Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I2 R1 882 254.4 12957588 98.0% 12698436 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,689,613 A→G intergenic (‑432/+63) yfhK ← / ← purL putative 2‑component sensor protein/phosphoribosylformyl‑glycineamide synthetase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,689,6130AG96.2% 587.9 / 9.6 185intergenic (‑432/+63)yfhK/purLputative 2‑component sensor protein/phosphoribosylformyl‑glycineamide synthetase
Reads supporting (aligned to +/- strand):  ref base A (4/3);  new base G (114/64);  total (118/67)
Fisher's exact test for biased strand distribution p-value = 7.05e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.05e-01

Only 100 of 196 total aligned reads displayed.

AAATCAATGAGTTAAGTGTCGCCAGAAAGCGACACGGCAAACCACCCATTGTCGTGATTTACAGACACAAAAAAGCTCCCGGAGTTGGGAGCTTATGATAGTGGTTGGTGCTTAATGCCGCATCCGGGCTGCAAAACCAATGGGCTGACGACTTACCCCAACTGCTTACGCGCATTGCG  >  NC_000913/2689524‑2689702
                                                                                         |                                                                                         
aaaTCAATGAGTTAAGTGTCGCCAGAAAGCGACACGGCAAACCACCCATTGTCGTGATTTACAGACACAAAAAAGCTCCCGGAGTTGGGGGCt                                                                                        >  1:6070646/1‑93 (MQ=255)
aaaTCAATGAGTTAAGTGTCGCCAGAAAGCGACACGGCAAACCACCCATTGTCGTGATTTACAGACACAAAAAAGCTCCCGGAGTTGGGGGCt                                                                                        >  2:5397525/1‑93 (MQ=255)
   tCAATGAGTTAAGTGTCGCCAGAAAGCGACACGGCAAACCACCCATTGTCGTGATTTACAGACACAAAAAAGCTCCCGGAGTTGGGGGCTTAt                                                                                     >  2:1492209/1‑93 (MQ=255)
   tCAATGAGTTAAGTGTCGCCAGAAAGCGACACGGCAAACCACCCATTGTCGTGATTTACAGACACAAAAAAGCTCCCGGAGTTGGGGGCTTAt                                                                                     <  1:1339990/93‑1 (MQ=255)
   tCAATGAGTTAAGTGTCGCCAGAAAGCGACACGGCAAACCACCCATTGTCGTGATTTACAGACACAAAAAAGCTCCCGGAGTTGGGGGCTTAt                                                                                     >  2:3949030/1‑93 (MQ=255)
   tCAATGAGTTAAGTGTCGCCAGAAAGCGACACGGCAAACCACCCATTGTCGTGATTTACAGACACAAAAAAGCTCCCGGAGTTGGGGGCTTAt                                                                                     >  2:5695097/1‑93 (MQ=255)
   tCAATGAGTTAAGTGTCGCCAGAAAGCGACACGGCAAACCACCCATTGTCGTGATTTACAGACACAAAAAAGCTCCCGGAGTTGGGAGCTTAt                                                                                     <  2:1849462/93‑1 (MQ=255)
   acAATGAGTTAAGTGTCGCCAGAAAGCGACACGGCAAACCACCCATTGTCGTGATTTACAGACACAAAAAAGCTCCCGGAGTTGGGGGCTTAt                                                                                     <  2:2675841/92‑1 (MQ=255)
    cAATGAGTTAAGTGTCGCCAGAAAGCGACACGGCAAACCACCCATTGTCGTGATTTACAGACACAAAAAAGCTCCCGGAGCTGGGGGCTTATg                                                                                    >  2:2304720/1‑93 (MQ=255)
     aaTGAGTTAAGTGTCGCCAGAAAGCGACACGGCAAACCACCCATTGTCGTGATTTATAGACACAAAAAAGCTCCCGGAGTTGGGGGCTTATgt                                                                                   >  2:3939648/1‑92 (MQ=255)
     aaTGAGTTAAGTGTCGCCAGAAAGCGACACGGCAAACCACCCATTGTCGTGATTTACAGACACAAAAAAGCTCCCGGAGTTGGGAGCTTATGa                                                                                   <  2:4161500/93‑1 (MQ=255)
     aaTGAGTTAAGTGTCGCCAGAAAGCGACACGGCAAACCACCCATTGTCGTGATTTACAGACACAAAAAAGCTCCCGGAGATGGGGGATTATgt                                                                                   >  1:4123765/1‑92 (MQ=255)
      aTGAGTTAAGTGTCGCCAGAAAGCGACACGGCAAACCACCCATTGTCGTGATTTACAGACACAAAAAAGCTCCCGGAGTTGGGGGCTTATGAt                                                                                  >  1:3140841/1‑93 (MQ=255)
      aTGAGTTAAGTGTCGCCAGAAAGCGACACGGCAAACCACCCATTGTCGTGATTTACAGACACAAAAAAGCTCCCGGAGTTGGGGGCTTATGAt                                                                                  >  1:2540525/1‑93 (MQ=255)
      aTGAGTTAAGTGTCGCCAGAAAGCGACACGGCAAACCACCCATTGTCGTGATTTACAGACACAAAAAAGCTCCCGGAGTTGGGGGCTTATGAt                                                                                  >  2:852382/1‑93 (MQ=255)
       tGAGTTAAGTGTCGCCAGAAAGCGACACGGCAAACCACCCATTGTCGTGATTTACAGACACAAAAAAGCTCCCGGAGTTGGGGGCTTATGATa                                                                                 >  2:3209500/1‑93 (MQ=255)
        gAGTTAAGTGTCGCCAGAAAGCGACACGGCAAACCACCCATTGTCGTGATTTACAGACACAAAAAAGCTCCCGGAGTTGGGGGCTTATGATAg                                                                                >  2:1684917/1‑93 (MQ=255)
        gAGTTAAGTGTCGCCAGAAAGCGACACGGCAAACCACCCATTGTCGTGATTTACAGACACAAAAAAGCTCCCGGAGTTGGGGGCTTATGATAg                                                                                >  1:4972491/1‑93 (MQ=255)
        gAGTTAAGTGTCGCCAGAAAGCGACACGGCAAACCACCCATTGTCGTGATTTACAGACACAAAAAAGCTCCCGGAGTTGGGGGCTTATGATAg                                                                                >  1:2845405/1‑93 (MQ=255)
         aGTTAAGTGTCGCCAGAAAGCGACACGGCAAACCACCCATTGTCGTGATTTACAGACACAAAAAAGCTCCCGGAGTTGGGGGCTTATGATAGt                                                                               <  1:4715701/93‑1 (MQ=255)
          gTTAAGTGTCGCCAGAAAGCGACACGGCAAACCACCCATTGTCGTGATTTACAGACACAAAAAAGCTCCCGGAGTTGGGGGCTTATGATAGtg                                                                              <  2:4951387/93‑1 (MQ=255)
           ttAAGTGTCGCCAGAAAGCGACACGGCAAACCACCCATTGTCGTGATTTACAGACACAAAAAAGCTCCCGGAGTTGGGGGCTTATGATAGtgg                                                                             >  2:5234731/1‑93 (MQ=255)
            tAAGTGTCGCCAGAAAGCGACACGGCAAACCACCCATTGTCGTGATTTACAGACACAAAAAAGCTCCCGGAGTTGGGGGCTTATGATAGtggt                                                                            >  1:6002185/1‑93 (MQ=255)
             aaGTGTCGCCAGAAAGCTACACGGCAAACCACCCATTGTCGTGATTTACAGACACAAAAAAGCTCCCGGAGTTGGGGGCTTATGATAGtggtt                                                                           <  1:730339/93‑1 (MQ=255)
             aaGTGTCGCCAGAAAGCGACACGGCAAACCACCCATTGTCGTGATTTACAGACACAAAAAAGCTCGCGGAGTTGGGGGCTTATGATAGtggtt                                                                           >  1:2572189/1‑93 (MQ=255)
             aaGTGTCGCCAGAAAGCGACACGGCAAACCACCCATTGTCGTGATTTACAGACACAAAAAAGCTCCCGGAGTTGGGAGCTTATGATAGtggtt                                                                           >  1:1040929/1‑93 (MQ=255)
              aGTGTCGCCAGAAAGCGACACGGCAAACCACCCATTGTCGTGATTTACAGACACAAAAAAGCTCCCGGAGTTGGGGGCTTATGATAGtggttg                                                                          >  1:3063674/1‑93 (MQ=255)
               gtgtCGCCAGAAAGCGACACGGCAAACCACCCATTGTCGTGATTTACAGACACAAAAAAGCTCCCGGAGTTGGGGGCTTATGATAGtggttgg                                                                         <  2:2127979/93‑1 (MQ=255)
               gtgtCGCCAGAAAGCGACACGGCAAACCACCCATTGTCGTGATTTACAGACACAAAAAAGCTCCCGGAGTTGGGGGCTTATGATAGtggttgg                                                                         >  2:2555788/1‑93 (MQ=255)
                 gtCGCCAGAAAGCGACACGGCAAACCACCCATTGTCGTGATTTACAGACACAAAAAAGCTCCCGGAGTTGGGGGCTTATGATAGTGGTTGGTg                                                                       >  1:836335/1‑93 (MQ=255)
                 gtCGCCAGAAAGCGACACGGCAAACCACCCATTGTCGTGATTTACAGACACAAAAAAGCTCCCGGAGTTGGGGGCTTATGATAGTGGTTGGTg                                                                       >  2:4862825/1‑93 (MQ=255)
                  tCGCCAGAAAGCGACACGGCAAACCACCCATTGTCGTGATTTACAGACACAAAAAAGCTCCTGGAGTTGGGGGCTTATGATAGTGGTTGGTGc                                                                      >  2:3890447/1‑93 (MQ=255)
                     ccAGAAAGCGACACGGCAAACCACCCATTGTCGTGATTTACAGACACAAAAAAGCTCCCGGAGTTGGGGGCTTATGATAGTGGTTGGTGCTTa                                                                   >  1:2123803/1‑93 (MQ=255)
                     ccAGAAAGCGACACGGCAAACCACCCATTGTCGTGATTTACAGACACAAAAAAGCTCCCGGAGTTGGGGGCTTATGATAGTGGTTGGTGCTTa                                                                   <  1:1871888/93‑1 (MQ=255)
                     ccAGAAAGCGACACGGCAAACCACCCATTGTCGTGATTTACAGACACAAAAAAGCTCCCGGAGTTGGGGGCTTATGATAGTGGTTGGTGCTTa                                                                   >  1:2247658/1‑93 (MQ=255)
                      cAGAAAGCGACACGGCAAACCACCCATTGTCGTGATTTACAGACACAAAAAAGCTCCCGGAGTTGGGGGCTTATGATAGTGGTTGGTGCTTaa                                                                  <  2:1310543/93‑1 (MQ=255)
                      cAGAAAGCGACACGGCAAACCACCCATTGTCGTGATTTACAGACACAAAAAAGCTCCCGGAGTTGGGGGCTTATGATAGTGGTTGGTGCTTaa                                                                  >  1:6109561/1‑93 (MQ=255)
                        gAAAGCGACACGGCAAACCACCCATTGTCGTGATTTACAGACACAAAAAAGCTCCCGGAGTTGGGGGCTTATGATAGTGGTTGGTGCTTAATg                                                                >  1:5242909/1‑93 (MQ=255)
                        gAAAGCGACACGGCAAACCACCCATTGTCGTGATTTACAGACACAAAAAAGCTCCCGGAGTTGGGGGCTTATGATAGTGGTTGGTGCTTAATg                                                                >  2:2627765/1‑93 (MQ=255)
                        gAAAGCGACACGGCAAACCACCCATTGTCGTGATTTACAGACACAAAAAAGCTCCCGGAGTTGGGGGCTTATGATAGTGGTTGGTGCTTAATg                                                                >  1:6335269/1‑93 (MQ=255)
                        gAAAGCGACACGGCAAACCACCCATTGTCGTGATTTACAGACACAAAAAAGCTCCCGGAGTTGGGGGCTTATGATAGTGGTTGGTGCTTAATg                                                                <  2:5992074/93‑1 (MQ=255)
                          aaGCGACACGGCAAACCACCCATTGTCGTGATTTACAGACACAAAAAAGCTCCCGGAGTTGGGGGCTTATGATAGTGGTTGGTGCTTAATGcc                                                              >  1:5865607/1‑93 (MQ=255)
                           aGCGACACGGCAAACCACCCATTGTCGTGATTTACAGACACAAAAAAGCTCCCGGAGTTGGGGGCTTATGATAGTGGTTGGTGCTTAATGCCg                                                             >  2:3415850/1‑93 (MQ=255)
                            gCGACACGGCAAACCACCCATTGTCGTGATTTACAGACAC‑AAAAAGCTCCCGGAGTTGGGGGCTTATGATAGTGGTTGGTGCTTAATGCCGCa                                                           >  1:6392839/1‑93 (MQ=255)
                              gACACGGCAAACCACCCATTGTCGTGATTTACAGACACAAAAAAGCTCCCGGAGTTGGGGGCTTATGATAGTGGTTGGTGCTTAATGCCGCAt                                                          >  1:3231982/1‑93 (MQ=255)
                              gACACGGCAAACCACCCATTGTCGTGATTTACAGACACAAAAAAGCTCCCGGAGTTGGGGGCTTATGATAGTGGTTGGTGCTTAATGCCGCAt                                                          >  2:1027531/1‑93 (MQ=255)
                                cacGGCAAACCACCCATTGTCGTGATTTACAGACACAAAAAAGCTCCCGGAGTTGGGGGCTTATGATAGTGGTTGGTGCTTAATGCCGCATcc                                                        <  2:5685918/93‑1 (MQ=255)
                                 acGGCAAACCACCCATTGTCGTGATTTACAGACACAAAAAAGCTCCCGGAGTTGGGGGCTTATGATAGTGGTTGGTGCTTAATGCCGCATCCg                                                       <  2:1374224/93‑1 (MQ=255)
                                 acGGCAAACCACCCATTGTCGTGATTTACAGACACAAAAAAGCTCCCGGAGTTGGGGGCTTATGATAGTGGTTGGTGCTTAATGCCGCATCCg                                                       <  1:255518/93‑1 (MQ=255)
                                 acGGCAAACCACCCATTGTCGTGATTTACAGACACAAAAAAGCTCCCGGAGTTGGGGGCTTATGATAGTGGTTGGTGCTTAATGCCGCATCCg                                                       <  2:1954032/93‑1 (MQ=255)
                                       aaCCACCCATTGTCGTGATTTACAGACACAAAAAAGCTCCCGGAGTTGGGGGCTTATGATAGTGGTTGGTGCTTAATGCCGCATCCGGGCTGc                                                 >  1:2386658/1‑93 (MQ=255)
                                         ccaccCATTGTCGTGATTTACAGACACAAAAAAGCTCCCGGAGTTGGGGGCTTATGATAGTGGTTGGTGCTTAATGCCGCATCCGGGCTGCaa                                               >  2:4369653/1‑93 (MQ=255)
                                         ccaccCATTGTCGTGATTTACAGACACAAAAAAGCTCCCGGAGTTGGGGGCTTATGATAGTGGTTGGTGCTTAATGCCGCATCCGGGCTGCaa                                               >  1:2642251/1‑93 (MQ=255)
                                            cccATTGTCGTGATTTACAGACACAAAAAAGCTCCCGGAGTTGGGGGCTTATGATAGTGGTTGGTGCTTAATGCCGCATCCGGGCTGCAAAAc                                            >  2:1789365/1‑93 (MQ=255)
                                            cccATTGTCGTGATTTACAGACACAAAAAAGCTCCCGGAGTTGGGGGCTTATGATAGTGGTTGGTGCTTAATGCCGCATCCGGGCTGCAAAAc                                            >  2:4231222/1‑93 (MQ=255)
                                             ccATTGTCGTGATTTACAGACACAAAAAAGCTCCCGGAGTTGGGGGCTTATGATAGTGGTTGGTGCTTAATGCCGCATCCGGGCTGCAAAAcc                                           >  1:1786553/1‑93 (MQ=255)
                                                ttGTCGTGATTTACAGACACAAAAAAGCTCCCGGAGTTGGGGGCTTATGATAGTGGTTGGTGCTTAATGCCGCATCCGGGCTGCAAAACCAAt                                        >  2:3343622/1‑93 (MQ=255)
                                                ttGTCGTGATTTACAGACACAAAAAAGCTCCCGGAGTTGGGGGCTTATGATAGTGGTTGGTGCTTAATGCCGCATCCGGGCTGCAAAACCAAt                                        >  2:4302933/1‑93 (MQ=255)
                                                 tGTCGTGATTTACAGACACAAAAAAGCTCCCGGAGTTGGGGGCTTATGATAGTGGTTGGTGCTTAATGCCGCATCCGGGCTGCAAAACCAATg                                       >  1:4375664/1‑93 (MQ=255)
                                                  gTCGTGATTTACAGACACAAAAAAGCTCCCGGAGTTGGGGGCTTATGATAGTGGTTGGTGCTTAATGCCGCATCCGGGCTGCAAAACCAATgg                                      >  2:5521608/1‑93 (MQ=255)
                                                  gTCGTGATTTACAGACACAAAAAAGCTCCCGGAGTTGGGGGCTTATGATAGTGGTTGGTGCTTAATGCCGCATCCGGGCTGCAAAACCAATgg                                      >  1:5332072/1‑93 (MQ=255)
                                                   tCGTGATTTACAGACACAAAAAAGCTCCCGGAGTTGGGGGCTTATGATAGTGGTTGGTGCTTAATGCCGCATCACGGCTGCAAAACCAATggg                                     >  2:1150645/1‑93 (MQ=255)
                                                    cGTGATTTACAGACACAAAAAAGCTCCCGGAGTTGGGGGCTTATGATAGTGGTTGGTGCTTAATGCCGCATCCGGGCTGCAAAACCAATGGGc                                    <  2:2999387/93‑1 (MQ=255)
                                                      tGATTTACAGACACAAAAAAGCTCCCGGAGTTGGGGGCTTATGATAGTGGTTGGTGCTTAATGCCGCATCCGGGCTGCAAAACCAATGGGCTg                                  >  1:388514/1‑93 (MQ=255)
                                                       gATTTACAGACACAAAAAAGCTCCCGGAGTTGGGGGCTTATGATAGTGGTTGGTGCTTAATGCCGCATCCGGGCTGCAAAACCAATGGGCTga                                 <  1:458878/93‑1 (MQ=255)
                                                       gATTTACAGACACAAAAAAGCTCCCGGAGTTGGGGGCTTATGATAGTGGTTGGTGCTTAATGCCGCATCCGGGCTGCAAAACCAATGGGCTga                                 <  1:1233431/93‑1 (MQ=255)
                                                        aTTTACAGACACAAAAAAGCTCCCGGAGTTGGGGGCTTATGATAGTGGTTGGTGCTTAATGCCGCATCCGGGCTGCACAACCAATGGGCTgcc                                >  2:2334322/1‑91 (MQ=255)
                                                        aTTTACAGACACAAAAAAGCTCCCGGAGTTGGGGGCTTATGATAGTGGTTGGTGCTTAATGCCGCATCCGGGCTGCAAAACCAATGGGCTgac                                >  1:3191400/1‑93 (MQ=255)
                                                        aTTTACAGACACAAAAAAGCTCCCGGAGTTGGGGGCTTATGATAGTGGTTGGTGCTTAATGCCGCATCCGGGCTGCAAAACCAATGGGCTgac                                >  2:4676239/1‑93 (MQ=255)
                                                         tttAGAGACACAAAAAAGCTCCCGGAGTTGGGGGCTTATGATAGTGGTTGGTGCTTAATGCCGCATCCGGGCTGCAAAACCAATGGGCTgacg                               <  2:6257430/93‑1 (MQ=255)
                                                         tttACAGACACAAAAAAGCTCCCGGAGTTGGGGGCTTATGATAGTGGTTGGTGCTTAATGCCGCATCCGGGCTGCAAAACCAATGGGCTgacg                               >  1:4358977/1‑93 (MQ=255)
                                                         tttACAGACACAAAAAAGCTCCCGGAGTTGGGGGCTTATGATAGTGGTTGGTGCTTAATGCCGCATCCGGGCTGCAAAACCAATGGGCTgacg                               >  1:6414037/1‑93 (MQ=255)
                                                          ttACAGACACAAAAAAGCTCCCGGAGTTGGGGGCTTATGATAGTGGTTGGTGCTTAATGCCGCATCCGGGCTGCAAAACCAATGGGCTgacga                              >  2:3257829/1‑93 (MQ=255)
                                                              aGACACAAAAAAGCTCCCGGAGTTGGGGGCTTATGATAGTGGTTGGTGCTTAATGCCGCATCCGGGCTGCAAAACCAATGGGCTGACGACTTa                          <  1:2658982/93‑1 (MQ=255)
                                                                acacAAAAAAGCTCCGGGGGTGGGGGGCTTATGATAGTGGTTGGTGTTAAAGGCCGCACCCGGCCTGAAAACCAAATGGGCTGACGCCTTAcc                        <  2:5174059/93‑1 (MQ=255)
                                                                acacAAAAAAGCTCCCGGAGTTGGGGGCTTATGATAGTGGTTGGTGCTTAATGCCGCATCCGGGCTGCAAAACCAATGGGCTGACGACTTAcc                        <  2:4522912/93‑1 (MQ=255)
                                                                 cacaAAAAAGCTCCCGGAGTTGGGGGCTTATGATAGTGGTTGGTGCTTAATGCCGCATCCGGGCTGCAAAACCAATGGGCTGACGACTTAccc                       <  2:6040182/93‑1 (MQ=255)
                                                                   caAAAAAGTTCCCGGAGTTGGGGGCTTATGATAGTGGTTGGTGCTTAATGCCGCATCCGGGCTGCAAAACCAATGGGCTGACGACTTACCCCa                     >  2:6313185/1‑93 (MQ=255)
                                                                   caAAAAAGCTCCCGGAGTTGGGGGCTTATGATAGTGGTTGGTGCTTAATGCCGCATCCGGGCTGCAAAACCAATGGGCTGACGACTTACCCCa                     >  1:5824607/1‑93 (MQ=255)
                                                                    aaaaaaGCTCCCGGAGTTGGGGGCTTATGATAGTGGTTGGTGCTTAATGCCGCATCCGGGCTGCAAAACCAATGGGCTGACGACTTACCCCaa                    <  1:575532/93‑1 (MQ=255)
                                                                     aaaaaGCTCCCGGAGTTGGGGGCTTATGATAGTGGTTGGTGCTTAATGCCGCATCCGGGCTGCAAAACCAATGGGCTGACGACTTACCCCAAc                   >  2:3657975/1‑93 (MQ=255)
                                                                        aaGCTCCCGGAGTTGGGGGCTTATGATAGTGGTTGGTGCTTAATGCCGCATCCGGGCTGCAAAACCAATGGGCTGACGACTTACCCCAACTGc                >  1:2978336/1‑93 (MQ=255)
                                                                        aaGCTCCCGGAGTTGGGGGCTTATGATAGTGGTTGGTGCTTAATGCCGCATCCGGGCTGCAAAACCAATGGGCTGACGACTTACCCCAACTGc                <  2:4467719/93‑1 (MQ=255)
                                                                         aGCTCCCGGAGTTGGGGGCTTATGATAGTGGTTGGTGCTTAATGCCGCATCCGGGCTGCAAAACCAATGGGCTGACGACTTACCCCAACTGCt               >  2:6473573/1‑93 (MQ=255)
                                                                          gCTCCCGGAGTTGGGGGCTTATGATAGTGGTTGGTGCTTAATGCCGCATCCGGGCTGCAAAACCAATGGGCTGACGACTTACCCCAACTGCtt              <  2:886703/93‑1 (MQ=255)
                                                                            tCCCGGAGTTGGGGGCTTATGATAGTGGTTGGTGCTTAATGCCGCATCCGGGCTGCAAAACCAATGGGCTGACGACTTACCCCAACTGCTTAc            >  2:1739297/1‑93 (MQ=255)
                                                                            tCCCGGAGTTGGGGGCTTATGATAGTGGTTGGTGCTTAATGCCGCATCCGGGCTGCAAAACCAATGGGCTGACGACTTACCCCAACTGCTTAc            >  2:1567211/1‑93 (MQ=255)
                                                                              ccGGAGTTGGGGGCTTATGATAGTGGTTGGTGCTTAATGCCGCATCCGGGCTGCAAAACCAATGGGCTGACGACTTACCCCAACTGCTTAcgc          >  2:4543206/1‑93 (MQ=255)
                                                                              ccGGAGTTGGGGGCTTATGATAGTGGTTGGTGCTTAATGCCGCATCCGGGCTGCAAAACCAATGGGCTGACGACTTACCCCAACTGCTTAcgc          <  1:4212562/93‑1 (MQ=255)
                                                                                 gAGTTGGGGGCTTATGATAGTGGTTGGTGCTTAATGCCGCATCCGGGCTGCAAAACCAATGGGCTGACGACTTACCCCAACTGCTTACGCGCa       >  1:6146405/1‑93 (MQ=255)
                                                                                 gAGTTGGGGGCTTATGATAGTGGTTGGTGCTTAATGCCGCATCCGGGCTGCAAAACCAATGGGCTGACGACTTACCCCAACTGCTTACGCGCa       >  1:1523995/1‑93 (MQ=255)
                                                                                 gAGTTGGGGGCTTATGATAGTGGTTGGTGCTTAATGCCGCATCCGGGCTGCAAAACCAATGGGCTGACGACTTACCCCAACTGCTTACGCGCa       <  1:1205040/93‑1 (MQ=255)
                                                                                 gAGTTGGGGGCTTATGATAGTGGTTGGGGCTTAATGCCGCATCCGGGCTGCAAAACCAATGGGCTGACGACTTACCCCAACTGCTTACGCGCa       >  2:2755725/1‑93 (MQ=255)
                                                                                  aGTTGGGGGCTTATGATAGTGGTTGGTGCTTAATGCCGCATCCGGGCTGCAAAACCAATGGGCTGACGACTTACCCCAACTGCTTACGCGCAt      >  1:4655238/1‑93 (MQ=255)
                                                                                  aGTTGGGAGCTTATGATAGTGGTTGGTGCTTAATGCCGCATCCGGGCTGCAAAACCAATGGGCTGACGACTTACCCCAACTGCTTACGCGCAt      <  2:2583311/93‑1 (MQ=255)
                                                                                   gTTGGGGGCTTATGATAGTGGTTGGTGCTTAATGCCGCATCCGGGCTGCAAAACCAATGGGCTGACGACTTACCCCAACTGCTTACGCGCAtt     >  2:3596038/1‑93 (MQ=255)
                                                                                    gtGGGGGCTTATGATAGTGGTTGGTGCTTAATGCCGCATCCGGGCTGCAAAACCAATGGGCTGACGACTTACCCCAACTGCTTACGCGCATTg    <  1:2428045/92‑1 (MQ=255)
                                                                                     tGGGGGCTTATGATAGTGGTTGGTGCTTAATGCCGCATCCGGGCTGCAAAACCAATGGGCTGACGACTTACCCCAACTGCTTACGCGCATTGc   >  2:5042724/1‑93 (MQ=255)
                                                                                     tGGGGGCTTATGATAGTGGTTGGTGCTTAATGCCGCATCCGGGCTGCAAAACCAATGGGCTGACGACTTACCCCAACTGCTTACGCGCATTGc   <  2:4038694/93‑1 (MQ=255)
                                                                                      ggggGCTTATGATAGTGGTTGGTGCTTAATGCCGCATCCGGGCTGCAAAACCAATGGGCTGACGACTTACCCCAACTGCTTACGCGCATTGCg  <  1:4821160/93‑1 (MQ=255)
                                                                                         |                                                                                         
AAATCAATGAGTTAAGTGTCGCCAGAAAGCGACACGGCAAACCACCCATTGTCGTGATTTACAGACACAAAAAAGCTCCCGGAGTTGGGAGCTTATGATAGTGGTTGGTGCTTAATGCCGCATCCGGGCTGCAAAACCAATGGGCTGACGACTTACCCCAACTGCTTACGCGCATTGCG  >  NC_000913/2689524‑2689702

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
AAAATCAATGAGTTAAGTGTCGCCAGAAAGCGACACGGCAAACCACCCATTGTCGTGATTTACAGACACAAAAAAGCTCCCGGAGTTGGGAGCTTATGATAGTGGTTGGTGCTTAATGCCGCATCCGGGCTGCAAAACCAATGGGCTGACGACTTACCCCAACTGCTTACGCGCATTGCGGA  >  NC_000913/2689523‑2689704
                                                                                          |                                                                                           
AAAATCAATGAGTTAAGTGTCGCCAGAAAGCGACACGGCAAACCACCCATTGTCGTGATTTACAGACACAAAAAAGCTCCCGGAGTTGGGGGCT                                                                                          >  SRR4302537.5866878/1‑94 (MQ=60)
AAAATCAATGAGTTAAGTGTCGCCAGAAAGCGACACGGCAAACCACCCATTGTCGTGATTTACAGACACAAAAAAGCTCCCGGAGTTGGGGGCT                                                                                          >  SRR4302537.6112355/1‑94 (MQ=60)
 AAATCAATGAGTTAAGTGTCGCCAGAAAGCGACACGGCAAACCACCCATTGTCGTGATTTACAGACACAAAAAAGCTCCCGGAGTTGGGGGCTT                                                                                         >  SRR4302537.6422188/1‑94 (MQ=60)
 AAATCAATGAGTTAAGTGTCGCCAGAAAGCGACACGGCAAACCACCCATTGTCGTGATTTACAGACACAAAAAAGCTCCCGGAGTTGGGGGCTT                                                                                         >  SRR4302537.3081150/1‑94 (MQ=60)
   ataaaggatTTAAGTGTCGCAAGAAAGCGCCACGGCAAACCACCCATTGTCGTGATTTACAGACACAAAAAAGCTCCCGGAGTTGGGGGCTTAT                                                                                       <  SRR4302537.5530964/85‑1 (MQ=60)
   ATCAATGAGTTAAGTGTCGCCAGAAAGCGACACGGCAAACCACCCATTGTCGTGATTTACAGACACAAAAAAGCTCCCGGAGTTGGGGGCTTAT                                                                                       >  SRR4302537.2869139/1‑94 (MQ=60)
    TCAATGAGTTAAGTGTCGCCAGAAAGCGACACGGCAAACCACCCATTGTCGTGATTTACAGACACAAAAAAGCTCCCGGAGTTGGGGGCTTATG                                                                                      <  SRR4302537.1349322/94‑1 (MQ=60)
    TCAATGAGTTAAGTGTCGCCAGAAAGCGACACGGCAAACCACCCATTGTCGTGATTTACAGACACAAAAAAGCTCCCGGAGTTGGGGGCTTATG                                                                                      <  SRR4302537.3136983/94‑1 (MQ=60)
     CAATGAGTTAAGTGTCGCCAGAAAGCGACACGGCAAACCA‑CCATTGTCGTGGTTTACAGACAACAAAAAGCTCCCGGAGTTGGGGGCTTATGAT                                                                                    >  SRR4302537.2673116/1‑94 (MQ=60)
      AATGAGTTAAGTGTCGCCAGAAAGCGACACGGCAAACCACCCATTGTCGTGATTTACAGACACAAAAAAGCTCCCGGAGTTGGGGGCTTATGAT                                                                                    >  SRR4302537.3162883/1‑94 (MQ=60)
      AATGAGTTAAGTGTCGCCAGAAAGCGACACGGCAAACCACCCATTGTCGTGATTTACAGACACAAAAAAGCTCCCGGAGTTGGGGGCTTATGAT                                                                                    >  SRR4302537.2558339/1‑94 (MQ=60)
        TGAGTTAAGTGTCGCCAGAAAGCGACACGGCAAACCACCCATTGTCGTGATTTACAGACACAAAAAAGCTCCCGGAGTTGGGGGCTTATGATAG                                                                                  >  SRR4302537.5006763/1‑94 (MQ=60)
        TGAGTTAAGTGTCGCCAGAAAGCGACACGGCAAACCACCCATTGTCGTGATTTACAGACACAAAAAAGCTCCCGGAGTTGGGGGCTTATGATAG                                                                                  >  SRR4302537.2865324/1‑94 (MQ=60)
          AGTTAAGTGTCGCCAGAAAGCGACACGGCAAACCACCCATTGTCGTGATTTACAGACACAAAAAAGCTCCCGGAGTTGGGGGCTTATGATAGTG                                                                                <  SRR4302537.1887452/94‑1 (MQ=60)
          AGTTAAGTGTCGCCAGAAAGCGACACGGCAAACCACCCATTGTCGTGATTTACAGACACAAAAAAGCTCCCGGAGTTGGGGGCTTATGATAGTG                                                                                <  SRR4302537.4748315/94‑1 (MQ=60)
           GTAAAGTGTCGCCAGAAAGCGACACGGCAAACCACCCATTGTCGTGATTTACAGACACAAAAAAGCTCCCGGAGTTGGGGGCTTATGATAGTGG                                                                               <  SRR4302537.4782488/94‑1 (MQ=60)
            TTAAGTGTCGCCAGAAAGCGACACGGCAAACCACCCATTGTCGTGATTTACAGACACAAAAAAGCTCCCGGAGTTGGGGGCTTATGATAGTGGT                                                                              >  SRR4302537.6043416/1‑94 (MQ=60)
            TTAAGTGTCGCCAGAAAGCGACACGGCAAACCACCCATTGTCGTGATTTACAGACACAAAAAAGCTCCCGGAGTTGGGGGCTTATGATAGTGGT                                                                              <  SRR4302537.6115758/94‑1 (MQ=60)
            TTAAGTGTCGCCAGAAAGCGACACGGCAAACCACCCATTGTCGTGATTTACAGACACAAAAAAGCTCCCGGAGTTGGGGGCTTATGATAGTGGT                                                                              >  SRR4302537.5203811/1‑94 (MQ=60)
             TAAGTGTCGCCAGAAAGCGACACGGCAAACCACCCATTGTCGTGATTTACAGACACAAAAAAGCTCGCGGAGTTGGGGGCTTATGATAGTGGTT                                                                             >  SRR4302537.2590203/1‑94 (MQ=60)
             TAAGTGTCGCCAGAAAGCGACACGGCAAACCACCCATTGTCGTGATTTACAGACACAAAAAAGCTCCCGGAGTTGGGAGCTTATGATAGTGGTT                                                                             >  SRR4302537.1048120/1‑94 (MQ=60)
              AAGTGTCGCCAGAAAGCTACACGGCAAACCACCCATTGTCGTGATTTACAGACACAAAAAAGCTCCCGGAGTTGGGGGCTTATGATAGTGGTTG                                                                            <  SRR4302537.735326/94‑1 (MQ=60)
              AAGTGTCGCCAGAAAGCGACACGGCAAACCACCCATTGTCGTGATTTACAGACACAAAAAAGCTCCCGGAGTTGGGGGCTTATGATAGTGGTTG                                                                            >  SRR4302537.3085017/1‑94 (MQ=60)
               AGTGTCGCCAGAAAGCGACACGGCAAACCACCCATTGTCGTGATTTACAGACACAAAAAAGCTCCCGGAGTTGGGGGCTTATGATAGTGGTTGG                                                                           >  SRR4302537.1706064/1‑94 (MQ=60)
                 TGTCGCCAGAAAGCGACACGGCAAACCACCCATTGTCGTGATTTACAGACACAAAAAAGCTCCCGGAGTTGGGGGCTTATGATAGTGGTTGGTG                                                                         >  SRR4302537.842061/1‑94 (MQ=60)
                  GTCGCCAGAAAGCGACACGGCAAACCACCCATTGTCGTGATTTACAGACACAAAAAAGCTCCCGGAGTTGGTGGCTTATGATAGTGGGTGGGGC                                                                        >  SRR4302537.3226904/1‑94 (MQ=60)
                     GCCAGAAAGCGACACGGCAAACCACCCATTGTCGTGATTTACAGACACAAAAAAGCTCCCGGAGTTGGGGGCTTATGATAGTGGTTGGTGCTTA                                                                     >  SRR4302537.2138745/1‑94 (MQ=60)
                     GCCAGAAAGCGACACGGCAAACCACCCATTGTCGTGATTTACAGACACAAAAAAGCTCCCGGAGTTGGGGGCTTATGATAGTGGTTGGTGCTTA                                                                     >  SRR4302537.2263519/1‑94 (MQ=60)
                      CCAGAAAGCGACACGGCAAACCACCCATTGTCGTGATTTACAGACACAAAAAAGCTCCCGGAGTTGGGGGCTTATGATAGTGGTTGGTGCTTAA                                                                    >  SRR4302537.6151548/1‑94 (MQ=60)
                      CCAGAAAGCGACACGGCAAACCACCCATTGTCGTGATTTACAGACACAAAAAAGCTCCCGGAGTTGGGGGCTTATGATAGTGGTTGGTGCTTAA                                                                    <  SRR4302537.1884915/94‑1 (MQ=60)
                        AGAAAGCGACACGGCAAACCACCCATTGTCGTGATTTACAGACACAAAAAAGCTCCCGGAGTTGGGGGCTTATGATAGTGGTTGGTGCTTAATG                                                                  >  SRR4302537.5278990/1‑94 (MQ=60)
                        AGAAAGCGACACGGCAAACCACCCATTGTCGTGATTTACAGACACAAAAAAGCTCCCGGAGTTGGGGGCTTATGATAGTGGTTGGTGCTTAATG                                                                  >  SRR4302537.6378578/1‑94 (MQ=60)
                        AGAAAGCGACACGGCAAACCACCCATTGTCGTGATTTACAGACACAAAAAAGCTCCCGGAGTTGGGAGCTTATGATAGTGGTTGGTGCTTAATG                                                                  >  SRR4302537.5815216/1‑94 (MQ=60)
                          AAAGCGACACGGCAAACCACCCATTGTCGTGATTTACAGACACAAAAAAGCTCCCGGAGTTGGGGGCTTATGATAGTGGTTGGTGCTTAATGCC                                                                >  SRR4302537.5905846/1‑94 (MQ=60)
                            AGCGACACGGCAAACCACCCATTGTCGTGATTTACAGACACAAAAAAGCTCCCGGAGTTGGGGGCTTATGATAGTGGTTGGTGCTTAATGCCGC                                                              >  SRR4302537.742651/1‑94 (MQ=60)
                            AGCGACACGGCAAACCACCCATTGTCGTGATTTACAGACAC‑AAAAAGCTCCCGGAGTTGGGGGCTTATGATAGTGGTTGGTGCTTAATGCCGCA                                                             >  SRR4302537.6436385/1‑94 (MQ=60)
                              CGACACGGCAAACCACCCATTGTCGTGATTTACAGACACAAAAAAGCTCCCGGAGTTGGGGGCTTATGATAGTGGTTGGTGCTTAATGCCGCAT                                                            >  SRR4302537.2597200/1‑94 (MQ=60)
                              CGACACGGCAAACCACCCATTGTCGTGATTTACAGACACAAAAAAGCTCCCGGAGTTGGGGGCTTATGATAGTGGTTGGTGCTTAATGCCGCAT                                                            >  SRR4302537.3254757/1‑94 (MQ=60)
                                ACACGGCAAACCACCCATTGTCGTGATTTACAGACACAAAAAAGCTCCCGGAGTTGGGGGCTTATGATAGTGGTTGGTGCTTAATGCCGCATCC                                                          <  SRR4302537.5346924/94‑1 (MQ=60)
                                ACACGGCAAACCACCCATTGTCGTGATTTACAGACACAAAAAAGCTCCCGGAGTTGGGGGCTTATGATAGTGGTTGGTGCTTAATGCCGCATCC                                                          >  SRR4302537.4140595/1‑94 (MQ=60)
                                 CACGGCAAACCACCCATTGTCGTGATTTACAGACACAAAAAAGCTCCCGGAGTTGGGGGCTTATGATAGTGGTTGGTGCTTAATGCCGCATCCG                                                         <  SRR4302537.6093244/94‑1 (MQ=60)
                                 CACGGCAAACCACCCATTGTCGTGATTTACAGACACAAAAAAGCTCCCGGAGTTGGGGGCTTATGATAGTGGTTGGTGCTTAATGCCGCATCCG                                                         >  SRR4302537.3167867/1‑94 (MQ=60)
                                  ACGGCAAACCACCCATTGTCGTGATTTACAGACACAAAAAAGCTCCCGGAGTTGGGGGCTTATGATAGTGGTTGGTGCTTAATGCCGCATCCGG                                                        <  SRR4302537.257329/94‑1 (MQ=60)
                                   CGGCAAACCACCCATTGTCGTGATTTACAGACACAAAAAAGCTCCCGGAGTTGGGGGCTTATGATAGTGGTTGGTGCTTAATGCCGCATCCGGG                                                       <  SRR4302537.3702349/94‑1 (MQ=60)
                                       AAACCACCCATTGTCGTGATTTACAGACACAAAAAAGCTCCCGGAGTTGGGGGCTTATGATAGTGGTTGGTGCTTAATGCCGCATCCGGGCTGC                                                   >  SRR4302537.2403467/1‑94 (MQ=60)
                                         ACCACCCATTGTCGTGATTTACAGACACAAAAAAGCTCCCGGAGTTGGGGGCTTATGATAGTGGTTGGTGCTTAATGCCGCATCCGGGCTGCAA                                                 >  SRR4302537.2660738/1‑94 (MQ=60)
                                         ACCACCCATTGTCGTGATTTACAGACACAAAAAAGCTCCCGGAGTTGGGGGCTTATGATAGTGGTTGGTGCTTAATGCCGCATCCGGGCTGCAA                                                 <  SRR4302537.2519029/94‑1 (MQ=60)
                                          CCACCCATTGTCGTGATTTACAGACACAAAAAAGCTCCCGGAGTTGGGGGCTTATGATAGTGGTTGGTGCTTAATGCCGCATCCGGGCTGCAAA                                                >  SRR4302537.2152893/1‑94 (MQ=60)
                                           CACCCATTGTCGTGATTTACAGACACAAAAAAGCTCCCGGAGTTGGGGGCTTATGATAGTGGTTGGTGCTTAATGCCGCATCCGGGCTGCAACA                                               >  SRR4302537.4375180/1‑94 (MQ=60)
                                            ACCCATTGTCGTGATTTACAGACACAAAAAAGCTCCCGGAGTTGGGGGCTTATGATAGTGGTTGGTGCTTAATGCCGCATCCGGGCTGCAAAAC                                              >  SRR4302537.2436766/1‑94 (MQ=60)
                                             CCCATTGTCGTGATTTACAGACACAAAAAAGCTCCCGGAGTTGGGGGCTTATGATAGTGGTTGGTGCTTAATGCCGCATCCGGGCTGCAAAACC                                             >  SRR4302537.1799012/1‑94 (MQ=60)
                                                 TTGTCGTGATTTACAGACACAAAAAAGCTCCCGGAGTTGGGGGCTTATGATAGTGGTTGGTGCTTAATGCCGCATCCGGGCTGCAAAACCAATG                                         >  SRR4302537.4405884/1‑94 (MQ=60)
                                                  TGTCGTGATTTACAGACACAAAAAAGCTCCCGGAGTTGGGGGCTTATGATAGTGGTTGGTGCTTAATGCCGCATCCGGGCTGCAAAACCAATGG                                        >  SRR4302537.5368646/1‑94 (MQ=60)
                                                  TGTCGTGATTTACAGACACAAAAAAGCTCCCGGAGTTGGGGGCTTATGATAGTGGTTGGTGCTTAATGCCGCATCCGGGCTGCAAAACCAATGG                                        <  SRR4302537.616410/94‑1 (MQ=60)
                                                      GTGATTTACAGACACAAAAAAGCTCCCGGAGTTGGGGGCTTATGATAGTGGTTGGTGCTTAATGCCGCATCCGGGCTGCAAAACCAATGGGCTG                                    >  SRR4302537.391200/1‑94 (MQ=60)
                                                        GATTTACAGACACAAAAAAGCTCCCGGAGTTGGGGGCTTATGATAGTGGTTGGTGCTTAATGCCGCATCCGGGCTGCAAAACCAATGGGCTGAC                                  <  SRR4302537.462040/94‑1 (MQ=60)
                                                        GATTTACAGACACAAAAAAGCTCCCGGAGTTGGGGGCTTATGATAGTGGTTGGTGCTTAATGCCGCATCCGGGCTGCAAAACCAATGGGCTGAC                                  >  SRR4302537.3213840/1‑94 (MQ=60)
                                                        GATTTACAGACACAAAAAAGCTCCCGGAGTTGGGGGCTTATGATAGTGGTTGGTGCTTAATGCCGCATCCGGGCTGCAAAACCAATGGGCTGAC                                  <  SRR4302537.4707911/94‑1 (MQ=60)
                                                        GATTTACAGACACAAAAAAGCTCCCGGAGTTGGGGGCTTATGATAGTGGTTGGTGCTTAATGCCGCATCCGGGCTGCAAAACCAATGGGCTGAC                                  <  SRR4302537.1242012/94‑1 (MQ=60)
                                                         ATTTACAGACACAAAAAAGCTCCCGGAGTTGGGGGCTTATGATAGTGGTTGGTGCTTAATGCCGCATCCGGGCTGCAAAACCAATGGGCTGACG                                 >  SRR4302537.6457663/1‑94 (MQ=60)
                                                         ATTTACAGACACAAAAAAGCTCCCGGAGTTGGGGGCTTATGATAGTGGTTGGTGCTTAATGCCGCATCCGGGCTGCAAAACCAATGGGCTGACG                                 <  SRR4302537.733807/94‑1 (MQ=60)
                                                         ATTTACAGACACAAAAAAGCTCCCGGAGTTGGGGGCTTATGATAGTGGTTGGTGCTTAATGCCGCATCCGGGCTGCAAAACCAATGGGCTGACG                                 >  SRR4302537.4389141/1‑94 (MQ=60)
                                                               AGACACAAAAAAGCTCCCGGAGTTGGGGGCTTATGATAGTGGTTGGTGCTTAATGCCGCATCCGGGCTGCAAAACCAATGGGCTGACGACTTAC                           <  SRR4302537.2677558/94‑1 (MQ=60)
                                                               AGACACAAAAAAGCTCCCGGAGTTGGGGGCTTATGATAGTGGTTGGTGCTTAATGCCGCATCCGGGCTGCAAAACCAATGGGCTGACGACTTAC                           <  SRR4302537.4516355/94‑1 (MQ=60)
                                                                   ACAAAAAAGCTCCCGGAGTTGGGGGCTTATGATAGTGGTTGGTGCTTAATGCCGCATCCGGGCTGCAAAACCAATGGGCTGACGACTTACCCCA                       >  SRR4302537.135176/1‑94 (MQ=60)
                                                                   ACAAAAAAGCTCCCGGAGTTGGGGGCTTATGATAGTGGTTGGTGCTTAATGCCGCATCCGGGCTGCAAAACCAATGGGCTGACGACTTACCCCA                       >  SRR4302537.5864524/1‑94 (MQ=60)
                                                                    CAAAAAAGCTCCCGGAGTTGGGGGCTTATGATAGTGGTTGGTGCTTAATGCCGCATCCGGGCTGCAAAACCAATGGGCTGACGACTTACCCCAA                      <  SRR4302537.255925/94‑1 (MQ=60)
                                                                     AAAAAAGCTCCCGGAGTTGGGGGCTTATGATAGTGGTTGGTGCTTAATGCCGCATCCGGGCTGCAAAACCAATGGGCTGACGACTTACCCCAAC                     >  SRR4302537.1834388/1‑94 (MQ=60)
                                                                     AAAAAAGCTCCCGGAGTTGGGGGCTTATGATAGTGGTTGGTGCTTAATGCCGCATCCGGGCTGCAAAACCAATGGGCTGACGACTTACCCCAAC                     <  SRR4302537.579478/94‑1 (MQ=60)
                                                                      AAAAAGCTCCCGGAGTTGGGGGCTTATGATAGTGGTTGGTGCTTAATGCCGCATCCGGGCTGCAAAACCAATGGGCTGACGACTTACCCCAACT                    >  SRR4302537.5523604/1‑94 (MQ=60)
                                                                      AAAAAGCTCCCGGAGTTGGGGGCTTATGATAGTGGTTGGTGCTTAATGCCGCATCCGGGCTGCAAAACCAATGGGCTGACGACTTACCCCAACT                    <  SRR4302537.592447/94‑1 (MQ=60)
                                                                       AAAAGCTCCCGGAGTTGGGGGCTTATGATAGTGGTTGGTGCTTAATGCCGCATCCGGGCTGCAAAACCAATGGGCTGACGACTTACCCCAACTG                   <  SRR4302537.2721938/94‑1 (MQ=60)
                                                                        AAAGCTCCCGGAGTTGGGGGCTTATGATAGTGGTTGGTGCTTAATGCCGCATCCGGGCTGCAAAACCAATGGGCTGACGACTTACCCCAACTGC                  >  SRR4302537.2999171/1‑94 (MQ=60)
                                                                        AAAGCTCCCGGAGTTGGGGGCTTATGATAGTGGTTGGTGCTTAATGCCGCATCCGGGCTGCAAAACCAATGGGCTGACGACTTACCCCAACTGC                  >  SRR4302537.4981884/1‑94 (MQ=60)
                                                                          AGCTCCCGGAGTTGGGGGCTTATGATAGTGGTTGGTGCTTAATGCCGCATCCGGGCTGCAAAACCAATGGGCTGACGACTTACCCCAACTGCTT                <  SRR4302537.6319118/94‑1 (MQ=60)
                                                                             TCCCGGAGTTGGGGGCTTATGATAGTGGTTGGTGCTTAATGCCGCATCCGGGCTGCAAAACCAATGGGCTGACGACTTACCCCAACTGCTTACG             >  SRR4302537.122397/1‑94 (MQ=60)
                                                                               CCGGAGTTGGGGGCTTATGATAGTGGTTGGTGCTTAATGCCGCATCCGGGCTGCAAAACCAATGGGCTGACGACTTACCCCAACTGCTTACTCG           >  SRR4302537.2300936/1‑94 (MQ=60)
                                                                               CCGGAGTTGGGGGCTTATGATAGTGGTTGGTGCTTAATGCCGCATCCGGGCTGCAAAACCAATGGGCTGACGACTTACCCCAACTGCTTACGCG           <  SRR4302537.4241939/94‑1 (MQ=60)
                                                                                CGGAGTTGGGGGCTTATGATAGTGGTTGGTGCTTAATGCCGCATCCGGGCTGCAAAACCAATGGGCTGACGACTTACCCCAACTGCTTACGCGC          <  SRR4302537.4686173/94‑1 (MQ=60)
                                                                                 GGAGTTGGGGGCTTATGATAGTGGTTGGTGCTTAATGCCGCATCCGGGCTGCAAAACCAATGGGCTGACGACTTACCCCAACTGCTTACGCGCA         >  SRR4302537.1534641/1‑94 (MQ=60)
                                                                                 GGAGTTGGGGGCTTATGATAGTGGTTGGTGCTTAATGCCGCATCCGGGCTGCAAAACCAATGGGCTGACGACTTACCCCAACTGCTTACGCGCA         >  SRR4302537.6188680/1‑94 (MQ=60)
                                                                                  GAGTTGGGGGCTTATGATAGTGGTTGGTGCTTAATGCCGCATCCGGGCTGCAAAACCAATGGGCTGACGACTTACCCCAACTGCTTACGCGCAT        >  SRR4302537.4687425/1‑94 (MQ=60)
                                                                                  GAGTTGGGGGCTTATGATAGTGGTTGGTGCTTAATGCCGCATCCGGGCTGCAAAACCAATGGGCTGACGACTTACCCCAACTGCTTACGCGCAT        <  SRR4302537.1213425/94‑1 (MQ=60)
                                                                                   AGTTGGGGGCTTATGATAGTGGTTGGTGCTTAATGCCGCATCCGGGCTGCAAAACCAATGGGCTGACGACTTACCCCAACTGCTTACGCGCATT       <  SRR4302537.2573704/94‑1 (MQ=60)
                                                                                   AGTTGGGGGCTTATGATAGTGGTTGGTGCTTAATGCCGCATCCGGGCTGCAAAACCAATGGGCTGACGACTTACCCCAACTGCTTACGCGCATT       <  SRR4302537.6440690/94‑1 (MQ=60)
                                                                                   AGTTGGGAGCTTATGATAGTGGTTGGTGCTTAATGCCGCATCCGGGCTGCAAAACCAATGGGCTGACGACTTACCCCAACTGCTTACGCGCATT       >  SRR4302537.927183/1‑94 (MQ=60)
                                                                                     TTGGGGGCTTATGATAGTGGTTGGTGCTTAATGCCGCATCCGGGCTGCAAAACCAATGGGCTGACGACTTACCCCAACTGCTTACGCGCATTGC     >  SRR4302537.4166003/1‑94 (MQ=60)
                                                                                     GTGGGGGCTTATGATAGTGGTTGGTGCTTAATGCCGCATCCGGGCTGCAAAACCAATGGGCTGACGACTTACCCCAACTGCTTACGCGCATTGC     <  SRR4302537.2445152/94‑1 (MQ=60)
                                                                                       GGGGGCTTATGATAGTGGTTGGTGCTTAATGCCGCATCCGGGCTGCAAAACCAATGGGCTGACGACTTACCCCAACTGCTTACGCGCATTGCGG   <  SRR4302537.4854460/94‑1 (MQ=60)
                                                                                       GGGGGCTTATGATAGTGGTTGGTGCTTAATGCCGCATCCGGGCTGCAAAACCAATGGGCTGACGACTTACCCCAACTGCTTACGCGCATTGCGG   <  SRR4302537.1278076/94‑1 (MQ=60)
                                                                                        GGGGCTTATGATAGTGGTTGGTGCTTAATGCCGCATCCGGGCTACAAAACCAATGGGCTGACGACTTACCCCAACTGCTTACGCGCATTGCGGA  >  SRR4302537.4574811/1‑94 (MQ=60)
                                                                                          |                                                                                           
AAAATCAATGAGTTAAGTGTCGCCAGAAAGCGACACGGCAAACCACCCATTGTCGTGATTTACAGACACAAAAAAGCTCCCGGAGTTGGGAGCTTATGATAGTGGTTGGTGCTTAATGCCGCATCCGGGCTGCAAAACCAATGGGCTGACGACTTACCCCAACTGCTTACGCGCATTGCGGA  >  NC_000913/2689523‑2689704

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑