Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I2 R1 882 254.4 12957588 98.0% 12698436 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,694,185 G→C G76A (GGC→GCC)  yfhD → putative periplasmic binding transport protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,694,1850GC96.0% 670.3 / 15.7 202G76A (GGC→GCC) yfhDputative periplasmic binding transport protein
Reads supporting (aligned to +/- strand):  ref base G (6/2);  new base C (106/88);  total (112/90)
Fisher's exact test for biased strand distribution p-value = 3.03e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.37e-02

Only 100 of 215 total aligned reads displayed.

CAGCTGTTTGACGACCTTGATAATGGTAACGCCGACCTGCTGGCGGCAGGACTTGTCTATAACAGTGAGCGGGTAAAAAATTATCAGCCTGGCCCTACCTATTATTCCGTGTCACAACAACTGGTTTATAAAGTGGGTCAGTATCGCCCACGTACGCTGGGCAACCTGACGGCGGAGCAAC  >  NC_000913/2694094‑2694274
                                                                                           |                                                                                         
cagcTGTTTGACGACCTTGATAATGGTAACGCCGACCTGCTGGCGGCAGGACTTGTCTATAACAGTGAGCGGGTAAAAAATTATCAGCCTGGc                                                                                          <  1:5776181/93‑1 (MQ=255)
cagcTGTTTGACGACCTTGATAATGGTAACGCCGACCTGCTGGCGGCAGGACTTGTCTATAACAGTGAGCGGGGAAAAAATTATCAGCCTGGc                                                                                          >  2:4154120/1‑93 (MQ=255)
 agcTGTTTGACGACCTTGATAATGGTAACGCCGACCTGCTGGCGGCAGGACTTGTCTATAACAGTGAGCGGGTAAAAAATTATCAGCCTGccc                                                                                         >  2:5353628/1‑93 (MQ=255)
  gcTGTTTGACGACCTTGATAATGGTAACGCCGACCTGCTGGCGGCAGGACTTGTCTATAACAGTGAGCGGGTAAAAAATTATCAGCCTGcccc                                                                                        >  2:5213656/1‑93 (MQ=255)
    tGTTTGACGACCTTGATAATGGTAACGCCGACCTGCTGGCGGCAGGACTTGTCTATAACAGTGAGCGGGTAAAAAATTATCAGCCTGCCccta                                                                                      >  1:1703822/1‑93 (MQ=255)
    tGTTTGACGACCTTGATAATGGTAACGCCGACCTGCTGGCGGCAGGACTTGTCTATAACAGTGAGCGGGTAAAAAATTATCAGCCTGCCccta                                                                                      >  2:2250347/1‑93 (MQ=255)
    tGTTTGACGACCTTGATAATGGTAACGCCGACCTGCTGGCGGCAGGACTTGTCTATAACAGTGAGCGGGTAAAAAATTATCAGCCTGCCccta                                                                                      <  2:1532114/93‑1 (MQ=255)
     gTTTGACGACCTTGATAATGGTAACGCCGACCTGCTGGCGGCAGGACTTGTCTATAACAGTGAGCGGGTAAAAAATTATCAGCCTGCCcctac                                                                                     >  1:1697622/1‑93 (MQ=255)
      tttGACGACCTTGATAATGGTAACGCCGACCTGCTGGCGGCAGGACTTGTCTATAACAGTGAGCGGGTAAAAAATTATCAGCCTGCCcctacc                                                                                    <  1:2366690/93‑1 (MQ=255)
        tGACGACCTTGATAATGGTAACGCCGACCTGCTGGCGGCAGGACTTGTCTATAACAGTGAGCGGTTAAAAAATTATCAGCCTGCCcctaccta                                                                                  <  2:1104182/93‑1 (MQ=255)
        tGACGACCTTGATAATGGTAACGCCGACCTGCTGGCGGCAGGACTTGTCTATAACAGTGAGCGGGTAAAAAATTATCAGCCTGCCcctaccta                                                                                  <  1:999052/93‑1 (MQ=255)
         gacgacCTTGATAATGGTAACGCCGACCTGCTGGCGGCAGGACTTGTCTATAACAGTGAGCGGGTAAAAAATTATCAGCCTGCCCCTACCtat                                                                                 <  2:3579369/93‑1 (MQ=255)
           cgacCTTGATAATGGTAACGCCGACCTGCTGGCGGCAGGACTTGTCTATAACAGTGAGCGGGTAAAAAATTATCAGCCTGCCCCTACCtatta                                                                               >  2:1247345/1‑93 (MQ=255)
           cgacCTTGATAATGGTAACGCCGACCTGCTGGCGGCAGGACTTGTCTATAACAGTGAGCGGGTAAAAAATTATCAGCCTGCCCCTACCtatta                                                                               <  2:2623198/93‑1 (MQ=255)
            gacCTTGATAATGGTAACGCCGACCTGCTGGCGGCAGGACTTGTCTATAACAGTGAGCGGGTAAAAAATTATCAGCCTGCCCCTACCtattat                                                                              >  2:1310895/1‑93 (MQ=255)
            gacCTTGATAATGGTAACGCCGACCTGCTGGCGGCAGGACTTGTCTATAACAGTGAGCGGGTAAAAAATTATCAGCCTGCCCCTACCtattat                                                                              >  1:5547941/1‑93 (MQ=255)
             acCTTGATAATGGTAACGCCGACCTGCTGGCGGCAGGACTTGTCTATAACAGTGAGCGGGTAAAAAATTATCAGCCTGCCCCTACCTattatt                                                                             >  1:81230/1‑93 (MQ=255)
             acCTTGATAATGGTAACGCCGACCTGCTGGCGGCAGGACTTGTCTATAACAGTGAGCGGGTAAAAAATTATCAGCCTGCCCCTACCTattatt                                                                             >  2:4133951/1‑93 (MQ=255)
             acCTTGATAATGGTAACGCCGACCTGCTGGCGGAAGGACTTGTCTATAACAGTGAGCGGGTAAAAAATTATCAGCCTGCCCCTACCTattatt                                                                             >  1:6161425/1‑93 (MQ=255)
                ttGATAATGGTAACGCCGACCTGCTGGCGGCAGGACTTGTCTATAACAGTGAGCGGGTAAAAAATTATCAGCCTGCCCCTACCTATTATTCCg                                                                          >  2:1870831/1‑93 (MQ=255)
                 tGATAATGGTAACGCCGACCTGCTGGCGGCAGGACTTGTCTATAACAGTGAGCGGGTAAAAAATTATCAGCCTGCCCCTACCTATTATTCCgt                                                                         >  1:5864277/1‑93 (MQ=255)
                  gATAATGGTAACGCCGACCTGCTGGCGGCAGGACTTGTCTATAACAGTGAGCGGGTAAAAAATTATCAGCCTGCCCCTACCTATTATTCCgtg                                                                        >  1:3673950/1‑93 (MQ=255)
                    tAATGGTAACGCCGACCTGCTGGCGGCAGGACTTGTCTATAACAGTGAGCGGGTAAAAAATTATCAGCCTGCCCCTACCTATTATTCCGTGTc                                                                      >  2:1836282/1‑93 (MQ=255)
                    tAATGGTAACGCCGACCTGCTGGCGGCAGGACTTGTCTATAACAGTGAGCGGGTAAAAAATTATCAGCCTGCCCCTACCTATTATTCCGTGTc                                                                      >  2:479441/1‑93 (MQ=255)
                    tAATGGTAACGCCGACCTGCTGGCGGCAGGACTTGTCTATAACAGTGAGCGGGTAAAAAATTATCAGCCTGCCCCTACCTATTATTCCGTGTc                                                                      >  2:369865/1‑93 (MQ=255)
                     aaTGGTAACGCCGACCTGCTGGCGGCAGGACTTGTCTATAACAGTGAGCGGGTAAAAAATTATCAGCCTGCCCCTACCTATTATTCCGTGTca                                                                     >  2:3274031/1‑93 (MQ=255)
                      aTGGTAACGCCGACCTGCTGGCGGCAGGACTTGTCTATAACAGTGAGCGGGTAAAAAATTATCAGCCTGCCCCTACCTATTATTCCGTGTcac                                                                    >  2:1131729/1‑93 (MQ=255)
                            aCGCCGACCTGCTGGCGGCAGGACTTGTCTATAACAGTGAGCGGGTAAAAAATTATCAGCCTGCCCCTACCTATTATTCCGTGTCACaacaac                                                              <  1:1023070/93‑1 (MQ=255)
                            aCGCCGACCTGCTGGCGGCAGGACTTGTCTATAACAGTGAGCGGGTAAAAAATTATCAGCCTGCCCCTACCTATTATTCCGTGTCACaacaac                                                              >  2:1778286/1‑93 (MQ=255)
                            aCGCCGACCTGCTGGCGGCAGGACTTGTCTATAACAGTGAGCGGGTAAAAAATTATCAGCCTGCCCCTACCTATTATTCCGTGTCACaacaac                                                              <  2:5885082/93‑1 (MQ=255)
                             cGCCGACCTGCTGGCGGCAGGACTTGTCTATAACAGTGAGCGGGTAAAAAATTATCAGCCTGCCCCTACCTATTATTCCGTGTCACAACAACt                                                             >  2:447721/1‑93 (MQ=255)
                              gCCGACCTGCTGGCGGCAGGACTTGTCTATAACAGTGAGCGGGTAAAAAATTATCAGCCTGCCCCTACCTATTATTCCGTGTCACAACAACTg                                                            >  1:2429522/1‑93 (MQ=255)
                              gCCGACCTGCTGGCGGCAGGACTTGTCTATAACAGTGAGCGGGTAAAAAATTATCAGCCTGCCCCTACCTATTATTCCGTGTCACAACAACTg                                                            >  1:2627361/1‑93 (MQ=255)
                              gCCGACCTGCTGGCGGCAGGACTTGTCTATAACAGTGAGCGGGTAAAAAATTATCAGCCTGCCCCTACCTATTATTCCGTGTCACAACAACTg                                                            <  1:6113727/93‑1 (MQ=255)
                               ccGACCTGCTGGCGGCAGGACTTGTCTATAACAGTGAGCGGGTAAAAAATTATCAGCCTGCCCCTACCTATTATTCCGTGTCACAACAACTgg                                                           >  1:4290067/1‑93 (MQ=255)
                                cGACCTGCTGGCGGCAGGACTTGTCTATAACAGTGAGCGGGTAAAAAATTATCAGCCTGCCCCTACCTATTATTCCGTGTCACAACAACTGGt                                                          <  2:407607/93‑1 (MQ=255)
                                cGACCTGCTGGCGGCAGGACTTGTCTATAACAGTGAGCGGGTAAAAAATTATCAGCCTGCCCCTACCTATTATTCCGTGTCACAACAACTGGt                                                          <  2:5060571/93‑1 (MQ=255)
                                 gACCTGCTGGCGGCAGGACTTGTCTATAACAGTGAGCGGGTAAAAAATTATCAGCCTGCCCCTACCTATTATTCCGTGTCACAACAACTGGtt                                                         <  1:3848872/93‑1 (MQ=255)
                                  aCCTGCTGGCGGCAGGACTTGTCTATAACAGTGAGCGGGTAAAAAATTATCAGCCTGCCCCTACCTATTATTCCGTGTCACAACAACTGGttt                                                        >  2:4846280/1‑93 (MQ=255)
                                    ctgctgGCGGCAGGACTTGTCTATAACAGTGAGCGGGTAAAAAATTATCAGCCTGCCCCTACCTATTATTCCGTGTCACAACAACTGGTTtat                                                      <  1:1749503/93‑1 (MQ=255)
                                    ctgctgGCGGCAGGACTTGTCTATAACAGTGAGCGGGTAAAAAATTATCAGCCTGCCCCTACCTATTATTCCGTGTCACAACAACTGGTTtat                                                      <  1:4991514/93‑1 (MQ=255)
                                     tgctgGCGGCAGGACTTGTCTATAACAGTGAGCGGGTAAAAAATTATCAGCCTGCCCCTACCTATTATTCCGTGTCACAACAACTGGTTtata                                                     <  2:4180593/93‑1 (MQ=255)
                                     tgctgGCGGCAGGACTTGTCTATAACAGTGAGCGGGTAAAAAATTATCAGCCTGCCCCTACCTATTATTCCGTGTCACAACAACTGGTTtata                                                     >  2:5877264/1‑93 (MQ=255)
                                     tgctgGCGGCAGGACTTGTCTATAACAGTGAGCGGGTAAAAAATTATCAGCCTGCCCCTACCTATTATTCCGTGTCACAACAACTGGTTtata                                                     >  2:5406799/1‑93 (MQ=255)
                                     tgctgGCGGCAGGACTTGTCTATAACAGTGAGCGGGTAAAAAATTATCAGCCTGCCCCTACCTATTATTCCGTGTCACAACAACTGGTTtata                                                     <  2:2966212/93‑1 (MQ=255)
                                     tgctgGCGGCAGGACTTGTCTATAACAGTGAGCGGGTAAAAAATTATCAGCCTGCCCCTACCTATTATTCCGTGTCACAACAACTGGTTtata                                                     <  1:3465482/93‑1 (MQ=255)
                                      gctgGCGGCAGGACTTGTCTATAACAGTGAGCGGGTAAAAAATTATCAGCCTGCCCCTACCTATTATTCCGTGTCACAACAACTGGTTTATaa                                                    <  2:5371022/93‑1 (MQ=255)
                                      gctgGCGGCAGGACTTGTCTATAACAGTGAGCGGGTAAAAAATTATCAGCCTGCCCCTACCTATTATTCCGTGTCACAACAACTGGTTTATaa                                                    >  2:3010187/1‑93 (MQ=255)
                                        tgGCGGCAGGACTTGTCTATAACAGTGAGCGGGTAAAAAATTATCAGCCTGCCCCTACCTATTATTCCGTGTCACAACAACTGGTTTATAAAg                                                  >  1:1462165/1‑93 (MQ=255)
                                          gcggcAGGACTTGTCTATAACAGTGAGCGGGTAAAAAATTATCAGCCTGCCCCTACCTATTATTCCGTGTCACAACAACTGGTTTATAAAGTg                                                <  2:2661662/93‑1 (MQ=255)
                                           cggcAGGACTTGTCTATAACAGTGAGCGGGTAAAAAATTATCAGCCTGCCCCTACCTATTATTCCGTGTCACAACAACTGGTTTATAAAGTgg                                               <  1:2240586/93‑1 (MQ=255)
                                           cggcAGGACTTGTCTATAACAGTGAGCGGGTAAAAAATTATCAGCCTGCCCCTACCTATTATTCCGTGTCACAACAACTGGTTTATAAAGTgg                                               <  1:1257669/93‑1 (MQ=255)
                                            ggcAGGACTTGTCTATAACAGTGAGCGGGTAAAAAATTATCAGCCTGCCCCTACCTATTATTCCGTGTCACAACAACTGGTTTATAAAGTggg                                              <  1:185041/93‑1 (MQ=255)
                                             gcAGGACTTGTCTATAACAGTGAGCGGGTAAAAAATTATCAGCCTGCCCCTACCTATTATTCCGTGTCACAACAACTGGTTTATAAAGTGGGt                                             <  2:4444154/93‑1 (MQ=255)
                                              cAGGACTTGTCTATAACAGTGAGCGGGTAAAAAATTATCAGCCTGCCCCTACCTATTATTCCGTGTCACAACAACTGGTTTATAAAGTGGGTc                                            <  1:2998805/93‑1 (MQ=255)
                                                ggACTTGTCTATAACAGTGAGCGGGTAAAAAATTATCAGCCTGCCCCTACCTATTATTCCGTGTCACAACAACTGGTTTATAAAGTGGGTCAg                                          <  2:5272636/93‑1 (MQ=255)
                                                ggACTTGTCTATAACAGTGAGCGGGTAAAAAATTATCAGCCTGCCCCTACCTATTATTCCGTGTCACAACAACTAGTTTATAAAGTGGATCAg                                          >  1:4120953/1‑93 (MQ=255)
                                                  aCTTGTCTATAACAGTGAGCGGGTAAAAAATTATCAGCCTGCCCCTACCTATTATTCCGTGTCACAACAACTGGTTTATAAAGTGGGTCAGTa                                        <  1:737178/93‑1 (MQ=255)
                                                   cTTGTCTATAACAGTGAGCGGGTAAAAAATTATCAGCCTGCCCCTACCTATTATTCCGTGTCACAACAACTGGTTTATAAAGTGGGTCAGTAt                                       >  2:4480344/1‑93 (MQ=255)
                                                    ttGTCTATAACAGTGAGCGGGTAAAAAATTATCAGCCTGCCCCTACCTATTATTCCGTGTCACAACAACTGGTTTATAAAGTGGGTCAGTATc                                      >  2:143557/1‑93 (MQ=255)
                                                    ttGTCTATAACAGTGAGCGGGTAAAAAATTATCAGCCTGCCCCTACCTATTATTCCGTGTCACAACAACTGGTTTATAAAGTGGGTCAGTATc                                      >  1:2334481/1‑93 (MQ=255)
                                                    ttGTCTATAACAGTGAGCGGGTAAAAAATTATCAGCCTGCCCCTACCTATTATTCCGTGTCACAACAACTGGTTTATAAAGTGGGTCAGTATc                                      <  2:5736079/93‑1 (MQ=255)
                                                    gttgcTATAACAGGTAGCGGGTAAAAAATTATCAGCCTGCCCATACCTATTATGCCGTGTCACAACAACTGGTTTATAAAGTGGGTCAGTATc                                      <  1:1420125/89‑1 (MQ=255)
                                                      gTCTATAACAGTGAGCGGGTAAAAAATTATCAGCCTGCCCCTACCTATTATTCCGTGTCACAACAACTGGTTTATAAAGTGGGTCAGTATCGc                                    <  2:3520342/93‑1 (MQ=255)
                                                      gTCTATAACAGTGAGCGGGTAAAAAATTATCAGCCTGCCCCTACCTATTATTCCGTGTCACAACAACTGGTTTATAAAGTGGGTCAGTATCGc                                    <  1:6238588/93‑1 (MQ=255)
                                                        cTATAACAGTGAGCGGGTAAAAAATTATCAGCCTGCCCCTACCTATTATTCCGTGTCACAACAACTGGTTTATAAAGTGGGTCAGTATCGccc                                  >  1:4908067/1‑93 (MQ=255)
                                                         tataACAGTGAGCGGGTAAAAAATTATCAGACTGCCCCTACCTATTATTCCGTGTCACAACAACTGGTTTATAAAGTGTGTCAGTATCGCCCa                                 >  2:3440258/1‑93 (MQ=255)
                                                           taACAGTGAGCGGGTAAAAAATTATCAGCCTGCCCCTACCTATTATTCCGTGTCACAACAACTGGTTTATAAAGTGGGTCAGTATCGCCCACg                               >  1:5632046/1‑93 (MQ=255)
                                                           taACAGTGAGCGGGTAAAAAATTATCAGCCTGCCCCTACCTATTATTCCGTGTCACAACAACTGGTTTATAAAGTGGGTCAGTATCGCCCACg                               <  1:4658551/93‑1 (MQ=255)
                                                             aCAGTGAGCGGGTAAAAAATTATCAGCCTGCCCCTACCTATTATTCCGTGTCACAACAACTGGTTTATAAAGTGGGTCAGTATCGCCCACGTa                             >  2:2808859/1‑93 (MQ=255)
                                                             aCAGTGAGCGGGTAAAAAATTATCAGCCTGCCCCTACCTATTATTCCGTGTCACAACAACTGGTTTATAAAGTGGGTCAGTATCGCCCACGTa                             <  1:3610841/93‑1 (MQ=255)
                                                               aGTGAGCGGGTAAAAAATTATCAGCCTGCCCCTACCTATTATTCCGTGTCACAACAACTGGTTTATAAAGTGGGTCAGTATCGCCCACGTACg                           >  1:1388/1‑93 (MQ=255)
                                                                gTGAGCGGGTAAAAAATTATCAGCCTGCCCCTACCTATTATTCCGTGTCACAACAACTGGTTTATAAAGTGGGTCAGTATCGCCCACGTACGc                          >  1:6324232/1‑93 (MQ=255)
                                                                gTGAGCGGGTAAAAAATTATCAGCCTGCCCCTACCTATTATTCCGTGTCACAACAACTGGTTTATAAAGTGGGTCAGTATCGCCCACGTACGc                          >  1:4800793/1‑93 (MQ=255)
                                                                  gAGCGGGTAAAAAATTATCAGCCTGCCCCTACCTATTATTCCGTGTCACAACAACTGGTTTATAAAGTGGGTCAGTATCGCCCACGTACGCTg                        <  2:3254309/93‑1 (MQ=255)
                                                                  gAGCGGGTAAAAAATTATCAGCCTGCCCCTACCTATTATTCCGTGTCACAACAACTGGTTTATAAAGTGGGTCAGTATCGCCCACGTACGCTg                        <  2:563163/93‑1 (MQ=255)
                                                                  gAGCGGGTAAAAAATTATCAGCCTGCCCCTACCTATTATTCCGTGTCACAACAACTGGTTTATAAAGTGGGTCAGTATCGCCCACGTACGCTg                        <  1:548073/93‑1 (MQ=255)
                                                                   aGCGGGTAAAAAATTATCAGCCTGCCCCTACCTATTATTCCGTGTCACAACAACTGGTTTATAAAGTGGGTCAGTATCGCCCACGTACGCTgg                       <  1:1341366/93‑1 (MQ=255)
                                                                    gCGGGTAAAAAATTATCAGCCTGCCCCTACCTATTATTCCGTGTCACAACAACTGGTTTATAAAGTGGGTCAGTATCGCCCACGTACGCTggg                      >  1:5193427/1‑93 (MQ=255)
                                                                     ggggTAAAAAATTATCAGCCTGCCCCTACCTATTATTCCGTGTCACAACAACTGGTTTATAAAGTGGGTCAGTATCGCCCACGTACGCTGGGc                     <  1:1149789/92‑1 (MQ=255)
                                                                      gggTAAAAAATTATCAGCCTGCCCCTACCTATTATTCCGTGTCACAACAACTGGTTTATAAAGTGGGTCAGTATCGCCCACGTACGCTGGGCa                    >  2:4323850/1‑93 (MQ=255)
                                                                         tAAAAAATTATCAGCCTGCCCCTACCTATTATTCCGTGTCACAACAACTGGTTTATAAAGTGGGTCAGTATCGCCCACGTACGCTGGGCAAcc                 <  1:2068219/93‑1 (MQ=255)
                                                                          aaaaaaTTATCAGCCTGCCCCTACCTATTATTCCGTGTCACAACAACTGGTTTATAAAGTGGGTCAGTATCGCCCACGTACGCTGGGCAACCt                >  2:3809552/1‑93 (MQ=255)
                                                                          aaaaaaTTATCAGCCTGCCCCTACCTATTATTCCGTGTCACAACAACTGGTTTATAAAGTGGGTCAGTATCGCCCACGTACGCTGGGCAACCt                <  2:1194804/93‑1 (MQ=255)
                                                                          aaaaaaTTATCAGCCTCCCCCTACCTATTATCCCGTGTCACAACAACTGGTTTATAAAGTGGGTCAGTATCGCCCACGTACGCTGGGCAACCt                <  2:2535390/93‑1 (MQ=255)
                                                                           aaaaaTTATCAGCCTGCCCCTACCTATTATTCCGTGTCACAACAACTGGTTTATAAAGTGGGTCAGTATCGCCCACGTACGCTGGGCAACCTg               >  2:4510661/1‑93 (MQ=255)
                                                                           aaaaaTTATCAGCCTGCCCCTACCTATTATTCCGTGTCACAACAACTGGTTTATAAAGTGGGTCAGTATCGCCCACGTACGCTGGGCAACCTg               <  1:4442714/93‑1 (MQ=255)
                                                                             aaaTTATCAGCCTGCCCCTACCTATTATTCCGTGTCACAACAACTGGTTTATAAAGTGGGTCAGTATCGCCCACGTACGCTGGGCAACCTGAc             <  1:5081422/93‑1 (MQ=255)
                                                                              aaTTATCAGCCTGCCCCTACCTATTATTCCGTGTCACAACAACTGGTTTATAAAGTGGGTCAGTATCGCCCACGTACGCTGGGCAACCTGAcg            <  2:6169723/93‑1 (MQ=255)
                                                                              aaTTATCAGCCTGCCCCTACCTATTATTCCGTGTCACAACAACTGGTTTATAAAGTGGGTCAGTATCGCCCACGTACGCTGGGCAACCTGAcg            >  2:4999686/1‑93 (MQ=255)
                                                                              aaTTATCAGCCTGCCCCTACCTATTATTCCGTGTCACAACAACTGGTTTATAAAGTGGGTCAGTATCGCCCACGTACGCTGGGCAACCTGAcg            >  1:953533/1‑93 (MQ=255)
                                                                              aaTTATCAGCCTGCCCCTACCTATTATTCCGTGTCACAACAACTGGTTTATAAAGTGGGTCAGTATCGCCCACGTACGCTGGGCAACCTGAcg            <  1:3637822/93‑1 (MQ=255)
                                                                              aaTTATCAGCCTGCCCCTACCTATTATTCCGTGTCACAACAACTGGTTT‑TTAAGTGGGGCAGTATCCGCCACGTACGCTTGGCAACCTGAcgg           >  2:6375931/1‑93 (MQ=255)
                                                                                  aTCAGCCTGCCCCTACCTATTATTCCGTGTCACAACAACTGGTTTATAAAGTGGGTCAGTATCGCCCACGTACGCTGGGCAACCTGAcggcgg        <  1:1910502/93‑1 (MQ=255)
                                                                                    cAGCCTGGCCCTACCTATTATTCCGTGTCACAACAACTGGTTTATAAAGTGGGTCAGTATCGCCCACGTACGCTGGGCAACCTGACGGCGGAg      >  1:5064711/1‑93 (MQ=255)
                                                                                     aGCCTGCCCCTACCTATTATTCCGTGTCACAACAACTGGTTTATAAAGTGGGTCAGTATCGCCCACGTACGCTGGGCAACCTGACGGCGGAGc     <  1:5568265/93‑1 (MQ=255)
                                                                                     aGCCTGCCCCTACCTATTATTCCGTGTCACAACAACTGGTTTATAAAGTGGGTCAGTATCGCCCACGTACGCTGGGCAACCTGACGGCGGAGc     >  1:2055281/1‑93 (MQ=255)
                                                                                      gCCTGCCCCTACCTATTATTCCGTGTCACAACAACTGGTTTATAAAGTGGGTCAGTATCGCCCACGTACGCTGGGCAACCTGACGGCGGAgga    >  2:2455736/1‑91 (MQ=255)
                                                                                      gCCTGCCCCTACCTATTATTCCGTGTCACAACAACTGGTTTATAAAGTGGGTCAGTATCGCCCACGTACGCTGGGCAACCTGACGGCGGAGCa    >  2:4393281/1‑93 (MQ=255)
                                                                                       ccTGCCCCTACCTATTATTCCGTGTCACAACGACTGGTTTATAAAGTGGGTCAGTATCGCCCACGTACGCTGGGCAACCTGACGGCGGAGCaa   <  2:3360854/93‑1 (MQ=255)
                                                                                           |                                                                                         
CAGCTGTTTGACGACCTTGATAATGGTAACGCCGACCTGCTGGCGGCAGGACTTGTCTATAACAGTGAGCGGGTAAAAAATTATCAGCCTGGCCCTACCTATTATTCCGTGTCACAACAACTGGTTTATAAAGTGGGTCAGTATCGCCCACGTACGCTGGGCAACCTGACGGCGGAGCAAC  >  NC_000913/2694094‑2694274

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
GCCAGCTGTTTGACGACCTTGATAATGGTAACGCCGACCTGCTGGCGGCAGGACTTGTCTATAACAGTGAGCGGGTAAAAAATTATCAGCCTGGCCCTACCTATTATTCCGTGTCACAACAACTGGTTTATAAAGTGGGTCAGTATCGCCCACGTACGCTGGGCAACCTGACGGCGGAGCAACTCAC  >  NC_000913/2694092‑2694278
                                                                                             |                                                                                             
GCCAGCTGTTTGACGACCTTGATAATGGTAACGCCGACCTGCTGGCGGCAGGACTTGTCTATAACAGTGAGCGGGTAAAAAATTATCAGCCTGC                                                                                               >  SRR4302537.2487997/1‑94 (MQ=60)
GCCAGCTGTTTGACGACCTTGATAATGGTAACGCCGACCTGCTGGCGGCAGGACTTGTCTATAACAGTGAGCGGGTAAAAAATTATCAGCCTGC                                                                                               >  SRR4302537.339348/1‑94 (MQ=60)
 CCAGCTGTTTGACGACCTTGATAATGGTAACGCCGACCTGCTGGCGGCAGGACTTGTCTATAACAGTGAGCGGGTAAAAAATTATCAGCCTGCC                                                                                              >  SRR4302537.2894932/1‑94 (MQ=60)
  CAGCTGTTTGACGACCTTGATAATGGTAACGCCGACCTGCTGGCGGCAGGACTTGTCTATAACAGTGAGCGGGTAAAAAATTATCAGCCTGGCC                                                                                             <  SRR4302537.5815775/94‑1 (MQ=60)
  CAGCTGTTTGACGACCTTGATAATCGTAACGCCGACCTGCTGGCGGCAGGACTTGTCTATAACAGTGAGCGGGTAAAAAATTATCAGCCTGCCC                                                                                             >  SRR4302537.1037841/1‑94 (MQ=60)
    GCTGTTTGACGACCTTGATAATGGTAACGCCGACCTGCTGGCGGCAGGACTTGTCTATAACAGTGAGCGGGTAAAAAATTATCAGCCTGCCCCT                                                                                           >  SRR4302537.2143029/1‑94 (MQ=60)
    GCTGTTTGACGACCTTGATAATGGTAACGCCGACCTGCTGGCGGCAGGACTTGTCTATAACAGTGAGCGGGTAAAAAATTATCAGCCTGCCCCT                                                                                           <  SRR4302537.5002597/94‑1 (MQ=60)
     CTGTTTGACGACCTTGATAATGGTAACGCCGACCTGCTGGCGGCAGGACTTGTCTATAACAGTGAGCGGGTAAAAAATTATCAGCCTGCCCCTA                                                                                          >  SRR4302537.3676022/1‑94 (MQ=60)
     CTGTTTGACGACCTTGATAATGGTAACGCCGACCTGCTGGCGGCAGGACTTGTCTATAACAGTGAGCGGGTAAAAAATTATCAGCCTGCCCCTA                                                                                          >  SRR4302537.1715731/1‑94 (MQ=60)
      TGTTTGACGACCTTGATAATGGTAACGCCGACCTGCTGGCGGCAGGACTTGTCTATAACAGTGAGCGGGTAAAAAATTATCAGCCTGCCCCTAC                                                                                         >  SRR4302537.1709495/1‑94 (MQ=60)
       GTTTGACGACCTTGATAATGGTAACGCCGACCTGCTGGCGGCAGGACTTGTCTATAACAGTGAGCGGGTAAAAAATTATCAGCCTGGCCCTACC                                                                                        <  SRR4302537.4320406/94‑1 (MQ=60)
        TTTGACGACCTTGATAATGGTAACGCCGACCTGCTGGCGGCAGGACTTGTCTATAACAGTGAGCGGGTAAAAAATTATCAGCCTGCCCCTACCT                                                                                       <  SRR4302537.2383373/94‑1 (MQ=60)
         TTGACGACCTTGATAATGGTAACGCCGACCTGCTGGCGGCAGGACTTGTCTATAACAGTGAGCGGGTAAAAAATTATCAGCCTGCCCCTACCTA                                                                                      >  SRR4302537.5555691/1‑94 (MQ=60)
          TGACGACCTTGATAATGGTAACGCCGACCTGCTGGCGGCAGGACTTGTCTATAACAGTGAGCGGGTAAACAATTATCAGCCTGCCCCTACCTAT                                                                                     >  SRR4302537.2410831/1‑94 (MQ=60)
          TGACGACCTTGATAATGGTAACGCCGACCTGCTGGCGGCAGGACTTGTCTATAACAGTGAGCGGGTAAAAAATTATCAGCCTGCCCCTACCTAT                                                                                     <  SRR4302537.1005948/94‑1 (MQ=60)
             CGACCTTGATAATGGTAACGCCGACCTGCTGGCGGCAGGACTTGTCTATAACAGTGAGCGGGTAAAAAATTATCAGCCTGCCCCTACCTATTAT                                                                                  >  SRR4302537.153241/1‑94 (MQ=60)
             CGACCTTGATAATGGTAACGCCGACCTGCTGGCGGCAGGACTTGTCTATAACAGTGAGCGGGTAAAAAATTATCAGCCTGCCCCTACCTATTAT                                                                                  >  SRR4302537.5585822/1‑94 (MQ=60)
              GACCTTGATAATGGTAACGCCGACCTGCTGGCGGCAGGACTTGTCTATAACAGTGAGCGGGTAAAAAATTATCAGCCTGCCCCTACCTATTATT                                                                                 <  SRR4302537.5123976/94‑1 (MQ=60)
              GACCTTGATAATGGTAACGCCGACCTGCTGGCGGCAGGACTTGTCTATAACAGTGAGCGGGTAAAAAATTATCAGCCTGCCCCTACCTATTATT                                                                                 >  SRR4302537.81830/1‑94 (MQ=60)
              GACCTTGATAATGGTAACGCCGACCTGCTGGCGGCAGGACTTGTCTATAACAGTGAGCGGGTAAAAAATTATCAGCCTGCCCCTACCTATTATT                                                                                 >  SRR4302537.386892/1‑94 (MQ=60)
              GACCTTGATAATGGTAACGCCGACCTGCTGGCGGAAGGACTTGTCTATAACAGTGAGCGGGTAAAAAATTATCAGCCTGCCCCTACCTATTATT                                                                                 >  SRR4302537.6203827/1‑94 (MQ=60)
                CCTTGATAATGGTAACGCCGACCTGCTGGCGGCAGGACTTGTCTATAACAGTGAGCGGGTAAAAAATTATCAGCCTGCCCCTACCTATTATTCC                                                                               <  SRR4302537.5747682/94‑1 (MQ=60)
                 CTTGATAATGGTAACGCCGACCTGCTGGCGGCAGGACTTGTCTATAACAGTGAGCGGGTAAAAAATTATCAGCCTGCCCCTACCTATTATTCCG                                                                              <  SRR4302537.4707975/94‑1 (MQ=60)
                  TTGATAATGGTAACGCCGACCTGCTGGCGGCAGGACTTGTCTATAACAGTGAGCGGGTAAAAAATTATCAGCCTGCCCCTACCTATTATTCCGT                                                                             >  SRR4302537.5904500/1‑94 (MQ=60)
                   TGATAATGGTAACGCCGACCTGCTGGCGGCAGGACTTGTCTATAACAGTGAGCGGGTAAAAAATTATCAGCCTGCCCCTACCTATTATTCCGTG                                                                            >  SRR4302537.3699797/1‑94 (MQ=60)
                    GATAATGGTAACGCCGACCTGCTGGCGGCAGGACTTGTCTATAACAGTGAGCGGGTAAAAAATTATCAGCCTGCCCCTACCTATTATTCCGTGT                                                                           <  SRR4302537.3651075/94‑1 (MQ=60)
                           GTAACGCCGACCTGCTGGCGGCAGGACTTGTCTATAACAGTGAGCGGGTAAAAAATTATCAGCCTGCCCCTACCTATTATTCCGTGTCACAACA                                                                    >  SRR4302537.5592657/1‑94 (MQ=60)
                            TAACGCCGACCTGCTGGCGGCAGGACTTGTCTATAACAGTGAGCGGGTAAAAAATTATCAGCCTGCCCCTACCTATTATTCCGTGTCACAACAA                                                                   >  SRR4302537.5029019/1‑94 (MQ=60)
                              ACGCCGACCTGCTGGCGGCAGGACTTGTCTATAACAGTGAGCGGGTAAAAAATTATCAGCCTGCCCCTACCTATTATTCCGTGTCACAACAACT                                                                 <  SRR4302537.1030112/94‑1 (MQ=60)
                               CGCCGACCTGCTGGCGGCAGGACTTGTCTATAACAGTGAGCGGGTAAAAAATTATCAGCCTGCCCCTACCTATTATTCCGTGTCACAACAACTG                                                                >  SRR4302537.2645764/1‑94 (MQ=60)
                               CGCCGACCTGCTGGCGGCAGGACTTGTCTATAACAGTGAGCGGGTAAAAAATTATCAGCCTGCCCCTACCTATTATTCCGTGTCACAACAACTG                                                                >  SRR4302537.2446641/1‑94 (MQ=60)
                                GCCGACCTGCTGGCGGCAGGACTTGTCTATAACAGTGCGCGGGTAAAAAATTATCAGCCTGCCCCTACCTATTATTCCGTGTCACAACAACTGG                                                               >  SRR4302537.3261147/1‑94 (MQ=60)
                                GCCGACCTGCTGGCGGCAGGACTTGTCTATAACAGTGAGCGGGTAAAAAATTATCAGCCTGCCCCTACCTATTATTCCGTGTCACAACAACTGG                                                               >  SRR4302537.4319885/1‑94 (MQ=60)
                                GCCGACCTGCTGGCGGCAGGACTTGTCTATAACAGTGAGCGGGTAAAAAATTATCAGCCTGCCCCTACCTATTATTCCGTGTCACAACAACTGG                                                               <  SRR4302537.5104293/94‑1 (MQ=60)
                                GCCGACCTGCTGGCGGCAGGACTTGTCTATAACAGTGAGCGGGTAAAAAATTATCAGCCTGCCCCTACCTATTATTCCGTGTCACAACAACTGG                                                               <  SRR4302537.6155741/94‑1 (MQ=60)
                                 CCGACCTGCTGGCGGCAGGACTTGTCTATAACAGTGAGCGGGTAAAAAATTATCAGCCTGCCCCTACCTATTATTCCGTGTCCCAACCACTGGT                                                              >  SRR4302537.5822486/1‑94 (MQ=60)
                                  CGACCTGCTGGCGGCAGGACTTGTCTATAACAGTTAGCGGGTAAAAAATTATCAGCCTGCCCCTACCTATTATTCCGTGTCACAACAACTGGTT                                                             >  SRR4302537.630764/1‑94 (MQ=60)
                                   GACCTGCTGGCGGCAGGACTTGTCTATAACAGTGAGCGGGTAAAAAATTATCAGCCTGCCCCTACCTATTATTCCGTGTCACAACAANTNGTTT                                                            >  SRR4302537.1589241/1‑94 (MQ=60)
                                   GACCTGCTGGCGGCAGGACTTGTCTATAACAGTGAGCGGGTAAAAAATTATCAGCCTGCCCCTACCTATTATTCCGTGTCACAACAACTGGTTT                                                            <  SRR4302537.3875823/94‑1 (MQ=60)
                                    ACCTGCTGGCGGCAGGACTTGTCTATAACAGTGAGCGGGTAAAAAATTATCAGCCTGCCCCTACCTATTATTCCGTGTCACAACAACTGGTTTA                                                           <  SRR4302537.6192937/94‑1 (MQ=60)
                                    ACCTGCTGGCGGCAGGACTTGTCTATAACAGTGAGCGGGTAAAAAATTATCAGCCTGCCCCTACCTATTATTCCGTGTCACAACAACTGGTTTA                                                           >  SRR4302537.1361059/1‑94 (MQ=60)
                                      CTGCTGGCGGCAGGACTTGTCTATAACAGTGAGCGGGTAAAAAATTATCAGCCTGCCCCTACCTATTATTCCGTGTCACAACAACTGGTTTATA                                                         <  SRR4302537.1761703/94‑1 (MQ=60)
                                      CTGCTGGCGGCAGGACTTGTCTATAACAGTGAGCGGGTAAAAAATTATCAGCCTGCCCCTACCTATTATTCCGTGTCACAACAACTGGTTTATA                                                         <  SRR4302537.5025911/94‑1 (MQ=60)
                                      CTGCTGGCGGCAGGACTTGTCTATAACAGTGAGCGGGTAAAAAATTATCAGCCTGCCCCTACCTATTATTCCGTGTCACAACAACTGGTTTATA                                                         >  SRR4302537.3582397/1‑94 (MQ=60)
                                       TGCTGGCGGCAGGACTTGTCTATAACAGTGAGCGGGTAAAAAATTATCAGCCTGCCCCTACCTATTATTCCGTGTCACAACAACTGGTTTATAA                                                        <  SRR4302537.3489987/94‑1 (MQ=60)
                                         CTGGCGGCAGGACTTGTCTATAACAGTGAGCGGGTAAAAAATTATCAGCCTGCCCCTACCTATTATTCCGTGTCACAACAACTGGTTTATAAAG                                                      >  SRR4302537.1816523/1‑94 (MQ=60)
                                         CTGGCGGCAGGACTTGTCTATAACAGTGAGCGGGTAAAAAATTATCAGCCTGCCCCTACCTATTATTCCGTGTCACAACAACTGGTTTATAAAG                                                      >  SRR4302537.1472337/1‑94 (MQ=60)
                                           GGCGGCAGGACTTGTCTATAACAGTGAGCGGGTAAAAAATTATCAGCCTGCCCCTACCTATTATTCCGTGTCACAACAACTGGTTTATAAAGTG                                                    <  SRR4302537.2320697/94‑1 (MQ=60)
                                            GCGGCAGGACTTGTCTATAACAGTGAGCGGTTAAAAAATTATCAGCCTGCCCCTACCTATTATTCCGTGTCACAACAACTGGTTTATAAAGTGG                                                   <  SRR4302537.3135360/94‑1 (MQ=60)
                                             CGGCAGGACTTGTCTATAACAGTGAGCGGGTAAAAAATTATCAGCCTGCCCCTACCTATTATTCCGTGTCACAACAACTGGTTTATAAAGTGGG                                                  <  SRR4302537.1266426/94‑1 (MQ=60)
                                             CGGCAGGACTTGTCTATAACAGTGAGCGGGTAAAAAATTATCAGCCTGCCCCTACCTATTATTCCGTGTCACAACAACTGGTTTATAAAGTGGG                                                  <  SRR4302537.2256382/94‑1 (MQ=60)
                                             CGGCAGGACTTGTCTATAACAGTGAGCGGGTAAAAAATTATCAGCCTGCCCCTACCTATTATTCCGTGTCACAACAACTGGTTTATAAAGTGGG                                                  >  SRR4302537.2368513/1‑94 (MQ=60)
                                              GGCAGGACTTGTCTATAACAGTGAGCGGGTAAAAAATTATCAGCCTGCCCCTACCTATTATTCCGTGTCACAACAACTGGTTTATAAAGTGGGT                                                 <  SRR4302537.186359/94‑1 (MQ=60)
                                               GCAGTACTTGTCTATAACAGTGAGCGGGTAAAAAATTATCAGCCTGCCCCTACCTATTATTCCGTGTCACAACAACTGGTTTATAAAGTGGGTC                                                <  SRR4302537.3107004/94‑1 (MQ=60)
                                                CAGGACTTGTCTATAACAGTGAGCGGGTAAAAAATTATCAGCCTGCCCCTACCTATTATTCCGTGTCACAACAACTGGTTTATAAAGTGGGTCA                                               <  SRR4302537.3019711/94‑1 (MQ=60)
                                                 AGGACTTGTCTATAACAGTGAGCGGGTAAAAAATTATCAGCCTGCCCCTACCTATTATTCCGTGTCACAACAACTGGTTTATAAAGTGGGTCAG                                              <  SRR4302537.713458/94‑1 (MQ=60)
                                                 AGGACTTGTCTATAACAGTGAGCGGGTAAAAAATTATCAGCCTGCCCCTACCTATTATTCCGTGTCACAACAACTAGTTTATAAAGTGGATCAG                                              >  SRR4302537.4149725/1‑94 (MQ=60)
                                                    ACTTGTCTATAACAGTGAGCGGGTAAAAAATTATCAGCCTGCCCCTACCTATTATTCCGTGTCACAACAACTGGTTTATAAAGTGGGTCAGTAT                                           <  SRR4302537.742204/94‑1 (MQ=60)
                                                    ACTTGTCTATAACAGTGAGCGGGTAAAAAATTATCAGCCTGCCCCTACCTATTATTCCGTGTCACAACAACTGGTTTATAAAGTGGGTCAGTAT                                           >  SRR4302537.4490054/1‑94 (MQ=60)
                                                    ACTTGTCTATAACAGTGAGCGGGTAAAAAATTATCAGCCTGCCCCTACCTATTATTCCGTGTCACAACAACTGGTTTATAAAGTGGGTCAGTAT                                           >  SRR4302537.2235808/1‑94 (MQ=60)
                                                     CTTGTCTATAACAGTGAGCGGGTAAAAAATTATCAGCCTGCCCCTACCTATTATTCCGTGTCACAACAACTGGTTTATAAAGTGGGTCAGTATC                                          >  SRR4302537.2350934/1‑94 (MQ=60)
                                                     CTTGTCTATAACAGTGAGCGGGTAAAAAATTATCAGCCTGCCCCTACCTATTATTCCGTGTCACAACAACTGGTTTATAAAGTGGGTCAGTATC                                          <  SRR4302537.2448771/94‑1 (MQ=60)
                                                     CTTCTCTAAAACAGTGAGCGGGTAAAAACTTATCAGCCTGCCCCTTCCTAGTATTCCGTGTCACAACAACTGGTTTATAAAGTGGGTCAGTATC                                          <  SRR4302537.1436353/94‑1 (MQ=60)
                                                      gttgCTATAACAGGTAGCGGGTAAAAAATTATCAGCCTGCCCATACCTATTATGCCGTGTCACAACAACTGGTTTATAAAGTGGGTCAGTATCG                                         <  SRR4302537.1430007/90‑1 (MQ=60)
                                                        GTCTATAACAGTGAGCGGGTAAAAAATTATCAGCCTGCCCCTACCTATTATTCCGTGTCACAACAACTGGTTTATAAAGTGGGTCAGTATCGCC                                       <  SRR4302537.6281482/94‑1 (MQ=60)
                                                         TCTATAACAGTGAGCGGGTAAAAAATTATCAGCCTGCCCCTACCTATTATTCCGTGTCACAACAACTGGTTTATAAAGTGGGTCAGTATCGCCC                                      >  SRR4302537.4941944/1‑94 (MQ=60)
                                                          CTATAACAGTGAGCGGGTAAAAAATTATCAGCCTGCCCCTACCTATTATTCCGTGTCACAACAACTGGTTTATAAAGTGGGTCAGTATCGCCCA                                     >  SRR4302537.1316998/1‑94 (MQ=60)
                                                          CTATAACAGTGAGCGGGTAAAAAATTATCAGCCTGCCCCTACCTATTATTCCGTGTCACAACAACTGGTTTATAAAGTGGGTCAGTATCGCCCA                                     >  SRR4302537.1936107/1‑94 (MQ=60)
                                                           TATAACAGTGAGCGGGTAAAAAATTATCAGCCTGCCCCTACCTATTATTCCGTGTCACAACAACTGGTTTATAAAGTGGGTCAGTATCGCCCAC                                    >  SRR4302537.174869/1‑94 (MQ=60)
                                                            ATAACAGTGAGCGGGTAAAAAATTATCAGCCTGCCCCTACCTATTATTCCGTGTCACAACAACTGGTTTATAAAGTGGGTCAGTATCGCCCACG                                   >  SRR4302537.5670577/1‑94 (MQ=60)
                                                             TAACAGTGAGCGGGTAAAAAATTATCAGCCTGCCCCTACCTATTATTCCGTGTCACAACAACTGGTTTATAAAGTGGGTCAGTATCGCCCACGT                                  <  SRR4302537.4690758/94‑1 (MQ=60)
                                                              AACAGTGAGCGGGTAAAAAATTATCAGCCTGCCCCTACCTATTATTCCGTGTCACAACAACTGGTTTATAAAGTGGGTCAGTATCGCCCACGTA                                 <  SRR4302537.4153374/94‑1 (MQ=60)
                                                               ACAGTGAGCGGGTAAAAAATTATCAGCCTGCCCCTACCTATTATTCCGTGTCACAACAACTGGTTTATAAAGTGGGTCAGTATCGCCCACGTAC                                <  SRR4302537.3636297/94‑1 (MQ=60)
                                                                CAGTGAGCGGGTAAAAAATTATCAGCCTGCCCCTACCTATTATTCCGTGTCACAACAACTGGTTTATAAAGTGGGTCAGTATCGCCCACGTACG                               >  SRR4302537.1397/1‑94 (MQ=60)
                                                                 AGTGAGCGGGTAAAAAATTATCAGCCTGCCCCTACCTATTATTCCGTGTCACAACAACTGGTTTATAAAGTGGGTCAGTATCGCCCACGTACGC                              >  SRR4302537.6367496/1‑94 (MQ=60)
                                                                 AGTGAGCGGGTAAAAAATTATCAGCCTGCCCCTACCTATTATTCCGTGTCACAACAACTGGTTTATAAAGTGGGTCAGTATCGCCCACGTACGC                              >  SRR4302537.4833913/1‑94 (MQ=60)
                                                                  GTGAGCGGGTAAAAAATTATCAGCCTGCCCCTACCTATTATTCCGTGTCACAACAACTGGTTTATAAAGTGGGTCAGTATCGCCCACGTACGCT                             >  SRR4302537.5244632/1‑94 (MQ=60)
                                                                   TGAGCGGGTAAAAAATTATCAGCCTGGCCCTACCTATTATTCCGTGTCACAACAACTGGTTTATAAAGTGGGTCAGTATCGCCCACGTACGCTG                            >  SRR4302537.1714116/1‑94 (MQ=60)
                                                                   TGAGCGGGTAAAAAATTATCAGCCTGCCCCTACCTATTATTCCGTGTCACAACAACTGGTTTATAAAGTGGGTCAGTATCGCCCACGTACGCTG                            >  SRR4302537.1000133/1‑94 (MQ=60)
                                                                    GAGCGGGTAAAAAATTATCAGCCTGCCCCTACCTATTATTCCGTGTCACAACAACTGGTTTATAAAGTGGGTCAGTATCGCCCACGTACGCTGG                           <  SRR4302537.551851/94‑1 (MQ=60)
                                                                     AGCGGGTAAAAAATTATCAGCCTGCCCCTACCTATTATTCCGTGTCACAACAACTGGTTTATAAAGTGGGTCAGTATCGCCCACGTACGCTGGG                          <  SRR4302537.1350707/94‑1 (MQ=60)
                                                                     AGCGGGTAAAAAATTATCAGCCTGCCCCTACCTATTATTCCGTGTCACAACAACTGGTTTATAAAGTGGGTCAGTATCGCCCACGTACGCTGGG                          >  SRR4302537.5229136/1‑94 (MQ=60)
                                                                       GGGGTAAAAAATTATCAGCCTGCCCCTACCTATTATTCCGTGTCACAACAACTGGTTTATAAAGTGGGTCAGTATCGCCCACGTACGCTGGGCA                        <  SRR4302537.1157782/94‑1 (MQ=60)
                                                                       CGGGTAAAAAATTATCAGCCTGCCCCTACCTATTATTCCGTGTCACAACAACTGGTTTATAAAGTGGGTCAGTATCGCCCACGTACGCTGGGCA                        <  SRR4302537.885236/94‑1 (MQ=60)
                                                                       CGGGTAAAAAATTATCAGCCTGCCCCTACCTATTATTCCGTGTCACAACAACTGGTTTATAAAGTGGGTCAGTATCGCCCACGTACGCTGGGCA                        <  SRR4302537.3953371/94‑1 (MQ=60)
                                                                       CGGGTAAAAAATTATCAGCCTGCCCCTACCTATTATTCCGTGTCACAACAACTGGTTTATAAAGTGGGTCAGTATCGCCCACGTACGCTGGGCA                        >  SRR4302537.847421/1‑94 (MQ=60)
                                                                        GGGTAAAAAATTATCAGCCTGCCCCTACCTATTATTCCGTGTCACAACAACTGGTTTATAAAGTGGGTCAGTATCGCCCACGTACGCTGGGCAA                       <  SRR4302537.490880/94‑1 (MQ=60)
                                                                         GGTAAAAAATTATCAGCCTGCCCCTACCTATTATTCCGTGTCACAACAACTGGTTTATAAAGTGGGTCAGTATCGCCCACGTACGCTGGGCAAC                      <  SRR4302537.6281174/94‑1 (MQ=60)
                                                                           TAAAAAATTATCAGCCTGCCCCTACCTATTATTCCGTGTCACAACAACTGGTTTATAAAGTGGGTCAGTATCGCCCACGTACGCTGGGCAACCT                    <  SRR4302537.5820510/94‑1 (MQ=60)
                                                                           TAAAAAATTATCAGCCTGCCCCTACCTATTATTCCGTGTCACAACAACTGGTTTATAAAGTGGGTCAGTATCGCCCACGTACGCTGGGCAACCT                    <  SRR4302537.2082751/94‑1 (MQ=60)
                                                                           TAAAAAATTATCAGCCTGCCCCTACCTATTATTCCGTGTCACAACAACTGGTTTATAAAGTGGGTCAGTATCGCCCACGTACGCTGGGCAACCT                    <  SRR4302537.5118268/94‑1 (MQ=60)
                                                                            AAAAAATTATCAGCCTGCCCCTACCTATTATTCCGTGTCACAACAACTGGTTTATAAAGTGGGTCAGTATCGCCCACGTACGCTGGGCAACCTG                   <  SRR4302537.2854727/94‑1 (MQ=60)
                                                                             AAAAATTATCAGCCTGCCCCTACCTATTATTCCGTGTCACAACAACTGGTTTATAAAGTGGGTCAGTATCGCCCACGTACGCTGGGCAACCTGA                  >  SRR4302537.4355647/1‑94 (MQ=60)
                                                                             AAAAATTATCAGCCTGCCCCTACCTATTATTCCGTGTCACAACAACTGGTTTATAAAGTGGGTCAGTATCGCCCACGTACGCTGGGCAACCTGA                  <  SRR4302537.4473399/94‑1 (MQ=60)
                                                                               AAATTATCAGCCTGCCCCTACCTATTATTCCGTGTCACAACAACTGGTTTATAAAGTGGGTCAGTATCGCCCACGTACGCTGGGCAACCTGACG                <  SRR4302537.808981/94‑1 (MQ=60)
                                                                               AAATTATCAGCCTGCCCCTACCTATTATTCCGTGTCACAACAACTGGTTTATAAAGTGGGTCAGTATCGCCCACGTACGCTGGGCAACCTGACG                >  SRR4302537.960117/1‑94 (MQ=60)
                                                                               AAATTATCAGCCTGCCCCTACCTATTATTCCGTGTCACAACAACTGGTTTATAAAGTGGGTCAGTATCGCCCACGTACGCTGGGCAACCTGACG                <  SRR4302537.5627582/94‑1 (MQ=60)
                                                                               AAATTATCAGCCTGCCCCTACCTATTATTCCGTGTCACAACAACTGGTTTATAAAGTGGGTCAGTATCGCCCACGTACGCTGGGCAACCTGACG                <  SRR4302537.5116399/94‑1 (MQ=60)
                                                                                AATTATCAGCCTGCCCCTACCTATTATTCCGTGTCACAACAACTGGTTTATAAAGTGGGTCAGTATCGCCCACGTACGCTGGGCAACCTGACGG               <  SRR4302537.3663439/94‑1 (MQ=60)
                                                                                AATTATCAGCCTGCCCCTACCTATTATTCCGTGTCACAACAACTGGTTTATAAAGTGGGTCAGTATCGCCCACGTACGCTGGGCAACCTGACGG               <  SRR4302537.1410178/94‑1 (MQ=60)
                                                                                 ATTATCAGCCTGCCCCTACCTATTATTCCGTGTCACAACAACTGGTTTATAAAGTGGGTCAGTATCGCCCACGTACGCTGGGCAACCTGACGGC              >  SRR4302537.2079383/1‑94 (MQ=60)
                                                                                  TTATCAGCCTGCCCCTACCTATTATTCCGTGTCACAACAACTGGTTTATAAAGTGGGTCAGTATCGCCCACGTACGCTGGGCAACCTGACGGCG             <  SRR4302537.1872952/94‑1 (MQ=60)
                                                                                    ATCAGCCTGGCCCTACCTATTATTCCGTGTCACAACAACTGGTTTATAAAGTGGGTCAGTATCGCCCACGTACGCTGGGCAACCTGACGGCGGA           >  SRR4302537.1662417/1‑94 (MQ=60)
                                                                                    ATCAGCCTGCCCCTACCTATTATTCCGTGTCACAACAACTGGTTTATAAAGTGGGTCAGTATCGCCCACGTACGCTGGGCAACCTGACGGCGGA           <  SRR4302537.1923764/94‑1 (MQ=60)
                                                                                    ATCAGCCTGCCCCTACCTATTATTCCGTGTCACAACAACTGGTTTATAAAGTGGGTCAGTATCGCCCACGTACGCTGGGCAACCTGACGGCGGA           >  SRR4302537.1183995/1‑94 (MQ=60)
                                                                                     TCAGCCTGGCCCTACCTATTATTCCGTGTCACAACAACTGGTTTATAAAGTGGGTCAGTATCGCCCACGTACGCTGGGCAACCTGACGGCGGAG          >  SRR4302537.5099572/1‑94 (MQ=60)
                                                                                      CAGCCTGCCCCTACCTATTATTCCGTGTCACAACAACTGGTTTATAAAGTGGGTCAGTATCGCCCACGTACGCTGGGCAACCTGACGGCGGAGC         >  SRR4302537.2069705/1‑94 (MQ=60)
                                                                                       AGCCTGCCCCTACCTATTATTCCGTGTCACAACAACTGGTTTATAAAGTGGGTCAGTATCGCCCACGTACGCTGGGCAACCTGACGGCGGAGCA        <  SRR4302537.5606323/94‑1 (MQ=60)
                                                                                         CCTGCCCCTACCTATTATTCCGTGTCACAACAACTGGTTTATAAAGTGGGTCAGTATCGCCCACGTACGCTGGGCAACCTGACGGCGGAGCAAC      <  SRR4302537.6000031/94‑1 (MQ=60)
                                                                                            GCCCCTACCTATTATTCCGTGTCACAACAACTGGTTTATAAAGTGGGTCAGTATCGCCCACGTACGCTGGGCAACCTGACGGCGGAGCAACTCA   >  SRR4302537.1574297/1‑94 (MQ=60)
                                                                                             CCCCTACCTATTATTCCGTGTCACAACAACTGGTTTATAAAGTGGGTCAGTATCGCCCACGTACGCTGGGCAACCTGACGGCGGAGCAACTCAC  >  SRR4302537.775221/1‑94 (MQ=60)
                                                                                             |                                                                                             
GCCAGCTGTTTGACGACCTTGATAATGGTAACGCCGACCTGCTGGCGGCAGGACTTGTCTATAACAGTGAGCGGGTAAAAAATTATCAGCCTGGCCCTACCTATTATTCCGTGTCACAACAACTGGTTTATAAAGTGGGTCAGTATCGCCCACGTACGCTGGGCAACCTGACGGCGGAGCAACTCAC  >  NC_000913/2694092‑2694278

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 2 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑