Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I2 R1 882 254.4 12957588 98.0% 12698436 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,633,072 T→C V274A (GTG→GCG)  xseA → exonuclease VII large subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,633,0720TC100.0% 707.6 / NA 205V274A (GTG→GCG) xseAexonuclease VII large subunit
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (100/105);  total (100/105)

Only 100 of 217 total aligned reads displayed.

TTGCCGATTTTGTTGCCGATCTGCGTGCGCCAACGCCGTCTGCCGCCGCTGAAGTAGTGAGCCGTAATCAGCAAGAGTTACTGCGCCAGGTGCAATCGACCCGTCAACGGCTGGAGATGGCGATGGATTATTATCTCGCCAACCGCACACGTCGCTTTACGCAAATTCATCACCGATTAC  >  NC_000913/2632982‑2633161
                                                                                          |                                                                                         
  gCCGATTTTGTTGCCGATCTGCGTGCGCCAACGCCGTCTGCCGCCGCTGAAGTAGTGAGCCGTAATCAGCAAGAGTTACTGCGCCAGGCGCaa                                                                                       <  2:3573441/93‑1 (MQ=255)
  gCCGATTTTGTTGCCGATCTGCGTGCGCCAACGCCGTCTGCCGCCGCTGAAGTAGTGAGCCGTAATCAGCAAGAGTTACTGCGCCAGGCGCaa                                                                                       <  2:2720363/93‑1 (MQ=255)
   ccGATTTTGTTGCCGATCTGCGTGCGCCAACGCCGTCTGCCGCCGCTGAAGTAGTGAGCCGTAATCAGCAAGAGTTACTGCGCCAGGCGCAAt                                                                                      >  2:4342138/1‑93 (MQ=255)
   ccGATTTTGTTGCCGATCTGCGTGCGCCAACGCCGTCTGCCGCCGCTGAAGTAGTGAGCCGTAATCAGCAAGAGTTACTGCGCCAGGCGCAAt                                                                                      <  2:4596495/93‑1 (MQ=255)
    cGATTTTGTTGCCGATCTGCGTGCGCCAACGCCGTCTGCCGCCGCTGAAGTAGTGAGCCGTAATCAGCAAGAGTTACTGCGCCAGGCGCAATc                                                                                     <  2:1562734/93‑1 (MQ=255)
    cGATTTTGTTGCCGATCTGCGTGCGCCAACGCCGTCTGCCGCCGCTGAAGTAGTGAGCCGTAATCAGCAAGAGTTACTGCGCCAGGCGCAATc                                                                                     <  1:512184/93‑1 (MQ=255)
     gATTTTGTTGCCGATCTGCGTGCGCCAACGCCGTCTGCCGCCGCTGAAGTAGTGAGCCGTAATCAGCAAGAGTTACTGCGCCAGGCGCAATCg                                                                                    >  1:653191/1‑93 (MQ=255)
     gATTTTGTTGCCGATCTGCGTGCGCCAACGCCGTCTGCCGCCGCTGAAGTAGTGAGCCGTAATCAGCAAAAGTTACTGCGCCCGGCGCAATCg                                                                                    >  2:5311525/1‑93 (MQ=255)
       ttttGTTGCCGATCTGCGTGCGCCAACGCCGTCTGCCGCCGCTGAAGTAGTGAGCCGTAATCAGCAAGAGTTACTGCGCCAGGCGCAATCGAc                                                                                  <  2:303205/93‑1 (MQ=255)
       ttttGTTGCCGATCTGCGTGCGCCAACGCCGTCTGCCGCCGCTGAAGTAGTGAGCCGTAATCAGCAAGAGTTACTGCGCCAGGCGCAATCGAc                                                                                  >  1:4309034/1‑93 (MQ=255)
        tttGTTGCCGATCTGCGTGCGCCAACGCCGTCTGCCGCCGCTGAAGTAGTGAGCCGTAATCAGCAAGAGTTACTGCGCCAGGCGCAATCGAcc                                                                                 >  1:3111764/1‑93 (MQ=255)
         ttgttgCCGATCTGCGTGCGCCAACGCCGTCTGCCGCCGCTGAAGTAGTGAGCCGTAATCAGCAAGAGTTACTGCGCCAGGCGCAATCGAccc                                                                                >  1:3790141/1‑93 (MQ=255)
            ttgCCGATCTGCGTGCGCCAACGCCGTCTGCCGCCGCTGAAGTAGTGAGCCGTAATCAGCAAGAGTTACTGCGCCAGGCGCAATCGACCAGTc                                                                             >  2:3974011/1‑93 (MQ=255)
             tgCCGATCTGCGTGCGCCAACGCCGTCTGCCGCCGCTGAAGTAGTGAGCCGTAATCAGCAAGAGTTACTGCGCCAGGCGCAATCGACCCGTCa                                                                            <  2:4320428/93‑1 (MQ=255)
              gCCGATCTGCGTGCGCCAACGCCGTCTGCCGCCGCTGAAGTAGTGAGCCGTAATCAGCAAGAGTTACTGCGCCAGGCGCAATCGACCCGTCaa                                                                           >  2:240469/1‑93 (MQ=255)
              gCCGATCTGCGTGCGCCAACGCCGTCTGCCGCCGCTGAAGTAGTGAGCCGTAATCAGCAAGAGTTACTGCGCCAGGCGCAATCGACCCGTCaa                                                                           <  1:1506088/93‑1 (MQ=255)
              gCCGATCTGCGTGCGCCAACGCCGTCTGCCGCCGCTGAAGTAGTGAGCCGTAATCAGCAAGAGTTACTGCGCCAGGCGCAATCGACCCGTCaa                                                                           >  1:5578233/1‑93 (MQ=255)
              gCCGATCTGCGTGCGCCAACGCCGTCTGCCGCCGCTGAAGTAGTGAGCCGTAATCAGCAAGAGTTACTGCGCCAGGCGCAATCGACCCGTCaa                                                                           <  2:3842873/93‑1 (MQ=255)
              gCCGATCTGCGGGCCCCAACGCCGTCTGCCGCCGCTGAAGTAGTGAGCCGTAATCAGCAAGAGTTACTGCGCCAGGCGCAATCGACCCGTCaa                                                                           <  1:4252653/93‑1 (MQ=255)
               ccGATCTGCGTGCGCCAACGCCGTCTGCCGCCGCTGAAGTAGTGAGCCGTAATCAGCAAGAGTTACTGCGCCAGGCGCAATCGACCCGTCAAc                                                                          <  2:2670999/93‑1 (MQ=255)
               ccGATCTGCGTGCGCCAACGCCGTCTGCCGCCGCTGAAGTAGTGAGCCGTAATCAGCAAGAGTTACTGCGCCAGGCGCAATCGACCCGTCAAc                                                                          <  1:4507854/93‑1 (MQ=255)
               ccGATCTGCGTGCGCCAACGCCGTCTGCCGCCGCTGAAGTAGTGAGCCGTAATCAGCAAGAGTTACTGCGCCAGGCGCAATCGACCCGTCAAc                                                                          <  1:6259409/93‑1 (MQ=255)
                cGATCTGCGTGCGCCACCGCCGTCTGCCGCCGCTGAAGTAGTGAGCCGTAATCAGCAAGAGTTACTGCGCCAGGCGCAATCGACCCGTCAACg                                                                         <  1:3618978/93‑1 (MQ=255)
                 gATCTGCGTGCGCCAACGCCGTCTGCCGCCGCTGAAGTAGTGAGCCGTAATCAGCAAGAGTTACTGCGCCAGGCGCAATCGACCCGTCAACgg                                                                        <  1:3175841/93‑1 (MQ=255)
                 gATCTGCGTGCGCCAACGCCGTCTGCCGCCGCTGAAGTAGTGAGCCGTAATCAGCAAGAGTTACTGCGCCAGGCGCAATCGACCCGTCAACgg                                                                        >  1:757125/1‑93 (MQ=255)
                  aTCTGCGTGCGCCACCGCCGTCTGCCGCCGCTGAAGTAGTGAGCCGTAATCAGCAAGAGTTACTGCGCCAGGCGCAATCGACCCGTCAACGGc                                                                       <  1:2119723/93‑1 (MQ=255)
                  aTCTGCGTGCGCCAACGCCGTCTGCCGCCGCTGAAGTAGTGAGCCGTAATCAGCAAGAGTTACTGCGCCAGGCGCAATCGACCCGTCAACGGc                                                                       >  2:2794683/1‑93 (MQ=255)
                  aTCTGCGTGCGCCAACGCCGTCTGCCGCCGCTGAAGTAGTGAGCCGTAATCAGCAAGAGTTACTGCGCCAGGCGCAATCGACCCGTCAACGGc                                                                       <  2:4146806/93‑1 (MQ=255)
                  aTCTGCGTGCGCCAACGCCGTCTGCCGCCGCTGAAGTAGTGAGCCGTAATCAGCAAGAGTTACTGCGCCAGGCGCAATCGACCCGTCAACGGc                                                                       <  2:2323755/93‑1 (MQ=255)
                  aTCTGCGTGCGCCAACGCCGTCTGCCGCCGCTGAAGTAGTGAGCCGTAATCAGCAAGAGTTACTGCGCCAGGCGCAATCGACCCGTCAACGGc                                                                       >  1:2534248/1‑93 (MQ=255)
                   tCTGCGTGCGCCAACGCCGTCTGCCGCCGCTGAAGTAGTGAGCCGTAATCAGCAAGAGTTACTGCGCCAGGCGCAATCGACCCGTCAACGGCt                                                                      >  1:3764164/1‑93 (MQ=255)
                     tgcgtgcgCCAACGCCGTCTGCCGCCGCTGAAGTAGTGAGCCGTAATCAGCAAGAGTTACTGCGCCAGGCGCAATCGACCCGTCAACGGCTgg                                                                    >  1:5463375/1‑93 (MQ=255)
                     tgcgtgcgCCAACGCCGTCTGCCGCCGCTGAAGTAGTGAGCCGTAATCAGCAAGAGTTACTGCGCCAGGCGCAATCGACCCGTCAACGGCTgg                                                                    >  1:348923/1‑93 (MQ=255)
                        gtgccCCAACCCCGTCTGCCGCCGCTGAAGTAGTGAGCCGTAATCAGCAAGAGTTACTGCGCCAGGCGCAATCGACCCGTCAACGGCTGgaga                                                                 <  1:3413076/93‑1 (MQ=255)
                         tgcgCCAACGCCGTCTGCCGCCGCTGAAGTAGTGAGCCGTAATCAGCAAGAGTTACTGCGCCAGGCGCAATCGACCCGTCAACGGCTGGAGAt                                                                <  2:5928450/93‑1 (MQ=255)
                         tgcgCCAACGCCGTCTGCCGCCGCTGAAGTAGTGAGCCGTAATCAGCAAGAGTTACTGCGCCAGGCGCAATCGACCCGTCAACGGCTGGAGAt                                                                <  1:4976964/93‑1 (MQ=255)
                          gcgcCAACGCCGTCTGCCGCCGCTGAAGTAGTGAGCCGTAATCAGCAAGAGTTACTGCGCCAGGCGCAATCGACCCGTCAACGGCTGGAGATg                                                               <  2:3374374/93‑1 (MQ=255)
                           cgcCAACGCCGTCTGCCGCCGCTGAAGTAGTGAGCCGTAATCAGCAAGAGTTACTGCGCCAGGCGCAATCGACCCGTCAACGGCTGGAGATgg                                                              <  1:5357506/93‑1 (MQ=255)
                            gcCAACGCCGTCTGCCGCCGCTGAAGTAGTGAGCCGTAATCAGCAAGAGTTACTGCGCCAGGCGCAATCGACCCGTCAACGGCTGGAGATGGc                                                             <  2:3098829/93‑1 (MQ=255)
                             ccAGCGCTGTCTGCCGCCGCTGAAGTAGTGAGCCGTAATCAGCAAGAGTTACTGCGCCAGGCGCAATCGACCCGTCAACGGCTGGAGATGGCg                                                            <  1:2603506/93‑1 (MQ=255)
                             ccAACGCCGTCTGCCGCCGCTGAAGTAGTGAGCCGTAATCAGCAAGAGTTACTGCGCCAGGCGCAATCGACCCGTCAACGGCTGGAGATGGCg                                                            >  2:4642743/1‑93 (MQ=255)
                               aaCGCCGTCTGCCGCCGCTGAAGTAGTGAGCCGTAATCAGCAAGAGTTACTGCGCCAGGCGCAATCGACCCGTCAACGGCTGGAGATGGCGAt                                                          >  1:635229/1‑93 (MQ=255)
                               aaCGCCGTCTGCCGCCGCTGAAGTAGTGAGCCGTAATCAGCAAGAGTTACTGCGCCAGGCGCAATCGACCCGTCAACGGCTGGAGATGGCGAt                                                          <  2:3654944/93‑1 (MQ=255)
                                aagCCGTCTGCCGCCGCTGAAGTAGTGAGCCGTAATCAGCAAGAGTTACTGCGCCAGGCGCAATCGACCCGTCAACGGCTGGAGATGGCGATg                                                         <  1:1201741/91‑1 (MQ=255)
                                 cGCCTTCTGCCGCCGCTGAAGTAGTGAGCCGTAATCAGCAAGAGTTACTGCGCCAGGCGCAATCGACCCGTCAACGGCTGGAGATGGCGATgg                                                        <  1:1777118/93‑1 (MQ=255)
                                 cGCCGTCTGCCGCCGCTGAAGTAGTGAGCCGTAATCAGCAAGAGCTACTGCGCCAGGCGCAATCGACCCGTCAACGGCTGGAGATGGCGATgg                                                        >  1:5435962/1‑93 (MQ=255)
                                  gCCGTCTGCCGCCGCTGAAGTAGTGAGCCGTAATCAGCAAGAGTTACTGCGCCAGGCGCAATCGACCCGTCAACGGCTGGAGATGGCGATGGa                                                       >  1:2992842/1‑93 (MQ=255)
                                    cGTCTGCCGCCGCTGAAGTAGTGAGCCGTAATCAGCAAGAGTTACTGCGCCAGGCGCAATCGACCCGTCAACGGCTGGAGATGGCGATGGatt                                                     <  1:1638184/93‑1 (MQ=255)
                                     gTCTGCCGCCGCTGAAGTAGTGAGCCGTAATCAGCAAGAGTTACTGCGCCAGGCGCAATCGACCCGTCAACGGCTGGAGATGGCGATGGatta                                                    >  2:6003479/1‑93 (MQ=255)
                                     gTCTGCCGCCGCTGAAGTAGTGAGCCGTAATCAGCAAGAGTTACTGCGCCAGGCGCAATCGACCCGTCAACGGCTGGAGATGGCGATGGatta                                                    >  2:6425193/1‑93 (MQ=255)
                                        tGCCGCCGCTGAAGTAGTGAGCCGTAATCAGCAAGAGTTACTGCGCCAGGCGCAATCGACCCGTCAACGGCTGGAGATGGCGATGGGttatta                                                 >  1:1587323/1‑93 (MQ=255)
                                        tGCCGCCGCTGAAGTAGTGAGCCGTAATCAGCAAGAGTTACTGCGCCAGGCGCAATCGACCCGTCAACGGCTGGAGATGGCGATGGAttatta                                                 >  1:1479832/1‑93 (MQ=255)
                                          ccgccgCTGAAGTAGTGAGCCGTAATCAGCAAGAGTTACTGCGCCAGGCGCAATCGACCCGTCAACGGCTGGAGATGGCGATGGATTATTAtc                                               >  1:2007283/1‑93 (MQ=255)
                                          ccgccgCTGAAGTAGTGAGCCGTAATCAGCAAGAGTTACTGCGCCAGGCGCAATCGACCCGTCAACGGCTGGAGATGGCGATGGATTATTAtc                                               <  2:1040348/93‑1 (MQ=255)
                                           cgccgcTGAAGTAGTGAGCCGTAATCAGCAAGAGTTACTGCGCCAGGCGCAATCGACCCGTCAACGGCTGGAGATGGCGATGGATTATTAtct                                              <  1:1143416/93‑1 (MQ=255)
                                           cgccgcTGAAGTAGTGAGCCGTAATCAGCAAGAGTTACTGCGCCAGGCGCAATCGACCCGTCAACGGCTGGAGATGGCGATGGATTATTAtct                                              >  2:691827/1‑93 (MQ=255)
                                            gccgcTGAAGTAGTGAGCCGTAATCAGCAAGAGTTACTGCGCCAGGCGCAATCGACCCGTCAACGGCTGGAGATGGCGATGGATTATTAtctc                                             >  1:5257618/1‑93 (MQ=255)
                                            gccgcTGAAGTAGTGAGCCGTAATCAGCAAGAGTTACTGCGCCAGGCGCAATCGACCCGTCAACGGCTGGAGATGGCGATGGATTATTAtctc                                             >  1:5798248/1‑93 (MQ=255)
                                             ccgcTGAAGTAGTGAGCCGTAATCAGCAAGAGTTACTGCGCCAGGCGCAATCGACCCGTCAACGGCTGGAGATGGCGATGGATTATTATCTCg                                            >  2:1960402/1‑93 (MQ=255)
                                             ccgcTGAAGTAGTGAGCCGTAATCAGCAAGAGTTACTGCGCCAGGCGCAATCGACCCGTCAACGGCTGGAGATGGCGATGGATTATTATCTCg                                            <  1:1017444/93‑1 (MQ=255)
                                                 tGAAGTAGTGAGCCGTAATCAGCAAGAGTTACTGCGCCAGGCGCAATCGACCCGTCAACGGCTGGAGATGGCGATGGATTATTATCTCGCCaa                                        >  1:6081179/1‑93 (MQ=255)
                                                 tGAAGTAGTGAGCCGTAATCAGCAAGAGTTACTGCGCCAGCCGCAATCGACCCGTCAACGGCTGGAGATGGCGATGGATTATTATCTCGCCaa                                        <  2:4771271/93‑1 (MQ=255)
                                                     gtagtGAGCCGTAATCAGCAAGAGTTACTGCGCCAGGCGCAATCGACCCGTCAACGGCTGGAGATGGCGATGGATTATTATCTCGCCAACCGc                                    <  2:3541028/93‑1 (MQ=255)
                                                      tagtGAGCCGTAATCAGCAAGAGTTACTGCGCCAGGCGCAATCGACCCGTCAACGGCTGGAGATGGCGATGGATTATTATCTCGCCAACCGca                                   <  1:1419459/93‑1 (MQ=255)
                                                        gtGAGCCGGAATCAGCAAGAGTTACTGCGCCAGGCGCAATCGACCCGTCAACGGCTGGAGATGGCGATGGATTATTATCTCGCCAACCGcaca                                 >  2:6322952/1‑93 (MQ=255)
                                                          gAGCCGTAATCAGCAAGAGTTACTGCGCCAGGCGCAATCGACCCGTCAACGGCTGGAGATGGCGATGGATTATTATCTCGCCAACCGCACACg                               >  2:55084/1‑93 (MQ=255)
                                                           aGCCGTAATCAGCAAGAGTTACTGCGCCAGGCGCAATCGACCCGTCAACGGCTGGAGATGGCGATGGATTATTATCTCGCCAACCGCACACGt                              <  2:5228203/93‑1 (MQ=255)
                                                           aGCCGTAATCAGCAAGAGTTACTGCGCCAGGCGCAATCGACCCGTCAACGGCTGGAGATGGCGATGGATTATTATCTCGCCAACCGCACACGt                              <  1:1976644/93‑1 (MQ=255)
                                                            gCCGTAATCAGCAAGAGTTACTGCGCCAGGCGCAATCGACCCGTCAACGGCTGGAGATGGCGATGGATTATTATCTCGCCAACCGCACACGTc                             >  1:301331/1‑93 (MQ=255)
                                                               gTAATCAGCAAGAGTTACTGCGCCAGGCGCAATCGACCCGTCAACGGCTGGAGATGGCGATGGATTATTATCTCGCCAACCGCACACGTCGCt                          >  2:5580162/1‑93 (MQ=255)
                                                                 aaTCAGCAAGAGTTACTGCGCCAGGCGCAATCGACCCGTCAACGGCTGGAGATGGCGATGGATTATTATCTCGCCAACCGCACACGTCGCttt                        >  2:4934342/1‑93 (MQ=255)
                                                                 aaTCAGCAAGAGTTACTGCGCCAGGCGCAATCGACCCGTCAACGGCTGGAGATGGCGATGGATTATTATCTCGCCAACCGCACACGTCGCttt                        <  1:1811420/93‑1 (MQ=255)
                                                                  aTCAGCAAGAGTTACTGCGCCAGGCGCAATCGACCCGTCAACGGCTGGAGATGGCGATGGATTATTATCTCGCCAACCGCACACGTCGCTTTa                       >  2:289852/1‑93 (MQ=255)
                                                                  aTCAGCAAGAGTTACTGCGCCAGGCGCAATCGACCCGTCAACGGCTGGAGATGGCGATGGATTATTATCTCGCCAACCGCACACGTCGCTTTa                       <  2:1672683/93‑1 (MQ=255)
                                                                   tCAGCAAGAGTTACTGCGCCAGGCGCAATCGACCCGTCAACGGCTGGAGATGGCGATGGATTATTATCTCGCCAACCGCACACGTCGCTTTAc                      <  1:3519345/93‑1 (MQ=255)
                                                                   tCAGCAAGAGTTACTGCGCCAGGCGCAATCGACCCGTCAACGGCTGGAGATGGCGATGGATTATTATCTCGCCAACCGCACACGTCGCTTTAc                      <  2:667821/93‑1 (MQ=255)
                                                                     aGCAAGAGTTACTGCGCCAGGCGCAATCGACCCGTCAACGGCTGGAGATGGCGATGGATTATTATCTCGCCAACCGCACACGTCGCTTTACga                    >  1:2217798/1‑92 (MQ=255)
                                                                     aGCAAGAGTTACTGCGCCAGGCGCAATCGACCCGTCAACGGCTGGAGATGGCGATGGATTATTATCTCGCCAACCGCACACGTCGCTTTACGc                    <  2:2106713/93‑1 (MQ=255)
                                                                      gCAAGAGTTACTGCGCCAGGCGCAATCGACCCGTCAACGGCTGGAGATGGCGATGGATTATTATCTCGCCAACCGCACACGTCGCTTTACGCa                   <  2:1136195/93‑1 (MQ=255)
                                                                      gCAAGAGTTACTGCGCCAGGCGCAATCGACCCGTCAACGGCTGGAGATGGCGATGGATTATTATCTCGCCAACCGCACACGTCGCTTTACGCa                   >  1:3990558/1‑93 (MQ=255)
                                                                         atagTTACTGCGCCAGGCGCAATCGACCCGTCAACGGCTGGAGATGGCGATGGATTATTATCTCGCCAACCGCACACGTCGCTTTACGCAAAt                >  2:4177712/3‑93 (MQ=255)
                                                                          gatttACTGCGCCAGGCGCAATCGACCCGTCAACGGCTGGAGATGGCGATGGATTATTATCTCGCCAACCGCACACGTCGCTTTACGCAAAtt               >  1:2680978/4‑93 (MQ=255)
                                                                          gagTTACTGCGCCAGGCGCAATCGACCCGTCAACGGCTGGAGATGGCGATGGATTATTATCTCGCCAACCGCACACGTCGCTTTACGCAAAtt               >  1:4724820/1‑93 (MQ=255)
                                                                           agTTACTGCGCCAGGCGCAATCGACCCGTCAACGGCTGGAGATGGCGATGGATTATTATCTCGCCAACCGCACACGTCGCTTTACGCAAAtta              >  1:1707818/1‑92 (MQ=255)
                                                                            gTTACTGCGCCAGGCGCAATCGACCCGTCAACGGCTGGAGATGGCGATGGATTATTATCTCGCCAACCGCACACGTCGCTTTACGCAAATtca             >  1:1336932/1‑93 (MQ=255)
                                                                            gTTACTGCGCCAGGCGCAATCGACCCGTCAACGGCTGGAGATGGCGATGGATTATTATCTCGCCAACCGCACACGTCGCTTTACGCAAACtca             >  2:5072282/1‑93 (MQ=255)
                                                                              tACTGCGCCAGGCGCAATCGACCCGTCAACGGCTGGAGATGGCGATGGATTATTATCTCGCCAACCGCACACGTCGCTTTACGCAAATtcatc           >  2:577171/1‑93 (MQ=255)
                                                                               aCTGCGCCAGGCGCAATCGACCCGTCAACGGCTGGAGATGGCGATGGATTATTATCTCGCCAACCGCACACGTCGCTTTACGCAAATtcatca          >  1:892904/1‑93 (MQ=255)
                                                                                 tGCGCCAGGCGCAATCGACCCGTCAACGGCTGGAGATGGCGATGGATTATTATCTCGCCAACCGCACACGTCGCTTTACGCAAATTCATCAcc        <  1:2884897/93‑1 (MQ=255)
                                                                                 tGCGCCAGGCGCAATCGACCCGTCAACGGCTGGAGATGGCGATGGATTATTATCTCGCCAACCGCACACGTCGCTTTACGCAAATTCATCAcc        >  2:2515483/1‑93 (MQ=255)
                                                                                 tGCGCCAGGCGCAATCGACCCGTCAACGGCTGGAGATGGCGATGGATTATTATCTCGCCAACCGCACACGTCGCTTTACGCAAATTCATCAcc        <  2:1530423/93‑1 (MQ=255)
                                                                                    gcCAGGCGCAATCGACCCGTCAACGGCTGGAGATGGCGATGGATTATTATCTCGCCAACCGCACACGTCGCTTTACGCAAATTCATCACCGAt     <  1:390379/93‑1 (MQ=255)
                                                                                    gcCAGGCGCAATCGACCCGTCAACGGCTGGAGATGGCGATGGATTATTATCTCGCCAACCGCACACGTCGCTTTACGCAAATTCATCACCGAt     <  2:3278675/93‑1 (MQ=255)
                                                                                    gcCAGGCGCAATCGACCCGTCAACGGCTGGAGATGGCGATGGATTATTATCTCGCCAACCGCACACGTCGCTTTACGCAAATTCATCACCGAt     <  1:593942/93‑1 (MQ=255)
                                                                                     ccAGGCGCAATCGACCCGTCAACGGCTGGAGATGGCGATGGATTATTATCTCGCCAACCGCACACGTCGCTTTACGCAAATTCATCACCGAtt    <  2:857435/93‑1 (MQ=255)
                                                                                     ccAGGCGCAATCGACCCGTCAACGGCTGGAGATGGCGATGGATTATTATCTCGCCAACCGCACACGTCGCTTTACGCAAATTCATCACCGAtt    >  2:5810047/1‑93 (MQ=255)
                                                                                      cAGGCGCAATCGACCCGTCAACGGCTGGAGATGGCGATGGATTATTATCTCGCCAACCGCACACGTCGCTTTACGCAAATTCATCACCGATTa   <  1:1188593/93‑1 (MQ=255)
                                                                                      cAGGCGCAATCGACCCGTCAACGGCTGGAGATGGCGATGGATTATTATCTCGCCAACCGCACACGTCGCTTTACGCAAATTCATCACCGATTa   <  1:2829780/93‑1 (MQ=255)
                                                                                      cAGGCGCAATCGACCCGTCAACGGCTGGAGATGGCGATGGATTATTATCTCGCCAACCGCACACGTCGCTTTACGCAAATTCATCACCGATTa   <  2:2972552/93‑1 (MQ=255)
                                                                                       aGGCGCAATCGACCCGTCAACGGCTGGAGATGGCGATGGATTATTATCTCGCCAACCGCACACGTCGCTTTACGCAAATTCATCACCGATTac  <  1:774777/93‑1 (MQ=255)
                                                                                          |                                                                                         
TTGCCGATTTTGTTGCCGATCTGCGTGCGCCAACGCCGTCTGCCGCCGCTGAAGTAGTGAGCCGTAATCAGCAAGAGTTACTGCGCCAGGTGCAATCGACCCGTCAACGGCTGGAGATGGCGATGGATTATTATCTCGCCAACCGCACACGTCGCTTTACGCAAATTCATCACCGATTAC  >  NC_000913/2632982‑2633161

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
CCATTGCCGATTTTGTTGCCGATCTGCGTGCGCCAACGCCGTCTGCCGCCGCTGAAGTAGTGAGCCGTAATCAGCAAGAGTTACTGCGCCAGGTGCAATCGACCCGTCAACGGCTGGAGATGGCGATGGATTATTATCTCGCCAACCGCACACGTCGCTTTACGCAAATTCATCACCGATTACAG  >  NC_000913/2632979‑2633163
                                                                                             |                                                                                           
CCATTGCCGATTTTGTTGCCGATCTGCGTGCGCCAACGCCGTCTGCCGCCGCTGAAGTAGTGAGCCGTAATCAGCAAGAGTTACTGCGCCAGGC                                                                                             >  SRR4302537.4649005/1‑94 (MQ=60)
CCATTGCCGATTTTGTTGCCGATCTGCGTGCGCCAACGCCGTCTGCCGCCGCTGAAGTAGTGAGCCGTAATCAGCAAGAGTTACTGCGCCAGGC                                                                                             <  SRR4302537.1771783/94‑1 (MQ=60)
 CATTGCCGATTTTGTTGCCGATCTGCGTGCGCCAACGCCGTCTGCCGCCGCTGAAGTAGTGAGCCGTAATCAGCAAGAGTTACTGCGCCAGGCG                                                                                            >  SRR4302537.3544484/1‑94 (MQ=60)
 CATTGCCGATTTTGTTGCCGATCTGCGTGCGCCAACGCCGTCTGCCGCCGCTGAAGTAGTGAGCCGTAATCAGCAAGAGTTACTGCGCCAGGCG                                                                                            <  SRR4302537.2628052/94‑1 (MQ=60)
    TGCCGATTTTGTTGCCGATCTGCGTGCGCCAACGCCGTCTGCCGCCGCTGAAGTAGTGAGCCGTAATCAGCAAGAGTTACTGCGCCAGGCGCAA                                                                                         <  SRR4302537.231258/94‑1 (MQ=60)
      CCGATTTTGTTGCCGATCTGCGTGCGCCAACGCCGTCTGCCGCCGCTGAAGTAGTGAGCCGTAATCAGCAAGAGTTACTGCGCCAGGCGCAATC                                                                                       <  SRR4302537.1486992/94‑1 (MQ=60)
       CGATTTTGTTGCCGATCTGCGTGCGCCAACGCCGTCTGCCGCCGCTGAAGTAGTGAGCCGTAATCAGCAAGAGTTACTGCGCCAGGCGCAATCG                                                                                      <  SRR4302537.515694/94‑1 (MQ=60)
       CGATTTTGTTGCCGATCTGCGTGCGCCAACGCCGTCTGCCGCCGCTGAAGTAGTGAGCCGTAATCAGCAAGAGTTACTGCGCCAGGCGCAATCG                                                                                      >  SRR4302537.657643/1‑94 (MQ=60)
         ATTTTGTTGCCGATCTGCGTGCGCCAACGCCGTCTGCCGCCGCTGAAGTAGTGAGCCGTAATCAGCAAGAGTTACTGCGCCAGGCGCAATCGAC                                                                                    >  SRR4302537.4338984/1‑94 (MQ=60)
         ATTTTGTTGCCGATCTGCGTGCGCCAACGCCGTCTGCCGCCGCTGAAGTAGTGAGCCGTAATCAGCAAGAGTTACTGCGCCAGGCGCAATCGAC                                                                                    <  SRR4302537.398150/94‑1 (MQ=60)
         ATTTTGTTGCCGATCTGCGTGCGCCAACGCCGTCTGCCGCCGCTGAAGTAGTGAGCCGTAATCAGCAAGAGTTACTGCGCCAGGCGCAATCGAC                                                                                    >  SRR4302537.3437983/1‑94 (MQ=60)
          TTTTGTTGCCGATCTGCGTGCGCCAACGCCGTCTGCCGCCGCTGAAGTAGTGAGCCGTAATCAGCAAGAGTTACTGCGCCAGGCGCAATCGACC                                                                                   >  SRR4302537.3133499/1‑94 (MQ=60)
           TTTGTTGCCGATCTGCGTGCGCCAACGCCGTCTGCCGCCGCTGAAGTAGTGCGCCGTAATCAGCAAGAGTTACTGCGCCAGGCGCAATCGACCC                                                                                  >  SRR4302537.4302152/1‑94 (MQ=60)
           TTTGTTGCCGATCTGCGTGCGCCAACGCCGTCTGCCGCCGCTGAAGTAGTGAGCCGTAATCAGCAAGAGTTACTGCGCCAGGCGCAATCGACCC                                                                                  >  SRR4302537.3816743/1‑94 (MQ=60)
           TTGGTGGCCGATCTGCGTGCGCCAACGCCGTCTGCCGCCGCTGAAGTAGTGAGCCGTAATCAGCAAGAGTTACTGCGCCAGGCGCAATCGACCC                                                                                  <  SRR4302537.2001162/94‑1 (MQ=60)
            TTGTTGCCGATCTGCGTGCGCCAACGCCGTCTGCCGCCGCTGAAGTAGTGAGCCGTAATCAGCAAGAGTTACTGCGCCAGGCGCAATCGACCCG                                                                                 <  SRR4302537.4357698/94‑1 (MQ=60)
               TTGCCGATCTGCGTGCGCCAACGCCGTCTGCCGCCGCTGAAGTAGTGAGCCGTAATCAGCAAGAGTTACTGCGCCAGGCGCAATCGACCCGTCA                                                                              <  SRR4302537.1195685/94‑1 (MQ=60)
                TGCCGATCTGCGTGCGCCAACGCCGTCTGCCGCCGCTGAAGTAGTGAGCCGTAATCAGCAAGAGTTACTGCGCCAGGCGCAATCGACCCGTCAA                                                                             >  SRR4302537.5616361/1‑94 (MQ=60)
                 GCCGATCTGCGTGCGCCAACGCCGTCTGCCGCCGCTGAAGTAGTGAGCCGTAATCAGCAAGAGTTACTGCGCCAGGCGCAATCGACCCGTCAAC                                                                            <  SRR4302537.1516596/94‑1 (MQ=60)
                 GCCGATCTGCGTGCGCCAACGCCGTCTGCCGCCGCTGAAGTAGTGAGCCGTAATCAGCAAGAGTTACTGCGCCAGGCGCAATCGACCCGTCAAC                                                                            <  SRR4302537.1602939/94‑1 (MQ=60)
                 GCCGATCTGCGGGCCCCAACGCCGTCTGCCGCCGCTGAAGTAGTGAGCCGTAATCAGCAAGAGTTACTGCGCCAGGCGCAATCGACCCGTCAAC                                                                            <  SRR4302537.4282209/94‑1 (MQ=60)
                  CCGATCTGCGTGCGCCAACGCCGTCTGCCGCCGCTGAAGTAGTGAGCCGTAATCAGCAAGAGTTACTGCGCCAGGCGCAATCGACCCGTCAACG                                                                           <  SRR4302537.4539026/94‑1 (MQ=60)
                  CCGATCTGCGTGCGCCAACGCCGTCTGCCGCCGCTGAAGTAGTGAGCCGTAATCAGCAAGAGTTACTGCGCCAGGCGCAATCGACCCGTCAACG                                                                           <  SRR4302537.6302390/94‑1 (MQ=60)
                  CCGATCTGCGTGCGCCAACGCCGTCTGCCGCCGCTGAAGTAGTGAGCCGTAATCAGCAAGAGTTACTGCGCCAGGCGCAATCGACCCGTCAACG                                                                           >  SRR4302537.6014491/1‑94 (MQ=60)
                  CCGATCTGCGTGCGCCAACGCCGTCTGCCGCCGCTGAAGTAGTGAGCCGTAATCAGCAAGAGTTACTGCGCCAGGCGCAATCGACCCGTCAACG                                                                           <  SRR4302537.2852650/94‑1 (MQ=60)
                   CGATCTGCGTGCGCCACCGCCGTCTGCCGCCGCTGAAGTAGTGAGCCGTAATCAGCAAGAGTTACTGCGCCAGGCGCAATCGACCCGTCAACGG                                                                          <  SRR4302537.3644486/94‑1 (MQ=60)
                   CGATCTGCGTGCGCCAACGCCGTCTGCCGCCGCTGAAGTAGTGAGCCGTAATCAGCAAGAGTTACTGCGCCAGGCGCAATCGACCCGTCAACGG                                                                          >  SRR4302537.762294/1‑94 (MQ=60)
                    GATCTGCGTGCGCCAACGCCGTCTGCCGCCGCTGAAGTAGTGAGCCGTAATCAGCAAGAGTTACTGCGCCAGGCGCAATCGACCCGTCAACGGC                                                                         >  SRR4302537.2552033/1‑94 (MQ=60)
                    GATCTGCGTGCGCCAACGCCGTCTGCCGCCGCTGAAGTAGTGAGCCGTAATCAGCAAGAGTTACTGCGCCAGGCGCAATCGACCCGTCAACGGC                                                                         <  SRR4302537.3198160/94‑1 (MQ=60)
                    GATCTGCGTGCGCCAACGCCGTCTGCCGCCGCTGAAGTAGTGAGCCGTAATCAGCAAGAGTTACTGCGCCAGGCGCAATCGACCCGTCAACGGC                                                                         >  SRR4302537.3662476/1‑94 (MQ=60)
                     ATCTGCGTGCGCCACCGCCGTCTGCCGCCGCTGAAGTAGTGAGCCGTAATCAGCAAGAGTTACTGCGCCAGGCGCAATCGACCCGTCAACGGCT                                                                        <  SRR4302537.2134643/94‑1 (MQ=60)
                     ATCTGCGTGCGCCAACGCCGTCTGCCGCCGCTGAAGTAGTGAGCCGTAATCAGCAAGAGTTACTGCGCCAGGCGCAATCGACCCGTCAACGGCT                                                                        >  SRR4302537.180368/1‑94 (MQ=60)
                     ATCTGCGTGCGCCAACGCCGTCTGCCGCCGCTGAAGTAGTGAGCCGTAATCAGCAAGAGTTACTGCGCCAGGCGCAATCGACCCGTCAACGGCT                                                                        >  SRR4302537.3790595/1‑94 (MQ=60)
                     ATCTGCGTGCGCCAACGCCGTCTGCCGCCGCTGAAGTAGTGAGCCGTAATCAGCAAGAGTTACTGCGCCAGGCGCAATCGACCCGTCAACGGCT                                                                        >  SRR4302537.690062/1‑94 (MQ=60)
                       CTGCGTGCGCCAACGCCGTCTGCCGCCGCTGAAGTAGTGAGCCGTAATCAGCAAGAGTTACTGCGCCAGGCGCAATCGACCCGTCAACGGCTGG                                                                      >  SRR4302537.351346/1‑94 (MQ=60)
                       CTGCGTGCGCCAACGCCGTCTGCCGCCGCTGAAGTAGTGAGCCGTAATCAGCAAGAGTTACTGCGCCAGGCGCAATCGACCCGTCAACGGCTGG                                                                      >  SRR4302537.5500642/1‑94 (MQ=60)
                         GCGTGCGCCAACGCCGTCTGCCGCCGCTGAAGTAGTGAGCCGTAATCAGCAAGAGTTACTGCGCCAGGCGCAATCGACCCGTCAACGGCTGGAG                                                                    <  SRR4302537.5494221/94‑1 (MQ=60)
                           GTGCGCCAACGCCGTCTGCCGCCGCTGAAGTAGTGAGCCGTAATCAGCAAGAGTTACTGCGCCAGGCGCAATCGACCCGTCAACGGCTGGAGAT                                                                  <  SRR4302537.1522390/94‑1 (MQ=60)
                           GTGCCCCAACCCCGTCTGCCGCCGCTGAAGTAGTGAGCCGTAATCAGCAAGAGTTACTGCGCCAGGCGCAATCGACCCGTCAACGGCTGGAGAT                                                                  <  SRR4302537.3437246/94‑1 (MQ=60)
                            TGCGCCAACGCCGTCTGCCGCCGCTGAAGTAGTGAGCCGTAATCAGCAAGAGTTACTGCGCCAGGCGCAATCGACCCGTCAACGGCTGGAGATG                                                                 <  SRR4302537.5011266/94‑1 (MQ=60)
                            TGCGCCAACGCCGTCTGCCGCCGCTGAAGTAGTGAGCCGTAATCAGCAAGAGTTACTGCGCCAGGCGCAATCGACCCGTCAACGGCTGGAGATG                                                                 <  SRR4302537.3385917/94‑1 (MQ=60)
                              CGCCAACGCCGTCTGCCGCCGCTGAAGTAGTGAGCCGTAATCAGCAAGAGTTACTGCGCCAGGCGCAATCGACCCGTCAACGGCTGGAGATGGC                                                               <  SRR4302537.5394197/94‑1 (MQ=60)
                               GCCAACGCCGTCTGCCGCCGCTGAAGTAGTGAGCCGTAATCAGCAAGAGTTACTGCGCCAGGCGCAATCGACCCGTCAACGGCTGGAGATGGCG                                                              <  SRR4302537.768588/94‑1 (MQ=60)
                               GCCAACGCCGTCTGCCGCCGCTGAAGTAGTGAGCCGTAATCAGCAAGAGTTACTGCGCCAGGCGCAATCGACCCGTCAACGGCTGGAGATGGCG                                                              >  SRR4302537.3561519/1‑94 (MQ=60)
                                CCAGCGCTGTCTGCCGCCGCTGAAGTAGTGAGCCGTAATCAGCAAGAGTTACTGCGCCAGGCGCAATCGACCCGTCAACGGCTGGAGATGGCGA                                                             <  SRR4302537.2621710/94‑1 (MQ=60)
                                 CACCGCCGTCTGCCGCCGCTGAAGTAGTGAGCCGTAATCTGCAAGAGTTACTGCGCCAGGCGCAATCGACCCGTCAACGGCTGGAGATGGCGAT                                                            <  SRR4302537.220188/94‑1 (MQ=60)
                                 CAACGCCGTCTGCCGCCGCTGAAGTAGTGAGCCGTAATCAGCAAGAGTTACTGCGCCAGGCGCAATCGACCCGTCAACGGCTGGAGATGGCGAT                                                            >  SRR4302537.639548/1‑94 (MQ=60)
                                  AACGCCGTCTGCCGCCGCTGAAGTAGTGAGCCGTAATCAGCAAGAGTTACTGCGCCAGGCGCAATCGACCCGTCAACGGCTGGAGATGGCGATG                                                           >  SRR4302537.3115754/1‑94 (MQ=60)
                                  AACGCCGTCTGCCGCCGCTGAAGTAGTGAGCCGTAATCAGCAAGAGTTACTGCGCCAGGCGCAATCGACCCGTCAACGGCTGGAGATGGCGATG                                                           <  SRR4302537.150944/94‑1 (MQ=60)
                                  AACGCCGTCGGCCGCCGCTGAAGTAGTGAGCCGTAATCAGCAAGAGTTACTGCGCCAGGCGCAATCGACCCGTCAACGGCTGGAGATGGCGATG                                                           <  SRR4302537.641474/94‑1 (MQ=60)
                                   ACGCCGTCTGCCGCCGCTGAAGTAGTGAGCCGTAATCAGCAAGAGCTACTGCGCCAGGCGCAATCGACCCGTCAACGGCTGGAGATGGCGATGG                                                          >  SRR4302537.5473041/1‑94 (MQ=60)
                                   AAGCCGTCTGCCGCCGCTGAAGTAGTGAGCCGTAATCAGCAAGAGTTACTGCGCCAGGCGCAATCGACCCGTCAACGGCTGGAGATGGCGATGG                                                          <  SRR4302537.1210097/94‑1 (MQ=60)
                                    CGCCTTCTGCCGCCGCTGAAGTAGTGAGCCGTAATCAGCAAGAGTTACTGCGCCAGGCGCAATCGACCCGTCAACGGCTGGAGATGGCGATGGA                                                         <  SRR4302537.1789502/94‑1 (MQ=60)
                                    CGCCGTCTGCCGCCGCTGAAGTAGTGAGCCGTAATCAGCAAGAGTTACTGCGCCAGGCGCAATCGACCCGTCAACGGCTGGAGATGGCGATGGA                                                         >  SRR4302537.3013725/1‑94 (MQ=60)
                                    CGCCGTCTGCCGCCGCTGAAGTAGTGAGCCGTAATCAGCAAGAGTTACTGCGCCAGGCGCAATCGACCCGTCAACGGCTGGAGATGGCGATGGA                                                         <  SRR4302537.5028208/94‑1 (MQ=60)
                                      CCGTCTGCCGCCGCTGAAGTAGTGAGCCGTAATCAGCAAGAGTTACTGCGCCAGGCGCAATCGACCCGTCAACGGCTGGAGATGGCGATGGATT                                                       >  SRR4302537.353271/1‑94 (MQ=60)
                                       CGTCTGCCGCCGCTGAAGTAGTGAGCCGTAATCAGCAAGAGTTACTGCGCCAGGCGCAATCGACCCGTCAACGGCTGGAGATGGCGATGGATTA                                                      <  SRR4302537.1649661/94‑1 (MQ=60)
                                       CGTCTGCCGCCGCTGAAGTAGTGAGCCGTAATCAGCAAGAGTTACTGCGCCAGGCGCAATCGACCCGTCAACGGCTGGAGATGGCGATGGATTA                                                      <  SRR4302537.874012/94‑1 (MQ=60)
                                        GTCTGCCGCCGCTGAAGTAGTGAGCCGTAATCAGCAAGAGTTACTGCGCCAGGCGCAATCGACCCGTCAACGGCTGGAGATGGCGATGGATTAT                                                     <  SRR4302537.4876914/94‑1 (MQ=60)
                                        GTCTGCCGCCGCTGAAGTAGTGAGCCGTAATCAGCAAGAGTTACTGCGCCAGGCGCAATCGACCCGTCAACGGCTGGAGATGGCGATGGATTAT                                                     >  SRR4302537.3703495/1‑94 (MQ=60)
                                        GTCTGCCGCCGCTGAAGTAGTGAGCCGTAAGCAGCAAGAGTTACTGCGCCAGGCGCAATCGACCCGTCAACGGCTGGAGATGGCGATGGATTAT                                                     <  SRR4302537.3131791/94‑1 (MQ=60)
                                         TCTGCCGCCGCTGAAGTAGTGAGCCGTAATCAGCAGGAGTTACTGCGCCAGGCGCAATCGACCCGTCAACGGCTGGAGATGGCGATGGATTATT                                                    <  SRR4302537.1695693/94‑1 (MQ=60)
                                         TCTGCCGCCGCTGAAGTAGTGAGCCGTAATCAGCAAGAGTTACTGCGCCAGGCGCAATCGACCCGTCAACGGCTGGAGATGGCGATGGATTATT                                                    <  SRR4302537.2001844/94‑1 (MQ=60)
                                          CTGCCGCCGCTGAAGTAGTGAGCCGTAATCAGCAAGAGTTACTGCGCCAGGCGCAATCGACCCGTCAACGGCTGGAGATGGCGATGGGTTATTA                                                   >  SRR4302537.1598418/1‑94 (MQ=60)
                                          CTGCCGCCGCTGAAGTAGTGAGCCGTAATCAGCAAGAGTTACTGCGCCAGGCGCAATCGACCCGTCAACGGCTGGAGATGGCGATGGATTATTA                                                   >  SRR4302537.1490124/1‑94 (MQ=60)
                                           TGCCGCCGCTGAAGTAGTGAGCCGTAATCAGCAAGAGTTACTGCGCCAGGCGCAATCGACCCGTCAACGGCTGGAGATGGCGATGGATTATTAT                                                  <  SRR4302537.296769/94‑1 (MQ=60)
                                           TGCCGCCGCTGAAGTAGTGAGCCGTAATCAGCAAGAGTTACTGCGCCAGGCGCAATCGACCCGTCAACGGCTGGAGATGGCGATGGATTATTAT                                                  <  SRR4302537.1813671/94‑1 (MQ=60)
                                            GCCGCCGCTGAAGTAGTGAGCCGTAATCAGCAAGAGTTACTGCGCCAGGCGCAATCGACCCGTCAACGGCTGGAGATGGCGATGGATTATTATC                                                 >  SRR4302537.2021275/1‑94 (MQ=60)
                                             CCGCCGCTGAAGTAGTGAGCCGTAATCAGCAAGAGTTACTGCGCCAGGCGCAATCGACCCGTCAACGGCTGGAGATGGCGATGGATTATTATCT                                                <  SRR4302537.1786569/94‑1 (MQ=60)
                                              CGCCGCTGAAGTAGTGAGCCGTAATCAGCAAGAGTTACTGCGCCAGGCGCAATCGACCCGTCAACGGCTGGAGATGGCGATGGATTATTATCTC                                               <  SRR4302537.1151367/94‑1 (MQ=60)
                                              CGCCGCTGAAGTAGTGAGCCGTAATCAGCAAGAGTTACTGCGCCAGGCGCAATCGACCCGTCAACGGCTGGAGATGGCGATGGATTATTATCTC                                               >  SRR4302537.5838000/1‑94 (MQ=60)
                                              CGCCGCTGAAGTAGTGAGCCGTAATCAGCAAGAGTTACTGCGCCAGGCGCAATCGACCCGTCAACGGCTGGAGATGGCGATGGATTATTATCTC                                               >  SRR4302537.5293800/1‑94 (MQ=60)
                                               GCCGCTGAAGTAGTGAGCCGTAATCAGCAAGAGTTACTGCGCCAGGCGCAATCGACCCGTCAACGGCTGGAGATGGCGATGGATTATTATCTCG                                              <  SRR4302537.3913405/94‑1 (MQ=60)
                                                CCGCTGAAGTAGTGAGCCGTAATCAGCAAGAGTTACTGCGCCAGGCGCAATCGACCCGTCAACGGCTGGAGATGGCGATGGATTATTATCTCGC                                             <  SRR4302537.1024447/94‑1 (MQ=60)
                                                 CGCTGAAGTAGTGAGCCGTAATCAGCAAGAGTTACTGCGCCAGGCGCAATCTACCCGTCAACGGCTGGAGATGGCGATGGATTATTATCTCGCC                                            >  SRR4302537.5428899/1‑94 (MQ=60)
                                                 CGCTGAAGTAGTGAGCCGTAATCAGCAAGAGTTACTGCGCCAGGCGCAATCGACCCGTCAACGGCTGGAGATGGCGATGGATTATTATCTCGCC                                            >  SRR4302537.4569515/1‑94 (MQ=60)
                                                   CTGAAGTAGTGAGCCGTAATCAGCAAGAGTTACTGCGCCAGGCGCAATCGACCCGTCAACGGCTGGAGATGGCGATGGATTATTATCTCGCCAA                                          >  SRR4302537.6122964/1‑94 (MQ=60)
                                                   CTGAAGTAGTGAGCCGTAATCAGCAAGAGTTACTGCGCCAGGCGCAATCGACCCGTCAACGGCTGGAGATGGCGATGGATTATTATCTCGCCAA                                          >  SRR4302537.2600050/1‑94 (MQ=60)
                                                    TGAAGTAGTGAGCCGTAATCAGCAAGAGTTACTGCGCCAGGCGCAATCGACCCGTCAACGGCTGGAGATGGCGATGGATTATTATCTCGCCAAC                                         >  SRR4302537.1402072/1‑94 (MQ=60)
                                                       AGTAGTGAGCCGTAATCAGCAAGAGTTACTGCGCCAGGCGCAATCGACCCGTCAACGGCTGGAGATGGCGATGGATTATTATCTCGCCAACCGC                                      >  SRR4302537.1197586/1‑94 (MQ=60)
                                                       AGTAGTGAGCCGTAATCAGCAAGAGTTACTGCGCCAGGCGCAATCGACCCGTCAACGGCTGGAGATGGCGATGGATTATTATCTCGCCAACCGC                                      >  SRR4302537.3021784/1‑94 (MQ=60)
                                                        GTAGTGAGCCGTAATCAGCAAGAGTTACTGCGCCAGGCGCAATCGACCCGTCAACGGCTGGAGATGGCGATGGATTATTATCTCGCCAACCGCA                                     <  SRR4302537.595323/94‑1 (MQ=60)
                                                         TAGTGAGCCGTAATCAGCAAGAGTTACTGCGCCAGGCGCAATCGACCCGTCAACGGCTGGAGATGGCGATGGATTATTATCTCGCCAACCGCAC                                    <  SRR4302537.1429336/94‑1 (MQ=60)
                                                          AGTGAGCCGTAATCAGCAAGAGTTACTGCGCCAGGCGCAATCGACCCGTCAACGGCTGGAGATGGCGATGGATTATTATCTCGCCAACCGCACA                                   >  SRR4302537.2586290/1‑94 (MQ=60)
                                                            TGAGCTGTAATCAGCAAGAGTTACTGCGCCAGGTGCAATCGACCCGTCAACGGCTGAAGATGGCGATGGATTATTATCTCGCCAACCGCACACG                                 <  SRR4302537.2710687/94‑1 (MQ=60)
                                                              AGCCGTAATCAGCAAGAGTTACTGCGCCAGGCGCAATCGACCCGTCAACGGCTGGAGATGGCGATGGATTATTATCTCGCCAACCGCACACGTC                               >  SRR4302537.303430/1‑94 (MQ=60)
                                                              AGCCGTAATCAGCAAGAGTTACTGCGCCAGGCGCAATCGACCCGTCAACGGCTGGAGATGGCGATGGATTATTATCTCGCCAACCGCACACGTC                               <  SRR4302537.6389443/94‑1 (MQ=60)
                                                              AGCCGTAATCAGCAAGAGTTACTGCGCCAGGCGCAATCGACCCGTCAACGGCTGGAGATGGCGATGGATTATTATCTCGCCAACCGCACACGTC                               <  SRR4302537.1990380/94‑1 (MQ=60)
                                                              AGCCGTAATCAGCAAGAGTTACTGCGCCAGGCGCAATCGACCCGTCAACGGCTGGAGATGGCGATGGATTATTATCTCGCCAACCGCACACGTC                               <  SRR4302537.5225791/94‑1 (MQ=60)
                                                               GCCGTAATCAGCAAGAGTTACTGCGCCAGGCGCAATCGACCCGTCAACGGCTGGAGATGGCGATGGATTATTATCTCGCCAACCGCACACGTCG                              <  SRR4302537.5464813/94‑1 (MQ=60)
                                                               GCCGTAATCAGCAAGAGTTACTGCGCCAGGCGCAATCGACCCGTCAACGGCTGGAGATGGCGATGGATTATTATCTCGCCAACCGCACACGTCG                              <  SRR4302537.5634649/94‑1 (MQ=60)
                                                                 CGTAATCAGCAAGAGTTACTGCGCCAGGCGCAATCGACCCGTCAACGGCTGGAGATGGCGATGGATTATTATCTCGCCAACCGCACACGTCGCT                            >  SRR4302537.1213687/1‑94 (MQ=60)
                                                                  GTAATCAGCAAGAGTTACTGCGCCAGGCGCAATCGACCCGTCAACGGCTGGAGATGGCGATGGATTATTATCTCGCCAACCGCACACGTCGCTT                           >  SRR4302537.840686/1‑94 (MQ=60)
                                                                   TAATCAGCAAGAGTTACTGCGCCAGGCGCAATCGACCCGTCAACGGCTGGAGATGGCGATGGATTATTATCTCGCCAACCGCACACGTCGCTTT                          <  SRR4302537.916893/94‑1 (MQ=60)
                                                                   GAATCAGCAAGAGTTACTCGGCCAGGCGCAATCGACCCGTCAACGGCTGGAGATGGCGATGGATTATTATCTCGCCAACCGCACACGTCGCTTT                          <  SRR4302537.2121695/94‑1 (MQ=60)
                                                                    AATCAGCAAGAGTTACTGCGCCAGGCGCAATCGACCCGTCAACGGCTGGAGATGGCGATGGATTATTATCTCGCCAACCGCACACGTCGCTTTA                         <  SRR4302537.1824048/94‑1 (MQ=60)
                                                                      TCAGCAAGAGTTACTGCGCCAGGCGCAATCGACCCGTCAACGGCTGGAGATGGCGATGGATTATTATCTCGCCAACCGCACACGTCGCTTTACG                       <  SRR4302537.3544247/94‑1 (MQ=60)
                                                                       CAGCAAGAGTTACTGCGCCAGGCGCAATCGACCCGTCAACGGCTGGAGATGGCGATGGATTATTATCTCGCCAACCGCACACGTCGCTTTACGA                      >  SRR4302537.2233434/1‑94 (MQ=60)
                                                                        AGCAAGAGTTACTGCGCCAGGCGCAATCGACCCGTCAACGGCTGGAGATGGCGATGGATTATTATCTCGCCAACCGCACACGTCGCTTTACGCA                     >  SRR4302537.4018501/1‑94 (MQ=60)
                                                                        AGCAAGAGTTACTGCGCCAGGCGCAATCGACCCGTCAACGGCTGGAGATGGCGATGGATTATTATCTCGCCAACCGCACACGTCGCTTTACGCA                     <  SRR4302537.6189618/94‑1 (MQ=60)
                                                                           AAGAGTTACTGCGCCAGGCGCAATCGACCCGTCAACGGCTGGAGATGGCGATGGATTATTATCTCGCCAACCGCACACGTCGCTTTACGCAAAT                  >  SRR4302537.3197121/1‑94 (MQ=60)
                                                                            AGATTTACTGCGCCAGGCGCAATCGACCCGTCAACGGCTGGAGATGGCGATGGATTATTATCTCGCCAACCGCACACGTCGCTTTACGCAAATT                 >  SRR4302537.2699697/1‑94 (MQ=60)
                                                                            AGAGTTACTGCGCCAGGCGCAATCGACCCGTCAACGGCTGGAGATGGCGATGGATTATTATCTCGCCAACCGCACACGTCGCTTTACGCAAATT                 >  SRR4302537.4757479/1‑94 (MQ=60)
                                                                            AGAGTTACTGCGCCAGGCGCAATCGACCCGTCAACGGCTGGAGATGGCGATGGATTATTATCTCGCCAACCGCACACGTCGCTTTACGCAAATT                 <  SRR4302537.2685173/94‑1 (MQ=60)
                                                                             GAGTTACTGCGCCAGGCGCAATCGACCCGTCAACGGCTGGAGATGGCGATGGATTATTATCTCGCCAACCGCACACGTCGCTTTACGCAAATTA                >  SRR4302537.1719755/1‑94 (MQ=60)
                                                                              AGTTACTGCGCCAGGCGCAATCGACCCGTCAACGGCTGGAGATGGCGATGGATTATTATCTCGCCAACCGCACACGTCGCTTTACGCAAATTCA               >  SRR4302537.3795634/1‑94 (MQ=60)
                                                                              AGTTACTGCGCCAGGCGCAATCGACCCGTCAACGGCTGGAGATGGCGATGGATTATTATCTCGCCAACCGCACACGTCGCTTTACGCAAATTCA               >  SRR4302537.1346245/1‑94 (MQ=60)
                                                                               GTTACTGCGCCAGGCGCAATCGACCCGTCAACGGCTGGAGATGGCGATGGATTATTATCTCGCCAACCGCACACGTCGCTTTACGCAAATTCAT              >  SRR4302537.1945865/1‑94 (MQ=60)
                                                                                 TACTGCGCCAGGCGCAATCGACCCGTCAACGGCTGGAGATGGCGATGGATTATTATCTCGCCAACCGCACACGTCGCTTTACGCAAATTCATCA            >  SRR4302537.899066/1‑94 (MQ=60)
                                                                                 TACTGCGCCAGGCGCAATCGACCCGTCAACGGCTGGAGATGGCGATGGATTATTATCTCGCCAACCGCACACGTCGCTTTACGCAAATTCATCA            >  SRR4302537.144693/1‑94 (MQ=60)
                                                                                   CTGCGCCAGGCGCAATCGACCCGTCAACGGCTGGAGATGGCGATGGATTATTATCTCGCCAACCGCACACGTCGCTTTACGCAAATTCATCACC          >  SRR4302537.5594945/1‑94 (MQ=60)
                                                                                   CTGCGCCAGGCGCAATCGACCCGTCAACGGCTGGAGATGGCGATGGATTATTATCTCGCCAACCGCACACGTCGCTTTACGCAAATTCATCACC          >  SRR4302537.6216365/1‑94 (MQ=60)
                                                                                    TGCGCCAGGCGCAATCGACCCGTCAACGGCTGGAGATGGCGATGGATTATTATCTCGCCAACCGCACACGTCGCTTTACGCAAATTCATCACCG         <  SRR4302537.2905143/94‑1 (MQ=60)
                                                                                     GCGCCAGGCGCAATCGACCCGTCAACGGCTGGAGATGGCGATGGATTATTATCTCGCCAACCGCACACGTCGCTTTACGCAAATTCATCACCGA        >  SRR4302537.424254/1‑94 (MQ=60)
                                                                                       GCCAGGCGCAATCGACCCGTCAACGGCTGGAGATGGCGATGGATTATTATCTCGCCAACCGCACACGTCGCTTTACGCAAATTCATCACCGATT      <  SRR4302537.598003/94‑1 (MQ=60)
                                                                                       GCCAGGCGCAATCGACCCGTCAACGGCTGGAGATGGCGATGGATTATTATCTCGCCAACCGCACACGTCGCTTTACGCAAATTCATCACCGATT      <  SRR4302537.393077/94‑1 (MQ=60)
                                                                                        CCAGGCGCAATCGACCCGTCAACGGCTGGAGATGGCGATGGATTATTATCTCGCCAACCGCACACGTCGCTTTACGCAAATTCATCACCGATTA     <  SRR4302537.1094808/94‑1 (MQ=60)
                                                                                        CCAGGCGCAATCGACCCGTCAACGGCTGGAGATGGCGATGGATTATTATCTCGCCAACCGCACACGTCGCTTTACGCAAATTCATCACCGATTA     >  SRR4302537.5372766/1‑94 (MQ=60)
                                                                                         CAGGCGCAATCGACCCGTCAACGGCTGGAGATGGCGATGGATTATTATCTCGCCAACCGCACACGTCGCTTTACGCAAATTCATCACCGATTAC    <  SRR4302537.1196849/94‑1 (MQ=60)
                                                                                         CAGGCGCAATCGACCCGTCAACGGCTGGAGATGGCGATGGATTATTATCTCGCCAACCGCACACGTCGCTTTACGCAAATTCATCACCGATTAC    <  SRR4302537.2849550/94‑1 (MQ=60)
                                                                                         CAGGCGCAATCGACCCGTCAACGGCTGGAGATGGCGATGGATTATTATCTCGCCAACCGCACACGTCGCTTTACGCAAATTCATCACCGATTAC    >  SRR4302537.4506924/1‑94 (MQ=60)
                                                                                          AGGCGCAATCGACCCGTCAACGGCTGGAGATGGCGATGGATTATTATCTCGCCAACCGCACACGTCGCTTTACGCAAATTCATCACCGATTACA   <  SRR4302537.780085/94‑1 (MQ=60)
                                                                                           GGCGCAATCGACCCGTCAACGGCTGGAGATGGCGATGGATTATTATCTCGCCAACCGCACACGTCGCTTTACGCAAATTCATCACCGATTACAG  <  SRR4302537.1908765/94‑1 (MQ=60)
                                                                                           GGCGCAATCGACCCGTCAACGGCTGGAGATGGCGATGGATTATTATCTCGCCAACCGCACACGTCGCTTTACGCAAATTCATCACCGATTACAG  >  SRR4302537.1357094/1‑94 (MQ=60)
                                                                                           GGCGCAATCGACCCGTCAACGGCTGGAGATGGCGATGGATTATTATCTCGCCAACCGCACACGTCGCTTTACGCAAATTCATCACCGATTACAG  <  SRR4302537.2657327/94‑1 (MQ=60)
                                                                                             |                                                                                           
CCATTGCCGATTTTGTTGCCGATCTGCGTGCGCCAACGCCGTCTGCCGCCGCTGAAGTAGTGAGCCGTAATCAGCAAGAGTTACTGCGCCAGGTGCAATCGACCCGTCAACGGCTGGAGATGGCGATGGATTATTATCTCGCCAACCGCACACGTCGCTTTACGCAAATTCATCACCGATTACAG  >  NC_000913/2632979‑2633163

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 2 ≤ ATCG/ATCG < 23 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑