Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I2 R1 882 254.4 12957588 98.0% 12698436 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 654,888 C→T V102M (GTG→ATG)  dcuC ← DcuC

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913654,8880CT95.8% 744.1 / 10.2 215V102M (GTG→ATG) dcuCDcuC
Reads supporting (aligned to +/- strand):  ref base C (7/2);  new base T (96/110);  total (103/112)
Fisher's exact test for biased strand distribution p-value = 9.06e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.65e-01

Only 100 of 230 total aligned reads displayed.

GAGACATCAGACAGGCGACAAAATAGGCGGCAATCATCAGCAGGTAAGGGGAGTTAATATACTGCAATGGTTTTGACGCCAGCTTGACCACCATATCATTCGCGCCGATATGGGTCATGTAAGCGGCAAATCCACACAGCATCATAATCATCATGCCGAGGTCGCCGCCGCGGCTCATTA  >  NC_000913/654798‑654977
                                                                                          |                                                                                         
gagaCATCAGACAGGCGACAAAATAGGCGGCAATCATCAGCAGGTAAGGGGAGTTAATATACTGCAATGGTTTTGACGCCAGCTTGAccatca                                                                                         >  1:4522224/1‑93 (MQ=255)
gagaCATCAGACAGGCGACAAAATAGGCGGCAATCATCAGCAGGTAAGGGGAGTTAATATACTGCAATGGTTTTGACGCCAGCTTGAccatca                                                                                         <  1:5655050/93‑1 (MQ=255)
 agaCATCAGACAGGCGACAAAATAGGCGGCAATCATCAGCAGGTAAGGGGAGTTAATATACTGCAATGGTTTTGACGCCAGCTTGACcatcat                                                                                        <  2:2981611/93‑1 (MQ=255)
 agaCATCAGACAGGCGACAAAATAGGCGGCAATCATCAGCAGGTAAGGGGAGTTAATATACCGCAATGGTTTTGACGCCAGCTTGACcatcat                                                                                        >  1:1751350/1‑93 (MQ=255)
  gaCATCAGACAGGCGACAAAATAGGCGGCAATCATCAGCAGGTAAGGGGAGTTAATATACTGCAATGGTTTTGACGCCAGCTTGACCATCata                                                                                       >  2:3106300/1‑93 (MQ=255)
   aCATCAGACAGGCGACAAAATAGGCGGCAATCATCAGCAGGTAAGGGGAGTTAATATACTGCAATGGTTTTGACGCCAGCTTGACCATCatat                                                                                      <  2:527509/93‑1 (MQ=255)
    cATCAGACAGGCGACAAAATAGGCGGCAATCATCAGCAGGTAAGGGGAGTTAATATACTGCAATGGTTTTGACGCCAGCTTGACCATCATATc                                                                                     <  2:4147033/93‑1 (MQ=255)
     aTCAGACAGGCGACAAAATAGGCGGCAATCATCAGCAGGTAAGGGGAGTTAATATACTGCAATGGTTTTGACGCCAGCTTGACCATCATATCa                                                                                    <  2:4158456/93‑1 (MQ=255)
      tCAGACAGGCGACAAAATAGGCGGCAATCATCAGCAGGTAAGGGGAGTTAATATACTGCAATGGTTTTGACGCCAGCTTGACCatcatatcat                                                                                   >  1:1640705/1‑93 (MQ=255)
      tCAGACAGGCGACAAAATAGGCGGCAATCATCAGCAGGTAAGGGGAGTTAATATACTGCAATGGTTTTGACGCCAGCTTGACCACCATATCAt                                                                                   >  2:1141148/1‑93 (MQ=255)
         gACAGGCGACAAAATAGGCGGCAATCATCAGCAGGTAAGGGGAGTTAATATACTGCAATGGTTTTGACGCCAGCTTGACCATCATATCATTcg                                                                                >  1:4471707/1‑93 (MQ=255)
          aCAGGCGACAAAATAGGCGGCAATCATCAGCAGGTAAGGGGAGTTAATATACTGCAATGGTTTTGACGCCAGCTTGACCATCATATCATTcgc                                                                               <  1:441873/93‑1 (MQ=255)
          aCAGGCGACAAAATAGGCGGCAATCATCAGCAGGTAAGGGGAGGTAATATACTGCAATGGTTTTGACGCCAGCTTGACCATCATATCATTcgc                                                                               >  2:5082998/1‑93 (MQ=255)
            aGGCGACAAAATAGGCGGCAATCATCAGCAGGTAAGGGGAGTTAATATACTGCAATGGTTTTGACGCCAGCTTGACCATCATATCATTCgcgc                                                                             >  2:5134951/1‑93 (MQ=255)
            aGGCGACAAAATAGGCGGCAATCATCAGCAGGTAAGGGGAGTTAATATACTGCAATGGTTTTGACGCCAGCTTGACCATCATATCATTCgcgc                                                                             <  2:1371461/93‑1 (MQ=255)
                 aCAAAATAGGCGGCAATCATCAGCAGGTAAGGGGAGTTAATATACTGCAATGGTTTTGACGCCAGCTTGACCATCATATCATTCGCGCCGata                                                                        <  2:2681148/93‑1 (MQ=255)
                 aCAAAATAGGCGGCAATCATCAGCAGGTAAGGGGAGTTAATATACTGCAATGGTTTTGACGCCAGCTTGACCATCATATCATTCGCGCCGata                                                                        >  2:2753890/1‑93 (MQ=255)
                 aCAAAATAGGCGGCAATCATCAGCAGGTAAGGGGAGTTAATATACTGCAATGGTTTTGACGCCAGCTTGACCATCATATCATTCGCGCCGata                                                                        <  2:5556955/93‑1 (MQ=255)
                   aaaaTAGGCGGCAATCATCAGCAGGTAAGGGGAGTTAATATACTGCAATGGTTTTGACGCCAGCTTGACCATCATATCATTCGCGCCGATATg                                                                      <  2:210666/93‑1 (MQ=255)
                   aaaaTAGGCGGCAATCATCAGCAGGTAAGGGGAGTTAATATACTGCAATGGTTTTGACGCCAGCTTGACCACCATATCATTCGCGCCGATATg                                                                      >  2:3809508/1‑93 (MQ=255)
                    aaaTAGGCGGCAATCATCAGCAGGTAAGGGGAGTTAATATACTGCAATGGTTTTGACGCCAGCTTGACCATCATATCATTCGCGCCGATATgg                                                                     >  1:2669433/1‑93 (MQ=255)
                     aaTAGGCGGCAATCATCAGCAGGTAAGGGGAGTTAATATACTGCAATGGTTTTGACGCCAGCTTGACCATCATATCATTCGCGCCGATATggg                                                                    <  2:6048405/93‑1 (MQ=255)
                      aTAGGCGGCAATCATCAGCAGGTAAGGGGAGTTAATATACTGCAATGGTTTTGACGCCAGCTTGACCATCATATCATTCGCGCCGATATGGGt                                                                   <  1:344419/93‑1 (MQ=255)
                      aTAGGCGGCAATCATCAGCAGGTAAGGGGAGTTAATATACTGCAATGGTTTTGACGCCAGCTTGACCATCATATCATTCGCGCCGATATGGGt                                                                   >  1:4097690/1‑93 (MQ=255)
                         ggcggcAATCATCAGCAGGTAAGGGGAGTTAATATACTGCAATGGTTTTGACGCCAGCTTGACCATCATATCATTCGCGCCGATATGGGTCAt                                                                <  2:538279/93‑1 (MQ=255)
                         ggcggcAATCATCAGCAGGTAAGGGGAGTTAATATACTGCAATGGTTTTGACGCCAGCTTGACCATCATATCATTCGCGCCGATATGGGTCAt                                                                >  1:2012357/1‑93 (MQ=255)
                           cggcAATCATCAGCAGGTAAGGGGAGTTAATATACTGCAATGGTTTTGACGCCAGCTTGACCATCATATCATTCGCGCCGATATGGGTCATGt                                                              <  1:1412415/93‑1 (MQ=255)
                             gcAATCATCAGCAGGTAAGGGGAGTTAATATACTGCAATGGTTTTGACGCCAGCTTGACCATCATATCATTCGCGCCGATATGGGTCATGTaa                                                            >  2:4307079/1‑93 (MQ=255)
                              cAATCATCAGCAGGTAAGGGGAGTTAATATACTGCAATGGTTTTGACGCCAGCTTGACCATCATATCATTCGCGCCGATATGGGTCATGTAAg                                                           <  1:830161/93‑1 (MQ=255)
                               aaTCATCAGCAGGTAAGGGGAGTTAATATACTGCAATGGTTTTGACGCCAGCTTGACCATCATATCATTCGCGCCGATATGGGTCATGTAAGc                                                          <  1:1235944/93‑1 (MQ=255)
                               aaTCATCAGCAGGTAAGGGGAGTTAATATACTGCAATGGTTTTGACGCCAGCTTGACCATCATATCATTCGCGCCGATATGGGTCATGTAAGc                                                          <  2:3947501/93‑1 (MQ=255)
                               aaTCATCAGCAGGTAAGGGGAGTTAATATACTGCAATGGTTTTGACGCCAGCTTGACCATCATATCATTCGCGCCGATATGGGTCATGTAAGc                                                          >  1:261212/1‑93 (MQ=255)
                                atcatcAGCAGGTAAGGGGAGTTAATATACTGCAATGGTTTTGACGCCAGCTTGACCATCATATCATTCGCGCCGATATGGGTCATGTAAGCg                                                         >  1:3742109/1‑93 (MQ=255)
                                atcatcAGCAGGTAAGGGGAGTTAATATACTGCAATGGTTTTGACGCCAGCTTGACCACCATATCATTCGCGCCGATATGGGTCATGTAAGCg                                                         <  1:285012/93‑1 (MQ=255)
                                atcaacAGCAGGTAAGGGGAGTTAATATACTGCAATGGTTTTGACGCCAGCTTGACCATCATATCATTCGCGCCGATATGGGTCATGTAAGCg                                                         <  2:6476047/93‑1 (MQ=255)
                                 tcatcaGCAGGTAAGGGGAGTTAATATACTGCAATGGTTTTGACGCCAGCTTGACCATCATATCATTCGCGCCGATATGGGTCATGTAAGCgg                                                        >  1:5060266/1‑93 (MQ=255)
                                 tcatcaGCAGGTAAGGGGAGTTAATATACTGCAATGGTTTTGACGCCAGCTTGACCATCATATCATTCGCGCCGATATGGGTCATGTAAGCgg                                                        >  1:2450879/1‑93 (MQ=255)
                                 tcatcaGCAGGTAAGGGGAGTTAATATACTGCAATGGTTTTGACGCCAGCTTGACCATCATATCATTCGCGCCGATATGGGTCATGTAAGCgg                                                        <  1:866657/93‑1 (MQ=255)
                                  catcaGCAGGTAAGGGGAGTTAATATACTGCAATGGTTTTGACGCCAGCTTGACCATCATATCATTCGCGCCGATATGGGTCATGTAAGCGGc                                                       <  1:4918384/93‑1 (MQ=255)
                                    tcaGCAGGTAAGGGGAGTTAATATACTGCAATGGTTTTGACGCCAGCTTGACCATCATATCATTCGCGCCGATATGGGTCATGTAAGCGGCaa                                                     >  2:2507039/1‑93 (MQ=255)
                                      agcagGTAAGGGGAGTTAATATACTGCAATGGTTTTGACGCCAGCTTGACCATCATATCATTCGCGCCGATATGGGTCATGTAAGCGGCAAAt                                                   <  2:3599203/93‑1 (MQ=255)
                                      agcagGTAAGGGGAGTTAATATACTGCAATGGTTTTGACGCCAGCTTGACCATCATATCATTCGCGCCGATATGGGTCATGTAAGCGGCAAAt                                                   >  2:928098/1‑93 (MQ=255)
                                      agcagGTAAGGGGAGTTAATATACTGCAATGGTTTTGACGCCAGCTTGACCATCATATCATTCGCGCCGATATGGGTCATGTAAGCGGCAAAt                                                   <  1:3332938/93‑1 (MQ=255)
                                       gcagGTAAGGGGAGTTAATATACTGCAATGGTTTTGACGCCAGCTTGACCATCATATCATTCGCGCCGATATGGGTCATGTAAGCGGCAAATc                                                  >  2:2208342/1‑93 (MQ=255)
                                          ggTAAGGGGAGTTAATATACTGCAATGGTTTTGACGCCAGCTTGACCATCATATCATTCGCGCCGATATGGGTCATGTAAGCGGCAAATCcac                                               <  2:1020160/93‑1 (MQ=255)
                                          ggTAAGGGGAGTTAATATACTGCAATGGTTTTGACGCCAGCTTGACCATCATATCATTCGCGCCGATATGGGTCATGTAAGCGGCAAATCcac                                               >  2:1440237/1‑93 (MQ=255)
                                           gTAAGGGGAGTTAATATACTGCAATGGTTTTGACGCCAGCTTGACCATCATATCATTCGCGCCGATATGGGTCATGTAAGCGGCAAATCcaca                                              >  1:4349959/1‑93 (MQ=255)
                                            tAAGGGGAGTTAATATACTGCAATGGTTTTGACGCCAGCTTGACCATCATATCATTCGCGCCGATATGGGTCATGTAAGCGGCAAATCcacac                                             <  2:518905/93‑1 (MQ=255)
                                            tAAGGGGAGTTAATATACTGCAATGGTTTTGACGCCAGCTTGACCATCATATCATTCGCGCCGATATGGGTCATGTAAGCGGCAAATCcacac                                             >  2:3131166/1‑93 (MQ=255)
                                             aaGGGGAGTTAATATACTGCAATGGTTTTGACGCCAGCTTGACCATCATATCATTCGCGCCGATATGGGTCATGTAAGCGGCAAATCcacaca                                            <  2:6288873/93‑1 (MQ=255)
                                             aaGGGGAGTTAATATACTGCAATGGTTTTGACGCCAGCTTGACCATCATATCATTCGCGCCGATATGGGTCATGTAAGCGGCAAATCcacaca                                            >  2:5980921/1‑93 (MQ=255)
                                              aGGGGAGTTAATATACTGCAATGGTTTTGACGCCAGCTTGACCATCATATCATTCGCGCCGATATGGGTCATGTAAGCGGCAAATCCACACAg                                           >  2:4437363/1‑93 (MQ=255)
                                              aGGGGAGTTAATATACTGCAATGGTTTTGACGCCAGCTTGACCATCATATCATTCGCGCCGATATGGGTCATGTAAGCGGCAAATCCACACAg                                           <  1:3813070/93‑1 (MQ=255)
                                               ggggAGTTAATATACTGCAATGGTTTTGACGCCAGCTTGACCATCATATCATTCGCGCCGATATGGGTCATGTAAGCGGCAAATCCACACAGc                                          >  1:5585455/1‑93 (MQ=255)
                                               ggggAGTTAATATACTGCAATGGTTTTGACGCCAGCTTGACCATCATATCATTCGCGCCGATATGGGTCATGTAAGCGGCAAATCCACACAGc                                          >  1:6314534/1‑93 (MQ=255)
                                                gggAGTTAATATACTGCAATGGTTTTGACGCCAGCTTGACCATCATATCATTCGCGCCGATATGGGTCATGTAAGCGGCAAATCCACACAGca                                         <  1:2199862/93‑1 (MQ=255)
                                                 ggAGTTAATATACTGCAATGGTTTTGACGCCAGCTTGACCATCATATCATTCGCGCCGATATGGGTCATGTAAGCGGCAAATCCACACAGcat                                        >  2:6137625/1‑93 (MQ=255)
                                                  gAGTTAATATACTGCAATGGTTTTGACGCCAGCTTGACCATCATATCATTCGCGCCGATATGGGTCATGTAAGCGGCAAATCCACACAGcatc                                       >  1:5449312/1‑93 (MQ=255)
                                                  gAGTTAATATACTGCAATGGTTTTGACGCCAGCTTGACCATCATATCATTCGCGCCGATATGGGTCATGTAAGCGGCAAATCCACACAGcata                                       >  1:4661962/1‑92 (MQ=255)
                                                   aGTTAATATACTGCAATGGTTTTGACGCCAGCTTGACCATCATATCATTCGCGCCGATATGGGTCATGTAAGCGGCAAATCCACACAGcatca                                      <  1:6234976/93‑1 (MQ=255)
                                                   aGTTAATATACTGCAATGGTTTTGACGCCAGCTTGACCATCATATCATTCGCGCCGATATGGGTCATGTAAGCGGCAAATCCACACAGcatca                                      <  1:2286012/93‑1 (MQ=255)
                                                   aGTTAATATACTGCAATGGTTTTGACGCCAGCTTGACCATCATATCATTCGCGCCGATATGGGTCATGTAAGCGGCAAATCCACACAGcatca                                      <  2:160128/93‑1 (MQ=255)
                                                     ttAATATACTGCAATGGTTTTGACGCCAGCTTGACCATCATATCATTCGCGCCGATATGGGTCATGTAAGCGGCAAATCCACACAGCATCATa                                    <  2:3654282/93‑1 (MQ=255)
                                                      tAATATACTGCAATGGTTTTGACGCCAGCTTGACCATCATATCATTCGCGCCGATATGGGTCATGTAAGCGGCAAATCCACACAGCATCATaa                                   >  2:4223056/1‑93 (MQ=255)
                                                       aaTATACTGCAATGGTTTTGACGCCAGCTTGACCATCATATCATTCGCGCCGATATGGGTCATGTAAGCGGCAAATCCACACAGCATCATAat                                  >  2:6031680/1‑93 (MQ=255)
                                                        atatACTGCAATGGTTTTGACGCCAGCTTGACCATCATATCATTCGCGCCGATATGGGTCATGTAAGCGGCAAATCCACACAGCATCATAatc                                 >  2:1741761/1‑93 (MQ=255)
                                                         tataCTGCAATGGTTTTGACGCCAGCTTGACCATCATATCATTCGCGCCGATATGGGTCATGTAAGCGGCAAATCCACACAGCATCATAatca                                >  2:5766582/1‑93 (MQ=255)
                                                         tataCTGCAATGGTTTTGACGCCAGCTTGACCATCATATCATTCGCGCCGATATGGGTCATGTAAGCGGCAAATCCACACAGCATCATAatca                                >  2:453314/1‑93 (MQ=255)
                                                         tataCTGCAATGGTTTTGACGCCAGCTTGACCATCATATCATTCGCGCCGATATGGGTCATGTAAGCGGCAAATCCACACAGCATCATAatca                                >  2:1282595/1‑93 (MQ=255)
                                                         tataCTGCAATGGTTTTGACGCCAGCTTGACCATCATATCATTCGCGCCGATATGGGTCATGTAAGCGGCAAATCCACACAGCATCATAatca                                >  1:3649135/1‑93 (MQ=255)
                                                         tataCTGCAATGGTTTTGACGCCAGCTTGACCACCATATCATTCGCGCCGATATGGGTCATGTAAGCGGCAAATCCACACAGCATCATAatca                                >  2:84011/1‑93 (MQ=255)
                                                           taCTGCAATGGTTTTGACGCCAGCTTGACCATCATATCATTCGCGCCGATATGGGTCATGTAAGCGGCAAATCCACACAGCATCATAatcatc                              >  2:4922087/1‑93 (MQ=255)
                                                           taCTGCAATGGTTTTGACGCCAGCTTGACCATCATATCATTCGCGCCGATATGGGTCATGTAAGCGGCAAATCCACACAGCATCATAatcatc                              <  2:4033009/93‑1 (MQ=255)
                                                           taCTGCAATCGTTGTGACGCCCGCTTGACCTCCATATCATTCGCGCCGATATGGGTCCTGTGAGCGGCAAATCCACAGAGCATCATAatcttc                              <  2:354691/93‑1 (MQ=255)
                                                            aCTGCAATGGTTTTGACGCCAGCTTGACCATCATATCATTCGCGCCGATATGGGTCATGTAAGCGGCAAATCCACACAGCATCATAAtcatca                             <  2:3663195/93‑1 (MQ=255)
                                                              tGCAATGGTTTTGACGCCAGCTTGACCATCATATCATTCTCGCCGATATGGGTCATGTAAGCGGCAAATCCACACAGCATCATAATCATCATg                           >  1:2479439/1‑93 (MQ=255)
                                                               gCAATGGTTTTGACGCCAGCTTGACCATCATATCATTCGCGCCGATATGGGTCATGTAAGCGGCAAATCCACACAGCATCATAATCATCATGc                          >  2:1172815/1‑93 (MQ=255)
                                                                cAATGGTTTTGACGCCAGCTTGACCATCATATCATTCGCGCCGATATGGGTCATGTAAGCGGCAAATCCACACAGCATCATAATCATCATGcc                         <  2:3318242/93‑1 (MQ=255)
                                                                 aaTGGTTTTGACGCCAGCTTGACCATCATATCATTCGCGCCGATATGGGTCATGTAAGCGGCAAATCCACACAGCATCATAATCATCATGCCg                        >  1:6073463/1‑93 (MQ=255)
                                                                  aTGGTTTTGACGCCAGCTTGACCATCATATCATTCGCGCCGATATGGGTCATGTAAGCGGCAAATCCACACAGCATCATAATCATCATGCCGa                       <  1:3549280/93‑1 (MQ=255)
                                                                   tGGTTTTGACGCCAGCTTGACCATCATATCATTCGCGCCGATATGGGTCATGTAAGCGGCAAATCCACACAGCATCATAATCATCATGCCGAg                      <  2:5914634/93‑1 (MQ=255)
                                                                    ggTTTTGACGCCAGCTTGACCATCATATCATTCGCGCCGATATGGGTCATGTAAGCGGCAAATCCACACAGCATCATAATCATCATGCCGAgg                     <  1:510577/93‑1 (MQ=255)
                                                                     gTTTTGACGCCAGCTTGACCATCATATCATTCGCGCCGATATGGGTCATGTAAGCGGCAAATCCACACAGCATCATAATCATCATGCCGAGGt                    <  2:4739785/93‑1 (MQ=255)
                                                                      ttttGACGCCAGCTTGACCATCATATCATTCGCGCCGATATGGGTCATGTAAGCGGCAAATCCACACAGCATCATAATCATCATGCCGAGGTc                   <  1:1894479/93‑1 (MQ=255)
                                                                      ttttGACGCCAGCTTGACCATCATATCATTCGCGCCGATATGGGTCATGTAAGCGGCAAATCCACACAGCATCATAATCATCATGCCGAGGTc                   <  1:5335211/93‑1 (MQ=255)
                                                                        ttGACGCCAGCTTGACCATCATATCATTCGCGCCGATATGGGTCATGTAAGCGGCAAATCCACACAGCATCATAATCATCATGCCGAGGTcgc                 <  1:4297007/93‑1 (MQ=255)
                                                                        ttGACGCCAGCTTGACCATCATATCATTCGCGCCGATATGGGTCATGTAAGCGGCAAATCCACACAGCATCATAATCATCATGCCGAGGTcgc                 >  1:5716191/1‑93 (MQ=255)
                                                                        ttGACGCCAGCTTGACCATCATATCATTCGCGCCGATATGGGTCATGTAAGCGGCAAATCCACACAGCATCATAATCATCATGCCGAGGTcgc                 >  2:1687403/1‑93 (MQ=255)
                                                                        ttGACGCCAGCTTGACCATCATATCATTCGCGCCGATATGGGTCATGTAAGCGGCAAATCCACACAGCATCATAATCATCATGCCGAGGTcgc                 <  2:2281918/93‑1 (MQ=255)
                                                                         tGACGCCAGCTTGACCATCATATCATTCGCGCCGATATGGGTCATGTAAGCGGCAAATCCACACAGCATCATAATCATCATGCCGAGGTcgcc                <  1:1092927/93‑1 (MQ=255)
                                                                           aCGCCAGCTTGACCATCATATCATTCGCGCCGATATGGGTCATGTAAGCGGCAAATCCACACAGCATCATAATCATCATGCCGAGGTcgccgc              <  2:1964420/93‑1 (MQ=255)
                                                                           aCGCCAGCTTGACCATCATATCATTCGCGCCGATATGGGTCATGTAAGCGGCAAATCCACACAGCATCATAATCATCATGCCGAGGTcgccgc              <  2:3401941/93‑1 (MQ=255)
                                                                             gCCAGCTTGACCATCATATCATTCGCGCCGATATGGGTCATGTAAGCGGCAAATCCACACAGCATCATAATCATCATGCCGAGGTcgccgccg            >  2:5246603/1‑93 (MQ=255)
                                                                              ccAGCTTGACCATCATATCATTCGCGCCGATATGGGTCATGTAAGCGGCAAATCCACACAGCATCATAATCATCATGCCGAGGTcgccgccgc           <  1:4986816/93‑1 (MQ=255)
                                                                               cAGCTTGACCATCATATCATTCGCGCCGATATGGGTCATGTAAGCGGCAAATCCACACAGCATCATAATCATCATGCCGAGGTCGCCGCcgcg          >  1:2919418/1‑93 (MQ=255)
                                                                                   ttGACCATCATATCATTCGCGCCGATATGGGTCATGTAAGCGGCAAATCCACACAGCATCATAATCATCATGCCGAGGTCGCCGCCGCGGCTc      >  1:133552/1‑93 (MQ=255)
                                                                                    tGACCATCATATCATTCGCGCCGATATGGGTCATGTAAGCGGCAAATCCACACAGCATCATAATCATCATGCCGAGGTCGCCGCCGCGGCTCa     <  2:6417214/93‑1 (MQ=255)
                                                                                     gACCATCATATCATTCGCGCCGATATGGGTCATGTAAGCGGCAAATCCACACAGCATCATAATCATCATGCCGAGGTCGCCGCCGCGGCTCAt    <  2:642107/93‑1 (MQ=255)
                                                                                     gACCATCATATCATTCGCGCCGATATGGGTCATGTAAGCGGCAAATCCACACAGCATCATAATCATCATGCCGAGGTCGCCGCCGCGGCTCAt    >  2:4494221/1‑93 (MQ=255)
                                                                                       ccatcaTATCATTCGCGCCGATATGGGTCATGTAAGCGGCAAATCCACACAGCATCATAATCATCATGCCGAGGTCGCCGCCGCGGCTCATTa  <  1:289875/93‑1 (MQ=255)
                                                                                          |                                                                                         
GAGACATCAGACAGGCGACAAAATAGGCGGCAATCATCAGCAGGTAAGGGGAGTTAATATACTGCAATGGTTTTGACGCCAGCTTGACCACCATATCATTCGCGCCGATATGGGTCATGTAAGCGGCAAATCCACACAGCATCATAATCATCATGCCGAGGTCGCCGCCGCGGCTCATTA  >  NC_000913/654798‑654977

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
CAGAGACATCAGACAGGCGACAAAATAGGCGGCAATCATCAGCAGGTAAGGGGAGTTAATATACTGCAATGGTTTTGACGCCAGCTTGACCACCATATCATTCGCGCCGATATGGGTCATGTAAGCGGCAAATCCACACAGCATCATAATCATCATGCCGAGGTCGCCGCCGCGGCTCATTAGTA  >  NC_000913/654796‑654980
                                                                                            |                                                                                            
CAGAGACATCAGACAGGCGACAAAATAGGCGGCAATCATCAGCAGGTATGGGGAGTTAATATACTGCAATGGTTTTGACGCCAGCTTGACCATC                                                                                             >  SRR4302537.4801101/1‑94 (MQ=60)
CAGAGACATCAGACAGGCGACAAAATAGGCGGCAATCATCAGCAGGTAAGGGGAGTTAATATACTGCAATGGTTTTGACGCCAGCTTGACCATC                                                                                             >  SRR4302537.3950668/1‑94 (MQ=60)
CAGAGACATCAGACAGGCGACAAAATAGGCGGCAATCATCAGCAGGTAAGGGGAGTTAATATACTGCAATGGTTTTGACGCCAGCTTGACCATC                                                                                             >  SRR4302537.721100/1‑94 (MQ=60)
 AGAGACATCAGACAGGCGACAAAATAGGCGGCAATCATCAGCAGGTAAGGGGAGTTAATATACTGCAATGGTTTTGACGCCAGCTTGACCATCA                                                                                            >  SRR4302537.4553508/1‑94 (MQ=60)
  GAGACATCAGACAGGCGACAAAATAGGCGGCAATCATCAGCAGGTAAGGGGAGTTAATATACTGCAATGGTTTTGACGCCAGCTTGACCATCAT                                                                                           <  SRR4302537.5693747/94‑1 (MQ=60)
  GAGACATCAGACAGGCGACAAAATAGGCGGCAATCATCAGCAGGTAAGGGGAGTTAATATACCGCAATGGTTTTGACGCCAGCTTGACCATCAT                                                                                           >  SRR4302537.1763557/1‑94 (MQ=60)
    GACATCAGACAGGCGACAAAATAGGCGGCAATCATCAGCAGGTAAGGGGAGTTAATATACTGCAATGGTTTTGACGCCAGCTTGACCATCATAT                                                                                         <  SRR4302537.1567040/94‑1 (MQ=60)
      CATCAGACAGGCGACAAAATAGGCGGCAATCATCAGCAGGTAAGGGGAGCTAATATACTGCAAAGGTTTTGACGCCAGCTTGACCATCATATCA                                                                                       >  SRR4302537.363265/1‑94 (MQ=60)
       ATCAGACAGGCGACAAAATAGGCGGCAATCATCAGCAGGTAAGGGGAGTTAATATACTGCAATGGTTTTGACGCCAGCTTGACCATCATATCAT                                                                                      >  SRR4302537.1652199/1‑94 (MQ=60)
         CAGACAGGCGACAAAATAGGCGGCAATCATCAGCAGGTAAGGGGAGTTAATATACTGCAATGGTTTTGACGCCAGCTTGACCATCATATCATTC                                                                                    <  SRR4302537.2884104/94‑1 (MQ=60)
         CAGACAGGCGACAAAATAGGCGGCAATCATCAGCAGGTAAGGGGAGTTAATATACTGCAATGGTTTTGACGCCAGCTTGACCACCATATCATTC                                                                                    >  SRR4302537.4271510/1‑94 (MQ=60)
          AGACAGGCGACAAAATAGGCGGCAATCATCAGCAGGTAAGGGGAGTTAATATACTGCAATGGTTTTGACGCCAGCTTGACCATCATATCATTCG                                                                                   >  SRR4302537.4502640/1‑94 (MQ=60)
          AGACAGGCGACAAAATAGGCGGCAATCATCAGCAGGTAAGGGGAGTTAATATACTGCAATGGTTTTGACGCCAGCTTGACCATCATATCATTCG                                                                                   >  SRR4302537.1809424/1‑94 (MQ=60)
           GACAGGCGACAAAATAGGCGGCAATCATCAGCAGGTAAGGGGAGTTAATATACTGCAATGGTTTTGACGCCAGCTTGACCATCATATCATTCGC                                                                                  >  SRR4302537.2988188/1‑94 (MQ=60)
           GACAGGCGACAAAATAGGCGGCAATCATCAGCAGGTAAGGGGAGTTAATATACTGCAATGGTTTTGACGCCAGCTTGACCATCATATCATTCGC                                                                                  <  SRR4302537.4438903/94‑1 (MQ=60)
            ACAGGCGACAAAATAGGCGGCAATCATCAGCAGGTAAGGGGAGTTAATATACTGCAATGGTTTTGACGCCAGCTTGACCATCATATCATTCGCG                                                                                 <  SRR4302537.6204667/94‑1 (MQ=60)
            ACAGGCGACAAAATAGGCGGCAATCATCAGCAGGTAAGGGGAGTTAATATACTGCAATGGTTTTGACGCCAGCTTGACCATCATATCATTCGCG                                                                                 <  SRR4302537.5255967/94‑1 (MQ=60)
            ACAGGCGACAAAATAGGCGGCAATCATCAGCAGGTAAGGGGAGTTAATATACTGCAATGGTTTTGACGCCAGCTTGACCATCATATCATTCGCG                                                                                 >  SRR4302537.5742742/1‑94 (MQ=60)
            ACAGGCGACAAAATAGGCGGCAATCATCAGCAGGTAAGGGGAGTTAATATACTGCAATGGTTTTGACGCCAGCTTGACCATCATATCATTCGCG                                                                                 <  SRR4302537.444941/94‑1 (MQ=60)
             CAGGCGACAAAATAGGCGGCAATCATCAGCAGGTAAGGGGAGTTAATATACTGCAATGGTTTTGACGCCAGCTTGACCATCATATCATTCGCGC                                                                                <  SRR4302537.192490/94‑1 (MQ=60)
              AGGCGACAAAATAGGCGGCAATCATCAGCAGGTAAGGGGAGTTAATATACTGCAATGGTTTTGACGCCAGCTTGACCATCATATCATTCGCGCC                                                                               <  SRR4302537.658362/94‑1 (MQ=60)
                 CGACAAAATAGGCGGCAATCATCAGCAGGTAAGGGGATTTAATATACTGCAATGGTTTTGACGCCAGCTTGACCATCATATCATTCGCGCCGAT                                                                            <  SRR4302537.1368351/94‑1 (MQ=60)
                 CGACAAAATAGGCGGCAATCATCAGCAGGTAAGGGGAGTTAATATACTGCAATGGTTTTGACGCCAGCTTGACCATCATATCATTCGCGCCGAT                                                                            <  SRR4302537.4481507/94‑1 (MQ=60)
                 CGACAAAATAGGCGGCAATCATCAGCAGGTAAGGGGAGTTAATATACTGCAATGGTTTTGACGCCAGCTTGACCATCATATCATTCGCGCCGAT                                                                            <  SRR4302537.162090/94‑1 (MQ=60)
                  GACAAAATAGGCGGCAATCATCAGCAGGTAAGGGGAGTTAATATACTGCAATGGTTTTGACGCCAGCTTGACCATCATATCATTCGCGCCGATA                                                                           >  SRR4302537.1314769/1‑94 (MQ=60)
                   ACAAAATAGGCGGCAATCATCAGCAGGTAAGGGGAGTTAATATACTGCAATGGTTTTGACGCCAGCTTGACCATCATATCATTCGCGCCGATAT                                                                          <  SRR4302537.4341669/94‑1 (MQ=60)
                   ACAAAATAGGCGGCAATCATCAGCAGGTAAGGGGAGTTAATATACTGCAATGGTTTTGACGCCAGCTTGACCATCATATCATTCGCGCCGATAT                                                                          <  SRR4302537.4667063/94‑1 (MQ=60)
                   ACAAAATAGGCGGCAATCATCAGCAGGTAAGGGGAGTTAATATACTGCAATGGTTTTGACGCCAGCTTGACCATCATATCATTCGCGCCGATAT                                                                          <  SRR4302537.617269/94‑1 (MQ=60)
                     AAAATAGGCGGCAATCATCAGCAGGTAAGGGGAGTTAATATACTGCAATGGTTTTGACGCCAGCTTGACCATCATATCATTCGCGCCGATATGG                                                                        <  SRR4302537.5185472/94‑1 (MQ=60)
                     AAAATAGGCGGCAATCATCAGCAGGTAAGGGGAGTTAATATACTGCAATGGTTTTGACGCCAGCTTGACCATCATATCATTCGCGCCGATATGG                                                                        >  SRR4302537.2688082/1‑94 (MQ=60)
                       AATAGGCGGCAATCATCAGCAGGTAAGGGGAGTTAATATACTGCAATGGTTTTGACGCCAGCTTGACCATCATATCATTCGCGCCGATATGGGT                                                                      >  SRR4302537.4126298/1‑94 (MQ=60)
                       AATAGGCGGCAATCATCAGCAGGTAAGGGGAGTTAATATACTGCAATGGTTTTGACGCCAGCTTGACCATCATATCATTCGCGCCGATATGGGT                                                                      >  SRR4302537.858676/1‑94 (MQ=60)
                        ATAGGCGGCAATCATCAGCAGGTAAGGGGAGTTAATATACTGCAATGGTTTTGACGCCAGCTTGACCATCATATCATTCGCGCCGATATGGGTC                                                                     <  SRR4302537.5924252/94‑1 (MQ=60)
                        ATAGGCGGCAATCATCAGCAGGTAAGGGGAGTTAATATACTGCAATGGTTTTGACGCCAGCTTGACCATCATATCATTCGCGCCGATATGGGTC                                                                     <  SRR4302537.346814/94‑1 (MQ=60)
                          AGGCGGCAATCATCAGCAGGTAAGGGGAGTTAATATACTGCAATGGTTTTGACGCCAGCTTGACCATCATATCATTCGCGCCGATATGGGTCAT                                                                   >  SRR4302537.2026383/1‑94 (MQ=60)
                          AGGCGGCAATCATCAGCAGGTAAGGGGAGTTAATATACTGCAATGGTTTTGACGCCAGCTTGACCATCATATCATTCGCGCCGATATGGGTCAT                                                                   >  SRR4302537.1754736/1‑94 (MQ=60)
                             CGGCAATCATCAGCAGGTAAGGGGAGTTAATATACTGCAATGGTTTTGACGCCAGCTTGACCATCATATCATTCGCGCCGATATGGGTCATGTA                                                                <  SRR4302537.1422248/94‑1 (MQ=60)
                                CAATCATCAGCAGGTAAGGGGAGTTAATATACTGCAATGGTTTTGACGCCAGCTTGACCATCATATCATTCGCGCCGATATGGGTCATGTAAGC                                                             >  SRR4302537.263054/1‑94 (MQ=60)
                                CAATCATCAGCAGGTAAGGGGAGTTAATATACTGCAATGGTTTTGACGCCAGCTTGACCATCATATCATTCGCGCCGATATGGGTCATGTAAGC                                                             <  SRR4302537.835842/94‑1 (MQ=60)
                                 AGTCATCAGCAGGTAAGCGGAGTTAATATCCTGCAATGGTTTTGACGCCAGCTTGACCAGCATAACATTGGCGCCGATATGGGTCATGTAAGCG                                                            <  SRR4302537.2540007/94‑1 (MQ=60)
                                 AATCATCAGCAGGTAAGGGGAGTTAATATACTGCAATGGTTTTGACGCCAGCTTGACCATCATATCATTCGCGCCGATATGGGTCATGTAAGCG                                                            <  SRR4302537.3966510/94‑1 (MQ=60)
                                 AATCATCAGCAGGTAAGGGGAGTTAATATACTGCAATGGTTTTGACGCCAGCTTGACCATCATATCATTCGCGCCGATATGGGTCATGTAAGCG                                                            <  SRR4302537.1244539/94‑1 (MQ=60)
                                 AATCATCAGCAGGTAAGGGGAGTTAATATACTGCAATGGTTTTGACGCCAGCTTGACCATCATATCATTCGCGCCGATATGGGTCATGTAAGCG                                                            >  SRR4302537.3768391/1‑94 (MQ=60)
                                 AATCATCAGCAGGTAAGGGGAGTTAATATACTGCAATGGTTTTGACGCCAGCTTGACCATCATATCATTCGCGCCGATATGGGTCATGTAAGCG                                                            <  SRR4302537.5678488/94‑1 (MQ=60)
                                  ATCATCAGCAGGTAAGGGGAGTTAATATACTGCAATGGTTTTGACGCCAGCTTGACCATCATATCATTCGCGCCGATATGGGTCATGTAAGCGG                                                           >  SRR4302537.2468122/1‑94 (MQ=60)
                                  ATCATCAGCAGGTAAGGGGAGTTAATATACTGCAATGGTTTTGACGCCAGCTTGACCATCATATCATTCGCGCCGATATGGGTCATGTAAGCGG                                                           >  SRR4302537.5095104/1‑94 (MQ=60)
                                  ATCATCAGCAGGTAAGGGGAGTTAATATACTGCAATGGTTTTGACGCCAGCTTGACCACCATATCATTCGCGCCGATATGGGTCATGTAAGCGG                                                           <  SRR4302537.286997/94‑1 (MQ=60)
                                   TCATCAGCAGGTAAGGGGAGTTAATATACTGCAATGGTTTTGACGCCAGCTTGACCATCATATCATTCGCGCCGATATGGGTCATGTAAGCGGC                                                          <  SRR4302537.872603/94‑1 (MQ=60)
                                   TCATCAGCAGGTAAGGGGAGTTAATATACTGCAATGGTTTTGACGCCAGCTTGACCATCATATCATTCGCGCCGATATGGGTCATGTAAGCGGC                                                          <  SRR4302537.3366608/94‑1 (MQ=60)
                                   TCATCAGCAGGTAAGGGGAGTTAATATACTGCAATGGTTTTGACGCCAGCTTGACCATCATATCATTCGCGCCGATATGGGTCATGTAAGCGGC                                                          <  SRR4302537.2936932/94‑1 (MQ=60)
                                   TCATCAGCAGGTAAGGGGAGTTAATATACTGCAATGGTTTTGACGCCAGCTTGACCATCATATCATTCGCGCCGATATGGGTCATGTAAGCGGC                                                          >  SRR4302537.2766936/1‑94 (MQ=60)
                                    CATCAGCAGGTAAGGGGAGTTAATATACTGCAATGGTTTTGACGCCAGCTTGACCATCATATCATTCGCGCCGATATGGGTCATGTAAGCGGCA                                                         <  SRR4302537.4952315/94‑1 (MQ=60)
                                     ATCAGCAGGTAAGGGGAGTTAATATACTGCAATGGTTTTGACGCCAGCTTGACCATCATATCATTCGCGCCGATATGGGTCATGTAAGCGGCAA                                                        >  SRR4302537.2261278/1‑94 (MQ=60)
                                       CAGCAGGTAAGGGGAGTTAATATACTGCAATGGTTTTGACGCCAGCTTGACCATCATATCATTCGCGCCGATATGGGTCATGTAAGCGGCAAAT                                                      <  SRR4302537.3512557/94‑1 (MQ=60)
                                        AGCAGGTAAGGGGAGTTAATATACTGCAATGGTTTTGACGCCAGCTTGACCATCATATCATTCGCGCCGATATGGGTCATGTAAGCGGCAAATC                                                     <  SRR4302537.361474/94‑1 (MQ=60)
                                        AGCAGGTAAGGGGAGTTAATATACTGCAATGGTTTTGACGCCAGCTTGACCATCATATCATTCGCGCCGATATGGGTCATGTAAGCGGCAAATC                                                     <  SRR4302537.3356520/94‑1 (MQ=60)
                                           AGGTAAGGGGAGTTAATATACTGCAATGGTTTTGACGCCAGCTTGACCATCATATCATTCGCGCCGATATGGGTCATGTAAGCGGCAAATCCAC                                                  >  SRR4302537.493344/1‑94 (MQ=60)
                                            GGTAAGGGGAGTTAATATACTGCAATGGTTTTGACGCCAGCTTGACCATCATATCATTCGCGCCGATATGGGTCATGTAAGCGGCAAATCCACA                                                 >  SRR4302537.4380097/1‑94 (MQ=60)
                                             GTAAGGGGAGTTAATATACTGCAATGGTTTTGACGCCAGCTTGACCATCATATCATTCGCGCCGATATGGGTCATGTAAGCGGCAAATCCACAC                                                >  SRR4302537.6354634/1‑94 (MQ=60)
                                              TAAGGGGAGTTAATATACTGCAATGGTTTTGACGCCAGCTTGACCATCATATCATTCGCGCCGATATGGGTCATGTAAGCGGCAAATCCACACA                                               <  SRR4302537.2562621/94‑1 (MQ=60)
                                               AAGGGGAGTTAATATACTGCAATGGTTTTGACGCCAGCTTGACCATCATATCATTCGCGCCGATATGGGTCATGTAAGCGGCAAATCCACACAG                                              >  SRR4302537.2484525/1‑94 (MQ=60)
                                                AGGGGAGTTAATATACTGCAATGGTTTTGACGCCAGCTTGACCATCATATCATTCGCGCCGATATGGGTCATGTAAGCGGCAAATCCACACAGC                                             >  SRR4302537.6357753/1‑94 (MQ=60)
                                                AGGGGAGTTAATATACTGCAATGGTTTTGACGCCAGCTTGACCATCATATCATTCGCGCCGATATGGGTCATGTAAGCGGCAAATCCACACAGC                                             >  SRR4302537.5623644/1‑94 (MQ=60)
                                                AGGGGAGTTAATATACTGCAATGGTTTTGACGCCAGCTTGACCATCATATCATTCGCGCCGATATGGGTCATGTAAGCGGCAAATCCACACAGC                                             <  SRR4302537.3839808/94‑1 (MQ=60)
                                                 GGGGAGTTAATATACTGCAATGGTTTTGACCCCAGCTTGACAATCTTATCATTCCCCCCGTTATGGGCCATGTAAGCGGCAAAccaacacacat                                            >  SRR4302537.4681609/1‑83 (MQ=44)
                                                  GGGAGTTAATATACTGCAATGGTTTTGACGCCAGCTTGACCATCATATCATTCGCGCCGATATGGGTCATGTAAGCGGCAAATCCACACAGCAT                                           <  SRR4302537.2215366/94‑1 (MQ=60)
                                                   GGAGTTAATATACTGCAATGGTTTTGACGCCAGCTTGACCATCATATCATTCGCGCCGATATGGGTCATGTAAGCGGCAAATCCACACAGCATC                                          <  SRR4302537.4508206/94‑1 (MQ=60)
                                                   GGAGTTAATATACTGCAATGGTTTTGACGCCAGCTTGACCATCATATCATTCGCGCCGATATGGGTCATGTAAGCGGCAAATCCACACAGCATC                                          >  SRR4302537.5486484/1‑94 (MQ=60)
                                                   GGAGTTAATATACTGCAATGGTTTTGACGCCAGCTTGACCATCATATCATTCGCGCCGATATGGGTCATGTAAGCGGCAAATCCACACAGCATA                                          >  SRR4302537.4694184/1‑94 (MQ=60)
                                                    GAGTTAATATACTGCAATGGTTTTGACGCCAGCTTGACCATCATATCATTCGCGCCGATATGGGTCATGTAAGCGGCAAATCCACACAGCATCA                                         >  SRR4302537.2650471/1‑94 (MQ=60)
                                                     AGTTAATATACTGCAATGGTTTTGACGCCAGCTTGACCATCATATCATTCGCGCCGATATGGGTCATGTAAGCGGCAAATCCACACAGCATCAT                                        >  SRR4302537.5656311/1‑94 (MQ=60)
                                                     AGTTAATATACTGCAATGGTTTTGACGCCAGCTTGACCATCATATCATTCGCGCCGATATGGGTCATGTAAGCGGCAAATCCACACAGCATCAT                                        <  SRR4302537.2302137/94‑1 (MQ=60)
                                                     AGTTAATATACTGCAATGGTTTTGACGCCAGCTTGACCATCATATCATTCGCGCCGATATGGGTCATGTAAGCGGCAAATCCACACAGCATCAT                                        <  SRR4302537.6277858/94‑1 (MQ=60)
                                                      GTTAATATACTGCAATGGTTTTGACGCCAGCTTGACCATCATATCATTCGCGCCGATATGGGTCATGTAAGCGGCAAATCCACACAGCATCATA                                       >  SRR4302537.5109780/1‑94 (MQ=60)
                                                       TTAATATACTGCAATGGTTTTGACGCCAGCTTGACCATCATATCATTCGCGCCGATATGGGTCATGTAAGCGGCAAATCCACACAGCATCATAA                                      <  SRR4302537.3812797/94‑1 (MQ=60)
                                                       TTAATATACTGCAATGGTTTTGACGCCAGCTTGACCACCATATCATTCGCGCCGATATGGGTCATGTAAGCGGCACATCCACACAGCATCATAA                                      >  SRR4302537.235572/1‑94 (MQ=60)
                                                         AATATACTGCAATGGTTTTGACGCCAGCTTGACCATCATATCATTCGCGCCGATATGGGTCATGTAAGCGGCAAATCCACACAGCATCATAATC                                    <  SRR4302537.4133830/94‑1 (MQ=60)
                                                          ATATACTGCAATGGTTTTGACGCCAGCTTGACCATCATATCATTCGCGCCGATATGGGTCATGTAAGCGGCAAATCCACACAGCATCATAATCA                                   >  SRR4302537.3674823/1‑94 (MQ=60)
                                                            ATACTGCAATGGTTTTGACGCCAGCTTGACCATCATATCATTCGCGCCGATATGGGTCATGTAAGCGGCAAATCCACACAGCATCATAATCATC                                 >  SRR4302537.3759007/1‑94 (MQ=60)
                                                               CTGCAATGGTTTTGACGCCAGCTTGACCATCATATCATTCTCGCCGATATGGGTCATGTAAGCGGCAAATCCACACAGCATCATAATCATCATG                              >  SRR4302537.2496868/1‑94 (MQ=60)
                                                               CTGCAATGGTTTTGACGCCAGCTTGACCATCATATCATTCGCGCCGATATGGGTCATGTAAGCGGCAAATCCACACAGCATCATAATCATCATG                              <  SRR4302537.450209/94‑1 (MQ=60)
                                                               ATGCAATGGTTTTGACGCCAGCTTGACCATCATATCATTCGCGCCGATATGGGTCATGTAAGCGGCAAATCCACACAGCATCATAATCATCATG                              <  SRR4302537.2189611/94‑1 (MQ=60)
                                                                TGCAATGGTTTTGACGCCAGCTTGACCATCATATCATTCGCGCCGATATGGGTCATGTAAGCGGCAAATCCACACAGCATCATAATCATCATGC                             >  SRR4302537.1997777/1‑94 (MQ=60)
                                                                  CAATGGTTTTGACGCCAGCTTGACCATCATATCATTCGCGCCGATATGGGTCATGTAAGCGGCAAATCCACACAGCATCATAATCATCATGCCG                           >  SRR4302537.6115199/1‑94 (MQ=60)
                                                                   AATGGTTTTGACGCCAGCTTGACCATCATATCATTCGCGCCGATATGGGTCATGTAAGCGGCAAATCCACACAGCATCATAATCATCATGCCGA                          >  SRR4302537.4993547/1‑94 (MQ=60)
                                                                    ATGGTTTTGACGCCAGCTTGACCATCATATCATTCGCGCCGATATGGGTCATGTAAGCGGCAAATCCACACAGCATCATAATCATCATGCCGAG                         <  SRR4302537.3574349/94‑1 (MQ=60)
                                                                      GGTTTTGACGCCAGCTTGACCATCATATCATTCGCGCCGATATGGGTCATGTAAGCGGCAAATCCACACAGCATCATAATCATCATGCCGAGGT                       <  SRR4302537.514079/94‑1 (MQ=60)
                                                                       GTTTTGACGCCAGCTTGACCATCATATCATTCGCGCCGATATGGGTCATGTAAGCGGCAAATCCACACAGCATCATAATCATCATGCCGAGGTC                      <  SRR4302537.2384244/94‑1 (MQ=60)
                                                                        TTTTGACGCCAGCTTGACCATCATATCATTCGCGCCGATATGGGTCATGTAAGCGGCAAATCCACACAGCATCATAATCATCATGCCGAGGTCG                     <  SRR4302537.1907641/94‑1 (MQ=60)
                                                                        TTTTGACGCCAGCTTGACCATCATATCATTCGCGCCGATATGGGTCATGTAAGCGGCAAATCCACACAGCATCATAATCATCATGCCGAGGTCG                     <  SRR4302537.5371792/94‑1 (MQ=60)
                                                                        TTTTGACGCCAGCTTGACCATCATATCATTCGCGCCGATATGGGTCATGTAAGCGGCAAATCCACACAGCATCATAATCATCATGCCGAGGTCG                     >  SRR4302537.2708465/1‑94 (MQ=60)
                                                                         TTTGACGCCAGCTTGACCATCATATCATTCGCGCCGATATGGGTCATGTAAGCGGCAAATCCACACAGCATCATAATCATCATGCCGAGGTCGC                    >  SRR4302537.5755310/1‑94 (MQ=60)
                                                                          TTGACGCCAGCTTGACCATCATATCATTCGCGCCGATATGGGTCATGTAAGCGGCAAATCCACACAGCATCATAATCATCATGCCGAGGTCGCC                   <  SRR4302537.4326878/94‑1 (MQ=60)
                                                                           TGACGCCAGCTTGACCATCATATCATTCGCGCCGATATGGGTCATGTAAGCGGCAAATCCACACAGCATCATAATCATCATGCCGAGGTCGCCG                  <  SRR4302537.1100473/94‑1 (MQ=60)
                                                                                CCAGCTTGACCATCATATCATTCGCGCCGATATGGGTCATGTAAGCGGCAAATCCACACAGCATCATAATCATCATGCCGAGGTCGCCGCCGCG             <  SRR4302537.5021183/94‑1 (MQ=60)
                                                                                CCAGCTTGACCATCATATCATTCGCGCCGATATGGGTCATGTAAGCGGCAAATCCACACAGCATCATAATCATCATGCCGAGGTCGCCGCCGCG             >  SRR4302537.2939935/1‑94 (MQ=60)
                                                                                 CAGCTTGACCATCATATCATTCGCGCCGATATGGGTCATGTAAGCGGCAAATCCACACAGCATCATAATCATCATGCCGAGGTCGCCGCCGCGG            >  SRR4302537.5050675/1‑94 (MQ=60)
                                                                                  AGCTTGACCATCATATCATTCGCGCCGATATGGGTCATGTAAGCGGCAAATCCACACAGCATCATAATCATCATGCCGAGGTCGCCGCCGCGGC           >  SRR4302537.3120995/1‑94 (MQ=60)
                                                                                   GCTTGACCATCATATCATTCGCGCCGATATGGGTCATGTAAGCGGCAAATCCACACAGCATCATAATCATCATGCCGAGGTCGCCGCCGCGGCT          >  SRR4302537.4718290/1‑94 (MQ=60)
                                                                                    CTTGACCATCATATCATTCGCGCCGATATGGGTCATGTAAGCGGCAAATCCACACAGCATCATAATCATCATGCCGAGGTCGCCGCCGCGGCTC         >  SRR4302537.134497/1‑94 (MQ=60)
                                                                                    CTTGACCATCATATCATTCGCGCCGATATGGGGCATGTAAGCGGCAAATCCACACAGCATCATAATCATCATGCCGAGGACGCCGCCGCGGCTC         >  SRR4302537.2035705/1‑94 (MQ=60)
                                                                                     TTGACCATCATATCATTCGCGCCGATATGGGTCATGTAAGCGGCAAATCCACACAGCATCATAATCATCATGCCGAGGTCGCCGCCGCGGCTCA        >  SRR4302537.5552761/1‑94 (MQ=60)
                                                                                      TGACCACCATATCATTCGCGCCGATATGGGTCATGTAAGCGGCAAATCCACACAGCATCATAATCATCATGCCGAGGTCGCCGCCGCGGCTCAT       <  SRR4302537.1175025/94‑1 (MQ=60)
                                                                                        ACCATCATATCATTCGCGCCGATATGGGTCATGTAAGCGGCAAATCCACACAGCATCATAATCATCATGACGAGGTCGCCGCCGCGGCTCATTA     <  SRR4302537.907864/94‑1 (MQ=60)
                                                                                         CCATCATATCATTCGCGCCGATATGGGTCATGTAAGCGGCAAATCCACACAGCATCATAATCATCATGCCGAGGTCGCCGCCGCGGCTCATTAG    <  SRR4302537.5426616/94‑1 (MQ=60)
                                                                                         CCATCATATCATTCGCGCCGATATGGGTCATGTAAGCGGCAAATCCACACAGCATCATAATCATCATGCCGAGGTCGCCGCCGCGGCTCATTAG    <  SRR4302537.291893/94‑1 (MQ=60)
                                                                                          CATCATATCATTCGCGCCGATATGGGTCATGTAAGCGGCAAATCCACACAGCATCATAATCATCATGCCGAGGTCGCCGCCGCGGCTCATTAGT   >  SRR4302537.2466656/1‑94 (MQ=60)
                                                                                          CATCATATCATTCGCGCCGATATGGGTCATGTAAGCGGCAAATCCACACAGCATCATAATCATCATGCCGAGGTCGCCGCCGCGGCTCATTAGT   <  SRR4302537.4316687/94‑1 (MQ=60)
                                                                                           ATCATATCATTCGCGCCGATATGGGTCATGTAAGCGGCAAATCCACACAGCATCATAATCATCATGCCGAGGTCTCCGCCGCGGCTCATTAGTA  >  SRR4302537.104539/1‑94 (MQ=60)
                                                                                           ATCATATCATTCGCGCCGATATGGGTCATGTAAGCGGCAAATCCACACAGCATCATAATCATCATGCCGAGGTCGCCGCCGCGGCTCATTAGTA  >  SRR4302537.4290913/1‑94 (MQ=60)
                                                                                           ATCATATCATTCGCGCCGATATGGGTCATGTAAGCGGCAAATCCACACAGCATCATAATCATCATGCCGAGGTCGCCGCCGCGGCTCATTAGTA  >  SRR4302537.1255172/1‑94 (MQ=60)
                                                                                           ATCATATCATTCGCGCCGATATGGGTCATGTAAGCGGCAAATCCACACAGCATCATAATCATCATGCCGAGGTCGCCGCCGCGGCTCATTAGTA  <  SRR4302537.6073124/94‑1 (MQ=60)
                                                                                           ATCATATAATTCGCGCCGATATGGGTCATGTAAGCGGCAAATCCACACAGCATCATAATCATCATGCCGAGGTCGCCGCCGCGGCTCATTAGTA  >  SRR4302537.2975210/1‑94 (MQ=60)
                                                                                            |                                                                                            
CAGAGACATCAGACAGGCGACAAAATAGGCGGCAATCATCAGCAGGTAAGGGGAGTTAATATACTGCAATGGTTTTGACGCCAGCTTGACCACCATATCATTCGCGCCGATATGGGTCATGTAAGCGGCAAATCCACACAGCATCATAATCATCATGCCGAGGTCGCCGCCGCGGCTCATTAGTA  >  NC_000913/654796‑654980

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑