Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I2 R1 882 254.4 12957588 98.0% 12698436 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,767,669 (C)6→7 intergenic (+162/‑55) yfjQ → / → yfjR hypothetical protein/hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,767,6631.C95.4% 699.4 / 24.2 217intergenic (+156/‑61)yfjQ/yfjRhypothetical protein/hypothetical protein
Reads supporting (aligned to +/- strand):  ref base . (6/4);  new base C (112/94);  total (118/99)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.53e-01

Only 100 of 247 total aligned reads displayed.

GCCTCAATCCCCCGGTTATAGCTTTTAACCCCCGTTACATCTGGCTTTTGCAGAAATAAAAAATAGTTTCTGCGTTGTCCATACCCTGTCCG‑CCC‑CCCTCTTTAAAGTAATCACATCATTTTCAGTCAGTTAACTTTCCTGGAGAATCTCTCATGACACAGGCAGAACGCCGCCATGAC  >  NC_000913/2767572‑2767750
                                                                                            |                                                                                        
gCCTCAATCCCCCGGTTATAGCTTTTAACCCCCGTTACATCTGGCTTTTGCAGAAATAAAAAATAGTTTCTGCGTTGTCCATACCCTGTCCG‑c                                                                                         >  1:12308/1‑93 (MQ=255)
 ccTCAATCCCCCGGTTATAGCTTTTAACCCCCGTTACATCTGGCTTTTGCAGAAATAAAAAATAGTTTCTGCGTTGTCCATACCCTGTCCG‑cc                                                                                        <  1:1858435/93‑1 (MQ=255)
  cTCAATCCCCCGGTTATAGCTTTTAACCCCCGTTACATCTGGCTTTTGCAGAAATAAAAAATAGTTTCTGCGTTGTCCATACCCTGTCCG‑ccc                                                                                       >  1:2180651/1‑93 (MQ=255)
   tCAATCCCCCGGTTATAGCTTTTAACCCCCGTTACATCTGGCTTTTGCAGAAATAAAAAATAGTTTCTGCGTTGTCCATACCCTGTCCG‑ccc‑c                                                                                     <  1:574559/93‑1 (MQ=255)
   tCAATCCCCCGGTTATAGCTTTTAACCCCCGTTACATCTGGCTTTTGCAGAAATAAAAAATAGTTTCTGCGTTGTCCATACCCTGTCCG‑ccc‑c                                                                                     >  2:6120488/1‑93 (MQ=255)
     aaTCCCCCGGTTATAGCTTTTAACCCCCGTTACATCTGGCTTTTGCAGAAATAAAAAATAGTTTCTGCGTTGTCCATAACCTGTCCG‑ccc‑ccc                                                                                   <  1:5997559/93‑1 (MQ=255)
      aTCCCCCGGTTATAGCTTTTAACCCCCGTTACATCTGGCTTTTGCAGAAATAAAAAATAGTTTCTGCGTTGTCCATACCCTGTCCG‑ccc‑cccc                                                                                  >  1:28436/1‑92 (MQ=255)
      aTCCCCCGGTTATAGCTTTTAACCCCCGTTACATCTGGCTTTTGCAGAAATAAAAAATAGTTTCTGCGTTGTCCATACCCTGTCCG‑ccc‑cccc                                                                                  >  2:5237920/1‑92 (MQ=255)
      aTCCCCCGGTTATAGCTTTTAACCCCCGTTACATCTGGCTTTTGCAGAAATAAAAAATAGTTTCTGCGTTGTCCATACCCTGTCCG‑ccc‑cccc                                                                                  <  1:3402451/93‑2 (MQ=255)
      aTCCCCCGGTTATAGCTTTTAACCCCCGTTACATCTGGCTTTTGCAGAAATAAAAAATAGTTTCTGCGTTGTCCATACCCTGTCCG‑ccc‑cccc                                                                                  >  2:230589/1‑92 (MQ=255)
         ccccGGTTATAGCTTTTAACCCCCGTTACATCTGGCTTTTGCAGAAATAAAAAATAGTTTCTGCGTTGTCCATACCCTGTCCG‑ccc‑cccctct                                                                               <  1:4333027/93‑5 (MQ=255)
         ccccGGTTATAGATTATAACCCCCGTTACATCTGGCTTTTGCAGAAATAAAAAATAGTTTCTGCGTTGTCCATACCCTGTCCG‑ccc‑cccctct                                                                               <  2:2797863/93‑5 (MQ=21)
           ccGGTTATAGCTTTTAACCCCCGTTACATCTGGCTTTTGCAGAAATAAAAAATAGTTTCTGCGTTGTCCATACCCTGTGCGCCCC‑CCCTCttt                                                                              >  2:2578908/1‑93 (MQ=255)
           ccGGTTATAGCTTTTAACCCCCGTTACATCTGGCTTTTGCAGAAATAAAAAATAGTTTCTGCGTTGTCCATACCCTGTCCGCCCC‑CCCTCttt                                                                              <  1:5107066/93‑1 (MQ=255)
           ccGGTTATAGCTTTTAACCCCCGTTACATCTGGCTTTTGCAGAAATAAAAAATAGTTTCTGCGTTGTCCATACCCTGTCCGCCCC‑CCCTCttt                                                                              >  2:491167/1‑93 (MQ=255)
            cGGTTATAGCTTTTAACCCCCGTTACATCTGGCTTTTGCAGAAATAAAAAATAGTTTCTGCGTTGTCCATACCCTGTCCGCCCC‑CCCTCTTTa                                                                             >  2:3781872/1‑93 (MQ=255)
              gTTATAGTCTTTAACCCCTGTTACATCTGGCTTTTGCAGAAATAAAAAATAGTTTCTGCGTTGTCCATACCCTGTCCGCCCC‑CCCTCTTTaaa                                                                           <  1:2339132/93‑1 (MQ=18)
              gTTATAGCTTTTAACCCCCGTTACATCTGGCTTTTGCAGAAATAAAAAATAGTTTCTGCGTTGTCCATACCCTGTCCGCCCC‑CCCTCTTTaaa                                                                           >  1:5615517/1‑93 (MQ=255)
              gTTATAGCTTTTAACCCCCGTTACATCTGGCTTTTGCAGAAATAAAAAATAGTTTCTGCGTTGTCCATACCCTGTCCGCCCC‑CCCTCTTTaaa                                                                           <  1:5541635/93‑1 (MQ=255)
               ttATAGCTTTTAACCCCCGTTACATCTGGCTTTTGCAGAAATAAAAAATAGTTTCTGCGTTGTCCATACCCTGTCCGCCCC‑CCCTCTTTAAAg                                                                          <  2:1000630/93‑1 (MQ=255)
               ttATAGCTTTTAACCCCCGTTACATCTGGCTTTTGCAGAAATAAAAAATAGTTTCTGCGTTGTCCATACCCTGTCCGCCCC‑CCCTCTTTAAAg                                                                          <  2:4665609/93‑1 (MQ=255)
                tataGCTTTTAACCCCCGTTACATCTGGCTTTTGCAGAAATAAAAAATAGTTTCTGCGTTGTCCATACCCTGTCCGCCCC‑CCCTCTTTAAAGt                                                                         <  1:3569749/93‑1 (MQ=255)
                 ataGCTTTTAACCCCCGTTACATCTGGCTTTTGCAGAAATAAAAAATAGTTTCTGCGTTGTCCATACCCTGTCCGCCCC‑CCCTCTTTAAAGTa                                                                        >  1:4883091/1‑93 (MQ=255)
                  taGCTTTTAACCCCCGTTACATCTGGCTTTTGCAGAAATAAAAAATAGTTTCTGCGTTGTCCATACCCTGTCCGCCCC‑CCCTCTTTAAAGTaa                                                                       >  1:1976328/1‑93 (MQ=255)
                    gCTTTTAACCCCCGTTACATCTGGCTTTTGCAGAAATAAAAAATAGTTTCTGCGTTGTCCATACCCTGTCCGCCCC‑CCCTCTTTCAAGTAATc                                                                     >  2:4794105/1‑93 (MQ=255)
                      ttttACCCCCCGTTACATCTGGCTTTTGCAGAAATAAAAAATAGTTTCTGCGTTGTCCATACCCTGTCCGCCCC‑CCCTCTTTAAAGTAATcac                                                                   <  1:165330/93‑1 (MQ=255)
                      ttttAACCCCCGTTACATCTGGCTTTTGCAGAAATAAAAAATAGTTTCTGCGTTGTCCATACCCTGTCCGCCCC‑CCCTCTTTAAAGTAATcac                                                                   >  1:4044061/1‑93 (MQ=255)
                       tttAACCCCCGTTACATCTGGCTTTTGCAGAAATAAAAAATAGTTTCTGCGTTGTCCATACCCTGTCCGCCCC‑CCCTCTTTAAAGTAATcaca                                                                  <  2:1167220/93‑1 (MQ=255)
                         tatcccccGTTACATCTGGCTTTTGCAGAAATAAAAAATAGTTTCTGCGTTGTCCATACCCTGTCCGCCCC‑CCCTCTTTAAAGTAATCAcatc                                                                <  1:2986469/90‑1 (MQ=255)
                         tAACCCCCGTTACATCTGGCTTTTGCAGAAATAAAAAATAGTTTCTGCGTTGTCCATACCCTGTCCGCCCC‑CCCTCTTTAAAGTAATCAcatc                                                                <  2:1294775/93‑1 (MQ=255)
                          aaCCCCCGTTACATCTGGCTTTTGCAGAAATAAAAAATAGTTTCTGCGTTGTCCATACCCTGTCCGCCCC‑CCCTCTTTAAAGTAATCAcatca                                                               >  1:397599/1‑93 (MQ=255)
                          aaCCCCCGTTACATCTGGCTTTTGCAGAAATAAAAAATAGTTTCTGCGTTGTCCATACCCTGTCCGCCCC‑CCCTCTTTAAAGTAATCAcatca                                                               <  2:1505239/93‑1 (MQ=255)
                          aaCCCCCGTTACATCTGGCTTTTGCAGAAATAAAAAATAGTTTCTGCGTTGTCCATACCCTGTCCGCCCC‑CCCTCTTTAAAGTAATCAcatca                                                               >  2:2945671/1‑93 (MQ=255)
                           aCCCCCGTTACATCTGGCTTTTGCAGAAATAAAAAATAGTTTCTGCGTTGTCCATACCCTGTCCGCCCC‑CCCTCTTTAAAGTAATCAcatcat                                                              >  2:2086282/1‑93 (MQ=255)
                            cccccGTTACATCTGGCTTTTGCAGAAATAAAAAATAGTTTCTGCGTTGTCCATACCCTGTCCGCCCC‑CCCTCTTTAAAGTAATCACATCAtt                                                             <  2:4828570/93‑1 (MQ=255)
                             ccccGTTACATCTGGCTTTTGCAGAAATAAAAAATAGTTTCTGCGTTGTCCATACCCTGTCCGCCCC‑CCCTCTTTAAAGTAATCACATCAttt                                                            >  2:785766/1‑93 (MQ=255)
                              cccGTTACATCTGGCTTTTGCAGAAATAAAAAATAGTTTCTGCGTTGTCCATCCCCTGTCCGCCCC‑CCCTCTTTAAAGTAATCACATCAtttt                                                           <  1:2809410/93‑1 (MQ=255)
                               ccGTTACATCTGGCTTTTGCAGAAATAAAAAATAGTTTCTGCGTTGTCCATACCCTGTCCGCCCC‑CCCTCTTTAAAGTAATCACATCATTTtc                                                          >  2:3125724/1‑93 (MQ=255)
                                 gTTACATCTGGCTTTTGCAGAAATAAAAAATAGTTTCTGCGTTGTCCATACCCTGTCCGCCCC‑CCCTCTTTAAAGTAATCACATCATTTtcag                                                        >  2:6258918/1‑93 (MQ=255)
                                  ttACATCTGGCTTTTGCAGAAATAAAAAATAGTTTCTGCGTTGTCCATACCCTGTCCGCCCC‑CCCTCTTTAAAGTAATCACATCATTTtcagt                                                       <  2:5182924/93‑1 (MQ=255)
                                   tACATCTGGCTTTTGCAGAAATAAAAAATAGTTTCTGCGTTGTCCATACCCTGTCCGCCCC‑CCCTCTTTAAAGTAATCACATCATTTtcagtc                                                      <  1:4765443/93‑1 (MQ=255)
                                   tACATCTGGCTTTTGCAGAAATAAAAAATAGTTTCTGCGTTGTCCATACCCTGTCCGCCCC‑CCCTCTTTAAAGTAATCACATCATTTtcagtc                                                      >  1:5313884/1‑93 (MQ=255)
                                   tACATCTGGCTTTTGCAGAAATAAAAAATAGGTTCTGCGTTGTCCATACCCTGTCCG‑CCCACCCTCTTTAAAGTAATCACATCATTTtcagtc                                                      >  2:4362605/1‑93 (MQ=255)
                                    aCATCTGGCTTTTGCAGAAATAAAAAATAGTTTCTGCGTTGTCCATACCCTGTCCGCCCC‑CCCTCTTTAAAGTAATCACATCATTTtcagtca                                                     <  1:3236075/93‑1 (MQ=255)
                                      aTCTGGCTTTTGCAGAAATAAAAAATAGTTTCTGCGTTGTCCATACCCTGTCCGCCCC‑CCCTCTTTAAAGTAATCACATCATTTTcagtcagt                                                   >  1:347392/1‑93 (MQ=255)
                                      aTCTGGCTTTTGCAGAAATAAAAAATAGTTTCTGCGTTGTCCATACCCTGTCCGCCCC‑CCCTCTTTAAAGTAATCACATCATTTTcagtcagt                                                   >  1:451176/1‑93 (MQ=255)
                                      aTCTGGCTTTTGCAGAAATAAAAAATAGTTTCTGCGTTGTCCATACCCTGTCCGCCCC‑CCCTCTTTAAAGTAATCACATCATTTTcagtcagt                                                   >  1:3764885/1‑93 (MQ=255)
                                      aTCTGGCTTTTGCAGAAATAAAAAATAGTTTCTGCGTTGTCCATACCCTGTCCGCCCC‑CCCTCTTTAAAGTAATCACATCATTTTcagtcagt                                                   <  2:5520635/93‑1 (MQ=255)
                                       tCTGGCTTTTGCAGAAATAAAAAATAGTTTCTGCGTTGTCCATACCCTGTCCGCCCC‑CCCTCTTTAAAGTAATCACATCATTTTCAGTCAGtt                                                  >  1:2463803/1‑93 (MQ=255)
                                       tCTGGCTTTTGCAGAAATAAAAAATAGTTTCTGCGTTGTCCATACCCTGTCCGCCCC‑CCCTCTTTAAAGTAATCACATCATTTTCAGTCAGtt                                                  <  2:5159181/93‑1 (MQ=255)
                                       tCTGGCTTTTGCAGAAATAAAAAATAGTTTCTGCGTTGTCCATACCCTGTCCGCCCC‑CCCTCTTTAAAGTAATCACATCATTTTCAGTCAGtt                                                  >  2:4314349/1‑93 (MQ=255)
                                       tCTGGCTTTTGCAGAAATAAAAAATAGTTTCTGCGTTGTCCATACCCTGTCCGCCCC‑CCCTCTTTAAAGTAATCACATCATTTTCAGTCAGtt                                                  <  2:4200931/93‑1 (MQ=255)
                                        cTGGCTTTTGCAGAAATAAAAAATAGTTTCTGCGTTGTCCATACCCTGTCCGCCCC‑CCCTCTTTAAAGTAATCACATCATTTTCAGTCAGTTa                                                 <  1:6124749/93‑1 (MQ=255)
                                         tGGCTTGGGCAGAAATAAAAAATTGTTTCTGCGTTGTCCATACCCTGTCCGCCCC‑CCCTCTGTAAAGTAATCACATCATTTTCAGTCAGCTaa                                                >  1:6469594/1‑93 (MQ=14)
                                            cTTTTGCAGAAATAAAAAATAGTTTCTGCGTTGTCCATACCCTGTCCGCCCC‑CCCTCTTTAAAGTAATCACATCATTTTCAGTCAGTTAACtt                                             >  1:5557928/1‑93 (MQ=255)
                                               ttGCAGAAATAAAAAATAGTTTCTGCGTTGTCCATACCCTGTCCGCCCC‑CCCTCTTTAAAGTAATCACATCATTTTCAGTCAGTTAACTTTcc                                          <  2:6039988/93‑1 (MQ=255)
                                                tGCAGAAATAAAAAATAGTTTCTGCGTTGTCCATACCCTGTCCGCCCC‑CCCTCTTTAAAGTAATCACATCATTTTCAGTCAGTTAACTTTCCt                                         <  1:3694691/93‑1 (MQ=255)
                                                 gCAGAAATAAAAAATAGTTTCTGCGTTGTCCATACCCTGTCCGCCCC‑CCCTCTTTAAAGTAATCACATCATTTTCAGTCAGTTAACTTTCCTg                                        <  2:2567605/93‑1 (MQ=255)
                                                 gCAGAAATAAAAAATAGTTTCTGCGTTGTCCATACCCTGTCCGCCCC‑CCCTCTTTAAAGTAATCACATCATTTTCAGTCAGTTAACTTTCCTg                                        <  2:3706072/93‑1 (MQ=255)
                                                 gCAGAAATAAAAAATAGTTTCTGCGTTGTCCATACCCTGTCCGCCCC‑CCCTCTTTAAAGTAATCACATCATTTTCAGTCAGTTAACTTTCCTg                                        >  2:3272373/1‑93 (MQ=255)
                                                  cAGAAATAAAAAATAGTTTCTGCGTTGTCCATACCCTGTCCGCCCC‑CCCTCTTTAAAGTAATCACATCATTTTCAGTCAGTTAACTTTCCTgg                                       >  2:713534/1‑93 (MQ=255)
                                                  cAGAAATAAAAAATAGTTTCTGCGTTGTCCATACCCTGTCCG‑CCC‑CCCTCTTTAAAGTAATCACATCATTTTCAGTCAGTTAACTTTCCTGga                                      <  2:3464352/93‑1 (MQ=255)
                                                     aaaTAAAAAATAGTTTCTGCGTTGTCCATACCCTGTCCGCCCC‑CCCTCTTTAAAGTAATCACATCATTTTCAGTCAGTTAACTTTCCTGgaga                                    <  2:2383659/93‑1 (MQ=255)
                                                     aaaTAAAAAATAGTTTCTGCGTTGTCCATACCCTGTCCGCCCC‑CCCTCTTTAAAGTAATCACATCATTTTCAGTCAGTTAACTTTCCTGgaga                                    >  2:5908721/1‑93 (MQ=255)
                                                       aTAAAAAATAGTTTCTGCGTTGTCCATCCCCTGTCCGCCCC‑CCCTCTTTAAAGTAATCACATCATTTTCAGTCAGTTAACTTTCCTGGAGAAt                                  <  1:1419966/93‑1 (MQ=255)
                                                       aTAAAAAATAGTTTCTGCGTTGTCCATACCCTGTCCGCCCC‑CCCTCTTTAAAGTAATCACATCATTTTCAGTCAGTTAACTTTCCTGGAGAAt                                  >  1:6052152/1‑93 (MQ=255)
                                                         aaaaaaTAGTTTCTGCGTTGTCCATACCCTGTCCGCCCC‑CCCTCTTTAAAGTAATCACATCATTTTCAGTCAGTTAACTTTCCTGGAGAAtct                                >  2:3794254/1‑93 (MQ=255)
                                                          aaaaaTAGTTTCTGCGTTGTCCATACCCTGTCCGCCCC‑CCCTCTTTAAAGTAATCACATCATTTTCCGTCAGTTAAATTTCCTGTAGAAtctc                               >  2:935285/1‑93 (MQ=17)
                                                          aaaaaTAGTTTCTGCGTTGTCCATACCCTGTCCGCCCC‑CCCTCTTTAAAGTAATCACATCATTTTCAGTCAGTTAACTTTCCTGGAGAAtctc                               <  2:1431361/93‑1 (MQ=255)
                                                          aaaaaTAGTTTCTGCGTTGTCCATACCCTGTCCGCCCC‑CCCTCTTTAAAGTAATCACATCATTTTCAGTCAGTTAACTTTCCTGGAGAAtctc                               >  2:707417/1‑93 (MQ=255)
                                                          aaaaaTAGTTTCTGCGTTGTCCATACCCTGTCCGCCCC‑CCCTCTTTAAAGTAATCACATCATTTTCAGTCAGTTAACTTTCCTGGAGAAtctc                               <  1:1745291/93‑1 (MQ=255)
                                                          aaaaaTAGTTTCTGCGTTGTCCATACCCTGTCCGCCCC‑CCCTCTTTAAAGTAATCACATCATTTTCAGTCAGTTAACTTTCCTGGAGAAtctc                               <  1:4199568/93‑1 (MQ=255)
                                                           aaaaTAGTTTCTGCGTTGTCCATACCCTGTCCGCCCC‑CCCTCTTTAAAGTAATCACATCATTTTCAGTCAGTTAACTTTCCTGGAGAAtctct                              >  2:550758/1‑93 (MQ=255)
                                                            aaaTAGTTTCTGCGTTGTCCATACCCTGTCCGCCCC‑CCCTCTTTAAAGTAATCACATCATTTTCAGTCAGTTAACTTTCCTGGAGAAtctctc                             >  1:4146513/1‑93 (MQ=255)
                                                            aaaTAGTTTCTGCGTTGTCCATACCCTGTCCGCCCC‑CCCTCTTTAAAGTAATCACATCATTTTCAGTCAGTTAACTTTCCTGGAGAAtctctc                             >  2:2836211/1‑93 (MQ=255)
                                                            aaaTAGTTTCTGCGTTGTCCATACCCTGTCCGCCCC‑CCCTCTTTAAAGTAATCACATCATTTTCAGTCAGTTAACTTTCCTGGAGAAtctctc                             <  1:4628842/93‑1 (MQ=255)
                                                            aaaTAGTTTCTGCGTTGTCCATACCCTGTACGCCCC‑CCCTCTTTAAAGTAATCACATCATTTTCAGTCAGTTAACTTTCCTGGAGGAtctctc                             >  2:6374878/1‑93 (MQ=17)
                                                               tAGTTTCTGCGTTGTCCATACCCTGTCCGCCCC‑CCCTCTTTAAAGTAATCACATCATTTTCAGTCAGTTAACTTTCCTGGAGAATCTCTCATg                          <  2:1055461/93‑1 (MQ=255)
                                                                    tCTGCGTTGTCCATACCCTGTCCGCCCC‑CCCTCTTTAAAGTAATCACATCATTTTCAGTCAGTTAACTTTCCTGGAGAATCTCTCATGAcaca                     <  1:1524501/93‑1 (MQ=255)
                                                                    tCTGCGTTGTCCATACCCTGTCCGCCCC‑CCCTCTTTAAAGTAATCACATCATTTTCAGTCAGTTAACTTTCCTGGAGAATCTCTCATGAcaca                     <  1:702979/93‑1 (MQ=255)
                                                                     cTGCGTTGTCCATACCCTGTCCGCCCC‑CCCTCTTTAAAGTAATCACATCATTTTCAGTCAGTTAACTTTCCTGGAGAATCTCTCATGACACAg                    >  2:3361568/1‑93 (MQ=255)
                                                                     cTGCGTTGTCCATACCCTGTCCGCCCC‑CCCTCTTTAAAGTAATCACATCATTTTCAGTCAGTTAACTTTCCTGGAGAATCTCTCATGACACAg                    <  1:4171709/93‑1 (MQ=255)
                                                                     cTGCGTTGTCCATACCCTGTCCGCCCC‑CCCTCTTTAAAGTAATCACATCATTTTCAGTCAGTTAACTTTCCTGGAGAATCTCTCATGACACAg                    <  2:6209987/93‑1 (MQ=255)
                                                                     cTGCGTTGTCCATACCCTGTCCGCCCC‑CCCTCTTTAAAGTAATCACATCATTTTCAGTCAGTTAACTTTCCTGGAGAATCTCTCATGACACAg                    <  1:954447/93‑1 (MQ=255)
                                                                      tGCGTTGTCCATACCCTGTCCGCCCC‑CCCTCTTTAAAGTAATCACATCATTTTCAGTCAGTTAACTTTCCTGGAGAATCTCTCATGACACAgg                   >  1:6338491/1‑93 (MQ=255)
                                                                       gCGTTGTCCATACCCTGTCCGCCCC‑CCCTCTTTAAAGGAATCACATCATTTTCAGTCAGTTAACTTTCCTGGAGAATCTCTCATCACACAGGc                  >  2:5451254/1‑93 (MQ=17)
                                                                        cGTTGTCCATACCCTGTCCGCCCC‑CCCTCTTTAAAGTAATCACATCATTTTCAGTCAGTTAACTTTCCTGGAGAATCTCTCATGACACAGGCa                 <  2:102457/93‑1 (MQ=255)
                                                                          ttGTCCATACCCTGTCCGCCCC‑CCCTCTTTAAAGTAATCACATCATTTTCAGTCAGTTAACTTTCCTGGAGAATCTCTCATGACACAGGCAGa               <  2:1209953/93‑1 (MQ=255)
                                                                           tGTCCATACCCTGTCCGCCCC‑CCCTCTTTAAAGTAATCACATCATTTTCAGTCAGTTAACTTTCCTGGAGAATCTCTCATGACACAGGCAGaa              <  1:1053399/93‑1 (MQ=255)
                                                                           tGTCCATACCCTGTCCGCCCC‑CCCTCTTTAAAGTAATCACATCATTTTCAGTCAGTTAACTTTCCTGGAGAATCTCTCATAACACAGGCAGaa              <  1:1344806/93‑1 (MQ=17)
                                                                            gTCCATACCCTGTCCGCCCC‑CCCTCTTTAAAGTAATCACATCATTTTCAGTCAGTTAACTTTCCTGGAGAATCTCTCATGACACAGGCAGAAc             <  1:3258670/93‑1 (MQ=255)
                                                                             tCCATACCCTGTCCG‑CCC‑CCCTCTTTAAAGTAATCACATCATTTTCAGTCAGTTAACTTTCCTGGAGAATCTCTCATGACACAGGCAGAAcgc           >  1:2622875/1‑93 (MQ=255)
                                                                               cATACCCTGTCCGCCCC‑CCCTCTTTAAAGTGATCACATCATTTTCAGTCAGTTAACTTTCCTGGAGAATCTCTCATGACACAGGCAGAAcgcc          <  2:4124690/93‑1 (MQ=255)
                                                                               cATACCCTGTCCGCCCC‑CCCTCTTTAAAGTAATCACATCATTTTCAGTCAGTTAACTTTCCTGGAGAATCTCTCATGACACAGGCAGAAcgcc          >  2:3547886/1‑93 (MQ=255)
                                                                                aTACCCTGTCCGCCCC‑CCCTCTTTAAAGTAATCACATCATTTTCAGTCAGTTAACTTTCCTGGAGAATCTCTCATGACACAGGCAGAAcgccg         >  1:3128122/1‑93 (MQ=255)
                                                                                 tACCCTGTCCGCCCC‑CCCTCTTTAAAGTAATCACATCATTTTCAGTCAGTTAACTTTCCTAGAGAATCTCTCATGACACAGGCAGAAcgccgc        >  2:5779934/1‑93 (MQ=255)
                                                                                  aCCCTGTCCGCCCC‑CCCTCTTTAAAGTAATCACATCATTTTCAGTCAGTTAACTTTCCTGGAGAATCTCTCATGACACAGGCAGAACgccgcc       >  2:2341213/1‑93 (MQ=255)
                                                                                    ccTGTCCGCCCC‑CCCTCTTTAAAGTAATCACATCATTTTCAGTCAGTTAACTTTCCTGGAGAATCTCTCATGACACAGGCAGAACGCCGCCAt     <  2:4158651/93‑1 (MQ=255)
                                                                                      tGTCCGCCCC‑CCCTCTTTAAAGTAATCACATCATTTTCAGTCAGTTAACTTTCCTGGAGAATCTCTCATGACACAGGCAGAACGCCGCCATGa   >  2:4067206/1‑93 (MQ=255)
                                                                                      cgTCCGCCCC‑CCCTCTTTAAAGTAATCACATCATTTTCAGTCAGTTAACTTTCCTGGAGAATCTCTCATGACACAGGCAGAACGCCGCCATGa   <  1:4845658/92‑1 (MQ=255)
                                                                                            |                                                                                        
GCCTCAATCCCCCGGTTATAGCTTTTAACCCCCGTTACATCTGGCTTTTGCAGAAATAAAAAATAGTTTCTGCGTTGTCCATACCCTGTCCG‑CCC‑CCCTCTTTAAAGTAATCACATCATTTTCAGTCAGTTAACTTTCCTGGAGAATCTCTCATGACACAGGCAGAACGCCGCCATGAC  >  NC_000913/2767572‑2767750

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 18 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
AATCCCCCGGTTATAGCTTTTAACCCCCGTTACATCTGGCTTTTGCAGAAATAAAAAATAGTTTCTGCGTTGTCCATACCCTGTCCG‑CCCCCCTCTTTAAAGTAATCACATCATTTTCAGTCAGTTAACTTTCCTGGAGAATCTCTCATGACACAGGCAGAACGCCGCCATGACCGGCTGGCTGTC  >  NC_000913/2767577‑2767762
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AATCCCCCGGTTATAGCTTTTAACCCCCGTTACATCTGGCTTTTGCAGAAATAAAAAATAGTTTCTGCGTTGTCCATACCCTGTCCG‑CCCCCCC                                                                                              >  SRR4302537.3432263/1‑94 (MQ=60)
AATCCCCCGGTTATAGCTTTTAACCCCCGTTACATCTGGCTTTTGCAGAAATAAAAAATAGTTTCTGCGTTGTCCATACCCTGTCCG‑CCCCCCC                                                                                              >  SRR4302537.28663/1‑94 (MQ=60)
AATCCCCCGGTTATAGCTTTTAACCCCCGTTACATCTGGCTTTTGCAGAAATAAAAAATAGTTTCTGCGTTGTCCATAACCTGTCCG‑CCCCCCC                                                                                              <  SRR4302537.6038758/94‑1 (MQ=60)
 ATCCCCCGGTTATAGCTTTTAACCCCCGTTACATCTGGCTTTTGCAGAAATAAAAAATAGTTTCTGCGTTGTCCATACCCTGTCCG‑CCCCCCct                                                                                             <  SRR4302537.3426553/94‑3 (MQ=60)
    CCCCGGTTATAGCTTTTAACCCCCGTTACATCTGGCTTTTGCAGAAATAAAAAATAGTTTCTGCGTTGTCCATACCCTGTCCGCCCCCCCTCTT                                                                                           >  SRR4302537.5270994/1‑94 (MQ=60)
    CCCCGGTTATAGCTTTTAACCCCCGTTACATCTGGCTTTTGCAGAAATAAAAAATAGTTTCTGCGTTGTCCATACCCTGTCCGCCCCCCCTCTT                                                                                           <  SRR4302537.4363124/94‑1 (MQ=60)
     CCCGGTTATAGCTTTTAACCCCCGTTACATCTGGCTTTTGCAGAAATAAAAAATAGTTTCTGCGTTGTCCATACCCTGTCCGCCCCCCCTCTTT                                                                                          <  SRR4302537.1155282/94‑1 (MQ=60)
     CCCGGTTATAGCTTTTAACCCCCGTTACATCTGGCTTTTGCAGAAATAAAAAATAGTTTCTGCGTTGTCCATACCCTGTCCGCCCCCCCTCTTT                                                                                          <  SRR4302537.1991142/94‑1 (MQ=60)
      CCGGTTATAGCTTTTAACCCCCGTTACATCTGGCTTTTGCAGAAATAAAAAATAGTTTCTGCGTTGTCCATACCCTGTCCGCCCCCCCTCTTTA                                                                                         <  SRR4302537.5142258/94‑1 (MQ=60)
      CCGGTTATAGCTTTTAACCCCCGTTACATCTGGCTTTTGCAGAAATAAAAAATAGTTTCTGCGTTGTCCATACCCTGTCCGCCCCCCCTCTTTA                                                                                         >  SRR4302537.4184335/1‑94 (MQ=60)
      CCGGTTATAGCTTTTAACCCCCGTTACATCTGGCTTTTGCAGAAATAAAAAATAGTTTCTGCGTTGTCCATACCCTGTCCGCCCCCCCTCTTTA                                                                                         >  SRR4302537.5591184/1‑94 (MQ=60)
       CGGTTATAGCTTTTAACCCCCGTTACATCTGGCTTTTGCAGAAATAAAAAATAGTTTCTGCGTTGTCCATACCCTGTCCGCCCCCCCTCTTTAA                                                                                        >  SRR4302537.3010863/1‑94 (MQ=60)
        GGTTATAGCTTTTAACCCCCGTTACATCTGGCTTTTGCAGAAATAAAAAATAGTTTCTGCGTTGTCCATACCCTGTCCGCCCCCCCTCTTTAAA                                                                                       >  SRR4302537.5653913/1‑94 (MQ=60)
        GGTTATAGCTTTTAACCCCCGTTACATCTGGCTTTTGCAGAAATAAAAAATAGTTTCTGCGTTGTCCATACCCTGTCCGCCCCCCCTCTTTAAA                                                                                       >  SRR4302537.4106814/1‑94 (MQ=60)
         GTTATAGTCTTTAACCCCTGTTACATCTGGCTTTTGCAGAAATAAAAAATAGTTTCTGCGTTGTCCATACCCTGTCCGCCCCCCCTCTTTAAAG                                                                                      <  SRR4302537.2355618/94‑1 (MQ=60)
         GTTATAGCTTTTAACCCCCGTTACATCTGGCTTTTGCAGAAATAAAAAATAGTTTCTGCGTTGTCCATACCCTGTCCGCCCCCCCTCTTTAAAG                                                                                      <  SRR4302537.5579474/94‑1 (MQ=60)
         GTTATAGCTTTTAACCCCCGTTACATCTGGCTTTTGCAGAAATAAAAAATAGTTTCTGCGTTGTCCATACCCTGTCCGCCCCCCCTCTTTAAAG                                                                                      <  SRR4302537.5623539/94‑1 (MQ=60)
         GTTATAGCTTTTAACCCCCGTTACATCTGGCTTTTGCAGAAATAAAAAATAGTTTCTGCGTTGTCCATACCCTGTCCGCCCCCCCTCTTTAAAG                                                                                      <  SRR4302537.4503970/94‑1 (MQ=60)
          TTATAGCTTTTAACCCCCGTTACATCTGGCTTTTGCAGAAATAAAAAATAGTTTCTGCGTTGTCCATACCCTGTCCGCCCCCCCTCTTTAAAGT                                                                                     >  SRR4302537.2479715/1‑94 (MQ=60)
           TATAGCTTTTAACCCCCGTTACATCTGGCTTTTGCAGAAATAAAAAATAGTTTCTGCGTTGTCCATACCCTGTCCGCCCCCCCTCTTTAAAGTA                                                                                    >  SRR4302537.4916818/1‑94 (MQ=60)
           TATAGCTTTTAACCCCCGTTACATCTGGCTTTTGCAGAAATAAAAAATAGTTTCTGCGTTGTCCATACCCTGTCCGCCCCCCCTCTTTAAAGTA                                                                                    <  SRR4302537.3594921/94‑1 (MQ=60)
            ATAGCTTTTAACCCCCGTTACATCTGGCTTTTGCAGAAATAAAAAATAGTTTCTGCGTTGTCCATACCCTGTCCGCCCCCCCTCTTTAAAGTAA                                                                                   >  SRR4302537.1990062/1‑94 (MQ=60)
              AGCTTTTAACCCCCGTTACATCTGGCTTTTGCAGAAATAAAAAATAGTTTCTGCGTTGTCCATACCCTGTCCGCCCCCCCTCTTTAAAGTAATC                                                                                 <  SRR4302537.4843312/94‑1 (MQ=60)
              AGCTTTTAACCCCCGTTACATCTGGCTTTTGCAGAAATAAAAAATAGTTTCTGCGTTGTCCATACCCTGTCCGCCCCCCCTCTTTAAAGTAATC                                                                                 >  SRR4302537.3449609/1‑94 (MQ=60)
                CTTTTAACCCCCGTTACATCTGGCTTTTGCAGAAATAAAAAATAGTTTCTGCGTTGTCCATACCCTGTCCGCCCCCCCTCTTTAAAGTAATCAC                                                                               >  SRR4302537.4072341/1‑94 (MQ=60)
                 TTTTACCCCCCGTTACATCTGGCTTTTGCAGAAATAAAAAATAGTTTCTGCGTTGTCCATACCCTGTCCGCCCCCCCTCTTTAAAGTAATCACA                                                                              <  SRR4302537.166509/94‑1 (MQ=60)
                 TTTTAACCCCCGTTACATCTGGCTTTTGCAGAAATAAAAAATAGTTTCTGCGTTGTCCATACCCTGTCCGCCCCCCCTCTTTAAAGTAATCACA                                                                              >  SRR4302537.2137932/1‑94 (MQ=60)
                 TTTTAACCCCCGTTACATCTGGCTTTTGCAGAAATAAAAAATAGTTTCTGCGTTGTCCATACCCTGTCCGCCCCCCCTCTTTAAAGTAATCACA                                                                              <  SRR4302537.3275983/94‑1 (MQ=60)
                    TATCCCCCGTTACATCTGGCTTTTGCAGAAATAAAAAATAGTTTCTGCGTTGTCCATACCCTGTCCGCCCCCCCTCTTTAAAGTAATCACATCA                                                                           <  SRR4302537.3007336/94‑1 (MQ=60)
                    TAACCCCCGTTACATCTGGCTTTTGCAGAAATAAAAAATAGTTTCTGCGTTGTCCATACCCTGTCCGCCCCCCCTCTTTAAAGTAATCACATCA                                                                           >  SRR4302537.400356/1‑94 (MQ=60)
                       CCCCCGTTACATCTGGCTTTTGCAGAAATAAAAAATAGTTTCTGCGTTGTCCATACCCTGTCCGCCCCCCCTCTTTAAAGTAATCACATCATTT                                                                        <  SRR4302537.4209802/94‑1 (MQ=60)
                       CCCCCGTTACATCTGGCTTTTGCAGAAATAAAAAATAGTTTCTGCGTTGTCCATACCCTGTCCGCCCCCCCTCTTTAAAGTAATCACATCATTT                                                                        <  SRR4302537.803486/94‑1 (MQ=60)
                       CCCCCGTTACATCTGGCTTTTGCAGAAATAAAAAATAGTTTCTGCGTTGTCCATACCCTGTCCGCCCCCCCTCTTTAAAGTAATCACATCATTT                                                                        <  SRR4302537.1384169/94‑1 (MQ=60)
                        CCCCGTTACATCTGGCTTTTGCAGAAATAAAAAATAGTTTCTGCGTTGTCCATACCCTGTCCGCCCCCCCTCTTTAAAGTAATCACATCATTTT                                                                       >  SRR4302537.6082237/1‑94 (MQ=60)
                         CCCGTTACATCTGGCTTTTGCAGAAATAAAAAATAGTTTCTGCGTTGTCCATCCCCTGTCCGCCCCCCCTCTTTAAAGTAATCACATCATTTTC                                                                      <  SRR4302537.2829050/94‑1 (MQ=60)
                         CCCGTTACATCTGGCTTTTGCAGAAATAAAAAATAGTTTCTGCGTTGTCCATACCCTGTCCGCCCCCCCTCTTTAAAGTAATCACATCATTTTC                                                                      >  SRR4302537.5555808/1‑94 (MQ=60)
                          CCGTTACATCTGGCTTTTGCAGAAATAAAAAATAGTTTCTGCGTTGTCCATACCCTGTCCGCCCCCCCTCTTTAAAGTAATCACATCATTTTCA                                                                     >  SRR4302537.4203989/1‑94 (MQ=60)
                           CGTTACATCTGGCTTTTGCAGAAATAAAAAATAGTTTCTGCGTTGTCCATACCCTGTCCGCCCCCCCTCTTTAAAGTAATCACATCATTTTCAG                                                                    <  SRR4302537.4676077/94‑1 (MQ=60)
                             TTACATCTGGCTTTTGCAGAAATAAAAAATAGTTTCTGCGTTGTCCATACCCTGTCCGCCCCCCCTCTTTAAAGTAATCACATCATTTTCAGTC                                                                  >  SRR4302537.5350379/1‑94 (MQ=60)
                              TACATCTGGCTTTTGCAGAAATAAAAAATAGTTTCTGCGTTGTCCATACCCTGTCCGCCCCCCCTCTTTAAAGTAATCACATCATTTTCAGTCA                                                                 <  SRR4302537.4798352/94‑1 (MQ=59)
                               ACATCTGGCTTTTGCAGAAATAAAAAATAGTTTCTGCGTTGTCCATACCCTGTCCGCCCCCCCTCTTTAAAGTAATCACATCATTTTCAGTCAG                                                                <  SRR4302537.2844814/94‑1 (MQ=56)
                               ACATCTGGCTTTTGCAGAAATAAAAAATAGTTTCTGCGTTGTCCATACCCTGTCCGCCCCCCCTCTTTAAAGTAATCACATCATTTTCAGTCAG                                                                <  SRR4302537.3684242/94‑1 (MQ=56)
                               ACATCTGGCTTTTGCAGAAATAAAAAATAGTTTCTGCGTTGTCCATACCCTGTCCGCCCCCCCTCTTTAAAGTAATCACATCATTTTCAGTCAG                                                                <  SRR4302537.3258885/94‑1 (MQ=56)
                                CATCTGGCTTTTGCAGAAATAAAAAATAGTTTCTGCGTTGTCCATACCCTGTCCGCCCCCCCTCTTTAAAGTAATCACATCATTTTCAGTCAGT                                                               >  SRR4302537.3791319/1‑94 (MQ=54)
                                CATCTGGCTTTTGCAGAAATAAAAAATAGTTTCTGCGTTGTCCATACCCTGTCCGCCCCCCCTCTTTAAAGTAATCACATCATTTTCAGTCAGT                                                               >  SRR4302537.4630737/1‑94 (MQ=54)
                                CATCTGGCTTTTGCAGAAATAAAAAATAGTTTCTGCGTTGTCCATACCCTGTCCGCCCCCCCTCTTTAAAGTAATCACATCATTTTCAGTCAGT                                                               >  SRR4302537.454299/1‑94 (MQ=54)
                                CATCTGGCTTTTGCAGAAATAAAAAATAGTTTCTGCGTTGTCCATACCCTGTCCGCCCCCCCTCTTTAAAGTAATCACATCATTTTCAGTCAGT                                                               >  SRR4302537.349806/1‑94 (MQ=54)
                                 ATCTGGCTTTTGCAGAAATAAAAAATAGTTTCTGCGTTGTCCATACCCTGTCCGCCCCCCCTCTTTAAAGTAATCACATCATTTTCAGTCAGTT                                                              >  SRR4302537.2481126/1‑94 (MQ=51)
                                 ATCTGGCTTTTGCAGAAATAAAAAATAGTTTCTGCGTTGTCCATACCCTGTCCG‑CCCCCCTCTTTAAAGTAATCACATCATTTTCAGTCAGTTA                                                             <  SRR4302537.508211/94‑1 (MQ=55)
                                   CTGGCTTTTGCAGAAATAAAAAATAGTTTCTGCGTTGTCCATACCCTGTCCGCCCCCCCTCTTTAAAGTAATCACATCATTTTCAGTCAGTTAA                                                            <  SRR4302537.2293963/94‑1 (MQ=49)
                                   CTGGCTTTTGCAGAAATAAAAAATAGTTTCTGCGTTGTCCATACCCTGTCCGCCCCCCCTCTTTAAAGTAATCACATCATTTTCAGTCAGTTAA                                                            <  SRR4302537.6166849/94‑1 (MQ=49)
                                   CTGGCTTGGGCAGAAATAAAAAATTGTTTCTGCGTTGTCCATACCCTGTCCGCCCCCCCTCTGTAAAGTAATCACATCATTTTCAGTCAGCTAA                                                            >  SRR4302537.6513444/1‑94 (MQ=40)
                                    TGGCTTTTGCAGAAATAAAAAATAGTTTCTGCGTTGTCCATACCCTGTCCGCCCCCCCTCTTTAAAGTAATCACATCATTTTCAGTCAGTTAAC                                                           >  SRR4302537.6131338/1‑94 (MQ=49)
                                     GGCTTTTGCAGAAATAAAAAATAGTTTCTGCGTTGTCCATACCCTGTCCGCCCCCCCTCTTTAAAGTAATCACATCATTTTCAGTCAGTTAACT                                                          >  SRR4302537.5688802/1‑94 (MQ=49)
                                     GGCTTTTGCAGAAATAAAAAATAGTTTCTGCGTTGTCCATACCCTGTCCGCCCCCCCTCTTTAAAGTAATCACATCATTTTCAGTCAGTTAACT                                                          >  SRR4302537.5866940/1‑94 (MQ=49)
                                      GCTTTTGCAGAAATAAAAAATAGTTTCTGCGTTGTCCATACCCTGTCCGCCCCCCCTCTTTAAAGTAATCACATCATTTTCAGTCAGTTAACTT                                                         >  SRR4302537.5595902/1‑94 (MQ=49)
                                        TTTTGCAGAAATAAAAAATAGTTTCTGCGTTGTCCATACCCTGTCCGCCCCCCCTCTTTAAAGTAATCACATCATTTTCAGTCAGTTAACTTTC                                                       >  SRR4302537.3359184/1‑94 (MQ=49)
                                        TTTTGCAGAAATAAAAAATAGTTTCTGCGTTGTCCATACCCTGTCCGCCCCCCCTCTTTAAAGTAATCACATCATTTTCAGTCAGTTAACTTTC                                                       <  SRR4302537.4770759/94‑1 (MQ=49)
                                        TTTTGCAGAAATAAAAAATAGGTTCTGCGTTGTCCATACCCTGTCCGCCCCCCCTCTTTAAAGTAATCACATCATTTTCAGTCAGTTAACTTTC                                                       >  SRR4302537.6521581/1‑94 (MQ=47)
                                          TTGCAGAAATAAAAAATAGTTTCTGCGTTGTCCATACCCTGTCCGCCCCCCCTCTTTAAAGTAATCACATCATTTTCAGTCAGTTAACTTTCCT                                                     <  SRR4302537.1166456/94‑1 (MQ=54)
                                          TTGCAGAAATAAAAAATAGTTTCTGCGTTGTCCATACCCTGTCCGCCCCCCCTCTTTAAAGTAATCACATCATTTTCAGTCAGTTAACTTTCCT                                                     >  SRR4302537.6477245/1‑94 (MQ=54)
                                           TGCAGAAATAAAAAATAGTTTCTGCGTTGTCCATACCCTGTCCGCCCCCCCTCTTTAAAGTAATCACATCATTTTCAGTCAGTTAACTTTCCTG                                                    <  SRR4302537.3720692/94‑1 (MQ=56)
                                            GCAGAAATAAAAAATAGTTTCTGCGTTGTCCATACCCTGTCCGCCCCCCCTCTTTAAAGTAATCACATCATTTTCAGTCAGTTAACTTTCCTGG                                                   <  SRR4302537.6451554/94‑1 (MQ=59)
                                              AGAAATAAAAAATAGTTTCTGCGTTGTCCATACCCTGTCCGCCCCCCCTCTTTAAAGTAATCACATCATTTTCAGTCAGTTAACTTTCCTGGAG                                                 >  SRR4302537.4338160/1‑94 (MQ=60)
                                              AGAAATAAAAAATAGTTTCTGCGTTGTCCATACCCTGTCCGCCCCCCCTCTTTAAAGTAATCACATCATTTTCAGTCAGTTAACTTTCCTGGAG                                                 >  SRR4302537.4136820/1‑94 (MQ=60)
                                               GAAATAAAAAATAGTTTCTGCGTTGTCCATACCCTGTCCGCCCCCCCTCTTTAAAGTAATCACATCATTTACAGTCAGTTAACTTTCCTGGAGA                                                <  SRR4302537.1447906/94‑1 (MQ=60)
                                                AAATAAAAAATAGTTTCTGCGTTGTCCATACCCTGTCCGCCCCCCCTCTTTAAAGTAATCACATCATTTTCAGTCAGTTAACTTTCCTGGAGAA                                               >  SRR4302537.1740720/1‑94 (MQ=60)
                                                AAATAAAAAATAGTTTCTGCGTTGTCCATACCCTGTCCGCCCCCCCTCTTTAAAGTAATCACATCATTTTCAGTCAGTTAACTTTCCTGGAGAA                                               >  SRR4302537.6113152/1‑94 (MQ=60)
                                                 AATAAAAAATAGTTTCTGCGTTGTCCATACCCTGTCCGCCCCCCCTCTTTAAAGTAATCACATCATTTTCAGTCAGTTAACTTTCCTGGAGAAT                                              >  SRR4302537.6093724/1‑94 (MQ=60)
                                                  ATAAAAAATATTTTCTGCGTTGTCCATACCCTGTCCGCCCCCCCTCTTTAAAGTAATCACATCATTTTCAGTCAGTTAACTTTCCTGGAGAATC                                             <  SRR4302537.3936841/94‑1 (MQ=60)
                                                  ATAAAAAATAGTTTCTGCGTTGTCCATGCCCTGTCCGCCCCCCCTCTTTAAAGTAATCACATCATTTTCAGTCAGTTAACTTTCCTGGAGAATC                                             <  SRR4302537.251733/94‑1 (MQ=60)
                                                  ATAAAAAATAGTTTCTGCGTTGTCCATCCCCTGTCCGCCCCCCCTCTTTAAAGTAATCACATCATTTTCAGTCAGTTAACTTTCCTGGAGAATC                                             <  SRR4302537.1429847/94‑1 (MQ=60)
                                                     AAAAATAGTTTCTGCGTTGTCCATACCCTGTCCGCCCCCCCTCTTTAAAGTAATCACATCATTTTCAGTCAGTTAACTTTCCTGGAGAATCTCT                                          <  SRR4302537.1757460/94‑1 (MQ=60)
                                                     AAAAATAGTTTCTGCGTTGTCCATACCCTGTCCGCCCCCCCTCTTTAAAGTAATCACATCATTTTCAGTCAGTTAACTTTCCTGGAGAATCTCT                                          <  SRR4302537.4228891/94‑1 (MQ=60)
                                                     AAAAATAGTTTCTGCGTTGTCCATACCCTGTCCGCCCCCCCTCTTTAAAGTAATCACATCATTTTCAGTCAGTTAACTTTCCTGGAGAATCTCT                                          >  SRR4302537.298934/1‑94 (MQ=60)
                                                      AAAATAGTTTCTGCGTTGTCCATACCCTGTCCGCCCCCCCTCTTTAAAGTAATCACATCATTTTCAGTCAGTTAACTTTCCTGGAGAATCTCTC                                         >  SRR4302537.4175448/1‑94 (MQ=60)
                                                       AAATAGTTTCTTCGTTGTCCATACCCTGTCCGCCCCCCCTCTTTAAAGTAATCACATCATTTTCAGTCAGTTAACTTTCCTGGAGAATCTCACA                                        <  SRR4302537.1566011/94‑1 (MQ=60)
                                                       AAATAGTTTCTGCGTTGTCCATACCCTGTCCGCCCCCCCTCTTTAAAGTAATCACATCATTTTCAGTCAGTTAACTTTCCTGGAGAATCTCTCA                                        <  SRR4302537.4660868/94‑1 (MQ=60)
                                                         ATAGTTTCTGCGTTGTCCATACCCTGTCCGCCCCCCCTCTTTAAAGTAATCACATCATTTTCAGTCAGTTAACTTTCCTGGAGAATCTCTCATG                                      >  SRR4302537.4416841/1‑94 (MQ=60)
                                                         ATAGTTTCTGCGTTGTCCATACCCTGTCCGCCCCCCCTCTTTAAAGTAATCACATCATTTTCAGTCAGTTAACTTTCCTGGAGAATCTCTCATG                                      >  SRR4302537.5810535/1‑94 (MQ=60)
                                                         ATAGTTTCTGCGTTGTCCATACCCTGTCCGCCCCCCCTCTTTAAAGTAATCACATCATTTTCAGTCAGTTAACTTTCCTGGAGAATCTCTCATG                                      >  SRR4302537.4910201/1‑94 (MQ=60)
                                                          TAGTTTCTGCGTTGTCCATACCCTGTCCGCCCCCCCTCTTTAAAGTAATCACATCATTTTCAGTCAGTTAACTTTCCTGGAGAATCTCTCATGA                                     <  SRR4302537.3764227/94‑1 (MQ=60)
                                                           AGTTTCTGCGTTGTCCATACCCTGTCCGCCCCCCCTCTTTAAAGTAATCACATCATTTTCAGTCAGTTAACTTTCCTGGAGAATCTCTCATGAC                                    >  SRR4302537.3159921/1‑94 (MQ=60)
                                                            GTTTCTGCGTTGTCCATCCCCTGTCCGCCCCCCCTCTTTAAAGTAATCACATCATTTTCAGTCAGTTAACTTTCCTGGAGAATCTCTCATGACA                                   <  SRR4302537.5636015/94‑1 (MQ=60)
                                                            GTTTCTGCGTTGTCCATACCCTGTCCGCCCCCCCTCTTTAAAGTAATCACATCATTTTCAGTCAGTTAACTTTCCTGGAGAATCTCTCATGACA                                   >  SRR4302537.5334810/1‑94 (MQ=60)
                                                             GTTCTGCGTTGTCCATCCCCTGTCCG‑CCCCCCTCTTTAAAGTAATCACATCATTTTCAGTCAGTTAACTTTCCTGGAGAATCTCTCATGACACA                                 <  SRR4302537.3707952/94‑1 (MQ=60)
                                                               TCTGCGTTGTCCATACCCTGTCCGCCCCCCCTCTTTAAAGTAATCACATCATTTTCAGTCAGTTAACTTTCCTGGAGAATCTCTCATGACACAG                                <  SRR4302537.707791/94‑1 (MQ=60)
                                                               TCTGCGTTGTCCATACCCTGTCCGCCCCCCCTCTTTAAAGTAATCACATCATTTTCAGTCAGTTAACTTTCCTGGAGAATCTCTCATGACACAG                                <  SRR4302537.1535149/94‑1 (MQ=60)
                                                                CTGCGTTGTCCATACCCTGTCCGCCCCCCCTCTTTAAAGTAATCACATCATTTTCAGTCAGTTAACTTTCCTGGAGAATCTCTCATGACACAGG                               <  SRR4302537.4200844/94‑1 (MQ=60)
                                                                CTGCGTTGTCCATACCCTGTCCGCCCCCCCTCTTTAAAGTAATCACATCATTTTCAGTCAGTTAACTTTCCTGGAGAATCTCTCATGACACAGG                               <  SRR4302537.961034/94‑1 (MQ=60)
                                                                CTGCGTTGTCCATACCCTGTCCGCCCCCCCTCTTTAAAGTAATCACATCATTTTCAGTCAGTTAACTTTCCTGGAGAATCTCTCATGACACAGG                               >  SRR4302537.6381809/1‑94 (MQ=60)
                                                                   CGTTGTCCATACCCTGTCCGCCCCCCCTCTTTAAAGTAATCACATCATTTTCAGTCAGTTAACTTTCCTGGAGAATCTCTCATGACACAGGCAG                            >  SRR4302537.2696662/1‑94 (MQ=60)
                                                                    GTTGTCCATACCCTGTCCGCCCCCCCTCTTTAAAGTAATCACATCATTTTCAGTCAGTTAACTTTCCTGGAGAATCTCTCATGACACAGGCAGA                           >  SRR4302537.4007578/1‑94 (MQ=60)
                                                                      TGTCCATACCCTGTCCGCCCCCCCTCTTTAAAGTAATCACATCATTTTCAGTCAGTTAACTTTCCTGGAGAATCTCTCATGACACAGGCAGAAC                         <  SRR4302537.5377604/94‑1 (MQ=60)
                                                                      TGTCCATACCCTGTCCGCCCCCCCTCTTTAAAGTAATCACATCATTTTCAGTCAGTTAACTTTCCTGGAGAATCTCTCATGACACAGGCAGAAC                         <  SRR4302537.1060666/94‑1 (MQ=60)
                                                                      TGTCCATACCCTGTCCGCCCCCCCTCTTTAAAGTAATCACATCATTTTCAGTCAGTTAACTTTCCTGGAGAATCTCTCATAACACAGGCAGAAC                         <  SRR4302537.1354176/94‑1 (MQ=59)
                                                                       GTCCATACCCTGTCCGCCCCCCCTCTTTAAAGTAATCACATCATTTTCAGTCAGTTAACTTTCCTGGAGAATCTCTCATGACACAGGCAGAACG                        >  SRR4302537.1309791/1‑94 (MQ=60)
                                                                       GTCCATACCCTGTCCGCCCCCCCTCTTTAAAGTAATCACATCATTTTCAGTCAGTTAACTTTCCTGGAGAATCTCTCATGACACAGGCAGAACG                        >  SRR4302537.798629/1‑94 (MQ=60)
                                                                       GTCCATACCCTGTCCGCCCCCCCTCTTTAAAGTAATCACATCATTTTCAGTCAGTTAACTTTCCTGGAGAATCTCTCATGACACAGGCAGAACG                        >  SRR4302537.1377145/1‑94 (MQ=60)
                                                                       GTCCATACCCTGTCCGCCCCCCCTCTTTAAAGTAATCACATCATTTTCAGTCAGTTAACTTTCCTGGAGAATCTCTCATGACACAGGCAGAACG                        <  SRR4302537.3281647/94‑1 (MQ=60)
                                                                       GTCCATACCCTGTCCG‑CCCCCCTCTTTAAAGTAATCACATCATTTTCAGTCAGTTAACTTTCCTGGAGAATCTCTCATGACACAGGCAGAACGC                       >  SRR4302537.2641254/1‑94 (MQ=60)
                                                                        TCCATACCCTGTCCGCCCCCCCTCTTTAAAGTAATCACATCATTTTCAGTCAGTTAACTTTCCTGGAGAATCTCTCATGACACAGGCAGAACGC                       >  SRR4302537.990518/1‑94 (MQ=60)
                                                                        TCCATACCCTGTCCGCCCCCCCTCTTTAAAGTAATCACATCATTTTCAGTCAGTTAACTTTCCTGGAGAATCTCTCATGACACAGGCAGAACGC                       >  SRR4302537.2969759/1‑94 (MQ=60)
                                                                         CCATACCCTGTCCGCCCCCCCTCTTTAAAGTAATCACATCATTTTCAGTCAGTTAACTTTCCTGGAGAATCTCTCATGACACAGGCAGAACGCC                      >  SRR4302537.1659957/1‑94 (MQ=60)
                                                                         CCATACCCTGTCCGCCCCCCCTCTTTAAAGTAATCACATCATTTTCAGTCAGTTAACTTTCCTGGAGAATCTCTCATGACACAGGCAGAACGCC                      <  SRR4302537.4918643/94‑1 (MQ=60)
                                                                          CATACCCTGTCCGCCCCCCCTCTTTAAAGTAATCACATCATTTTCAGTCAGTTAACTTTCCTGGAGAATCTCTCATGACACAGGCAGAACGCCG                     >  SRR4302537.3150048/1‑94 (MQ=60)
                                                                           ATACCCTGTCCGCCCCCCCTCTTTAAAGTAATCACATCATTTTCAGTCAGTTAACTTTCCTGGAGGATCTCTCATGACACAGGCAGAAAGCCGC                    >  SRR4302537.1982521/1‑94 (MQ=56)
                                                                           ATACCCTGTCCGCCCCCCCTCTTTAAAGTAATCACATCATTTTCAGTCAGTTAACTTTCCTGGAGAATCTCTCATGACACAGGCAGAACGCCGC                    >  SRR4302537.246391/1‑94 (MQ=60)
                                                                            TACCCTGTCCGCCCCCCCTCTTTAAAGTAATCACATCATTTTCAGTCAGTTAACTTTCCTGGAGAATCTCTCATGACACAGGCAGAACGCCGCC                   >  SRR4302537.207538/1‑94 (MQ=60)
                                                                              CCCTGTCCG‑CCCCCCTCTTTAAAGTAATCACATCATTTTCAGTCAGTTAACTTTCCTGGAGAATCTCTCATGACACAGGCAGAACGCCGCCATG                >  SRR4302537.3259567/1‑94 (MQ=60)
                                                                                 cgtccgcCCCCCCTCTTTAAAGTAATCACATCATTTTCAGTCAGTTAACTTTCCTGGAGAATCTCTCATGACACAGGCAGAACGCCGCCATGAC              <  SRR4302537.3859559/87‑1 (MQ=57)
                                                                                 cgtccgcCCCCCCTCTTTAAAGTAATCACATCATTTTCAGTCAGTTAACTTTCCTGGAGAATCTCTCATGACACAGGCAGAACGCCGCCATGAC              <  SRR4302537.4879099/87‑1 (MQ=57)
                                                                                 TGTCCGCCCCCCCTCTTTAAAGTAATCACATCATTTTCAGTCAGTTATCTTTCCTGGAGAATCTCTCATGACACAGGCAGAACGCCGCCATGAC              <  SRR4302537.3585943/94‑1 (MQ=50)
                                                                                  GTCCGCCCCCCCTCTTTAAAGTAATCACATCATTTTCAGTCAGTTAACTTTCCTGGAGAATCTCTCATGACACAGGCAGAACGCCGCCATGACC             >  SRR4302537.5803927/1‑94 (MQ=52)
                                                                                      gcCCCCCCTCTTTAAAGTAATCACATCATTTTCAGTCAGTTAACTTTCCTGGAGAATCTCTCATGACACAGGCAGAACGCCGCCATGACCGGCT         <  SRR4302537.3468650/92‑1 (MQ=56)
                                                                                      gcCCCCCCTCTTTAAAGTAATCACATCATTTTCAGTCAGTTAACTTTCCTGGAGAATCTCTCATGACACAGGCAGAACGCCGCCATGACCGGCT         <  SRR4302537.3690764/92‑1 (MQ=56)
                                                                                      C‑CCCCCCTCTTTAAAGTAATCACATCATTTTCAGTCAGTTAACTTTCCTGGAGAATCTCTCATGACACAGGCAGAACGCCGCCATGACCGGCTG        <  SRR4302537.5859442/94‑1 (MQ=55)
                                                                                      C‑CCCCCCTCTTTAAAGTAATCACATCATTTTCAGTCAGTTAACTTTCCTGGAGAATCTCTCATGACACAGGCAGAACGCCGCCATGACCGGCTG        >  SRR4302537.1629503/1‑94 (MQ=55)
                                                                                         CCCCCTCTTTAAAGTAATCACATCATTTTCAGTCAGTTAACTTTCCTGGAGAATCTCTCATGACACAGGCAGAACGCCGCCATGACCGGCTGGC      >  SRR4302537.3597908/1‑94 (MQ=52)
                                                                                         CCCCCTCTTAAAAGTAATCACATCATTTTCAGTCAGTTAACTTTCCTGGAGAATCTCTCATGACACAGGCAGAACGCCGCCATGACCGGCTGGC      >  SRR4302537.5000694/1‑94 (MQ=50)
                                                                                          CCCCTCTTTAAAGTAATCACATCATTTTCAGTCAGTTAACTTTCCTGGAGAATCTCTCATGACACAGGCAGAACGCCGCCATGCCCGGCTGGCT     >  SRR4302537.2087142/1‑94 (MQ=50)
                                                                                          CCCCTCTTTAAAGTAATCACATCATTTTCAGTCAGTTAACTTTCCTGGAGAATCTCTCATGACACAGGCAGAACGCCGCCATGACCGGCTGGCT     <  SRR4302537.3409790/94‑1 (MQ=52)
                                                                                           CCCTCTTTAAAGTAATCACATCATTTTCAGTCAGTTAACTTTCCTGGAGAATCTCTCATGACACAGGCAGAACGCCGCCATGACCGGCTGGCTG    <  SRR4302537.6153965/94‑1 (MQ=52)
                                                                                            CCTCTTTAAAGTAATCACATCATTTTCAGTCAGTTAACTTTCCTGGAGAATCTCTCATGACACAGGCAGAACGCCGCCATGACCGGCTGGCTGT   >  SRR4302537.717144/1‑94 (MQ=52)
                                                                                            CCTCTTTAAAGTAATCACATCATTTTCAGTCAGTTAACTTTCCTGGAGAATCTCTCATGACACAGGCAGAACGCCGCCATGACCGGCTGGCTGT   >  SRR4302537.874513/1‑94 (MQ=52)
                                                                                             CTCTTTAAAGTAATCACATCATTTTCAGTCAGTTAACTTTCCTGGAGAATCTCTCATGACACAGGCAGAACGCCGCCCTGACCGGCTGGCTGTC  >  SRR4302537.2400473/1‑94 (MQ=50)
                                                                                             |                                                                                             
AATCCCCCGGTTATAGCTTTTAACCCCCGTTACATCTGGCTTTTGCAGAAATAAAAAATAGTTTCTGCGTTGTCCATACCCTGTCCG‑CCCCCCTCTTTAAAGTAATCACATCATTTTCAGTCAGTTAACTTTCCTGGAGAATCTCTCATGACACAGGCAGAACGCCGCCATGACCGGCTGGCTGTC  >  NC_000913/2767577‑2767762

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 28 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑