Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A1 F1 I2 R1	882	254.4	12957588	98.0%	12698436	93.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,722,352	(G)_5→6	coding (154/273 nt)	yfiM →	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,722,347	1	.	G	99.0%	662.4 / ‑0.9	194	coding (149/273 nt)	yfiM	hypothetical protein
Reads supporting (aligned to +/- strand): ref base . (1/1); new base G (107/85); total (108/86)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.12e-01

Only 100 of 215 total aligned reads displayed.

GATGCTTTCCGCCGCCGGAAATGAATATTCACAGCATCAGGGGATGAGCCGGGATCGCAGTGCCATGTTTGGATTGATGTTCTCTGTCAGTTT‑GGGGGCGTCAAAAGAGCTTTGGGATAGCCGCCCCGAAGGGAGCGGCTGGAGCTGGAAGGATTTGGCCTGGGATGTCGCCGGTGCAAGC  >  NC_000913/2722255‑2722435

gATGCTTTCCGCCGCCGGAAATGAATATTCACAGCATCAGGGGATGAGCCGGGATCGCAGTGCCATGTTTGGATTGATGTTCTCTGTCAGttt                                                                                           <  1:2893152/93‑1 (MQ=255)

gATGCTTTCCGCCGCCGGAAATGAATATTCACAGCATCAGGGGATGAGCCGGGATCGCAGTGCCATGTTTGGATTGATGTTCTCTGTCAGttt                                                                                           >  2:2441863/1‑93 (MQ=255)

 aTGCTTTCCGCCGCCGGAAATGAATATTCACAGCATCAGGGGATGAGCCGGGATCGCAGTGCCATGTTTGGATTGATGTTCTCTGTCAGTTT‑g                                                                                         <  2:2295698/93‑1 (MQ=255)

 aTGCTTTCCGCCGCCGGAAATGAATATTCACAGCATCAGGGGATGAGCCGGGATCGCAGTGCCATGGTTGGATTGATATTCTCTGTCTGTTT‑g                                                                                         >  1:5498660/1‑93 (MQ=255)

  tGCTTTCCGCAGCCGGAAATGAATATTCACAGCATCAGGGGATGAGCCGGGATCGCAGTGCCATGTTTGGATTGATGTTCTCTGTCAGTTT‑gg                                                                                        <  1:1065779/93‑1 (MQ=255)

   tcTTTCCGCCGCCGGAAATGAATATTCACAGCATCAGGGGATGAGCCGGGATCGCAGTGCCATGTTTGGATTGATGTTCTCTGTAAGTTT‑ggg                                                                                       <  2:1392230/92‑1 (MQ=255)

   gCTTTCCGCCGCCGGAAATGAATATTCACAGCATCAGGGGATGAGCCGGGATCGCAGTGCCATGTTTGGATTGATGTTCTCTGTCAGTTT‑ggg                                                                                       >  1:3752967/1‑93 (MQ=255)

   gCTTTCCGCCGCCGGAAATGAATATTCACAGCATCAGGGGATGAGCCGGGATCGCAGTGCCATGTTTGGATTGATGTTCTCTGTCAGTTT‑ggg                                                                                       <  2:3983036/93‑1 (MQ=255)

    cTTTCCGCCGCCGGAAATGAATATTCACAGCATCAGGGGATGAGCCGGGATCGCAGTGCCATGTTTGGATTGATGTTCTCTGTCAGTTT‑gggg                                                                                      >  2:1287795/1‑93 (MQ=255)

     tttCCGCCGCCGGAAATGAATATTCACAGCATCAGGGGATGAGCCGGGATCGCAGTGCCATGTTTGGATTGATGTTCTCTGTCAGTTT‑ggggg                                                                                     <  2:6343584/93‑1 (MQ=255)

           ccgccgGAAATGAATATTCACAGCATCAGGGGATGAGCCGGGATCGCAGTGCCATGTTTGGATTGATGTTCTCTGTCAGTTTGGGGGGCGTCa                                                                                >  1:742775/1‑93 (MQ=255)

             gccgGAAATGAATATTCACAGCATCAGGGGATGAGCCGGGATCGCAGTGCCATGTTTGGATTGATGTTCTCTGTCAGTTTGGGGGGCGTCaaa                                                                              >  2:154725/1‑93 (MQ=255)

              ccgGAAATGAATATTCACAGCATCAGGGGATGAGCCGGGATCGCAGTGCCATGTTTGGATTGATGTTCTCTGTCAGTTTGGGGGGCGTCaaaa                                                                             <  1:117324/93‑1 (MQ=255)

               cgGAAATGAATATTCACAGCATCAGGGGATGAGCCGGGATCGCAGTGCCATGTTTGGATTGATGTTCTCTGTCAGTTTGGGGGGCGTCAAAag                                                                            >  1:570197/1‑93 (MQ=255)

                ggAAATGAATATTCACAGCATCAGGGGATGAGCCGGGATCGCAGTGCCATGTTTGGATTGATGTTCTCTGTCAGTTTGGGGGGCGTCAAAaga                                                                           <  1:6173610/93‑1 (MQ=255)

                ggAAATGAATATTCACAGCATCAGGGGATGAGCCGGGATCGCAGTGCCATGTTTGGATTGATGTTCTCTGTCAGTTTGGGGGGCGTCAAAaga                                                                           <  2:6473120/93‑1 (MQ=255)

                 gAAATGAATATTCACAGCATCAGGGGATGAGCCGGGATCGCAGTGCCATGTTTGGATTGATGTTCTCTGTCAGTTTGGGGGGCGTCAAAagag                                                                          >  2:3545627/1‑93 (MQ=255)

                 gAAATGAATATTCACAGCATCAGGGGATGAGCCGGGATCGCAGTGCCATGTTTGGATTGATGTTCTCTGTCAGTTTGGGGGGCGTCAAAagag                                                                          >  2:294762/1‑93 (MQ=255)

                 gAAATGAATATTCACAGCATCAGGGGATGAGCCGGGATCGCAGTGCCATGTTTGGATTGATGTTCTCTGTCAGTTTGGGGGGCGTCAAAagag                                                                          >  1:4070331/1‑93 (MQ=255)

                   aaTGAATATTCACAGCATCAGGGGATGAGCCGGGATCGCAGTGCCATGTTTGGATTGATGTTCTCTGTCAGTTTGGGGGGCGTCAAAAGAGCt                                                                        >  1:185589/1‑93 (MQ=255)

                   aaTGAATATTCACAGCATCAGGGGATGAGCCGGGATCGCAGTGCCATGTTTGGATTGATGTTCTCTGTCAGTTTGGGGGGCGTCAAAAGAGCt                                                                        >  1:6384551/1‑93 (MQ=255)

                    aTGAATATTCACAGCATCAGGGGATGAGCCGGGATCGCAGTGCCATGTTTGGATTGATGTTCTCTGTCAGTTTGGGGGGCGTCAAAAGAGCtt                                                                       >  2:4446917/1‑93 (MQ=255)

                    aTGAATATTCACAGCATCAGGGGATGAGCCGGGATCGCAGTGCCATGTTTGGATTGATGTTCTCTGTCAGTTTGGGGGGCGTCAAAAGAGCtt                                                                       <  2:4231145/93‑1 (MQ=255)

                    aTGAATATTCACAGCATCAGGGGATGAGCCGGGATCGCAGTGCCATGTTTGGATTGATGTTCTCTGTCAGTTTGGGGGGCGTCAAAAGAGCtt                                                                       >  1:2579927/1‑93 (MQ=255)

                     tGAATATTCACAGCATCAGGGGATGAGCCGGGATCGCAGTGCCATGTTTGGATTGATGTTCTCTGTCAGTTTGGGGGGCGTCAAAAGAGCttt                                                                      <  1:1959953/93‑1 (MQ=255)

                      gAATATTCACAGCATCAGGGGATGAGCCGGGATCGCAGTGCCATGTTTGGATTGATGTTCTCTGTCAGTTTGGGGGGCGTCAAAAGAGCTTTg                                                                     <  1:2979492/93‑1 (MQ=255)

                      gAATATTCACAGCATCAGGGGATGAGCCGGGATCGCAGTGCCATGTTTGGATTGATGTTCTCTGTCAGTTTGGGGGGCGTCAAAAGAGCTTTg                                                                     >  1:788666/1‑93 (MQ=255)

                       aaTATTCACAGCATCAGGGGATGAGCCGGGATCGCAGTGCCATGTTTGGATTGATGTTCTCTGTCAGTTTGGGGGGCGTCAAAAGAGCTTTgg                                                                    <  1:4579368/93‑1 (MQ=255)

                         tatTCACAGCATCAGGGGATGAGCCGGGATCGCAGTGCCATGTTTGGATTGATGTTCTCTGTCAGTTTGGGGGGCGTCAAAAGAGCTTTGGGa                                                                  <  2:5196814/93‑1 (MQ=255)

                         tatTCACAGCATCAGGGGATGAGCCGGGATCGCAGTGCCATGTTTGGATTGATGTTCTCTGTCAGTTTGGGGGGCGTCAAAAGAGCTTTGGGa                                                                  <  1:1743614/93‑1 (MQ=255)

                         tatTCACAGCATCAGGGGATGAGCCGGGATCGCAGTGCCATGTTTGGATTGATGTTCTCTGTCAGTTTGGGGGGCGTCAAAAGAGCTTTGGGa                                                                  >  1:2323076/1‑93 (MQ=255)

                         tatTCACAGCATCAGGGGATGAGCCGGGATCGCAGTGCCATGTTTGGATTGATGTTCTCTGTCAGTTTGGGGGGCGTCAAAAGAGCTTTGGGa                                                                  <  2:5144284/93‑1 (MQ=255)

                         tatTCACAGCATCAGGGGATGAGCCGGGATCGCAGTGCCATGTTTGGATTGATGTTCTCTGTCAGTTTGGGGGGCGTCAAAAGAGCTTTGGGa                                                                  >  2:4186226/1‑93 (MQ=255)

                          atTCACAGCATCAGGGGATGAGCCGGGATCGCAGTGCCATGTTTGGATTGATGTTCTCTGTCAGTTTGGGGGGCGTCAAAAGAGCTTTGGGAt                                                                 <  2:2824628/93‑1 (MQ=255)

                           ttCACAGCATCAGGGGATGAGCCGGGATCGCAGTGCCATGTTTGGATTGATGTTCTCTGTCAGTTTGGGGGGCGTCAAAAGAGCTTTGGGATa                                                                <  1:5876515/93‑1 (MQ=255)

                           ttCACAGCATCAGGGGATGAGCCGGGATCGCAGTGCCATGTTTGGATTGATGTTCTCTGTCAGTTTGGGGGGCGTCAAAAGAGCTTTGGGATa                                                                >  1:6365324/1‑93 (MQ=255)

                              acaGCATCAGGGGATGAGCCGGGATCGCAGTGCCATGTTTGGATTGATGTTCTCTGTCAGTTTGGGGGGCGTCAAAAGAGCTTTGGGATAgcc                                                             <  2:2265795/93‑1 (MQ=255)

                              acaGAATCAGGGGATGAGCCGGGATCGCAGTGCCATGTTTGGATTGATGTTCTCTGTCAGTTTGGGGGGCGTCAAAAGAGCATTGGGATAgcc                                                             >  1:5203831/1‑93 (MQ=255)

                               caGCATCAGGGGATGAGCCGGGATCGCAGTGCCATGTTTGGATTGATGTTCTCTGTCAGTTTGGGGGGCGTCAAAAGAGCTTTGGGATAgccg                                                            >  2:1184904/1‑93 (MQ=255)

                                  cATCAGGGGATGAGCCGGGATCGCAGTGCCATGTTTGGATTGATGTTCTCTGTCAGTTTGGGGGGCGTCAAAAGAGCTTTGGGATAGCCGccc                                                         >  2:388049/1‑93 (MQ=255)

                                    tCAGGGGATGAGCCGGGATCGCAGTGCCATGTTTGGATTGATGTTCTCTGTCAGTTTGGGGGGCGTCAAAAGAGCTTTGGGATAGCCGCCCCg                                                       >  2:5276262/1‑93 (MQ=255)

                                      aGGGGATGAGCCGGGATCGCAGTGCCATGTTTGGATTGATGTTCTCTGTCAGTTTGGGGGGCGTCAAAAGAGCTTTGGGATAGCCGCCCCGaa                                                     >  2:6396587/1‑93 (MQ=255)

                                      aGGGGATGAGCCGGGATCGCAGTGCCATGTTTGGATTGATGTTCTCTGTCAGTTTGGGGGGCGTCAAAAGAGCTTTGGGATAGCCGCCCCGaa                                                     >  2:5132576/1‑93 (MQ=255)

                                       ggggATGAGCCGGGATCGCAGTGCCATGTTTGGATTGATGTTCTCTGTCAGTTTGGGGGGCGTCAAAAGAGCTTTGGGATAGCCGCCCCGAAg                                                    >  2:2432106/1‑93 (MQ=255)

                                       ggggATGAGCCGGGATCGCAGTGCCATGTTTGGATTGATGTTCTCTGTCAGTTTGGGGGGCGTCAAAAGAGCTTTGGGATAGCCGCCCCGAAg                                                    >  1:3150180/1‑93 (MQ=255)

                                        gggACGAGCCGGGATCGCAGTGCCATGTTTGGATTGATGTTCTCTGTCAGTTTGGGGGGCGTCAAAAGAGCTTTGGGATAGCCGCCCCGAAgg                                                   <  2:3211726/93‑1 (MQ=255)

                                         ggATGAGCCGGGATCGCAGTGCCATGTTTGGATTGATGTTCTCTGTCAGTTTGGGGGGCGTCAAAAGAGCTTTGGGATAGCCGCCCCGAAggg                                                  >  1:1691405/1‑93 (MQ=255)

                                          gATGAGCCGGGATCGCAGTGCCATGTTTGGATTGATGTTCTCTGTCAGTTTGGGGGGCGTCGAAAGAGCTTTGGGATAGCCGCCCCGAAGGGa                                                 <  2:5071993/93‑1 (MQ=255)

                                           aTGAGCCGGGATCGCAGTGCCATGTTTGGATTGATGTTCTCTGTCAGTTTGGGGGGCGTCAAAAGAGCTTTGGGATAGCCGCCCCGAAGGGAg                                                <  1:3322716/93‑1 (MQ=255)

                                           aTGAGCCGGGATCGCAGTGCCATGTTTGGATTGATGTTCTCTGTCAGTTTGGGGGGCGTCAAAAGAGCTTTGGGATAGCCGCCCCGAAGGGAg                                                <  2:4189309/93‑1 (MQ=255)

                                            tGAGCCGGGATCGCAGTGCCATGTTTGGATTGATGTTCTCTGTCAGTTTGGGGGGCGTCAAAAGAGCTTTGGGATAGCCGCCCCGAAGGGAGc                                               <  2:5430253/93‑1 (MQ=255)

                                            tGAGCCGGGATCGCAGTGCCATGTTTGGATTGATGTTCTCTGTCAGTTTGGGGGGCGTCAAAAGAGCTTTGGGATAGCCGCCCCGAAGGGAGc                                               >  2:4856714/1‑93 (MQ=255)

                                            tGAGCCGAGATCGCAGTGCCATGTTTGGATTGATGTTCTCTGTCAGTTTGGGGGGCGTCAAAAGAGCTTTGGGATAGCCGCCCCGAAGGGAGc                                               >  2:6139566/1‑93 (MQ=255)

                                             gAGCCGGGATCGCAGTGCCATGTTTGGATTGATGTTCTCTGTCAGTTTGGGGGGCGTCAAAAGAGCTTTGGGATAGCCGCCCCGAAGGGAGCg                                              <  1:5034046/93‑1 (MQ=255)

                                             gAGCCGGGATCGCAGTGCCATGTTTGGATTGATGTTCTCTGTCAGTTTGGGGGGCGTCAAAAGAGCTTTGGGATAGCCGCCCCGAAGGGAGCg                                              >  1:1253915/1‑93 (MQ=255)

                                               gCCGGGATCGCAGTGCCATGTTTGGATTTATGTTCTCTGTCAGTTTGGGGGGCGTCAAAAGAGCTTTGGGATAGCCGCCCCGAAGGGAGCGgc                                            <  1:2928786/93‑1 (MQ=255)

                                               gCCGGGATCGCAGTGCCATGTTTGGATTGATGTTCTCTGTCAGTTTGGGGGGCGTCAAAAGAGCTTTGGGATAGCCGCCCCGAAGGGAGCGgc                                            <  1:276570/93‑1 (MQ=255)

                                                   ggATCGCAGTGCCATGTTTGGATTGATGTTCTCTGTCAGTTTGGGGGGCGTCAAAAGAGCTTTGGGATAGCCGCCCCGAAGGGAGCGgctgga                                        >  1:3232921/1‑93 (MQ=255)

                                                    gATCGCAGTGCCATGTTTGGATTGATGTTCTCTGTCAGTTTGGGGGGCGTCAAAAGAGCTTTGGGATAGCCGCCCCGAAGGGAGCGgctggag                                       <  2:2086787/93‑1 (MQ=255)

                                                       cGCAGTGCCATGTTTGGATTGATGTTCTCTGTCAGTTTGGGGGGCGTCAAAAGAGCTTTGGGATAGCCGCCCCGAAGGGAGCGgctggagctg                                    <  1:2029261/93‑1 (MQ=255)

                                                       cGCAGTGCCATGTTTGGATTGATGTTCTCTGTCAGTTTGGGGGGCGTCAAAAGAGCTTTGGGATAGCCGCCCCGAAGGGAGCGgctggagctg                                    >  2:3287429/1‑93 (MQ=255)

                                                        gCAGTGCCATGTTTGGATTGATGTTCTCTGTCAGTTTGGGGGGCGTCAAAAGAGCTTTGGGATAGCCGCCCCGAAGGGAGCGgctggagctgg                                   <  1:2127197/93‑1 (MQ=255)

                                                         cAGTGCCATGTTTGGATTGATGTTCTCTGTCAGTTTGGGGGGCGTCAAAAGAGCTTTGGGATAGCCGCCCCGAAGGGAGCGgctggagctgga                                  <  2:1009156/93‑1 (MQ=255)

                                                         cAGTGCCATGTTTGGATTGATGTTCTCTGTCAGTTTGGGGGGCGTCAAAAGAGCTTTGGGATAGCCGCCCCGAAGGGAGCGgctggagctgga                                  <  1:3870712/93‑1 (MQ=255)

                                                          aGTGCCATGTTTGGATTGATGTTCTCTGTCAGTTTGGGGGGCGTCAAAAGAGCTTTGGGATAGCCGCCCCGAAGGGAGCGGCTGGAGCTGGaa                                 <  2:6082741/93‑1 (MQ=255)

                                                          aGTGCCATGTTTGGATTGATGTTCTCTGTCAGTTTGGGGGGCGTCAAAAGAGCTTTGGGATAGCCGCCCCGAAGGGAGCGGCTGGAGCTGGaa                                 <  2:5877776/93‑1 (MQ=255)

                                                          aGTGCCATGTTTGGATTGATGTTCTCTGTCAGTTTGGGGGGCATCAAAAGAGCTTTGGGATAGCCGCCCCGAAGGGAGCGGCTGGAGCTGGaa                                 >  2:4965400/1‑93 (MQ=255)

                                                              ccATGTTTGGATTGATGTTCTCTGTCAGTTTGGGGGGCGTCAAAAGAGCTTTGGGATAGCCGCCCCGAAGGGAGCGGCTGGAGCTGGAAGGAt                             >  2:1663035/1‑93 (MQ=255)

                                                              ccATGTTTGGATTGATGTTCTCTGTCAGTTTGGGGGGCGTCAAAAGAGCTTTGGGATAGCCGCCCCGAAGGGAGCGGCTGGAGCTGGAAGGAt                             >  2:6032987/1‑93 (MQ=255)

                                                              ccATGTTTGGATTGATGTTCTCTGTCAGTTTGGGGGGCGTCAAAAGAGCTTTGGGATAGCCGCCCCGAAGGGAGCGGCTGGAGCTGGAAGGAt                             >  1:5820736/1‑93 (MQ=255)

                                                              ccATGTTTGGATTGATGTTCTCTGTCAGTTTGGGGGGCGTCAAAAGAGCTTTGGGATAGCCGCCCCGAAGGGAGCGGCTGGAGCTGGAAGGAt                             >  2:5661414/1‑93 (MQ=255)

                                                               cATGTTTGGATTGATGTTCTCTGTCAGTTTGGGGGGCGTCAAAAGAGCTTTGGGATAGCCGCCCCGAAGGGAGCGGCTGGAGCTGGAAGGAtt                            <  2:69724/93‑1 (MQ=255)

                                                                aTGTTTGGATTGATGTTCTCTGTCAGTTTGGGGGGCGTCAAAAGAGCTTTGGGATAGCCGCCCCGAAGGGAGCGGCTGGAGCTGGAAGGAttt                           >  1:2915914/1‑93 (MQ=255)

                                                                 tGTTTGGATTGATGTTCTCTGTCAGTTTGGGGGGCGTCAAAAGAGCTTTGGGATAGCCGCCCCGAAGGGAGCGGCTGGAGCTGGAAGGATTTg                          <  2:573721/93‑1 (MQ=255)

                                                                 tGTTTGGATTGATGTTCTCTGTCAGTTTGGGGGGCGTCAAAAGAGCTTTGGGATAGCCGCCCCGAAGGGAGCGGCTGGAGCTGGAAGGATTTg                          >  1:3035330/1‑93 (MQ=255)

                                                                  gTTTGGATTGATGTTCTCTGTCAGTTTGGGGGGCGTCAAAAGAGCTTTGGGATAGCCGCCCCGAAGGGAGCGGCTGGAGCTGGAAGGATTTgg                         >  1:2629783/1‑93 (MQ=255)

                                                                   tttGGATTGATGTTCTCTGTCAGTTTGGGGGGCGTCAAAAGAGCTTTGGGATAGCCGCCCCGAAGGGAGCGGCTGGAGCTGGAAGGATTTGGc                        >  1:1529003/1‑93 (MQ=255)

                                                                    ttGGATTGATGTTCTCTGTCAGTTTGGGGGGCGTCAAAAGAGCTTTGGGATAGCCGCCCCGAAGGGAGCGGCTGGAGCTGGAAGGATTTGGcc                       <  2:747392/93‑1 (MQ=255)

                                                                      ggATTGATGTTCTCTGTCAGTTTGGGGGGCGTCAAAAGAGCTTTGGGATAGCCGCCCCGAAGGGAGCGGCTGGAGCTGGAAGGATTTGGCCTg                     <  2:4667840/93‑1 (MQ=255)

                                                                      ggATTGATGTTCTCTGTCAGTTTGGGGGGCGTCAAAAGAGCTTTGGGATAGCCGCCCCGAAGGGAGCGGCTGGAGCTGGAAGGATTTGGCCTg                     >  1:2011553/1‑93 (MQ=255)

                                                                      ggATTGATGTTCTCTGTCAGTTTGGGGGGCGTCAAAAGAGCTTTGGGATAGCCGCCCCGAAGGGAGCGGCTGGAGCTGGAAGGATTTGGCCTg                     <  1:275635/93‑1 (MQ=255)

                                                                       gATTGATGTTCTCTGTCAGTTTGGGGGGCGTCAAAAGAGCTTTGGGATAGCCGCCCCGAAGGGAGCGGCTGGAGCTGGAAGGATTTGGCCTgg                    >  2:1808995/1‑93 (MQ=255)

                                                                         ttGATGTTCTCTGTCAGTTTGGGGGGCGTCAAAAGAGCTTTGGGATAGCCGCCCCGAAGGGAGCGGCTGGAGCTGGAAGGATTTGGCCTGGGa                  >  1:4291009/1‑93 (MQ=255)

                                                                           gATGTTCTCTGTCAGTTTGGGGGGCGTCAAAAGAGCTTTGGGATAGCCGCCCCGAAGGGAGCGGCTGGAGCTGGAAGGATTTGGCCTGGGATg                >  1:413357/1‑93 (MQ=255)

                                                                            aTGTTCTCTGTCAGTTTGGGGGGCGTCAAAAGAGCTTTGGGATAGCCGCCCCGAAGGGAGCGGCTGGAGCTGGAAGGATTTGGCCTGGGATGt               <  1:4648240/93‑1 (MQ=255)

                                                                             tGTTCTCTGTCAGTTTGGGGGGCGTCAAAAGAGCTTTGGGATAGCCGCCCCGAAGGGAGCGGCTGGAGCTGGAAGGATTTGGCCTGGGATGTc              >  1:4817198/1‑93 (MQ=255)

                                                                              gTTCTCTGTCAGTTTGGGGGGCGTCAAAAGAGCTTTGGGATAGCCGCCCCGAAGGGAGCGGCTGGAGCTGGAAGGATTTGGCCTGGGATGTCg             >  2:3748872/1‑93 (MQ=255)

                                                                                tctcTGTCAGTTTGGGGGGCGTCAAAAGAGCTTTGGGATAGCCGCCCCGAAGGGAGCGGCTGGAGCTGGAAGGATTTGGCCTGGGATGTCGcc           >  1:2512264/1‑93 (MQ=255)

                                                                                tctcTGTCAGTTTGGGGGGCGTCAAAAGAGCTTTGGGATAGCCGCCCCGAAGGGAGCGGCTGGAGCTGGAAGGATTTGGCCTGGGATGTCGcc           <  2:1152133/93‑1 (MQ=255)

                                                                                 ctctGTCAGTTTGGGGGGCGTCAAAAGAGCTTTGGGATAGCCGCCCCGAAGGGAGCGGCTGGAGTTGGAAGGATTTGGCCTGGGATGTCGCCg          >  2:4290609/1‑93 (MQ=255)

                                                                                 ctctGTCAGTTTGGGGGGCGTCAAAAGAGCTTTGGGATAGCCGCCCCGAAGGGAGCGGCTGGAGCTGGAAGGATTTGGCCTGGGATGTCGCCg          >  1:2064803/1‑93 (MQ=255)

                                                                                  tctGTCAGTTTGGGGGGCGTCAAAAGAGCTTTGGGATAGCCGCCCCGAAGGGAGCGGCTGGAGCTGGAAGGATTTGGCCTGGGATGTCGCCgg         >  2:3863492/1‑93 (MQ=255)

                                                                                   ctGTCAGTTTGGGGGGCGTCAAAAGAGCTTTGGGATAGCCGCCCCGAAGGGAGCGGCTGGAGCTGGAAGGATTTGGCCTGGGATGTCGCCGGt        <  1:3809081/93‑1 (MQ=255)

                                                                                    tGTCAGTTTGGGGGGCGTCAAAAGAGCTTTGGGATAGCCGCCCCGAAGGGAGCGGCTGGAGCTGGAAGGATTTGGCCTGGGATGTCGGCGGTg       >  1:3369028/1‑93 (MQ=255)

                                                                                    tGTCAGTTTGGGGGGCGTCAAAAGAGCTTTGGGATAGCCGCCCCGAAGGGAGCGGCTGGAGCTGGAAGGATTTGGCCTGGGATGTCGCCGGTg       >  2:2357899/1‑93 (MQ=255)

                                                                                     gTCAGTTTGGGGGGCGTCAAAAGAGCTTTGGGATAGCCGCCCCGAAGGGAGCGGCTGGAGCTGGAAGGATTTGGCCTGGGATGTCGCCGGTGc      >  1:1324422/1‑93 (MQ=255)

                                                                                      tCAGTTTGGGGGGCGTCAAAAGAGCTTTGGGATAGCCGCCCCGAAGGGAGCGGCTGGAGCTGGAAGGATTTGGCCTGGGATGTCGCCGGTGCa     >  1:6469534/1‑93 (MQ=255)

                                                                                       cAGTTTGGGGGGCGTCAAAAGAGCTTTGGGATAGCCGCCCCGAAGGGAGCGGCTGGAGCTGGAAGGATTTGGCCTGGGATGTCGCCGGTGCaa    <  2:5953013/93‑1 (MQ=255)

                                                                                       cAGTTTGGGGGGCGTCAAAAGAGCTTGGGGATAGCCGCCCCGAAGGGAGCGGCTGGAGCTGGAAGGATTTGGCCTGGGATGTCGCCGGTGCaa    <  2:1704504/93‑1 (MQ=255)

                                                                                        gtttt‑GGGGGCGTCAAAAGAGCTTTGGGATAGCCGCCCCGAAGGGAGCGGCTGGAGCTGGAAGGATTTGGCCTGGGATGTCGCCGGTGCAAGc  >  1:400193/3‑93 (MQ=255)

GATGCTTTCCGCCGCCGGAAATGAATATTCACAGCATCAGGGGATGAGCCGGGATCGCAGTGCCATGTTTGGATTGATGTTCTCTGTCAGTTT‑GGGGGCGTCAAAAGAGCTTTGGGATAGCCGCCCCGAAGGGAGCGGCTGGAGCTGGAAGGATTTGGCCTGGGATGTCGCCGGTGCAAGC  >  NC_000913/2722255‑2722435

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 21 ≤` `ATCG/ATCG` `< 29 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

BRESEQ :: bam2aln output

CTTTCCGCCGCCGGAAATGAATATTCACAGCATCAGGGGATGAGCCGGGATCGCAGTGCCATGTTTGGATTGATGTTCTCTGTCAGTTT‑GGGGGCGTCAAAAGAGCTTTGGGATAGCCGCCCCGAAGGGAGCGGCTGGAGCTGGAAGGATTTGGCCTGGGATGTCGCCGGTGCAAGCACCGGCT  >  NC_000913/2722259‑2722442

CTTTCCGCCGCCGGAAATGAATATTCACAGCATCAGGGGATGAGCCGGAATCGCAGTGCCATGTTTGGATTGATGTTCTCTGTCAGTTT‑GGGGG                                                                                            >  SRR4302537.3753260/1‑94 (MQ=60)

 TTTCCGCCGCCGGAAATGAATATTCACAGCATCAGGGGATGAGCCGGGATCGCAGTGCCATGTTTGGATTGATGTTCTCTGTCAGTTT‑GGGGGG                                                                                           <  SRR4302537.2108506/94‑1 (MQ=60)

     CGCCGCCGGAAATGAATATTCACAGCATCAGGGGATGAGCCGGGATCGCAGTGCCATGTTTGGATTGATGTTCTCTGTCAGTTTGGGGGGCGTC                                                                                        >  SRR4302537.2948915/1‑94 (MQ=60)

      GCCGCCGGAAATGAATATTCACAGCATCAGGGGATGAGCCGGGATCGCAGTGCCATGTTTGGATTGATGTTCTCTGTCAGTTTGGGGGGCGTCA                                                                                       >  SRR4302537.747840/1‑94 (MQ=60)

          CCGGAAATGAATATTCACAGCATCAGGGGATGAGCCGGGATCGCAGTGCCATGTTTGGATTGATGTTCTCTGTCAGTTTGGGGGGCGTCAAAAG                                                                                   >  SRR4302537.574113/1‑94 (MQ=60)

          CCGGAAATGAATATTCACAGCATCAGGGGATGAGCCGGGATCGCAGTGCCATGTTTGGATTGATGTTCTCTGTCAGTTTGGGGGGCGTCAAAAG                                                                                   >  SRR4302537.6086038/1‑94 (MQ=60)

          CCGGAAATGAATATTCACAGCATCAGGGGATGAGCCGGGATCGCAGTGCCATGTTTGGATTGATGTTCTCTGTCAGTTTGGGGGGCGTCAAAAG                                                                                   <  SRR4302537.6485483/94‑1 (MQ=60)

          CCGGAAATGAATATTCACAGCATCAGGGGATGAGCCGGGATCGCAGTGCCATGTTTGGATTGATGTTCTCTGTCAGTTTGGGGGGCGTCAAAAG                                                                                   <  SRR4302537.118170/94‑1 (MQ=60)

            GGAAATGAATATTCACAGCATCAGGGGATGAGCCGGGATCGCAGTGCCATGTTTGGCTTGATGTTCTCTGTCAGTTTGGGGGGCGTCAAAAGAG                                                                                 >  SRR4302537.159292/1‑94 (MQ=60)

            GGAAATGAATATTCACAGCATCAGGGGATGAGCCGGGATCGCAGTGCCATGTTTGGATTGATGTTCTCTGTCAGTTTGGGGGGCGTCAAAAGAG                                                                                 >  SRR4302537.4687912/1‑94 (MQ=60)

            GGAAATGAATATTCACAGCATCAGGGGATGAGCCGGGATCGCAGTGCCATGTTTGGATTGATGTTCTCTGTCAGTTTGGGGGGCGTCAAAAGAG                                                                                 >  SRR4302537.5863349/1‑94 (MQ=60)

            GGAAATGAATATTCACAGCATCAGGGGATGAGCCGGGATCGCAGTGCCATGTTTGGATTGATGTTCTCTGTCAGTTTGGGGGGCGTCAAAAGAG                                                                                 <  SRR4302537.6216130/94‑1 (MQ=60)

            GGAAATGAATATTCACAGCATCAGGGGATGAGCCGGGATCGCAGTGCCATGTTTGGATTGATGTTCTCTGTCAGTTTGGGGGGCGTCAAAAGAG                                                                                 >  SRR4302537.4098757/1‑94 (MQ=60)

              AAATGAATATTCACAGCATCAGGGGATGAGCCGGGATCGCAGTGCCATGTTTGGATTGATGTTCTCTGTCAGTTTGGGGGGCGTCAAAAGAGCT                                                                               <  SRR4302537.3964926/94‑1 (MQ=60)

              AAATGAATATTCACAGCATCAGGGGATGAGCCGGGATCGCAGTGCCATGTTTGGATTGATGTTCTCTGTCAGTTTGGGGGGCGTCAAAAGAGCT                                                                               >  SRR4302537.6428067/1‑94 (MQ=60)

              AAATGAATATTCACAGCATCAGGGGATGAGCCGGGATCGCAGTGCCATGTTTGGATTGATGTTCTCTGTCAGTTTGGGGGGCGTCAAAAGAGCT                                                                               >  SRR4302537.186910/1‑94 (MQ=60)

               AATGAATATTCACAGCATCAGGGGATGAGCCGGGATCGCAGTGCCATGTTTGGATTGATGTTCTCTGTCAGTTTGGGGGGCGTCAAAAGAGCTT                                                                              >  SRR4302537.2597986/1‑94 (MQ=60)

               AATGAATATTCACAGCATCAGGGGATGAGCCGGGATCGCAGTGCCATGTTTGGATTGATGTTCTCTGTCAGTTTGGGGGGCGTCAAAAGAGCTT                                                                              >  SRR4302537.2708200/1‑94 (MQ=60)

                 TGAATATTCACAGCATCAGGGGATGAGCCGGGATCGCAGTGCCATGTTTGGATTGATGTTCTCTGTCAGTTTGGGGGGCGTCAAAAGAGCTTTG                                                                            >  SRR4302537.794082/1‑94 (MQ=60)

                 TGAATATTCACAGCATCAGGGGATGAGCCGGGATCGCAGTGCCATGTTTGGATTGATGTTCTCTGTCAGTTTGGGGGGCGTCAAAAGAGCTTTG                                                                            >  SRR4302537.2630688/1‑94 (MQ=60)

                 TGAATATTCACAGCATCAGGGGATGAGCCGGGATCGCAGTGCCATGTTTGGATTGATGTTCTCTGTCAGTTTGGGGGGCGTCAAAAGAGCTTTG                                                                            <  SRR4302537.1973586/94‑1 (MQ=60)

                  GAATATTCACAGCATCAGGGGATGAGCCGGGATCGCAGTGCCATGTTTGGATTGATGTTCTCTGTCAGTTTGGGGGGCGTCAAAAGAGCTTTGG                                                                           >  SRR4302537.675580/1‑94 (MQ=60)

                  GAATATTCACAGCATCAGGGGATGAGCCGGGATCGCAGTGCCATGTTTGGATTGATGTTCTCTGTCAGTTTGGGGGGCGTCAAAAGAGCTTTGG                                                                           <  SRR4302537.3000334/94‑1 (MQ=60)

                   AATATTCACAGCATCAGGGGATGAGCCGGGATCGCAGTGCCATGTTTGGATTGATGTTCTCTGTCAGTTTGGGGGGCGTCAAAAGAGCTTTGGG                                                                          <  SRR4302537.4677472/94‑1 (MQ=60)

                   AATATTCACAGCATCAGGGGATGAGCCGGGATCGCAGTGCCATGTTTGGATTGATGTTCTCTGTCAGTTTGGGGGGCGTCAAAAGAGCTTTGGG                                                                          <  SRR4302537.4611050/94‑1 (MQ=60)

                    ATATTCACAGCATCAGGGGATGAGCCGGGATCGCAGTGCCATGTTTGGATTGATGTTCTCTGTCAGTTTGGGGGGCGTCAAAAGAGCTTTGGGA                                                                         <  SRR4302537.2245001/94‑1 (MQ=60)

                    ATATTCACAGCATCAGGGGATGAGCCGGGATCGCAGTGCCATGTTTGGATTGATGTTCTCTGTCAGTTTGGGGGGCGTCAAAAGAGCTTTGGGA                                                                         >  SRR4302537.2339476/1‑94 (MQ=60)

                     TATTCACAGCATCAGGGGATGAGCCGGGATCGCAGTGCCATGTTTGGATTGATGTTCTCTGTCAGTTTGGGGGGCGTCAAAAGAGCTTTGGGAT                                                                        <  SRR4302537.1755769/94‑1 (MQ=60)

                     TATTCACAGCATCAGGGGATGAGCCGGGATCGCAGTGCCATGTTTGGATTGATGTTCTCTGTCAGTTTGGGGGGCGTCAAAAGAGCTTTGGGAT                                                                        >  SRR4302537.772320/1‑94 (MQ=60)

                      ATTCACAGCATCAGGGGATGAGCCGGGATCGCAGTGCCATGTTTGGATTGATGTTCTCTGTCAGTTTGGGGGGCGTCAAAAGAGCTTTGGGATA                                                                       >  SRR4302537.6408739/1‑94 (MQ=60)

                      ATTCACAGCATCAGGGGATGAGCCGGGATCGCAGTGCCATGTTTGGATTGATGTTCTCTGTCAGTTTGGGGGGCGTCAAAAGAGCTTTGGGATA                                                                       <  SRR4302537.1493649/94‑1 (MQ=60)

                       TTCACAGCATCAGGGGATGAGCCGGGATCGCAGTGCCATGTTTGGATTGATGTTCTCTGTCAGTTTGGGGGGCGTCAAAAGAGCTTTGGGATAG                                                                      <  SRR4302537.5916834/94‑1 (MQ=60)

                        TCACAGCATCAGGGGATGAGCCGGGATCGCAGTGCCATGTTTGGATTGATGTTCTCTGTCAGTTTGGGGGGCGTCAAAAGAGCTTTGGGATAGC                                                                     <  SRR4302537.1253929/94‑1 (MQ=60)

                        TCACAGCATCAGGGGATGAGCCGGGATCGCAGTGCCATGTTTGGATTGATGTTCTCTGTCAGTTTGGGGGGCGTCAAAAGAGCTTTGGGATAGC                                                                     <  SRR4302537.4320007/94‑1 (MQ=60)

                        TCACAGCATCAGGGGATGAGCCGGGATCGCAGTGCCATGTTTGGATTGATGTTCTCTGTCAGTTTGGGGGGCGTCAAAAGAGCTTTGGGATAGC                                                                     <  SRR4302537.3034405/94‑1 (MQ=60)

                         CACAGAATCAGGGGATGAGCCGGGATCGCAGTGCCATGTTTGGATTGATGTTCTCTGTCAGTTTGGGGGGCGTCAAAAGAGCATTGGGATAGCC                                                                    >  SRR4302537.5239626/1‑94 (MQ=60)

                          ACAGCATCAGGGGATGAGCCGGGATCGCAGTGCCATGTTTGGATTGATGTTCTCTGTCAGTTTGGGGGGCGTCAAAAGAGCTTTGGGATAGCCG                                                                   <  SRR4302537.2925643/94‑1 (MQ=60)

                              CATCAGGGGATGAGCCGGGATCGCAGTGCCATGTTTGGATTGATGTTCTCTGTCAGTTTGGGGGGCGTCAAAAGAGCTTTGGGATAGCCGCCCC                                                               <  SRR4302537.4372677/94‑1 (MQ=60)

                              CATCAGGGGATGAGCCGGGATCGCAGTGCCATGTTTGGATTGATGTTCTCTGTCAGTTTGGGGGGCGTCAAAAGAGCTTTGGGATAGCCGCCCC                                                               >  SRR4302537.4049871/1‑94 (MQ=60)

                               ATCAGGGGATGAGCCGGGATCGCAGTGCCATGTTTGGATTGATGTTCTCTGTCAGTTTGGGGGGCGTCAAAAGAGCTTTGGGATAGCCGCCCCG                                                              >  SRR4302537.5233327/1‑94 (MQ=60)

                               ATCAGGGGATGAGCCGGGATCGCAGTGCCATGTTTGGATTGATGTTCTCTGTCAGTTTGGGGGGCGTCAAAAGAGCTTTGGGATAGCCGCCCCG                                                              <  SRR4302537.3292042/94‑1 (MQ=60)

                                  AGGGGATGAGCCGGGATCGCAGTGCCATGTTTGGATTGATGTTCTCTGTCAGTTTGGGGGGCGTCAAAAGAGCTTTGGGATAGCCGCCCCGAAG                                                           >  SRR4302537.2408695/1‑94 (MQ=60)

                                  AGGGGATGAGCCGGGATCGCAGTGCCATGTTTGGATTGATGTTCTCTGTCAGTTTGGGGGGCGTCAAAAGAGCTTTGGGATAGCCGCCCCGAAG                                                           >  SRR4302537.2926298/1‑94 (MQ=60)

                                  AGGGGATGAGCCGGGATCGCAGTGCCATGTTTGGATTGATGTTCTCTGTCAGTTTGGGGGGCGTCAAAAGAGCTTTGGGATAGCCGCCCCGAAG                                                           >  SRR4302537.3172270/1‑94 (MQ=60)

                                   GGGGATGAGCCGGGATCGCAGTGCCATGTTTGGATTGATGTTCTCTGTCAGTTTGGGGGGCGTCAAAAGAGCTTTGGGATAGCCGCCCCGAAGG                                                          <  SRR4302537.3185693/94‑1 (MQ=60)

                                    GGGATGAGCCGGGATCGCAGTGCCATGTTTGGATTGATGTTCTCTGTCAGTTTGGGGGGCGTCAAAAGAGCTTTGGGATAGCCGCCCCGAAGGG                                                         >  SRR4302537.1703245/1‑94 (MQ=60)

                                       ATGAGCCGGGATCGCAGTGCCATGTTTGGATTGATGTTCTCTGTCAGTTTGGGGGGCGTCAAAAGAGCTTTGGGATAGCCGCCCCGAAGGGAGC                                                      <  SRR4302537.3346222/94‑1 (MQ=60)

                                        TGAGCCGGGATCGCAGTGCCATGTTTGGATTGATGTTCTCTGTCAGTTTGGGGGGCGTCAAAAGAGCTTTGGGATAGCCGCCCCGAAGGGAGCG                                                     <  SRR4302537.3384565/94‑1 (MQ=60)

                                        TGAGCCGGGATCGCAGTGCCATGTTTGGATTGATGTTCTCTGTCAGTTTGGGGGGCGTCAAAAGAGCTTTGGGATAGCCGCCCCGAAGGGAGCG                                                     >  SRR4302537.1262639/1‑94 (MQ=60)

                                         GAGCCGGGATCGCAGTGCCATGTTTGGATTGATGTTCTCTGTCAGTTTGGGGGGCGTCAAAAGAGCTTTGGGATAGCCGCCCCGAAGGGAGCGG                                                    <  SRR4302537.5068702/94‑1 (MQ=60)

                                           GCCGGGATCGCAGTGCCATGTTTGGATTTATGTTCTCTGTCAGTTTGGGGGGCGTCAAAAGAGCTTTGGGATAGCCGCCCCGAAGGGAGCGGCT                                                  <  SRR4302537.2949354/94‑1 (MQ=60)

                                           GCCGGGATCGCAGTGCCATGTTTGGATTGATGTTCTCTGTCAGTTTGGGGGGCGTCAAAAGAGCTTTGGGATAGCCGCCCCGAAGGGAGCGGCT                                                  <  SRR4302537.278504/94‑1 (MQ=60)

                                            CCGGGATCGCAGTGCCATGTTTGGATTGATGTTCTCTGTCAGTTTGGGGGGCGTCAAAAGAGCTTTGGGATAGCCGCCCCGAAGGGAGCGGCTG                                                 >  SRR4302537.6486965/1‑94 (MQ=60)

                                             CGGGATCGCAGTGCCATGTTTGGATTGATGTTCTCTGTCAGTTTGGGGGGCGTCAAAAGAGCTTTGGGATAGCCGCCCCGAAGGGAGCGGCTGG                                                <  SRR4302537.2050274/94‑1 (MQ=60)

                                              GGGATCGCAGTGCCATGTTTGGATTGATGTTCTCTGTCAGTTTGGGGGGCGTCAAAAGAGCTTTGGGATAGCCGCCCCGAAGGGAGCGGCTGGA                                               >  SRR4302537.3255701/1‑94 (MQ=60)

                                                 ATCGCAGTGCCATGTTTGGATTGATGTTCTCTGTCAGTTTGGGGGGCGTCAAAAGAGCTTTGGGATAGCCGCCCCGCAGGGAGCGGCTGGCGCT                                            >  SRR4302537.5784331/1‑94 (MQ=60)

                                                   CGCAGTGCCATGTTTGGATTGATGTTCTCTGTCAGTTTGGGGGGCGTCAAAAGAGCTTTGGGATAGCCGCCCCGAAGGGAGCGGCTGGAGCTGG                                          >  SRR4302537.1167227/1‑94 (MQ=60)

                                                   CGCAGTGCCATGTTTGGATTGATGTTCTCTGTCAGTTTGGGGGGCGTCAAAAGAGCTTTGGGATAGCCGCCCCGAAGGGAGCGGCTGGAGCTGG                                          >  SRR4302537.2776920/1‑94 (MQ=60)

                                                   CGCAGTGCCATGTTTGGATTGATGTTCTCTGTCAGTTTGGGGGGCGTCAAAAGAGCTTTGGGATAGCCGCCCCGAAGGGAGCGGCTGGAGCTGG                                          <  SRR4302537.2043409/94‑1 (MQ=60)

                                                    GCAGTGCCATGTTTGGATTGATGTTCTCTGTCAGTTTGGGGGGCGTCAAAAGAGCTTTGGGATAGCCGCCCCGAAGGGAGCGGCTGGAGCTGGA                                         <  SRR4302537.2142158/94‑1 (MQ=60)

                                                    GCAGTGCCATGTTTGGATTGATGTTCTCTGTCAGTTTGGGGGGCGTCAAAAGAGCTTTGGGATAGCCGCCCCGAAGGGAGCGGCTGGAGCTGGA                                         >  SRR4302537.3388418/1‑94 (MQ=60)

                                                     CAGTGCCATGTTTGGATTGATGTTCTCTGTCAGTTTGGGGGGCGTCAAAAGAGCTTTGGGATAGCCGCCCCGAAGGGAGCGGCTGGAGCTGGAA                                        <  SRR4302537.5328570/94‑1 (MQ=60)

                                                     CAGTGCCATGTTTGGATTGATGTTCTCTGTCAGTTTGGGGGGCGTCAAAAGAGCTTTGGGATAGCCGCCCCGAAGGGAGCGGCTGGAGCTGGAA                                        <  SRR4302537.3897797/94‑1 (MQ=60)

                                                      AGTTCTATGTTTGGATTGATGTTCTCTGTCAGTTTGGGGGGCGTCAAAAGAGCTTTGGGATAGCCGCCCCGAAGGGAGCGGCTGGAGCTGGAAG                                       <  SRR4302537.4468508/94‑1 (MQ=60)

                                                      AGTGCCATGTTTGGATTGATGTTCTCTGTCAGTTTGGGGGGCGTCAAAAGAGCTTTGGGATAGCCGCCCCGAAGGGAGCGGCTGGAGCTGGAAG                                       >  SRR4302537.6297624/1‑94 (MQ=60)

                                                       GTGCCATGTTTGGATTGATGTTCTCTGTCAGTTTGGGGGGCGTCAAAAGAGCTTTGGGATAGCCGCCCCGAAGGGAGCGGCTGGAGCTGGAAGG                                      >  SRR4302537.3797772/1‑94 (MQ=60)

                                                        TGCCATGTTTGGATTGATGTTCTCTGTCAGTTTGGGGGGCGTCAAAAGAGCTTTGGGATAGCCGCCCCGAAGGGAGCGGCTGGAGCTGGAAGGA                                     >  SRR4302537.6418425/1‑94 (MQ=60)

                                                         GCCATGTTTGGATTGATGTTCTCTGTCAGTTTGGGGGGCGTCAAAAGAGCTTTGGGATAGCCGCCCCGAAGGGAGCGGCTGGAGCTGGAAGGAT                                    >  SRR4302537.5860630/1‑94 (MQ=60)

                                                           CATGTTTGGATTGATGTTCTCTGTCAGTTTGGGGGGCGTCAAAAGAGCTTTGGGATAGCCGCCCCGAAGGGAGCGGCTGGAGCTGGAAGGATTT                                  >  SRR4302537.2936398/1‑94 (MQ=60)

                                                            ATGTTTGGATTGATGTTCTCTGTCAGTTTGGGGGGCGTCAAAAGAGCTTTGGGATAGCCGCCCCGAAGGGAGCGGCTGGAGCTGGAAGGATTTG                                 <  SRR4302537.1753105/94‑1 (MQ=60)

                                                            ATGTTTGGATTGATGTTCTCTGTCAGTTTGGGGGGCGTCAAAAGAGCTTTGGGATAGCCGCCCCGAAGGGAGCGGCTGGAGCTGGAAGGATTTG                                 >  SRR4302537.3056490/1‑94 (MQ=60)

                                                             TGTTTGGATTGATGTTCTCTGTCAGTTTGGGGGGCGTCAAAAGAGCTTTGGGATAGCCGCCCCGAAGGGAGCGGCTGGAGCTGGAAGGATTTGG                                >  SRR4302537.2648196/1‑94 (MQ=60)

                                                              GTTTGGATTGATGTTCTCTGTCAGTTTGGGGGGCGTCAAAAGAGCTTTGGGATAGCCGCCCCGAAGGGAGCGGCTGGAGCTGGAAGGATTTGGC                               >  SRR4302537.1539689/1‑94 (MQ=60)

                                                                 TGGATTGATGTTCTCTGTCAGTTTGGGGGGCGTCAAAAGAGCTTTGGGATAGCCGCCCCGAAGGGAGCGGCTGGAGCTGGAAGGATTTGGTCTG                            >  SRR4302537.2025575/1‑94 (MQ=60)

                                                                 TGGATTGATGTTCTCTGTCAGTTTGGGGGGCGTCAAAAGAGCTTTGGGATAGCCGCCCCGAAGGGAGCGGCTGGAGCTGGAAGGATTTGGCCTG                            <  SRR4302537.892719/94‑1 (MQ=60)

                                                                 TGGATTGATGTTCTCTGTCAGTTTGGGGGGCGTCAAAAGAGCTTTGGGATAGCCGCCCCGAAGGGAGCGGCTGGAGCTGGAAGGATTTGGCCTG                            >  SRR4302537.2025574/1‑94 (MQ=60)

                                                                 TGGATTGATGTTCTCTGTCAGTTTGGGGGGCGTCAAAAGAGCTTTGGGATAGCCGCCCCGAAGGGAGCGGCTGGAGCTGGAAGGATTTGGCCTG                            >  SRR4302537.1805966/1‑94 (MQ=60)

                                                                  GGATTGATGTTCTCTGTCAGTTTGGGGGGCGTCAAAAGAGCTTTGGGATAGCCGCCCCGAAGGGAGCGGCTGGAGCTGGAAGGATTTGGCCTGG                           <  SRR4302537.277566/94‑1 (MQ=60)

                                                                    ATTGATGTTCTCTGTCAGTTTGGGGGGCGTCAAAAGAGCTTTGGGATAGCCGCCCCGAAGGGAGCGGCTGGAGCTGGAAGGATTTGGCCTGGGA                         >  SRR4302537.4320830/1‑94 (MQ=60)

                                                                      TGATGTTCTCTGTCAGTTTGGGGGGCGTCAAAAGAGCTTTGGGATAGCCGCCCCGAAGGGAGCGGCTGGAGCTGGAAGGATTTGGCCTGGGATG                       >  SRR4302537.416217/1‑94 (MQ=60)

                                                                      TGATGTTCTCTGTCAGTTTGGGGGGCGTCAAAAGAGCTTTGGGATAGCCGCCCCGAAGGGAGCGGCTGGAGCTGGAAGGATTTGGCCTGGGATG                       <  SRR4302537.2560257/94‑1 (MQ=60)

                                                                      TGATGTTCTCTGTCAGTTTGGGGGGCGTCAAAAGAGCTTTGGGATAGCCGCCCCGAAGGGAGCGGCTGGAGCTGGAAGGATTTGGCCTGGGATG                       <  SRR4302537.1263967/94‑1 (MQ=60)

                                                                       GATGTTCTCTGTCAGTTTGGGGGGCGTCAAAAGAGCTTTGGGATAGCCGCCCCGAAGGGAGCGGCTGGAGCTGGAAGGATTTGGCCTGGGATGT                      >  SRR4302537.1864603/1‑94 (MQ=60)

                                                                       GATGTTCTCTGTCAGTTTGGGGGGCGTCAAAAGAGCTTTGGGATAGCCGCCCCGAAGGGAGCGGCTGGAGCTGGAAGGATTTGGCCTGGGATGT                      <  SRR4302537.3864808/94‑1 (MQ=60)

                                                                       GATGTTCTCTGTCAGTTTGGGGGGCGTCAAAAGAGCTTTGGGATAGCCGCCCCGAAGGGAGCGGCTGGAGCTGGAAGGATTTGGCCTGGGATGT                      <  SRR4302537.2937342/94‑1 (MQ=60)

                                                                        ATGTTCTCTGTCAGTTTGGGGGGCGTCAAAAGAGCTTTGGGATAGCCGCCCCGAAGGGAGCGGCTGGAGCTGGAAGGATTTGGCCTGGGATGTC                     <  SRR4302537.4680376/94‑1 (MQ=60)

                                                                        ATGTTCTCTGTCAGTTTGGGGGGCGTCAAAAGAGCTTTGGGATAGCCGCCCCGAAGGGAGCGGCTGGAGCTGGAAGGATTTGGCCTGGGATGTC                     <  SRR4302537.2372525/94‑1 (MQ=60)

                                                                        ATGTTCTCTGTCAGTTTGGGGGGCGTCAAAAGAGCTTTGGGATAGCCGCCCCGAAGGGAGCGGCTGGAGCTGGAAGGATTTGGCCTGGGATGTC                     <  SRR4302537.4896811/94‑1 (MQ=60)

                                                                        ATGTTCTCTGTCAGTTTGGGGGGCGTCAAAAGAGCTTTGGGATAGCCGCCCCGAAGGGAGCGGCTGGAGCTGGAAGGATTTGGCCTGGGATGTC                     >  SRR4302537.4850460/1‑94 (MQ=60)

                                                                        ATGTTCTCTGTCAGTTTGGGGGGCGTCAAAAGAGCTTTGGGATAGCCGCCCCGAAGGGAGCGGCTGGAGCTGGAAGGATTTGGCCTGGGATGTC                     >  SRR4302537.2874220/1‑94 (MQ=60)

                                                                           TTCTCTGTCAGTTTGGGGGGCGTCAAAAGAGCTTTGGGATAGCCGCCCCGAAGGGAGCGGCTGGAGCTGGAAGGATTTGGCCTGGGATGTCGCC                  >  SRR4302537.4101767/1‑94 (MQ=60)

                                                                           TTCTCTGTCAGTTTGGGGGGCGTCAAAAGAGCTTTGGGATAGCCGCCCCGAAGGGAGCGGCTGGAGCTGGAAGGATTTGGCCTGGGATGTCGCC                  >  SRR4302537.2529927/1‑94 (MQ=60)

                                                                           TTCTCTGTCAGTTTGGGGGGCGTCAAAAGAGCTTTGGGATAGCCGCCCCGAAGGGAGCGGCTGGAGCTGGAAGGATTTGGCCTGGGATGTCGCC                  <  SRR4302537.5504049/94‑1 (MQ=60)

                                                                            TCTCTGTCAGTTTGGGGGGCGTCAAAAGAGCTTTGGGATAGCCGCCCCGAAGGGAGCGGCTGGAGCTGGAAGGATTTGGCCTGGGATGTCGCCG                 >  SRR4302537.2079302/1‑94 (MQ=60)

                                                                              TCTGTCAGTTTGGGGGGCGTCAAAAGAGCTTTGGGATAGCCGCCCCGAAGGGAGCGGCTGGAGCTGGAAGGATTTGGCCTGGGATGTCGCCGGT               <  SRR4302537.2973594/94‑1 (MQ=60)

                                                                              TCTGTCAGTTTGGGGGGCGTCAAAAGAGCTTTGGGATAGCCGCCCCGAAGGGAGCGGCTGGAGCTGGAAGGATTTGGCCTGGGATGTCGCCGGT               <  SRR4302537.4290007/94‑1 (MQ=60)

                                                                               CTGTCAGTTTGGGGGGCGTCAAAAGAGCTTTGGGATAGCCGCCCCGAAGGGAGCGGCTGGAGCTGGAAGGATTTGGCCTGGGATGTCGGCGGTG              >  SRR4302537.3392893/1‑94 (MQ=60)

                                                                               CTGTCAGTTTGGGGGGCGTCAAAAGAGCTTTGGGATAGCCGCCCCGAAGGGAGCGGCTGGAGCTGGAAGGATTTGGCCTGGGATGTCGCCGGTG              <  SRR4302537.3835787/94‑1 (MQ=60)

                                                                                TGTCAGTTTGGGGGGCGTCAAAAGAGCTTTGGGATAGCCGCCCCGAAGGGAGCGGCTGGAGCTGGAAGGATTTGGCCTGGGATGTCGCCGGTGG             >  SRR4302537.200281/1‑94 (MQ=60)

                                                                                TGTCAGTTTGGGGGGCGTCAAAAGAGCTTTGGGATAGCCGCCCCGAAGGGAGCGGCTGGAGCTGGAAGGATTTGGCCTGGGATGTCGCCGGTGC             >  SRR4302537.1333657/1‑94 (MQ=60)

                                                                                 GTCAGTTTGGGGGGCGTCAAAAGAGCTTTGGGATAGCCGCCCCGAAGGGAGCGGCTGGAGCTGGAAGGATTTGGCCTGGGATGTCGCCGGTGCA            >  SRR4302537.6513383/1‑94 (MQ=60)

                                                                                   agtTTT‑GGGGGCGTCAAAAGAGCTTTGGGATAGCCGCCCCGAAGGGAGCGGCTGGAGCTGGAAGGATTTGGCCTGGGATGTCGCCGGTGCAAGC         >  SRR4302537.402968/4‑94 (MQ=60)

                                                                                   CAGTTTGGGGGGCGTCAAAAGAGCTTTGGGATAGCCGCCCCGAAGGGAGCGGCTGGAGCTGGAAGGATTTGGCCTGGGATGTCGCCGGTGCAAG          <  SRR4302537.3154773/94‑1 (MQ=60)

                                                                                    AGTTTGGGGGGCGTCAAAAGAGCTTTGGGATAGCCGCCCCGAAGGGAGCGGCTGGAGCTGGAAGGATTTGGCCTGGGATGTCGCCGGTGaaaga         >  SRR4302537.4360407/1‑89 (MQ=60)

                                                                                    AGTTTGGGGGGCGTCAAAAGAGCTTTGGGATAGCCGCCCCGAAGGGAGCGGCTGGAGCTGGAAGGATTTGGCCTGGGATGTCGCCGGTGCAAGC         >  SRR4302537.112957/1‑94 (MQ=60)

                                                                                    AGTTTGGGGGGCGTCAAAAGAGCTTTGGGATAGCCGCCCCGAAGGGAGCGGCTGGAGCTGGAAGGATTTGGCCTGGGATGTCGCCGGTGCAAGC         >  SRR4302537.322959/1‑94 (MQ=60)

                                                                                    AGTTTGGGGGGCGTCAAAAGAGCTTTGGGATAGCCGCCCCGAAGGGAGCGGCTGGAGCTGGAAGGATTTGGCCTGGGATGTCGCCGGTGCAAGC         >  SRR4302537.3670019/1‑94 (MQ=60)

                                                                                    AGTTTGGGGGGCGTCAAAAGAGCTTTGGGATAGCCGCCCCGAAGGGAGCGGCTGGAGCTGGAAGGATTTGGCCTGGGATGTCGCCGGTGCAAGC         >  SRR4302537.2420622/1‑94 (MQ=60)

                                                                                     GTTTGGGGGGCGTCAAAAGAGCTTTGGGATAGCCGCCCTGAAGGGAGCGGCTGGAGCTGGAAGGATTTGGCCTGGGATGTCGCCGGTGCAAGCA        <  SRR4302537.2541623/94‑1 (MQ=60)

                                                                                     GTTTGGGGGGCGTCAAAAGAGCTTTGGGATAGCCGCCCCGAAGGGAGCGGCTGGAGCTGGAAGGATTTGGCCTGGGATGTCGCCGGTGCAAGCA        <  SRR4302537.2140471/94‑1 (MQ=60)

                                                                                      TTTGGGGGGCGTCAAAAGAGCTTTGGGATAGCCGCCCCGAAGGGAGCGGCTGGAGCTGGAAGGATTTGGCCTGGGATGTCGCCGGTGCGAGCAC       >  SRR4302537.2301516/1‑94 (MQ=60)

                                                                                        G‑GGGGGCGTCAAAAGAGCTTTGGGATAGCCGCCCCGAAGGGAGCGGCTGGAGCTGGAAGGATTTGGCCTGGGATGTCGCCGGTGCAAGCACCGG    <  SRR4302537.1633303/94‑1 (MQ=60)

                                                                                           GGGGCGTCAAAAGAGCTTTGGGATAGCCGCCCCGAAGGGAGCGGCTGGAGCTGGAAGGATTTGGCCTGGGATGTCGCCGGTGCAAGCACCGGCT  <  SRR4302537.2965556/94‑1 (MQ=60)

CTTTCCGCCGCCGGAAATGAATATTCACAGCATCAGGGGATGAGCCGGGATCGCAGTGCCATGTTTGGATTGATGTTCTCTGTCAGTTT‑GGGGGCGTCAAAAGAGCTTTGGGATAGCCGCCCCGAAGGGAGCGGCTGGAGCTGGAAGGATTTGGCCTGGGATGTCGCCGGTGCAAGCACCGGCT  >  NC_000913/2722259‑2722442

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 0 ≤` `ATCG/ATCG` `< 16 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`