Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I2 R1 882 254.4 12957588 98.0% 12698436 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,766,473 A→G D248G (GAT→GGT)  b2632 → putative GTP‑binding protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,766,4730AG100.0% 592.0 / NA 171D248G (GAT→GGT) b2632putative GTP‑binding protein
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (75/96);  total (75/96)

Only 100 of 172 total aligned reads displayed.

CAGCCCGGTAGTAGCGCTACTGCAACATCCCTTTCGGACATGGGTAGCCCGGGAGCAGGCACGTGACGATTTCGGTGAAACCGTAGATACCATACTCGATACGGTAAGCACCTTTCCCCTTATTCCCGCCCCGGTGCGGACCATTATTCAGGCCGTGCGTTCCTCGGTGGTGTCA  >  NC_000913/2766387‑2766561
                                                                                      |                                                                                        
 agcCCGGTAGTAGCGCTACTGCAACATGCCTTTCGAACAACGGTAGCCCGGGAGCAGGCACGTGACGATTTCGGTGAAACCGTAGGTGCCATa                                                                                   <  1:4324469/93‑1 (MQ=255)
 agcCCGGTAGTAGCGCTACTGCAACATCCCTTTCGAACAACGGTAGCCCGGGAGCAGGCACGTGACGATTTCGGTGAAACCGTAGGTGCCATa                                                                                   <  1:816981/93‑1 (MQ=255)
  gcCCGGTAGTAGCGCTACTGCAACATCCCTTTCGAACAACGGTAGCCCGGGAGCAGGCACGTGACGATTTCGGTGAAACCGTAGGTGCCATAc                                                                                  <  2:4329129/93‑1 (MQ=255)
  gcCCGGTAGTAGCGCTACTGCAACATCCCTTTCGAACAACGGTAGCCCGGGAGCAGGCACGTGACGATTTCGGTGAAACCGTAGGTGCCATAc                                                                                  <  2:4976464/93‑1 (MQ=255)
   cccGGTAGTAGCGCTACTGCAACATCCCTTTCGAACAACGGTAGCCCGGGAGCAGGCACGTGACGATTTCGGTGAAACCGTAGGTGCCATACt                                                                                 >  1:52609/1‑93 (MQ=255)
   cccGGTAGTAGCGCTACTGCAACATCCCTTTCGAACAACGGTAGCCCGGGAGCAGGCACGTGACGATTTCGGTGAAACCGTAGGTGCCATACt                                                                                 >  2:2028932/1‑93 (MQ=255)
    ccGGTAGTAGCGCTACTGCAACATCCCTTTCGAACAACGGTAGCCCGGGAGCAGGCACGTGACGATTTCGGTGAAACCGTAGGTGCCATACTc                                                                                <  2:2268837/93‑1 (MQ=255)
    ccGGTAGTAGCGCTACTGCAACATCCCTTTCGAACAACGGTAGCCCGGGAGCAGGCACGTGACGATTTCGGTGAAACCGTAGGCGCCATACTc                                                                                >  2:1039678/1‑93 (MQ=255)
     cGGTAGTAGCGCTACTGCAACATCCCTTTCGAACAACGGTAGCCCGGGAGCAGGCACGTGACGATTTCGGTGAAACCGTAGGTGCCATACTCg                                                                               <  2:3003326/93‑1 (MQ=255)
      ggTAGTAGCGCTACTGCAACATCCCTTTCGAACAACGGTAGCCCGGGAGCAGGCACGTGACGATTTCGGTGAAACCGTAGGTGCCATACTCGa                                                                              <  2:528045/93‑1 (MQ=255)
      ggTAGTAGCGCTACTGCAACATCCCTTTCGAACAACGGTAGCCCGGGAGCAGGCACGTGACGATTTCGGTGAAACCGTAGGTGCCATACTCGa                                                                              >  1:3664450/1‑93 (MQ=255)
      ggTAGTAGCGCTACTGCAACATCCCTTTCGAACAACGGTAGCCCGGGAGCAGGCACGTGACGATTTCGGTGAAACCGTAGGTGCCATACTCGa                                                                              <  1:6399134/93‑1 (MQ=255)
       gtagtaGCGCTACTGCAACATCCCTTTCGAACAACGGTAGCCCGGGAGCAGGCACGTGACGATTTCGGTGAAACCGTAGGTGCCATACTCGAt                                                                             <  2:3633334/93‑1 (MQ=255)
       gtagtaGCGCTACTGCAACATCCCTTTCGAACAACGGTAGCCCGGGAGCAGGCACGTGACGATTTCGGTGAAACCGTAGGTGCCATACTCGAt                                                                             >  2:2660706/1‑93 (MQ=255)
        tagtagCGCTACTGCAACATCCCTTTCGAACAACGGTAGCCCGGGAGCAGGCACGTGACGATTTCGGTGAAACCGTAGGTGCCATACTCGATa                                                                            >  2:113737/1‑93 (MQ=255)
        tagtagCGCTACTGCAACATCCCTTTCGAACAACGGTAGCCCGGGAGCAGGCACGTGACGATTTCGGTGAAACCGTAGGTGCCATACTCGATa                                                                            >  2:1567965/1‑93 (MQ=255)
        tagtagCGCTACTGCAACATCCCTTTCGAACAACGGTAGCCCGGGAGCAGGCACGTGACGATTTCGGTGAAACCGTAGGTGCCATACTCGATa                                                                            <  2:2684559/93‑1 (MQ=255)
        tagtagCGCTACTGCAACATCCCTTTCGAACAACGGTAGCCCGGGAGCAGGCACGTGACGATTTCGGTGAAACCGTAGGTGCCATACTCGATa                                                                            <  2:6173582/93‑1 (MQ=255)
         agtagCGCTACTGCAACATCCCTTTCGAACAACGGTAGCCCGGGAGCAGGCACGTGACGATTTCGGTGAAACCGTAGGTGCCATACTCGATAc                                                                           >  1:1555864/1‑93 (MQ=255)
            agCGCTACTGCAACATCCCTTTCGAACAACGGTAGCCCGGGAGCAGGCACGTGACGATTTCGGTGAAACCGTAGGTGCCATACTCGATACGGt                                                                        >  2:4709971/1‑93 (MQ=255)
             gcgcTACTGCAACATCCCTTTCGAACAACGGTAGCCCGGGAGCAGGCACGTGACGATTTCGGTGAAACCGTAGGTGCCATACTCGATACGGTa                                                                       <  1:3038262/93‑1 (MQ=255)
              cgcTACTGCAACATCCCTTTCGAACAACGGTAGCCCGGGAGCAGGCACGTGACGATTTCGGTGAAACCGTAGGTGCCATACTCGATACGGTaa                                                                      <  2:2081084/93‑1 (MQ=255)
              cgcTACTGCAACATCCCTTTCGAACAACGGTAGCCCGGGAGCAGGCACGTGACGATTTCGGTGAAACCGTAGGTGCCATACTCGATACGGTaa                                                                      <  2:6109901/93‑1 (MQ=255)
               gcTACTGCAACATCCCTTTCGAACAACGGTAGCCCGGGAGCAGGCACGTGACGATTTCGGTGAAACCGTAGGTGCCATACTCGATACGGTAAg                                                                     <  1:2399645/93‑1 (MQ=255)
               gcTACTGCAACATCCCTTTCGAACAACGGTAGCCCGGGAGCAGGCACGTGACGATTTCGGTGAAACCGTAGGTGCCATACTCGATACGGTAAg                                                                     <  2:5909727/93‑1 (MQ=255)
               gcTACTGCAACATCCCTTTCGAACAACGGTAGCCCGGGAGCAGGCACGTGACGATTTCGGTGAAACCGTAGGTGCCATACTCGAAACGGTAAg                                                                     <  2:6286661/93‑1 (MQ=255)
                cTACTGCAACATCCCTTTCGAACAACGGTAGCCCGGGAGCAGGCACGTGACGATTTCGGTGAAACCGTAGGTGCCATACTCGATACGGTAAGc                                                                    <  2:337589/93‑1 (MQ=255)
                cTACTGCAACATCCCTTTCGAACAACGGTAGCCCGGGAGCAGGCACGTGACGATTTCGGTGAAACCGTAGGTGCCATACTCGATACGGTAAGc                                                                    >  2:190989/1‑93 (MQ=255)
                cTACTGCAACATCCCTTTCGAACAACGGTAGCCCGGGAGCAGGCACGTGACGATTTCGGTGAAACCGTAGGTGCCATACTCGATACGGTAAGc                                                                    >  2:830673/1‑93 (MQ=255)
                   cTGCAACATCCCTTTCGAACAACGGTAGCCCGGGAGCAGGCACGTGACGATTTCGGTGAAACCGTAGGTGCCATACTCGATACGGTAAGCAcc                                                                 >  1:1044869/1‑93 (MQ=255)
                   cTGCAACATCCCTTTCGAACAACGGTAGCCCGGGAGCAGGCACGTGACGATTTCGGTGAAACCGTAGGTGCCATACTCGATACGGTAAGCAcc                                                                 <  1:4601541/93‑1 (MQ=255)
                    tGCAACATCCCTTTCGAACAACGGTAGCCCGGGAGCAGGCACGTGACGATTTCGGTGAAACCGTAGGTGCCATACTCGATACGGTAAGCACCt                                                                <  1:3640436/93‑1 (MQ=255)
                    tGCAACATCCCTTTCGAACAACGGTAGCCCGGGAGCAGGCACGTGACGATTTCGGTGAAACCGTAGGTGCCATACTCGATACGGTAAGCACCt                                                                >  1:6333401/1‑93 (MQ=255)
                     gCAACATCCCTTTCGAACAACGGTAGCCCGGGAGCAGGCACGTGACGATTTCGGTGAAACCGTAGGTGCCATACTCGATACGGTAAGCACCtt                                                               <  2:1548423/93‑1 (MQ=255)
                     gCAACATCCCTTTCGAACAACGGTAGCCCGGGAGCAGGCACGTGACGATTTCGGTGAAACCGTAGGTGCCATACTCGATACGGTAAGCACCtt                                                               <  2:2551629/93‑1 (MQ=255)
                      gaaCATCCCTTTCGAACAGCGGCACCCCGGTAGCAGGCAGTTGACGATTTGGGTTAAACGGTATGTGCCATTCTCGAGACGGTAAGCACCttt                                                              <  2:2930937/92‑1 (MQ=255)
                      cAACATCCCTTTCGAACAACGGTAGCCCGGGAGCAGGCACGTGACGATTTCGGTGAAACCGTAGGTGCCATACTCGATACGGTAAGCACCttt                                                              <  1:2595794/93‑1 (MQ=255)
                       aaCATCCCTTTCGAACAACGGTAGCCCGGGAGCAGGCACGTGACGATTTCGGTGAAACCGTAGGTGCCATACTCGATACGGTAAGCACCTTTc                                                             >  2:5015934/1‑93 (MQ=255)
                       aaCATCCCTTTCGAACAACGGTAGCCCGGGAGCAGGCACGTGACGATTTCGGTGAAACCGTAGGTGCCATACTCGATACGGTAAGCACCTTTc                                                             <  1:288997/93‑1 (MQ=255)
                       aaCATCCCTTTCGAACAACGGTAGCCCGGGAGCAGGCACGTGACGATTTCGGTGAAACCGTAGGTGCCATACTCGATACGGTAAGCACCTTTc                                                             >  2:3405035/1‑93 (MQ=255)
                       aaCATCCCTTTCGAACAACGGTAACCCGGGAGCAGGCACGTGACGATTTCGGTGAAACCGTAGGTGCCATACTCGCTACGGTAAGCACCTTTc                                                             >  2:3029056/1‑93 (MQ=255)
                        aCATCCCTTTCGAACAACGGTAGCCCGGGAGCAGGCACGTGACGATTTCGGTGAAACCGTAGGTGCCATACTCGATACGGTAAGCACCTTTcc                                                            >  2:126561/1‑93 (MQ=255)
                         cATCCCTTTCGAACAACGGTAGCCCGGGAGCAGGCACGTGACGATTTCGGTGAAACCGTAGGTGCCATACTCGATACGGTAAGCACCTTTccc                                                           >  1:4392536/1‑93 (MQ=255)
                          aTCCCTTTCGAACAACGGTAGCCCGGGAGCAGGCACGTGACGATTTCGGTGAAACCGTAGGTGCCATACTCGATACGGTAAGCACCTTTcccc                                                          <  2:1750976/93‑1 (MQ=255)
                          aTCCCTTTCGAACAACGGTAGCCCGGGAGCAGGCACGTGACGATTTCGGTGAAACCGTAGGTGCCATACTCGATACGGTAAGCACCTTTcccc                                                          <  1:5094772/93‑1 (MQ=255)
                           tCCCTTTCGAACAACGGTAGCCCGGGAGCAGGCACGTGACGATTTCGGTGAAACCGTAGGTGCCATACTCGATACGGTAAGCACCTTTCCCCt                                                         >  2:5342257/1‑93 (MQ=255)
                            cccTTTCGAACAACGGTAGCCCGGGAGCAGGCACGTGACGATTTCGGTGAAACCGTAGGTGCCATACTCGATACGGTAAGCACCTTTCCCCtt                                                        >  1:2515491/1‑93 (MQ=255)
                             ccTTTCGAACAACGGTAGCCCGGGAGCAGGCACGTGACGATTTCGGTGAAACCGTAGGTGCCATACTCGATACGGTAAGCACCTTTCCCCttt                                                       >  2:2796289/1‑92 (MQ=255)
                             ccTTTCGAACAACGGTAGCCCGGGAGCAGGCACGTGACGATTTCGGTGAAACCGTAGGTGCCATACTCGATACGGTAAGCACCTTTCCCCTTa                                                       <  1:2642339/93‑1 (MQ=255)
                               tttCGAACAACGGTAGCCCGGGAGCAGGCACGTGACGATTTCGGTGAAACCGTAGGTGCCATACTCGATACGGTAAGCACCTTTCCCCTTAtt                                                     <  2:688981/93‑1 (MQ=255)
                               tttCGAACAACGGTAGCCCGGGAGCAGGCACGTGACGATTTCGGTGAAACCGTAGGTGCCATACTCGATACGGTAAGCACCTTTCCCCTTAtt                                                     <  2:5245858/93‑1 (MQ=255)
                               tttCGAACAACGGTAGCCCGGGAGCAGGCACGTGACGATTTCGGTGAAACCGTAGGTGCCATACTCGATACGGTAAGCACCTTTCCCCTTAtt                                                     >  1:655792/1‑93 (MQ=255)
                                      caacggTAGCCCGGGAGCAGGCACGTGACGATTTCGGTGAAACCGTAGGTGCCATACTCGATACGGTAAGCACCTTTCCCCTTATTCCCGccc                                              <  2:4498775/89‑1 (MQ=255)
                                         cggTAGCCCGGGAGCAGGCACGTGACGATTTCGGTGAAACCGTAGGTGCCATACTCGATACGGTAAGCACCTTTCCCCTTATTCCCGCCCCgg                                           <  2:4678168/92‑1 (MQ=255)
                                           gTAGCCCGGGAGCAGGCACGTGACGATTTCGGTGAAACCGTAGGTGCCATACTCGATACGGTAAGCACCTTTCCCCTTATTCCCACCCCGGTg                                         <  2:6302916/93‑1 (MQ=255)
                                              gCCCGGGAGCAGGCACGTGACGATTTCGGTGAAACCGTAGGTGCCATACTCGATACGGTAAGCACCTTTCCCCTTATTCCCGCCCCGGTGCgg                                      <  1:5345559/93‑1 (MQ=255)
                                              gCCCGGGAGCAGGCACGTGACGATTTCGGTGAAACCGTAGGTGCCATACTCGATACGGTAAGCACCTTTCCCCTTATTCCCGCCCCGGTGCgg                                      >  2:136605/1‑93 (MQ=255)
                                                ccGGGAGCAGGCACGTGACGATTTCGGTGAAACCGTAGGTGCCATACTCGATACGGTAAGCACCTTTCCCCTTATTCCCGCCCCGGTGCGGAc                                    >  1:2558177/1‑93 (MQ=255)
                                                  gggAGCAGGCACGTGACGATTTCGGTGAAACCGTAGGTGCCATACTCGATACGGTAAGCACCTTTCCCCTTATTCCCGCCCCGGTGCGGACCa                                  <  1:2857983/93‑1 (MQ=255)
                                                  gggAGCAGGCACGGGACGATTTCGGTGAAACCGTAGGTGCCATACTCGATACGGTAAGCACCTTTCCCCTTATTCCCGCCCCGGTGCGGACCa                                  >  2:5688178/1‑93 (MQ=255)
                                                   ggAGCAGGCACGTGACGATTTCGGTGAAACCGTAGGTGCCATACTCGATACGGTAAGCACCTTTCCCCTTATTCCCGCCCCGGTGCGGACCat                                 <  1:3474318/93‑1 (MQ=255)
                                                   ggAGCAGGCACGTGACGATTTCGGTGAAACCGTAGGTGCCATACTCGATACGGTAAGCACCTTTCCCCTTATTCCCGCCCCGGTGCGGACCat                                 >  1:6232312/1‑93 (MQ=255)
                                                     aGCAGGCACGTGACGATTTCGGTGAAACCGTAGGTGCCATACTCGATACGGTAAGCACCTTTCCCCTTATTCCCGCCCCGGTGCGGACCatta                               <  1:2037363/93‑1 (MQ=255)
                                                       cAGGCACGTGACGATTTCGGTGAAACCGTAGGTGCCATACTCGATACGGTAAGCACCTTTCCCCTTATTCCCGCCCCGGTGCGGACCattatt                             >  2:228585/1‑93 (MQ=255)
                                                         ggCCCGTGACGATTTCGGTGAACCCGTAGGTGCCATACTCGATACGGTAAGCACCTTTCCCCTTATTCCCGCCCCGGTGCGGACCATTATTCa                           <  2:3843895/93‑1 (MQ=37)
                                                         ggCACGTGACGATTTCGGTGAAACCGTAGGTGCCATACTCGATACGGTAAGCACCTTTCCCCTTATTCCCGCCCCGGTGCGGACCATTATTCa                           <  1:4161485/93‑1 (MQ=255)
                                                         ggCACGTGACGATTTCGGTGAAACCGTAGGTGCCATACTCGATACGGTAAGCACCTTTCCCCTTATTCCCGCCCCGGTGCGGACCATTATTCa                           >  1:6226308/1‑93 (MQ=255)
                                                         ggCACGTGACGATTTCGGTGAAACCGTAGGTGCCATACTCGATACGGTAAGCACCTTTCCCCTTATTCCCGCCCCGGTGCGGACCATTATTCa                           <  2:5937045/93‑1 (MQ=255)
                                                          gCACGGGACGATTTCGGTGAAACCGTAGGTGCCATACTCGATACGGTAAGCACCTTTCCCCTTATTCCCGCCCCGGTGCGGACCATTATTCAg                          <  1:1390982/93‑1 (MQ=255)
                                                            aCGTGACGATTTCGGTGAAACCGTAGGTGCCATACTCGATACGGTAAGCACCTTTCCCCTTATTCCCGCCCCGGTGCGGACCATTATTCAGGc                        <  1:5691178/93‑1 (MQ=255)
                                                              gTGACGATTTCGGTGAAACCGTAGGTGCCATACTCGATACGGTAAGCACCTTTCCCCTTATTCCCGCCCCGGTGCGGACCATTATTCAGGCCg                      >  1:4031185/1‑93 (MQ=255)
                                                              gTGACGATTTCGGTGAAACCGTAGGTGCCATACTCGATACGGTAAGCACCTTTCCCCTTATTCCCGCCCCGGTGCGGACCATTATTCAGGCCg                      >  1:5736651/1‑93 (MQ=255)
                                                               tGACGATTTCGGTGAAACCGTAGGTGCCATACTCGATACGGTAAGCACCTTTCCCCTTATTCCCGCCCCGGTGCGGACCATTATTCAGGCCGt                     >  2:4826055/1‑93 (MQ=255)
                                                                gACGATTTCGGTGAAACCGTAGGTGCCATACTCGATACGGTAAGCACCTTTCCCCTTATTCCCGCCCCGGTGCGGACCATTATTCAGGCCGTg                    <  1:1216680/93‑1 (MQ=255)
                                                                 aCGATTTCGGTGAAACCGTAGGTGCCATACTCGATACGGTAAGCACCTTTCCCCTTATTCCCGCCCCGGTGCGGACCATTATTCAGGCCGTGc                   >  1:3896149/1‑93 (MQ=255)
                                                                 aCGATTTCGGTGAAACCGTAGGTGCCATACTCGATACGGTAAGCACCTTTCCCCTTATTCCCGCCCCGGTGCGGACCATTATTCAGGCCGTGc                   >  1:3080581/1‑93 (MQ=255)
                                                                 aCGATGTCGGTAAAACCGTAGGTGCCATACTCGATACGGTAAGCACCTTTCCCCTTATTCCCGCCCCGGTGCGGACCATTATTCAGGCCGTGc                   <  2:3738568/93‑1 (MQ=255)
                                                                    aTTTCGGTGAAACCGTAGGTGCCATACTCGATACGGTAAGCACCTTTCCCCTTATTCCCGCCCCGGTGCGGACCATTATTCAGGCCGTGCGtt                >  2:2391674/1‑93 (MQ=255)
                                                                     tttCGGTGAAACCGTAGGTGCCATACTCGATACGGTAAGCACCTTTCCCCTTATTCCCGCCCCGGTGCGGACCATTATTCAGGCCGTGCGTTc               >  1:115578/1‑93 (MQ=255)
                                                                     tttCGGTGAAACCGTAGGTGCCATACTCGATACGGTAAGCACCTTTCCCCTTATTCCCGCCCCGGTGCGGACCATTATTCAGGCCGTGCGTTc               >  1:1042149/1‑93 (MQ=255)
                                                                     tttCGGTGAAACCGTAGGTGCCATACTCGATACGGTAAGCACCTTTCCCCTTATTCCCGCCCCGGTGCGGACCATTATTCAGGCCGTGCGTTc               >  2:3426246/1‑93 (MQ=255)
                                                                     tttCGGTGAAACCGTAGGTGCCATACTCGATACGGTAAGCACCTTTCCCCTTATTCCCGCCCCGGTGCGGACCATTATTCAGGCCGTGCGTTc               >  1:5008087/1‑93 (MQ=255)
                                                                     tttCGGTGAAACCGTAGGTGCCATACTCGATACGGTAAGCACCTTTCCCCTTATTCCCGCCCCGGTGCGGACCATTATTCAGGCCGTGCGTTc               >  1:5550957/1‑93 (MQ=255)
                                                                     tttCGGTGAAACCGTAGGTGCCATACTCGATACGGTAAGCACCTTTCCCCTTATTCCCGCCCCGCTGCGGACCATTATTCAGGCCGTGCGGTc               >  2:1829687/1‑93 (MQ=255)
                                                                      ttCGGTGAAACCGTAGGTGCCATACTCGATACGGTAAGCACCTTTCCCCTTATTCCCGCCCCGGTGCGGACCATTATTCAGGCCGTGCGTTcc              <  1:5530577/93‑1 (MQ=255)
                                                                      ttCGGTGAAACCGTAGGTGCCATACTCGATACGGTAAGCACCTTTCCCCTTATCCCCGCCCCGGTGCGGACCATTATTCAGGCCGTGCGTTcc              <  1:4173582/93‑1 (MQ=255)
                                                                        cGGTGAAACCGTAGGTGCCATACTCGATACGGTAAGCACCTTTCCCCTTATTCCCGCCCCGGTGCGGACCATTATTCAGGCCGTGCGTTCCTc            <  2:2687349/93‑1 (MQ=255)
                                                                        cGGTGAAACCGTAGGTGCCATACTCGATACGGTAAGCACCTTTCCCCTTATTCCCGCCCCGGTGCGGACCATTATTCAGGCCGTGCGTTCCTc            >  2:5122966/1‑93 (MQ=255)
                                                                        cGGTGAAACCGTAGGTGCCATACTCGATACGGTAAGCACCTTTCCCCTTATTCCCGCCCCGGTGCGGACCATTATTCAGGCCGTGCGTTCCTc            <  2:5636584/93‑1 (MQ=255)
                                                                         gttGAAACCGTAGGTGCCATACTCGATACGGTAAGCACCTTTCCCCTTATTCCCGCCCCGGTGCGGACCATTATTCAGGCCGTGCGTTCCTCg           <  2:5431933/91‑1 (MQ=255)
                                                                          gTGAAACCGTAGGTGCCATACTCGATACGGTAAGCACCTTTCCCCTTATTCCCGCCCCGGTGCGGACCATTATTCAGGCCGTGCGTTCCTCgg          <  1:2232630/93‑1 (MQ=255)
                                                                          gTGAAACCGTAGGTGCCATACTCGATACGGTAAGCACCTTTCCCCTTATTCCCGCCCCGGTGCGGACCATTATTCAGGCCGTGCGTTCCTCgg          <  2:1239342/93‑1 (MQ=255)
                                                                           tGAAACCGTAGGTGCCATACTCGATACGGTAAGCACCTTTCCCCTTATTCCCGCCCCGGTGCGGACCATTATTCAGGCCGTGCGTTCCTCggt         >  1:2716016/1‑93 (MQ=255)
                                                                            gAAACCGTAGGTGCCATACTCGATACGGTAAGCACCTTTCCCCTTATTCCCGCCCCGGTGCGGACCATTATTCAGGCCGTGCGTTCCTCggtg        >  1:3427089/1‑93 (MQ=255)
                                                                               aCCGTAGGTGCCATACTCGATACGGTAAGCACCTTTCCCCTTATTCCCGCCCCGGTGCGGACCATTATTCAGGCCGTGCGTTCCTCGgtggtg     >  1:4880847/1‑93 (MQ=255)
                                                                                ccGTAGGTGCCATACTCGATACGGTAAGCACCTTTCCCCTTATTCCCGCCCCGGTGCGGACCATTATTCAGGCCGTGCGTTCCTCGGTGgtgt    >  1:904342/1‑93 (MQ=255)
                                                                                  gTAGGTGCCATACTCGATACGGTAAGCACCTTTCCCCTTATTCCCGCCCCGGTGCGGACCATTATTCAGGCCGTGCGTTCCTCGGTGGTGTCa  <  2:1065646/93‑1 (MQ=255)
                                                                                  gTAGGTGCCATACTCGATACGGTAAGCACCTTTCCCCTTATTCCCGCCCCGGTGCGGACCATTATTCAGGCCGTGCGTTCCTCGGTGGTGTCa  >  1:1582678/1‑93 (MQ=255)
                                                                                  gTAGGTGCCATACTCGATACGGTAAGCACCTTTCCCCTTATTCCCGCCCCGGTGCGGACCATTATTCAGGCCGTGCGTTCCTCGGTGGTGTCa  <  2:5606300/93‑1 (MQ=255)
                                                                                  gTAGGTGCCATACTCGATACGGTAAGCACCTTTCCCCTTATTCCCGCCCCGGTGCGGACCATTATTCAGGCCGTGCGTTCCTCGGTGGTGTCa  <  2:1640246/93‑1 (MQ=255)
                                                                                      |                                                                                        
CAGCCCGGTAGTAGCGCTACTGCAACATCCCTTTCGGACATGGGTAGCCCGGGAGCAGGCACGTGACGATTTCGGTGAAACCGTAGATACCATACTCGATACGGTAAGCACCTTTCCCCTTATTCCCGCCCCGGTGCGGACCATTATTCAGGCCGTGCGTTCCTCGGTGGTGTCA  >  NC_000913/2766387‑2766561

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 19 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
AGCCCAGCAGCCCGGTAGTAGCGCTACTGCAACATCCCTTTCGGACATGGGTAGCCCGGGAGCAGGCACGTGACGATTTCGGTGAAACCGTAGATACCATACTCGATACGGTAAGCACCTTTCCCCTTATTCCCGCCCCGGTGCGGACCATTATTCAGGCCGTGCGTTCCTCGGTGGTGTCAG  >  NC_000913/2766380‑2766562
                                                                                             |                                                                                         
AGGCCAGCAGCCCGGTAGTAGCGCTACTGCAACATCCCTTTCGAACAACGGTAGCCCGGGAGCAGGCACGTGACGATTTCGGTGAAACCGTAGG                                                                                           <  SRR4302537.1134855/94‑1 (MQ=60)
AGGCCAGCAGCCCGGTAGTAGCGCTACTGCAACATCCCTTTCGAACAACGGTAGCCCGGGAGCAGGCACGTGACGATTTCGGTGAAACCGTAGG                                                                                           <  SRR4302537.401246/94‑1 (MQ=60)
 agCCAGCAGCCCGGTAGTAGCGCTACTGCAACATCCCTTTCGAACAACGGTAGCCCGGGAGCAGGCACGTGACGATTTCGGTGAAACCGTAGGT                                                                                          <  SRR4302537.5373420/92‑1 (MQ=60)
 GGCCAGCAGCCCGGTAGTAGCGCTACTGCAACATCCCTTTCGAACAACGGTAGCCCGGGAGCAGGCACGTGACGATTTCGGTGAAACCGTAGGT                                                                                          <  SRR4302537.6216317/94‑1 (MQ=60)
      GCAGCCCGGTAGTAGCGCTACTGCAACATCCCTTTCGAACAACGGTAGCCCGGGAGCAGGCACGTGACGATTTCGGTGAAACCGTAGGTGCCAT                                                                                     <  SRR4302537.881190/94‑1 (MQ=60)
        AGCCCGGTAGTAGCGCTACTGCAACATGCCTTTCGAACAACGGTAGCCCGGGAGCAGGCACGTGACGATTTCGGTGAAACCGTAGGTGCCATAC                                                                                   <  SRR4302537.4354534/94‑1 (MQ=60)
        AGCCCGGTAGTAGCGCTACTGCAACATCCCTTTCGAACAACGGTAGCCCGGGAGCAGGCACGTGACGATTTCGGTGAAACCGTAGGTGCCATAC                                                                                   <  SRR4302537.822594/94‑1 (MQ=60)
        AGCCCGGTAGTAGCGCTACTGCAACATCCCTTTCGAACAACGGTAGCCCGGGAGCAGGCACGTGACGATTTCGGTGAAACCGTAGGTGCCATAC                                                                                   >  SRR4302537.816561/1‑94 (MQ=60)
         GCCCGGTAGTAGCGCTACTGCAACATCCCTTTCGAACAACGGTAGCCCGGGAGCAGGCACGTGACGATTTCGGTGAAACCGTAGGTGCCATACT                                                                                  >  SRR4302537.52997/1‑94 (MQ=60)
           CCGGTAGTAGCGCTACTGCAACATCCCTTTCGAACAACGGTAGCCCGGGAGCAGGCACGTGACGATTTCGGTGAAACCGTAGGTGCCATACTCG                                                                                <  SRR4302537.2616685/94‑1 (MQ=60)
           CCGGTAGTAGCGCTACTGCAACATCCCTTTCGAACAACGGTAGCCCGGGAGCAGGCACGCGACGATTTCGGTGAAACCGTAGGTGCCATACTTG                                                                                >  SRR4302537.4378953/1‑94 (MQ=60)
            CGGTAGTAGCGCTACTGCAACATCCCTTTCGAACAACGGTAGCCCGGGAGCAGGCACGTGACGATTTCGGTGAAACCGTAGGTGCCATACTCGA                                                                               >  SRR4302537.3690244/1‑94 (MQ=60)
             GGTAGTAGCGCTACTGCAACATCCCTTTCGAACAACGGTAGCCCGGGAGCAGGCACGTGACGATTTCGGTGAAACCGTAGGTGCCATACTCGAT                                                                              <  SRR4302537.6442703/94‑1 (MQ=60)
               TAGTAGCGCTACTGCAACATCCCTTTCGAACAACGGTAGCCCGGGAGCAGGCACGTGACGATTTCGGTGAAACCGTAGGTGCCATACTCGATAC                                                                            >  SRR4302537.1566720/1‑94 (MQ=60)
               TAGTAGCGCTACTGCAACATCCCTTTCGAACAACGGTAGCCCGGGAGCAGGCACGTGACGATTTCGGTGAAACCGTAGGTGCCATACTCGATAC                                                                            <  SRR4302537.575184/94‑1 (MQ=60)
               TAGTAGCGCTACTGCAACATCCCTTTCGAACAACGGTAGCCCGGGAGCAGGCACGTGACGATTTCGGTGAAACCGTAGGTGCCATACTCGATAC                                                                            <  SRR4302537.2882067/94‑1 (MQ=60)
                 GTAGCGCTACTGCAACATCCCTTTCGAACAACGGTAGCCCGGGAGCAGGCACGTGACGATTTCGGTGAAACCGTAGGTGCCATACTCGATACGG                                                                          >  SRR4302537.5331502/1‑94 (MQ=60)
                   AGCGCTACTGCAACATCCCTTTCGAACAACGGTAGCCCGGGAGCAGGCACGTGACGATTTCGGTGAAACCGTAGGTGCCATACTCGATACGGTA                                                                        <  SRR4302537.1912032/94‑1 (MQ=60)
                   AGCGCTACTGCAACATCCCTTTCGAACAACGGTAGCCCGGGAGCAGGCACGTGACGATTTCGGTGAAACCGTAGGTGCCATACTCGATACGGTA                                                                        >  SRR4302537.4246994/1‑94 (MQ=60)
                    GCGCTACTGCAACATCCCTTTCGAACAACGGTAGCCCGGGAGCAGGCACGTGACGATTTCGGTGAAACCGTAGGTGCCATACTCGATACGGTAA                                                                       <  SRR4302537.3059432/94‑1 (MQ=60)
                     CGCTACTGCAACATCCCTTTCGAACAACGGTAGCCCGGGAGCAGGCACGTGACGATTTCGGTGAAACCGTAGGTGCCATACTCGATACGGTAAG                                                                      <  SRR4302537.2670508/94‑1 (MQ=60)
                      GCTACTGCAACATCCCTTTCGAACAACGGTAGCCCGGGAGCAGGCACGTGACGATTTCGGTGAAACCGTAGGTGCCATACTCGATACGGTAAGC                                                                     <  SRR4302537.2416545/94‑1 (MQ=60)
                       CTACTGCAACATCCCTTTCGAACAACGGTAGCCCGGGAGCAGGCACGTGACGATTTCGGTGAAACCGTAGGTGCCATACTCGATACGGTAAGCA                                                                    >  SRR4302537.2392656/1‑94 (MQ=60)
                         ACTGCAACATCCCTTTCGAACAACGGTAGCCCGGGAGCAGGCACGTGACGATTTCGGTGAAACCGTAGGTGCCATACTCGATACGGTAAGCACC                                                                  >  SRR4302537.1052081/1‑94 (MQ=60)
                         ACTGCAACATCCCTTTCGAACAACGGTAGCCCGGGAGCAGGCACGTGACGATTTCGGTGAAACCGTAGGTGCCATACTCGATACGGTAAGCACC                                                                  <  SRR4302537.1223568/94‑1 (MQ=60)
                          CTGCAACATCCCTTTCGAACAACGGTAGCCCGGGAGCAGGCACGTGACGATTTCGGTGAAACCGTAGGTGCCATACTCGATACGGTAAGCACCT                                                                 >  SRR4302537.6376705/1‑94 (MQ=60)
                          CTGCAACATCCCTTTCGAACAACGGTAGCCCGGGAGCAGGCACGTGACGATTTCGGTGAAACCGTAGGTGCCATACTCGATACGGTAAGCACCT                                                                 <  SRR4302537.4633392/94‑1 (MQ=60)
                           TGCAACATCCCTTTCGAACAACGGTAGCCCGGGAGCAGGCACGTGACGATTTCGGTGAAACCGTAGGTGCCATACTCGATACGGTAAGCACCTT                                                                <  SRR4302537.3666078/94‑1 (MQ=60)
                             CAACATCCCTTTCGAACAACGGTAGCCCGGGAGCAGGCACGTGACGATTTCGGTGAAACCGTAGGTGCCATACTCGATACGGTAAGCACCTTTC                                                              <  SRR4302537.2613954/94‑1 (MQ=60)
                              AACATCCCTTTCGAACAACGGTAGCCCGGGAGCAGGCACGTGACGATTTCGGTGAAACCGTAGGTGCCATACTCGATACGGTAAGCACCTTTCC                                                             <  SRR4302537.291004/94‑1 (MQ=60)
                              AACATCCCTTTCGAACAACGGTAGCCCGGGAGCAGGCACGTGACGATTTCGGTGAAACCGTAGGTGCCATACTCGATACGGTAAGCACCTTTCC                                                             >  SRR4302537.3465382/1‑94 (MQ=60)
                               ACATCCCTTTCGAACAACGGTAGCCCGGGAGCAGGCACGTGACGATTTCGGTGAAACCGTAGGTGCCATACTCGATACGGTAAGCACCTTTCCC                                                            >  SRR4302537.4422844/1‑94 (MQ=60)
                                 ATCCCTTTCGAACAACGGTAGCCCGGGAGCAGGCACGTGACGATTTCGGTGAAACCGTAGGTGCCATACTCGATACGGTAAGCACCTTTCCCCT                                                          <  SRR4302537.106973/94‑1 (MQ=60)
                                 ATCCCTTTCGAACAACGGTAGCCCGGGAGCAGGCACGTGACGATTTCGGTGAAACCGTAGGTGCCATACTCGATACGGTAAGCACCTTTCCCCT                                                          <  SRR4302537.5129857/94‑1 (MQ=60)
                                 ATCCCTTTCGAACAACGGTAGCCCGGGAGCAGGCACGTGACGATTTCGGTGAAACCGTAGGTGCCATACTCGATACGGTAAGCACCTTTCCCCT                                                          >  SRR4302537.5114985/1‑94 (MQ=60)
                                  tccctatctaacaacGGTAGCCCGGGAGCAGGCACGTGACGATTTCGGTGAAACCGTAGGTGCCATCCTCGATACGGTAAGCACCTTTCCCCTT                                                         <  SRR4302537.1986654/79‑1 (MQ=60)
                                  TCCCTTTCGAACAACGGTAGCCCGGGAGCAGGCACGTGACGATTTCGGTGAAACCGTAGGTGCCATACTCGATACGGTAAGCACCTTTCCCCTT                                                         >  SRR4302537.2533168/1‑94 (MQ=60)
                                   CCCTTTCGAACAACGGTAGCCCGGGAGCAGGCACGTGACGATTTCGGTGAAACCGTAGGTGCCATACTCGATACGGTAAGCACCTTTCCCCTTA                                                        >  SRR4302537.5800156/1‑94 (MQ=60)
                                    CCTTTCGAACAACGGTAGCCCGGGAGCAGGCACGTGACGATTTCGGTGAAACCGTAGGTGCCATACTCGATACGGTAAGCACCTTTCCCCTTAT                                                       >  SRR4302537.5278092/1‑94 (MQ=60)
                                    CCTTTCGAACAACGGTAGCCCGGGAGCAGGCACGTGACGATTTCGGTGAAACCGTAGGTGCCATACTCGATACGGTAAGCACCTTTCCCCTTAT                                                       <  SRR4302537.2660826/94‑1 (MQ=60)
                                    CCTTTCGAACAACGGTAGCCCGGGAGCAGGCACGTGACGATTTCGGTGAAACCGTAGGTGCCATACTCGATACGGTAAGCACCTTTCCCCTTAT                                                       <  SRR4302537.2590667/94‑1 (MQ=60)
                                     CTTTCGAACAACGGTAGCCCGGGAGCAGGCACGTGACGATTTCGGTGAAACCGTAGGTGCCATACTCGATACGGTAAGCACCTTTCCCCTTATT                                                      >  SRR4302537.3604887/1‑94 (MQ=60)
                                     CTTTCGAACAACGGTAGCCCGGGAGCAGGCACGTGACGATTTCGGTGAAACCGTAGGTGCCATACTCGATACGGTAAGCACCTTTCCCCTTATT                                                      >  SRR4302537.660263/1‑94 (MQ=60)
                                      tttcgagcaacGGTAGCCCGGGAGCAGGCACGTGACGATTTCGGTGAAACCGTAGGTGCCATACTCGATACGGTAAGCACCTTTCCCCTTATTC                                                     <  SRR4302537.2296656/83‑1 (MQ=60)
                                         cgaacaacGGTAGCCCGGGAGCAGGCACGTGACGATTTCGGTGAAACCGTAGGTGCCATACTCGATACGGTAAGCACCTTTCCCCTTATTCCCG                                                  <  SRR4302537.4659774/86‑1 (MQ=60)
                                          gaacaacGGTAGCCCGGGAGCAGGCACGTGACGATTTCGGTGAAACCGTAGGTGCCATACTCGATACGGTAAGCACCTTTCCCCTTATTCCCGC                                                 <  SRR4302537.2748664/87‑1 (MQ=60)
                                           aacaacGGTAGCCCGGGAGCAGGCACGTGACGATTTCGGTGAAACCGTAGGTGCCATACTCGATACGGTAAGCACCTTTCCCCTTATTCCCGCC                                                >  SRR4302537.2264042/7‑94 (MQ=60)
                                              aacGGTAGCCCGGGAGCAGGCACGTGACGATTTCGGTTAAACCGTAGGTGCCATACTCGATACGGTAAGCACCTTTCCCCTTATTCCCGCCCCG                                             <  SRR4302537.918060/91‑1 (MQ=60)
                                                  GTAGCGCGGGAGCAGCCACGGGACGATTTCGGTGGAGCCGTAGGTGCCATACTCGATACGGTAAGCACCTTTCCCCTTATTCCCGCCCCGGTGC                                         <  SRR4302537.79355/94‑1 (MQ=60)
                                                    AGCCCGGGAGCAGGCACGTGACGATTTCGGTGAAACCGTAGGCGCCATACTCGATACGGTAAGCAGCTTTCCACTTATTCCCGCCCCGGTGCGG                                       >  SRR4302537.3450387/1‑94 (MQ=60)
                                                     GCCCGGGAGCAGGCACGTGACGATTTCGGTGAAACCGTAGGTGCCATACTCGATACGGTAAGCACCTTTCCCCTTATTCCCGCCCCGGTGCGGA                                      <  SRR4302537.5382184/94‑1 (MQ=60)
                                                      CCCGGGAGCAGGCACGTGACGATTTCGGTGAAACCGTAGGTGCCATACTCGATACGGTAAGCACCTTTCCCCTTATTCCCGCCCCGGTGCGGAC                                     >  SRR4302537.2576108/1‑94 (MQ=60)
                                                       CCGGGAGCAGGCGCGTGTCGATTTCGGTGAAACCGTAGGTGCCATACTCGATACGGTAAGCACCTTTCCCCTTATTCCCGCCCCGGTGCGGACC                                    <  SRR4302537.4029867/94‑1 (MQ=60)
                                                         GGGAGCAGGCACGTGACGATTTCGGTGAAACCGTAGGTGCCATACTCGATACGGTAAGCACCTTTCCCCTTATTCCCGCCCCGGTGCGGACCAT                                  >  SRR4302537.6275178/1‑94 (MQ=60)
                                                         GGGAGCAGGCACGTGACGATTTCGGTGAAACCGTAGGTGCCATACTCGATACGGTAAGCACCTTTCCCCTTATTCCCGCCCCGGTGCGGACCAT                                  <  SRR4302537.2878021/94‑1 (MQ=60)
                                                          GGAGCAGGCACGTGACGATTTCGGTGAAACCGTAGGTGCCATACTCGATACGGTAAGCACCTTTCCCCTTATTCCCGCCCCGGTGCGGACCATT                                 <  SRR4302537.2926570/94‑1 (MQ=60)
                                                          GGAGCAGGCACGTGACGATTTCGGTGAAACCGTAGGTGCCATACTCGATACGGTAAGCACCTTTCCCCTTATTCCCGCCCCGGTGCGGACCATT                                 <  SRR4302537.3498901/94‑1 (MQ=60)
                                                            AGCAGGCACGTGACGATTTCGGTGAAACCGTAGGTGCCATACTCGATACGGTAAGCACCTTTCCCCTTATTCCCGCCCCGGTGCGGACCATTAT                               <  SRR4302537.2051568/94‑1 (MQ=60)
                                                              CAGGCACGTGACGATTTCGGTGAAACCGTAGGTGCCATACTCGATACGGTAAGCACCTTTCCCCTTATTCCCGCCCCGGTGCGGACCATTATTC                             <  SRR4302537.2444687/94‑1 (MQ=60)
                                                               AGGCACGTGACGATTTCGGTGAAACCGTAGGTGCCATACTCGATACGGTAAGCACCTTTCCCCTTATTCCCGCCCCGGTGCGGACCATTATTCA                            >  SRR4302537.4864249/1‑94 (MQ=60)
                                                               AGGCACGTGACGATTTCGGTGAAACCGTAGGTGCCATACTCGATACGGTAAGCACCTTTCCCCTTATTCCCGCCCCGGTGCGGACCATTATTCA                            >  SRR4302537.6269154/1‑94 (MQ=60)
                                                                GGCACGTGACGATTTCGGTGAAACCGTAGGTGCCATACTCGATACGGTAAGCACCTTTCCCCTTATTCCCGCCCCGGTGCGGACCATTATTCAG                           <  SRR4302537.4190547/94‑1 (MQ=60)
                                                                 GCACGTGACGATTTCGGTGAAACCGTAGGTGCCATACTCGATACGGTAAGCACCTTTCCCCTTATTCCCGCCCCGGTGCGGACCATTATTCAGG                          <  SRR4302537.5600416/94‑1 (MQ=60)
                                                                 GCACGTGACGATTTCGGTGAAACCGTAGGTGCCATACTCGATACGGTAAGCACCTTTCCCCTTATTCCCGCCCCGGTGCGGACCATTATTCAGG                          >  SRR4302537.3861292/1‑94 (MQ=60)
                                                                 GCACGGGACGATTTCGGTGAAACCGTAGGTGCCATACTCGATACGGTAAGCACCTTTCCCCTTATTCCCGCCCCGGTGCGGACCATTATTCAGG                          <  SRR4302537.1400683/94‑1 (MQ=60)
                                                                   ACGTGACGATTTCGGTGAAACCGTAGGTGCCATACTCGATACGGTAAGCACCTTTCCCCTTATTCCCGCCCCGGTGCGGACCATTATTCAGGCC                        <  SRR4302537.5730105/94‑1 (MQ=60)
                                                                    CGTGACGATTTCGGTGAAACCGTAGGTGCCATACTCGATACGGTAAGCACCTTTCCCCTTATTCCCGCCCCGGTGCGGACCATTATTCAGGCCG                       >  SRR4302537.5775927/1‑94 (MQ=60)
                                                                    CGTGACGATTTCGGTGAAACCGTAGGTGCCATACTCGATACGGTAAGCACCTTTCCCCTTATTCCCGCCCCGGTGCGGACCATTATTCAGGCCG                       >  SRR4302537.4059401/1‑94 (MQ=60)
                                                                      TGACGATTTCGGTCAAACCGTAGGTGCCATACTCGATACGGTAAGCACCTTTCCCCTTATTCCCGCCCCGGTGCGGACCATTATTCAGGCCGTG                     <  SRR4302537.864300/94‑1 (MQ=60)
                                                                       GACGATTTCGGTGAAACCGTAGGTGCCATACTCGATACGGTAAGCACCTTTCCCCTTATTCCCGCCCCGGTGCGGACCATTATTCAGGCCGTGC                    >  SRR4302537.3102035/1‑94 (MQ=60)
                                                                       GACGATTTCGGTGAAACCGTAGGTGCCATACTCGATACGGTAAGCACCTTTCCCCTTATTCCCGCCCCGGTGCGGACCATTATTCAGGCCGTGC                    <  SRR4302537.1225142/94‑1 (MQ=60)
                                                                       GACGATTTCGGTGAAACCGTAGGTGCCATACTCGATACGGTAAGCACCTTTCCCCTTATTCCCGCCCCGGTGCGGACCATTATTCAGGCCGTGC                    >  SRR4302537.3923386/1‑94 (MQ=60)
                                                                         CGATTTCGGTGAAACCGTAGGTGCCATACTCGATACGGTAAGCACCTTTCCCCTTATTCCAGCCCCGGTGCGGACCATTATTCAGGCCGTGCGT                  >  SRR4302537.4972901/1‑94 (MQ=60)
                                                                           ATTTCGGTGAAACCGTAGGTGCCATACTCGATACGGTAAGCACCTTTCCCCTTATTCCCGCCCCGGTGCGGACCATTATTCAGGCCGTGCGTTC                >  SRR4302537.5588863/1‑94 (MQ=60)
                                                                           ATTTCGGTGAAACCGTAGGTGCCATACTCGATACGGTAAGCACCTTTCCCCTTATTCCCGCCCCGGTGCGGACCATTATTCAGGCCGTGCGTTC                >  SRR4302537.5042584/1‑94 (MQ=60)
                                                                           ATTTCGGTGAAACCGTAGGTGCCATACTCGATACGGTAAGCACCTTTCCCCTTATTCCCGCCCCGGTGCGGACCATTATTCAGGCCGTGCGTTC                >  SRR4302537.116415/1‑94 (MQ=60)
                                                                           ATTTCGGTGAAACCGTAGGTGCCATACTCGATACGGTAAGCACCTTTCCCCTTATTCCCGCCCCGGTGCGGACCATTATTCAGGCCGTGCGTTC                >  SRR4302537.1049346/1‑94 (MQ=60)
                                                                             TTCGGTGAAACCGTAGGTGCCATACTCGATACGGTAAGCACCTTTCCCCTTATTCCCGCCCCGGTGCGGACCATTATTCAGGCCGTGCGTTCCT              <  SRR4302537.5568333/94‑1 (MQ=60)
                                                                             TTCGGTGAAACCGTAGGTGCCATACTCGATACGGTAAGCACCTTTCCCCTTATCCCCGCCCCGGTGCGGACCATTATTCAGGCCGTGCGTTCCT              <  SRR4302537.4202727/94‑1 (MQ=60)
                                                                              TCGGTGAAACCGTAGGTGCCATACTCGATACGGTAAGCCCCTTTCCCCTTATTCCCGCCCCGGTGCGGACCATTATTCAGGCCGTGCGTTCCTC             <  SRR4302537.4103953/94‑1 (MQ=60)
                                                                              TCGGTGAAACCGTAGGTGCCATACTCGATACGGTAAGCACCTTTCCCCTTATCCCCGCCCCGGTGCGGACCATTATTCAGGCCGTGCGTTCCTC             <  SRR4302537.5557883/94‑1 (MQ=60)
                                                                               CGGTGAAACCGTAGGTGCCATACTCGATACGGTAAGCACCTTTCCCCTTATTCCCGCCCCGGTGCGGACCATTATTCAGGCCGTGCGTTCCTCG            <  SRR4302537.6359675/94‑1 (MQ=60)
                                                                                GGTGAAACCGTAGGTGCCATACTCGATACGGTAAGCACCTTTCCCCTTATTCCCGCCCCGGTGCGGACCATTATTCAGGCCGTGCGTTCCTCGG           <  SRR4302537.1559704/94‑1 (MQ=60)
                                                                                 GTGAAACCGTAGGTGCCATACTCGATACGGTAAGCACCTTTCCCCTTATTCCCGCCCCGGTGCGGACCATTATTCAGGCCGTGCGTTCCTCGGT          >  SRR4302537.2734985/1‑94 (MQ=60)
                                                                                 GTGAAACCGTAGGTGCCATACTCGATACGGTAAGCACCTTTCCCCTTATTCCCGCCCCGGTGCGGACCATTATTCAGGCCGTGCGTTCCTCGGT          <  SRR4302537.2248377/94‑1 (MQ=60)
                                                                                  TGAAACCGTAGGTGCCATACTCGATACGGTAAGCACCTTTCCCCTTATTCCCGCCCCGGTGCGGACCATTATTCAGGCCGTGCGTTCCTCGGTG         >  SRR4302537.3451347/1‑94 (MQ=60)
                                                                                     AACCGTAGGTGCCATACTCGATACGGTAAGCACCTTTCCCCTTATTCCCGCCCCGGTGCGGACCATTATTCAGGCCGTGCGTTCCTCGGTGGTG      >  SRR4302537.1580314/1‑94 (MQ=60)
                                                                                     AACCGTAGGTGCCATACTCGATACGGTAAGCACCTTTCCCCTTATTCCCGCCCCGGTGCGGACCATTATTCAGGCCGTGCGTTCCTCGGTGGTG      >  SRR4302537.4914566/1‑94 (MQ=60)
                                                                                      ACCGTAGGTGCCATACTCGATACGGTAAGCACCTTTCCCCTTATTCCCGCCCCGGTGCGGACCATTATTCAGGCCGTGCGTTCCTCGGTGGTGT     >  SRR4302537.910583/1‑94 (MQ=60)
                                                                                        CGTAGGTGCCATACTCGATACGGTAAGCTCCTTTCCCCTTATTCCCGCCCCGGTGCGGACCATTATTCAGGCCGTGCGTTCCTCGGTGGTGTCA   <  SRR4302537.6420230/94‑1 (MQ=60)
                                                                                        CGTAGGTGCCATACTCGATACGGTAAGCACCTTTCCCCTTATTCCCGCCCCGGTGCGGACCATTATTCAGGCCGTGCGTTCCTCGGTGGTGTCA   >  SRR4302537.1593741/1‑94 (MQ=60)
                                                                                         GTAGGTGCCATACTCGATACGGTAAGCACCTTTCCCCTTATTCCCGCCCCGGTGCGGACCATTATTCAGGCCGTGCGTTCCTCGGTGGTGTCAG  >  SRR4302537.3963336/1‑94 (MQ=60)
                                                                                         GTAGGTGCCATACTCGATACGGTAAGCACCTTTCCCCTTATTCCCGCCCCGGTGCGGACCATTATTCAGGACGTGCGTTCCTCGGTGGTGTCAG  >  SRR4302537.4107099/1‑94 (MQ=60)
                                                                                             |                                                                                         
AGCCCAGCAGCCCGGTAGTAGCGCTACTGCAACATCCCTTTCGGACATGGGTAGCCCGGGAGCAGGCACGTGACGATTTCGGTGAAACCGTAGATACCATACTCGATACGGTAAGCACCTTTCCCCTTATTCCCGCCCCGGTGCGGACCATTATTCAGGCCGTGCGTTCCTCGGTGGTGTCAG  >  NC_000913/2766380‑2766562

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 2 ≤ ATCG/ATCG < 23 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑