Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I2 R1 882 254.4 12957588 98.0% 12698436 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,317,682 T→C E56G (GAG→GGG)  rcsC ← RcsC

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,317,6820TC96.6% 677.3 / 8.1 208E56G (GAG→GGG) rcsCRcsC
Reads supporting (aligned to +/- strand):  ref base T (2/5);  new base C (94/107);  total (96/112)
Fisher's exact test for biased strand distribution p-value = 4.55e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.97e-01

Only 100 of 213 total aligned reads displayed.

AACGCAGGCACATCCGCCTGCGTTTCTCGTCCACGCGGGGAAAGCACACCGTTTTCTGCCGATAAGCGATTTTCGGCGATGTACTTCAGCTCTTTCATCACATCAGAGGTGCGTTGAATAAAGCGCTGAGCCTGATCGGAACTCAGATTAAATTCCTGACGAATTTCCGATTCTCGCTGA  >  NC_000913/2317592‑2317771
                                                                                          |                                                                                         
aaCGCAGGCACATCCGCCTGCGTTTCTCGTCCACGCGGGGAAAGCACACCGTTTTCTGCCGATAAGCGATTTTCGGCGATGTACTTCAGctct                                                                                         <  1:4210936/93‑1 (MQ=255)
aaCGCAGGCACATCCGCCTGCGTTTCTCGTCCACGCGGGGAAAGCACACCGTTTTCTGCCGATAAGCGATTTTCGGCGATGTACTTCAGccct                                                                                         >  2:4556928/1‑93 (MQ=255)
 aCGCAGGCACATCCGCCTGCGTTTCTCGTCCACGCGGGGAAAGCACACCGTTTTCTGCCGATAAGCGATTTTCGGCGATGTACTTCAGCCCtt                                                                                        <  2:2148319/93‑1 (MQ=255)
 aCGCAGGCACATCCGCCTGCGTTTCTCGTCCACGCGGGGAAAGCACACCGTTTTCTGCCGATAAGCGATTTTCGGCGATGTACTTCAGCCCtt                                                                                        <  2:3284813/93‑1 (MQ=255)
 aCGCAGGCACATCCGCCTGCGTTTCTCGTCCACGCGGGGAAAGCACACCGTTTTCTGCCGATAAGCGATTTTCGGCGATGTACTTCAGCCCtt                                                                                        >  2:2879716/1‑93 (MQ=255)
 aCGCAGGCACATCCGCCTGCGTTTCTCGTCCACGCGGGGAAAGCACACCGTTTTCTGCCGATAAGCGATTTTCGGCGATGTACTTCAGCCCtt                                                                                        <  1:4632785/93‑1 (MQ=255)
  cGCAGGCACATCCGCCTGCGTTTCTCGTCCACGCGGGGAAAGCACACCGTTTTCTGCCGATAAGCGATTTTCGGCGATGTACTTCAGCCCttt                                                                                       >  2:1444224/1‑93 (MQ=255)
    cAGGCACATCCGCCTGCGTTTCTCGTCCACGCGGGGAAAGCACACCGTTTTCTGCCGATAAGCGATTTTCGGCGATGTACTTCAGCCCTTtca                                                                                     >  1:1792961/1‑93 (MQ=255)
    cAGGCACATCCGCCTGCGTTTCTCGTCCACGCGGGGAAAGCACACCGTTTTCTGCCGATAAGCGATTTTCGGCGATGTACTTCAGCCCTTtca                                                                                     <  2:2808384/93‑1 (MQ=255)
        cacaTCCGCCTGCGTTTCTCGTCCACGCGGGGAAAGCACACCGTTTTCTGCCGATAAGCGATTTTCGGCGATGTACTTCAGCCCTTTcatcac                                                                                 >  1:2746269/1‑93 (MQ=255)
         acaTCCGCCTGCGTTTCTCGTCCACGCGGGGAAAGCACACCGTTTTCTGCCGATAAGCGATTTTCGGCGATGTACTTCAGCCCTTTcatcaca                                                                                >  2:4997722/1‑93 (MQ=255)
          caTCCGCCTGCGTTTCTCGTCCACGCGGGGAAAGCACACCGTTTTCTGCCGATAAGCGATTTTCGGCGATGTACTTCAGCCCTTTcatcacat                                                                               >  2:4136118/1‑93 (MQ=255)
          caTCCGCCTGCGTTTCTCGTCCACGCGGGGAAAGCACACCGTTTTCTGCCGATAAGCGATTTTCGGCGATGTACTTCAGCCCTTTcatcacat                                                                               <  2:1667612/93‑1 (MQ=255)
          caTCCGCCTGCGTTTCTCGTCCACGCGGGGAAAGCACACCGTTTTCTGCCGATAAGCGATTTTCGGCGATGTACTTCAGCCCTTTcatcacat                                                                               <  2:3184112/93‑1 (MQ=255)
           aTCCGCCTGCGTTTCTCGTCCACGCGGGGAAAGCACACCGTTTTCTGCCGATAAGCGATTTTCGGCGATGTACTTCAGCCCTTTcatcacatc                                                                              <  1:4269975/93‑1 (MQ=255)
           aTCCGCCTGCGTTTCTCGTCCACGCGGGGAAAGCACACCGTTTTCTGCCGATAAGCGATTTTCGGCGATGTACTTCAGCCCTTTcatcacatc                                                                              >  2:6034790/1‑93 (MQ=255)
            tCCGCCTGCGTTTCTCGTCCACGCGGGGAAAGCACACCGTTTTCTGCCGATAAGCGATTTTCGGCGATGTACTTCAGCCCTTTcatcacatca                                                                             <  2:5442554/93‑1 (MQ=255)
             ccGCCTGCGTTTCTCGTCCACGCGGGGAAAGCACACCGTTTTCTGCCGATAAGCGATTTTCGGCGATGTACTTCAGCCCTTTCATCACATCag                                                                            <  1:471941/93‑1 (MQ=255)
              cGCCTGCGTTTCTCGTCCACGCGGGGAAAGCACACCGTTTTCTGCCGATAAGCGATTTTCGGCGATGTCCTTCAGCCCTTTCATCACATCaga                                                                           <  2:1924384/93‑1 (MQ=255)
              cGCCTGCGTTTCTCGTCCACGCGGGGAAAGCACACCGTTTTCTGCCGATAAGCGATTTTCGGCGATGTACTTCAGCCCTTTCATCACATCaga                                                                           >  2:4084461/1‑93 (MQ=255)
              cGCCTGCGTTTCTCGTCCACGCGGGGAAAGCACACCGTTTTCTGCCGATAAGCGATTTTCGGCGATGTACTTCAGCCCTTTCATCACATCaga                                                                           <  2:3669366/93‑1 (MQ=255)
              cGCCTGCGTTTCTCGTCCACGCGGGGAAAGCACACCGTTTTCTGCCGATAAGCGATTTTCGGCGATGTACTTCAGCCCTTTCATCACATCaga                                                                           <  2:4481833/93‑1 (MQ=255)
              cGCCTGCGTGTCTCGTCCACGCGTGGAAAGCACACCGTTTTCTGCCGATAAGCGATTTTCGGCGATGTACTTCAGCCCTTTCATCACATCaga                                                                           <  2:2113988/93‑1 (MQ=255)
                ccTGCGTTTCTCGTCCACGCGGGGAAAGCACACCGTTTTCTGCCGATAAGCGATTTTCGGCGATGTACTTCAGCCCTTTCATCACATCAGAgg                                                                         >  1:1497024/1‑93 (MQ=255)
                 cTGCGTTTCTCGTCCACGCGGGGAAAGCACACCGTTTTCTGCCGATAAGCGATTTTCGGCGATGTACTTCAGCCCTTTCATCACATCAGAGGt                                                                        >  1:3749994/1‑93 (MQ=255)
                 cTGCGTTTCTCGTCCACGCGGGGAAAGCACACCGTTTTCTGCCGATAAGCGATTTTCGGCGATGTACTTCAGCCCTTTCATCACATCAGAGGt                                                                        <  2:2634400/93‑1 (MQ=255)
                  tGCGTTTCTCGTCCACGCGGGGAAAGCACACCGTTTTCTGCCGATAAGCGATTTTCGGCGATGTACTTCAGCCCTTTCATCACATCAGAGGTg                                                                       >  2:420632/1‑93 (MQ=255)
                  tGCGTTTCTCGTCCACGCGGGGAAAGCACACCGTTTTCTGCCGATAAGCGATTTTCGGCGATGTACTTCAGCCCTTTCATCACATCAGAGGTg                                                                       >  2:4765786/1‑93 (MQ=255)
                   gCGTTTCTCGTCCACGCGGGGAAAGCACACCGTTTTCTGCCGATAAGCGATTTTCGGCGATGTACTTCAGCCCTTTCATCACATCAGAGGTgg                                                                      >  2:596943/1‑92 (MQ=255)
                    cGTTTCTCGTCCACGCGGGGAAAGCACACCGTTTTCTGCCGATAAGCGATTTTCGGCGATGTACTTCAGCCCTTTCATCACATCAGAGGTGCg                                                                     >  2:3146051/1‑93 (MQ=255)
                     gTTTCTCGTCCACGCGGGGAAAGCACACCGTTTTCTGCCGATAAGCGATTTTCGGCGATGTACTTCAGCTCTTTCATCACATCAGAGGTGCGt                                                                    <  1:3944346/93‑1 (MQ=255)
                        tctcGTCCACGCGGGGAAAGCACACCGTTTTCTGCCGATAAGCGATTTTCGGCGATGTACTTCAGCCCTTTCATCACATCAGAGGTGCGTTGa                                                                 <  1:836855/93‑1 (MQ=255)
                         ctcGTCCACGCGGGGAAAGCACACCGTTTTCTGCCGATAAGCGATTTTCGGCGATGTACTTCAGCCCTTTCATCACATCAGAGGTGCGTTGaa                                                                <  1:6035857/93‑1 (MQ=255)
                         ctcGTCCACGCGGGGAAAGCACACCGTTTTCTGCCGATAAGCGATTTTCGGCGATGTACTTCAGCCCTTTCATCACATCAGAGGTGCGTTGaa                                                                <  1:527485/93‑1 (MQ=255)
                         ctcGTCCACGCGGGGAAAGCACACCGTTTTCTGCCGATAAGCGATTTTCGGCGATGTACTTCAGCCCTTTCATCACATCAGAGGTGCGTTGaa                                                                <  1:6074168/93‑1 (MQ=255)
                          tcGTCCACGCGGGGAAAGCACACCGTTTTCTGCCGATAAGCGATTTTCGGCGATGTACTTCAGCCCTTTCATCACATCAGAGGTGCGTTGAAt                                                               <  1:4444108/93‑1 (MQ=255)
                          tcGTCCACGCGGGGAAAGCACACCGTTTTCTGCCGATAAGCGATTTTCGGCGATGTACTTCAGCCCTTTCATCACATCAGAGGTGCGTTGAAt                                                               <  1:495064/93‑1 (MQ=255)
                           cGTCCACGCGGGGAAAGCACACCGTTTTCTGCCGATAAGCGATTTTCGGCGATGTACTTCAGCCCTTTCATCACATCAGAGGTGCGTTGAATa                                                              >  2:3652563/1‑93 (MQ=255)
                            gTCCACGCGGGGAAAGCACACCGTTTTCTGCCGATAAGCGATTTTCGGCGATGTACTTCAGCCCTTTCATCACATCAGAGGTGCGTTGAATaa                                                             >  2:578407/1‑93 (MQ=255)
                            gTCCACGCGGGGAAAGCACACCGTTTTCTGCCGATAAGCGATTTTCGGCGATGTACTTCAGCCCTTTCATCACATCAGAGGTGCGTTGAATaa                                                             >  1:910688/1‑93 (MQ=255)
                              ccACGCGGGGAAAGCACACCGTTTTCTGCCGATAAGCGATTTTCGGCGATGTACTTCAGCCCTTTCATCACATCAGAGGTGCGTTGAATAAAg                                                           <  1:6043500/93‑1 (MQ=255)
                               cACGCGGGGAAAGCACACCGTTTTCTGCCGATAAGCGATTTTCGGCGATGTACTTCAGCCCTTTCATCACATCAGAGGTGCGTTGAATAAAgc                                                          <  2:2256319/93‑1 (MQ=255)
                               cACGCGGGGAAAGCACACCGTTTTCTGCCGATAAGCGATTTTCGGCGATGTACTTCAGCCCTTTCATCACATCAGAGGTGCGTTGAATAAAgc                                                          <  2:4032634/93‑1 (MQ=255)
                                 cgcgGGGAAAGCACACCGTTTTCTGCCGATAAGCGATTTTCGGCGATGTACTTCAGCCCTTTCATCACATCAGAGGTGCGTTGAATAAAgcgc                                                        <  1:5081924/93‑1 (MQ=255)
                                 cgcgGGGAAAGCACACCGTTTTCTGCCGATAAGCGATTTTCGGCGATGTACTTCAGCCCTTTCATCACATCAGAGGTGCGTTGAATAAAgcgc                                                        <  1:4764364/93‑1 (MQ=255)
                                   cgGGGAAAGCACACCGTTTTCTGCCGATAAGCGATTTTCGGCGATGTACTTCAGCCCTTTCATCACATCAGAGGTGCGTTGAATAAAGCGCTg                                                      >  1:2822909/1‑93 (MQ=255)
                                      ggAAAGCACACCGTTTTCTGCCGATAAGCGATTTTCGGCGATGTACTTCAGCCCTTTCATCACATCAGAGGTGCGTTGAATAAAGCGCTGAGc                                                   <  2:5164634/93‑1 (MQ=255)
                                        aaaGCACACCGTTTTCTGCCGATAAGCGATTTTCGGCGATGTACTTCAGCCCTTTCATCACATCAGAGGTGCGTTGAATAAAGCGCTGAGCCt                                                 <  1:5728905/93‑1 (MQ=255)
                                          aGCACACCGTTTTCTGCCGATAAGCGATTTTCGGCGATGTACTTCAGCCCTTTCATCACATCAGAGGTGCGTTGAATAAAGCGCTGAGCCTGa                                               >  1:3378861/1‑93 (MQ=255)
                                           gCACACCGTTTTCTGCCGATAAGCGATTTTCGGCGATGTACTTCAGCCCTTTCATCACATCAGAGGTGCGTTGAATAAAGCGCTGAGCCTGAt                                              >  1:1959660/1‑93 (MQ=255)
                                           gCACACCGTTTTCTGCCGATAAGCGATTTTCGGCGATGTACTTCAGCCCTTTCATCACATCAGAGGTGCGTTGAATAAAGCGCTGAGCCTGAt                                              <  1:5889098/93‑1 (MQ=255)
                                             acacCGTTTTCTGCCGATAAGCGATTTTCGGCGATGTACTTCAGCCCTTTCATCACATCAGAGGTGCGTTGAATAAAGCGCTGAGCCTGATCg                                            >  1:6436382/1‑93 (MQ=255)
                                             acacCGTTTTCTGCCGATAAGCGATTTTCGGCGATGTACTTCAGCCCTTTCATCACATCAGAGGTGCGTTGAATAAAGCGCTGAGCCTGATCg                                            >  2:1043553/1‑93 (MQ=255)
                                                ccGTTTTCTGCCGATAAGCGATTTTCGGCGATGTACTTCAGCCCTTTCATCACATCAGAGGTGCGTTGAATAAAGCGCTGAGCCTGATCGGaa                                         >  2:1103661/1‑93 (MQ=255)
                                                 cGTTTTCTGCCGATAAGCGATTTTCGGCGATGTACTTCAGCCCTTTCATCACATCAGAGGTGCGTTGAATAAAGCGCTGAGCCTGATCGGAAc                                        <  1:625059/93‑1 (MQ=255)
                                                   ttttCTGCCGATAAGCGATTTTCGGCGATGTACTTCAGCCCTTTCATCACATCAGAGGTGCGTTGAATAAAGCGCTGAGCCTGATCGGAACTc                                      >  1:3206007/1‑93 (MQ=255)
                                                   ttttCTGCCGATAAGCGATTTTCGGCGATGTACTTCAGCCCTTTCATCACATCAGAGGTGCGTTGAATAAAGCGCTGAGCCTGATCGGAACTc                                      <  1:2476821/93‑1 (MQ=255)
                                                    tttCTGCCGATAAGCGATTTTCGGCGATGTACTTCAGCCCTTTCATCACATCAGAGGTGCGTTGAATAAAGCGCTGAGCCTGCTCGGAACTCa                                     >  2:76187/1‑93 (MQ=255)
                                                     ttCTGCCGATAAGCGATTGTCGGCGATGTACTTTAGCCCTTTCATCACATCAGAGGTGCGTTGAATAAAGCGCTGAGCCTGATCGGAACTCAg                                    <  2:35578/93‑1 (MQ=255)
                                                       cTGCCGATAAGCGATTTTCGGCGATGTACTTCAGCCCTTTCATCACATCAGAGGTGCGTTGAATAAAGCGCTGAGCCTGATCGGAACTCAGAt                                  <  1:824079/93‑1 (MQ=255)
                                                          ccGATAAGCGATTGTCGGCGATGTACTTCAGCTCTTTCATCACATCAGAGGTGCGTTGAATAAAGCGCTGAGCCTGATCGGAACTCAGATTaa                               <  2:5699567/93‑1 (MQ=255)
                                                           cGATAAGCGATTTTCGGCGATGTACTTCAGCCCTTTCATCACATCAGAGGTGCGTTGAATAAAGCGCTGAGCCTGATCGGAACTCAGATTaaa                              <  1:3788890/93‑1 (MQ=255)
                                                            gATAAGCGATTTTCGGCGATGTACTTCAGCCCTTTCATCACATCAGAGGTGCGTTGAATAAAGCGCTGAGCCTGATCGGAACTCAGATTAAAt                             >  1:5463688/1‑93 (MQ=255)
                                                             aTAAGCGATTTTCGGCGATGTACTTCAGCCCTTTCATCACATCAGAGGTGCGTTGAATAAAGCGCTGAGCCTGATCGGAACTCAGATTAAAtt                            >  1:2586560/1‑93 (MQ=255)
                                                             aTAAGCGATTTTCGGCGATGTACTTCAGCCCTTTCATCACATCAGAGGTGCGTTGAATAAAGAGCTGAGCCTGATCGGAACTCAGATTAAAtt                            >  1:3578583/1‑93 (MQ=255)
                                                              tAAGCGATTTTCGGCGATGTACTTCAGCCCTTTCATCACATCAGAGGTGCGTTGAATAAAGCGCTGAGCCTGATCGGAACTCAGATTAAATTc                           <  1:4590851/93‑1 (MQ=255)
                                                                  cGATTTTCGGCGATGTACTTCAGCCCTTTCATCACATCAGAGGTGCGTTGAATAAAGCGCTGAGCCTGATCGGAACTCAGATTAAATTCCTGa                       <  1:886933/93‑1 (MQ=255)
                                                                   gATTTTCGGCGATGTACTTCAGCCCTTTCATCACATCAGAGGTGCGTTGAATAAAGCGCTGAGCCTGATCGGAACTCAGATTAAATTCCTGAc                      <  1:948452/93‑1 (MQ=255)
                                                                   gATTTTCAGCGATGTACTTCAGCCCTTTCATCACATCAGAGGTGCGTTGAATAAAGCGCTGAGCCTGATCGGAACTCAGATTAAATTCCTGAc                      >  2:6137513/1‑93 (MQ=255)
                                                                    aTTTTCGGCGATGTACTTCAGCCCTTTCATCACATCAGAGGTGCGTTGAATAAAGCGCTGAGCCTGATCGGAACTCAGATTAAATTCCTGACg                     >  2:2185361/1‑93 (MQ=255)
                                                                    aTTTTCGGCGATGTACTTCAGCCCTTTCATCACATCAGAGGTGCGTTGAATAAAGCGCTGAGCCTGATCGGAACTCAGATTAAATTCCTGACg                     >  1:1554969/1‑93 (MQ=255)
                                                                    aTTTTCGGCGATGTACTTCAGCCCTTTCATCACATCAGAGGTGCGTTGAATAAAGCGCTGAGCCTGATCGGAACTCAGATTAAATTCCTGACg                     >  1:5191759/1‑93 (MQ=255)
                                                                     ttttCGGCGATGTACTTCAGCCCTTTCATCACATCAGAGGTGCGTTGAATAAAGCGCTGAGCCTGATCGGAACTCAGATTAAATTCCTGACGa                    >  2:3902438/1‑93 (MQ=255)
                                                                       ttCGGCGATGTACTTCAGCCCTTTCATCACATCAGAGGTGCGTTGAATAAAGCGCTGAGCCTGATCGGAACTCAGATTAAATTCCTGACGAAt                  <  2:2079766/93‑1 (MQ=255)
                                                                          ggCGATGTACTTCAGCCCTTTCATCACATCAGAGGTGCGTTGAATAAAGCGCTGAGCCTGATCGGAACTCAGATTAAATTCCTGACGAATTTc               <  2:1471943/93‑1 (MQ=255)
                                                                          ggCGATGTACTTCAGCCCTTTCATCACATCAGAGGTGCGTTGAATAAAGCGCTGAGCCTGATCGGAACTCAGATTAAATTCCTGACGAATTTc               <  1:3665672/93‑1 (MQ=255)
                                                                          ggCGATGTACTTCAGCCCTTTCATCACATCAGAGGTGCGTTGAATAAAGCGCTGAGCCTGATCGGAACTCAGATTAAATTCCTGACGAATTTc               <  2:3432874/93‑1 (MQ=255)
                                                                             gATGTACTTCAGCCCTTTCATCACATCAGAGGTGCGTTGAATAAAGCGCTGAGCCTGATCGGAACTCAGATTAAATTCCTGACGAATTTCCGa            <  1:2171907/93‑1 (MQ=255)
                                                                             gATGTACTTCAGCCCTTTCATCACATCAGAGGTGCGTTGAATAAAGCGCTGAGCCTGATCGGAACTCAGATTAAATTCCTGACGAATTTCCGa            >  1:5935054/1‑93 (MQ=255)
                                                                              aTGTACTTCAGCCCTTTCATCACATCAGAGGTGCGTTGAATAAAGCGCTGAGCCTGATCGGAACTCAGATTAAATTCCTGACGAATTTCCGAt           >  2:207060/1‑93 (MQ=255)
                                                                              aTGTACTTCAGCCCTTTCATCACATCAGAGGTGCGTTGAATAAAGCGCTGAGCCTGATCGGAACTCAGATTAAATTCCTGACGAATTTCCGAt           <  1:2087860/93‑1 (MQ=255)
                                                                              aTGTACTTCAGCCCTTTCATCACATCAGAGGTGCGTTGAATAAAGCGCTGAGCCTGATCGGAACTCAGATTAAATTCCTGACGAATTTCCGAt           >  2:1284922/1‑93 (MQ=255)
                                                                               tGTACTTCAGCCCTTTCATCACATCAGAGGTGCGTTGAATAAAGCGCTGAGCCTGATCGGAACTCAGATTAAATTCCTGACGAATTTCCGAtt          >  1:5815434/1‑93 (MQ=255)
                                                                               tGTACTTCAGCCCTTTCATCACATCAGAGGTGCGTTGAATAAAGCGCTGAGCCTGATCGGAACTCAGATTAAATTCCTGACGAATTTCCGAtt          >  1:2194250/1‑93 (MQ=255)
                                                                                gTACTTCAGCCCTTTCATCACATCAGAGGTGCGTTGAATAAAGCGCTGAGCCTGATCGGAACTCAGATTAAATTCCTGACGAATTTCCGATtc         >  1:3531445/1‑93 (MQ=255)
                                                                                gTACTTCAGCCCTTTCATCACATCAGAGGTGCGTTGAATAAAGCGCTGAGCCTGATCGGAACTCAGATTAAATTCCTGACGAATTTCCGATtc         <  1:2907045/93‑1 (MQ=255)
                                                                                gTACTTCAGCCCTTTCATCACATCAGAGGTGCGTTGAATAAAGCGCTGAGCCTGATCGGAACTCAGATTAAATTCCTGACGAATTTCCGATtc         <  2:5296545/93‑1 (MQ=255)
                                                                                 tACTTCAGCCCTTTCATCACATCAGAGGTGCGTTGAATAAAGCGCTGAGCCTGATCGGAACTCAGATTAAATTCCTGACGAATTTCCGATtct        <  1:1673979/93‑1 (MQ=255)
                                                                                 tACTTCAGCCCTTTCATCACATCAGAGGTGCGTTGAATAAAGCGCTGAGCCTGATCGGAACTCAGATTAAATTCCTGACGAATTTCCGATtct        <  2:6257580/93‑1 (MQ=255)
                                                                                  tcatCAGCCCCTTCAACACATCAGAGGTGCGTTGAATAAAGCGCTGAGCCTGATCGGAACTCAGATTAAATTCCTGACTAATTTCCGATtctc       <  1:1183030/90‑1 (MQ=255)
                                                                                  aCTTCAGCCCTTTCATCACATCAGAGGTGCGTTGAATAAAGCGCTGAGCCTGATCGGAACTCAGATTAAATTCCTGACGAATTTCCGATtctc       >  2:1748040/1‑93 (MQ=255)
                                                                                  aCTTCAGCCCTTTCATCACATCAGAGGTGCGTTGAATAAAGCGCTGAGCCTGATCGGAACTCAGATTAAATTCCTGACGAATTTCCGATtctc       <  1:4075206/93‑1 (MQ=255)
                                                                                   cTTCAGCCCTTTCATCACATCAGAGGTGCGTTGAATAAAGCGCTGAGCCTGATCGGAACTCAGATTAAATTCCTGACGAATTTCCGATTCTCg      >  1:4798093/1‑93 (MQ=255)
                                                                                    ttCAGCCCTTTCATCACATCAGAGGTGCGTTGAATAAAGCGCTGAGCCTGATCGGAACTCAGATTAAATTCCTGACGAATTTCCGATTCTCGc     >  1:3702945/1‑93 (MQ=255)
                                                                                     tCAGCCCTTTCATCACATCAGAGGTGCGTTGAATAAAGCGCTGAGCCTGATCGGAACTCAGATTAAATTCCTGACGAATTTCCGATTCTCGCt    >  2:4853132/1‑93 (MQ=255)
                                                                                     tCAGCCCTTTCATCACATCAGAGGTGCGTTGAATAAAGCGCTGAGCCTGATCGGAACTCAGATTAAATTCCTGACGAATTTCCGATTCTCGCt    >  1:183946/1‑93 (MQ=255)
                                                                                     tCAGCCCTTTCATCACATCAGAGGTGCGTTGAATAAAGCGCTGAGCCTGATCGGAACTCAGATTAAATTCCTGACGAATTTCCGATTCTCGCt    <  1:1340703/93‑1 (MQ=255)
                                                                                       aGCCCTTTCATCACATCAGAGGTGCGTTGAATAAAGCGCTGAGCCTGATCGGAACTCAGATTAAATTCCTGACGAATTTCCGATTCTCGCTGa  >  2:2217207/1‑93 (MQ=255)
                                                                                       aGCCCTTTCATCACATCAGAGGTGCGTTGAATAAAGCGCTGAGCCTGATCGGAACTCAGATTAAATTCCTGACGAATTTCCGATTCTCGCTGa  <  2:126275/93‑1 (MQ=255)
                                                                                       aGCCCTTTCATCACATCAGAGGTGCGTTGAATAAAGCGCTGAGCCTGATCGGAACTCAGATTAAATTCCTGACGAATTTCCGATTCTCGCTGa  >  2:2963335/1‑93 (MQ=255)
                                                                                          |                                                                                         
AACGCAGGCACATCCGCCTGCGTTTCTCGTCCACGCGGGGAAAGCACACCGTTTTCTGCCGATAAGCGATTTTCGGCGATGTACTTCAGCTCTTTCATCACATCAGAGGTGCGTTGAATAAAGCGCTGAGCCTGATCGGAACTCAGATTAAATTCCTGACGAATTTCCGATTCTCGCTGA  >  NC_000913/2317592‑2317771

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
TCAAACGCAGGCACATCCGCCTGCGTTTCTCGTCCACGCGGGGAAAGCACACCGTTTTCTGCCGATAAGCGATTTTCGGCGATGTACTTCAGCTCTTTCATCACATCAGAGGTGCGTTGAATAAAGCGCTGAGCCTGATCGGAACTCAGATTAAATTCCTGACGAATTTCCGATTCTCGCTGATGTA  >  NC_000913/2317589‑2317775
                                                                                             |                                                                                             
TCAAACGCAGGCACATCCGCCTGCGTTTCTCGTCCACGCGGGGAAAGCACACCGTTTTCTGCCGATAAGCGATTTTCGGCGATGTACTTCAGCC                                                                                               <  SRR4302537.5383039/94‑1 (MQ=60)
TCAAACGCAGGCACATCCGCCTGCGTTTCTCGTCCACGCGGGGAAAGCACACCGTTTTCTGCCGATAAGCGATTTTCGGCGATGTACTTCAGCC                                                                                               >  SRR4302537.5203745/1‑94 (MQ=60)
  AAACGCAGGCACATCCGCCTGCGTTTCTCGTCCACGCGGGGAAAGCACACCGTTTTCTGCCGATAAGCGATTTTCGGCGATGTACTTCAGCCCT                                                                                             <  SRR4302537.3524053/94‑1 (MQ=60)
   AACGCAGGCACATCCGCCTGCGTTTCTCGTCCACGCGGGGAAAGCACACCGTTTTCTGCCGATAAGCGATTTTCGGCGATGTACTTCAGCTCTT                                                                                            <  SRR4302537.4240307/94‑1 (MQ=60)
    ACGCAGGCACATCCGCCTGCGTTTCTCGTCCACGCGGGGAAAGCACACCGTTTTCTGCCGATAAGCGATTTTCGGCGATGTACTTCAGCCCTTT                                                                                           <  SRR4302537.4664834/94‑1 (MQ=60)
     CGCAGGCACATCCGCCTGCGTTTCTCGTCCACGCGGGGAAAGCACACCGTTTTCTGCCGATAAGCGATTTTCGGCGATGTACTTCAGCCCTTTC                                                                                          >  SRR4302537.911335/1‑94 (MQ=60)
     CACAGGCACATCCGCCTGCGTTTCTCGTCCACGCGGGGAAAGCACACCGTTTTCTGCCGATAAGCGATTTTCGGCGATGTACTTCAGCTCTTTC                                                                                          <  SRR4302537.918544/94‑1 (MQ=60)
      GCAGGCACATCCGCCTGCGTTTCTCGTCCACGCGGGGAAAGCACACCGTTTTCTGCCGATAAGCGATTTTCGGCGATGTACTTCAGCCCTTTCA                                                                                         >  SRR4302537.1805474/1‑94 (MQ=60)
       CAGGCACATCCGCCTGCGTTTCTCGTCCACGCGGGGAAAGCACACCGTTTTCTGCCGATAAGCGATTTTCGGCGATGTACTTCAGCCCTTTCAT                                                                                        <  SRR4302537.5155354/94‑1 (MQ=60)
        AGGCACATCCGCCTGCGTTTCTCGTCCACGCGGGGAAAGCACACCGTTTTCTGCCGATAAGCGATTTTCGGCGATGTACTTCAGCCCTTTCATC                                                                                       >  SRR4302537.4248678/1‑94 (MQ=60)
         GGCACATCCGCCTGCGTTTCTCGTCCACGCGGGGAAAGCACACCGTTTTCTGCCGATAAGCGATTTTCGGCGATGTACTTCAGCCCTTTCATCA                                                                                      >  SRR4302537.3791144/1‑94 (MQ=60)
          GCACATCCGCCTGCGTTTCTCGTCCACGCGGGGAAAGCACACCGTTTTCTGCCGATAAGCGATTTTCGGCGATGTACTTCAGCCCTTTCATCAC                                                                                     >  SRR4302537.2765423/1‑94 (MQ=60)
           CACATCCGCCTGCGTTTCTCGTCCACGCGGGGAAAGCACACCGTTTTCTGCCGATAAGCGATTTTCGGCGATGTACTTCAGCCCTTTCATCACA                                                                                    >  SRR4302537.2193756/1‑94 (MQ=60)
            ACATCCGCCTGCGTTTCTCGTCCACGCGGGGAAAGCACACCGTTTTCTGCCGATAAGCGATTTTCGGCGATGTACTTCAGCCCTTTCATCACAT                                                                                   >  SRR4302537.5457803/1‑94 (MQ=60)
             CATCCGCCTGCGTTTCTCGTCCACGCGGGGAAAGCACACCGTTTTCTGCCGATAAGCGATTTTCGGCGATGTACTTCAGCCCTTTCATCACATC                                                                                  >  SRR4302537.4041483/1‑94 (MQ=60)
              ATCCGCCTGCGTTTCTCGTCCACGCGGGGAAAGCACACCGTTTTCTGCCGATAAGCGATTTTCGGCGATGTACTTCAGCCCTTTCATCACATCA                                                                                 <  SRR4302537.4299642/94‑1 (MQ=60)
                CCGCCTGCGTTTCTCGTCCACGCGGGGAAAGCACACCGTTTTCTGCCGATAAGCGATTTTCGGCGATGTACTTCAGCCCTTTCATCACATCAGA                                                                               <  SRR4302537.475174/94‑1 (MQ=60)
                CCGCCTGCGTTTCTCGTCCACGCGGGGAAAGCACACCGTTTTCTGCCGATAAGCGATTTTCGGCGATGTACTTCAGCCCTTTCATCACATCAGA                                                                               <  SRR4302537.2992270/94‑1 (MQ=60)
                  GCCTGCGTTTCTCGTCCACGCGGGGAAAGCACACCGTTTTCTGCCGATAAGCGATTTTCGGCGATGTACTTCAGCCCTTTCATCACATCAGAGG                                                                             >  SRR4302537.1507466/1‑94 (MQ=60)
                   CCTGCGTTTCTCGTCCACGCGGGGAAAGCACACCGTTTTCTGCCGATAAGCGATTTTCGGCGATGTACTTCAGCCCTTTCATCACATCAGAGGT                                                                            >  SRR4302537.3776327/1‑94 (MQ=60)
                    CTGCGTTTCTCGTCCACGCGGGGAAAGCACACCGTTTTCTGCCGATAAGCGATTTTCGGCGATGTACTTCAGCCCTTTCATCACATCAGAGGTG                                                                           <  SRR4302537.5931219/94‑1 (MQ=60)
                    CTGCGTTTCTCGTCCACGCGGGGAAAGCACACCGTTTTCTGCCGATAAGCGATTTTCGGCGATGTACTTCAGCCCTTTCATCACATCAGAGGGG                                                                           >  SRR4302537.4866610/1‑94 (MQ=60)
                     TGCGTTTCTCGTCCACGCGGGGAAAGCACACCGTTTTCTGCCGATAAGCGATTTTCGGCGATGTACTTCAGCCCTTTCATCACATCAGAGGTGC                                                                          <  SRR4302537.1314423/94‑1 (MQ=60)
                      GCGTTTCTCGTCCACGCGGGGAAAGCACACCGTTTTCTGCCGATAAGCGATTTTCGGCGATGTACTTCAGCCCTTTCATCACATCAGAGGTGCG                                                                         <  SRR4302537.2880096/94‑1 (MQ=60)
                       CGTTTCTCGTCCACGCGGGGAAAGCACACCGTTTTCTGCCGATAAGCGATTTTCGGCGATGTACTTCAGCCCTTTCATCACATCAGAGGTGCGT                                                                        >  SRR4302537.3641906/1‑94 (MQ=60)
                        GTTTCTCGTCCACGCGGGGAAAGCACACCGTTTTCTGCCGATAAGCGATTTTCGGCGATGTACTTCAGCTCTTTCATCACATCAGAGGTGCGTT                                                                       <  SRR4302537.3971934/94‑1 (MQ=60)
                           TCTCGTCCACGCGGGGAAAGCACACCGTTTTCTGCCGATAAGCGATTTTCGGCGATGTACTTCAGCCCTTTCATCACATCAGAGGTGCGTTGAA                                                                    <  SRR4302537.842586/94‑1 (MQ=60)
                            CTCGTCCACGCGGGGAAAGCACACCGTTTTCTGCCGATAAGCGATTTTCGGCGATGTACTTCAGCCCTTTCATCACATCAGAGGTGCGTTGAAT                                                                   <  SRR4302537.3556629/94‑1 (MQ=60)
                            CTCGTCCACGCGGGGAAAGCACACCGTTTTCTGCCGATAAGCGATTTTCGGCGATGTACTTCAGCCCTTTCATCACATCAGAGGTGCGTTGAAT                                                                   <  SRR4302537.6115909/94‑1 (MQ=60)
                            CTCGTCCACGCGGGGAAAGCACACCGTTTTCTGCCGATAAGCGATTTTCGGCGATGTACTTCAGCCCTTTCATCACATCAGAGGTGCGTTGAAT                                                                   <  SRR4302537.6077330/94‑1 (MQ=60)
                            CTCGTCCACGCGGGGAAAGCACACCGTTTTCTGCCGATAAGCGATTTTCGGCGATGTACTTCAGCCCTTTCATCACATCAGAGGTGCGTTGAAT                                                                   <  SRR4302537.531107/94‑1 (MQ=60)
                             TCGTCCACGCGGGGAAAGCACACCGTTTTCTGCCGATAAGCGATTTTCGGCGATGTACTTCAGCCCTTTCATCACATCAGAGGTGCGTTGAATA                                                                  <  SRR4302537.4474801/94‑1 (MQ=60)
                             TCGTCCACGCGGGGAAAGCACACCGTTTTCTGCCGATAAGCGATTTTCGGCGATGTACTTCAGCCCTTTCATCACATCAGAGGTGCGTTGAATA                                                                  <  SRR4302537.498471/94‑1 (MQ=60)
                              CGTCCACGCGGGGAAAGCACACCGTTTTCTGCCGATAAGCGATTTTCGGCGATGTACTTCAGCCCTTTCATCACATCAGAGGTGCGTTGAATAA                                                                 >  SRR4302537.916974/1‑94 (MQ=60)
                               GTCCACGCGGGGAAAGCACACCGTTTTCTGCCGATAAGCGATTTTCGGCGATGTACTTCAGCCCTTTCATCACATCAGAGGTGCGTTGAATAAA                                                                >  SRR4302537.1610104/1‑94 (MQ=60)
                               GTCCACGCGGGGAAAGCACACCGTTTTCTGCCGATAAGCGATTTTCGGCGATGTACTTCAGCCCTTTCATCACATCAGAGGTGCGTTGAATAAA                                                                >  SRR4302537.5785649/1‑94 (MQ=60)
                                 CCACGCGGGGAAAGCACACCGTTTTCTGCCGATAAGCGATTTTCGGCGATGTACTTCAGCCCTTTCATCACATCAGAGGTGCGTTGAATAAAGC                                                              <  SRR4302537.4123151/94‑1 (MQ=60)
                                 CCACGCGGGGAAAGCACACCGTTTTCTGCCGATAAGCGATTTTCGGCGATGTACTTCAGCCCTTTCATCACATCAGAGGTGCGTTGAATAAAGC                                                              <  SRR4302537.1808435/94‑1 (MQ=60)
                                 CCACGCGGGGAAAGCACACCGTTTTCTGCCGATAAGCGATTTTCGGCGATGTACTTCAGCCCTTTCATCACATCAGAGGTGCGTTGAATAAAGC                                                              <  SRR4302537.6085010/94‑1 (MQ=60)
                                    CGCGGGGAAAGCACACCGTTTTCTGCCGATAAGCGATTTTCGGCGATGTACTTCAGCCCTTTCATCACATCAGAGGTGCGTTGAATAAAGCGCT                                                           <  SRR4302537.5116905/94‑1 (MQ=60)
                                    CGCGGGGAAAGCACACCGTTTTCTGCCGATAAGCGATTTTCGGCGATGTACTTCAGCCCTTTCATCACATCAGAGGTGCGTTGAATAAAGCGCT                                                           <  SRR4302537.4797269/94‑1 (MQ=60)
                                     GCGGGGAAAGCACACCGTTTTCTGCCGATAAGCGATTTTCGGCGATGTACTTCAGCCCTTTCATCACATCAGAGGTGCGTTGAATAAAGCGCTG                                                          >  SRR4302537.2842638/1‑94 (MQ=60)
                                      CGGGGAAAGCACACCGTTTTCTGCCGATAAGCGATTTTCGGCGATGTACTTCAGCCCTTTCATCACATCAGAGGTGCGTTGAATAAAGCGCTGA                                                         >  SRR4302537.2049941/1‑94 (MQ=60)
                                       GGGGAAAGCACACCGTTTTCTGCCGATAAGCGATTTTCGGCGATGTACTTCAGCCCTTTCATCACATCAGAGGTGCGTTGAATAAAGCGCTGAG                                                        >  SRR4302537.6043068/1‑94 (MQ=60)
                                          GAAAGCACACCGTTTTCTGCCGATAAGCGATTTTCGGCGATGTACTTCAGCCCTTTCATCACATCAGAGGTGCGTTGAATAAAGCGCTGAGCCT                                                     >  SRR4302537.1484281/1‑94 (MQ=60)
                                          GAAAGCACACCGTTTTCTGCCGATAAGCGATTTTCGGCGATGTACTTCAGCCCTTTCATCACATCAGAGGTGCGTTGAATAAAGCGCTGAGCCT                                                     >  SRR4302537.3694278/1‑94 (MQ=60)
                                           AAAGCACACCGTTTTCTGCCGATAAGCGATTTTCGGCGATGTACTTCAGCCCTTTCATCACATCAGAGGTGCGTTGAATAAAGCGCTGAGCCTG                                                    <  SRR4302537.5768120/94‑1 (MQ=60)
                                            AAGCACACCGTTTTCTGCCGATAAGCGATTTTCGGCGATGTACTTCAGCCCTTTCATCACATCAGAGGTGCGTTGAATAAAGCGCTGAGCCTGA                                                   >  SRR4302537.3402807/1‑94 (MQ=60)
                                             AGCACACCGTTTTCTGCCGATAAGCGATTTTCGGCGATGTACTTCAGCCCTTTCATCACATCAGAGGTGCGTTGAATAAAGCGCTGAGCCTGAT                                                  >  SRR4302537.1973291/1‑94 (MQ=60)
                                             AGCACACCGTTTTCTGCCGATAAGCGATTTTCGGCGATGTACTTCAGCCCTTTCATCACATCAGAGGTGCGTTGAATAAAGCGCTGAGCCTGAT                                                  <  SRR4302537.4455404/94‑1 (MQ=60)
                                             AGCACACCGTTTTCTGCCGATAAGCGATTTTCGGCGATGTACTTCAGCCCTTTCACCACATCAGAGGTGCGTTGAATAAAGCGCTGAGCCTGAT                                                  <  SRR4302537.6471258/94‑1 (MQ=60)
                                              GCACACCGTTTTCTGCCGATAAGCGATTTTCGGCGATGTACTTCAGCCCTTTCATCACATCAGAGGTGCGTTGAATAAAGCGCTGAGCCTGATC                                                 <  SRR4302537.5929504/94‑1 (MQ=60)
                                              GCACACCGTTTTCTGCCGATAAGCGATTTTCGGCGATGTACTTCAGCCCTTTCATCACATCAGAGGTGCGTTGAATAAAGCGCTGAGCCTGATC                                                 <  SRR4302537.840391/94‑1 (MQ=60)
                                              GCACACCGTTTTCTGCCGATAAGCGATTTTCGGCGATGTACTTCAGCCCTTTCATCACATCAGAGGTGCGTTGAATAAAGCGCTGAGCCTGATC                                                 <  SRR4302537.4703931/94‑1 (MQ=60)
                                              GCACACCGTTTTCTGCAGATAAGCGATTTTCGGCGATGTACTTCAGCCCTTTCATCACATCAGAGGTGCGTTGAATAAAGCGCTGAGCCTGATC                                                 <  SRR4302537.6095362/94‑1 (MQ=60)
                                               CACACCGTTTTCTGCCGATAAGCGATTTTCGGCGATGTACTTCAGCCCTTTCATCACATCAGAGGTGCGTTGAATAAAGCGCTGAGCCTGATCG                                                >  SRR4302537.6480081/1‑94 (MQ=60)
                                               CACACCGTTTTCTGCCGATAAGCGATTTTCGGCGATGTACTTCAGCCCTTTCATCACATCAGAGGTGCGTTGAATAAAGCGCTGAGCCTGATCG                                                <  SRR4302537.4643161/94‑1 (MQ=60)
                                                ACACCGTTTTCTGCCGATAAGCGATTTTCGGCGATGTACTTCAGCCCTTTCATCACATCAGAGGTGCGTTGAATAAAGCGCTGAGCCTGATCGG                                               <  SRR4302537.6411030/94‑1 (MQ=60)
                                                 CACCGTTTTCTGCCGATAAGCGATTTTCGGCGATGTACTTCAGCTCTTTCATCACATCAGAGGTGCGTTGAATAAAGCGCTGAGCCTGATCGGA                                              <  SRR4302537.1525961/94‑1 (MQ=60)
                                                   CCGTTTTCTGCCGATAAGCGATTTTCGGCGATGTACTTCAGCCCTTTCATCACATCAGAGGTGCGTTGAATAAAGCGCTGAGCCTGATCGGAAC                                            <  SRR4302537.2947495/94‑1 (MQ=60)
                                                    CGTTTTCTGCCGATAAGCGATTTTCGGCGATGTACTTCAGCCCTTTCATCACATCAGAGGTGCGTTGAATAAAGCGCTGAGCCTGATCGGAACT                                           <  SRR4302537.629321/94‑1 (MQ=60)
                                                     GTTTTCTGCCGATAAGCGATTTTCGGCGATGTACTTCAGCCCTTTCATCACATCAGAGGTGCGTTGAATAAAGCGCTGAGCCTGATCGGAACTC                                          >  SRR4302537.3228547/1‑94 (MQ=60)
                                                      TTTTCTGCCGATAAGCGATTTTCGGCGATGTACTTCAGCCCTTTCATCACATCAGAGGTGCGTTGAATAAAGCGCTGAGCCTGATCGGAACTCA                                         <  SRR4302537.2589206/94‑1 (MQ=60)
                                                      TTTTCTGCCGATAAGCGATTTTCGGCGATGTACTTCAGCCCTTTCATCACATCAGAGGTGCGTTGAATAAAGCGCTGAGCCTGATCGGAACTCA                                         >  SRR4302537.2151544/1‑94 (MQ=60)
                                                      TTTTCTGCCGATAAGCGATTTTCGGCGATGTACTTCAGCCCTTTCATCACATCAGAGGTGCGTTGAATAAAGCGCTGAGCCTGATCGGAACTCA                                         <  SRR4302537.2494231/94‑1 (MQ=60)
                                                       TTTCTGCCGATAAGCGATTTTCGGCGATGTACTTCAGCCCTTTCATCACATCAGAGGTGCGTTGAATAAAGCGCTGAGCCTGATCGGAACTCAG                                        <  SRR4302537.5504394/94‑1 (MQ=60)
                                                          CTGCCGATAAGCGATTTTCGGCGATGTACTTCAGCCCTTTCATCACATCAGAGGTGCGTTGAATAAAGCGCTGAGCCTGATCGGAACTCAGATT                                     <  SRR4302537.829729/94‑1 (MQ=60)
                                                            GCCGATAAGCGATTTTCGGCGATGTACTTCAGCCCTTTCATCACATCAGAGGTGCGTTGAATAAAGCGCTGAGCCTGATCGGAACTCAGATTAA                                   >  SRR4302537.2276337/1‑94 (MQ=60)
                                                              CGATAAGCGATTTTCGGCGATGTACTTCAGCCCTTTCATCACATCAGAGGTGCGTTGAATAAAGCGCTGAGCCTGATCGGAACTCAGATTAAAT                                 >  SRR4302537.5500956/1‑94 (MQ=60)
                                                              CGATAAGCGATTTTCGGCGATGTACTTCAGCCCTTTCATCACATCAGAGGTGCGTTGAATAAAGCGCTGAGCCTGATCGGAACTCAGATTAAAT                                 <  SRR4302537.5553607/94‑1 (MQ=60)
                                                              CGATAAGCGATTTTCGGCGATGTACTTCAGCCCTTTCATCACATCAGAGGTGCGTTGAATAAAGCGCTGAGCCTGATCGGAACTCAGATTAAAT                                 >  SRR4302537.955039/1‑94 (MQ=60)
                                                              CGATAAGCGATTTTCGGCGATGTACTTCAGCCCTTTCATCACATCAGAGGTGCGTTGAATAAAGCGCTGAGCCTGATCGGAACTCAGATTAAAT                                 >  SRR4302537.2712086/1‑94 (MQ=60)
                                                              CGATAAGCGATTTTCGGCGATGTACTTCAGCCCTTTCATCACATCAGAGGTGCGTTGAATAAAGCGCTGAGCCTGATCGGAACTCAGATTAAAT                                 <  SRR4302537.3815486/94‑1 (MQ=60)
                                                               GATAAGCGATTTTCGGCGATGTACTTCAGCCCTTTCATCACATCAGAGGTGCGTTGAATAAAGCGCTGAGCCTGATCGGAACTCAGATTAAATT                                <  SRR4302537.3387306/94‑1 (MQ=60)
                                                               GATAAGCGATTTTCGGCGATGTACTTCAGCCCTTTCATCACATCAGAGGTGCGTTGAATAAAGCGCTGAGCCTGATCGGAACTCAGATTAAATT                                <  SRR4302537.876440/94‑1 (MQ=60)
                                                               GATAAGCGATTTTCGGCGATGTACTTCAGCCCTTTCATCACATCAGAGGTGCGTTGAATAAAGCGCTGAGCCTGATCGGAACTCAGATTAAATT                                >  SRR4302537.2604658/1‑94 (MQ=60)
                                                               GATAAGCGATTTTCGGCGATGTACTTCAGCCCTTTCATCACATCAGAGGTGCGTTGAATAAAGAGCTGAGCCTGATCGGAACTCAGATTAAATT                                >  SRR4302537.3603811/1‑94 (MQ=60)
                                                                ATAAGCGATTTTCGGCGATGTACTTCAGCCCTTTCATCACATCAGAGGTGCGTTGAATAAAGCGCTGAGCCTGATCGGAACTCAGATTAAATTC                               >  SRR4302537.1733509/1‑94 (MQ=60)
                                                                 TAAGCGATTTTCGGCGATGTACTTCAGCCCTTTCATCACATCAGAGGTGCGTTGAATAAAGCGCTGAGCCTGATCGGAACTCAGATTAAATTCC                              <  SRR4302537.4622603/94‑1 (MQ=60)
                                                                 TAAGCGATTTTCGGCGATGTACTTCAGCCCTTTCATCACATCAGAGGTGCGTTGAAGAAAGCGCTGAGCCTGATCGGAACTCAGATTAAATTCC                              <  SRR4302537.4523753/94‑1 (MQ=60)
                                                                  AAGCGATTTTCGGCGATGTACTTCAGCCCTTTCATCACATCAGAGGTGCGTTGAATAAAGCGCTGAGCCTGATCGGAACTCAGATTAAATTCCT                             >  SRR4302537.4750643/1‑94 (MQ=60)
                                                                    GCGATTTTCGGCGATGTACTTCAGCCCTTTCATCACATCAGAGGTGCGTTGAATAAAGCGCTGAGCCTGATCGGAACTCAGATTAAATTCCTGA                           >  SRR4302537.5894590/1‑94 (MQ=60)
                                                                    GCGATTTTCGGCGATGTACTTCAGCCCTTTCATCACATCAGAGGTGCGTTGAATAAAGCGCTGAGCCTGATCGGAACTCAGATTAAATTCCTGA                           >  SRR4302537.2078093/1‑94 (MQ=60)
                                                                     CGATTTTCGGCGATGTACTTCAGCCCTTTCATCACATCAGAGGTGCGTTGAATAAAGCGCTGAGCCTGATCGGAACTCAGATTAAATTCCTGAC                          <  SRR4302537.5272871/94‑1 (MQ=60)
                                                                     CGATTTTCGGCGATGTACTTCAGCCCTTTCATCACATCAGAGGTGCGTTGAATAAAGCGCTGAGCCTGATCGGAACTCAGATTAAATTCCTGAC                          <  SRR4302537.893036/94‑1 (MQ=60)
                                                                      GATTTTCGGCGATGTACTTCAGCCCTTTCATCACATCAGAGGTGCGTTGAATAAAGCGCTGAGCCTGATCGGAACTCAGATTAAATTCCTGACG                         >  SRR4302537.5227464/1‑94 (MQ=60)
                                                                      GATTTTCGGCGATGTACTTCAGCCCTTTCATCACATCAGAGGTGCGTTGAATAAAGCGCTGAGCCTGATCGGAACTCAGATTAAATTCCTGACG                         >  SRR4302537.1565816/1‑94 (MQ=60)
                                                                      GATTTTCGGCGATGTACTTCAGCCCTTTCATCACATCAGAGGTGCGTTGAATAAAGCGCTGAGCCTGATCGGAACTCAGATTAAATTCCTGACG                         <  SRR4302537.955013/94‑1 (MQ=60)
                                                                      GATTTTCGGCGATGTACTTCAGCCCTTTCATCACATCAGAGGTGCGTTGAATAAAGCGCTGAGCCTGATCGGAACTCAGATTAAATTCCTGACG                         <  SRR4302537.4828356/94‑1 (MQ=60)
                                                                           TCGGCGATGTACTTCAGCCCTTTCATCACATCAGAGGTGCGTTGAATAAAGCGCTGAGCCTGATCGGAACTCAGATTAAATTCCTGACGAATTT                    <  SRR4302537.1873498/94‑1 (MQ=60)
                                                                             GGCGATGTACTTCAGCCCTTTCATCACATCAGAGGTGCGTTGAATAAAGCGCTGAGCCTGATCGGAACTCAGATTAAATTCCTGACGAATTTCC                  <  SRR4302537.3691473/94‑1 (MQ=60)
                                                                              GCGATGTACTTCAGCCCTTTCATCACATCAGAGGTGCGTTGAATAAAGCGCTGAGCCTGATCGGAACTCAGATTAAATTCCTGACGAATTTCCG                 <  SRR4302537.3923882/94‑1 (MQ=60)
                                                                              GCGATGTACTTCAGCCCTTTCATCACATCAGAGGTGCGTTGAATAAAGCGCTGAGCCTGATCGGAACTCAGATTAAATTCCTGACGAATTTCCG                 >  SRR4302537.3516696/1‑94 (MQ=60)
                                                                               CGATGTACTTCAGCCCTTTCATCACATCAGAGGTGCGTTGAATAAAGCGCTGAGCCTGATCGGAACTCAGATTAAATTCCTGACGAATTTCCGA                >  SRR4302537.5975783/1‑94 (MQ=60)
                                                                                GATGTACTTCAGCCCTTTCATCACATCAGAGGTGCGTTGAATAAAGCGCTGAGCCTGATCGGAACTCAGATTAAATTCCTGACGAATTTCCGAT               <  SRR4302537.6081063/94‑1 (MQ=60)
                                                                                GATGTACTTCAGCCCTTTCATCACATCAGAGGTGCGTTGAATAAAGCGCTGAGCCTGATCGGAACTCAGATTAAATTCCTGACGAATTTCCGAT               <  SRR4302537.2187216/94‑1 (MQ=60)
                                                                                 ATGTACTTCAGCCCTTTCATCACATCAGAGGTGCGTTGAATAAAGCGCTGAGCCTGATCGGAACTCAGATTAAATTCCTGACGAATTTCCGATT              >  SRR4302537.5855299/1‑94 (MQ=60)
                                                                                 ATGTACTTCAGCCCTTTCATCACATCAGAGGTGCGTTGAATAAAGCGCTGAGCCTGATCGGAACTCAGATTAAATTCCTGACGAATTTCCGATT              >  SRR4302537.3739985/1‑94 (MQ=60)
                                                                                 ATGTACTTCAGCCCTTTCATCACATCAGAGGTGCGTTGAATAAAGCGCTGAGCCTGATCGGAACTCAGATTAAATTCCTGACGAATTTCCGATT              >  SRR4302537.2209710/1‑94 (MQ=60)
                                                                                 ATGTACTTCAGCCCTTTCATCACATCAGAGGTGCGTTGAATAAAGCGCTGAGCCTGATCGGAACTCAGATTAAATTCCTGACGAATTTCCGATT              <  SRR4302537.2102588/94‑1 (MQ=60)
                                                                                 ATGTACTTCAGCCCTTTCATCACATCAGAGGTGCGTTGAATAAAGCGCTGAGCCTGATCGGAACTCAGATTAAATTCCTGACGAATTTCCGATT              >  SRR4302537.684892/1‑94 (MQ=60)
                                                                                  TGTACTTCAGCCCTTTCATCACATCAGAGGTGCGTTGAATAAAGCGCTGAGCCTGATCGGAACTCAGATTAAATTCCTGACGAATTTCCGATTC             >  SRR4302537.3556406/1‑94 (MQ=60)
                                                                                   GTACTTCAGCCCTTTCATCACATCAGAGGTGCGTTGAATAAAGCGCTGAGCCTGATCGGAACTCAGATTAAATTCCTGACGAATTTCCGATTCT            <  SRR4302537.2927467/94‑1 (MQ=60)
                                                                                    TACTTCAGCCCTTTCATCACATCAGAGGTGCGTTGAATAAAGCGCTGAGCCTGATCGGAACTCAGATTAAATTCCTGACGAATTTCCGATTCTC           <  SRR4302537.1685702/94‑1 (MQ=60)
                                                                                     ACTTCAGCCCTTTCATCACATCAGAGGTGCGTTGAATAAAGCGCTGAGCCTGATCGGAACTCAGATTAAATTCCTGACGAATTTCCGATTCTCG          >  SRR4302537.4831199/1‑94 (MQ=60)
                                                                                     ACTTCAGCCCTTTCATCACATCAGAGGTGCGTTGAATAAAGCGCTGAGCCTGATCGGAACTCAGATTAAATTCCTGACGAATTTCCGATTCTCG          <  SRR4302537.3856457/94‑1 (MQ=60)
                                                                                     ACTTCAGCCCTTTCATCACATCAGAGGTGCGTTGAATAAAGCGCTGAGCCTGATCGGAACTCAGATTAAATTCCTGACGAATTTCCGATTCTCG          <  SRR4302537.4103661/94‑1 (MQ=60)
                                                                                      CTTCAGCCCTTTCATCACATCAGAGGTGCGTTGAATAAAGCGCTGAGCCTGATCGGAACTCAGATTAAATTCCTGACGAATTTCCGATTCTCGC         >  SRR4302537.3728990/1‑94 (MQ=60)
                                                                                      CTTCAGCCCTTTCATCACATCAGAGGTGCGTTGAATAAAGCGCTGAGCCTGATCGGAACTCAGATTAAATTCCTGACGAATTTCCGATTCTCGC         >  SRR4302537.5008058/1‑94 (MQ=60)
                                                                                       TTCAGCCCTTTCATCACATCAGAGGTGCGTTGAATAAAGCGCTGAGCCTGATCGGAACTCAGATTAAATTCCTGACGAATTTCCGATTCTCGCT        <  SRR4302537.4763570/94‑1 (MQ=60)
                                                                                       TTCAGCCCTTTCATCACATCAGAGGTGCGTTGAATAAAGCGCTGAGCCTGATCGGAACTCAGATTAAATTCCTGACGAATTTCCGATTCTCGCT        >  SRR4302537.185256/1‑94 (MQ=60)
                                                                                        TCAGCCCTTTCATCACATCAGAGGTGCGTTGAATAAAGCGCTGAGCCTGATCGGAACTCAGATTAAATTCCTGACGAATTTCCGATTCTCGCTG       <  SRR4302537.1350039/94‑1 (MQ=60)
                                                                                         CAGCTCTTTCATCACATCAGAGGTGCGTTGAATAAAGCGCTGAGCCTGATCGGAACTCAGATTAAATTCCTGACGAATTTCCGATTCTCGCTGA      >  SRR4302537.2774671/1‑94 (MQ=60)
                                                                                          AGCCCTTTCATCACATCAGAGGTGCGTTGAATAAAGCGCTGAGCCTGATCGGAACTCAGATTAAATTCCTGACGAATTTCCGATTCTCGCTGAT     >  SRR4302537.3927075/1‑94 (MQ=60)
                                                                                          AGCCCTTTCATCACATCAGAGGTGCGTTGAATAAAGCGCTGAGCCTGATCGGAACTCAGATTAAATTCCTGACGAATTTCCGATTCTCGCTGAT     >  SRR4302537.6233652/1‑94 (MQ=60)
                                                                                          AGCCCTTTCATCACATCAGAGGTGCGTTGAATAAAGCGCTGAGCCTGATCGGAACTCAGATTAAATTCCTGACGAATTTCCGATTCTCGCTGAT     <  SRR4302537.6175587/94‑1 (MQ=60)
                                                                                             CCTTTCATCACATCAGAGGTGCGTTGAATAAAGCGCTGAGCCTGATCGGAACTCAGATTAAATTCCTGACGAATTTCCGATTCTCGCTGATGTA  <  SRR4302537.4493848/94‑1 (MQ=60)
                                                                                             CCTTTCATCACATCAGAGGTGCGTTGAATAAAGCGCTGAGCCTGATCGGAACTCAGATTAAATTCCTGACGAATTTCCGATTCTCGCTGATGTA  >  SRR4302537.45307/1‑94 (MQ=60)
                                                                                             CCTTTCATCACATCAGAGGTGCGTTGAATAAAGCGCTGAGCCTGATCGGAACTCAGATTAAATTCCTGACGAATTTCCGATTCTCGCTGATGTA  <  SRR4302537.1315428/94‑1 (MQ=60)
                                                                                             |                                                                                             
TCAAACGCAGGCACATCCGCCTGCGTTTCTCGTCCACGCGGGGAAAGCACACCGTTTTCTGCCGATAAGCGATTTTCGGCGATGTACTTCAGCTCTTTCATCACATCAGAGGTGCGTTGAATAAAGCGCTGAGCCTGATCGGAACTCAGATTAAATTCCTGACGAATTTCCGATTCTCGCTGATGTA  >  NC_000913/2317589‑2317775

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 18 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑