Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A1 F1 I2 R1	882	254.4	12957588	98.0%	12698436	93.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,738,867	T→C	T102A (ACT→GCT)	aroF ←	3‑deoxy‑D‑arabinoheptulosonate‑7‑phosphate synthase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,738,867	0	T	C	95.9%	786.0 / 15.4	246	T102A (ACT→GCT)	aroF	3‑deoxy‑D‑arabinoheptulosonate‑7‑phosphate synthase
Reads supporting (aligned to +/- strand): ref base T (4/6); new base C (128/108); total (132/114)
Fisher's exact test for biased strand distribution p-value = 5.21e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.95e-01

Only 100 of 253 total aligned reads displayed.

GCAGCAATTTACGCGCGATCTGCAGCCCGGCTTCTACATCAAAAGAGCCATCCATATGGGGATCGTTAATTAACCCTTTCCAGCCGACAGTGGTACGGGGTTTTTCAAAATAGACGCGCATTACCAGATAGAGGCTATCGCTGACCTCTGCGGCAAGGGCTTTAAATCGACGAGCATATT  >  NC_000913/2738777‑2738956

gcagcaATTTACGCGCGATCTGCAGCCCGGCTTCTACATCAAAAGAGCCATCCATATGGGGATCGTTAATTAACCCTTTCCAGCCGACAGCgg                                                                                         <  2:3945/93‑1 (MQ=255)

  agcaATTTACGCGCGATCTGCAGCCCGGCTTCTACATCAAAAGAGCCATCCATATGGGGATCGTTAATTAACCCTTTCCAGCCGACAGCGGTa                                                                                       <  2:2482339/93‑1 (MQ=255)

  agcaATTTACGCGCGATCTGCAGCCCGGCTTCTACATCAAAAGAGCCATCCATATGGGGATCGTTAATTAACCCTTTCCAGCCGACAGCGGTa                                                                                       <  2:4077818/93‑1 (MQ=255)

  agcaATTTACGCGCGATCTGCAGCCCGGCTTCTACATCAAAAGAGCCATCCATATGGGGATCGTTAATTAACCCTTTCCAGCCGACAGCGGTa                                                                                       >  2:5194237/1‑93 (MQ=255)

   gcaATTTACGCGCGATCTGCAGCCCGGCTTTTACATCAAAAGAGCCATCCATATGGGGATCGTTAATTAACCCTTTCCAGCCGACAGCGGTAc                                                                                      <  1:4537350/93‑1 (MQ=255)

   gcaATTTACGCGCGATCTGCAGCCCGGCTTCTACATCAAAAGAGCCATCCATATGGGGATCGTTAATTAACCCTTTCCAGCCGACAGCGGTAc                                                                                      >  1:4991818/1‑93 (MQ=255)

   gcaATTTACGCGCGATCTGCAGCCCGGCTTCTACATCAAAAGAGCCATCCATATGGGGATCGTTAATTAACCCTTTCCAGCCGACAGCGGTAc                                                                                      <  2:430727/93‑1 (MQ=255)

    caATTTACGCGCGATCTGCAGCCCGGCTTCTACATCAAAAGAGCCATCCATATGGGGATCGTTAATTAACCCTTTCCAGCCGACAGCGGTACg                                                                                     >  1:2296212/1‑93 (MQ=255)

      aTTTACGCGCGATCTGCAGCCCGGCTTCTACATCAAAAGAGCCATCCATATGGGGATCGTTAATTAACCCTTTCCAGCCGACAGCGGTACggg                                                                                   >  1:4916506/1‑93 (MQ=255)

       tttACGCGCGATCTGCAGCCCGGCTTCTACATCAAAAGAGCCATCCATATGGGGATCGTTAATTAACCCTTTCCAGCCGACAGCGGTACgggg                                                                                  <  2:189568/93‑1 (MQ=255)

       tttACGCGCGATCTGCAGCCCGGCTTCTACATCAAAAGAGCCATCCATATGGGGATCGTTAATTAACCCTTTCCAGCCGACAGCGGTACgggg                                                                                  >  1:2516658/1‑93 (MQ=255)

        ttACGCGCGATCTGCAGCCCGGCTTCTACATCAAAAGAGCCATCCATATGGGGATCGTTAATTAACCCTTTCCAGCCGACAGCGGTACGGGGt                                                                                 >  2:4852725/1‑93 (MQ=255)

          aCGCGCGATCTGCAGCCCGGCTTCTACATCAAAAGAGCCATCCATATGGGGATCGTTAATTAACCCTTTCCAGCCGACAGCGGTACGGGGttt                                                                               <  2:2211476/93‑1 (MQ=255)

           cgcgcgATCTGCAGCCCGGCTTCTACATCAAAAGAGCCATCCATATGGGGATCGTTAATTAACCCTTTCCAGCCGACAGCGGTACGGGGtttt                                                                              <  2:1682142/93‑1 (MQ=255)

           cgcgcgATCTGCAGCCCGGCTTCTACATCAAAAGAGCCATCCATATGGGGATCGTTAATTAACCCTTTCCAGCCGACAGCGGTACGGGGtttt                                                                              <  2:6343856/93‑1 (MQ=255)

            gcgcgATCTGTAGCCCGGCTTCTACATCAAAAGAGCCATCCATATGGGGATCGTTAATTAACCCTTTCCAGCCGACAGCGGTACGGGGttttt                                                                             >  2:3097387/1‑93 (MQ=255)

            gcgcgATCTGCAGCCCGGCTTCTACATCAAAAGAGCCATCCATATGGGGATCGTTAATTAACCCTTTCCAGCCGACAGCGGTACGGGGttttt                                                                             <  1:75656/93‑1 (MQ=255)

            gcgcgATCTGCAGCCCGGCTTCTACATCAAAAGAGCCATCCATATGGGGATCGTTAATTAACCCTTTCCAGCCGACAGCGGTACGGGGttttt                                                                             <  2:5466279/93‑1 (MQ=255)

             cgcgATCTGCAGCCCGGCTTCTACATCAAAAGAGCCATCCATATGGGGATCGTTAATTAACCCTTTCCAGCCGACAGCGGTACGGGGTTTTTc                                                                            >  1:1939158/1‑93 (MQ=255)

              gcgATCTGCAGCCCGGCTTCTACATCAAAAGAGCCATCCATATGGGGATCGTTAATTAACCCTTTCCAGCCGACAGCGGTACGGGGTTTTTCa                                                                           >  1:2102079/1‑93 (MQ=255)

              gcgATCTGCAGCCCGGCTTCTACATCAAAAGAGCCATCCATATGGGGATCGTTAATTAACCCTTTCCAGCCGACAGCGGTACGGGGTTTTTCa                                                                           >  2:4149738/1‑93 (MQ=255)

               cgATCTGCAGCCCGGCTTCTACATCAAAAGAGCCATCCATATGGGGATAGTTAATTAACCCTTTCCAGCCGACAGCGGTACGGGGTTTTTCaa                                                                          <  2:3415264/93‑1 (MQ=255)

                gATCTGCAGCCCGGCTTCTACATCAAAAGAGCCATCCATATGGGGATCGTTAATTAACCCTTTCCAGCCGACAGCGGTACGGGGTTTTTCaaa                                                                         >  2:210233/1‑93 (MQ=255)

                gATCTGCAGCCCGGCTTCTACATCAAAAGAGCCATCCATATGGGGATCGTTAATTAACCCTTTCCAGCCGACAGCGGTACGGGGTTTTTCaaa                                                                         >  1:3840131/1‑93 (MQ=255)

                 aTCTGCAGCCCGGCTTCTACATCAAAAGAGCCATCCATATGGGGATCGTTCATTAACCCTTTCCAGCCGACAGCGGTACGGGGTTTTTCaaaa                                                                        >  1:5965871/1‑93 (MQ=255)

                 aTCTGCAGCCCGGCTTCTACATCAAAAGAGCCATCCATATGGGGATCGTTAATTAACCCTTTCCAGCCGACAGCGGTACGGGGTTTTTCaaaa                                                                        >  1:242386/1‑93 (MQ=255)

                  tCTGCAGCCCGGCTTCTACATCAAAAGAGCCATCCATATGGGGATCGTTAATTAACCCTTTCCAGCCGACAGCGGTACGGGGTTTTTCAAAAt                                                                       <  2:1784659/93‑1 (MQ=255)

                  tCTGCAGCCCGGCTTCTACATCAAAAGAGCCATCCATATGGGGATCGTTAATTAACCCTTTCCAGCCGACAGCGGTACGGGGTTTTTCAAAAt                                                                       <  1:1712451/93‑1 (MQ=255)

                     gCAGCCCGGCTTCTACATCAAAAGAGCCATCCATATGGGGATCGTTAATTAACCCTTTCCAGCCGACAGCGGTACGGGGTTTTTCAAAATAGa                                                                    <  1:1605597/93‑1 (MQ=255)

                     gCAGCCCGGCTTCTACATCAAAAGAGCCATCCATATGGGGATCGTTAATTAACCCTTTCCAGCCGACAGCGGTACGGGGTTTTTCAAAATAGa                                                                    <  2:3490530/93‑1 (MQ=255)

                       aGCCCGGCTTCTACATCAAAAGAGCCATCCATATGGGGATCGTTAATTAACCCTTTCCAGCCGACAGCGGTACGGGGTTTTTCAAAATAGAcg                                                                  >  2:1217111/1‑93 (MQ=255)

                        gCCCGGCTTCTACATCAAAAGAGCCATCCATATGGGGATCGTTAATTAACCCTTTCCAGCCGACAGCGGTACGGGGTTTTTCAAAATAGAcgc                                                                 <  1:6096666/93‑1 (MQ=255)

                        gCCCGGCTGCTACATCAAAAGAGCCATCCATATGGGGATCGTTAATTAACCCTTTCCAGCCGACAGTGGTACGGGGTTTTTCAAAATAGAcgc                                                                 >  2:6295455/1‑93 (MQ=255)

                          ccGGCTTCTACATCAAAAGAGCCATCCATATGGGGATCGTTAATTAACCCTTTCCAGCCGACAGCGGTACGGGGTTTTTCAAAATAGACgcgc                                                               >  2:354776/1‑93 (MQ=255)

                            ggCTTCTACATCAAAAGAACCATCCATATGGGGATCGTTAATTAACCCTTTCCAGCCGACAGCGGTACGGGGTTTTTCAAAATAGACGCGCAt                                                             >  1:2155173/1‑93 (MQ=255)

                             gCTTCTACATCAAAAGAGCCATCCATATGGGGATCGTTAATTAACCCTTTCCAGCCGACAGCGGTACGGGGTTTTTCAAAATAGACGCGCAtt                                                            >  1:3438490/1‑93 (MQ=255)

                             gCTTCTACATCAAAAGAGCCATCCATATGGGGATCGTTAATTAACCCTTTCCAGCCGACAGCGGTACGGGGGTTTTCAAAATAGACGCGCAtt                                                            >  2:4567496/1‑93 (MQ=255)

                              cTTCTACATCAAAAGAGCCATCCATATGGGGATCGTTAATTAACCCTTTCCAGCCGACAGCGGTACGGGGTTTTTCAAAATAGACGCGCATTa                                                           >  2:724689/1‑93 (MQ=255)

                               ttCTACATCAAAAGAGCCATCCATATGGGGATCGTTAATTAACCCTTTCCAGCCGACAGCGGTACGGGGTTTTTCAAAATAGACGCGCATTAc                                                          <  1:4518910/93‑1 (MQ=255)

                                 cTACATCAAAAGAGCCATCCATATGGGGATCGTTAATTAACCCTTTCCAGCCGACAGCGGTACGGGGTTTTTCAAAATAGACGCGCATTACCa                                                        >  2:5090212/1‑93 (MQ=255)

                                 cTACATCAAAAGAGCCATCCATATGGGGATCGTTAATTAACCCTTTCCAGCCGACAGCGGTACGGGGGTTTTCAAAATAGACGCGCATTAACa                                                        >  1:2316494/1‑93 (MQ=255)

                                  tACATCAAAAGAGCCATCCATATGGGGATCGTTAATTAACCCTTTCCAGCCGACAGCGGTACGGGGTTTTTCAAAATAGACGCGCATTACGAg                                                       <  2:3619389/93‑1 (MQ=255)

                                       cAAAAGAGCCATCCATATGGTGATCGTTAATTAACCCTTTCCAGCCGACAGCGGTACGGGGTTTTTCAAAATAGACGCGCATTACCAGATaga                                                  <  2:5310814/93‑1 (MQ=255)

                                       cAAAAGAGCCATCCATACGGGGATCGTTAATTAACCCTTTCCAGCCGACAGCGGTACGGGGTTTTTCAAAATAGACGCGCATTACCAGATaga                                                  <  2:1641563/93‑1 (MQ=255)

                                        aaaaGAGCCATCCATATGGGGATCGTTAATTAACCCTTTCCAGCCGACAGCGGTACGGGGTTTTTCAAAATAGACGCGCATTACCAGATagag                                                 >  2:6084681/1‑93 (MQ=255)

                                         aaaGAGCCATCCATATGGGGATCGTTAATTAACCCTTTCCAGCCGACAGCGGTACGGGGTTTTTCAAAATAGACGCGCATTACCAGATAGAgg                                                <  1:4172476/93‑1 (MQ=255)

                                         aaaGAGCCATCCATATGGGGATCGTTAATTAACCCTTTCCAGCCGACAGCGGTACGGGGGTTTTCAAAATAGACGCGCATTACCAGATagaag                                                >  2:5567975/1‑91 (MQ=255)

                                          aaGAGCCATCCATATGGGGATCGTTAATTACCCCTTTCCAGCCGACAGCGGTACGGGGTTTTTCAAAATAGACGCGCATTACCAGATAGAGGc                                               <  2:376481/93‑1 (MQ=255)

                                           agagCCATCCATATGGGGATCGTTAATTAACCCTTTCCAGCCGACAGCGGTACGGGGTTTTTCAAAATAGACGCGCATTACCAGATAGAGGCt                                              >  1:1044430/1‑93 (MQ=255)

                                           agagCCATCCATATGGGGATCGTTAATTAACCCTTTCCAGCCGACAGCGGTACGGGGTTTTTCAAAATAGACGCGCATTACCAGATAGAGGCt                                              >  2:1473202/1‑93 (MQ=255)

                                           agagCCATCCATATGGGGATCGTTAATTAACCCTTTCCAGCCGACAGCGGTACGGGGTTTTTCAAAATAGACGCGCATTACCAGATAGAGGCt                                              >  2:3833630/1‑93 (MQ=255)

                                            gagCCATCCATATGGGGATCGTTAATTAACCCTTTCCAGCCGACAGCGGTACGGGGTTTTTCAAAATAGACGCGCATTACCAGATAGAGGCTa                                             <  2:4659143/93‑1 (MQ=255)

                                              gCCATCCATATGGGGATCGTTAATTAACCCTTTCCAGCCGACAGCGGTACGGGGTTTTTCAAAATAGACGCGCATTACCAGATAGAGGCTATc                                           <  1:1092098/93‑1 (MQ=255)

                                              gCCATCCATATGGGGATCGTTAATTAACCCTTTCCAGCCGACAGCGGTACGGGGTTTTTCAAAATAGACGCGCATTACCAGATAGAGGCTATc                                           <  1:3528079/93‑1 (MQ=255)

                                                catccatATGGGGATCGTTAATTAACCCTTTCCAGCCGACAGCGGTACGGGGTTTTTCAAAATAGACGCGCATTACCAGATAGAGGCTATCGc                                         >  2:5416623/1‑93 (MQ=255)

                                                catccatATGGGGATCGTTAATTAACCCTTTCCAGCCGACAGCGGTACGGGGTTTTTCAAAATAGACGCGCATTACCAGATAGAGGCTATCGc                                         <  1:4663944/93‑1 (MQ=255)

                                                catccatATGGGGATCGTTAATTAACCCTTTCCAGCCGACAGCGGTACGGGGTTTTTCAAAATAGACGCGCATTACCAGATAGAGGCTATCGc                                         >  2:3257204/1‑93 (MQ=255)

                                                  tccatATGGGGATCGTTAATTAACCCTTTCCAGCCGACAGCGGTACGGGGTTTTTCAAAATAGACGCGCATTACCAGATAGAGGCTATCGCTg                                       >  1:37830/1‑93 (MQ=255)

                                                    catATGGGTATCGTTAATTAACCCTTTCCAGCCGACAGCGGTACGGGGTTTTTCAAAATAGACGCGCATTACCAGATAGAGGCTATCGCTGAc                                     <  1:5646278/93‑1 (MQ=255)

                                                    catATGGGGATCGTTAATTAACCCTTTCCAGCCGACAGCGGTACGGGGTTTTTCAAAATAGACGCGCATTACCAGATAGAGGCTATCGCTGAc                                     >  1:3959627/1‑93 (MQ=255)

                                                     atatGGGGCTCGTTAATTAACCCTTTCCAGCCGACAGCGGTACGGGGTTTTTCAAAATAGACGCGCATTACCATATATAGGATATCGCTGAct                                    >  1:5116170/1‑92 (MQ=25)

                                                     atatGGGGATCGTTAATTAACCCTTTCCAGCCGACAGCGGTACGGGGTTTTTCAAAATAGACGCGCATTACCAGATAGAGGCTATCGCTGAcc                                    >  1:4118068/1‑93 (MQ=255)

                                                     atatGGGGATCGTTAATTAACCCTTTCCAGCCGACAGCGGTACGGGGTTTTTCAAAATAGACGCGCATTACCAGATAGAGGCTATCGCTGAcc                                    >  1:5393565/1‑93 (MQ=255)

                                                      tatGGGGATCGTTAATTAACCCTTTCCAGCCGACAGCGGTACGGGGTTTTTCAAAATAGACGCGCATTACCAGATAGAGGCTATCGCTGACct                                   >  2:266705/1‑93 (MQ=255)

                                                      tatGGGGATCGTTAATTAACCCTTTCCAGCCGACAGCGGTACGGGGTTTTTCAAAATAGACGCGCATTACCAGATAGAGGCTATCGCTGACct                                   <  2:3020635/93‑1 (MQ=255)

                                                        tGGGGATCGTTAATTAACCCTTTCCAGCCTACAGCGGTACGGGGTTTTTCAAAATAGACGCGCATTACCAGATAGAGGCTATCGCTGACctct                                 >  1:5522866/1‑93 (MQ=255)

                                                        tGGGGATCGTTAATTAACCCTTTCCAGCCGACAGCGGTACGGGGTTTTTCAAAATAGACGCGCATTACCAGATAGAGGCTATCGCTGACctct                                 <  1:2003326/93‑1 (MQ=255)

                                                        tGGGGATCGTTAATTAACCCTTTCCAGCCGACAGCGGTACGGGGTTTTTCAAAATAGACGCGCATTACCAGATAGAGGCTATCGCTGACctct                                 >  2:5108707/1‑93 (MQ=255)

                                                          gggATCGTTAATTAACCCTTTCCAGCCGACAGCGGTACGGGGTTTTTCAAAATAGACGCGCATTACCAGATAGAGGCTATCGCTGACCTCTGc                               >  2:278534/1‑93 (MQ=255)

                                                          gggATCGTTAATTAACCCTTTCCAGCCGACAGCGGTACGGGGTTTTTCAAAATAGACGCGCATTACCAGATAGAGGCTATCGCTGACCTCTGc                               >  1:298206/1‑93 (MQ=255)

                                                          gggATCGTTAATTAACCCTTTCCAGCCGACAGCGGTACGGGGTTTTTCAAAATAGACGCGCATTACCAGATAGAGGCTATCGCTGACCTCTGc                               <  1:6461351/93‑1 (MQ=255)

                                                             aTCGTTAATTAACCCTTTCCAGCCGACAGCGGTACGGGGTTTTTCAAAATAGACGCGCATTACCAGATAGAGGCTATCGCTGACCTCTGCGGc                            >  2:299541/1‑93 (MQ=255)

                                                             aTCGTTAATTAACCCTTTCCAGCCGACAGCGGTACGGGGTTTTTCAAAATAGACGCGCATTACCAGATAGAGGCTATCGCTGACCTCTGCGGc                            >  1:3079078/1‑93 (MQ=255)

                                                             aTCGTTAATTAACCCTTTCCAGCCGACAGCGGTACGGGGTTTTTCAAAATAGACGCGCATTACCAGATAGAGGCTATCGCTGACCTCTGCGGc                            >  2:5829312/1‑93 (MQ=255)

                                                             aTCGTTAATTAACCCTTTCCAGCCGACAGCGGTACGGGGTTTTTCAAAATAGACGCGCATTACCAGATAGAGGCTATCGCTGACCTCTGCGGc                            <  1:3870831/93‑1 (MQ=255)

                                                              tCGTTAATTAACCCTTTCCAGCCGACAGCGGTACGGGGTTTTTCAAAATAGACGCGCATTACCAGATAGAGGCTATCGCTGACCTCTGCGGCa                           >  1:1484807/1‑93 (MQ=255)

                                                              tCGTTAATTAACCCTTTCCAGCCGACAGCGGTACGGGGTTTTTCAAAATAGACGCGCATTACCAGATAGAGGCTATCGCTGACCTCTGCGGCa                           >  1:1942730/1‑93 (MQ=255)

                                                                gTTAATTAACCCTTTCCAGCCGACAGCGGTACGGGGTTTTTCAAAATAGACGCGCATTACCAGATAGAGGCTATCGCTGACCTCTGCGGCAAg                         >  2:559471/1‑93 (MQ=255)

                                                                 ttaattaaCCCTTTCCAGCCGACAGCGGTACGGGGTTTTTCAAAATAGACGCGCGTTACCAGATAGAGGCTATCGCTGACCTCTGCGGCAAgg                        <  1:4065442/93‑1 (MQ=255)

                                                                     ttaaCCCTTTCCAGCCGACAGCGGTACGGGGTTTTTCAAAATAGACGCGCATTACCAGATAGAGGCTATCGCTGACCTCTGCGGCAAGGGCtt                    <  1:6181646/93‑1 (MQ=255)

                                                                     ttaaCCCTTTCCAGCCGACAGCGGTACGGGGTTTTTCAAAATAGACGCGCATTACCAGATAGAGGCTATCGCTGACCTCTGCGGCAAGGGCtt                    <  1:887434/93‑1 (MQ=255)

                                                                      taaCCCTTTCCAGCCGACAGCGGTACGGGGTTTTTCAAAATAGACGCGCATTACCAGATAGAGGCTATCGCTGACCTCTGCGGCAAGGGCttt                   <  1:5746123/93‑1 (MQ=255)

                                                                       aaCCCTTTCCAGCCGACAGCGGTACGGGGTTTTTCAAAATAGACGCGCATTACCAGATAGAGGCTATCGCTGACCTCTGCGGCAAGGGCTTTa                  <  1:1874927/93‑1 (MQ=255)

                                                                        aCCCTTTCCAGCCGACAGCGGTACGGGGCTTTTCAAAATAGACGCGCATTACCAGATAGAGGCTATCGCTGACCTCTGCGGCAAGGGCTTTaa                 >  1:63953/1‑93 (MQ=255)

                                                                         cccTTTCCAGCCGACAGCGGTACGGGGTTTTTCATAATAGACGCGCATTACCAGATAGAGGCTATCGCTGACCTCTGCGGCAAGGGCTTTaaa                >  1:4370293/1‑93 (MQ=255)

                                                                          ccTTTCCAGCCGACAGTGGTACGGGGTTTTTCAAAATAGACGCGCATTACCAGATAGAGGCTATCGCTGACCTCTGCGGCAAGGGCTTTAAAt               <  2:2334031/93‑1 (MQ=255)

                                                                          ccTTTCCAGCCGACAGCGGTACGGGGTTTTTCAAAATAGACGCGCATTACCAGATAGAGGCTATCGCTGACCTCTGCGGCAAGGGCTTTAAAt               <  1:6303859/93‑1 (MQ=255)

                                                                          ccTTTCCAGCCGACAGCGGTACGGGGTTTTTCAAAATAGACGCGCATTACCAGATAGAGGCTATCGCTGACCTCTGCGGCAAGGGCTTTAAAt               <  1:1424323/93‑1 (MQ=255)

                                                                           cTTTCCAGCCGACAGCGGTACGGGGTTTTTCAAAATAGACGCGCATTACCAGATAGAGGCTATCGCTGACCTCTGCGGCAAGGGCTTTAAATc              >  1:480065/1‑93 (MQ=255)

                                                                              tCCAGCCGACAGCGGTACGGGGTTTTTCAAAATAGACGCGCATTACCAGATAGAGGCTATCGCTGACCTCTGCGGCAAGGGCTTTAAATcgac           <  2:4508657/93‑1 (MQ=255)

                                                                              tCCAGCCGACAGCGGTACGGGGTTTTTCAAAATAGACGCGCATTACCAGATAGAGGCTATCGCTGACCTCTGCGGCAAGGGCTTTAAATcgac           <  1:403503/93‑1 (MQ=255)

                                                                               ccAGCCGACAGCGGTACGGTGTTTTTCAAAATAGACGCGCATTACCAGATAGAGGCTATCGCTGACCTCTGCGGCAAGGGCTTGAAATcgacg          <  2:947675/93‑1 (MQ=255)

                                                                                    cGACAGTGGTACGGGGTTTTTCAAAATAGACGCGCATTACCAGATAGAGGCTATCGCTGACCTCTGCGGCAAGGGCTTTAAATCGACGCGCat     >  2:3172036/1‑93 (MQ=255)

                                                                                    cGACAGCGGTACGGGGTTTTTCAAAATAGACGCGCATTACCAGATAGAGGCTATCGCTGACCTCTGCGGCAAGGGCTTTAAATCGACGAGCat     >  2:641297/1‑93 (MQ=255)

                                                                                    cGACAGCGGTACGGGGTTTTTCAAAATAGACGCGCATTACCAGATAGAGGCTATCGCTGACCTCTGCGGCAAGGGCTTTAAATCGACGAGCat     <  1:2494948/93‑1 (MQ=255)

                                                                                     gACAGCGGTACGGGGTTTTTCAAAATAGACGCGCATTACCAGATAGAGGCTATCGCTGACCTCTGCGGCAAGGGCTTTAAATCGACGAGCata    <  1:5083415/93‑1 (MQ=255)

                                                                                      aCAGCGGTACGGGGTTTTTCAAAATAGACGCGCATTACCAGATAGAGGCTATCGCTGACCTCTGCGGCAAGGGCTTTAAATCGACGAGCatat   >  2:4766680/1‑93 (MQ=255)

                                                                                      aCAGCGGTACGGGGTTTTTCAAAATAGACGCGCATTACCAGATAGAGGCTATCGCTGACCTCTGCGGCAAGGGCTTTAAATCGACGAGCatat   >  1:2853278/1‑93 (MQ=255)

                                                                                      aCAGCGGTACGGGGTTTTTCAAAATAGACGCGCATTACCAGATAGAGGCTATCGCTGACCTCTGCGGCAAGGGCTTTAAATCGACGAGCatat   >  1:2679872/1‑93 (MQ=255)

                                                                                       cAGCGGTACGGGGTTTTTCAAAATAGACGCGCATTACCAGATAGAGGCTATCGCTGACCTCTGCGGCAAGGGCTTTAAATCGACGAGCATAtt  >  1:675233/1‑93 (MQ=255)

GCAGCAATTTACGCGCGATCTGCAGCCCGGCTTCTACATCAAAAGAGCCATCCATATGGGGATCGTTAATTAACCCTTTCCAGCCGACAGTGGTACGGGGTTTTTCAAAATAGACGCGCATTACCAGATAGAGGCTATCGCTGACCTCTGCGGCAAGGGCTTTAAATCGACGAGCATATT  >  NC_000913/2738777‑2738956

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 18 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

BRESEQ :: bam2aln output

AAGCAGCAATTTACGCGCGATCTGCAGCCCGGCTTCTACATCAAAAGAGCCATCCATATGGGGATCGTTAATTAACCCTTTCCAGCCGACAGTGGTACGGGGTTTTTCAAAATAGACGCGCATTACCAGATAGAGGCTATCGCTGACCTCTGCGGCAAGGGCTTTAAATCGACGAGCATATTCCA  >  NC_000913/2738775‑2738959

AAGCAGCAATTTACGCGCGATCTGCAGCCCGGCTTCTACATCAAAAGAGCCATCCATATGGGGATCGTTAATTAACCCTTTCCAGCCGACAGCG                                                                                             <  SRR4302537.5912443/94‑1 (MQ=60)

AAGCAGCAATTTACGCGCGATCTGCAGCCCGGCTTCTACATCAAAAGAGCCATCCATATGGGGATCGTTAATTAACCCTTTCCAGCCGACAGCG                                                                                             <  SRR4302537.4868762/94‑1 (MQ=60)

AAGCAGCAATTTACGCGCAATCTGCAGCCCGGCTTCTACATCAAAAGAGCCATCCATATGGGGATCGTTAATTAACCCTTTCCAGCCGACAGCG                                                                                             <  SRR4302537.3649364/94‑1 (MQ=60)

  CCAGCAATTTACGCGCGATCTGCAGCCCGGCTTCTACATCAAAAGAGCCATCCATATGGGGATCGTTAATTAACCCTTTCCAGCCGACAGTGGT                                                                                           <  SRR4302537.2074617/94‑1 (MQ=60)

   CAGCAATTTACGCGCGATCTGCAGCCCGGCTTCTACATCAAAAGAGCCATCCATATGGGGATCGTTAATTAACCCTTTCCAGCCGACAGCGGTA                                                                                          >  SRR4302537.1953715/1‑94 (MQ=60)

    AGCAATTTACGCGCGATCTGCAGCCCGGCTTCTACATCAAAAGAGCCATCCATATGGGGATCGTTAATTAACCCTTTCCAGCCGACAGCGGTAC                                                                                         >  SRR4302537.3681070/1‑94 (MQ=60)

    AGCAATTTACGCGCGATCTGCAGCCCGGCTTCTACATCAAAAGAGCCATCCATATGGGGATCGTTAATTAACCCTTTCCAGCCGACAGCGGTAC                                                                                         >  SRR4302537.5026217/1‑94 (MQ=60)

     GCAATTTACGCGCGATCTGCAGCCCGGCTTTTACATCAAAAGAGCCATCCATATGGGGATCGTTAATTAACCCTTTCCAGCCGACAGCGGTACG                                                                                        <  SRR4302537.4568764/94‑1 (MQ=60)

     GCAATTTACGCGCGATCTGCAGCCCGGCTTCTACATCAAAAGAGCCATCCATATGGGGATCGTTAATTAACCCTTTCCAGCCGACAGCGGTACG                                                                                        >  SRR4302537.1205709/1‑94 (MQ=60)

     GCAATTTACGCGCGATCTGCAGCCCGGCTTCTACATCAAAAGAGCCATCCATATGGGGATCGTTAATTAACCCTTTCCAGCCGACAGCGGTACG                                                                                        >  SRR4302537.2312419/1‑94 (MQ=60)

     GCAATTTACGCGCGATCTGCAGCCCGGCTTCTACATCAAAAGAGCCATCCATATGGGGATCGTTAATTAACCCTTTCCAGCCGACAGCGGTACG                                                                                        <  SRR4302537.1776196/94‑1 (MQ=60)

     GCAATTTACGCGCGATCTGCAGCACGGCTTCTACAGCAAAAGAGCCATCCATATGGGGATCGTTAATTAACCCTTTCCAGCCGACAGCGGTACG                                                                                        <  SRR4302537.3890964/94‑1 (MQ=60)

       AATTTACGCGCGATCTGCAGCCCGGCTTCTACATCAAAAGAGCCATCCATATGGGGATCGTTAATTAACCCTTTCCAGCCGACAGCGGTACGGG                                                                                      >  SRR4302537.4950429/1‑94 (MQ=60)

        ATTTACGCGCGATCTGCAGCCCGGCTTCTACATCAAAAGAGCCATCCATATGGGGATCGTTAATTAACCCTTTCCAGCCGACAGCGGTACGGGG                                                                                     >  SRR4302537.2534345/1‑94 (MQ=60)

        ATTTACGCGCGATCTGCAGCCCGGCTTCTACATCAAAAGAGCCATCCATATGGGGATCGTTAATTAACCCTTTCCAGCCGACAGCGGTACGGGG                                                                                     <  SRR4302537.757799/94‑1 (MQ=60)

          TTACGCGCGATCTGCAGCCCGGCTTCTACATCAAAAGAGCCATCCATATGGGGATCGTTAATTAACCCTTTCCAGCCGACAGCGGTACGGGGTT                                                                                   >  SRR4302537.4082830/1‑94 (MQ=60)

             CGCGCGATCTGCAGCCCGGCTTCTACATCAAAAGAGCCATCCATATGGGGATCGTTAATTAACCCTTTCCAGCCGACAGCGGTACGGGGTTTTT                                                                                <  SRR4302537.4035979/94‑1 (MQ=60)

              GCGCGATCTGCAGCCCGGCTTCTACATCAAAAGAGCCATCCATATGGGGATCGTTAATTAACCCTTTCCAGCCGACAGCGGTACGGGGTTTTTC                                                                               >  SRR4302537.4275386/1‑94 (MQ=60)

              GCGCGATCTGCAGCCCGGCTTCTACATCAAAAGAGCCATCCATATGGGGATCGTTAATTAACCCTTTCCAGCCGACAGCGGTACGGGGTTTTTC                                                                               >  SRR4302537.1952643/1‑94 (MQ=60)

              GCGCGATCTGCAGCCCGGCTTCTACATCAAAAGAGCCATCCATATGGGGATCGTTAATTAACCCTTTCCAGCCGACAGCGGTACGGGGTTTTTC                                                                               <  SRR4302537.76213/94‑1 (MQ=60)

               CGCGATCTGCAGCCCGGCTTCTACATCAAAAGAGCCATCCATATGGGGATCGTTAATTAACCCTTTCCAGCCGACAGCGGTACGGGGTTTTTCA                                                                              >  SRR4302537.2116903/1‑94 (MQ=60)

                GCGATCTGCAGCCCGGCTTCTACATCAAAAGAGCCATCCATATGGGGATCGTTAATTAACCCTTTCCAGCCGACAGCGGTACGGGGTTTTTCAA                                                                             >  SRR4302537.2172439/1‑94 (MQ=60)

                 CGATCTGCAGCCCGGCTTCTACATCAAAAGAGCCATCCATATGGGGATCGTTAATTAACCCTTTCCAGCCGACAGCGGTACGGGGTTTTTCAAA                                                                            >  SRR4302537.3867034/1‑94 (MQ=60)

                 CGATCTGCAGCCCGGCTTCTACATCAAAAGAGCCATCCATATGGGGATCGTTAATTAACCCTTTCCAGCCGACAGCGGTACGGGGTTTTTCAAA                                                                            >  SRR4302537.2156828/1‑94 (MQ=60)

                  GATCTGCAGCCCGGCTTCTACATCAAAAGAGCCATCCATATGGGGATCGTTCATTAACCCTTTCCAGCCGACAGCGGTACGGGGTTTTTCAAAA                                                                           >  SRR4302537.6006816/1‑94 (MQ=60)

                  GATCTGCAGCCCGGCTTCTACATCAAAAGAGCCATCCATATGGGGATCGTTAATTAACCCTTTCCAGCCGACAGCGGTACGGGGTTTTTCAAAA                                                                           >  SRR4302537.244104/1‑94 (MQ=60)

                    TCTGCAGCCCGGCTTCTACATCAAAAGAGCCATCCATATGGGGATCGTTAATTAACCCTTTCCAGCCGACAGCGGTACGGGGTTTTTCAAAATA                                                                         <  SRR4302537.1724410/94‑1 (MQ=60)

                     CTGCAGCCCGGCTTCTACATCAAAAGAGCCATCCATATGGGGATCGTTAATTAACCCTTTCCAGCCGACAGCGGTACGGGGTTTTTCAAAATAG                                                                        <  SRR4302537.3930849/94‑1 (MQ=60)

                       GCAGCCCGGCTTCTACATCAAAAGAGCCATCCATATGGGGATCGTTAATTAACCCTTTCCAGCCGACAGCGGTACGGGGTTTTTCAAAATAGAC                                                                      <  SRR4302537.1616826/94‑1 (MQ=60)

                        CAGCCCGGCTTCTACATCAAAAGAGCCATCCATATGGGGATCGTTAATTAACCCTTTCCAGCCGACAGCGGTACGGGGTTTTTCAAAATAGACG                                                                     >  SRR4302537.731218/1‑94 (MQ=60)

                        CAGCCCGGCTTCTACATCAAAAGAGCCATCCATATGGGGATCGTTAATTAACCCTTTCCAGCCGACAGCGGTACGGGGGTTTTCAAAATAGACG                                                                     >  SRR4302537.2529040/1‑94 (MQ=60)

                         AGCCCGGCTTCTACATCAAAAGAGCCATCCATATGGGGATCGTTAATTAACCCTTTCCAGCCGACAGCGGTACGGGGTTTTTCAAAATAGACGC                                                                    >  SRR4302537.2485755/1‑94 (MQ=60)

                         AGCCCGGCTTCTACATCAAAAGAGCCATCCATATGGGGATCGTTAATTAACCCTTTCCAGCCGACAGCGGTACGGGGGTTTTCAGAATAGACGC                                                                    >  SRR4302537.5534075/1‑94 (MQ=60)

                         AGCCCGGCTTCTACATCAAAAGAGCCATCCATATGGGGATCGTTAATTAACCCTTTCCAGCCGACAGCGGTACGGGGATTTTCAAAATAGACGC                                                                    >  SRR4302537.1608249/1‑94 (MQ=60)

                          GCCCGGCTTCTACATCAAAAGAGCCATCCATATGGTGATCGTTAATTAACCCTTTCCAGCCGACAGCGGTACGGGGTTTTTCAAAATAGACGCG                                                                   <  SRR4302537.6138556/94‑1 (MQ=60)

                          GCCCGGCTTCTACATCAAAAGAGCCATCCATATGGGGATCGTTAATTAACCCTTTCCAGCCGACAGCGGTACGGGGTTTTTCGAAATAGACGCG                                                                   >  SRR4302537.6476550/1‑94 (MQ=60)

                          GCCCGGCTTCTACATCAAAAGAGCCATCCATATGGGGATCGTTAATTAACCCTTTCCAGCCGACAGCGGTACGGGGTTTTTCAAAATAGACGCG                                                                   <  SRR4302537.5362351/94‑1 (MQ=60)

                          GCCCGGCTTCTACATCAAAAGAGCCATCCATATGGGGATCGTTAATTAACCCTTTCCAGCCGACAGCGGTACGGGGTTTTTCAAAATAGACGCG                                                                   <  SRR4302537.6138555/94‑1 (MQ=60)

                          GCCCGGCTTCTACATCAAAAGAGCCATCCATATGGGGATCGTTAATTAACCCTTTCCAGCCGACAGCGGTACGGGGTTTTACAAAATAGACGCG                                                                   <  SRR4302537.4450018/94‑1 (MQ=60)

                             CGGCTTCTACATCAAAAGAGCCATCCATATGGGGATCGTTAATTAACCCTTTCCAGCCGACAGCGGTACGGGGTTTTTCAAAATAGACGCGCAT                                                                >  SRR4302537.1989788/1‑94 (MQ=60)

                             CGGCTTCTACATCAAAAGAACCATCCATATGGGGATCGTTAATTAACCCTTTCCAGCCGACAGCGGTACGGGGTTTTTCAAAATAGACGCGCAT                                                                >  SRR4302537.2170363/1‑94 (MQ=60)

                              GGCTTCTACATCAAAAGAGCCATCCATATGGGGATCGTTAATTAACCCTTTCCAGCCGACAGCGGTACGGGGTTTTTCAAAATAGACGCGCATT                                                               >  SRR4302537.3462823/1‑94 (MQ=60)

                              GGCTTCTACATCAAAAGAGCCATCCATATGGGGATCGTTAATTAACCCTTTCCAGCCGACAGCGGTACGGGGTTTTTCAAAATAGACGCGCATT                                                               >  SRR4302537.2594859/1‑94 (MQ=60)

                               GCTTCTACATCAAAAGAGCCATCCATATGGGGATCGTTAATTAACCCTTTCCAGCCGACAGCGGTACGGGGTTTTTCAAAATAGACGCGCATTA                                                              >  SRR4302537.1477468/1‑94 (MQ=60)

                                CTTCTACATCAAAAGAGCCATCCATATGGGGATCGTTAATTAACCCTTTCCAGCCGACAGCGGTACGGGGTTTTTCAAAATAGACGCGCATTAC                                                             >  SRR4302537.1628336/1‑94 (MQ=60)

                                CTTCTACATCAAAAGAGCCATCCATATGGGGATCGTTAATTAACCCTTTCCAGCCGACAGCGGTACGGGGTTTTTCAAAATAGACGCGCATTAC                                                             >  SRR4302537.5598082/1‑94 (MQ=60)

                                 TTCTACATCAAAAGAGCCATCCATATGGGGATCGTTAATTAACCCTTTCCAGCCGACAGCGGTACGGGGTTTTTCAAAATAGACGCGCATTACC                                                            <  SRR4302537.4550167/94‑1 (MQ=60)

                                 TTCTACATCAAAAGAGCCATCCATATGGGGATCGTTAATTAACCCTTTCCAGCCGACAGCGGTAAGGGGCTTTTCAAAATAGACGCGCATTACC                                                            >  SRR4302537.4696233/1‑94 (MQ=60)

                                  TCTACATCAAAAGAGCCATCCATATGGGGATCGTTAATTAACCCTTTCCAGCCGACAGCGGTACGGGGTTTTTCAAAATAGACGCGCATTACCA                                                           >  SRR4302537.2926260/1‑94 (MQ=60)

                                  TCTACATCAAAAGAGCCATCCATATGGGGATCGTTAATTAACCCTTTCCAGCCGACAGCGGTACGGGGGTTTTCAAAATAGACGCGCATTAACA                                                           >  SRR4302537.2332846/1‑94 (MQ=60)

                                   CTACATCAAAAGAGCCATCCATATGGGGATCGTTAATTAACCCTTTCCAGCCGACAGCGGTACGGGGTTTTTCAAAATAGACGCGCATTACCAG                                                          <  SRR4302537.4561555/94‑1 (MQ=60)

                                    TACATCAAAAGAGCCATCCATATGGGGATCGTTAATTAACCCTTTCCAGCCGACAGCGGTACGGGGTTTTTCAAAATAGACGCGCATTACCAGA                                                         <  SRR4302537.5000536/94‑1 (MQ=60)

                                      CATCAAAAGAGCCATCCATATGGGGATCGTTAATTAACCCTTTCCAGCCGACAGCGGTACGGGGTTTTTCAAAATAGACGCGCATTACCAGATA                                                       <  SRR4302537.2280033/94‑1 (MQ=60)

                                      CATCAAAAGAGCCATCCATATGGGGATCGTTAATTAACCCTTTCCAGCCGACAGCGGTACGGGGTTTTTCAAAATAGACGCGCATTACCAGATA                                                       >  SRR4302537.614329/1‑94 (MQ=60)

                                       ATCAAAAGAGCCATCCATATGGGGATCGTTAATTAACCCTTTCCAGCCGACAGCGGTACGGGGTTTTTCAAAATAGACGCGCATTACCAGATAG                                                      >  SRR4302537.623580/1‑94 (MQ=60)

                                       ATAAAAAGAGCCATCCATATGGGTATCGTTAATTAACCCTTTCCAGCCGACAGCGGTACGGGGTTTTTCAAAATAGACGCGCATTACCAGATAG                                                      <  SRR4302537.4679988/94‑1 (MQ=60)

                                        TCAAAAGAGCCATCCATATGGGGATCGTTAATTAACCCTTTCCAGCCGACAGCGGTACGGGGTTTTTCAAAATAGACGCGCATTACCAGATAGA                                                     >  SRR4302537.3492964/1‑94 (MQ=60)

                                          AAAAGAGCCATCCATATGGGGATCGTTAATTAACCCTTTCCAGCCGACAGCGGTACGGGGTTTTTCAAAATAGACGCGCATTACCAGATAGAGG                                                   <  SRR4302537.2315818/94‑1 (MQ=60)

                                           AAAGAGCCATCCATATGGGGATCGTTAATTAACCCTTTCCAGCCGACAGCGGTACGGGGTTTTTCAAAATAGACGCGCATTACCAGATAGAGGC                                                  <  SRR4302537.4201617/94‑1 (MQ=60)

                                           AAAGAGCCATCCATATGGGGATCGTTAATTAACCCTTTCCAGCCGACAGCGGTACGGGGTTTTTCAAAATAGACGCGCATTACCAGATAGAGGC                                                  <  SRR4302537.1054972/94‑1 (MQ=60)

                                            AAGAGCCATCCATATGGGGATCGTTAATTAACCCTTTCCAGCCGACAGCGGTACGGGGTTTTTCAAAATAGACGCGCATTACCAGATAGAGGCT                                                 <  SRR4302537.923574/94‑1 (MQ=60)

                                            AAGAGCCATCCATATGGGGATCGTTAATTAACCCTTTCCAGCCGACAGCGGTACGGGGTTTTTCAAAATAGACGCGCATTACCAGATAGAGGCT                                                 >  SRR4302537.1051636/1‑94 (MQ=60)

                                              GAGCCATCCATATGGGGATCGTTAATTAACCCTTTCCAGCCGACAGCGGTACGGGGTTTTTCAAAATAGACGCGCATTACCAGATAGAGGCTAT                                               <  SRR4302537.3047053/94‑1 (MQ=60)

                                               AGCCATCCATATGGGGATCGTTAATTAACCCTTTCCAGCCGACAGCGGTACGGGGTTTTTCAAAATAGACGCGCATTACCAGATAGAGGCTATC                                              >  SRR4302537.5041616/1‑94 (MQ=60)

                                               AGCCATCCATATGGGGATCGTTAATTAACCCTTTCCAGCCGACAGCGGTACGGGGTTTTTCAAAATAGACGCGCATTACCAGATAGAGGCTATC                                              <  SRR4302537.1421133/94‑1 (MQ=60)

                                                GCCATCCATATGGGGATCGTTAATTAACCCTTTCCAGCCGACAGCGGTACGGGGTTTTTCAAAATAGACGCGCATTACCAGATAGAGGCTATCG                                             <  SRR4302537.1099640/94‑1 (MQ=60)

                                                GCCATCCATATGGGGATCGTTAATTAACCCTTTCCAGCCGACAGCGGTACGGGGTTTTTCAAAATAGACGCGCATTACCAGATAGAGGCTATCG                                             <  SRR4302537.3553031/94‑1 (MQ=60)

                                                  CATCCATATGGGGATCGTTAATTAACCCTTTCCAGCCGACAGCGGTACGGGGTTTTTCAAAATAGACGCGCATTACCAGATAGAGGCTATCGCT                                           <  SRR4302537.4696177/94‑1 (MQ=60)

                                                   ATCCATATGGGGATCGTTAATTAACCCTTTCCAGCCGACAGCGGTACGGGGTTTTTCAAAATAGACGCGCATTACCAGATAGAGGCTATCGCTG                                          >  SRR4302537.38117/1‑94 (MQ=60)

                                                     CCATATGGGGATCGTTAATTAACCCTTTCCAGCCGACAGCGGTACGGGGTTTTTCAAAATAGACGCGCATTACCAGATAGAGGCTATCGCTGAC                                        >  SRR4302537.3987331/1‑94 (MQ=60)

                                                      CATATGGGTATCGTTAATTAACCCTTTCCAGCCGACAGCGGTACGGGGTTTTTCAAAATAGACGCGCATTACCAGATAGAGGCTATCGCTGACC                                       <  SRR4302537.5684922/94‑1 (MQ=60)

                                                      CATATGGGGCTCGTTAATTAACCCTTTCCAGCCGACAGCGGTACGGGGTTTTTCAAAATAGACGCGCATTACCATATATAGGATATCGCTGACT                                       >  SRR4302537.5151431/1‑94 (MQ=60)

                                                      CATATGGGGATCGTTAATTAACCCTTTCCAGCCGACAGCGGTACGGGGTTTTTCAAAATAGACGCGCATTACCAGATAGAGGCTATCGCTGACC                                       >  SRR4302537.5430401/1‑94 (MQ=60)

                                                      CATATGGGGATCGTTAATTAACCCTTTCCAGCCGACAGCGGTACGGGGTTTTTCAAAATAGACGCGCATTACCAGATAGAGGCTATCGCTGACC                                       >  SRR4302537.4146827/1‑94 (MQ=60)

                                                       ATATGGGGATCGTTAATTAACCCTTTCCAGCCTACAGCGGTACGGGGTTTTTCAAAATAGACGCGCATTACCAGATAGAGGCTATCGCTGACCT                                      >  SRR4302537.4165333/1‑94 (MQ=60)

                                                       ATATGGGGATCGTTAATTAACCCTTTCCAGCCGACAGCGGTACGGGGTTTTTCAAAATAGACGCGCATTACCAGATAGAGGCTATCGCTGACCT                                      >  SRR4302537.6408438/1‑94 (MQ=60)

                                                       ATATGGGGATCGTTAATTAACCCTTTCCAGCCGACAGCGGTACGGGGTTTTTCAAAATAGACGCGCATTACCAGATAGAGGCTATCGCTGACCT                                      >  SRR4302537.193004/1‑94 (MQ=60)

                                                         ATGGGGATCGTTAATTAACCCTTTCCAGCCTACAGCGGTACGGGGTTTTTCAAAATAGACGCGCATTACCAGATAGAGGCTATCGCTGACCTCT                                    >  SRR4302537.5560570/1‑94 (MQ=60)

                                                         ATGGGGATCGTTAATTAACCCTTTCCAGCCGACAGCGGTACGGGGTTTTTCAAAATAGACGCGCATTACCAGATAGAGGCTATCGCTGACCTCT                                    <  SRR4302537.5694697/94‑1 (MQ=60)

                                                         ATGGGGATCGTTAATTAACCCTTTCCAGCCGACAGCGGTACGGGGTTTTTCAAAATAGACGCGCATTACCAGATAGAGGCTATCGCTGACCTCT                                    >  SRR4302537.6003412/1‑94 (MQ=60)

                                                          TGGGGATCGTTAATTAACCCTTTCCAGCCGACAGCGGTACGGGGTTTTTCAAAATAGACGCGCATTACCAGATAGAGGCTATCGCTGACCTCTG                                   <  SRR4302537.2017260/94‑1 (MQ=60)

                                                           GGGGATCGTTAATTAACCCTTTCCAGCCGACAGCGGTACGGGGTTTTTCAAAATAGACGCGCATTACCAGATAGAGGCTATCGCTGACCTCTGC                                  <  SRR4302537.5115143/94‑1 (MQ=60)

                                                           GGGGATCGTTAATTAACCCTTTCCAGCCGACAGCGGTACGGGGTTTTTCAAAATAGACGCGCATTACCAGATAGAGGCTATCGCTGACCTCTGC                                  >  SRR4302537.300283/1‑94 (MQ=60)

                                                            GGGATCGTTAATTAACCCTTTCCAGCCGACAGCGGTACGGGGTTTTTCAAAATAGACGCGCATTACCAGATAGAGGCTATCGCTGACCTCTGCG                                 >  SRR4302537.237412/1‑94 (MQ=60)

                                                            GGGATCGTTAATTAACCCTTTCCAGCCGACAGCGGTACGGGGTTTTTCAAAATAGACGCGCATTACCAGATAGAGGCTATCGCTGACCTCTGCG                                 <  SRR4302537.6505136/94‑1 (MQ=60)

                                                             GGATCGTTAATTAACCCTTTCCAGCCGACAGCGGTACGGGGTTTTTCAAAATAGACGCGCATTACCAGATAGAGGCTATCGCTGACCTCTGCGG                                >  SRR4302537.5651970/1‑94 (MQ=60)

                                                             GGATCGTTAATTAACCCTTTCCAGCCGACAGCGGTACGGGGTTTTTCAAAATAGACGCGCATTACCAGATAGAGGCTATCGCTGACCTCTGCGG                                >  SRR4302537.1575643/1‑94 (MQ=60)

                                                              GATCGTTAATTAACCCTTTCCAGCCGACAGCGGTACGGGGTTTTTCAAAATAGACGCGCATTACCAGATAGAGGCTATCGCTGACCTCTGCGGC                               >  SRR4302537.3100524/1‑94 (MQ=60)

                                                              GATCGTTAATTAACCCTTTCCAGCCGACAGCGGTACGGGGTTTTTCAAAATAGACGCGCATTACCAGATAGAGGCTATCGCTGACCTCTGCGGC                               >  SRR4302537.3236463/1‑94 (MQ=60)

                                                               ATCGTTAATTAACCCTTTCCAGCCGACAGCGGTACGGGGTTTTTCAAAATAGACGCGCATTACCAGATAGAGGCTATCGCTGACCTCTGCGGCA                              >  SRR4302537.492055/1‑94 (MQ=60)

                                                               ATCGTTAATTAACCCTTTCCAGCCGACAGCGGTACGGGGTTTTTCAAAATAGACGCGCATTACCAGATAGAGGCTATCGCTGACCTCTGCGGCA                              >  SRR4302537.492056/1‑94 (MQ=60)

                                                               ATCGTTAATTAACCCTTTCCAGCCGACAGCGGTACGGGGTTTTTCAAAATAGACGCGCATTACCAGATAGAGGCTATCGCTGACCTCTGCGGCA                              >  SRR4302537.6519186/1‑94 (MQ=60)

                                                               ATCGTTAATTAACCCTTTCCAGCCGACAGCGGTACGGGGTTTTTCAAAATAGACGCGCATTACCAGATAGAGGCTATCGCTGACCTCTGCGGCA                              >  SRR4302537.1495140/1‑94 (MQ=60)

                                                               ATCGTTAATTAACCCTTTCCAGCCGACAGCGGTACGGGGTTTTTCAAAATAGACGCGCATTACCAGATAGAGGCTATCGCTGACCTCTGCGGCA                              <  SRR4302537.3897917/94‑1 (MQ=60)

                                                               ATCGTTAATTAACCCTTTCCAGCCGACAGCGGTACGGGGTTTTTCAAAATAGACGCGCATTACCAGATAGAGGCTATCGCTGACCTCTGCGGCA                              >  SRR4302537.1956234/1‑94 (MQ=60)

                                                                 CGTTAATTAACCCTTTCCAGCCGACAGTGGTACGGGGTTTTTCAAAATAGACGCGCATTACCAGATAGAGGCTATCGCTGACCTCTGCGGCAAG                            <  SRR4302537.1954512/94‑1 (MQ=60)

                                                                   TTAATTAACCCTTTCCAGCCGACAGCGGTACGGGGTTTTTCAAAATAGACGCGCGTTACCAGATAGAGGCTATCGCTGACCTCTGCGGCAAGGG                          <  SRR4302537.4093849/94‑1 (MQ=60)

                                                                    TAATTAACCCTTTCCAGCCGACAGCGGTACGGGGTTTTTCAAAATAGACGCGCATTACCAGATAGAGGCTATCGCTGACCTCTGCGGCAAGGGC                         >  SRR4302537.3863224/1‑94 (MQ=60)

                                                                    TAATTAACCCTTTCCAGCCGACAGCGGTACGGGGTTTTTCAAAATAGACGCGCATTACCAGATAGAGGCTATCGCTGACCTCTGCGGCAAGGGC                         <  SRR4302537.4596749/94‑1 (MQ=60)

                                                                     AATTAACCCTTTCCAGCCGACAGCGGTACGGGGTTTTTCAAAATAGACGCGCATTACCAGATAGAGGCTATCGCTGACCTCTGCGGCAAGGGCT                        <  SRR4302537.285816/94‑1 (MQ=60)

                                                                     AATTAACCCTTTCCAGCCGACAGCGGTACGGGGTTTTTCAAAATAGACGCGCATTACCAGATAGAGGCTATCGCTGACCTCTGCGGCAAGGGCT                        >  SRR4302537.2930264/1‑94 (MQ=60)

                                                                       TTAACCCTTTCCAGCCGACAGCGGTACGGGGTTTTTCAAAATAGACGCGCATTACCAGATAGAGGCTATCGCTGACCTCTGCGGCAAGGGCTTT                      >  SRR4302537.4103941/1‑94 (MQ=60)

                                                                       TTAACCCTTTCCAGCCGACAGCGGTACGGGGTTTTTCAAAATAGACGCGCATTACCAGATAGAGGCTATCGCTGACCTCTGCGGCAAGGGCTTT                      <  SRR4302537.893540/94‑1 (MQ=60)

                                                                       TTAACCCTTTCCAGCCGACAGCGGTACGGGGTTTTTCAAAATAGACGCGCATTACCAGATAGAGGCTATCGCTGACCTCTGCGGCAAGGGCTTT                      <  SRR4302537.6224225/94‑1 (MQ=60)

                                                                        TAACCCTTTCCAGCCGACAGCGGTACGGGGTTTTTCAAAATAGACGCGCATTACCAGATAGAGGCTATCGCTGACCTCTGCGGCAAGGGCTTTA                     >  SRR4302537.77366/1‑94 (MQ=60)

                                                                        TAACCCTTTCCAGCCGACAGCGGTACGGGGTTTTTCAAAATAGACGCGCATTACCAGATAGAGGCTATCGCTGACCTCTGCGGCAAGGGCTTTA                     <  SRR4302537.5785472/94‑1 (MQ=60)

                                                                         AACCCTTTCCAGCCGACAGCGGTACGGGGTTTTTCAAAATAGACGCGCATTACCAGATAGAGGCTATCGCTGACCTCTGCGGCAAGGGCTTTCA                    >  SRR4302537.3153625/1‑94 (MQ=60)

                                                                         AACCCTTTCCAGCCGACAGCGGTACGGGGTTTTTCAAAATAGACGCGCATTACCAGATAGAGGCTATCGCTGACCTCTGCGGCAAGGGCTTTAA                    >  SRR4302537.237678/1‑94 (MQ=60)

                                                                         AACCCTTTCCAGCCGACAGCGGTACGGGGTTTTTCAAAATAGACGCGCATTACCAGATAGAGGCTATCGCTGACCTCTGCGGCAAGGGCTTTAA                    <  SRR4302537.1887970/94‑1 (MQ=60)

                                                                         AACCCTTTCCAGCCGACAGCGGTACGGGGCTTTTCAAAATAGACGCGCATTACCAGATAGAGGCTATCGCTGACCTCTGCGGCAAGGGCTTTAA                    >  SRR4302537.64428/1‑94 (MQ=60)

                                                                          ACCCTTTCCAGCCGACAGCGGTACGGGGTTTTTCATAATAGACGCGCATTACCAGATAGAGGCTATCGCTGACCTCTGCGGCAAGGGCTTTAAA                   >  SRR4302537.4400495/1‑94 (MQ=60)

                                                                          ACCCTTTCCAGCCGACAGCGGTACGGGGTTTTTCAAAATAGACGCGCATTACCAGATAGAGGCTATCGCTGACCTCTGCGGCAAGGGCTTTAAA                   <  SRR4302537.6406992/94‑1 (MQ=60)

                                                                          ACCCTTTCCAGCCGACAGCGGTACGGGGTTTTTCAAAATAGACGCGCATTACCAGATAGAGGCTATCGCTGACCTCTGCGGCAAGGGCTTTAAA                   <  SRR4302537.5415773/94‑1 (MQ=60)

                                                                          ACCCTTTCCAGCCGACAGCGGTACGGGGTTTTTCAAAATAGACGCGCATTACCAGATAGAGGCTATCGCTGACCTCTGCGGCAAGGGCTTTAAA                   >  SRR4302537.5123352/1‑94 (MQ=60)

                                                                          ACCCTTTCCAGCCGACAGCGGTACGGGGTTTTTCAAAATAGACGCGCATTACCAGATAGAGGCTATCGCTGACCTCTGCGGCAAGGGCTTTAAA                   >  SRR4302537.677007/1‑94 (MQ=60)

                                                                          ACCCTTTCCAGCCGACAGCGGTACGGGGTTTTTCAAAATAGACGCGCATTACCAGATAGAGGCTATCGCTGACCTCTGCGGCAAGGGCTTTAAA                   <  SRR4302537.3703290/94‑1 (MQ=60)

                                                                            CCTTTCCAGCCGACAGCGGTACGGGGTTTTTCAAAATAGACGCGCATTACCAGATAGAGGCTATCGCTGACCTCTGCGGCAAGGGCTTTAAATC                 <  SRR4302537.6347026/94‑1 (MQ=60)

                                                                            CCTTTCCAGCCGACAGCGGTACGGGGTTTTTCAAAATAGACGCGCATTACCAGATAGAGGCTATCGCTGACCTCTGCGGCAAGGGCTTTAAATC                 <  SRR4302537.1434242/94‑1 (MQ=60)

                                                                            CCTTTCCAGCCGACAGCGGTACGGGGTTTTTCAAAATAGACGCGCATTACCAGATAGAGGCTATCGCTGACCTCTGCGGCAAGGGCTTTAAATC                 >  SRR4302537.483356/1‑94 (MQ=60)

                                                                             CTTTCCAGGCGACAGCGGTACGGGGTTTTTCAAAATAGACGCGCATTACCAGATAGAGGCTATCGCTGACCTCTGCGGCAAGGGCTTTAAATCG                <  SRR4302537.1795145/94‑1 (MQ=60)

                                                                             CTTTCCAGCCGACAGCGGTACGGGGTTTTTCAAAATAGACGCGCATTACCAGATAGAGGCTATCGCTGACCTCTGCGGCAAGGGCTTTAAATCG                >  SRR4302537.3995561/1‑94 (MQ=60)

                                                                              TTTCCAGCCGACAGCGGTACGGAGTTTTTCAAAATAGACGCGCATTACCAGATAGAGGCTATCGCTGACCTCTGCGGCAAGGGCTTTAAATCGA               <  SRR4302537.3872138/94‑1 (MQ=60)

                                                                               TTCCAGCCGACAGCGGTACGGGGTTTTTCAAAATAGACGCGCATTACCAGATAGAGGCTATCGCTGACCTCTGCGGCAAGGGCTTTAAATCGAC              <  SRR4302537.4551931/94‑1 (MQ=60)

                                                                                TCCAGCCGACAGCGGTACGGGGTTTTTCAAAATAGACGCGCATTACCAGATAGAGGCTATCGCTGACCTCTGCGGCAAGGGCTTTAAATCGACG             <  SRR4302537.406301/94‑1 (MQ=60)

                                                                                 CCAGCCGACAGCGGTACGGGGTTTTTCAAAATAGACGCGCATTACCAGATAGAGGCTATCGCTGACCTCTGCGGCAAGGGCTTTAAATCGACGA            >  SRR4302537.2053884/1‑94 (MQ=60)

                                                                                  CAGCCGACAGCGGTACGGGGTTTTTCAAAATAGACGCGCATTACCAGATAGAGGCTATCGATGACCTCTGCGGCACGGGCTTTAAATCGACGAG           >  SRR4302537.2570834/1‑94 (MQ=60)

                                                                                    GCCGACAGCGGTACGGGGTTTTTCAAAATAGACGCGCATTACCAGATAGAGGCTATCGCTGACCTCTGCGGCAAGGGCTTTAAATCGACGAGCA         >  SRR4302537.5879675/1‑94 (MQ=60)

                                                                                    GCCGACAGCGGTACGGGGTTTTTCAAAATAGACGCGCATTACCAGATAGAGGCTATCGCTGACCTCTGCGCCAAGGGCTTTAAATCGACGAGCA         <  SRR4302537.6043920/94‑1 (MQ=60)

                                                                                      CGACAGCGGTACGGGGTTTTTCAAAATAGACGCGCATTACCAGATAGAGGCTATCGCTGACCTCTGCGGCAAGGGCTTTAAATCGACGAGCATA       <  SRR4302537.2512514/94‑1 (MQ=60)

                                                                                      CGACAGCGGTACGGGGTTTTTCAAAATAGACGCGCATTACCAGATAGAGGCTATCGCTGACCTCTGCGGCAAGGGCTTTAAATCGACGAGCATA       <  SRR4302537.4299520/94‑1 (MQ=60)

                                                                                       GACAGCGGTACGGGGTTTTTCAAAATAGACGCGCATTACCAGATAGAGGCTATCGCTGACCTCTGCGGCAAGGGCTTTAAATCGACGAGCATAT      >  SRR4302537.2873272/1‑94 (MQ=60)

                                                                                       GACAGCGGTACGGGGTTTTTCAAAATAGACGCGCATTACCAGATAGAGGCTATCGCTGACCTCTGCGGCAAGGGCTTTAAATCGACGAGCATAT      >  SRR4302537.2698585/1‑94 (MQ=60)

                                                                                       GACAGCGGTACGGGGTTTTTCAAAATAGACGCGCATTACCAGATAGAGGCTATCGCTGACCTCTGCGGCAAGGGCTTTAAATCGACGAGCATAT      <  SRR4302537.5118401/94‑1 (MQ=60)

                                                                                        ACAGCGGTACGGGGTTTTTCAAAATAGACGCGCATTACCAGATAGAGGCTATCGCTGACCTCTGCGGCAAGGGCTTTAAATCGACGAGCATATT     <  SRR4302537.4106516/94‑1 (MQ=60)

                                                                                        ACAGCGGTACGGGGTTTTTCAAAATAGACGCGCATTACCAGATAGAGGCTATCGCTGACCTCTGCGGCAAGGGCTTTAAATCGACGAGCATATT     >  SRR4302537.679855/1‑94 (MQ=60)

                                                                                         CAGCGGTACGGGGTTTTTCAAAATAGACGCGCATTACCAGATAGAGGCTATCGCTGACCTCTGCGGCAAGGGCTTTAAATCGACGAGCATATTC    >  SRR4302537.3391234/1‑94 (MQ=60)

                                                                                         CAGCGGTACGGGGTTTTTCAAAATAGACGCGCATTACCAGATAGAGGCTATCGCTGACCTCTGCGGCAAGGGCTTTAAATCGACGAGCATATTC    <  SRR4302537.2524060/94‑1 (MQ=60)

                                                                                          AGCGGTACGGGGTTTTTCAAAATAGACGCGCATTACCAGATAGAGGCTATCGCTGACCTCTGCGGCAAGGGCTTTAAATCGACGAGCATATTCC   >  SRR4302537.751947/1‑94 (MQ=60)

                                                                                           GCGGTACGGGGTTTTTCAAAATAGACGCGCATTACCAGATAGAGGCTATCGCTGACCTCTGCGGCAAGGGCTTTAAATCGACGAGCATATTCCA  <  SRR4302537.3675330/94‑1 (MQ=60)

                                                                                           GCGGTACGGGGTTTTTCAAAATAGACGCGCATTACCAGATAGAGGCTATCGCTGACCTCTGCGGCAAGGGCTTTAAATCGACGAGCATATTCCA  >  SRR4302537.349427/1‑94 (MQ=60)

AAGCAGCAATTTACGCGCGATCTGCAGCCCGGCTTCTACATCAAAAGAGCCATCCATATGGGGATCGTTAATTAACCCTTTCCAGCCGACAGTGGTACGGGGTTTTTCAAAATAGACGCGCATTACCAGATAGAGGCTATCGCTGACCTCTGCGGCAAGGGCTTTAAATCGACGAGCATATTCCA  >  NC_000913/2738775‑2738959

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 0 ≤` `ATCG/ATCG` `< 7 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`