Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A1 F1 I2 R1	882	254.4	12957588	98.0%	12698436	93.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,762,061	+C	coding (1114/1617 nt)	yfjL ←	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,762,057	1	.	C	97.8%	784.4 / 7.8	232	coding (1118/1617 nt)	yfjL	hypothetical protein
Reads supporting (aligned to +/- strand): ref base . (2/3); new base C (118/109); total (120/112)
Fisher's exact test for biased strand distribution p-value = 6.74e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.86e-01

Only 100 of 261 total aligned reads displayed.

AATATCAGTCACCAACTCGCTGAAGCCAATCCAAAGCTGATCACCTTCTGGATCGCGGCGAACGATATGCACGTTTTCAAGAATTTTTTCGG‑CCCCTGCTTTTTCCACGTATAGCATGCAAGGAATAGGCTGGCTTTCGTGGTGCTGCCGGATCGTTGCGTGCAACAGCACTCGACCC  >  NC_000913/2761966‑2762143

 atatCAGTCACCAACTCGCTGAAGCCAATCCAAAGCTGATCACCTTCTGGATCGCGGCGAACGATATGCACGTTTTCAAGAATTTTTTCGG‑cc                                                                                      >  2:493734/1‑93 (MQ=255)

    tCAGTCACCAACTCGCTGAAGCCAATCCAAAGCTGATCACCTTCTGGATCGCGGCGAACGATATGCACGTTTTCAAGAATTTTTTCGG‑ccccc                                                                                   <  2:2264404/93‑2 (MQ=255)

    tCAGTCACCAACTCGCTGAAGCCAATCCAAAGCTGATCACCTTCTGGATCGCGGCGAACGATATGCACGTTTTCAAGAATTTTTTCGG‑ccccc                                                                                   >  1:981926/1‑92 (MQ=255)

    tCAGTCACCAACTCGCTGAAGCCAATCCAAAGCTGATCACCTTCTGGATCGCGGCGAACGATATGCACGTTTTCAAGAATTTTTTCGG‑ccccc                                                                                   >  1:3598796/1‑92 (MQ=255)

    tCAGTCACCAACTCGCTGAAGCCAATCCAAAGCTGATCACCTTCTGGATCGCGGCGAACGATATGCACGTTTTCAAGAATTTTTTCGG‑ccccc                                                                                   >  2:3505015/1‑92 (MQ=255)

    tCAGTCACCAACTCGCTGAAGCCAATCCAAAGCTGATCACCTTCTGGATCGCGGCGAACGATATGCACGTTTTCAAGAATTTTTTCGG‑ccccc                                                                                   <  1:1921316/93‑2 (MQ=255)

     cAGTCACCAACTCGCTGAAGCCAATCCAAAGCTGATCACCTTCTGGATCGCGGCGAACGATATGCACGTTTTCAAGAATTTTTTCGG‑ccccct                                                                                  <  1:3461272/93‑3 (MQ=255)

      aGTCACCAACTCGCTGAAGCCAATCCAAAGCTGATCACCTTCTGGATCGCGGCGAACGATATGCACGTTTTCAAGAATTTTTTCGG‑ccccctg                                                                                 <  1:5877710/93‑4 (MQ=255)

       gTCACCAACTCGCTGAAGCCAATCCAAAGCTGATCACCTTCTGGATCGCGGCGAACGATATGCACGTTTTCAAGAATTTTTTCGG‑ccccctgc                                                                                >  2:3263284/1‑89 (MQ=255)

       gTCACCAACTCGCTGAAGCCAATCCAAAGCTGATCACCTTCTGGATCGCGGCGAACGATATGCACGTTTTCAAGAATTTTTTCGG‑ccccctgc                                                                                <  1:4104770/93‑5 (MQ=255)

          aCCAACTCGCTGAAGCCAATCCAAAGCTGATCACCTTCTGGATCGCGGCGAACGATATGCACGTTTTCAAGAATTTTTTCGGCCCCCTGCttt                                                                              <  2:1643450/93‑1 (MQ=255)

          aCCAACTCGCTGAAGCCAATCCAAAGCTGATCACCTTCTGGATCGCGGCGAACGATATGCACGTTTTCAAGAATTTTTTCGGCCCCCTGCttt                                                                              <  1:2575879/93‑1 (MQ=255)

           ccAACTCGCTGAAGCCAATCCAAAGCTGATCACCTTCTGGATCGCGGCGAACGATATGCACGTTTTCAAGAATTTTTTCGGCCCCCTGCtttt                                                                             >  2:5715867/1‑93 (MQ=255)

            cAACTCGCTGAAGCCAATCCAAAGCTGATCACCTTCTGGATCGCGGCGAACGATATGCACGTTTTCAAGAATTTTTTCGGCCCCCTGCttttt                                                                            <  2:1379993/93‑1 (MQ=255)

            cAACTCGCTGAAGCCAATCCAAAGCTGATCACCTTCTGGATCGCGGCGAACGATATGCACGTTTTCAAGAATTTTTTCGGCCCCCTGCttttt                                                                            <  2:740274/93‑1 (MQ=255)

            cAACTCGCTGAAGCCAATCCAAAGCTGATCACCTTCTGGATCGCGGCGAACGATATGCACGTTTTCAAGAATTTTTTCGGCCCCCTGCttttt                                                                            >  2:3621451/1‑93 (MQ=255)

             aaCTCGCTGAAGCCAATCCAAAGCTGATCACCTTCTGGATCGCGGCGAACGATATGCACGTTTTCAAGAATTTTTTCGGCCCCCTGCTTTTTc                                                                           >  2:5190260/1‑93 (MQ=255)

             aaCTCGCTGAAGCCAATCCAAAGCTGATCACCTTCTGGATCGCGGCGAACGATATGCACGTTTTCAAGAATTTTTTCGGCCCCCTGCTTTTTc                                                                           >  1:616416/1‑93 (MQ=255)

              aCTCGCTGAAGCCAATCCAAAGCTGATCACCTTCTGGATCGCGGCGAACGATATGCACGTTTTCAAGAATTTTTTCGGCCCCCTGCTTTTTcc                                                                          >  1:2457714/1‑93 (MQ=255)

                tCGCTGAAGCCAATCCAAAGCTGATCCCCTTCTGGAGCGCGGCGAACGATATGCACGTTTTCAAGAATTTTTTCGGCCCCCTGCTTTTTCCAc                                                                        <  1:1690224/93‑1 (MQ=255)

                tCGCTGAAGCCAATCCAAAGCTGATCACCTTCTGGATCGCGGCGAACGATATGCACGTTTTCAAGAATTTTTTCGGCCCCCTGCTTTTTCCAc                                                                        >  2:474288/1‑93 (MQ=255)

                tCGCTGAAGCCAATCCAAAGCTGATCACCTTCTGGATCGCGGCGAACGATATGCACGTTTTCAAGAATTTTTTCGGCCCCCTGCTTTTTCCAc                                                                        >  1:6418175/1‑93 (MQ=255)

                tCGCTGAAGCCAATCCAAAGCTGATCACCTTCTGGATCGCGGCGAACGATATGCACGTTTTCAAGAATTTTTTCGGCCCCCTGCTTTTTCCAc                                                                        >  1:2729240/1‑93 (MQ=255)

                tCGCTGAAGCCAATCCAAAGCTGATCACCTTCTGGATCGCGGCGAACGATATGCACGTTTTCAAGAATTTTTTCGGCCCCCTGCTTTTTCCAc                                                                        >  2:683120/1‑93 (MQ=255)

                  gCTGAAGCCAATCCAAAGCTGATCACCTTCTGGATCGCGGCGAACGATATGCACGTTTTCATGAATTTTTTCGGCCCCCTGCTTTTTCCACGt                                                                      <  2:2135766/93‑1 (MQ=255)

                   cTGAAGCCAATCCAAATCTGATCACCTTCTGGATCGCGGCGAACGATATGCACGTTTTCAAGAATTATTTCGGCCCCCTGCTTTTTCCACGta                                                                     >  1:6069346/1‑93 (MQ=255)

                   cTGAAGCCAATCCAAAGCTGATCACCTTCTGGATCGCGGCGAACGATATGCACGTTTTCAAGAATTTTTTCGGCCCCCTGCTTTTTCCACGta                                                                     <  2:2585077/93‑1 (MQ=255)

                     taaGCCAATCCAAAGCTGATCACCTTCTGGATCGCGGCGAACGATATGCACGTTTTCAAGAATTTTTTCGGCCCCCTGCTTTTTCCACGtata                                                                   <  2:827291/92‑1 (MQ=255)

                     gAAGCCAATCCAAAGCTGATCACCTTCTGGATCGCGGCGAACGATATGCACGTTTTCAAGAATTTTTTCGGCCCCCTGCTTTTTCCACGtata                                                                   <  1:5004085/93‑1 (MQ=255)

                     gAAGCCAATCCAAAGCTGATCACCTTCTGGATCGCGGCGAACGATATGCACGTTTTCAAGAATTTTTTCGGCCCCCTGCTTTTTCCACGtata                                                                   >  2:5771336/1‑93 (MQ=255)

                     gAAGCCAATCCAAAGCTGATCACCTTCTGGATCGCGGCGAACGATATGCACGTTTTCAAGAATTTTTTCGGCCCCCTGCTTTTTCCACGtata                                                                   <  2:5063657/93‑1 (MQ=255)

                     gAAGCCAATCCAAAGCTGATCACCTTCTGGATCGCGGCGAACGATATGCACGTTTTCAAGAATTTTTTCGGCCCCCTGCTTTTTCCACGtata                                                                   >  1:1020337/1‑93 (MQ=255)

                      aaGCCAATCCAAAGCTGATCACCTTCTGGATCGCGGCGAACGATATGCACGTTTTCAAGAATTTTTTCGGCCCCCTGCTTTTTCCACGTATAg                                                                  >  2:3693285/1‑93 (MQ=255)

                       aGCCAATCCAAAGCTGATCACCTTCTGGATCGCGGCGAACGATATGCACGTTTTCAAGAATTTTTTCGGCCCCCTGCTTTTTCCACGTATAGc                                                                 <  1:851423/93‑1 (MQ=255)

                       aGCCAATCCAAAGCTGATCACCTTCTGGATCGCGGCGAACGATATGCACGTTTTCAAGAATTTTTTCGGCCCCCTGCTTTTTCCACGTATAGc                                                                 >  1:4783740/1‑93 (MQ=255)

                       aGCCAATCCAAAGCTGATCACCTTCTGGATCGCGGCGAACGATATGCACGTTTTCAAGAATTTTTTCGGCCCCCTGCTTTTTCCACGTATAGc                                                                 <  1:4206516/93‑1 (MQ=255)

                        gCCAATCCAAAGCTGATCACCTTCTGGATCGCGGCGAACGATATGCACGTTTTCAAGAATTTTTTCGGCCCCCTGCTTTTTCCACGTATAGCa                                                                >  2:4332243/1‑93 (MQ=255)

                        gCCAATCCAAAGCTGATCACCTTCTGGATCGCGGCGAACGATATGCACGTTTTCAAGAATTTTTTCGGCCCCCTGCTTTTTCCACGTATAGCa                                                                >  2:4139482/1‑93 (MQ=255)

                        gCCAATCCAAAGCTGATCACCTTCTGGATCGCGGCGAACGATATGCACGTTTTCAAGAATTTTTTCGGCCCCCTGCTTTTTCCACGTATAGCa                                                                <  2:4382548/93‑1 (MQ=255)

                         ccAATCCAAAGCTGATCACCTTCTGGATCGCGGCGAACGATATGCACGTTTTCAAGAATTTTTTCGGCCCCCTGCTTTTTCCACGTATAGCAt                                                               >  1:5249482/1‑93 (MQ=255)

                          cAATCCAAAGCTGATCACCTTCTGGATCGCGGCGAACGATATGCACGTTTTCAAGAATTTTTTCGGCCCCCTGCTTTTTCCACGTATAGCATg                                                              >  1:1466871/1‑93 (MQ=255)

                           aaTCCAAAGCTGATCACCTTCTGGATCGCGGCGAACGATATGCACGTTTTCAAGAATTTTTTCGGCCCCCTGCTTTTTCCACGTATAGCATGc                                                             >  1:2763348/1‑93 (MQ=255)

                           aaTCCAAAGCTGATCACCTTCTGGATCGCGGCGAACGATATGCACGTTTTCAAGAATTTTTTCGGCCCCCTGCTTTTTCCACGTATAGCATGc                                                             >  2:5430856/1‑93 (MQ=255)

                            aTCCAAAGCTGATCACCTTCTGGATCGCGGCGAACGATATGCACGTTTTCAAGAATTTTTTCGGCCCCCTGCTTTTTCCACGTATAGCATGCa                                                            >  2:2959280/1‑93 (MQ=255)

                              ccAAAGCTGATCACCTTCTGGATCGCGGCGAACGATATGCACGTTTTCAAGAATTTTTTCGGCCCCCTGCTTTTTCCACGTATAGCATGCAAg                                                          <  2:3214753/93‑1 (MQ=255)

                               cAAAGCTGATCACCTTCTGGATCGCGGCGAACGATATGCACGTTTTCAAGAATTTTTTCGGCCCCCTGCTTTTTCCACGTATAGCATGCAAgg                                                         >  2:2554701/1‑93 (MQ=255)

                               cAAAGCTGATCACCTTCTGGATCGCGGCGAACGATATGCACGTTTTCAAGAATTTTTTCGGCCCCCTGCTTTTTCCACGTATAGCATGCAAgg                                                         <  1:128574/93‑1 (MQ=255)

                               cAAAGCTGATCACCTTCTGGATCGCGGCGAACGATATGCACGTTTTCAAGAATTTTTTCGGCCCCCTGCTTTTTCCACGTATAGCATGCAAgg                                                         >  2:3811236/1‑93 (MQ=255)

                               cAAAGCTGATCACCTTCTGGATCGCGGCGAACGATATGCACGCTTTCAAGAATTTTTTCGGCCCCCTGCTTTTTCCACGTATAGCATGCAAgg                                                         >  1:4550597/1‑93 (MQ=255)

                                aaaGCTGATCACCTTCTGGATCGCGGCGAACGATATGCACGTTTTCAAGAATTTTTTCGGCCCCCTGCTTTTTCCACGTATAGCATGCAAGGa                                                        <  1:4290762/93‑1 (MQ=255)

                                 aaGCTGATCACCTTCTGGATCGCGGCGAACGATATGCACGTTTTCAAGAATTTTTTCGGCCCCCTGCTTTTTCCACGTATAGCATGCAAGGaa                                                       <  2:115112/93‑1 (MQ=255)

                                    cTGATCACCTTCTGGATCGCGGCGAACGATATGCACGTTTTCAAGAATTTTTTCGGCCCCCTGCTTTTTCCACGTATAGCATGCAAGGAATAg                                                    <  1:783850/93‑1 (MQ=255)

                                    cTGATCACCTTCTGGATCGCGGCGAACGATATGCACGTTTTCAAGAATTTTTTCGGCCCCCTGCTTTTTCCACGTATAGCATGCAAGGAATAg                                                    <  2:4473159/93‑1 (MQ=255)

                                      gATCACCTTCTGGATCGCGGCGAACGATATGCACGTTTTCAAGAATTTTTTCGGCCCCCTGCTTTTTCCACGTATAGCATGCAAGGAATAggc                                                  >  1:3165029/1‑93 (MQ=255)

                                      gATCACCTTCTGGATCGCGGCGAACGATATGCACGTTTTCAAGAATTTTTTCGGCCCCCTGCTTTTTCCACGTATAGCATGCAAGGAATAggc                                                  >  2:5940329/1‑93 (MQ=255)

                                      gATCACCTTCTGGATCGCGGCGAACGATATGCACGTTTTCAAGAATTTTTTCGGCCCCCTGCTTTTTCCACGTATAGCATGCAAGGAATAggc                                                  >  1:2124227/1‑93 (MQ=255)

                                      gATCACCTTCTGGATCGCGGCGAACGATATGCACGTTTTCAAGAATTTTTTCGGCCCCCTGCTTTTTCCACGTATAGCATGCAAGGAATAggc                                                  <  2:4791074/93‑1 (MQ=255)

                                      gATCACCTTCTGGATCGCGGCGAACGATATGCACGTTTTCAAGAATTTTTTCGGCCCCCTGCTTTTTCCACGTATAGCATGCAAGGAATAggc                                                  <  2:2017918/93‑1 (MQ=255)

                                       aTCACCTTCTGGATCGCGGCGAACGATATGCACGTTTTCAAGAATTTTTTCGGCCCCCTGCTTTTTCCACGTATAGCATGCAAGGAATAggct                                                 >  2:6270135/1‑93 (MQ=255)

                                       aTCACCTTCTGGATCGCGGCGAACGATATGCACGTTTTCAAGAATTTTTTCGGCCCCCTGCTTTTTCCACGTATAGCATGCAAGGAATAggct                                                 <  2:777924/93‑1 (MQ=255)

                                        tCACCTTCTGGATCGCGGCGAACGATATGCACGTTTTCATGAATTTTTTCGGCCCCCTGCTTTTTCCACGTATAGCATGCAAGGAATAggctg                                                >  2:340975/1‑93 (MQ=255)

                                        tCACCTTCTGGATCGCGGCGAACGATATGCACGTTTTCAAGAATTTTTTCGGCCCCCTGCTTTTTCCACGTATAGCATGCAAGGAATAggctg                                                <  1:1045203/93‑1 (MQ=255)

                                        tCACCTTCTGGATCGCGGCGAACGATATGCACGTTTTCAAGAATTTTTTCGGCCCCCTGCTTTTTCCACGTATAGCATGCAAGGAATAggctg                                                <  2:1089262/93‑1 (MQ=255)

                                        tCACCTTCTGGATCGCGGCGAACGATATGCACGTTTTCAAGAATTTTTTCGGCCCCCTGCTTTTTCCACGTATAGCATGCAAGGAATAggctg                                                <  2:1357146/93‑1 (MQ=255)

                                         cACCTTCTGGATCGCGGCGAACGATATGCACGTTTTCAAGAATTTTTTCGGCCCCCTGCTTTTTCCACGTATAGCATGCAAGGAATAggctgg                                               <  2:4706899/93‑1 (MQ=255)

                                           ccTTCTGGATCGCGGCGAACGATATGCACGTTTTCAAGAATTTTTTCGGCCCCCTGCTTTTTCCACGTATAGCATGCAAGGAATAggctggct                                             <  2:1885284/93‑1 (MQ=255)

                                            cTTCTGGATCGCGGCGAACGATATGCACGTTTTCAAGAATTTTTTCGGCCCCCTGCTTTTTCCACGTATAGCATGCAAGGAATAGGCTGGCtt                                            >  1:1186025/1‑93 (MQ=255)

                                               cTGGATCGCGGCGAACGATATGCACGTTTTCAAGAATTTTTTCGGCCCCCTGCTTTTTCCACGTATAGCATGCAAGGAATAGGCTGGCTTTCg                                         >  2:1272040/1‑93 (MQ=255)

                                               cTGGATCGCGGCGAACGATATGCACGTTTTCAAGAATTTTTTCGGCCCCCTGCTTTTTCCACGTATAGCATGCAAGGAATAGGCTGGCTTTCg                                         >  2:285940/1‑93 (MQ=255)

                                                 ggATCGCGGCGAACGATATGCACGTTTTCAAGAATTTTTTCGGCCCCCTGCTTTTTCCACGTATAGCATGCAAGGAATAGGCTGGCTTTCgtg                                       >  2:3584108/1‑93 (MQ=255)

                                                  gATCGCGGCGAACGATATGCACGTTTTCAAGAATTTTTTCGGCCCCCTGCTTTTTCCACGTATAGCATGCAAGGAATAGGCTGGCTTTCgtgg                                      <  2:1767621/93‑1 (MQ=255)

                                                     cgcgGCGAACGATATGCACGTTTTCAAGAATTTTTTCGGCCCCCTGCTTTTTCCACGTATAGCATGCAAGGAATAGGCTGGCTTTCGTGGtgc                                   <  2:556222/93‑1 (MQ=255)

                                                      gcggcgAACGATATGCACGTTTTCAAGAATTTTTTCGGCCCCCTGCTTTTTCCACGTATAGCATGCAAGGAATAGGCTGGCTTTCGTGGtgct                                  >  1:5184952/1‑93 (MQ=255)

                                                         gcgAACGATATGCACGTTTTCAAGAATTTTTTCGGCCCCCTGCTTTTTCCACGTATAGCATGCAAGGAATAGGCTGGCTTTCGTGGTGCTGcc                               >  1:383620/1‑93 (MQ=255)

                                                           gAACGATATGCACGTTTTCAAGAATTTTTTCGGCCCCCTGCTTTTTCCACGTATAGCATGCAAGGAATAGGCTGGCTTTCGTGGTGCTGCCgg                             >  1:5112391/1‑93 (MQ=255)

                                                            aaCGATATGCACGTTTTCAAGAATTTTTTCGG‑CCCCTGCTTTTTCCACGTATAGCATGCAAGGAATAGGCTGGCTTTCGTGGTGCTGCCGGAt                           <  1:1452137/93‑1 (MQ=255)

                                                            aaCGATATGAACGTTTTCAAGAATTTTTTCGGCCCCCTGCTTTTTCCACGTATAGCATGCAAGGAATAGGCTGGCTTTCGTGGTGCTGCCGGa                            <  1:5456727/93‑1 (MQ=255)

                                                             aCGATATGCACGTTTTCAAGAATTTTTTCGGCCCCCTGCTTTTTCCACGTATAGCATGCAAGGAATAGGCTGGCTTTCGTGGTGCTGCCGGAt                           <  1:930105/93‑1 (MQ=255)

                                                              cGATATGCACGTTTTCAAGAATTTTTTCGGCCCCCTGCTTTTTCCACGTATAGCATGCAAGGAATAGGCTGGCTTTCGTGGTGCTGCCGGATc                          >  1:3738740/1‑93 (MQ=255)

                                                                atatGCACGTTTTCAAGAATTTTTTCGGCCCCCTGCTTTTTCCACGTATAGCATGCAAGGAATAGGCTGGCTTTCGTGGTGCTGCCGGATCGt                        >  2:4577217/1‑93 (MQ=255)

                                                                   tGCACGTTTTCAAGAATTTTTTCGGCCCCCTGCTTTTTCCACGTATAGCATGCAAGGAATAGGCTGGCTTTCGTGGTGCTGCCGGATCGTTGc                     <  2:6157343/93‑1 (MQ=255)

                                                                     cACGTTTTCAAGAATTTTTTCGGCCCCCTGCTTTTTCCACGTATAGCATGCAAGGAATAGGCTGGCTTTCGTGGTTCTGCCGGATCGTTGCGt                   >  1:5549226/1‑93 (MQ=255)

                                                                      aCGTTTTCAAGAATTTTTTCGGCCCCCTGCTTTTTCCACGTATAGCATGCAAGGAATAGGCTGGCTTTCGTGGTGCTGCCGGATCGTTGCGTg                  >  1:4684834/1‑93 (MQ=255)

                                                                       cGTTTTCAAGAATTTTTTCGGCCCCCTGCTTTTTCCACGTATAGCATGCAAGGAATAGGCTGGCTTTCGTGGTGCTGCCGGATCGTTGCGTGc                 >  1:3068518/1‑93 (MQ=255)

                                                                          tttCAAGAATTTTTTCGGCCCCCTGCTTTTTCCACGTATAGCATGCAAGGAATAGGCTGGCTTTCGTGGTGCTGCCGGATCGTTGCGTGCAAc              >  1:5300039/1‑93 (MQ=255)

                                                                          tttCAAGAATTTTTTCGGCCCCCTGCTTTTTCCACGTATAGCATGCAAGGAATAGGCTGGCTTTCGTGGTGCTGCCGGATCGTTGCGTGCAAc              <  1:4483075/93‑1 (MQ=255)

                                                                           ttCAAGAATTTTTTCGGCCCCCTGCTTTTTCCACGTATAGCATGCAAGGAATAGGCTGGCTTTCGTGGTGCTGCCGGATCGTTGCGTGCAACa             <  1:111650/93‑1 (MQ=255)

                                                                           ttCAAGAATTTTTTCGGCCCCCTGCTTTTTCCACGTATAGCATGCAAGGAATAGGCTGGCTTTCGTGGTGCTGCCGGATCGTTGCGTGCAACa             <  2:5694631/93‑1 (MQ=255)

                                                                           ttCAAGAATTTTTTCGGCCCCCTGCTTTTTCCACGTATAGCATGCAAGGAATAGGCTGGCTTTCGTGGTGCTGCCGGATCGTTGCGTGCAACa             <  1:2190596/93‑1 (MQ=255)

                                                                            tCAAGAATTTTTTCGG‑CCCCTGCTTTTTCCACGTATAGCATGCAAGGAATAGGCTGGCTTTCGTGGTGCTGCCGGATCGTTGCGTGCAACAGc           <  1:230281/93‑1 (MQ=255)

                                                                             cAAGAATTTTTTCGGCCCCCTGCTTTTTCCACGTATAGCATGCAAGGAATAGGCTGGCTTTCGTGGTGCTGCCGGATCGTTGCGTGCAACAGc           >  2:5965628/1‑93 (MQ=255)

                                                                               aGAATTTTTTCGGCCCCCTGCTTTTTCCACGTATAGCATGCAAGGAATAGGCTGGCTTTCGTGGTGCTGCCGGATCGTTGCGTGCAACAGGAc         >  2:4018077/1‑93 (MQ=255)

                                                                               aGAATTTTTTCGGCCCCCTGCTTTTTCCACGTATAGCATGCAAGGAATAGGCTGGCTTTCGTGGTGCTGCCGGATCGTTGCGTGCAACAGCAc         <  2:2496110/93‑1 (MQ=255)

                                                                               aGAATTTTTTCGGCCCCCTGCTTTTTCCACGTATAGCATGCAAGGAATAGGCTGGCTTTCGTGGTGCTGCCGGATCGTTGCGTGCAACAGCAc         <  2:1451449/93‑1 (MQ=255)

                                                                               aGAATTTTTTCGG‑CCCCTGCTTTTTCCACGTATAGCACGCAAGGAATAGGCTGGCTTTCGTGGTGCTGCCGGATCGTTGCGTGCAACAGCACt        >  1:3359912/1‑93 (MQ=255)

                                                                                gAATTTTTTCGGCCCCCTGCTTTTTCCACGTATAGCCTGCAAGGAATAGGCTGGCTTTCGTGGTGCTGCCGGATCGTTACGTGCAAATGCACt        >  1:3035227/1‑93 (MQ=255)

                                                                                gAATTTTTTCGGCCCCCTGCTTTTTCCACGTATAGCATGCAAGGAATAGGCTGGCTTTCGTGGTGCTGCCGGATCGTTGCGTGCAACAGCACt        <  2:1528302/93‑1 (MQ=255)

                                                                                  aTTTTTTCGGCCCCCTGCTTTTTCCACGTATAGCATGCAAGGAATAGGCTGGCTTTCGTGGTGCTGCCGGATCGTTGCGTGCAACAGCACTCg      >  1:1762085/1‑93 (MQ=255)

                                                                                   ttttttCGGCCCCCTGCTTTTTCCACGTATAGCATGCAAGGAATAGGCTGGCTTTCGTGGTGCTGCCGGATCGTTGCGTGCAACAGCACTCGa     >  1:485226/1‑93 (MQ=255)

                                                                                      tttCGGCCCCCTGCTTTTTCCACGTATAGCATGCAAGGAATAGGCTGGCTTTCGTGGTGCTGCCGGATCGTTGCGTGCAACAGCACTCGAccc  <  2:4608366/93‑1 (MQ=255)

AATATCAGTCACCAACTCGCTGAAGCCAATCCAAAGCTGATCACCTTCTGGATCGCGGCGAACGATATGCACGTTTTCAAGAATTTTTTCGG‑CCCCTGCTTTTTCCACGTATAGCATGCAAGGAATAGGCTGGCTTTCGTGGTGCTGCCGGATCGTTGCGTGCAACAGCACTCGACCC  >  NC_000913/2761966‑2762143

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 24 ≤` `ATCG/ATCG` `< 30 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

BRESEQ :: bam2aln output

ATCAGTCACCAACTCGCTGAAGCCAATCCAAAGCTGATCACCTTCTGGATCGCGGCGAACGATATGCACGTTTTCAAGAATTTTTTCG‑G‑CCCCTGCTTTTTCCACGTATAGCATGCAAGGAATAGGCTGGCTTTCGTGGTGCTGCCGGATCGTTGCGTGCAACAGCACTCGACCCAAAAATTCGCT  >  NC_000913/2761969‑2762154

ATCAGTCACCAACTCGCTGAAGCCAATCCAAAGCTGATCACCTTCTGGATCGCGGCGAACGATATGCACGTTTTCAAGAATTTTTTCG‑G‑CCCCC                                                                                              >  SRR4302537.988708/1‑94 (MQ=60)

ATCAGTCACCAACTCGCTGAAGCCAATCCAAAGCTGATCACCTTCTGGATCGCGGCGAACGATATGCACGTTTTCAAGAATTTTTTCG‑G‑CCCCC                                                                                              >  SRR4302537.6111558/1‑94 (MQ=60)

ATCAGTCACCAACTCGCTGAAGCCAATCCAAAGCTGATCACCTTCTGGATCGCGGCGAACGATATGCACGTTTTCAAGAATTTTTTCG‑G‑CCCCC                                                                                              >  SRR4302537.3624163/1‑94 (MQ=60)

 TCAGTCACCAACTCGCTGAAGCCAATCCAAAGCTGATCACCTTCTGGATCGCGGCGAACGATATGCACGTTTTCAAGAATTTTTTCG‑G‑CCCCct                                                                                             <  SRR4302537.4603434/94‑3 (MQ=60)

 TCAGTCACCAACTCGCTGAAGCCAATCCAAAGCTGATCACCTTCTGGATCGCGGCGAACGATATGCACGTTTTCAAGAATTTTTTCG‑G‑CCCCct                                                                                             <  SRR4302537.1934661/94‑3 (MQ=60)

 TCAGTCACCAACTCGCTGAAGCCAATCCAAAGCTGATCACCTTCTGGATCGCGGCGAACGATATGCACGTTTTCAAGAATTTTTTCG‑G‑CCCCct                                                                                             >  SRR4302537.2295208/1‑92 (MQ=60)

  CAGTCACCAACTCGCTGAAGCCAATCCAAAGCTGATCACCTTCTGGATCGCGGCGAACGATATGCACGTTTTCAAGAATTTTTTCG‑G‑CCCCctg                                                                                            <  SRR4302537.1166919/94‑4 (MQ=60)

  CAGTCACCAACTCGCTGAAGCCAATCCAAAGCTGATCACCTTCTGGATCGCGGCGAACGATATGCACGTTTTCAAGAATTTTTTCG‑G‑CCCCctg                                                                                            <  SRR4302537.3485741/94‑4 (MQ=60)

  CAGTCACCAACTCGCTGAAGCCAATCCAAAGCTGATCACCTTCTGGATCGCGGCGAACGATATGCACGTTTTCAAGAATTTTTTCG‑G‑CCCCctg                                                                                            <  SRR4302537.5692054/94‑4 (MQ=60)

   AGTCACCAACTCGCTGAAGCCAATCCAAAGCTGATCACCTTCTGGATCGCGGCGAACGATATGCACGTTTTCAAGAATTTTTTCG‑GCCCCCTGC                                                                                            <  SRR4302537.5918035/94‑1 (MQ=60)

    GTCACCAACTCGCTGAAGCCAATCCAAAGCTGATCACCTTCTGGATCGCGGCGAACGATATGCACGTTTTCAAGAATTTTTTCG‑GCCCCCTGCT                                                                                           >  SRR4302537.4309902/1‑94 (MQ=60)

    GTCACCAACTCGCTGAAGCCAATCCAAAGCTGATCACCTTCTGGATCGCGGCGAACGATATGCACGTTTTCAAGAATTTTTTCG‑GCCCCCTGCT                                                                                           >  SRR4302537.1163836/1‑94 (MQ=60)

    GTCACCAACTCGCTGAAGCCAATCCAAAGCTGATCACCTTCTGGATCGCGGCGAACGATATGCACGTTTTCAAGAATTTTTTCG‑GCCCCCTGCT                                                                                           <  SRR4302537.4133442/94‑1 (MQ=60)

       ACCAACTCGCTGAAGCCAATCCAAAGCTGATCACCTTCTGGATCGCGGCGAACGATATGCACGTTTTCAAGAATTTTTTCG‑GCCCCCTGCTTTT                                                                                        <  SRR4302537.3505062/94‑1 (MQ=60)

       ACCAACTCGCTGAAGCCAATCCAAAGCTGATCACCTTCTGGATCGCGGCGAACGATATGCACGTTTTCAAGAATTTTTTCG‑GCCCCCTGCTTTT                                                                                        <  SRR4302537.2593913/94‑1 (MQ=60)

        CCAACTCGCTGAAGCCAATCCAAAGCTGATCACCTTCTGGATCGCGGCGAACGATATGCACGTTTTCAAGAATTTTTTCG‑GCCCCCTGCTTTTT                                                                                       <  SRR4302537.680877/94‑1 (MQ=60)

        CCAACTCGCTGAAGCCAATCCAAAGCTGATCACCTTCTGGATCGCGGCGAACGATATGCACGTTTTCAAGAATTTTTTCG‑GCCCCCTGCTTTTT                                                                                       <  SRR4302537.1053553/94‑1 (MQ=60)

        CCAACTCGCTGAAGCCAATCCAAAGCTGATCACCTTCTGGATCGCGGCGAACGATATGCACGTTTTCAAGAATTTTCTCG‑GCCCCCTGCTTTTT                                                                                       >  SRR4302537.4243784/1‑94 (MQ=60)

         CAACTCGCTGAAGCCAATCCAAAGCTGATCACCTTCTGGATCGCGGCGAACGATATGCACGTTTTCAAGAATTTTTTCG‑GCCCCCTGCTTTTTC                                                                                      >  SRR4302537.620614/1‑94 (MQ=60)

          AACTCGCTGAAGCCAATCCAAAGCTGATCACCTTCTGGATCGCGGCGAACGATATGCACGTTTTCAAGAATTTTTTCG‑GCCCCCTGCTTTTTCC                                                                                     >  SRR4302537.2474998/1‑94 (MQ=60)

          AACTCGCTGAAGCCAATCCAAAGCTGATCACCTTCTGGATCGCGGCGAACGATATGCACGTTTTCAAGAATTTTTTCG‑GCCCCCTGCTTTTTCC                                                                                     >  SRR4302537.979145/1‑94 (MQ=60)

            CTCGCTGAAGCCAATCCAAAGCTGATCACCTTCTGGATCGCGGCGAACGATATGCACGTTTTCAAGAATTTTTTCG‑GCCCCCTGCTTTTTCCAC                                                                                   >  SRR4302537.2748296/1‑94 (MQ=60)

            CTCGCTGAAGCCAATCCAAAGCTGATCACCTTCTGGATCGCGGCGAACGATATGCACGTTTTCAAGAATTTTTTCG‑GCCCCCTGCTTTTTCCAC                                                                                   >  SRR4302537.6461815/1‑94 (MQ=60)

            CTCGCTGAAGCCAATCCAAAGCTGATCACCTTCTGGATCGCGGCGAACGATATGCACGTTTTCAAGAATTTTTTCG‑GCCCCCTGCTTTTTCCAC                                                                                   <  SRR4302537.1060151/94‑1 (MQ=60)

             TCGCTGAAGCCAATCCAAAGCTGATCCCCTTCTGGAGCGCGGCGAACGATATGCACGTTTTCAAGAATTTTTTCG‑GCCCCCTGCTTTTTCCACG                                                                                  <  SRR4302537.1702057/94‑1 (MQ=60)

              CGCTGAAGCCAATCCAAAGCTGATCACCTTCTGGATCGCGGCGAACGATATGCACGTTTTCAAGAATTTTTTCG‑GCCCCCTGCTTTTTCCACGT                                                                                 >  SRR4302537.2014548/1‑94 (MQ=60)

               GCTGAAGCCAATCCAAATCTGATCACCTTCTGGATCGCGGCGAACGATATGCACGTTTTCAAGAATTATTTCG‑GCCCCCTGCTTTTTCCACGTA                                                                                >  SRR4302537.6111046/1‑94 (MQ=60)

               GCTGAAGCCAATCCAAAGCTGATCACCTTCTGGATCGCGGCGAACGATATGCACGTTTTCAAGAATTTTTTCG‑GCCCCCTGCTTTTTCCACGTA                                                                                <  SRR4302537.1319569/94‑1 (MQ=60)

                CTGAAGCCAATCCAAAGCTGATCACCTTCTGGATTTCGGCGAACGATATGCACGTGTTCAAGAATTTTTTCGCC‑CCCCTGATTTTTCCACGTAT                                                                               >  SRR4302537.2872334/1‑94 (MQ=60)

                CTGAAGCCAATCCAAAGCTGATCACCTTCTGGATCGCGGCGAACGATATGCACGTTTTCAAGAATTTTTTCG‑GCCCCCTGCTTTTTCCACGTAT                                                                               <  SRR4302537.5330043/94‑1 (MQ=60)

                CTGAAGCCAATCCAAAGCTGATCACCTTCTGGATCGCGGCGAACGATATGCACGTTTTCAAGAATTTTTTCG‑GCCCCCTGCTTTTTCCACGTAT                                                                               >  SRR4302537.5149092/1‑94 (MQ=60)

                 TGAAGCCAATCCAAAGCTGATCACCTTCTGGATCGCGGCGAACGATATGCACGTTTTCAAGAATTTTTTCG‑GCCCCCTGCTTTTTCCACGTATA                                                                              >  SRR4302537.1027369/1‑94 (MQ=60)

                 TGAAGCCAATCCAAAGCTGATCACCTTCTGGATCGCGGCGAACGATATGCACGTTTTCAAGAATTTTTTCG‑GCCCCCTGCTTTTTCCACGTATA                                                                              <  SRR4302537.2748360/94‑1 (MQ=60)

                  GAAGCCAATCCAAAGCTGATCACCTTCTGGGTCGCGGCGAACGATATGCGCGTTTTCAAGAATTTTTTCG‑GCCCCCTGCTTTTTCCACGTATAG                                                                             <  SRR4302537.1743304/94‑1 (MQ=60)

                  GAAGCCAATCCAAAGCTGATCACCTTCTGGATCGCGGCGAACGATATGCACGTTTTCAAGAATTTTTTCG‑GCCCCCTGCTTTTTCCACGTATAG                                                                             <  SRR4302537.5038551/94‑1 (MQ=60)

                   AAGCCAATCCAAAGCTGATCACCTTCTGGATCGCGGCGAACGATATGCACGTTTTCAAGAATTTTTTCG‑GCCCCCTGCTTTTTCCACGTATAGC                                                                            >  SRR4302537.4816759/1‑94 (MQ=60)

                    AGCCAATCCAAAGCTGATCACCTTCTGGATCGCGGCGAACGATATGCACGTTTTCAAGAATTTTTTCG‑GCCCCCTGCTTTTTCCACGTATAGCA                                                                           <  SRR4302537.4235870/94‑1 (MQ=60)

                    AGCCAATCCAAAGCTGATCACCTTCTGGATCGCGGCGAACGATATGCACGTTTTCAAGAATTTTTTCG‑GCCCCCTGCTTTTTCCACGTATAGCA                                                                           <  SRR4302537.857258/94‑1 (MQ=60)

                    AGCCAATCCAAAGCTGATCACCTTCTGGATCGCGGCGAACGATATGCACGTTTTCAAGAATTTTTTCG‑GCCCCCTGCTTTTTCCACGTATAGCA                                                                           <  SRR4302537.2724969/94‑1 (MQ=60)

                     GCCAATCCAAAGCTGATCACCTTCTGGATCGCGGCGAACGATATGCACGTTTTCAAGAATTTTTTCG‑GCCCCCTGCTTTTTCCACGTATAGCAT                                                                          >  SRR4302537.5285607/1‑94 (MQ=60)

                      CCAATCCAAAGCTGATCACCTTCTGGATCGCGGCGAACGATATGCACGTTTTCAAGAATTTTTTCG‑GCCCCCTGCTTTTTCCACGTATAGCATG                                                                         <  SRR4302537.1784068/94‑1 (MQ=60)

                      CCAATCCAAAGCTGATCACCTTCTGGATCGCGGCGAACGATATGCACGTTTTCAAGAATTTTTTCG‑GCCCCCTGCTTTTTCCACGTATAGCATG                                                                         >  SRR4302537.3731794/1‑94 (MQ=60)

                      CCAATCCAAAGCTGATCACCTTCTGGATCGCGGCGAACGATATGCACGTTTTCAAGAATTTTTTCG‑GCCCCCTGCTTTTTCCACGTATAGCATG                                                                         <  SRR4302537.2720240/94‑1 (MQ=60)

                      CCAATCCAAAGCTGATCACCTTCTGGATCGCGGCGAACGATATGCACGTTTTCAAGAATTTTTTCG‑GCCCCCTGCTTTTTCCACGTATAGCATG                                                                         >  SRR4302537.1477071/1‑94 (MQ=60)

                       CAATCCAAAGCTGATCACCTTCTGGATCGCGGCGAACGATATGCACGTTTTCAAGAATTTTTTCG‑GCCCCCTGCTTTTTCCACGTATAGCATGC                                                                        >  SRR4302537.2782627/1‑94 (MQ=60)

                       CAATCCAAAGCTGATCACCTTCTGGATCGCGGCGAACGATATGCACGTTTTCAAGAATTTTTTCG‑GCCCCCTGCTTTTTCCACGTATAGCATGC                                                                        >  SRR4302537.4401376/1‑94 (MQ=60)

                        AATCCAAAGCTGATCACCTTCTGGATCGCGGCGAACGATATGCACGTTTTCAAGAATTTTTTCG‑GCCCCCTGCTTTTTCCACGTATAGCATGCA                                                                       >  SRR4302537.3842908/1‑94 (MQ=60)

                          TCCAAAGCTGATCACCTTCTGGATCGCGGCGAACGATATGCACGTTTTCAAGAATTTTTTCG‑GCCCCCTGCTTTTTCCACGTATAGCATGCAAG                                                                     <  SRR4302537.4838934/94‑1 (MQ=60)

                          TCCAAAGCTGATCACCTTCTGGATCGCGGCGAACGATATGCACGTTTTCAAGAATTTTTTCG‑GCCCCCTGCTTTTTCCACGTATAGCATGCAAG                                                                     <  SRR4302537.5309419/94‑1 (MQ=60)

                           CCAAAGCTGATCACCTTCTGGATCGCGGCGAACGATATGCACGTTTTCAAGAATTTTTTCG‑GCCCCCTGCTTTTTCCACGTATAGCATGCAAGG                                                                    <  SRR4302537.2113287/94‑1 (MQ=60)

                           CCAAAGCTGATCACCTTCTGGATCGCGGCGAACGATATGCACGTTTTCAAGAATTTTTTCG‑GCCCCCTGCTTTTTCCACGTATAGCATGCAAGG                                                                    >  SRR4302537.3192500/1‑94 (MQ=60)

                           CCAAAGCTGATCACCTTCTGGATCGCGGCGAACGATATGCACGCTTTCAAGAATTTTTTCG‑GCCCCCTGCTTTTTCCACGTATAGCATGCAAGG                                                                    >  SRR4302537.4582088/1‑94 (MQ=60)

                            CAAAGCTGATCACCTTCTGGATCGCGGCGAACGATATGCACGTTTTCAAGAATTTTTTCG‑GCCCCCTGCTTTTTCCACGTATAGCATGCAAGGA                                                                   <  SRR4302537.129489/94‑1 (MQ=60)

                             AAAGCTGATCACCTTCTGGATCGCGGCGAACGATATGCACGTTTTCAAGAATTTTTTCG‑GCCCCCTGCTTTTTCCACGTATAGCATGCAAGGAA                                                                  <  SRR4302537.4320583/94‑1 (MQ=60)

                             AAAGCTGATCACCTTCTGGATCGCGGCGAACGATATGCACGTTTTCAAGAATTTTTTCG‑GCCCCCTGCTTTTTCCACGTATAGCATGCAAGGAA                                                                  <  SRR4302537.123440/94‑1 (MQ=60)

                              AAGCTGATCACCTTCTGGATCGCGGCGAACGATATGCACGTTTTCAAGAATTTTTTCG‑GCCCCCTGCTTTTTCCACGTATAGCATGCAAGGAAT                                                                 >  SRR4302537.5432823/1‑94 (MQ=60)

                               AGCTGATCACCTTCTGGATCGCGGCGAACGATATGCACGTTTTCAAGAATTTTTTCG‑GCCCCCTGCTTTTTCCACGTATAGCATGCAAGGAATA                                                                >  SRR4302537.3751262/1‑94 (MQ=60)

                               AGCTGATCACCTTCTGGATCGCGGCGAACGATATGCACGTTTTCAAGAATTTTTTCG‑GCCCCCTGCTTTTTCCACGTATAGCATGCAAGGAATA                                                                >  SRR4302537.5259895/1‑94 (MQ=60)

                                 CTGATCACCTTCTGGATCGCGGCGAACGATATGCACGTTTTCAAGAATTTTTTCG‑GCCCCCTGCTTTTTCCACGTATAGCATGCAAGGAATAGG                                                              <  SRR4302537.789228/94‑1 (MQ=60)

                                 CTGATCACCTTCTGGATCGCGGCGAACGATATGCACGTTTTCAAGAATTTTTTCG‑GCCCCCTGCTTTTTCCACGTATAGCATGCAAGGAATAGG                                                              <  SRR4302537.2484487/94‑1 (MQ=60)

                                  TGATCACCTTCTGGATCGCGGCGAACGATATGCACGTTTTCAAGAATTTTTTCG‑GCCCCCTGCTTTTTCCACGTATAGCATGCAAGGAATAGGC                                                             >  SRR4302537.3325701/1‑94 (MQ=60)

                                  TGATCACCTTCTGGATCGCGGCGAACGATATGCACGTTTTCAAGAATTTTTTCG‑GCCCCCTGCTTTTTCCACGTATAGCATGCAAGGAATAGGC                                                             >  SRR4302537.2139170/1‑94 (MQ=60)

                                  TGATCACCTTCTGGATCGCGGCGAACGATATGCACGTTTTCAAGAATTTTTTCG‑GCCCCCTGCTTTTTCCACGTATAGCATGCAAGGAATAGGC                                                             >  SRR4302537.1673529/1‑94 (MQ=60)

                                  TGATCACCTTCTGGATCGCGGCGAACGATATGCACGTTTTCAAGAATTTTTTCG‑GCCCCCTGCTTTTTCCACGTATAGCATGCAAGGAATAGGC                                                             >  SRR4302537.3187255/1‑94 (MQ=60)

                                     TCACCTTCTGGATCGCGGCGAACGATATGCACGTTTTCAAGAATTTTTTCG‑GCCCCCTGCTTTTTCCACGTATAGCATGCAAGGAATAGGCTGG                                                          <  SRR4302537.1052418/94‑1 (MQ=60)

                                      CACCTTCTGGATCGCGGCGAACGATATGCACGTTTTCAAGAATTTTTTCG‑GCCCCCTGCTTTTTCCACGTATAGCATGCAAGGAATAGGCTGGC                                                         <  SRR4302537.454661/94‑1 (MQ=60)

                                      CACCTTCTGGATCGCGGCGAACGATATGCACGTTTTCAAGAATTTTTTCG‑GCCCCCTGCTTTTTCCACGTATAGCATGCAAGGAATAGGCTGGC                                                         <  SRR4302537.919066/94‑1 (MQ=60)

                                        CCTTCTGGATCGCGGCGAACGATATGCACGTTTTCAAGAATTTTTTCG‑GCCCCCTGCTTTTTCCACGTATAGCATGCAAGGAATAGGCTGGCTT                                                       >  SRR4302537.1194266/1‑94 (MQ=60)

                                         CTTCTGGATCGCGGCGAACGATATGCACGTTTTCAAGAATTTTTTCG‑GCCCCCTGCTTTTTCCACGTATAGCATGCAAGGAATAGGCTGGCTTT                                                      >  SRR4302537.3084682/1‑94 (MQ=60)

                                          TTCTGGATCGCGGCGAACGATATGCACGTTTTCAAGAATTTTTTCG‑GCCCCCTGCTTTTTCCACGTATAGCATGCAAGGAATAGGCTGGATTTC                                                     >  SRR4302537.2238017/1‑94 (MQ=60)

                                            CTGGATCGCGGCGAACGATATGCACGTTTTCAAGAATTTTTTCG‑GCCCCCTGCTTTTTCCACGTATAGCATGCAAGGAATAGGCTGGCTTTCGT                                                   <  SRR4302537.1394617/94‑1 (MQ=60)

                                            CTGGATCGCGGCGAACGATATGCACGTTTTCAAGAATTTTTTCG‑GCCCCCTGCTTTTTCCACGTATAGCATGCAAGGAATAGGCTGGCTTTCGT                                                   <  SRR4302537.3990171/94‑1 (MQ=60)

                                             TGGATCGCGGCGAACGATATGCACGTTTTCAAGAATTTTTTCG‑GCCCCCTGCTTTTTCCACGTATAGCATGCAAGGAATAGGCTGGCTTTCGTG                                                  <  SRR4302537.4884655/94‑1 (MQ=60)

                                               GATCGCGGCGAACGATATGCACGTTTTCAAGAATTTTTTCG‑GCCCCCTGCTTTTTCCACGTATAGCATGCAAGGAATAGGCTGGCTTTCGTGGT                                                <  SRR4302537.569812/94‑1 (MQ=60)

                                               GATCGCGGCGAACGATATGCACGTTTTCAAGAATTTTTTCG‑GCCCCCTGCTTTTTCCACGTATAGCATGCAAGGAATAGGCTGGCTTTCGTGGT                                                >  SRR4302537.792141/1‑94 (MQ=60)

                                                ATCGCGGCGAACGATATGCACGTTTTCAAGAATTTTTTCG‑GCCCCCTGCTTTTTCCACGTATAGCATGCAAGGAATAGGCTGGCTTTCGTGGTG                                               >  SRR4302537.2576186/1‑94 (MQ=60)

                                                 TCGCGGCGAACGATATGCACGTTTTCAAGAATTTTTTCG‑GCCCCCTGCTTTTTCCACGTATAGCATGCAAGGAATAGGCTGGCTTTCGTGGTGC                                              <  SRR4302537.5514254/94‑1 (MQ=60)

                                                  CGCGGCGAACGATATGCACGTTTTCAAGAATTTTTTCG‑GCCCCCTGCTTTTTCCACGTATAGCATGCAAGGAATAGGCTGGCTTTCGTGGTGCT                                             <  SRR4302537.505962/94‑1 (MQ=60)

                                                  CGCGGCGAACGATATGCACGTTTTCAAGAATTTTTTCG‑GCCCCCTGCTTTTTCCACGTATAGCATGCAAGGAATAGGCTGGCTTTCGTGGTGCT                                             >  SRR4302537.5220618/1‑94 (MQ=60)

                                                    CGGCGAACGATATGCACGTTTTCAAGAATTTTTTCG‑GCCCCCTGCTTTTTCCACGTATAGCATGCAAGGAATAGGCTGGCTTTCGTGGTGCTGC                                           >  SRR4302537.2457140/1‑94 (MQ=60)

                                                    CGGCGAACGATATGCACGTTTTCAAGAATTTTTTCG‑GCCCCCTGCTTTTTCCACGTATAGCATGCAAGGAATAGGCTGGCTTTCGTGGTGCTGC                                           >  SRR4302537.3125687/1‑94 (MQ=60)

                                                     GGCGAACGATATGCACGTTTTCAAGAATTTTTTCG‑GCCCCCTGCTTTTTCCACGTATAGCATGCAAGGAATAGGCTGGCTTTCGTGGTGCTGCC                                          <  SRR4302537.911050/94‑1 (MQ=60)

                                                     GGCGAACGATATGCACGTTTTCAAGAATTTTTTCG‑GCCCCCTGCTTTTTCCACGTATAGCATGCAAGGAATAGGCTGGCTTTCGTGGTGCTGCC                                          >  SRR4302537.386278/1‑94 (MQ=60)

                                                     GGCGAACGATATGCACGTTTTCAAGAATTTTTTCG‑GCCCCCTGCTTTTTCCACGTATAGCATGCAAGGAATAGGCTGGCTTTCGTGGTGCTGCC                                          <  SRR4302537.1661287/94‑1 (MQ=60)

                                                       CGAACGATATGCACGTTTTCAAGAATTTTTTCG‑GCCCCCTGCTTTTTCCACGTATAGCATGCAAGGAATAGGCTGGCTTTCGTGGTGCTGCCGG                                        <  SRR4302537.5413408/94‑1 (MQ=60)

                                                       CGAACGATATGCACGTTTTCAAGAATTTTTTCG‑GCCCCCTGCTTTTTCCACGTATAGCATGCAAGGAATAGGCTGGCTTTCGTGGTGCTGCCGG                                        >  SRR4302537.5147631/1‑94 (MQ=60)

                                                         AACGATATGCACGTTTTCAAGAATTTTTTCG‑GCCCCCTGCTTTTTCCACGTATAGCATGCAAGGAATAGGCTGGCTTTCGTGGTGCTGCCGGAT                                      >  SRR4302537.3053435/1‑94 (MQ=60)

                                                         AACGATATGCACGTTTTCAAGAATTTTTTCG‑GCCCCCTGCTTTTTCCACGTATAGCATGCAAGGAATAGGCTGGCTTTCGTGGTGCTGCCGGAT                                      <  SRR4302537.123796/94‑1 (MQ=60)

                                                         AACGATATGCACGTTTTCAAGAATTTTTTCG‑GCCCCCTGCTTTTTCCACGTATAGCATGCAAGGAATAGGCTGGCTTTCGTGGTGCTGCCGGAT                                      >  SRR4302537.4194200/1‑94 (MQ=60)

                                                         AACGATATGCACGTTTTCAAGAATTTTTTCG‑G‑CCCCTGCTTTTTCCACGTATAGCATGCAAGGAATAGGCTGGCTTTCGTGGTGCTGCCGGATC                                     <  SRR4302537.1462255/94‑1 (MQ=60)

                                                         AACGATATGAACGTTTTCAAGAATTTTTTCG‑GCCCCCTGCTTTTTCCACGTATAGCATGCAAGGAATAGGCTGGCTTTCGTGGTGCTGCCGGAT                                      <  SRR4302537.5493945/94‑1 (MQ=60)

                                                          ACGATATGCACGTTTTCAAGAATTTTTTCG‑GCCCCCTGCTTTTTCCACGTATAGCATGCAAGGAATAGGCTGGCTTTCGTGGTGCTGCCGGATC                                     <  SRR4302537.936515/94‑1 (MQ=60)

                                                          ACGATATGCACGTTTTCAAGAATTTTTTCG‑GCCCCCTGCTTTTTCCACGTATAGCATGCAAGGAATAGGCTGGCTTTCGTGGTGCTGCCGGATC                                     >  SRR4302537.3764996/1‑94 (MQ=60)

                                                            GATATGCACGTTTTCAAGAATTTTTTCG‑GCCCCCTGCTTTTTCCACGTATAGCATGCAAGGAATAGGCTGGCTTTCGTGGTGCTGCCGGATCGT                                   >  SRR4302537.4733241/1‑94 (MQ=60)

                                                            GATATGCACGTTTTCAAGAATTTTTTCG‑GCCCCCTGCTTTTTCCACGTATAGCATGCAAGGAATAGGCTGGCTTTCGTGGTGCTGCCGGATCGT                                   >  SRR4302537.3181928/1‑94 (MQ=60)

                                                            GATATGCACGTTTTCAAGAATTTTTTCG‑GCCCCCTGCTTTTTCCACGTATAGCATGCAAGGAATAGGCTGGCTTTCGTGGTGCTGCCGGATCGT                                   >  SRR4302537.3499041/1‑94 (MQ=60)

                                                            GATATGCACGTTTTCAAGAATTTTTTCG‑GCCCCCTGCTTTTTCCACGTATAGCATGCAAGGAATAGGCTGGCTTTCGTGGTGCTGCCGGATCGT                                   >  SRR4302537.447332/1‑94 (MQ=60)

                                                             ATATGCACGTTTTCAAGAATTTTTTCG‑GCCCCCTGCTTTTTCCACGTATAGCATGCAAGGAATAGGCTGGCTTTCGTGGTGCTGCCGGATCGTT                                  >  SRR4302537.2140327/1‑94 (MQ=60)

                                                              TATGCACGTTTTCAAGAATTTTTTCG‑GCCCCCTGCTTTTTCCACGTATAGCATGCAAGGAATAGGCTGGCTTTCGTGGTGCTGCCGGATCGTTG                                 >  SRR4302537.5166842/1‑94 (MQ=60)

                                                               ATGCACGTTTTCAAGAATTTTTTCG‑GCCCCCTGCTTTTTCCACGTATAGCATGCAAGGAATAGGCTGGCTTTCGTGGTGCTGCCGGATCGTTGC                                >  SRR4302537.3473192/1‑94 (MQ=60)

                                                               ATGCACGTTTTCAAGAATTTTTTCG‑GCCCCCTGCTTTTTCCACGTATAGCATGCAAGGAATAGGCTGGCTTTCGTGGTGCTGCCGGATCGTTGC                                >  SRR4302537.6214085/1‑94 (MQ=60)

                                                                 GCACGTTTTCAAGAATTTTTTCG‑GCCCCCTGCTTTTTCCACGTATAGCATGCAAGGAATAGGCTGGCTTTCGTGGTTCTGCCGGATCGTTGCGT                              >  SRR4302537.5587117/1‑94 (MQ=60)

                                                                 GCACGTTTTCAAGAATTTTTTCG‑GCCCCCTGCTTTTTCCACGTATAGCATGCAAGGAATAGGCTGGCTTTCGTGGTGCTGCCGGATTGTTACGT                              >  SRR4302537.4061335/1‑94 (MQ=60)

                                                                  CACGTTTTCAAGAATTTTTTCG‑GCCCCCTGCTTTTTCCACGTATAGCATGCAAGGAATAGGCTGGCTTTCGTGGTGCTGCCGGATCGTTGCGTG                             >  SRR4302537.4717240/1‑94 (MQ=60)

                                                                   ACGTTTTCAAGAATTTTTTCG‑GCCCCCTGCTTTTTCCACGTATAGCATGCAAGGAATAGGCTGGCTTTCGTGGTGCTGCCGGATCGTTGCGTGC                            >  SRR4302537.3089899/1‑94 (MQ=60)

                                                                      TTTTCAAGAATTTTTTCG‑GCCCCCTGCTTTTTCCACGTATAGCATGCAAGGAATAGGCTGGCTTTCGTGGTGCTGCCGGATCGTTGCGTGCAAC                         <  SRR4302537.4999482/94‑1 (MQ=60)

                                                                      TTTTCAAGAATTTTTTCG‑GCCCCCTGCTTTTTCCACGTATAGCATGCAAGGAATAGGCTGGCTTTCGTGGTGCTGCCGGATCGTTGCGTGCAAC                         >  SRR4302537.5336442/1‑94 (MQ=60)

                                                                       TTTCAAGAATTTTTTCG‑GCCCCCTGCTTTTTCCACGTATAGCATGCAAGGAATAGGCTGGCTTTCGTGGTGCTGCCGGATCGTTGCGTGCAACA                        <  SRR4302537.4514087/94‑1 (MQ=60)

                                                                        TTCAAGAATTTTTTCG‑GCCCCCTGCTTTTTCCACGTATAGCATGCAAGGAATAGGCTGGCTTTCGTGGTGCTGCCGGATCGTTGCGTGCAACAG                       >  SRR4302537.821612/1‑94 (MQ=60)

                                                                        TTCAAGAATTTTTTCG‑GCCCCCTGCTTTTTCCACGTATAGCATGCAAGGAATAGGCTGGCTTTCGTGGTGCTGCCGGATCGTTGCGTGCAACAG                       <  SRR4302537.112467/94‑1 (MQ=60)

                                                                        TTCAAGAATTTTTTCG‑GCCCCCTGCTTTTTCCACGTATAGCATGCAAGGAATAGGCTGGCTTTCGTGGTGCTGCCGGATCGTTGCGTGCAACAG                       >  SRR4302537.1051213/1‑94 (MQ=60)

                                                                        TTCAAGAATTTTTTCG‑GCCCCCTGCTTTTTCCACGTATAGCATGCAAGGAATAGGCTGGCTTTCGTGGTGCTGCCGGATCGTTGCGTGCAACAG                       <  SRR4302537.2206028/94‑1 (MQ=60)

                                                                        TTCAAGAATTTTTTCG‑GCCCCCTGCTTTTTCCACGTATAGCATGCAAGGAATAGGCTGGCTTTCGTGGTGCTGCCGGATCGCTGCGTGacacaa                       >  SRR4302537.1200704/1‑88 (MQ=60)

                                                                         TCAAGAATTTTTTCG‑GCCCCCTGCTTTTTCCACGTATAGCATGCAAGGAATAGGCTGGCTTTCGTGGTGCTGCCGGATCGTTGCGTGCAACAGC                      <  SRR4302537.4313533/94‑1 (MQ=60)

                                                                         TCAAGAATTTTTTCG‑G‑CCCCTGCTTTTTCCACGTATAGCATGCAAGGAATAGGCTGGCTTTCGTGGTGCTGCCGGATCGTTGCGTGCAACAGCA                     <  SRR4302537.231909/94‑1 (MQ=60)

                                                                           AAGAATTTTTTCG‑G‑CCCCTGCTTTTTCCACGTATAGCACGCAAGGAATAGGCTGGCTTTCGTGGTGCTGCCGGATCGTTGCGTGCAACAGCACT                   >  SRR4302537.3383711/1‑94 (MQ=60)

                                                                            AGAATTTTTTCG‑GCCCCCTGCTTTTTCCACGTATAGCCTGCAAGGAATAGGCTGGCTTTCGTGGTGCTGCCGGATCGTTACGTGCAAATGCACT                   >  SRR4302537.3056385/1‑94 (MQ=60)

                                                                            AGAATTTTTTCG‑GCCCCCTGCTTTTTCCACGTATAGCATGCAAGGAATAGGCTGGCTTTCGTGGTGCTGCCGGATCGTTGCGTGCAACAGCACT                   >  SRR4302537.5974124/1‑94 (MQ=60)

                                                                            AGAATTTTTTCG‑GCCCCCTGCTTTTTCCACGTATAGCATGCAAGGAATAGGCTGGCTTTCGTGGTGCTGCCGGATCGTTGCGTGCAACAGCACT                   >  SRR4302537.4984778/1‑94 (MQ=60)

                                                                              AATTTTTTCG‑GCCCCCTGCTTTTTCCACGTATAGCATGCAAGGAATAGGCTGGCTTTCGTGGTGCTGCCGGATCGTTGCGTGCAACAGCACTCG                 >  SRR4302537.1774362/1‑94 (MQ=60)

                                                                              AATTTTTTCG‑GCCCCCTGCTTTTTCCACGTATAGCATGCAAGGAATAGGCTGGCTTTCGTGGTGCTGCCGGATCGTTGCGTGCAACAGCACTCG                 >  SRR4302537.1464830/1‑94 (MQ=60)

                                                                               ATTTTTTCG‑GCCCCCTGCTTTTTCCACGTATAGCATGCAAGGAATAGGCTGGCTTTCGTGGTGCTGCCGGATCGTTGCGTGCAACAGCACTCGA                >  SRR4302537.488552/1‑94 (MQ=60)

                                                                                  TTTTCG‑GCCCCCTGCTTTTTCCACGTATAGCATGCAAGGAATAGGCTGGCTTTCGTGGTGCTGCCGGATCGTTGCGTGCAACAGCACTCGACCC             >  SRR4302537.6068067/1‑94 (MQ=60)

                                                                                   TTTCG‑GCCCCCTGCTTTTTCCACGTATAGCATGCAAGGAATAGGCTGGCTTTCGTGGTGCTGCCGGATCGTTGCGTGCAACAGCACTCGACCCA            <  SRR4302537.4608894/94‑1 (MQ=60)

                                                                                     TCG‑GCCCCCTGCTTTTTCCACGTATAGCATGCAAGGAATAGGCTGGCTTTCGTGGTGCTGCCGGATCGTTGCGTGCAACAGCACTCGACCCAAA          >  SRR4302537.6160254/1‑94 (MQ=60)

                                                                                        ggcCCCCTGCTTTTTCCACGTATAGCATGCAAGGAATAGGCTGGCTTTCGTGGTGCTGCCGGATCGTTGCGTGCAACAGCACTCGACCCAAAAA        <  SRR4302537.711258/91‑1 (MQ=60)

                                                                                         C‑CCCCTGCTTTTTCCACGTATAGCATGCAAGGAATAGGCTGGCTTTCGTGGTGCTGCCGGATCGTTGCGTGCAACAGCACTCGACTCAAAAATT      >  SRR4302537.1665/1‑94 (MQ=60)

                                                                                           CCCCTGCTTTTTCCACGTATAGCATGCAAGGAATAGGCTGGCTTTCGTGGTGCTGCCGGATCGTTGCGTGCAACAGCACTCGACCCAAAAATTC     >  SRR4302537.2898186/1‑94 (MQ=60)

                                                                                            CCCTGCTTTTTCCACGTATAGCATGCAAGGAATAGGCTGGCTTTCGTGGTGCTGCCGGATCGTTGCGTGCAACAGCACTCGACCCAAAAATTCG    <  SRR4302537.3373368/94‑1 (MQ=60)

                                                                                             CCTGCTTTTTCCACGTATAGCATGCAAGGAATAGGCTGGCTTTCGTGGTGCTGCCGGATCGTTGCGTGCAACAGCACTCGACCCAAAAATTCGC   >  SRR4302537.5701607/1‑94 (MQ=60)

                                                                                              CTGCTTTTTCCACGTATAGCATGCAAGGAATAGGCTGGCTTTCGTGGTGCTGCCGGATCGTTGCGTGCAACAGCACTCGACCCAAAAATTCGCT  >  SRR4302537.6432372/1‑94 (MQ=60)

                                                                                              CTGCTTTTTCCACGTATAGCATGCAAGGAATAGGCTGGCTTTCGTGGTGCTGCCGGATCGTTGCGTGCAACAGCACTCGACCCAAAAATTCGCT  <  SRR4302537.959200/94‑1 (MQ=60)

ATCAGTCACCAACTCGCTGAAGCCAATCCAAAGCTGATCACCTTCTGGATCGCGGCGAACGATATGCACGTTTTCAAGAATTTTTTCG‑G‑CCCCTGCTTTTTCCACGTATAGCATGCAAGGAATAGGCTGGCTTTCGTGGTGCTGCCGGATCGTTGCGTGCAACAGCACTCGACCCAAAAATTCGCT  >  NC_000913/2761969‑2762154

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 0 ≤` `ATCG/ATCG` `< 8 ≤` `ATCG/ATCG` `< 28 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`