Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A1 F1 I2 R1	882	254.4	12957588	98.0%	12698436	93.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,815,093	G→A	P145S (CCA→TCA)	yqaB ←	putative phosphatase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,815,093	0	G	A	97.7%	890.5 / 4.0	255	P145S (CCA→TCA)	yqaB	putative phosphatase
Reads supporting (aligned to +/- strand): ref base G (2/4); new base A (127/122); total (129/126)
Fisher's exact test for biased strand distribution p-value = 4.43e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.36e-01

Only 100 of 274 total aligned reads displayed.

CCGCCTGAATACCGAAATCGGCATCTTCAAAGACCACACACTGCGTCGGTTGCACGCCCATACGCTGCGCGCACAACAAAAATGTGTCTGGCGCGGGTTTATGGTGTTTGACGTGATCGGCAGCGACGACGGCGTCAAAATAATGGCGTAATCCCAGGTGCGCCAGCAATGCCTCAGCGATGG  >  NC_000913/2815003‑2815185

ccgccTGAATACCGAAATCGGCATCTTCAAAGACCACACACTGCGTCGGTTGCACGCCCATACGCTGCGCGCACAACAAAAATGTGTCTGacg                                                                                            >  1:4159484/1‑93 (MQ=255)

 cgccTGAATACCGAAATCGGCATCTTCAAAGACCACACACTGCGTCGGTTGCACGCCCATACGCTGCGCGCACAACAAAAATGTGTCTGacgc                                                                                           <  1:738038/93‑1 (MQ=255)

  gccTGAATACCGAAATCGGCATCTTCAAAGACCACACACTGCGTCGGTTGCACGCCCATACGCTGCGCGCACAACAAAAATGTGTCTGAcgcg                                                                                          >  1:3721939/1‑93 (MQ=255)

    cTGAATACCGAAATCGGCATCTTCAAAGACCACACACTGCGTCGGTTGCACGCCCATACGCTGCGCGCACAACAAAAATGTGTCTGACGCggg                                                                                        <  2:828355/93‑1 (MQ=255)

    cTGAATACCGAAATCGGCATCTTCAAAGACCACACACTGCGTCGGTTGCACGCCCATACGCTGCGCGCACAACAAAAATGTGTCTGACGCggg                                                                                        <  2:6290346/93‑1 (MQ=255)

     tGAATACCGAAATCGGCATCTTCAAAGACCACACACTGCGTCGGTTGCACGCCCATACGCTGCGCGCACAACAAAAATGTGTCTGACGCGGGt                                                                                       >  2:4889/1‑93 (MQ=255)

     tGAATACCGAAATCGGCATCTTCAAAGACCACACACTGCGTCGGTTGCACGCCCATACGCTGCGCGCACAACAAAAATGTGTCTGACGCGGGt                                                                                       <  1:168626/93‑1 (MQ=255)

     tGAATACCGAAATCGGCATCTTCAAAGACCACACACTGCGTCGGTTGCACGCCCATACGCTGCGCGCACAACAAAAATGTGTCTGACGCGGGt                                                                                       >  1:4676102/1‑93 (MQ=255)

      ggaTACCGAAATCGGCATCTTCAAAGACCACACACTGCGTCGGTTGCACGCCCATACGCTGCGCGCACAACAAAAATGTGTCTGACGCGGGtt                                                                                      >  2:4070632/3‑93 (MQ=255)

       aaTACCGAAATCGGCATCTTCAAAGACCACACACTGCGTCGGTTGCACGCCCATACGCTGCGCGCACAACAAAAATGTGTCTGACGCGGGttt                                                                                     <  1:3877675/93‑1 (MQ=255)

        aTACCGAAATCGGCATCTTCAAAGACCACACACTGCGTCGGTTGCACGCCCATACGCTGCGCGCACAACAAAAATGTGTCTGACGCGGGTTTa                                                                                    >  2:4837146/1‑93 (MQ=255)

         tACCGAAATCGGCATCTTCAAAGACCACACACTGCGTCGGTTGCACGCCCATACGCTGCGCGCACAACAAAAATGTGTCTGACGCGGGTTTAt                                                                                   <  2:1506663/93‑1 (MQ=255)

         tACCGAAATCGGCATCTTCAAAGACCACACACTGCGTCGGTTGCACGCCCATACGCTGCGCGCACAACAAAAATGTGTCTGACGCGGGTTTAt                                                                                   <  1:1356241/93‑1 (MQ=255)

           ccGAAATCGGCATCTTCAAAGACCACACACTGCGTCGGTTGCACGCCCATACGCTGCGCGCACAACAAAAATGTGTCTGACGCGGGTTTATgg                                                                                 <  2:3830803/93‑1 (MQ=255)

            cGAAATCGGCATCTTCAAAGACCACACACTGCGTCGGTTGCACGCCCATACGCTGCGCGCACAACAAAAATGTGTCTGACGCGGGGTTATGgt                                                                                >  2:1230192/1‑93 (MQ=255)

             gAAATCGGCATCTTCAAAGACCACACACTGCGTCGGTTGCACGCCCATACGCTGCGCGCACAACAAAAATGTGTCTGACGCGGGTTTATGgtg                                                                               >  1:2544161/1‑93 (MQ=255)

               aaTCGGCATCTTCAAAGACCACACACTGCGTCGGTTGCACGCCCATACGCTGCGCGCACAACAAAAATGTGTCTGACGCGGGTTTGTGGTGtt                                                                             >  2:5536635/1‑93 (MQ=255)

               aaTCGGCATCTTCAAAGACCACACACTGCGTCGGTTGCACGCCCATACGCTGCGCGCACAACAAAAATGTGTCTGACGCGGGTTTATGGTGtt                                                                             >  1:5743287/1‑93 (MQ=255)

                aTCGGCATCTTCAAAGACCACACACTGCGTCGGTTGCACGCCCATACGCTGCGCGCACAACAAAAATGTGTCTGACGCGGGTTTATGGTGttt                                                                            <  2:4648549/93‑1 (MQ=255)

                  cGGCATCTTCAAAGACCACACACTGCGTCGGTTGCACGCCCATACGCTGCGCGCACAACAAAAATGTGTCTGACGCGGGTTTATGGTGTTTGa                                                                          >  1:3113094/1‑93 (MQ=255)

                     cATCTTCAAAGACCACACACTGCGTCGGTTGCACGCCCATACGCTGCGCGCACAACAAAAATGTGTCTGACGCGGGTTTATGGTGTTTGACGt                                                                       <  1:6475018/93‑1 (MQ=255)

                     cATCTTCAAAGACCACACACTGCGTCGGTTGCACGCCCATACGCTGCGCGCACAACAAAAATGTGTCTGACGCGGGTTTATGGTGTTTGACGt                                                                       >  1:359606/1‑93 (MQ=255)

                       tCTTCAAAGACCACACACTGCGTCGGTTGCACGCCCATACGCTGCGCGCACAACAAAAATGTGTCTGACGCGGGTTTATGGTGTTTGACGTGa                                                                     >  1:2924665/1‑93 (MQ=255)

                       tCTTCAAAGACCACACACTGCGTCGGTTGCACGCCCATACGCTGCGCGCACAACAAAAATGTGTCTGACGCGGGTTTATGGTGTTTGACGTGa                                                                     <  2:5282827/93‑1 (MQ=255)

                       tCTTCAAAGACCACACACTGCGTCGGTTGCACGCCCATACGCTGCGCGCACAACAAAAATGTGTCTGACGCGGGTTTATGGTGTTTGACGTGa                                                                     >  1:5561739/1‑93 (MQ=255)

                       tCTTCAAAGACCACACACTGCGTCGGTTGCACGCCCATACGCTGCGCGCACAACAAAAATGTGTCTGACGCGGGTTTATGGTGTTTGACGTGa                                                                     >  2:2209149/1‑93 (MQ=255)

                       tCTTCAAAGACCACACACTGCGTCGGTTGCACGCCCATACGCTGCGCGCACAACAAAAATGTGTCTGACGCGGGTTTATGGTGTTTGACGTGa                                                                     >  2:2761161/1‑93 (MQ=255)

                        cTTCAAAGACCACACACTGCGTCGGTTGCACGCCCATACGCTGCGCGCACAACAAAAATGTGTCTGACGCGGGTTTATGGTGTTTGACGTGAt                                                                    <  2:4915749/93‑1 (MQ=255)

                        cTTCAAAGACCACACACTGCGTCGGTTGCACGCCCATACGCTGCGCGCACAACAAAAATGTGTCTGACGCGGGTTTATGGTGTTTGACGTGAt                                                                    <  2:2256119/93‑1 (MQ=255)

                         ttCAAAGACCACACACTGCGTCGGTTGCACGCCCATACGCTGCGCGCACAACAAAAATGTGTCTGACGCGGGTTTATGGTGTTTGACGTGATc                                                                   <  2:5790040/93‑1 (MQ=255)

                         ttCAAAGACCACACACTGCGTCGGTTGCACGCCCATACGCTGCGCGCACAACAAAAATGTGTCTGACGCGGGTTTATGGTGTTTGACGTGATc                                                                   >  1:3993019/1‑93 (MQ=255)

                          taaaaGACCACACACTGCGTCGGTTGCACGCCCATACGCTGCGCGCACAACAAAAATGTGTCTGACGCGGGTTTATGGTGTTTGACGTGATCg                                                                  >  2:5698188/3‑93 (MQ=255)

                          tCAAAGACCACACACTGCGTCGGTTGCACGCCCATACGCTGCGCGCACAACAAAAATGTGTCTGACGCGGGTTTATGGTGTTTGACGTGATCg                                                                  <  2:5412687/93‑1 (MQ=255)

                          tCAAAGACCACACACTGCGTCGGTTGCACGCCCATACGCTGCGCGCACAACAAAAATGTGTCTGACGCGGGTTTATGGTGTTTGACGTGATCg                                                                  >  2:295024/1‑93 (MQ=255)

                           cAAAGACCACACACTGCGTCGGTTGCACGCCCATACGCTGCGCGCACAACAAAAATGTGTCTGACGCGGGTTTATGGTGTTTGACGTGATCgg                                                                 <  2:4689569/93‑1 (MQ=255)

                             aaGACCACACACTGCGTCGGTTGCACGCCCATACGCTGCGCGCACAACAAAAATGTGTCTGACGCGGGTTTATGGTGTTTGACGTGATCGGCa                                                               >  1:4598390/1‑93 (MQ=255)

                             aaGACCACACACTGCGTCGGTTGCACGCCCATACGCTGCGCGCACAACAAAAATGTGTCTGACGCGGGTTTATGGTGTTTGACGTGATCGGCa                                                               <  1:2334381/93‑1 (MQ=255)

                             aaGACCACACACTGCGTCGGTTGCACGCCCATACGCTGCGCGCACAACAAAAATGTGTCTGACGCGGGTTTATGGTGTTTGACGTGATCGGCa                                                               <  2:4510715/93‑1 (MQ=255)

                             aaGACCACACACTGCGTCGGTTGCACGCCCATACGCTGCGCGCACAACAAAAATGTGTCTGACGCGGGTTTATGGTGTTTGACGTGATCGGCa                                                               <  2:5063217/93‑1 (MQ=255)

                              aGACCACACACTGCGTCGGTTGCACGCCCATACGCTGCGCGCACAACAAAAATGTGTCTGACGCGGGTTTATGGTGTTTGACGTGATCGGCAg                                                              <  2:2730858/93‑1 (MQ=255)

                              aGACCACACACTGCGTCGGTTGCACGCCCATACGCTGCGCGCACAACAAAAATGTGTCTGACGCGGGTTTATGGTGTTTGACGTGATCGGCAg                                                              >  1:2245743/1‑93 (MQ=255)

                                   acacacTGCGTCGGTTGCACGCCCATACGCTGCGCGCACAACAAAAATGTGTCTGACGCGGGTTTATGGTGTTTGACGTGATCGGCAGcgacg                                                         >  1:248902/1‑93 (MQ=255)

                                    cacacTGCGTCGGTTGCACGCCCATACGCTGCGCGCACAACAAAAATGTGTCTGACGCGGGTTTATGGTGTTTGACGTGATCGGCAGcgacga                                                        <  1:3399261/93‑1 (MQ=255)

                                     acacTGCGTCGGTTGCACGCCCATACGCTGCGCGCACAACAAAAATGTGTCTGACGCGGGTTTATGGTGTTTGACGTGATCGGCAGCgacgac                                                       <  1:396388/93‑1 (MQ=255)

                                      cacTGCGTCGGTTGCACGCCCATACGCTGCGCGCACAACAAAAATGTGTCTGACGCGGGTTTATGGTGTTTGACGTGATCGGCAGCGacgacg                                                      >  1:130869/1‑93 (MQ=255)

                                        cTGCGTCGGTTGCACGCCCATACGCTGCGCGCACAACAAAAATGTGTCTGACGCGGGTTTATGGTGTTTGACGTGATCGGCAGCGACGACGGc                                                    >  2:5806281/1‑93 (MQ=255)

                                           cGTCGGTTGCACGCCCATACGCTGCGCGCACAACAAAAATGTGTCTGACGCGGGTTTATGGTGTTTGACGTGATCGGCAGCGACGACGGCGTc                                                 <  1:5412237/93‑1 (MQ=255)

                                            gTCGGTTGCACGCCCATACGCTGCGCGCACAACAAAAATGTGTCTGACGCGGGTTTATGGTGTTTGACGTGATCGGCAGCGACGACGGCGTCa                                                >  1:5778154/1‑93 (MQ=255)

                                            gTCGGTTGCACGCCCATACGCTGCGCGCACAACAAAAATGTGTCTGACGCGGGTTTATGGTGTTTGACGTGATCGGCAGCGACGACGGCGTCa                                                >  1:1410136/1‑93 (MQ=255)

                                             tCGGTTGCACGCCCATACGCTGCGCGCACAACAAAAATGTGTCTGGCGCGGGTTTATGGTGTTTGACGTGATCGGCAGCGACGACGGCGTCaa                                               >  2:6002071/1‑93 (MQ=255)

                                               ggTTGCACGCCCATACGCTGCGCGCACAACAAAAATGTGTCTGACGCGGGTTTATGGTGTTTGACGTGATCGGCAGCGACGACGGCGTCaaaa                                             <  1:1793374/93‑1 (MQ=255)

                                                gTTGCACGCCCATACGCTGCGCGCACAACAAAAATGTGTCTGACGCGGGTTTATGGTGTTTGACGTGATCGGCAGCGACGACGGCGTCAAaat                                            >  1:5130629/1‑93 (MQ=255)

                                                  tGCACGCCCATACGCTGCGCGCACAACAAAAATGTGTCTGACGCGGGTTTATGGTGTTTGACGTGATCGGCAGCGACGACGGCGTCAAaataa                                          >  2:6189592/1‑93 (MQ=255)

                                                  tGCACGCCCATACGCTGCGCGCACAACAAAAATGTGTCTGACGCGGGTTTATGGTGTTTGACGTGATCGGCAGCGACGACGGCGTCAAaataa                                          >  1:4863503/1‑93 (MQ=255)

                                                   gCACGCCCATACGCTGCGCGCACAACAAAAATGTGTCTGACGCGGGTTTATGGTGTTTGACGTGATCGGCAGCGACGACGGCGTCAAaataat                                         <  2:3611703/93‑1 (MQ=255)

                                                    cACGCCCATACGCTGCGCGCACAACAAAAATGTGTCTGACGCGGGTTTATGGTGTTTGACGTGATCGGCAGCGACGACGGCGTCAAAATAATg                                        <  1:5311268/93‑1 (MQ=255)

                                                    cACGCCCATACGCTGCGCGCACAACAAAAATGTGTCTGACGCGGGTTTATGGTGTTTGACGTGATCGGCAGCGACGACGGCGTCAAAATAATg                                        <  1:5050956/93‑1 (MQ=255)

                                                    cACGCCCATACGCTGCGCGCACAACAAAAATGTGTCTGACGCGGGTTTATGGTGTTTGACGTGATCGGCAGCGACGACGGCGTCAAAATAATg                                        >  1:4325292/1‑93 (MQ=255)

                                                     aCGCCCATACGCTGCGCGCACAACAAAAATGTGTCTGACGCGGGTTTATGGTGTTTGACGTGATCGGCAGCGACGACGGCGTCAAAATAATgg                                       >  2:3341235/1‑93 (MQ=255)

                                                     aCGCCCATACGCTGCGCGCACAACAAAAATGTGTCTGACGCGGGTTTATGGTGTTTGACGTGATCGGCAGCGACGACGGCGTCAAAATAATgg                                       >  1:1772651/1‑93 (MQ=255)

                                                      cGCCCATACGCTGCGCGCACAACAAAAATGTGTCTGACGCGGGTTTATGGTGTTTGACGTGATCGGCAGCGACGACGGCGTCAAAATAATGGc                                      >  1:1468536/1‑93 (MQ=255)

                                                      cGCCCATACGCTGCGCGCACAACAAAAATGTGTCTGACGCGGGTTTATGGTGTTTGACGTGATCGGCAGCGACGACGGCGTCAAAATAATGGc                                      <  1:5855048/93‑1 (MQ=255)

                                                       gCCCATACGCTGCGCGCACAACAAAAATGTGTCTGACGCGGGTTTATGGTGTTTGACGTGATCGGCAGCGACGACGGCGTCAAAATAATGGCg                                     <  2:4047929/93‑1 (MQ=255)

                                                        cccATACGCTGCGCGCACAACAAAAATGTGTCTGACGCGGGTTTATGGTGTTTGACGTGATCGGCAGCGACGACGGCGTCAAAATAATGGCGt                                    >  2:4413992/1‑93 (MQ=255)

                                                         ccATACGCTGCGCGCACAACAAAAATGTGTCTGACGCGGGTTTATGGTGTTTGACGTGATCGGCAGCGACGACGGCGTCAAAATAATGGCGTa                                   <  2:1627121/93‑1 (MQ=255)

                                                         ccATACGCTGCGCGCACAACAAAAATGTGTCTGACGCGGGCTTATGGTGTTTGACGTGATCGGCAGCGACGACGGCGTCAAAATAATGGCGTa                                   >  2:4180698/1‑93 (MQ=255)

                                                           aTACGCTGCGCGCACAACAAAAATGTGTCTGACGCGGGTTTATGGTGTTTGACGTGATCGGCAGCGACGACGGCGTCAAAATAATGGCGTAAt                                 <  1:126904/93‑1 (MQ=255)

                                                            tACGCTGCGCGCACAACAAAAATGTGTCTGACGCGGGTTTATGGTGTTTGACGTGATCGGCAGCGACGACGGCGTCAAAATAATGGCGTAATc                                >  1:6259238/1‑93 (MQ=255)

                                                            tACGCTGCGCGCACAACAAAAATGTGTCTGACGCGGGTTTATGGTGTTTGACGTGATCGGCAGCGACGACGGCGTCAAAATAATGGCGTAATc                                >  2:4612258/1‑93 (MQ=255)

                                                             aCGCTGCGCGCACAACAAAAATGTGTCTGACGCGGGTTTATGGTGTTTGACGTGATCGGCAGCGACGACGGCGTCAAAATATTGGCGTAATcc                               >  2:4757978/1‑93 (MQ=255)

                                                             aCGCTGCGCGCACAACAAAAATGTGTCTGACGCGGGTTTATGGTGTTTGACGTGATCGGCAGCGACGACGGCGTCAAAATAATGGCGTAATcc                               <  1:5945052/93‑1 (MQ=255)

                                                             aCGCTGCGCGCACAACAAAAATGTGTCTGACGCGGGTTTATGGTGTTTGACGTGATCGGCAGCGACGACGGCGTCAAAATAATGGCGTAATcc                               >  1:943081/1‑93 (MQ=255)

                                                              cGCTGCGCGCACAACAAAAATGTGTCTGACGCGGGTTTATGGTGTTTGACGTGATCGGCAGCGACGACGGCGTCAAAATAATGGCGTAATccc                              <  2:255992/93‑1 (MQ=255)

                                                                cTGCGCGCACAACAAAAATGTGTCTGACGCGGGTTTATGGTGTTTGACGTGATCGGCAGCGACGACGGCGTCAAAATAATGGCGTAATCCCAg                            <  2:1137544/93‑1 (MQ=255)

                                                                 tGCGCGCACAACAAAAATGTGTCTGACGCGGGTTTATGGTGTTTGACGTGATCGGCAGCGACGACGGCGTCAAAATAATGGCGTAATCCCAgg                           <  1:3448580/93‑1 (MQ=255)

                                                                 tGCGCGCACAACAAAAATGTGTCTGACGCGGGTTTATGGTGTTTGACGTGATCGGCAGCGACGACGGCGTCAAAATAATGGCGTAATCCCAgg                           <  1:5712893/93‑1 (MQ=255)

                                                                  gcgcgcACAACAAAAATGTGTCTGACGCGGGTTTATGGTGTTTGACGTGATCGGCAGCGACGACGGCGTCAAAATAATGGCGTAATCCCAggg                          >  2:1688914/1‑92 (MQ=255)

                                                                  gcgcgcACAACAAAAATGTGTCTGACGCGGGTTTATGGTGTTTGACGTGATCGGCAGCGACGACGGCGTCAAAATAATGGCGTAATCCCAGGt                          >  1:4744760/1‑93 (MQ=255)

                                                                    gctcacaACAAAAAGGTGTCGGACGCGGGTTTATGGTGTTTGACGTGATCGGCAGCGACGACGGCGTCAAAATAATGGCGTAATCCCAGGTgc                        <  1:3174930/90‑1 (MQ=255)

                                                                    gcgcACAACAAAAATGTGTCTGACGCGGGTTTATGGTGTTTGACGTGATCGGCAGCGACGACGGCGTCAAAATAATGGCGTAATCCCAGGTgc                        <  2:1933432/93‑1 (MQ=255)

                                                                    gcgcACAACAAAAATGTGTCTGACGCGGGTTTATGGTGTTTGACGTGATCGGCAGCGACGACGGCGTCAAAATAATGGCGTAATCCCAGGTgc                        >  2:693617/1‑93 (MQ=255)

                                                                    gcgcACAACAAAAATGTGTCTGACGCGGGTTTATGGTGTTTGACGTGATCGGCAGCGACGACGGCGTCAAAATAATGGCGTAATCCCAGGTgc                        <  1:2730788/93‑1 (MQ=255)

                                                                       cacaACAAAAATGTGTCTGACGCGGGCTTATGGTGTTTGACGTGATCGGCAGCCACGACGGCGACAAAATAATGGCGTAATCCCAGGTGCGcc                     >  1:6348031/1‑93 (MQ=255)

                                                                         caacaaAAATGTGTCTGACGCGGGTTTATGGTGTTTGACGTGATCGGCAGCGACGACGGCGTCAAAATAATGGCGTAATCCCAGGTGCGCCAg                   >  1:6056641/1‑93 (MQ=255)

                                                                          aacaaAAATGTGTCTGACGCGGGTTTATGGTGTTTGACGTGATCGGCAGCGACGACGGCGTCAAAATAATGGCGTAATCCCAGGTGCGCCAGc                  >  2:1323747/1‑93 (MQ=255)

                                                                           acaaAAATGTGTCTGACGCGGGTTTATGGTGTTTGACGTGATCGGCAGCGACGACGGCGTCAAAATAATGGCGTAATCCCAGGTGCGCCAGCa                 >  1:2186054/1‑93 (MQ=255)

                                                                            caaAAATGTGTCTGACGCGGGTTTATGGTGTTTGACGTGATCGGCAGCGACGACGGCGTCAAAATAATGGCGTAATCCCAGGTGCGCCAGCaa                <  1:677245/93‑1 (MQ=255)

                                                                            caaAAATGTGTCTGACGCGGGTTTATGGTGTTTGACGTGATCGGCAGCGACGACGGCGTCAAAATAATGGCGTAATCCCAGGTGCGCCAGCaa                <  1:1184980/93‑1 (MQ=255)

                                                                            aaaaaaTGTGTCTGACGCGGGTTTATGGTGTTTGACGTGATCGGCAGCGACGACGGCGTCAAAATAATGGCGTAATCCCAGGTGCGCCAGCaa                <  2:775550/92‑1 (MQ=255)

                                                                             aaaaaTGTGTCTGACGCGGGGTTATGGTGTTTGACGTGATCGGCAGCGACGACGGCGTCAAAATAAGCGCGTAATCCCAGGTGCGCCAGCAAt               >  2:5219563/1‑93 (MQ=255)

                                                                              aaaaTGTGTCTGACGCGGGTTTATGGTGTTTGACGTGGTCGGCAGCGACGACGGCGTCAAAATAATGGCGTAATCCCAGGTGCGCCAGCAATg              >  2:3066311/1‑93 (MQ=255)

                                                                              aaaaTGTGTCTGACGCGGGTTTATGGTGTTTGACGTGATCGGCAGCGACGACGGCGTCAAAATAATGGCGTAATCCCAGGTGCGCCAGCAATg              >  1:1109917/1‑93 (MQ=255)

                                                                               aaaTGTGTCTGACGCGGGTTTATGGTGTTTGACGTGATCGGCAGCGACGACGGCGTCAAAATAATGGCGTAATCCCAGGTGCGCCAGCAATGc             <  1:2112778/93‑1 (MQ=255)

                                                                               aaaTGTGTCTGACGCGGGTTTATGGTGTTTGACGTGATCGGCAGCGACGACGGCGTCAAAATAATGGCGTAATCCCAGGTGCGCCAGCAATGc             <  1:6047733/93‑1 (MQ=255)

                                                                                aaTGTGTCTGACGCGGGTTTATGGTGTTTGACGTGATCGGCAGCGACGACGGCGTCAAAATAATGGCGTAATCCCAGGTGCGCCAGCAATGcc            >  2:3188201/1‑93 (MQ=255)

                                                                                 aTGTGTCTGACGCGGGTTTATGGTGTTTGACGTGATCGGCAGCGACGACGGCGTCAAAATAATGGCGTAATCCCAGGTGCGCCAGCAATGCCt           <  2:2372841/93‑1 (MQ=255)

                                                                                  tgtgTCTGACGCGGGTTTATGGTGTTTGACGTGATCGGCAGCGACGACGGCGTCAAAATAATGGCGTAATCCCAGGTGCGCCAGCAATGCCTc          >  1:2664901/1‑93 (MQ=255)

                                                                                   gtgtCTGACGCGGGTTTATGGTGTTTGACGTGATCGGAAGCGACGACGGCGTCAAAATAATGGCGTAATCCCAGGTGCGCCAGCAATGCCTCa         <  1:4126575/93‑1 (MQ=255)

                                                                                    tgtCTGACGCGGGTTTATGGTGTTTGACGTGATCGGCAGCGACGACGGCGTCAAAATAATGGCGTAATCCCAGGTGCGCCAGCAATGCCTCAg        >  1:4517527/1‑93 (MQ=255)

                                                                                      tCTGACGCGGGTTTATGGTGTTTGACGTGATCGGCAGCGACGAAGGCGTCAAAATAATGGCGTAATCCCAGGTGCGCCAGCAATGCCTCAGCg      <  2:4240453/93‑1 (MQ=255)

CCGCCTGAATACCGAAATCGGCATCTTCAAAGACCACACACTGCGTCGGTTGCACGCCCATACGCTGCGCGCACAACAAAAATGTGTCTGGCGCGGGTTTATGGTGTTTGACGTGATCGGCAGCGACGACGGCGTCAAAATAATGGCGTAATCCCAGGTGCGCCAGCAATGCCTCAGCGATGG  >  NC_000913/2815003‑2815185

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 22 ≤` `ATCG/ATCG` `< 29 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

BRESEQ :: bam2aln output

GGGCCGCCTGAATACCGAAATCGGCATCTTCAAAGACCACACACTGCGTCGGTTGCACGCCCATACGCTGCGCGCACAACAAAAATGTGTCTGGCGCGGGTTTATGGTGTTTGACGTGATCGGCAGCGACGACGGCGTCAAAATAATGGCGTAATCCCAGGTGCGCCAGCAATGCCTCAGCGATGGC  >  NC_000913/2815000‑2815186

GGGCCGCCTGAATACCGAAATCGGCATCTTCAAAGACCACACACTGCGTCGGTTGCACGCCCATACGCTGCGCGCACAACAAAAATGTGTCTGA                                                                                               <  SRR4302537.3128889/94‑1 (MQ=60)

GGGCCGCCTGAATACCGAAATCGGCATCTTCAAAGACCACACACTGCGTCGGTTGCACGCCCATACGCTGCGCGCACAACAAAAATGTGTCTGA                                                                                               >  SRR4302537.1512982/1‑94 (MQ=60)

  GCCGCCTGAATACCGAAATCGGCATCTTCAAAGACCACACACTGCGTCGGTTGCACGCCCATACGCTGCGCGCACAACAAAAATGTGTCTGACG                                                                                             <  SRR4302537.2920621/94‑1 (MQ=60)

  GCCGCCTGAATACCGAAATCGGCATCTTCAAAGACCACACACTGCGTCGGTTGCACGCCCATACGCTGCGCGCACAACAAAAATGTGTCTGACG                                                                                             <  SRR4302537.6212114/94‑1 (MQ=60)

  GCCGCCTGAATACCGAAATCGGCATCTTCAAAGACCACACACTGCGTCGGTTGCACGCCCATACGCTGCGCGCACAACAAAAATGTGTCTGACG                                                                                             <  SRR4302537.1658705/94‑1 (MQ=60)

  GCCGCCTGAATACCGAAATCGGCATCTTCAAAGACCACACACTGCGTCGGTTGCACGCCCATACGCTGCGCGCACAACAAAAATGTGTCTGACG                                                                                             <  SRR4302537.157879/94‑1 (MQ=60)

  GCCGCCTGAATACCGAAATCGGCATCTTCAAAGACCACACACTGCGTCGGTTGCACGCCCATACGCTGCGCGCACAACAAAAATGTGTCTGACG                                                                                             >  SRR4302537.4188533/1‑94 (MQ=60)

    CGCCTGAATACCGAAATCGGCATCTTCAAAGACCACACACTGCGTCGGTTGCACGCCCATACGCTGCGCGCACAACAAAAATGTGTCTGACGCG                                                                                           <  SRR4302537.743068/94‑1 (MQ=60)

    CGCCTGAATACCGAAATCGGCATCTTCAAAGACCACACACTGCGTCGGTTGCACGCCCATACGCTGCGCGCACAACAAAAATGTGTCTGACGCG                                                                                           <  SRR4302537.3046205/94‑1 (MQ=60)

    CGCCTGAATACCGAAATCGGCATCTTCAAAGACCACACACTGCGTCGGTTGCACGCCCATACGCTGCGCGCACAACAAAAATGTGTCTGACGCG                                                                                           <  SRR4302537.6076691/94‑1 (MQ=60)

    CGCCTGAATACCGAAATCGGCATCTTCAAAGACCACACACTGCGTCGGTTGCACGCCCATACGCTGCGCGCACAACAAAAATGTGTCTGACGCG                                                                                           >  SRR4302537.3748089/1‑94 (MQ=60)

    CGCCTGAATACCGAAATCGGCATCTTCAAAGACCACACACTGCGTCGGTTGCACGCCCATACGCTGCGCGCACAACAAAAATGTGTCTGACGCG                                                                                           >  SRR4302537.1739801/1‑94 (MQ=60)

    CGCCTGAATACCGAAATCGGCATCTTCAAAGACCACACACTGCGTCGGTTGCACGCCCATACGCTGCGCGCACAACAAAAATGTGTCTGACGCG                                                                                           <  SRR4302537.5459760/94‑1 (MQ=60)

    CGCCTGAATACCGAAATCGGCATCTTCAAAGACCACACACTGCGTCGGTTGCACGCCCATACGCTGCGCGCACAACAAAAATGTGTCTGACGCG                                                                                           <  SRR4302537.142606/94‑1 (MQ=60)

     GCCTGAATACCGAAATCGGCATCTTCAAAGACCACACACTGCGTCGGTTGCACGCCCATACGCTGCGCGCACAACAAAAATGTGTCTGACGCGG                                                                                          >  SRR4302537.13816/1‑94 (MQ=60)

     GCCTGAATACCGAAATCGGCATCTTCAAAGACCACACACTGCGTCGGTTGCACGCCCATACGCTGCGCGCACAACAAAAATGTGTCTGACGCGG                                                                                          >  SRR4302537.3190050/1‑94 (MQ=60)

     GCCTGAATACCGAAATCGGCATCTTCAAAGACCACACACTGCGTCGGTTGCACGCCCATACGCTGCGCGCACAACAAAAATGTGTCTGACGCGG                                                                                          >  SRR4302537.5258626/1‑94 (MQ=60)

     GCCTGAATACCGAAATCGGCATCTTCAAAGACCACACACTGCGTCGGTTGCACGCCCATACGCTGCGCGCACAACAAAAATGTGTCTGACGCGG                                                                                          >  SRR4302537.5876674/1‑94 (MQ=60)

       CTGAATACCGAAATCGGCATCTTCAAAGACCACACACTGCGTCGGTTGCACGCCCATACGCTGCGCGCACAACAAAAATGTGTCTGACGCGGGT                                                                                        >  SRR4302537.4708451/1‑94 (MQ=60)

       CTGAATACCGAAATCGGCATCTTCAAAGACCACACACTGCGTCGGTTGCACGCCCATACGCTGCGCGCACAACAAAAATGTGTCTGACGCGGGT                                                                                        >  SRR4302537.2857005/1‑94 (MQ=60)

       CTGAATACCGAAATCGGCATCTTCAAAGACCACACACTGCGTCGGTTGCACGCCCATACGCTGCGCGCACAACAAAAATGTGTCTGACGCGGGT                                                                                        <  SRR4302537.2544575/94‑1 (MQ=60)

        TGAATACCGAAATCGGCATCTTCAAAGACCACACACTGCGTCGGTTGCACGCCCATACGCTGCGCGCACAACAAAAATGTGTCTGACGCGGGTT                                                                                       >  SRR4302537.4713999/1‑94 (MQ=60)

        TGAATACCGAAATCGGCATCTTCAAAGACCACACACTGCGTCGGTTGCACGCCCATACGCTGCGCGCACAACAAAAATGTGTCTGACGCGGGTT                                                                                       >  SRR4302537.854689/1‑94 (MQ=60)

        TGAATACCGAAATCGGCATCTTCAAAGACCACACACTGCGTCGGTTGCACGCCCATACGCTGCGCGCACAACAAAAATGTGTCTGACGCGGGTT                                                                                       <  SRR4302537.169829/94‑1 (MQ=60)

          AATACCGAAATCGGCATCTTCAAAGACCACACACTGCGTCGGTTGCACGCCCATACGCTGCGCGCACAACAAAAATGTGTCTGACGCGGGTTTA                                                                                     <  SRR4302537.3904796/94‑1 (MQ=60)

          AATACCGAAATCGGCATCTTCAAAGACCACACACTGCGTCGGTTGCACGCCCATACGCTGCGCGCACAACAAAAATGTGTCTGACGCGGGTTTA                                                                                     <  SRR4302537.4006106/94‑1 (MQ=60)

           ATACCGAAATCGGCATCTTCAAAGACCACACACTGCGTCGGTTGCACGCCCATACGCTGCGCGCACAACAAAAATGTGTCTGACGCGGGTTTAT                                                                                    >  SRR4302537.5742706/1‑94 (MQ=60)

           ATACCGAAATCGGCATCTTCAAAGACCACACACTGCGTCGGTTGCACGCCCATACGCTGCGCGCACAACAAAAATGTGTCTGACGCGGGTTTAT                                                                                    <  SRR4302537.1301482/94‑1 (MQ=60)

           ATACCGAAATCGGCATCTTCAAAGACCACACACTGCGTCGGTTGCACGCCCATACGCTGCGCGCACAACAAAAATGTGTCTGACGCGGGTTTAT                                                                                    <  SRR4302537.1416620/94‑1 (MQ=60)

            TACCGAAATCGGCATCTTCAAAGACCACACACTGCGTCGGTTGCACGCCCATACGCTGCGCGCACAACAAAAATGTGTCTGACGCGGGTTTATG                                                                                   <  SRR4302537.1365691/94‑1 (MQ=60)

             ACCGAAATCGGCATCTTCAAAGACCACACACTGCGTCGGTTGCACGCCCATACGCTGCGCGCACAACAAAAATGTGTCTGACGCGGGTTTATGG                                                                                  <  SRR4302537.5104077/94‑1 (MQ=60)

             ACCGAAATCGGCATCTTCAAAGACCACACACTGCGTCGGTTGCACGCCCATACGCTGCGCGCACAACAAAAATGTGTCTGACGCGGGTTTATGG                                                                                  <  SRR4302537.3926527/94‑1 (MQ=60)

               CGAAATCGGCATCTTCAAAGACCACACACTGCGTCGGTTGCACGCCCATACGCTGCGCGCACAACAAAAATGTGTCTGACGCGGGTTTATGGTG                                                                                >  SRR4302537.2561993/1‑94 (MQ=60)

                 AAATCGGCATCTTCAAAGACCACACACTGCGTCGGTTGCACGCCCATACGCTGCGCGCACAACAAAAATGTGTCTGACGCGGGTTTATGGTGTT                                                                              >  SRR4302537.5782610/1‑94 (MQ=60)

                 AAATCGGCATCTTCAAAGACCACACACTGCGTCGGTTGCACGCCCATACGCTGCGCGCACAACAAAAATGTGTCTGACGCGGGTTTATGGTGTT                                                                              <  SRR4302537.633045/94‑1 (MQ=60)

                    TCGGCATCTTCAAAGACCACACACTGCGTCGGTTGCACGCCCATACGCTGCGCGCACAACAAAAATGTGTCTGACGCGGGTTTATGGTGTTTGA                                                                           >  SRR4302537.3134834/1‑94 (MQ=60)

                      GGCATCTTCAAAGACCACACACTGCGTCGGTTGCACGCCCATACGCTGCGCGCACAACAAAAATGTGTCTGACGCGGGTTTATGGTGTTTGACG                                                                         >  SRR4302537.3097107/1‑94 (MQ=60)

                      GGCATCTTCAAAGACCACACACTGCGTCGGTTGCACGCCCATACGCTGCGCGCACAACAAAAATGTGTCTGACGCGGGTTTATGGTGTTTGACG                                                                         <  SRR4302537.1792018/94‑1 (MQ=60)

                       GCATCTTCAAAGACCACACACTGCGTCGGTTGCACGCCCATACGCTGCGCGCACAACAAAAATGTGTCTGACGCGGGTTTATGGTGTTTGACGT                                                                        >  SRR4302537.362104/1‑94 (MQ=60)

                       GCATCTTCAAAGACCACACACTGCGTCGGTTGCACGCCCATACGCTGCGCGCACAACAAAAATGTGTCTGACGCGGGTTTATGGTGTTTGACGT                                                                        >  SRR4302537.5948768/1‑94 (MQ=60)

                       GCATCTTCAAAGACCACACACTGCGTCGGTTGCACGCCCATACGCTGCGCGCACAACAAAAATGTGTCTGACGCGGGTTTATGGTGTTTGACGT                                                                        >  SRR4302537.6079047/1‑94 (MQ=60)

                        CATCTTCAAAGACCACACACTGCGTCGGTTGCACGCCCATACGCTGCGCGCACAACAAAAATGTGTCTGACGCGGGTTTATGGTGTTTGACGTG                                                                       <  SRR4302537.6370595/94‑1 (MQ=60)

                        CATCTTCAAAGACCACACACTGCGTCGGTTGCACGCCCATACGCTGCGCGCACAACAAAAATGTGTCTGACGCGGGTTTATGGTGTTTGACGTG                                                                       >  SRR4302537.1364850/1‑94 (MQ=60)

                        CATCTTCAAAGACCACACACTGCGTCGGTTGCACGCCCATACGCTGCGCGCACAACAAAAATGTGTCTGACGCGGGTTTATGGTGTTTGACGTG                                                                       <  SRR4302537.6518975/94‑1 (MQ=60)

                         ATCTTCAAAGACCACACACTGCGTCGGTTGCACGCCCATACGCTGCGCGCACAACAAAAATGTGTCTGACGCGGGTTTATGGTGTTTGACGTGA                                                                      >  SRR4302537.4364198/1‑94 (MQ=60)

                         ATCTTCAAAGACCACACACTGCGTCGGTTGCACGCCCATACGCTGCGCGCACAACAAAAATGTGTCTGACGCGGGTTTATGGTGTTTGACGTGA                                                                      >  SRR4302537.5599745/1‑94 (MQ=60)

                         ATCTTCAAAGACCACACACTGCGTCGGTTGCACGCCCATACGCTGCGCGCACAACAAAAATGTGTCTGACGCGGGTTTATGGTGTTTGACGTGA                                                                      >  SRR4302537.2945208/1‑94 (MQ=60)

                           CTTCAAAGACCACACACTGCGTCGGTTGCACGCCCATACGCTGCGCGCACAACAAAAATGTGTCTGACGCGGGTTTATGGTGTTTGACGTGATC                                                                    >  SRR4302537.4020974/1‑94 (MQ=60)

                            TTCAAAGACCACACACTGCGTCGGTTGCACGCCCATACGCTGCGCGCACAACAAAAATGTGTCTGACGCGGGTTTATGGTGTTTGACGTGATCG                                                                   >  SRR4302537.3509362/1‑94 (MQ=60)

                               AAAGACCACACACTGCGTCGGTTGCACGCCCATACGCTGCGCGCACAACAAAAATGTGTCTGACGCGGGTTTATGGTGTTTGACGTGATCGGCA                                                                >  SRR4302537.3463589/1‑94 (MQ=60)

                               AAAGACCACACACTGCGTCGGTTGCACGCCCATACGCTGCGCGCACAACAAAAATGTGTCTGACGCGGGTTTATGGTGTTTGACGTGATCGGCA                                                                >  SRR4302537.4630213/1‑94 (MQ=60)

                               AAAGACCACACACTGCGTCGGTTGCACGCCCATACGCTGCGCGCACAACAAAAATGTGTCTGACGCGGGTTTATGGTGTTTGACGTGATCGGCA                                                                <  SRR4302537.4142532/94‑1 (MQ=60)

                                AAGACCACACACTGCGTCGGTTGCACGCCCATACGCTGCGCGCACAACAAAAATGTGTCTGACGCGGGTTTATGGTGTTTGACGTGATCGGCAG                                                               <  SRR4302537.5008536/94‑1 (MQ=60)

                                AAGACCACACACTGCGTCGGTTGCACGCCCATACGCTGCGCGCACAACAAAAATGTGTCTGACGCGGGTTTATGGTGTTTGACGTGATCGGCAG                                                               <  SRR4302537.2350834/94‑1 (MQ=60)

                                AAGACCACACACTGCGTCGGTTGCACGCCCATACGCTGCGCGCACAACAAAAATGTGTCTGACGCGGGTTTATGGTGTTTGACGTGATCGGCAG                                                               >  SRR4302537.2261587/1‑94 (MQ=60)

                                  GACCACACACTGCGTCGGTTGCACGCCCATACGCTGCGCGCACAACAAAAATGTGTCTGACGCGGGTTTATGGTGTTTGACGTGATCGGCAGCG                                                             <  SRR4302537.1967136/94‑1 (MQ=60)

                                   ACCACACACTGCGTCGGTTGCACGCCCATACGCTGCGCGCACAACAAAAATGTGTCTGACGCGGGTTTATGGTGTTTGACGTGATCGGCAGCGA                                                            <  SRR4302537.1124624/94‑1 (MQ=60)

                                   ACCACACACTGCGTCGGTTGCACGCCCATACGCTGCGCGCACAACAAAAATGTGTCTGACGCGGGTTTATGGTGTTTGACGTGATCGGCAGCGA                                                            >  SRR4302537.144785/1‑94 (MQ=60)

                                    CCACACACTGCGTCGGTTGCACGCCCATACGCTGCGCGCACAACAAAAATGTGTCTGGCGCGGGTTTATGGTGTTTGACGTGATCGGCAGCGAC                                                           <  SRR4302537.1297295/94‑1 (MQ=60)

                                     CACACACTGCGTCGGTTGCACGCCCATACGCTGCGCGCACAACAAAAATGTGTCTGACGCGGGTTTATGGTGTTTGACGTGATCGGCAGCGACG                                                          >  SRR4302537.2507270/1‑94 (MQ=60)

                                     CACACACTGCGTCGGTTGCACGCCCATACGCTGCGCGCACAACAAAAATGTGTCTGACGCGGGTTTATGGTGTTTGACGTGATCGGCAGCGACG                                                          >  SRR4302537.250674/1‑94 (MQ=60)

                                       CACACTGCGTCGGTTGCACGCCCATACGCTGCGCGCACAACAAAAATGTGTCTGACGCGGGTTTATGGTGTTTGACGTGATCGGCAGCGACGAC                                                        <  SRR4302537.3423338/94‑1 (MQ=60)

                                       CACACTGCGTCGGTTGCACGCCCATACGCTGCGCGCACAACAAAAATGTGTCTGACGCGGGGTTATGGTGTTTGACGTGATCGGCAGCGACGAC                                                        >  SRR4302537.783891/1‑94 (MQ=60)

                                        ACACTGCGTCGGTTGCACGCCCATACGCTGCGCGCACAACAAAAATGTGTCTGACGCGGGTTTATGGTGTTTGACGTGATCGGCAGCGACGACG                                                       <  SRR4302537.399136/94‑1 (MQ=60)

                                        ACACTGCGTCGGTTGCACGCCCATACGCTGCGCGCACAACAAAAATGTGTCTGACGCGGGTTTATGGTGTTTGACGTGATCGGCAGCGACGACG                                                       >  SRR4302537.131795/1‑94 (MQ=60)

                                        ACACTGCGTCGGTTGCACGCCCATACGCTGCGCGCACAACAAAAATGTGTCTGACGCGGGTTTATGGTGTTTGACGTGATCGGCAGCGACGACG                                                       <  SRR4302537.330436/94‑1 (MQ=60)

                                        ACACTGCGTCGGTTGCACGCCCATACGCTGCGCGCACAACAAAAATGTGTCTGACGCGGGTTTATGGTGTTTGACGTGATCGGCAGCGACGACG                                                       >  SRR4302537.1526909/1‑94 (MQ=60)

                                         CACTGCGTCGGTTGCACGCCCATACGCTGCGCGCACAACAAAAATGTGTCTGACGCGGGTTTATGGTGTTTGACGTGATCGGCAGCGACGACGG                                                      <  SRR4302537.2495655/94‑1 (MQ=60)

                                          ACTGCGTCGGTTGCACGCCCATACGCTGCGCGCACAACAAAAATGTGTCTGACGCGGGTTTATGGTGTTTGACGTGATCGGCAGCGACGACGGC                                                     >  SRR4302537.738266/1‑94 (MQ=60)

                                          ACTGCGTCGGTTGCACGCCCATACGCTGCGCGCACAACAAAAATGTGTCTGACGCGGGTTTATGGTGTTTGACGTGATCGGCAGCGACGACGGC                                                     >  SRR4302537.5424243/1‑94 (MQ=60)

                                          ACTGCGTCGGTTGCACGCCCATACGCTGCGCGCACAACAAAAATGTGTCTGACGCGGGTTTATGGTGTTTGACGTGATCGGCAGCGACGACGGC                                                     <  SRR4302537.3406118/94‑1 (MQ=60)

                                          ACTGCGTCGGTTGCACGCCCATACGCTGCGCGCACAACAAAAATGTGTCTGACGCGGGTTTATGGTGTTTGACGTGATCGGCAGCGACGACGGC                                                     <  SRR4302537.4622231/94‑1 (MQ=60)

                                           CTGCGTCGGTTGCACGCCCATACGCTGCGCGCACAACAAAAATGTGTCTGACGCGGGTTTATGGTGTTTGACGTGATCGGCAGCGACGACGGCG                                                    <  SRR4302537.1613568/94‑1 (MQ=60)

                                           CTGCGTCGGTTGCACGCCCATACGCTGCGCGCACAACAAAAATGTGTCTGACGCGGGTTTATGGTGTTTGACGTGATCGGCAGCGACGACGGCG                                                    <  SRR4302537.3963179/94‑1 (MQ=60)

                                              CGTCGGTTGCACGCCCATACGCTGCGCGCACAACAAAAATGTGTCTGACGCGGGTTTATGGTGTTTGACGTGATCGGCAGCGACGACGGCGTCA                                                 >  SRR4302537.4631732/1‑94 (MQ=60)

                                              CGTCGGTTGCACGCCCATACGCTGCGCGCACAACAAAAATGTGTCTGACGCGGGTTTATGGTGTTTGACGTGATCGGCAGCGACGACGGCGTCA                                                 >  SRR4302537.2685414/1‑94 (MQ=60)

                                              CGTCGGTTGCACGCCCATACGCTGCGCGCACAACAAAAATGTGTCTGACGCGGGTTTATGGTGTTTGACGTGATCGGCAGCGACGACGGCGTCA                                                 >  SRR4302537.1419960/1‑94 (MQ=60)

                                              CGTCGGTTGCACGCCCATACGCTGCGCGCACAACAAAAATGTGTCTGACGCGGGTTTATGGTGTTTGACGTGATCGGCAGCGACGACGGCGTCA                                                 <  SRR4302537.4232742/94‑1 (MQ=60)

                                              CGTCGGTTGCACGCCCATACGCTGCGCGCACAACAAAAATGTGTCTGACGCGGGTTTATGGTGTTTGACGTGATCGGCAGCGACGACGGCGTCA                                                 <  SRR4302537.5449140/94‑1 (MQ=60)

                                              CGTCGGTTGCACGCCCATACGCTGCGCGCACAACAAAAATGTGTCTGACGCGGGTTTATGGTGTTTGACGTGATCGGCAGCGACGACGGCGTCA                                                 >  SRR4302537.5817760/1‑94 (MQ=60)

                                                TCGGTTGCACGCCCATACGCTGCGCGCACAACAAAAATGTGTCTGACGCGGGTTTATGGTGTTTGACGTGATCGGCAGCGACGACGGCGTCAAA                                               >  SRR4302537.5763179/1‑94 (MQ=60)

                                                  GGTTGCACGCCCATACGCTGCGCGCACAACAAAAATGTGTCTGACGCGGGTTTATGGTGTTTGACGTGATCGGCAGCGACGACGGCGTCAAAAT                                             >  SRR4302537.1242411/1‑94 (MQ=60)

                                                  GGTTGCACGCCCATACGCTGCGCGCACAACAAAAATGTGTCTGACGCGGGTTTATGGTGTTTGACGTGATCGGCAGCGACGACGGCGTCAAAAT                                             >  SRR4302537.5165986/1‑94 (MQ=60)

                                                  GGTTGCACGCCCATACGCTGCGCGCACAACAAAAATGTGTCTGACGCGGGTTTATGGTGTTTGACGTGATCGGCAGCGACGACGGCGTCAAAAT                                             <  SRR4302537.1805888/94‑1 (MQ=60)

                                                  GGTTGCACGCCCATACGCTGCGCGCACAACAAAAATGTGTCTGACGCGGGTTTATGGTGTTTGACGTGATCGGCAGCGACGACGGCGTCAAAAT                                             <  SRR4302537.4348955/94‑1 (MQ=60)

                                                   GTTGCACGCCCATACGCTGCGCGCACAACAAAAATGTGTCTGACGCGGGTTTATGGTGTTTGACGTGATCGGCAGCGACGACGGCGTCAAAATA                                            >  SRR4302537.2667795/1‑94 (MQ=60)

                                                    TTGCACGCCCATACGCTGCGCGCACAACAAAAATGTGTCTGACGCGGGTTTATGGTGTTTGACGTGATCGGCAGCGACGACGGCGTCAAAATAA                                           >  SRR4302537.4897057/1‑94 (MQ=60)

                                                    TTGCACGCCCATACGCTGCGCGCACAACAAAAATGTGTCTGACGCGGGTTTATGGTGTTTGACGTGATCGGCAGCGACGACGGCGTCAAAATAA                                           <  SRR4302537.6468830/94‑1 (MQ=60)

                                                     TGCACGCCCATACGCTGCGCGCACAACAAAAATGTGTCTGACGCGGGTTTATGGTGTTTGACGTGATCGGCAGCGACGACGGCGTCAAAATAAT                                          >  SRR4302537.4715351/1‑94 (MQ=60)

                                                      GCACGCCCATACGCTGCGCGCACAACAAAAATGTGTCTGACGCGGGTTTATGGTGTTTGACGTGATCGGCAGCGACGACGGCGTCAAAATAATG                                         <  SRR4302537.5735179/94‑1 (MQ=60)

                                                      GCACGCCCATACGCTGCGCGCACAACAAAAATGTGTCTGACGCGGGTTTATGGTGTTTGACGTGATCGGCAGCGACGACGGCGTCAAAATAATG                                         >  SRR4302537.4355363/1‑94 (MQ=60)

                                                       CACGCCCATACGCTGCGCGCACAACAAAAATGTGTCTGGCGCGGGTTTATGGTGTTTGACGTGATCGGCAGCGACGACGGCGTCAAAATAATGG                                        <  SRR4302537.5113933/94‑1 (MQ=60)

                                                       CACGCCCATACGCTGCGCGCACAACAAAAATGTGTCTGACGCGGGTTTATGGTGTTTGACGTGATCGGCAGCGACGACGGCGTCAAAATAATGG                                        <  SRR4302537.5085732/94‑1 (MQ=60)

                                                       CACGCCCATACGCTGCGCGCACAACAAAAATGTGTCTGACGCGGGTTTATGGTGTTTGACGTGATCGGCAGCGACGACGGCGTCAAAATAATGG                                        <  SRR4302537.5347752/94‑1 (MQ=60)

                                                       CACGCCCATACGCTGCGCGCACAACAAAAATGTGTCTGACGCGGGTTTATGGTGTTTGACGTGATCGGCAGCGACGACGGCGTCAAAATAATGG                                        >  SRR4302537.1784998/1‑94 (MQ=60)

                                                        ACGCCCATACGCTGCGCGCACAACAAAAATGTGTCTGACGCGGGTTTATGGTGTTTGACGTGATCGGCAGCGACGACGGCGTCAAAATAATGGC                                       >  SRR4302537.1478748/1‑94 (MQ=60)

                                                         CGCCCATACGCTGCGCGCACAACAAAAATGTGTCTGACGCGGGTTTATGGTGTTTGACGTGATCGGCAGCGACGACGGCGTCAAAATAATGGCG                                      <  SRR4302537.5895208/94‑1 (MQ=60)

                                                          GCCCATACGCTGCGCGCACAACAAAAATGTGTCTGACGCGGGTTTATGGTGTTTGACGTGATCGGCAGCGACGACGGCGTCAAAATAATGGCGT                                     >  SRR4302537.1225826/1‑94 (MQ=60)

                                                           CCCATACGCTGCGCGCACAACAAAAATGTGTCTGACGCGGGTTTATGGTGTTTGACGTGATCGGCAGCGACGACGGCGTCAAAATAATGGCGTA                                    <  SRR4302537.4648263/94‑1 (MQ=60)

                                                           CCCATACGCTGCGCGCACAACAAAAATGTGTCTGACGCGGGTTTATGGTGTTTGACGTGATCGGCAGCGACGACGGCGTCAAAATAATGGCGTA                                    <  SRR4302537.3450360/94‑1 (MQ=60)

                                                            CCATACGCTGCGCGCACAACAAAAATGTGTCTGACGCGGGTTTATGGTGTTTGACGTGATCGGCAGCGACGACGGCGTCAAAATAATGGCGTAA                                   >  SRR4302537.27519/1‑94 (MQ=60)

                                                              ATACGCTGCGCGCACAACAAAAATGTGTCTGACGCGGGTTTATGGTGTTTGACGTGATCGGCAGCGACGACGGGGTCAAAATAATGGCGTAATC                                 >  SRR4302537.6463263/1‑94 (MQ=60)

                                                              ATACGCTGCGCGCACAACAAAAATGTGTCTGACGCGGGTTTATGGTGTTTGACGTGATCGGCAGCGACGACGGCGTCAAAATAATGGCGTAATC                                 <  SRR4302537.127812/94‑1 (MQ=60)

                                                              ATACGCTGCGCGCACAACAAAAATGTGTCTGACGCGGGTTTATGGTGTTTGACGTGATCGGCAGCGACGACGGCGTCAAAATAATGGCGTAATC                                 >  SRR4302537.6302218/1‑94 (MQ=60)

                                                              ATACGCTGCGCGCACAACAAAAATGTGTCTGACGCGGGCTTATGGTGTTTGACGTGATCCGCAGCGACGACCGCGTCAAAATAATGGCGTAATC                                 >  SRR4302537.6027981/1‑94 (MQ=60)

                                                               TACGCTGCGCGCACAACAAAAATGTGTCTGACGCGGGTTTATGGTGTTTGACGTGATCGGCAGCGACGACGGCGTCAAAATAATGGCGTAATCC                                >  SRR4302537.949593/1‑94 (MQ=60)

                                                                ACGCTGCGCGCACAACAAAAATGTGTCTGACGCGGGTTTATGGTGTTTGACGTGATCGGCAGCGACGACGGCGTCAAAATAATGGCGTAATCCC                               <  SRR4302537.5985858/94‑1 (MQ=60)

                                                                    TGCGCGCACAACAAAAATGTGTCTGACGCGGGTTTATGGTGTTTGACGTGATCGGCAGCGACGACGGCGTCAAAATAATGGCGTAATCCCAGGT                           <  SRR4302537.4874946/94‑1 (MQ=60)

                                                                    TGCGCGCACAACAAAAATGTGTCTGACGCGGGTTTATGGTGTTTGACGTGATCGGCAGCGACGACGGCGTCAAAATAATGGCGTAATCCCAGGT                           >  SRR4302537.4777547/1‑94 (MQ=60)

                                                                    TGCGCGCACAACAAAAATGTGTCTGACGCGGGTTTATGGTGTTTGACGTGATCGGCAGCGACGACGGCGTCAAAATAATGGCGTAATCCCAGGT                           <  SRR4302537.6163537/94‑1 (MQ=60)

                                                                    TGCGCGCACAACAAAAATGTGTCTGACGCGGGTTTATGGTGTTTGACGTGATCGGCAGCGACGACGGCGTCAAAATAATGGCGTAATCCCAGGT                           <  SRR4302537.3472974/94‑1 (MQ=60)

                                                                    TGCGCGCACAACAAAAATGTGTCTGACGCGGGTTTATGGTGTTTGACGTGATCGGCAGCGACGACGGCGTCAAAATAATGGCGTAATCCCAGGT                           >  SRR4302537.554802/1‑94 (MQ=60)

                                                                    TGCGCGCACAACAAAAATGTGTCTGACGCGGGTTTATGGTGTTTGACGTGATCGGCAGCGACGACGGCGTCAAAATAATGGCGTAATCCCAGGT                           <  SRR4302537.5751993/94‑1 (MQ=60)

                                                                     GCGCGCACAACAAAAATGTGTCTGACGCGGGTTTATGGTGTTTGACGTGATCGGCAGCGACGACGGCGTCAAAATAATGGCGTAATCCCAGGTG                          <  SRR4302537.6692/94‑1 (MQ=60)

                                                                      CGCGCACAACAAAAATGTGTCTGACGCGGGTTTATGGTGTTTGACGTGATCGGCAGCGACGACGGCGTCAAAATAATGGCGTAATCCCAGGTGC                         >  SRR4302537.2215831/1‑94 (MQ=60)

                                                                       GCTCACAACAAAAAGGTGTCGGACGCGGGTTTATGGTGTTTGACGTGATCGGCAGCGACGACGGCGTCAAAATAATGGCGTAATCCCAGGTGCG                        <  SRR4302537.3197245/94‑1 (MQ=60)

                                                                       GCGCACAACAAAAATGTGTCTGGCGCGGGTTTATGGTGTTTGACGTGATCGGCAGCGACGACGGCGTCAAAATAATGGCGTAATCCCAGGTGCG                        >  SRR4302537.2173405/1‑94 (MQ=60)

                                                                       GCGCACAACAAAAATGTGTCTGACGCGGGTTTATGGTGTTTGACGTGATCGGCAGCGACGACGGCGTCAAAATAATGGCGTAATCCCAGGTGCG                        <  SRR4302537.2749852/94‑1 (MQ=60)

                                                                       GCGCACAACAAAAATGTGTCTGACGCGGGTTTATGGTGTTTGACGTGATCGGCAGCGACGACGGCGTCAAAATAATGGCGTAATCCCAGGTGCG                        <  SRR4302537.5799891/94‑1 (MQ=60)

                                                                       GCGCACAACAAAAATGTGTCTGACGCGGGTTTATGGTGTTTGACGTGATCGGCAGCGACGACGGCGTCAAAATAATGGCGTAATCCCAGGTGCG                        >  SRR4302537.5058885/1‑94 (MQ=60)

                                                                         GCACAACAAAAATGTGTCTGACGCGGGTTTATGGTGTTTGACGTGATCGGCAGCGACGACGGCGTCAAAATAATGGCGTAATCCCAGGTGCGCC                      <  SRR4302537.1958444/94‑1 (MQ=60)

                                                                         GCACAACAAAAATGTGTCTGACGCGGGGTTATGGTGTTTGACGTGATCGGCAGCGACGACAGCGTCAAAATAATGGCGTAATCCCAGGTGCGCA                      >  SRR4302537.1798504/1‑94 (MQ=60)

                                                                         GCACAACAAAAATGTGTCTGACGCGGGCTTATGGTGTTTGACGTGATCGGCAGCCACGACGGCGACAAAATAATGGCGTAATCCCAGGTGCGCC                      >  SRR4302537.6391385/1‑94 (MQ=60)

                                                                          CACAGCAAAAATGTGTCTGACGCGGGTTTATGGTGTTTGACGTGATCGGCAGCGACGACGGCGTCAAAATAATGGCGTAATCCCAGGTGCGCCA                     <  SRR4302537.41241/94‑1 (MQ=60)

                                                                           ACAACAAAAATGTGTCTGGCGCGGGTTTATGGTGTTTGACGTGATCGGCAGCGACGACGGCGTCAAAATAATGGCGTAATCCCAGGTGCGCCAG                    <  SRR4302537.3786851/94‑1 (MQ=60)

                                                                           ACAACAAAAATGTGTCTGACGCGGGTTTATGGTGTTTGACGTGATCGGCAGCGACGACGGCGTCAAAATAATGGCGTAATCCCAGGTGCGCCAG                    <  SRR4302537.4156474/94‑1 (MQ=60)

                                                                           ACAACAAAAATGTGTCTGACGCGGGTTTATGGTGTTTGACGTGATCGGCAGCGACGACGGCGTCAAAATAATGGCGTAATCCCAGGTGCGCCAG                    >  SRR4302537.2926927/1‑94 (MQ=60)

                                                                           ACAACAAAAATGTGTCTGACGCGGGTTTATGGTGTTTGACGTGATCGGCAGCGACGACGGCGTCAAAATAATGGCGTAATCCCAGGTGCGCCAG                    >  SRR4302537.6098245/1‑94 (MQ=60)

                                                                             AACAAAAATGTGTCTGACGCGGGTTTATGGTGTTTGACGTGATCGGCAGCGACGACGGCGTCAAAATAATGGCGTAATCCCAGGTGCGCCAGCA                  >  SRR4302537.2201453/1‑94 (MQ=60)

                                                                              ACAAAAATGTGTCTGACGCGGGTTTATGGTGTTTGACGTGATCGGCAGCGACGACGGCGTCAAAATAATGGCGTAATCCCAGGTGCGCCAGCCA                 >  SRR4302537.4048191/1‑94 (MQ=60)

                                                                              ACAAAAATGTGTCTGACGCGGGTTTATGGTGTTTGACGTGATCGGCAGCGACGACGGCGTCAAAATAATGGCGTAATCCCAGGTGCGCCAGCAA                 >  SRR4302537.385129/1‑94 (MQ=60)

                                                                               CAAAAATGTGTCTGACGCGGGTTTATGGTGTTTGACGTGATCGGCAGCGACGACGGCGTCAAAATAATGGCGTAATCCCAGGTGCGCCAGCAAT                <  SRR4302537.681883/94‑1 (MQ=60)

                                                                               CAAAAATGTGTCTGACGCGGGTTTATGGTGTTTGACGTGATCGGCAGCGACGACGGCGTCAAAATAATGGCGTAATCCCAGGTGCGCCAGCAAT                <  SRR4302537.1193217/94‑1 (MQ=60)

                                                                                AAAAATGTGTCTGACGCGGGTTTATGGTGTTTGACGTGATCGGCAGCGACGACGGCGTCCAAATAATGGCGTAATCCCAGGTGCGCCAGCAATG               >  SRR4302537.3681820/1‑94 (MQ=60)

                                                                                AAAAATGTGTCTGACGCGGGTTTATGGTGTTTGACGTGATCGGCAGCGACGACGGCGTCAAAATAATGGCGTAATCCCAGGTGCGCCAGCAATG               >  SRR4302537.1117586/1‑94 (MQ=60)

                                                                                AAAAATGTGTCTGACGCGGGTTTATGGTGTTTGACGTGATCGGCAGCGACGACGGCGTCAAAATAATGGCGTAATCCCAGGTGCGCCAGCAATG               >  SRR4302537.5283814/1‑94 (MQ=60)

                                                                                 AAAATGTGTCTGACGCGGGTTTATGGTGTTTGACGTGATCGGCAGCGACGACGGCGTCAAAATAATGGCGTAATCCCAGGTGCGCCAGCAATGC              <  SRR4302537.5884168/94‑1 (MQ=60)

                                                                                  AAATGTGTCTGACGCGGGTTTCTGGTGTTTGACGTGATCGGCAGCGACGACGGCGTCAAAATCATGGAGTAATACCAGGTGCGCaatcaatgac             >  SRR4302537.604106/1‑84 (MQ=60)

                                                                                  AAATGTGTCTGACGCGGGTTTATGGTGTTTGACGTGATCGGCAGCGACGACGGCGTCAAAATAATGGCGTAATCCCAGGTGCGCCAGCAATGCC             <  SRR4302537.6089265/94‑1 (MQ=60)

                                                                                  AAATGTGTCTGACGCGGGTTTATGGTGTTTGACGTGATCGGCAGCGACGACGGCGTCAAAATAATGGCGTAATCCCAGGTGCGCCAGCAATGCC             <  SRR4302537.6238892/94‑1 (MQ=60)

                                                                                  AAATGTGTCTGACGCGGGTTTATGGTGTTTGACGTGATCGGCAGCGACGACGGCGTCAAAATAATGGCGTAATCCCAGGTGCGCCAGCAATGCC             <  SRR4302537.2127665/94‑1 (MQ=60)

                                                                                   AATGTGTCTGACGCGGGTTTATGGTGTTTGACGTGATCGGCAGCGACGACGGCGTCAAAATAATGGCGTAATCCCAGGTGCGCCAGCAATGCCT            >  SRR4302537.2378387/1‑94 (MQ=60)

                                                                                   AATGTGTCTGACGCGGGTTTATGGTGTTTGACGTGATCGGCAGCGACGACGGCGTCAAAATAATGGCGTAATCCCAGGTGCGCCAGCAATGCCT            <  SRR4302537.4557181/94‑1 (MQ=60)

                                                                                   AATGTGTCTGACGCGGGTTTATGGTGTTTGACGTGATCGGCAGCGACGACGGCGTCAAAATAATGGCGTAATCCCAGGTGCGCCAGCAATGCCT            >  SRR4302537.3530618/1‑94 (MQ=60)

                                                                                   AATGTGTCTGACGCGGGTTTATGGTGTTTGACGTGATCGGCAGCGACGACGGCGTCAAAATAATGGCGTAATCCCAGGTGCGCCAGCAATGCCT            >  SRR4302537.6504419/1‑94 (MQ=60)

                                                                                    ATGTGTCTGACGCGGGTTTATGGTGTTTGACGTGATCGGCAGCGACGACGGCGTCAAAATAATGGCGTAATCCCAGGTGCGCCAGCAATGCCTC           >  SRR4302537.5939744/1‑94 (MQ=60)

                                                                                    ATGTGTCTGACGCGGGTTTATGGTGTTTGACGTGATCGGCAGCGACGACGGCGTCAAAATAATGGCGTAATCCCAGGTGCGCCAGCAATGCCTC           >  SRR4302537.2683515/1‑94 (MQ=60)

                                                                                      GTGTCTGACGCGGGTTTATGGTGTTTGACGTGATCGGCAGCGACGACGGCGTCAAAATAATGGCGTAATCCCAGGTGCGCCAGCAATGCCTCAG         >  SRR4302537.5781378/1‑94 (MQ=60)

                                                                                      GTGTCTGACGCGGGTTTATGGTGTTTGACGTGATCGGCAGCGACGACGGCGTCAAAATAATGGCGTAATCCCAGGTGCGCCAGCAATGCCTCAG         >  SRR4302537.4548778/1‑94 (MQ=60)

                                                                                      GTGTCTGACGCGGGTTTATGGTGTTTGACGTGATCGGCAGCGACGACGGCGTCAAAATAATGGCGTAATCCCAGGTGCGCCAGCAATGCCTCAG         >  SRR4302537.4163182/1‑94 (MQ=60)

                                                                                      GTGTCTGACGCGGGTTTATGGTGTTTGACGTGATCGGCAGCGACGACGGCGTCAAAATAATGGCGTAATCCCAGGTGCGCCAGCAATGCCTCAG         >  SRR4302537.2128379/1‑94 (MQ=60)

                                                                                      GTGTCTGACGCGGGTTTATGGTGTTTGACGTGATCGGCAGCGACGACGGCGTCAAAATAATGGCGTAATCCCAGGTGCGCCAGCAATGCCTCAG         >  SRR4302537.6089816/1‑94 (MQ=60)

                                                                                      GTGTCTGACGCGGGTTTATGGTGTTTGACGTGATCGGAAGCGACGACGGCGTCAAAATAATGGCGTAATCCCAGGTGCGCCAGCAATGCCTCAG         <  SRR4302537.4155378/94‑1 (MQ=60)

                                                                                       TGTCTGACGCGGGTTTATGGTGTTTGACGTGATCGGCAGCGACGACGGCGTCAAAATAATGGCGTAATCCCAGGTGCGCCAGCAATGCCTCAGC        <  SRR4302537.716830/94‑1 (MQ=60)

                                                                                        GTCTGACGCGGGTTTATGGTGTTTGACGTGATCGGCAGCGACGACGGCGTCAAAATAATGGCGTAATCCCAGGTGCGCCAGCAATGCCTCAGCG       <  SRR4302537.2262075/94‑1 (MQ=60)

                                                                                        GTCTGACGCGGGTTTATGGTGTTTGACGTGATCGGCAGCGACGACGGCGTCAAAATAATGGCGTAATCCCAGGTGCGCCAGCAATGCCTCAGCG       <  SRR4302537.2343149/94‑1 (MQ=60)

                                                                                            GACGCGGGTTTATGGTGTTTGACGTGATCGGCAGCGACGACGGCGTCAAAATAATGGCGTAATCCCAGGTGCGCCAGCAATGCCTCAGCGATGG   <  SRR4302537.5013910/94‑1 (MQ=60)

                                                                                             ACGCGGGTTTATGGTGTTTGACGTGATCGGCAGCGACGACGGCGTCAAAATAATGGCGTAATTCCAGGTGCGCCAGCAATGCCTCAGCGATGGC  >  SRR4302537.5485630/1‑94 (MQ=60)

GGGCCGCCTGAATACCGAAATCGGCATCTTCAAAGACCACACACTGCGTCGGTTGCACGCCCATACGCTGCGCGCACAACAAAAATGTGTCTGGCGCGGGTTTATGGTGTTTGACGTGATCGGCAGCGACGACGGCGTCAAAATAATGGCGTAATCCCAGGTGCGCCAGCAATGCCTCAGCGATGGC  >  NC_000913/2815000‑2815186

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 0 ≤` `ATCG/ATCG` `< 2 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`