGTTTCCGG                                              >  SRR4302537.6393481/1‑94 (MQ=60)
                                                 GCTTCTGAGTCAGACGGTCAGCAATAATCGGGAACACATCGCGGCTCACAAAGGCCGGATGGCGCGCCAGCTCGCGGGTTAATGCGGTTTCCGG                                              <  SRR4302537.3864964/94‑1 (MQ=60)
                                                 GCGTCAGAGTCAGGCGGTCAGTAATAATCGGGAACACATCGCGGTTCACAAAGGCCGGATGTCGCGCCAGCTCGCGGGTTAATGCGGTTTCCGG                                              <  SRR4302537.73481/94‑1 (MQ=60)
                                                  CTTCTGAGTCAGACGGTCAGCAATAATCGGGAACACATCGCGGCTCACAAAGGCCGGATGGCGCGCCAGCTCGCGGGTTAATGCGGTTTCCGGC                                             >  SRR4302537.5253174/1‑94 (MQ=60)
                                                  CTTCTGAGTCAGACGGTCAGCAATAATCGGGAACACATCGCGGCTCACAAAGGCCGGATGGCGCGCCAGCTCGCGGGTTAATGCGGTTTCCGGC                                             >  SRR4302537.4297723/1‑94 (MQ=60)
                                                   TTCTGAGTCAGACGGTCAGCAATAATCGGGAACACATCGCGGCTCACAAAGGCCGGATGGCGCGCCAGCTCGCGGGTTAATGCGGTTTCCGGCC                                            <  SRR4302537.2557115/94‑1 (MQ=60)
                                                    TCTGAGTCAGACGGTCAGCAATAATCGGGAACACATCGCGGCTCACAAAGGCCGGATGGCGCGCCAGCTCGCGGGTTAATGCGGTTTCCGGCCA                                           <  SRR4302537.62049/94‑1 (MQ=60)
                                                     CTGAGTCAGACGGTCAGCAATAATCGGGAACTCATCGCGGCTCACAAAGGCCGGATGGCGCGCCAGCTCGCGGGTTAATGCGGTTTCCGGCCAG                                          <  SRR4302537.1538452/94‑1 (MQ=60)
                                                     CTGAGTCAGACGGTCAGCAATAATCGGGAACACATCGCGGTGCATAAAGGCCGGATGGCGCGCCAGCGCGCGGGTTAATGCGGGTTCCGGCCAG                                          >  SRR4302537.6517163/1‑94 (MQ=60)
                                                     CTGAGTCAGACGGTCAGCAATAATCGGGAACACATCGCGGCTCACAAAGGCCGGATGGCGCGCCAGCTCGCGGGTTAATGCGGTTTCCGGCCAG                                          >  SRR4302537.3902692/1‑94 (MQ=60)
                                                     CTGAGTCAGACGGTCAGCAATAATCGGGAACACATCGCGGCTCACAAAGGCCGGATGGCGCGCCAGCTCGCGGGTTAATGCGGTTTCCGGCCAG                                          >  SRR4302537.3670661/1‑94 (MQ=60)
                                                      TGAGTCAGACGGTCAGCAATAATCGGGAACACATCGCGGCTCACAAAGGCCGGATGGCGCGCCAGCTCGCGGGTTAATGCGGTTTCCGGCCAGC                                         <  SRR4302537.5061143/94‑1 (MQ=60)
                                                      TGAGTCAGACGGTCAGCAATAATCGGGAACACATCGCGGCTCACAAAGGCCGGATGGCGCGCCAGCTCGCGGGTTAATGCGGTTTCCGGCCAGC                                         >  SRR4302537.3400332/1‑94 (MQ=60)
                                                      TGAGTCAGACGGTCAGCAATAATCGGGAACACATCGCGGCTCACAAAGGCCGGATGGCGCGCCAGCTCGCGGGATAATGCGGCTTCCCGCCAGC                                         >  SRR4302537.1507390/1‑94 (MQ=60)
                                                         GTCAGACGGTCAGCAATAGTCGGGAACACATCGCGGCTCACAAAGGCCGGATGGCGCGCCAGCTCGCGGGTTAATGCGGTTTCCGGCCAGCGTT                                      <  SRR4302537.6463587/94‑1 (MQ=60)
                                                          TCAGACGGTCAGCAATAATCGGGAACACATCGCGGCTCACAAAGGCCGGATGGCGCGCCAGCTCGCGGGTTAATGCGGTTTCCGGCCAGCGTTC                                     <  SRR4302537.2770418/94‑1 (MQ=60)
                                                           CAGACGGTCAGCAATAATCGGGAACACATCGCGGCTGACAAAGGGCGGATGGCGCGACAGCTCGCGGGTTAATGCGGTTTCCGGCCAGCGGTCT                                    >  SRR4302537.1922163/1‑94 (MQ=60)
                                                           CAGACGGTCAGCAATAATCGGGAACACATCGCGGCTCACAAAGGCCGGATGGCGCGCCAGCTCGCGGGTTAATGCGGTTTCCGGCCAGCGTTCT                                    >  SRR4302537.3921310/1‑94 (MQ=60)
                                                           CAGACGGTCAGCAATAATCGGGAACACATCGCGGCTCACAAAGGCCGGATGGCGCGCCAGCTCGCGGGTTAATGCGGTTTCCGGCCAGCGTTCT                                    <  SRR4302537.1642153/94‑1 (MQ=60)
                                                           CAGACGGTCAGCAATAATCGGGAACACATCGCGGCTCACAAAGGCCGGATGGCGCGCCAGCTCGCGGGTTAATGCGGTTTCCGGCCAGCGTTCT                                    >  SRR4302537.1561598/1‑94 (MQ=60)
                                                           CAGACGGTCAGCAATAATCGGGAACACATCGCGGCTCACAAAGGCCGGATGGCGCGCCAGCTCGCGGGTTAATGCGGGTTCCGGCCAGCGTTCT                                    >  SRR4302537.3986865/1‑94 (MQ=60)
                                                            AGACGGTCAGCAATAATAGGGAACACATCGCGGCTCACAAAGGCCGGATGGCGCGCCAGCTCGCGGGTTAATGCGGTTTCCGGCCAGCGTTCTA                                   <  SRR4302537.760275/94‑1 (MQ=60)
                                                             GACGGTCAGCAATAATCGGGAACACATCGCGGCTCACAAAGGCCGGATGGCGCGCCAGCTCGCGGGTTAATGCGGTTTCCGGCCAGCGTTCTAT                                  >  SRR4302537.539021/1‑94 (MQ=60)
                                                             GACGGTCAGCAATAATCGGGAACACATCGCGGCTCACAAAGGCCGGATGGCGCGCCAGCTCGCGGGTTAATGCGGTTTCCGGCCAGCGTTCTAT                                  <  SRR4302537.2275241/94‑1 (MQ=60)
                                                             GACGGTCAGCAATAATCGGGAACACATCGCGGCTCACAAAGGCCGGATGGCGCGCCAGCTCGCGGGTTAATGCGGTTTCCGGCCAGCGTTCTAT                                  >  SRR4302537.674294/1‑94 (MQ=60)
                                                               CGGTCAGCAATAATCGGGAACACATCGCGGCTCACAAAGGCCGGATGGCGCGCCAGCTCGCGGGTTAATGCGGTTTCCGGCCAGCGTTCTATCT                                >  SRR4302537.2996659/1‑94 (MQ=60)
                                                                GGTCAGCAATAATCGGGAACACATCGCGGCTCACAAAGGCCGGATGGCGCGCCAGCTCGCGGGTTAATGCGGTTTCCGGCCAGCGTTCTATCTC                               >  SRR4302537.5232138/1‑94 (MQ=60)
                                                                GGTCAGCAATAATCGGGAACACATCGCGGCTCACAAAGGCCGGATGGCGCGCCAGCTCGCGGGTTAATGCGGTTTCCGGCCAGCGTTCTATCTC                               >  SRR4302537.5472417/1‑94 (MQ=60)
                                                                 GTCAGCAATAATCGGGAACACATCGCGGCTCACAAAGGCCGGATGGCGCGCCAGCTCGCGGGTTAATGCGGTTTCCGGCCAGCGTTCTATCTCA                              <  SRR4302537.596952/94‑1 (MQ=60)
                                                                 GTCAGCAATAATCGGGAACACATCGCGGCTCACAAAGGCCGGATGGCGCGCCAGCTCGCGGGTTAATGCGGTTTCCGGCCAGCGTTCTATCTCA                              <  SRR4302537.249261/94‑1 (MQ=60)
                                                                 GTCAGCAATAATCGGGAACACATCGCGGCTCACAAAGGCCGGATGGCGCGCCAGCTCGCGGGTTAATGCGGTTTCCGGCCAGCGTTCTATCTCA                              >  SRR4302537.3855774/1‑94 (MQ=60)
                                                                 GTCAGCAATAATCGGGAACACATCGCGGCTCACAAAGGCCGGATGGCGCGCCAGCTCGCGGGTTAATGCGGTTTCCGGCCAGCGTTCTATCTCA                              <  SRR4302537.4512758/94‑1 (MQ=60)
                                                                   CAGTAATAATCGGAAACACATCGCAACTCGCAAAGGCCGGATGGCGCTCCAGCTCGCGGGTTAATGCGGTTTCCTGCCAGCGTTCTATCTCAGC                            <  SRR4302537.84971/94‑1 (MQ=60)
                                                                    AGCAATAATCGGGAACACATCGCGGCTCACAAAGGCCGGATGGCGCGCCAGCTCGCGGGTTAATGCGGTTTCCGGCCAGCGTTCTATCTCAGCG                           <  SRR4302537.1192049/94‑1 (MQ=60)
                                                                     GCAATAATCGGGAACACATCGCGGCTCACAAAGGCCGGATGGCGCGCCAGCTCGCGGGTTAATGCGGTTTCCGGCCAGCGTTCTATCTCAGCGG                          >  SRR4302537.4243322/1‑94 (MQ=60)
                                                                      CAATAATCGGGAACACATCGCGGCTCACAAAGGCCGGATGGCGCGCCAGCTCGCGGGTTAATGCGGTTTCCGGCCAGCGTTCTATCTCAGCGGT                         >  SRR4302537.2446609/1‑94 (MQ=60)
                                                                      CAATAATCGGGAACACATCGCGGCTCACAAAGGCCGGATGGCGCGCCAGCTCGCGGGTTAATGCGGTTTCCGGCCAGCGTTCTATCTCAGCGGT                         <  SRR4302537.2224775/94‑1 (MQ=60)
                                                                      CAATAATCGGGAACACATCGCGGCTCACAAAGGCCGGATGGCGCGCCAGCTCGCGGGTTAATGCGGTTTCCGGCCAGCGTTCTATCTCAGCGGT                         <  SRR4302537.6384371/94‑1 (MQ=60)
                                                                      CAATAATCGGGAACACATCGCGGCTCACAAAGGCCGGATGGCGCGCCAGCTCGCGGGTTAATGCGGTTTCCGGCCAGCGTTCTATCTCAGCGGT                         <  SRR4302537.4467383/94‑1 (MQ=60)
                                                                        ATAATCGGGAACACATCGCGGCTCACAAAGGCCGGATGGCGCGCCAGCTCGCGGGTTAATGCGGTTTCCGGCCAGCGTTCTATCTCAGCGGTAA                       >  SRR4302537.4523288/1‑94 (MQ=60)
                                                                        ATAATCGGGAACACATCGCGGCTCACAAAGGCCGGATGGCGCGCCAGCTCGCGGGTTAATGCGGTTTCCGGCCAGCGTTCTATCTCAGCGGTAA                       >  SRR4302537.6016786/1‑94 (MQ=60)
                                                                        ATAATCGGGAACACATCGCGGCTCACAAAGGCCGGATGGCGCGCCAGCTCGCGGGTTAATGCGGTTTCCGGCCAGCGTTCTATCTCAGCGGTAA                       >  SRR4302537.4586421/1‑94 (MQ=60)
                                                                          AATCGGGAACACATCGCGGCTCACAAAGGCCGGATGGCGCGCCAGCTCGCGGGTTAATGCGGTTTCCGGCCAGCGATCTATCTCAGCGGTAATC                     >  SRR4302537.1192528/1‑94 (MQ=60)
                                                                           ATCGGGAACACATCGCGGCTCACAAAGGCCGGATGGCGCGCCAGCTCGCGGGTTAATGCGGTTTCCGGCCAGCGTTCTATCTCAGCGGTAATCA                    <  SRR4302537.1744515/94‑1 (MQ=60)
                                                                           ATCGGGAACACATCGCGGCTCACAAAGGCCGGATGGCGCGCCAGCTCGCGGGTTAATGCGGTTTCCGGCCAGCGTTCTATCTCAGCGGTAATCA                    >  SRR4302537.5590161/1‑94 (MQ=60)
                                                                            TCGGGAACACATCGCGGCTCACAAAGGCCGGATGGCGCGCCAGCTCGCGGGTTAATGCGGTTTCCGGCCAGCGTTCTATCTCAGCGGTAATCAC                   <  SRR4302537.5173605/94‑1 (MQ=60)
                                                                            TCGGGAACACATCGCGGCTCACAAAGGCCGGATGGCGCGCCAGCTCGCGGGTTAATGCGGTTTCCGGCCAGCGTTCTATCTCAGCGGTAATCAC                   <  SRR4302537.5901261/94‑1 (MQ=60)
                                                                            TCGGGAACACATCGCGGCTCACAAAGGCCGGATGGCGCGCCAGCTCGCGGGTTAATGCGGTTTCCGGCCAGCGTTCTATCTCAGCGGTAATCAC                   <  SRR4302537.2072302/94‑1 (MQ=60)
                                                                            TCGGGAACACATCGCGGCTCACAAAGGCCGGATGGCGCGCCAGCTCGCGGGTTAATGCGGTTTCCGGCCAGCGTTCTATCTCAGCGGTAATCAC                   <  SRR4302537.1273986/94‑1 (MQ=60)
                                                                            TCGGGAACACATCGCGGCTCACAAAGGCCGGATGGCGCGCCAGCTCGCGGGTTAATGCGGTTTCCGGCCAGCGTTCTATCTCAGCGGTAATCAC                   <  SRR4302537.5173604/94‑1 (MQ=60)
                                                                             CGGGAACACATCGCGGCTCACAAAGGCCGGATGGCGCGCCAGCTCGCGGGTTAATGCGGTTTCCGGCCAGCGTTCTATCTCAGCGGTAATCACG                  >  SRR4302537.6050896/1‑94 (MQ=60)
                                                                             CGGGAACACATCGCGGCTCACAAAGGCCGGATGGCGCGCCAGCTCGCGGGTTAATGCGGTTTCCGGCCAGCGTTCTATCTCAGCGGTAATCACG                  >  SRR4302537.2382851/1‑94 (MQ=60)
                                                                              GGGAACACATCGCGGCTCACAAAGGCCGGATGGCGCGCCAGCTCGCGGGTTAATGCGGTTTCCGGCCAGCGTTCTATCTCCGCGGTAATCACGC                 >  SRR4302537.3178732/1‑94 (MQ=60)
                                                                              GGGAACACATCGCGGCTCACAAAGGCCGGATGGCGCGCCAGCTCGCGGGTTAATGCGGTTTCCGGCCAGCGTTCTATCTCAGCGGTAATCACGC                 >  SRR4302537.6074203/1‑94 (MQ=60)
                                                                               GGAACACATCGCGGCTCACAAAGGCCGGATGGCGCGCCAGCTCGCGGGTTAATGCGGTTTCCGGCCAGCGTTCTATCTCAGCGGTAATCACGCT                <  SRR4302537.2544936/94‑1 (MQ=60)
                                                                               GGAACACATCGCGGCTCACAAAGGCCGGATGGCGCGCCAGCTCGCGGGTTAATGCGGTTTCCGGCCAGCGTTCTATCTCAGCGGTAATCACGCT                >  SRR4302537.2987968/1‑94 (MQ=60)
                                                                               GGAACACATCGCGGCTCACAAAGGCCGGATGGCGCGCCAGCTCGCGGGTTAATGCGGTTTCCGGCCAGCGTTCTATCTCAGCGGTAATCACGCT                >  SRR4302537.740197/1‑94 (MQ=60)
                                                                               GGAACACATCGCGGCTCACAAAGGCCGGATGGCGCGCCAGCTCGCGGGTTAATGCGGTTTCCGGCCAGCGTTCTATCTCAGCGGTAATCACGCT                >  SRR4302537.2456072/1‑94 (MQ=60)
                                                                                 AACACATCGCGGCTCACAAAGGCCGGATGGCGCGCCAGCTCGCGGGTTAATGCGGTTTCCGGCCAGCGTTCTATCTCAGCGGTAATCACGCTTT              <  SRR4302537.4487276/94‑1 (MQ=60)
                                                                                 AACACATCGCGGCTCACAAAGGCCGGATGGCGCGCCAGCTCGCGGGTTAATGCGGTTTCCGGCCAGCGTTCTATCTCAGCGGTAATCACGCTTT              >  SRR4302537.2122360/1‑94 (MQ=60)
                                                                                 AACACATCGCGGCTCACAAAGGCCGGATGGCGCGCCAGCTCGCGGGTTAATGCGGTTTCCGGCCAGCGTTCTATCTCAGCGGTAATCACGCTTT              <  SRR4302537.4795844/94‑1 (MQ=60)
                                                                                  ACACATCGCGGCTCACAAAGGCCGGATGGCGCGCCAGCTCGCGGGTTAATGCGGTTTCCGGCCAGCGTTCTATCTCAGCGGTAATCACGCTTTG             <  SRR4302537.2250746/94‑1 (MQ=60)
                                                                                  ACACATCGCGGCTCACAAAGGCCGGATGGCGCGCCAGCTCGCGGGTTAATGCGGTTTCCGGCCAGCGTTCTATCTCAGCGGTAATCACGCTTTG             >  SRR4302537.3184924/1‑94 (MQ=60)
                                                                                  ACACATCGCGGCTCACAAAGGCCGGATGGCGCGCCAGCTCGCGGGTTAATGCGGTTTCCGGCCAGCGTTCTATCTCAGCGGTAATCACGCTTTG             <  SRR4302537.2483379/94‑1 (MQ=60)
                                                                                   CACATCGCGGCTCACAAAGGCCGGATGGCGCGCCAGCTCGCGGGTTAATGCGGTTTCCGGCCAGCGTTCTATCTCAGCGGTAATCACGCTTTGT            <  SRR4302537.693574/94‑1 (MQ=60)
                                                                                   CACATCGCGGCTCACAAAGGCCGGATGGCGCGCCAGCTCGCGGGTTAATGCGGTTTCCGGCCAGCGTTCTATCTCAGCGGTAATCACGCTTTGT            <  SRR4302537.471806/94‑1 (MQ=60)
                                                                                   CACATCGCGGCTCACAAAGGCCGGATGGCGCGCCAGCTCGCGGGTTAATGCGGTTTCCGGCCAGCGTTCTATCTCAGCGGTAATCACGCTTTGT            <  SRR4302537.2233120/94‑1 (MQ=60)
                                                                                   CACATCGCGGCTCACAAAGGCCGGATGGCGCGCCAGCTCGCGGGTTAATGCGGTTTCCGGCCAGCGTTCTATCTCAGCGGTAATCACGCTTTGT            <  SRR4302537.4753416/94‑1 (MQ=60)
                                                                                   CACATCGCGGCTCACAAAGGCCGGATGGCGCGCCAGCTCGCGGGTTAATGCGGTTTCCGGCCAGCGTTCTATCTCAGCGGTAATCACGCTTTGT            >  SRR4302537.5969975/1‑94 (MQ=60)
                                                                                   CACATCGCGGCTCACAAAGGCCGGATGGCGCGCCAGCTCGCGGGTTAATGCGGTTTCCGGCCAGCGTTCTATCTCAGCGGTAATCACGCTTTGT            <  SRR4302537.4927010/94‑1 (MQ=60)
                                                                                   CACATCGCGGCTCACAAAGGCCGGATGGCGCGCCAGCTCGCGGGTTAATGCGGTTTCCGGCCAGCGTTCTATCTCAGCGGTAATCACGCTTTGT            >  SRR4302537.3518452/1‑94 (MQ=60)
                                                                                     CATCGCGGCTCACAAAGGCCGGATGGCGCGCCAGCTCGCGGGTTAATGCGGTTTCCGGCCAGCGTTCTATCTCAGCGGTAATCACGCTTTGTTG          >  SRR4302537.5086570/1‑94 (MQ=60)
                                                                                      ATCGCGGCTCACAAAGGCCGGATGGCGCGCCAGCTCGCGGGTTAATGCGGTTTCCGGCCAGCGTTCTATCTCAGCGGTAATCACGCTTTGTTGA         >  SRR4302537.2589117/1‑94 (MQ=60)
                                                                                      ATCGCGGCTCACAAAGGCCGGATGGCGCGCCAGCTCGCGGGTTAATGCGGTTTCCGGCCAGCGTTCTATCTCAGCGGTAATCACGCTTTGTTGA         >  SRR4302537.1859640/1‑94 (MQ=60)
                                                                                        CGCGGCTCACAAAGGCCGGATGGCGCGCCAGCTCGCGGGTTAATGCGGTTTCCGGCCAGCGTTCTATCTCAGCGGTAATCACGCTTTGTTGAAA       >  SRR4302537.1411491/1‑94 (MQ=60)
                                                                                          CGGCTCACAAAGGCCGGATGGCGCGCCAGCTCGCGGGTTAATGCGGTTTCCGGCCGGCGTTCTATCTCAGCGGTAATCACGCTTTGTTGAAAAG     >  SRR4302537.183615/1‑94 (MQ=60)
                                                                                          CGGCTCACAAAGGCCGGATGGCGCGCCAGCTCGCGGGTTAATGCGGTTTCCGGCCAGCGTTCTATCTCAGCGGTAATCACGCTTTGTTGAAAAG     <  SRR4302537.5547150/94‑1 (MQ=60)
                                                                                          CGGCTCACAAAGGCCGGATGGCGCGCCAGCTCGCGGGTTAATGCGGTTTCCGGCCAGCGTTCTATCTCAGCGGTAATCACGCTTTGTTGAAAAG     >  SRR4302537.3211289/1‑94 (MQ=60)
                                                                                           GGCTCACAAAGGCCGGATGGCGCGCCAGCTCGCGGGTTAATGCGGTTTCCGGCCAGCGTTCTATCTCAGCGGTAATCACGCTTTGTTGAAAAGG    >  SRR4302537.5702734/1‑94 (MQ=60)
                                                                                           GGCTCACAAAGGCCGGATGGCGCGCCAGCTCGCGGGTTAATGCGGTTTCCGGCCAGCGTTCTATCTCAGCGGTAATCACGCTTTGTTGAAAAGG    <  SRR4302537.5023605/94‑1 (MQ=60)
                                                                                            GCTCACAAAGGCCGGATGGCGCGCCAGCTCGCGGGTTAGTGCGGTTTCCGGACCGCGTTCTATCTCAGCGGTAATCACGCTTTGTTGAAAAGGC   <  SRR4302537.6383457/94‑1 (MQ=60)
                                                                                            GCTCACAAAGGCCGGATGGCGCGCCAGCTCGCGGGTTAATGCGGTTTCCGGCCAGCGTTCTATCTCAGCGGTAATCACGCTTTGTTGAAAAGGC   <  SRR4302537.2191812/94‑1 (MQ=60)
                                                                                             CTCACAAAGGCCGGATGGCGCGCCAGCTCGCGGGTTAATGCGGTTTCCGGCCAGCGTTCTATCTCAGCGGTAATCACGCTTTGTTGAAAAGGCA  <  SRR4302537.5744418/94‑1 (MQ=60)
                                                                                             CTCACAAAGGCCGCATGGCGCGCCAGCTCGCGGGTTAATGCGGTTGCCGGCAAGCGTACTATCTCAGCGGTAATCACGCTTTGTTGAAAAGGCA  <  SRR4302537.95699/94‑1 (MQ=60)
                                                                                             |                                                                                             
GCAAGTAACTGCCACGGTGCAGTCGGCAGGTGGAGCTTATCGAAAAGCTGCTTCTGAGTCAGACGGTCAGCAATAATCGGGAACACATCGCGGTTCACAAAGGCCGGATGGCGCGCCAGCTCGCGGGTTAATGCGGTTTCCGGCCAGCGTTCTATCTCAGCGGTAATCACGCTTTGTTGAAAAGGCA  >  NC_000913/551516‑551702

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 2 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑