Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I2 R1 882 254.4 12957588 98.0% 12698436 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,823,915 A→G K21E (AAG→GAG)  srlA → PTS system, glucitol/sorbitol‑specific IIC component

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,823,9150AG100.0% 769.3 / NA 220K21E (AAG→GAG) srlAPTS system, glucitol/sorbitol‑specific IIC component
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (90/130);  total (90/130)

Only 100 of 229 total aligned reads displayed.

ATAAAACAATAAATCCTGAAGGAGAGAACAATGATAGAAACCATTACTCATGGTGCAGAGTGGTTTATCGGGCTGTTCCAAAAGGGCGGAAAGGTGTTTACCGGGATGGTGACCGGCATTCTTCCGCTGTTGATTAGCCTGCTGGTTATCATGAACGCACTGATTAATTTTATCGGTCAG  >  NC_000913/2823825‑2824004
                                                                                          |                                                                                         
 taaAACAATAAATCCTGAAGGAGAGAACAATGATAGAAACCATTACTCATGGTGCAGAGTGGTTTATCGGGCTGTTCCAAAAGGGCGGAGAgg                                                                                        >  1:4981993/1‑93 (MQ=255)
 taaAACAATAAATCCTGAAGGAGAGAACAATGATAGAAACCATTACTCATGGTGCAGAGTGGTTTATCGGGCTGTTCCAAAAGGGCGGAGAgg                                                                                        >  1:6074982/1‑93 (MQ=255)
 taaAACAATAAATCCTGAAGGAGAGAACAATGATAGAAACCATTACTCATGGTGCAGAGTGGTTTATCGGGCTGTTCCAAAAGGGCGGAGAgg                                                                                        >  1:2037386/1‑93 (MQ=255)
  aaaaCAATAAATCCTGAAGGAGAGAACAATGATAGAAACCATTACTCATGGTGCAGAGTGGTTTATCGGGCTGTTCCAAAAGGGCGGAGAGgt                                                                                       <  2:1774031/93‑1 (MQ=255)
     aCAATAAATCCTGAAGGAGAGAACAATGATAGAAACCATTACTCATGGTGCAGAGTGGTTTATCGGGCTGTTCCAAAAGGGCGGAGAGGTGtt                                                                                    >  2:1456469/1‑93 (MQ=255)
      cAATAAATCCTGAAGGAGAGAACAATGATAGAAACCATTACTCATGGTGCAGAGTGGTTTATCGGGCTGTTCCAAAAGGGCGGAGAGGTGttt                                                                                   <  2:2735302/93‑1 (MQ=255)
       aaTAAATCCTGAAGGAGAGAACAATGATAGAAACCATTACTCATGGTGCAGAGTGGTTTATCGGGCTGTTCCAAAAGGGCGGAGAGGTGTTTa                                                                                  >  1:2414532/1‑93 (MQ=255)
       aaTAAATCCTGAAGGAGAGAACAATGATAGAAACCATTACTCATGGTGCAGAGTGGTTTATCGGGCTGTTCCAAAAGGGCGGAGAGGTGTTTa                                                                                  <  2:1978311/93‑1 (MQ=255)
        aTAAATCCTGAAGGAGAGAACAATGATAGAAACCATTACTCATGGTGCAGAGTGGTTTATCGGGCTGTTCCAAAAGGGCGGAGAGGTGTTTAc                                                                                 <  2:6227484/93‑1 (MQ=255)
          aaaTCCTGAAGGAGAGAACAATGATAGAAACCATTACTCATGGTGCAGAGTGGTTTATCGGGCTGTTCCAAAAGGGCGGAGAGGTGTTTACCg                                                                               <  2:5912463/93‑1 (MQ=255)
             tCCTGAAGGAGAGAACAATGATAGAAACCATTACTCATGGTGCAGAGTGGTTTATCGGGCTGTTCCAAAAGGGCGGAGAGGTGTTTACCGGGa                                                                            >  1:1098150/1‑93 (MQ=255)
              ccTGAAGGAGAGAACAATGATAGAAACCATTGCTCATGGTGCAGAGTGGTTTATCGGGCTGTTCCAAAAGGGCGGAGAGGTGTTTACCGGGAt                                                                           <  1:6333096/93‑1 (MQ=255)
               cTGAAGGAGAGAACAATGATAGAAACCATTACTCATGGTGCAGAGTGGTTTATCGGGCTGTTCCAAAAGGGCGGAGAGGTGTTTACCGGGATg                                                                          >  2:2915891/1‑93 (MQ=255)
               cTGAAGGAGAGAACAATGATAGAAACCATTACTCATGGTGCAGAGTGGTTTATCGGGCTGTTCCAAAAGGGCGGAGAGGTGTTTACCGGGATg                                                                          >  1:209981/1‑93 (MQ=255)
               cTGAAGGAGAGAACAATGATAGAAACCATTACTCATGGTGCAGAGTGGTTTATCGGGCTGTTCCAAAAGGGCGGAGAGGTGTTTACCGGGATg                                                                          <  2:443762/93‑1 (MQ=255)
                  aaGGAGAGAACAATGATAGAAACCATTACTCATGGTGCAGAGTGGTTTATCGGGCTGTTCCAAAAGGGCGGAGAGGTGTTTACCGGGATGGTg                                                                       <  1:5831673/93‑1 (MQ=255)
                  aaGGAGAGAACAATGATAGAAACCATTACTCATGGTGCAGAGTGGTTTATCGGGCTGTTCCAAAAGGGCGGAGAGGTGTTTACCGGGATGGTg                                                                       >  1:3551166/1‑93 (MQ=255)
                    ggAGAGAACAATGATAGAAACCATTACTCATGGTGCAGAGTGGTTTATCGGGCTGTTCCAAAAGGGCGGAGAGGTGTTTACCGGGATGGTGAc                                                                     <  2:3505201/93‑1 (MQ=255)
                    ggAGAGAACAATGATAGAAACCATTACTCATGGTGCAGAGTGGTTTATCGGGCTGTTCCAAAAGGGCGGAGAGGTGTTTACCGGGATGGTGAc                                                                     <  2:914599/93‑1 (MQ=255)
                     gagagaACAATGATAGAAACCATTACTCATGGTGCAGAGTGGTTTATCGGGCTGTTCCAAAAGGGCGGAGAGGTGCTTAACGGGATAGAGAcc                                                                    >  2:33345/1‑93 (MQ=255)
                      agagaACAATGATAGAAACCATTACTCATGGTGCAGAGTGGTTTATCGGGCTGTTCCAAAAGGGCGGAGAGGTGTTTACCGGGATGGTGACCg                                                                   <  1:2670773/93‑1 (MQ=255)
                      agagaACAATGATAGAAACCATTACTCATGGTGCAGAGTGGTTTATCGGGCTGTTCCAAAAGGGCGGAGAGGAGTTTACCGGGATGGTGACCg                                                                   >  2:3139962/1‑93 (MQ=255)
                        agaACAATGATAGAAACCATTACTCATGGTGCAGAGTGGTTTATCGGGCTGTTCCAAAAGGGCGGAGAGGTGTTTACCGGGATGGTGACCGGc                                                                 <  1:54724/93‑1 (MQ=255)
                        agaACAATGATAGAAACCATTACTCATGGTGCAGAGTGGTTTATCGGGCTGTTCCAAAAGGGCGGAGAGGTGTTTACCGGGATGGTGACCGGc                                                                 <  2:1244198/93‑1 (MQ=255)
                        agaACAATGATAGAAACCATTACTCATGGTGCAGAGTGGTTTATCGGGCTGTTCCAAAAGGGCGGAGAGGTGTTTACCGGGATGGTGACCGGc                                                                 >  2:1151822/1‑93 (MQ=255)
                         gaACAATGATAGAAACCATTACTCATGGTGCAGAGTGGTTTATCGGGCTGTTCCAAAAGGGCGGAGAGGTGTTTACCGGGATGGTGACCGGCa                                                                >  2:3038047/1‑93 (MQ=255)
                         gaACAATGATAGAAACCATTACTCATGGTGCAGAGTGGTTTATCGGGCTGTTCCAAAAGGGCGGAGAGGTGTTTACCGGGATGGTGACCGGCa                                                                <  2:2623261/93‑1 (MQ=255)
                         gaACAATGATAGAAACCATTACTCATGGTGCAGAGTGGTTTATCGGGCTGTTCCAAAAGGGCGGAGAGGTGTTTACCGGGATGGTGACCGGCa                                                                <  1:3957749/93‑1 (MQ=255)
                           aCAATGATAGAAACCATTACTCATGGTGCAGAGTGGTTTATCGGGCTGTTCCAAAAGGGCGGAGAGGTGTTTACCGGGATGGTGACCGGCAtt                                                              >  1:1408118/1‑93 (MQ=255)
                            cAATGATAGAAACCATTACTCATGGTGCAGAGTGGTTTATCGGGCTGTTCCAAAAGGGCGGAGAGGTGTTTACCGGGATGGTGACCGGCAttc                                                             >  2:2846855/1‑93 (MQ=255)
                            cAATGATAGAAACCATTACTCATGGTGCAGAGTGGTTTATCGGGCTGTTCCAAAAGGGCGGAGAGGTGTTTACCGGGATGGTGACCGGCAttc                                                             >  1:6133299/1‑93 (MQ=255)
                             aaTGATAGAAACCATTACTCATGGTGCAGAGTGGTTTATCGGGCTGTTCCAAAAGGGCGGAGAGGTGTTTACCGGGATGGTGACCGGCAttct                                                            >  1:1299876/1‑93 (MQ=255)
                             aaTGATAGAAACCATTACTCATGGTGCAGAGTGGTTTATCGGGCTGTTCCAAAAGGGCGGAGAGGTGTTTACCGGGATGGTGACCGGCAttct                                                            <  1:2173202/93‑1 (MQ=255)
                              aTGATAGAAACCATTACTCATGGTGCAGAGTGGTTTATCGGGCTGTTCCAAAAGGGCGGAGAGGTGTTTACCGGGATGGTGACCGGCAttctt                                                           >  2:5054667/1‑93 (MQ=255)
                              aTGATAGAAACCATTACTCATGGTGCAGAGTGGTTTATCGGGCTGTTCCAAAAGGGCGGAGAGGTGTTTACCGGGATGGTGACCGGCAttctt                                                           >  2:1729956/1‑93 (MQ=255)
                               tGATAGAAGCCATTACTCATGGTGCAGAGTTGTTTATCGGGCTGTTCCAAAAGGGCGGAGAGGTGTTTACCGGGATGGTGACCGGCAttcttc                                                          <  2:5370800/93‑1 (MQ=255)
                               tGATAGAAACCATTACTCATGGTGCAGAGTGGTTTAACGGGCTGTTCCAAAAGGGCGGAGAGGTGTTTACCGGGATGGTGACCGGCAttcttc                                                          >  2:321510/1‑93 (MQ=255)
                                 aTAGAAACCATTACTCATGGTGCAGAGTGGTTTATCGGGCTGTTCCAAAAGGGCGGAGAGGTGTTTACCGGGATGGTGACCGGCATTCTTCCg                                                        >  2:568857/1‑93 (MQ=255)
                                  tAGAAACCATTACTCATGGTGCAGAGTGGTTTATCGGGCTGTTCCAAAAGGGCGGAGAGGTGTTTACCGGGATGGTGACCGGCATTCTTCCGc                                                       >  1:2857187/1‑93 (MQ=255)
                                   aGAAACCATTACTCATGGTGCAGAGTGGTTTATCGGGCTGTTCCAAAAGGGCGGAGAGGTGTTTACCGGGATGGTGACCGGCATTCTTCCGCt                                                      <  1:5572431/93‑1 (MQ=255)
                                    gAAACCATTACTCATGGTGCAGAGTGGTTTATCGGGCTGTTCCAAAAGGGCGGAGAGGTGTTTACCGGGATGGTGACCGGCATTCTTCCGCTg                                                     <  2:823673/93‑1 (MQ=255)
                                     aaaCCATTACTCATGGTGCAGAGTGGTTTATCGGGCTGTTCCAAAAGGGCGGAGAGGTGTTTACCGGGATGGTGACCGGCATTCTTCCGCTGt                                                    >  2:3448800/1‑93 (MQ=255)
                                      aaCCATTACTCATGGTGCAGAGTGGTTTATCGGGCTGTTCCAAAAGGGCGGAGAGGTGTTTACCGGGATGGTGACCGGCATTCTTCCGCTGtt                                                   <  1:4589430/93‑1 (MQ=255)
                                      aaCCATTACTCATGGTGCAGAGTGGTTTATCGGGCTGTTCCAAAAGGGCGGAGAGGTGTTTACCGGGATGGTGACCGGCATTCTTCCGCTGtt                                                   <  2:2222082/93‑1 (MQ=255)
                                       aCCATTACTCATGGTGCAGAGTGGTTTATCGGGCTGTTCCAAAAGGGCGGAGAGGTGTTTACCGGGATGGTGACCGGCATTCTTCCGCTGTTg                                                  >  1:4868969/1‑93 (MQ=255)
                                       aCCATTACTCATGGTGCAGAGTGGTTTATCGGGCTGTTCCAAAAGGGCGGAGAGGTGTTTACCGGGATGGTGACCGGCATTCTTCCGCTGTTg                                                  >  1:4139165/1‑93 (MQ=255)
                                       aCCATTACTCATGGTGCAGAGTGGTTTATCGGGCTGTTCCAAAAGGGCGGAGAGGTGTTTACCGGGATGGTGACCGGCATTCTTCCGCTGTTg                                                  >  1:2530147/1‑93 (MQ=255)
                                           ttACTCATGGTGCAGAGTGGTTTATCGGGCTGTTCCAAAAGGGCGGAGAGGTGTTTACCGGGATGGTGACCGGCATTCTTCCGCTGTTGATTa                                              <  2:5115906/93‑1 (MQ=255)
                                           ttACTCATGGTGCAGAGTGGTTTATCGGGCTGTTCCAAAAGGGCGGAGAGGTGTTTACCGGGATGGTGACCGGCATTCTTCCGCTGTTGATTa                                              <  1:3559105/93‑1 (MQ=255)
                                                cATGGTGCAGAGTGGTTTATCGGGCTGTTCCAAAAGGGCGGAGAGGTGTTTACCGGGATGGTGACCGGCATTCTTCCGCTGTTGATTAGCctg                                         <  2:3393680/93‑1 (MQ=255)
                                                 aTGGTGCAGAGTGGTTTATCGGGCTGTTCCAAAAGGGCGGAGAGGTGTTTACCGGGATGGTGACCGGCATTCTTCCGCTGTTGATTAGCctgc                                        >  2:4359215/1‑93 (MQ=255)
                                                 aTGGTGCAGAGTGGTTTATCGGGCTGTTCCAAAAGGGCGGAGAGGTGTTTACCGGGATGGTGACCGGCATTCTTCCGCTGTTGATTAGCctgc                                        >  2:1938854/1‑93 (MQ=255)
                                                  tGGTGCAGAGTGGTTTATCGGGCTGTTCCAAAAGGGCGGAGAGGTGTTTACCGGGATGGTGACCGGCATTCTTCCGCTGTTGATTAGCctgct                                       <  1:5979960/93‑1 (MQ=255)
                                                   ggTGCAGAGTGGTTTATCGGGCTGTTCCAAAAGGGCGGAGAGGTGTTTACCGGGATGGTGACCGGCATTCTTCCGCTGTTGATTAGCctgctg                                      <  1:1006999/93‑1 (MQ=255)
                                                   ggTGCAGAGTGGTTTATCGGGCTGTTCCAAAAGGGCGGAGAGGTGTTTACCGGGATGGTGACCGGCATTCTTCCGCTGTTGATTAGCctgctg                                      <  1:3751483/93‑1 (MQ=255)
                                                    gTGCAGAGTGGTTTATCGGGCTGTTCCAAAAGGGCGGAGAGGTGTTTACCGGGATGGTGACCGGCATTCTTCCGCTGTTGATTAGCCTGCTgg                                     <  2:4976070/93‑1 (MQ=255)
                                                       cAGAGTGGTTTATCGGGCTGTTCCAAAAGGGCGGAGAGGTGTTTACCGGGATGGTGACCGGCATTCTTCCGCTGTTGATTAGCCTGCTGGTTa                                  <  2:1360344/93‑1 (MQ=255)
                                                        agagTGGTTTATCGGGCTGTTCCAAAAGGGCGGAGAGGTGTTTACCGGGATGGTGACCGGCATTCTTCCGCTGTTGATTAGCCTGCTGGTTAt                                 <  2:6074982/93‑1 (MQ=255)
                                                         gagTGGTTTATCGGGCTGTTCCAAAAGGGCGGAGAGGTGTTTACCGGGATGGTGACCGGCATTCTTCCGCTGTTGATTAGCCTGCTGGTTATc                                <  1:2288671/93‑1 (MQ=255)
                                                         gagTGGTTTATCGGGCTGTTCCAAAAGGGCGGAGAGGTGTTTACCGGGATGGTGACCGGCATTCTTCCGCTGTTGATTAGCCTGCTGGTTATc                                >  1:5310475/1‑93 (MQ=255)
                                                         gagTGGTTTATCGGGCTGTTCCAAAAGGGCGGAGAGGTGTTTACCGGGATGGTGACCGGCATTCTTCCGCTGTTGATTAGCCTGCTGGTTATc                                <  1:5105210/93‑1 (MQ=255)
                                                         gagTGGTTTATCGGGCTGTTCCAAAAGGGCGGAGAGGTGTTTACCGGGATGGTGACCGGCATTCTTCCGCTGTTGATTAGCCTGCTGGTTATc                                <  1:1702800/93‑1 (MQ=255)
                                                         gagTGGTTTATCGGGCTGTTCCAAAAGGGCGGAGAGGTGTTTACCGGGATGGTGACCGGCATTCTTCCGCTGTTGATTAGCCTGCTGGTTATc                                <  1:2985862/93‑1 (MQ=255)
                                                           gTGGTTTATCGGGCTGTTCCAAAAGGGCGGAGAGGGGTTTACCGGGATGGTGACCGGCATTCTTCCGCTGTTGATTAGCCTGCTGGTTATCAt                              >  1:3078422/1‑93 (MQ=255)
                                                             ggTTTATCGGGCTGTTCCAAAAGGGCGGAGAGGTGTTTACCGGGATGGTGACCGGCATTCTTCCGCTGTTGATTAGCCTGCTGGTTATCATGa                            <  1:4317072/93‑1 (MQ=255)
                                                              gTTTATCGGGCTGTTCCAAAAGGGCGGAGAGGTGTTTACCGGGATGGTGACCGGCATTCTTCCGCTGTTGATTAGCCTGCTGGTTATCATGaa                           >  2:4005433/1‑93 (MQ=255)
                                                              gTTTATCGGGCTGTTCCAAAAGGGCGGAGAGGTGTTTACCGGGATGGTGACCGGCATTCTTCCGCTGTTGATTAGCCTGCTGGTTATCATGaa                           >  1:3289609/1‑93 (MQ=255)
                                                              gTTTATCGGGCTGTTCCAAAAGGGCGGAGAGGTGTTTACCGGGATGGTGACCGGCATTCTTCCGCTGTTGATTAGCCTGCTGGTTATCATGaa                           <  2:5541534/93‑1 (MQ=255)
                                                              gTTTATCGGGCTGTTCCAAAAGGGCGGAGAGGTGTTTACCGGGATGGTGACCGGCATTCTTCCGCTGTTGATTAGCCTGCTGGTTATCATGaa                           >  1:6324440/1‑93 (MQ=255)
                                                              gTTTATCGGGCTGTTCCAAAAGGGCGGAGAGGTGTTTACCGGGATGGTGACCGGCATTCTTCCGCTGTTGATTAGCCTGCTGGTTATCATGaa                           >  2:1584622/1‑93 (MQ=255)
                                                               tttATCGGGCTGTTCCAAAAGGGCGGAGAGGTGTTTACCGGGATGGTGACCGGCATTCTTCCGCTGTTGATTAGCCTGCTGGTTATCATGAAc                          <  2:2683340/93‑1 (MQ=255)
                                                               tttATCGGGCTGTTCCAAAAGGGCGGAGAGGTGTTTACCGGGATGGTGACCGGCATTCTTCCGCTGTTGATTAGCCTGCTGGTTATCATGAAc                          <  1:991060/93‑1 (MQ=255)
                                                                    cGGGCTGTTCCAAAAGGGCGGAGAGGTGTTTACCGGGATGGTGACCGGCATTCTTCCGCTGTTGATTAGCCTGCTGGTTATCATGAACGCACt                     <  2:491661/93‑1 (MQ=255)
                                                                      ggCTGTTCCAAAAGGGCGGAGAGGTGTTTACCGGGATGGTGACCGGCATTCTTCCGCTGTTGATTAGCCTGCTGGTTATCATGAACGCACTGa                   <  1:3145162/93‑1 (MQ=255)
                                                                       gCTGTTCCAAAAGGGCGGAGAGGTTTTTACCGGGATGGTGACCGGCATTCTTCCGCTGTTGATTAGCCTGCTGGTTATCATGAAAGCACTGAt                  <  2:6290153/93‑1 (MQ=255)
                                                                       gCTGTTCCAAAAGGGCGGAGAGGTGCTTACCGGGATGGTGACCGGCATTCTTCCGCTGTTGATTAGCCTGCTGGTTATCATGAACGCACTGAt                  <  2:2880527/93‑1 (MQ=255)
                                                                        cTGTTCCAAAAGGGCGGAGAGGTGTTTACCGGGATGGTGACCGGCATTCTTCCGCTGTTGATTAGCCTGCTGGTTATCATGAACGCACTGAtt                 <  2:3917829/93‑1 (MQ=255)
                                                                         tGTTCCAAAAGGGCGGAGAGGTGTTTACCGGGATGGTGACCGGCATTCTTCCGCTGTTGATTAGCCTGCTGGTTATCATGAACGCACTGATTa                >  2:2424748/1‑93 (MQ=255)
                                                                         tGTTCCAAAAGGGCGGAGAGGTGTTTACCGGGATGGTGACCGGCATTCTTCCGCTGTTGATTAGCCTGCTGGTTATCATGAACGCACTGATTa                >  1:5217572/1‑93 (MQ=255)
                                                                         tGTTCCAAAAGGGCGGAGAGGTGTTTACCGGGATGGTGACCGGCATTCTTCCGCTGTTGATTAGCCTGCTGGTTATCATGAACGCACTGATTa                >  1:280226/1‑93 (MQ=255)
                                                                           ttCGAAAAGGGCGGAGAGGTGTTTACCGGGATGGTGACCGGCATTCTTCCGCTGTTGATTAGCCTGCTGGTTCTCATGAACGCACTGATTAAt              <  2:4938432/93‑1 (MQ=255)
                                                                           ttCCAAAAGGGCGGAGAGGTGTTTACCGGGATGGTGACCGGCATTCTTCCGCTGTTGATTAGCCTGCTGGTTATCATGAACGCACTGATTAAt              >  2:2415560/1‑93 (MQ=255)
                                                                           ttCCAAAAGGGCGGAGAGGTGTTTACCGGGATGGTGACCGGCATTCTTCCGCTGTTGATTAGCCTGCTGGTTATCATGAACGCACTGATTAAt              >  1:833880/1‑93 (MQ=255)
                                                                            tcgaaaaGGGCGGAGAGGTGTGTACCGGGAGGGTGACCGGCATGCTTCCGCTGTTGATTAGCCTGCTGGTTATCATGAACGCACTGATTAAtt             <  2:4512014/90‑1 (MQ=255)
                                                                            tCCAAAAGGGCGGAGAGGTGTTTACCGGGATGGTGACCGGCATTCTTCCGCTGTTGATTAGCCTGCTGGTTATCATGAACGCACTGATTAAtt             <  1:616264/93‑1 (MQ=255)
                                                                            tCCAAAAGGGCGGAGAGGTGTTTACCGGGATGGTGACCGGCATTCTTCCGCTGTTGATTAGCCTGCTGGTTATCATGAACGCACTGATTAAtt             <  1:4952811/93‑1 (MQ=255)
                                                                              cAAAAGGGCGGAGAGGTGTTTACCGGGATGGTGACCGGCATTCTTCCGCTGTTGATTAGCCTGCTGGTTATCATGAACGCACTGATTAAtttt           >  2:3739271/1‑93 (MQ=255)
                                                                              cAAAAGGGCGGAGAGGTGTTTACCGGGATGGTGACCGGCATTCTTCCGCTGTTGATTAGCCTGCTGGTTATCATGAACGCACTGATTAAtttt           >  1:4278792/1‑93 (MQ=255)
                                                                               aaaaGGGCGGAGAGGTGTTTACCGGGATGGTGACCGGCATTCTTCCGCTGTTGATTAGCCTGCTGGTTATCATGAACGCACTGATTAATTTTa          <  1:2234482/93‑1 (MQ=255)
                                                                               aaaaGGGCGGAGAGGTGTTTACCGGGATGGTGACCGGCATTCTTCCGCTGTTGATTAGCCTGCTGGTTATCATGAACGCACTGATTAATTTTa          >  2:3714673/1‑93 (MQ=255)
                                                                                  aGGGCGGAGAGGTGTTTACCGGGATGGTGACCGGCATTCTTCCGCTGTTGATTAGCCTGCTGGTTATCATGAACGCACTGATTAATTTTATCg       >  2:1119359/1‑93 (MQ=255)
                                                                                   gggCGGAGAGGTGTTTACCGGGATGGTGACCGGCATTCTTCCGCTGTTGATTAGCCTGCTGGTTATCATGAACGCACTGATTAATTTTATCgg      <  1:3809618/93‑1 (MQ=255)
                                                                                    ggCGGAGAGGTGTTTACCGGGATGGTGACCGGCATTCTTCCGCTGTTGATTAGCCTGCTGGTTATCATGAACGCACTGATTAATTTTATCGGt     <  2:1951625/93‑1 (MQ=255)
                                                                                    ggCGGAGAGGTGTTTACCGGGATGGTGACCGGCATTCTTCCGCTGTTGATTAGCCTGCTGGTTATCATGAACGCACTGATTAATTTTATCGGt     <  1:2880903/93‑1 (MQ=255)
                                                                                     gCGGAGAGGTGTTTACCGGGATGGTGACCGGCATTCTTCCGCTGTTGATTAGCCTGCTGGTTATCATGAACGCACTGATTAATTTTATCGGTc    <  1:733443/93‑1 (MQ=255)
                                                                                     gCGGAGAGGTGTTTACCGGGATGGTGACCGGCATTCTTCCGCTGTTGATTAGCCTGCTGGTTATCATGAACGCACTGATTAATTTTATCGGTc    <  2:485165/93‑1 (MQ=255)
                                                                                     gCGGAGAGGTGTTTACCGGGATGGTGACCGGCATTCTTCCGCTGTTGATTAGCCTGCTGGTTATCATGAACGCACTGATTAATTTTATCGATc    <  2:5646804/93‑1 (MQ=255)
                                                                                      cGGAGAGGTGTTTACCGGGATGGTGACCGGCATTCTTCCGCTGTTGATTAGCCTGCTGGTTATCATGAACGCACTGATTAATTTTATCGGTCa   <  2:692361/93‑1 (MQ=255)
                                                                                      cGGAGAGGTGTTTACCGGGATGGTGACCGGCATTCTTCCGCTGTTGATTAGCCTGCTGGTTATCATGAACGCACTGATTAATTTTATCGGTCa   <  1:1904136/93‑1 (MQ=255)
                                                                                       ggAGAGGTGTTTACCGGGATGGTGACCGGCATTCTTCCGCTGTTGATTAGCCTGCTGGTTATCATGAACGCACTGATTAATTTTATCGGTCAg  <  2:2041726/93‑1 (MQ=255)
                                                                                          |                                                                                         
ATAAAACAATAAATCCTGAAGGAGAGAACAATGATAGAAACCATTACTCATGGTGCAGAGTGGTTTATCGGGCTGTTCCAAAAGGGCGGAAAGGTGTTTACCGGGATGGTGACCGGCATTCTTCCGCTGTTGATTAGCCTGCTGGTTATCATGAACGCACTGATTAATTTTATCGGTCAG  >  NC_000913/2823825‑2824004

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 23 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
GTAATAAAACAATAAATCCTGAAGGAGAGAACAATGATAGAAACCATTACTCATGGTGCAGAGTGGTTTATCGGGCTGTTCCAAAAGGGCGGAAAGGTGTTTACCGGGATGGTGACCGGCATTCTTCCGCTGTTGATTAGCCTGCTGGTTATCATGAACGCACTGATTAATTTTATCGGTCAGCATC  >  NC_000913/2823822‑2824008
                                                                                             |                                                                                             
GTAATAAAACAATAAATCCTGTAGGAGAGAACAATGATAGAAACCATTACTCATGGTGCAGAGTGGTTTATCGGGCTGTTCCAAAAGGGCGGAG                                                                                               >  SRR4302537.1035460/1‑94 (MQ=60)
GTAATAAAACAATAAATCCTGAAGGAGAGAACAATGATAGAAACCATTACTCATGGTGCAGAGTGGTTTATCGGGCTGTTCCAAAAGGGCGGAG                                                                                               >  SRR4302537.2585947/1‑94 (MQ=60)
GTAATAAAACAATAAATCCTGAAGGAGAGAACAATGATAGAAACCATTACTCATGGTGCAGAGTGGTTTATCGGGCTGTTCCAAAAGGGCGGAG                                                                                               <  SRR4302537.3261661/94‑1 (MQ=60)
GTAATAAAACAATAAATCCTGAAGGAGAGAACAATGATAGAAACCATTACTCATGGTGCAGAGTGGTTTATCGGGCTGTTCCAAAAGGGCGGAG                                                                                               <  SRR4302537.1484318/94‑1 (MQ=60)
  AATAAAACAATAAATCCTGAAGGAGAGAACAATGATAGAAACCATTACTCATGGTGCAGAGTGGTTTATCGGGCTGTTCCAAAAGGGCGGAGAG                                                                                             <  SRR4302537.2535306/94‑1 (MQ=60)
   ATAAAACAATAAATCCTGAAGGAGAGAACAATGATAGAAACCATTACTCATGGTGCAGAGTGGTTTATCGGGCTGTTCCAAAAGGGCGGAGAGG                                                                                            >  SRR4302537.2051591/1‑94 (MQ=60)
   ATAAAACAATAAATCCTGAAGGAGAGAACAATGATAGAAACCATTACTCATGGTGCAGAGTGGTTTATCGGGCTGTTCCAAAAGGGCGGAGAGG                                                                                            >  SRR4302537.6116732/1‑94 (MQ=60)
   ATAAAACAATAAATCCTGAAGGAGAGAACAATGATAGAAACCATTACTCATGGTGCAGAGTGGTTTATCGGGCTGTTCCAAAAGGGCGGAGAGG                                                                                            >  SRR4302537.5016330/1‑94 (MQ=60)
         CAATAAATCCTGAAGGAGAGAACAATGATAGAAACCATTACTCATGGTGCAGAGTGGTTTATCGGGCTGTTCCAAAAGGGCGGAGAGGTGTTTA                                                                                      >  SRR4302537.2431522/1‑94 (MQ=60)
            TAAATCCTGAAGGAGAGAACAATGATAGAAACCATTACTCATGGTGCAGAGTGGTTTATCGGGCTGTTCCAAAAGGGCGGAGAGGTGTTTACCG                                                                                   <  SRR4302537.5148693/94‑1 (MQ=60)
              AATCCTGAAGGAGAGAACAATGATAGAAACCATTACTCATGGTGCAGAGTGGTTTATCGGGCTGTTCCAAAAGGGCGGAGAGGTGTTTACCGGG                                                                                 <  SRR4302537.1698499/94‑1 (MQ=60)
              AATCCTGAAGGAGAGAACAATGATAGAAACCATTACTCATGGTGCAGAGTGGTTTATCGGGCTGTTCCAAAAGGGCGGAGAGGTGTTTACCGGG                                                                                 >  SRR4302537.5431440/1‑94 (MQ=60)
               ATCCTGAAGGAGAGAACAATGATAGAAACCATTACTCATGGTGCAGAGTGGTTTATCGGGCTGTTCCAAAAGGGCGGAGAGGTGTTTACCGGGA                                                                                >  SRR4302537.1105738/1‑94 (MQ=60)
                 CCTGAAGGAGAGAACAATGATAGAAACCATTGCTCATGGTGCAGAGTGGTTTATCGGGCTGTTCCAAAAGGGCGGAGAGGTGTTTACCGGGATG                                                                              <  SRR4302537.6376398/94‑1 (MQ=60)
                 CCTGAAGGAGAGAACAATGATAGAAACCATTACTCATGGTGCAGAGTGGTTTATCGGGCTGTTCCAAAAGGGCGGAGAGGTGTTTACCGGGATG                                                                              <  SRR4302537.291609/94‑1 (MQ=60)
                 CCTGAAGGAGAGAACAATGATAGAAACCATTACTCATGGTGCAGAGTGGTTTATCGGGCTGTTCCAAAAGGGCGGAGAGGTGTTTACCGGGATG                                                                              >  SRR4302537.211489/1‑94 (MQ=60)
                  CTGAAGGAGAGAACAATGATAGAAACCATTACTCATGGTGCAGAGTGGTTTATCGGGCTGTTCCAAAAGGGCGGAGAGGTGTTTACCGGGATGG                                                                             <  SRR4302537.4188177/94‑1 (MQ=60)
                  CTGAAGGAGAGAACAATGATAGAAACCATTACTCATGGTGCAGAGTGGTTTATCGGGCTGTTCCAAAAGGGCGGAGAGGTGTTTACCGGGATGG                                                                             <  SRR4302537.910091/94‑1 (MQ=60)
                   TGAAGGAGAGAACAATGATAGAAACCATTACTCATGGTGCAGAGTGGTTTATCGGGCTGTTCCAAAAGGGCGGAGAGGTGTTTACCGGGATGGT                                                                            <  SRR4302537.4927661/94‑1 (MQ=60)
                   TGAAGGAGAGAACAATGATAGAAACCATTACTCATGGTGCAGAGTGGTTTATCGGGCTGTTCCAAAAGGGCGGAGAGGTGTTTACCGGGATGGT                                                                            <  SRR4302537.1004077/94‑1 (MQ=60)
                    GAAGGAGAGAACAATGATAGAAACCATTACTCATGGTGCAGAGTGGTTTATCGGGCTGTTCCAAAAGGGCGGAGAGGTGTTTACCGGGATGGTG                                                                           <  SRR4302537.2469741/94‑1 (MQ=60)
                    GAAGGAGAGAACAATGATAGAAACCATTACTCATGGTGCAGAGTGGTTTATCGGGCTGTTCCAAAAGGGCGGAGAGGTGTTTACCGGGATGGTG                                                                           >  SRR4302537.3576245/1‑94 (MQ=60)
                     AAGGAGAGAACAATGATAGAAACCATTACTCATGGTGCAGAGTGGTTTATCGGGCTGTTCCAAAAGGGCGGAGAGGTGTTTACCGGGATGGTGA                                                                          <  SRR4302537.5871639/94‑1 (MQ=60)
                       GGAGAGAACAATGATAGAAACCATTACTCATGGTGCAGAGTGGTTTATCGGGCTGTTCCAAAAGGGCGGAGAGGTGTTTACCGGGATGGTGACC                                                                        <  SRR4302537.6197233/94‑1 (MQ=60)
                       GGAGAGAACAATGATAGAAACCATTACTCATGGTGCAGAGTGGTTTATCGGGCTGTTCCAAAAGGGCGGAGAGGTGTTTACCGGGATGGTGACC                                                                        >  SRR4302537.2269849/1‑94 (MQ=60)
                         AGAGAACAATGATAGAAACCATTACTCATGGTGCAGAGTGGTTTATCGGGCTGTTCCAAAAGGGCGGAGAGGTGTTTACCGGGATGGTGACCGG                                                                      <  SRR4302537.2689431/94‑1 (MQ=60)
                           AGAACAATGATAGAAACCATTACTCATGGTGCAGAGTGGTTTATCGGGCTGTTCCAAAAGGGCGGAGAGGTGTTTACCGGGATGGTGACCGGCA                                                                    <  SRR4302537.55128/94‑1 (MQ=60)
                            GAACAATGATAGAAACCATTACTCATGGTGCAGAGTGGTTTATCGGGCTGTTCCAAAAGGGCGGAGAGGTGTTTACCGGGATGGTGACCGGCAT                                                                   <  SRR4302537.3985443/94‑1 (MQ=60)
                             AACAATGATAGAAACCATTACTCATGGTGCAGAGTGGTTTATCGGGCTGTTCCAAAAGGGCGGAGAGGTGTTTACCGGGATGGTGACCGGCATT                                                                  >  SRR4302537.346552/1‑94 (MQ=60)
                             AACAATGATAGAAACCATTACTCATGGTGCAGAGTGGTTTATCGGGCTGTTCCAAAAGGGCGGAGAGGTGTTTACCGGGATGGTGACCGGCATT                                                                  >  SRR4302537.1417925/1‑94 (MQ=60)
                              ACAATGATAGAAACCATTACTCATGGTGCAGAGTGGTTTATCGGGCTGTTCCAAAAGGGCGGAGAGGTGTTTACCGGGATGGTGACCGGCATTT                                                                 >  SRR4302537.5794462/1‑94 (MQ=60)
                              ACAATGATAGAAACCATTACTCATGGTGCAGAGTGGTTTATCGGGCTGTTCCAAAAGGGCGGAGAGGTGTTTACCGGGATGGTGACCGGCATTC                                                                 >  SRR4302537.6175461/1‑94 (MQ=60)
                              ACAATGATAGAAACCATTACTCATGGTGCAGAGTGGTTTATCGGGCTGTTCCAAAAGGGCGGAGAGGTGTTTACCGGGATGGTGACCGGCATTC                                                                 >  SRR4302537.1582065/1‑94 (MQ=60)
                               CAATGATAGAAACCATTACTCATGGTGCAGAGTGGTTTATCGGGCTGTTCCAAAAGGGCGGAGAGGTGTTTACCGGGATGGTGACCGGCATTCT                                                                <  SRR4302537.307653/94‑1 (MQ=60)
                               CAATGATAGAAACCATTACTCATGGTGCAGAGTGGTTTATCGGGCTGTTCCAAAAGGGCGGAGAGGTGTTTACCGGGATGGTGACCGGCATTCT                                                                >  SRR4302537.1308913/1‑94 (MQ=60)
                               CAATGATAGAAACCATTACTCATGGGGCAGAGTGGTTTATCGGGCTGTTCCAAAAGGGCGGAGAGGCGTTTACCGGGATGGTGACCGGCATTCT                                                                >  SRR4302537.5843833/1‑94 (MQ=60)
                                AATGATAGAAACCATTACTCATGGTGCAGAGTGGTTTATCGGGCTGTTCCAAAAGGGCGGAGAGGTGTTTACCGGGATGGTGACCGGCATTCTT                                                               <  SRR4302537.3979990/94‑1 (MQ=60)
                                AATGATAGAAACCATTACTCATGGTGCAGAGTGGTTTATCGGGCTGTTCCAAAAGGGCGGAGAGGTGTTTACCGGGATGGTGACCGGCATTCTT                                                               <  SRR4302537.2188524/94‑1 (MQ=60)
                                 ATGATAGAAACCATTACTCATGGTGCAGAGTGGTTTATCGGGCTGTTCCAAAAGGGCGGAGAGGTGTTTACCGGGATGGTGACCGGCATTCTTC                                                              >  SRR4302537.2130917/1‑94 (MQ=60)
                                  AGATAGAAACCATTACTCATGGTGCAGAGTGGTTTATCGGGCTGTTCCAAAAGGGCGGAGAGGTGTTTACCGGGATGGTGACCGGCATTCTTCC                                                             <  SRR4302537.3437201/94‑1 (MQ=60)
                                   GATAGAAACCATTACTCATGGTGCAGAGTGGTTTATCGGGCTGTTCCAAAAGGGCGGAGAGGTGTTTACCGGGATGGTGACCGGCATTCTTCCG                                                            >  SRR4302537.3834963/1‑94 (MQ=60)
                                    ATAGAAACCATTACTCATGGTGCAGAGTGGTTTATCGGGCTGTTCCAAAAGGGCGGAGAGGTGTTTACCGGGATGGTGACCGGCATTCTTCCGC                                                           >  SRR4302537.3126478/1‑94 (MQ=60)
                                    ATAGAAACCATTACTCATGGTGCAGAGTGGTTTATCGGGCTGTTCCAAAAGGGCGGAGAGGTGTTTACCGGGATGGTGACCGGCATTCTTCCGC                                                           >  SRR4302537.2877218/1‑94 (MQ=60)
                                     TAGAAACCATTACTCATGGTGCAGAGTGGTTTATCGGGCTGTTCCAAAAGGGCGGAGAGGTGTTTACCGGGATGGTGACCGGCATTCTTCCGCT                                                          <  SRR4302537.644424/94‑1 (MQ=60)
                                      AGAAACCATTACTCATGGTGCAGAGTGGTTTATCGGGCTGTTCCAAAAGGGCGGAGAGGTGTTTACCGGGATGGTGACCGGCATTCTTCCGCTG                                                         <  SRR4302537.5610521/94‑1 (MQ=60)
                                        AAACCATTACTCATGGTGCAGAGTGGTTTATCGGGCTGTTCCAAAAGGGCGGAGAGGTGTTTACCGGGATGGTGACCGGCATTCTTCCGCTGTT                                                       <  SRR4302537.5313618/94‑1 (MQ=60)
                                         AACCATTACTCATGGTGCAGAGTGGTTTATCGGGCTGTTCCAAAAGGGCGGAGAGGTGTTTACCGGGATGGTGACCGGCATTCTTCCGCTGTTG                                                      >  SRR4302537.4168040/1‑94 (MQ=60)
                                         AACCATTACTCATGGTGCAGAGTGGTTTATCGGGCTGTTCCAAAAGGGCGGAGAGGTGTTTACCGGGATGGTGACCGGCATTCTTCCGCTGTTG                                                      <  SRR4302537.4621168/94‑1 (MQ=60)
                                         AACCATTACTCATGGTGCAGAGTGGTTTATCGGGCTGTTCCAAAAGGGCGGAGAGGTGTTTACCGGGATGGTGACCGGCATTCTTCCGCTGTTG                                                      >  SRR4302537.2547898/1‑94 (MQ=60)
                                         AACCATTACTCATGGTGCAGAGTGGTTTATCGGGCTGTTCCAAAAGGGCGGAGAGGTGTTTACCGGGATGGTGACCGGCATTCTTCCGCTGTTG                                                      >  SRR4302537.4902569/1‑94 (MQ=60)
                                          ACCATTACTCATGGTGCAGAGTGGTTTATCGGGCTGTTCCAAAAGGGCGGAGAGGTGTTTACCGGGATGGTGACCGGCATTCTTCCGCTGTTGA                                                     >  SRR4302537.2310052/1‑94 (MQ=60)
                                            CATTACTCATGGTGCAGAGTGGTTTATCGGGCTGTTCCAAAAGGGCGGAGAGGTGTTTACCGGGATGGTGACCGGCATTCTTCCGCTGTTGATT                                                   >  SRR4302537.499684/1‑94 (MQ=60)
                                            CATTACTCATGGTGCAGAGTGGTTTATCGGGCTGTTCCAAAAGGGCGGAGAGGTGTTTACCGGGATGGTGACCGGCATTCTTCCGCTGTTGATT                                                   >  SRR4302537.6048783/1‑94 (MQ=60)
                                              TTACTCATGGTGCAGAGTGGTTTATCGGGCTGTTCCAAAAGGGCGGAGAGGTGTTTACCGGGATGGTGACCGGCATTCTTCCGCTGTTGATTAG                                                 <  SRR4302537.3923682/94‑1 (MQ=60)
                                              TTACTCATGGTGCAGAGTGGTTTATCGGGCTGTTCCAAAAGGGCGGAGAGGTGTTTACCGGGATGGTGACCGGCATTCTTCCGCTGTTGATTAG                                                 <  SRR4302537.2353404/94‑1 (MQ=60)
                                              TTACTCATGGTGCAGAGTGGTTTATCGGGCTGTTCCAAAAGGGCGGAGAGGTGTTTACCGGGATGGTGACCGGCATTCTTCCGCTGTTGATTAG                                                 <  SRR4302537.3584217/94‑1 (MQ=60)
                                                 CTCATGGTGCAGAGTGGTTTATCGGGCTGTTCCAAAAGGGCGGAGAGGTGTTTACCGGGATGGTGACCGGCATTCTTCCGCTGTTGATTAGCCT                                              <  SRR4302537.2089389/94‑1 (MQ=60)
                                                     TGGTGCAGAGTGGTTTATCGGGCTGTTCCAAAAGGGCGGAGAGGTGTTTACCGGGATGGTGACCGGCATTCTTCCGCTGTTGATTAGCCTGCTG                                          <  SRR4302537.6021004/94‑1 (MQ=60)
                                                      GGTGCAGAGTGGTTTATCGGGCTGTTCCAAAAGGGCGGAGAGGTGTTTACCGGGATGGTGACCGGCATTCTTCCGCTGTTGATTAGCCTGCTGG                                         >  SRR4302537.4046004/1‑94 (MQ=60)
                                                      GGTGCAGAGTGGTTTATCGGGCTGTTCCAAAAGGGCGGAGAGGTGTTTACCGGGATGGTGACCGGCATTCTTCCGCTGTTGATTAGCCTGCTGG                                         <  SRR4302537.3777831/94‑1 (MQ=60)
                                                      GGTGCAGAGTGGTTTATCGGGCTGTTCCAAAAGGGCGGAGAGGTGTTTACCGGGATGGTGACCGGCATTCTTCCGCTGTTGATTAGCCTGCTGG                                         <  SRR4302537.1013949/94‑1 (MQ=60)
                                                       GTGCAGAGTGGTTTATCGGGCTGTTCCAAAAGGGCGGAGAGGTGTTTACCGGGATGGTGACCGGCATTCTTCCGCTGTTGATTAGCCTGCTGGT                                        >  SRR4302537.1344377/1‑94 (MQ=60)
                                                        TGCAGAGTGGTTTATCGGGCTGTTCCAAAAGGGCGGAGAGGTGTTTACCGGGATGGTGACCGGCATTCTTCCGCTGTTGATTAGCCTGCTGGTT                                       >  SRR4302537.4320138/1‑94 (MQ=60)
                                                        TGCAGAGTGGTTTATCGGGCTGTTCCAAAAGGGCGGAGAGGTGTTTACCGGGATGGTGACCGGCATTCTTCCGCTGTTGATTAGCCTGCTGGTT                                       <  SRR4302537.3266164/94‑1 (MQ=60)
                                                        TGCAGAGTGGTTTATCGGGCTGTTCCAAAAGGGCGGAGAGGTGTTTACCGGGATGGTGACCGGCATTCTTCCGCTGTTGATTAGCCTGCTGGTT                                       <  SRR4302537.2101818/94‑1 (MQ=60)
                                                          CAGAGTGGTTTATCGGGCTGTTCCAAAAGGGCGGAGAGGTGTTTACCGGGATGGTGACCGGCATTCTTCCGCTGTTGATTAGCCTGCTGGTTAT                                     <  SRR4302537.637315/94‑1 (MQ=60)
                                                          CAGAGTGGTTTATCGGGCTGTTCCAAAAGGGCGGAGAGGTGTTTACCGGGATGGTGACCGGCATTCTTCCGCTGTTGATTAGCCTGCTGGTTAT                                     >  SRR4302537.3666337/1‑94 (MQ=60)
                                                           AGAGTGGTTTATCGGGCTGTTCCAAAAGGGCGGAGAGGTGTTTACCGGGATGGTGACCGGCATTCTTCCGCTGTTGATTAGCCTGCTGGTTATC                                    >  SRR4302537.2888311/1‑94 (MQ=60)
                                                           AGAGTGGTTTATCGGGCTGTTCCAAAAGGGCGGAGAGGTGTTTACCGGGATGGTGACCGGCATTCTTCCGCTGTTGATTAGCCTGCTGGTTATC                                    >  SRR4302537.1300739/1‑94 (MQ=60)
                                                           AGAGTGGTTTATCGGGCTGTTCCAAAAGGGCGGAGAGGTGTTTACCGGGATGGTGACCGGCATTCTTCCGCTGTTGATTAGCCTGCTGGTTATC                                    >  SRR4302537.5346956/1‑94 (MQ=60)
                                                           AGAGTGGTTTATCGGGCTGTTCCAAAAGGGCGGAGAGGTGTTTACCGGGATGGTGACCGGCATTCTTCCGCTGTTGATTAGCCTGCTGGTTATC                                    >  SRR4302537.5561625/1‑94 (MQ=60)
                                                            GAGTGGTTTATCGGGCTGTTCCAAAAGGGCGGAGAGGTGTTTACCGGGATGGTGACCGGCATTCTTCCGCTGTTGATTAGCCTGCTGGTTATCA                                   <  SRR4302537.738543/94‑1 (MQ=60)
                                                            GAGTGGTTTATCGGGCTGTTCCAAAAGGGCGGAGAGGTGTTTACCGGGATGGTGACCGGCATTCTTCCGCTGTTGATTAGCCTGCTGGTTATCA                                   <  SRR4302537.5140393/94‑1 (MQ=60)
                                                            GAGTGGTTTATCGGGCTGTTCCAAAAGGGCGGAGAGGTGTTTACCGGGATGGTGACCGGCATTCTTCCGCTGTTGATTAGCCTGCTGGTTATCA                                   <  SRR4302537.3006724/94‑1 (MQ=60)
                                                            GAGTGGTTTATCGGGCTGTTCCAAAAGGGCGGAGAGGTGTTTACCGGGATGGTGACCGGCATTCTTCCGCTGTTGATTAGCCTGCTGGTTATCA                                   <  SRR4302537.2304814/94‑1 (MQ=60)
                                                            GAGTGGTTTATCGGGCTGTTCCAAAAGGGCGGAGAGGTGTTTACCGGGATGGTGACCGGCATTCTTCCGCTGTTGATTAGCCTGCTGGTTATCA                                   <  SRR4302537.1852993/94‑1 (MQ=60)
                                                            GAGTGGTTTATCGGGCTGTTCCAAAAGGGCGGAGAGGTGTTTACCGGGATGGTGACCGGCATTCTTCCGCTGTTGATTAGCCTGCTGGTTATCA                                   <  SRR4302537.1714703/94‑1 (MQ=60)
                                                             AGTGGTTTATCGGGCTGTTCCAAAAGGGCGGAGAGGGGTTTACCGGGATGGTGACCGGCATTCTTCCGCTGTTGATTAGCCTGCTGGTTATCAT                                  >  SRR4302537.3099864/1‑94 (MQ=60)
                                                               TGGTTTATCGGGCTGTTCCAAAAGGGCGGAGAGGTGTTTACCGGGATGGTGACCGGCATTCTTCCGCTGTTGATTAGCCTGCTGGTTATCATGA                                <  SRR4302537.2827928/94‑1 (MQ=60)
                                                                GGTTTATCGGGCTGTTCCAAAAGGGCGGAGAGGTGTTTACCGGGATGGTGACCGGCATTCTTCCGCTGTTGATTAGCCTGCTGGTTATCATGAA                               >  SRR4302537.6367704/1‑94 (MQ=60)
                                                                GGTTTATCGGGCTGTTCCAAAAGGGCGGAGAGGTGTTTACCGGGATGGTGACCGGCATTCTTCCGCTGTTGATTAGCCTGCTGGTTATCATGAA                               <  SRR4302537.4347084/94‑1 (MQ=60)
                                                                GGTTTATCGGGCTGTTCCAAAAGGGCGGAGAGGTGTTTACCGGGATGGTGACCGGCATTCTTCCGCTGTTGATTAGCCTGCTGGTTATCATGAA                               <  SRR4302537.2754525/94‑1 (MQ=60)
                                                                GGTTTATCGGGCTGTTCCAAAAGGGCGGAGAGGTGTTTACCGGGATGGTGACCGGCATTCTTCCGCTGTTGATTAGCCTGCTGGTTATCATGAA                               >  SRR4302537.3312858/1‑94 (MQ=60)
                                                                 GTTTATCGGGCTGTTCCAAAAGGGCGGAGAGGTGTTTACCGGGATGGTGACCGGCATTCTTCCGCTGTTGATTAGCCTGCTGGTTATCATGAAC                              <  SRR4302537.3583405/94‑1 (MQ=60)
                                                                  TTTATCGGGCTGTTCCAAAAGGGCGGAGAGGTGTTTACCGGGATGGTGACCGGCATTCTTCCGCTGTTGATTAGCCTGCTGGTTATCATGAACG                             >  SRR4302537.5928042/1‑94 (MQ=60)
                                                                  TTTATCGGGCTGTTCCAAAAGGGCGGAGAGGTGTTTACCGGGATGGTGACCGGCATTCTTCCGCTGTTGATTAGCCTGCTGGTTATCATGAACG                             <  SRR4302537.997907/94‑1 (MQ=60)
                                                                    TATCGGGCTGTTCCAAAAGGGCGGAGAGGTGTTTACCGGGATGGTGACCGGCATTCTTCCGCTGTTGATTAGCCTGCTGGTTATCATGAACGCA                           >  SRR4302537.1065070/1‑94 (MQ=60)
                                                                       CGCGCTGTTACAAAAGGGCGGAGAGGTGTTTACCGGGATGGTGACCGGCATTCTTCCGCTGTTGATTAGCCTGCTGGTTATCATGAACGCACTG                        <  SRR4302537.5095487/94‑1 (MQ=60)
                                                                        GGGCTGTTCCAAAAGGGCGGAGAGGTGTTTACCGGGATGGTGACCGGCATTCTTCCGCTGTTGATTAGCCTGCTGGTTATCATGAACGCACTGA                       >  SRR4302537.264910/1‑94 (MQ=60)
                                                                         GGCTGTTCCAAAAGGGCGGAGAGGTGTTTACCGGGATGGTGACCGGCATTCTTCCGCTGTTGATTAGCCTGCTGGTTATCATGAACGCACTGAT                      <  SRR4302537.3167233/94‑1 (MQ=60)
                                                                           CTGTTCCAAAAGGGCGGAGAGGTGTTTACCGGGATGGTGACCGGCATTCTTCCGCTGTTGATTAGCCTGCTGGTTATCATGAACGCACTGATTA                    >  SRR4302537.5253454/1‑94 (MQ=60)
                                                                           CTGTTCCAAAAGGGCGGAGAGGTGTTTACCGGGATGGTGACCGGCATTCTTCCGCTGTTGATTAGCCTGCTGGTTATCATGAACGCACTGATTA                    >  SRR4302537.4330987/1‑94 (MQ=60)
                                                                           CTGTTCCAAAAGGGCGGAGAGGTGTTTACCGGGATGGTGACCGGCATTCTTCCGCTGTTGATTAGCCTGCTGGTTATCATGAACGCACTGATTA                    >  SRR4302537.282182/1‑94 (MQ=60)
                                                                            GGTTCCAAAAGGGCGTAGAGGTGTTTACCGGGATGGTGATCTGCAGTCTTCCGCTGTTGATTAGCCTGCTGTTTATCATGAACGCACTGATTAA                   <  SRR4302537.3064487/94‑1 (MQ=60)
                                                                             GTTCCAAAAGGGCGGAGAGGTGTTTACCGGGATGGTGACCGGCATTCTTCCGCTGTTGATTAGCCTGCTGGTTATCATGAACGCACTGATTAAT                  >  SRR4302537.6332438/1‑94 (MQ=60)
                                                                             GTTCCAAAAGGGCGGAGAGGTGTTTACCGGGATGGTGACCGGCATTCTTCCGCTGTTGATTAGCCTGCTGGTTATCATGAACGCACTGATTAAT                  <  SRR4302537.2239866/94‑1 (MQ=60)
                                                                             GTTCCAAAAGGGCGGAGAGGTGTTTACCGGGATGGTGACCGGCATTCTTCCGCTGTTGATTAGCCTGCTGGTTATCATGAACGCACTGATTAAT                  >  SRR4302537.839587/1‑94 (MQ=60)
                                                                              TTCCAAAAGGGCGGAGAGGTGTTTACCGGGATGGTGACCGGCATTCTTCCGCTGTTGATTAGCCTGCTGGTTATCATGAACGCACTGATTAATT                 <  SRR4302537.4419359/94‑1 (MQ=60)
                                                                               TCCAAAAGGGCGGAGAGGTGTTTACCGGGATGGTGACCGGCATTCTTCCGCTGTTGATTAGCCTGCTGGTTATCATGAACGCACTGATTAATTT                <  SRR4302537.4986966/94‑1 (MQ=60)
                                                                               TCCAAAAGGGCGGAGAGGTGTTTACCGGGATGGTGACCGGCATTCTTCCGCTGTTGATTAGCCTGCTGGTTATCATGAACGCACTGATTAATTT                <  SRR4302537.2724166/94‑1 (MQ=60)
                                                                               TCCAAAAGGGCGGAGAGGTGTTTACCGGGATGGTGACCGGCATTCTTCCGCTGTTGATTAGCCTGCTGGTTATCATGAACGCACTGATTAATTT                <  SRR4302537.620462/94‑1 (MQ=60)
                                                                                CCAAAAGGGCGGAGAGGTGTTTACCGGGATGGTGACCGGCATTCTTCCGCTGTTGATTAGCCTGCTGGTTATCATGAACGCACTGATTAATTTT               >  SRR4302537.4308533/1‑94 (MQ=60)
                                                                                CCAAAAGGGCGGAGAGGTGTTTACCGGGATGGTGACCGGCATTCTTCCGCTGTTGATTAGCCTGCTGGTTATCATGAACGCACTGAGTAATTTT               >  SRR4302537.5292337/1‑94 (MQ=60)
                                                                                  AAAAGGGCGGAGAGGTGTTTACCGGGATGGTGACCGGCATTCTTCCGCTGTTGATTAGCCTGCTGGTTATCATGAACGCACTGATTAATTTTAT             <  SRR4302537.2250241/94‑1 (MQ=60)
                                                                                    AAGGGCGGAGAGGTGTTTACCGGGATGGTGACCGGCATTCTTCCGCTGTTGATTAGCCTGCTGGTTATCATGAACGCACTGATTAATTTTATCG           <  SRR4302537.618200/94‑1 (MQ=60)
                                                                                    AAGGGCGGAGAGGTGTTTACCGGGATGGTGACCGGCATTCTTCCGCTGTTGATTAGCCTGCTGGTTATCATGAACGCACTGATTAATTTTATCG           <  SRR4302537.5015794/94‑1 (MQ=60)
                                                                                      GGGCGGAGAGGTGTTTACCGGGATGGTGACCGGCATTCTTCCGCTGTTGATTAGCCTGCTGGTTATCATGAACGCACTGATTAATTTTATCGGT         <  SRR4302537.3836328/94‑1 (MQ=60)
                                                                                       GGCGGAGAGGTGTTTACCGGGATGGTGACCGGCATTCTTCCGCTGTTGATTAGCCTGCTGGTTATCATGAACGCACTGATTAATTTTATCGGTC        <  SRR4302537.2901122/94‑1 (MQ=60)
                                                                                        GCGGAGAGGTGTTTACCGGGATGGTGACCGGCATTCTTCCGCTGTTGATTAGCCTGCTGGTTATCATGAACGCACTGATTAATTTTATCGGTCA       <  SRR4302537.6125404/94‑1 (MQ=60)
                                                                                        GCGGAGAGGTGTTTACCGGGATGGTGACCGGCATTCTTCCGCTGTTGATTAGCCTGCTGGTTATCATGAACGCACTGATTAATTTTATCGGTCA       >  SRR4302537.2026935/1‑94 (MQ=60)
                                                                                        GCGGAGAGGTGTTTACCGGGATGGTGACCGGCATTCTTCCGCTGTTGATTAGCCTGCTGGTTATCATGAACGCACTGATTAATTTTATCGGTCA       <  SRR4302537.4715694/94‑1 (MQ=60)
                                                                                        GCGGAGAGGTGTTTACCGGGATGGTGACCGGCATTCTTCCGCTGTTGATTAGCCTGCTGGTTATCATGAACGCACTGATTAATTTTATCGGTCA       <  SRR4302537.738447/94‑1 (MQ=60)
                                                                                         CGGAGAGGTGTTTACCGGGATGGTGACCGGCATTCTTCCGCTGTTGATTAGCCTGCTGGTTATCATGAACGCACTGATTAATTTTATCGGTCAG      <  SRR4302537.1917358/94‑1 (MQ=60)
                                                                                         CGGAGAGGTGTTTACCGGGATGGTGACCGGCATTCTTCCGCTGTTGATTAGCCTGCTGGTTATCATGAACGCACTGATTAATTTTATCGGTCAG      <  SRR4302537.6471418/94‑1 (MQ=60)
                                                                                            AGAGGTGTTTACCGGGATGGTGACCGGCATTCTTCCGCTGTTGATTAGCCTGCTGGTTATCATGAACGCACTGATTAATTTTATCGGTCAGCAT   >  SRR4302537.123512/1‑94 (MQ=60)
                                                                                            AGAGGTGTTTACCGGGATGGTGACCGGCATTCTTCCGCTGTTGATTAGCCTGCTGGTTATCATGAACGCACTGATTAATTTTATCGGTCAGCAT   <  SRR4302537.2568068/94‑1 (MQ=60)
                                                                                             GAGGTGTTTACCGGGATGGTGACCGGCATTCTTCCGCTGTTGATTAGCCTGCTGGTTATCATGAACGCACTGATTAATTTTATCGGTCAGCATC  <  SRR4302537.655251/94‑1 (MQ=60)
                                                                                             GAGGTGTTTACCGGGATGGTGACCGGCATTCTTCCGCTGTTGATTAGCCTGCTGGTTATCATGAACGCACTGATTAATTTTATCGGTCAGCATC  >  SRR4302537.4144582/1‑94 (MQ=60)
                                                                                             |                                                                                             
GTAATAAAACAATAAATCCTGAAGGAGAGAACAATGATAGAAACCATTACTCATGGTGCAGAGTGGTTTATCGGGCTGTTCCAAAAGGGCGGAAAGGTGTTTACCGGGATGGTGACCGGCATTCTTCCGCTGTTGATTAGCCTGCTGGTTATCATGAACGCACTGATTAATTTTATCGGTCAGCATC  >  NC_000913/2823822‑2824008

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑