Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I2 R1 882 254.4 12957588 98.0% 12698436 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,824,797 T→C F128S (TTT→TCT)  srlE → PTS system glucitol/sorbitol‑specific enzyme IIBC component

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,824,7970TC100.0% 800.1 / NA 228F128S (TTT→TCT) srlEPTS system glucitol/sorbitol‑specific enzyme IIBC component
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (139/89);  total (139/89)

Only 100 of 235 total aligned reads displayed.

CGCTGGCGCAGTACATTGTGGAAGATATTTATGTCTCTGGCGTAAAAGAAGAAAACATCACTGTAGTAGGTGATGCGACACCACAACCCTTTTCCGTGGGCCGTGACTATGACACCAGTAAGAAAATCACCGAACAAAGCGATGGTTTACTGGCGAAGGTGGGAATGGGCATGGGGTCCA  >  NC_000913/2824707‑2824886
                                                                                          |                                                                                         
cgCTGGCGCAGTACATTGTGGAAGATATTTATGTCTCTGGCGTAAAAGAAGAAAACATCACTGTAGTAGGTGATGCGACACCACAACCCtctt                                                                                         <  1:1275186/93‑1 (MQ=255)
 gCTGGCGCAGTACATTGTGGAAGATATTTATGTCTCTGGCGTAAAAGAAGAAAACATCACTGTAGTAGGTGATGCGACACCACAACCCTCTTc                                                                                        <  2:768695/93‑1 (MQ=255)
 gCTGGCGCAGTACATTGTGGAAGATATTTATGTCTCTGGCGTAAAAGAAGAAAACATCACTGTAGTAGGTGATGCGACACCACAACCCTCTTc                                                                                        >  2:434462/1‑93 (MQ=255)
 gCTGGCGCAGTACATTGTGGAAGATATTTATGTCTCTGGCGTAAAAGAAGAAAACATCACTGTAGTAGGTGATGCGACACCACAACCCTCTTc                                                                                        <  1:1572966/93‑1 (MQ=255)
 gCTGGCGCAGTACATTGTGGAAGATATTTATGTCTCTGGCGTAAAAGAAGAAAACATCACTGTAGTAGGTGATGCGACACCACAACCCTCTTc                                                                                        <  1:4234145/93‑1 (MQ=255)
  cTGGCGCAGTACATTGTGGAAGATATTTATGTCTCTGGCGTAAAAGAAGAAAACATCACTGTAGTAGGTGATGCGACACCACAACCCTCTTcc                                                                                       >  1:4958092/1‑93 (MQ=255)
  cTGGCGCAGTACATTGTGGAAGATATTTATGTCTCTGGCGTAAAAGAAGAAAACATCACTGTAGTAGGTGATGCGACACCACAACCCTCTTcc                                                                                       >  1:3516157/1‑93 (MQ=255)
   tGGCGCAGTACATTGTGGAAGATATTTATGTCTCTGGCGTAAAAGAAGAAAACATCACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCg                                                                                      >  1:141080/1‑93 (MQ=255)
   tGGCGCAGTACATTGTGGAAGATATTTATGTCTCTGGCGTAAAAGAAGAAAACATCACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCg                                                                                      >  2:1779401/1‑93 (MQ=255)
   tGGCGCAGTACATTGTGGAAGATATTTATGTCTCTGGCGTAAAAGAAGAAAACATCACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCg                                                                                      >  2:1458648/1‑93 (MQ=255)
     gcgcAGTACATTGTGGAAGATATTTATGTCTCTGGCGTATAAGAAGAAAACATCACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTg                                                                                    >  1:4669302/1‑93 (MQ=255)
     gcgcAGTACATTGTGGAAGATATTTATGTCTCTGGCGTAAAAGAAGAAAACATCACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTg                                                                                    <  2:3246394/93‑1 (MQ=255)
     gcgcAGTACATTGTGGAAGATATTTATGTCTCTGGCGTAAAAGAAGAAAACATCACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTg                                                                                    >  1:1591181/1‑93 (MQ=255)
      cgcAGTACATTGTGGAAGATATTTATGTCTCTGGCGTAAAAGAAGAAAACATCACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTgg                                                                                   <  1:1773130/93‑1 (MQ=255)
       gcAGTACATTGTGGAAGATATTTATGTCTCTGGCGTAAAAGAAGAAAACATCACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTggg                                                                                  >  1:6359896/1‑93 (MQ=255)
       gcAGTACATTGTGGAAGATATTTATGTCTCTGGCGTAAAAGAAGAAAACATCACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTggg                                                                                  <  1:4090253/93‑1 (MQ=255)
        cAGTACATTGTGGAAGATATTTATGTCTCTGGCGTAAAAGAAGAAAACATCACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGc                                                                                 >  1:5336069/1‑93 (MQ=255)
         aGTACATTGTGGAAGATATTTATGTCTCTGGCGTAAAAGAAGAAAACATCACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGcc                                                                                >  1:1193671/1‑93 (MQ=255)
           tACATTGTGGAAGATATTTATGTCTCTGGCGTAAAAGAAGAAAACATCACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGCCGt                                                                              <  2:4382265/93‑1 (MQ=255)
            aCATTGTGGAAGATATTTATGTCTCTGGCGTAAAAGAAGAAAACATCACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTg                                                                             >  2:4964667/1‑93 (MQ=255)
             cATTGTGGAAGATATTTATGTCTCTGGCGTAAAAGAAGAAAACATCACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGa                                                                            >  1:3733761/1‑93 (MQ=255)
              aTTGTGGAAGATATTTATGTCTCTGGCGTAAAAGAAGAAAACATCACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGAc                                                                           >  1:4314039/1‑93 (MQ=255)
               ttGTGGAAGATATTTATGTCTCTGGCGTAAAAGAAGAAAACATCACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGACt                                                                          >  1:2815299/1‑93 (MQ=255)
                 gtgGAAGATATTTATGTCTCTGGCGTAAAAGAAGAAAACATCACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTAt                                                                        >  2:59877/1‑93 (MQ=255)
                  tgGAAGATATTTATGTCTCTGGCGTAAAAGAAGAAAACATCACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTATg                                                                       >  2:2419343/1‑93 (MQ=255)
                   ggAAGATATTTATGTCTCTGGCGTAAAAGAAGAAAACATCACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTATGa                                                                      >  1:5870665/1‑93 (MQ=255)
                     aaGATATTTATGTCTCTGGCGTAAAAGAAGAAAACATCACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTATGaca                                                                    >  2:1189380/1‑93 (MQ=255)
                       gATATTTATGTCTCTGGCGTAAAAGAAGACAACATCACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTATGACAcc                                                                  >  2:5305707/1‑93 (MQ=255)
                       gATATTTATGTCTCTGGCGTAAAAGAAGAAAACATCACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTATGACAcc                                                                  <  1:2044554/93‑1 (MQ=255)
                       gATATTTATGTCTCTGGCGTAAAAGAAGAAAACATCACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTATGACAcc                                                                  >  1:4570399/1‑93 (MQ=255)
                        atatTTATGTCTCTGGCGTAAAAGAAGAAAACATCACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTATGACACCa                                                                 >  1:3271671/1‑93 (MQ=255)
                        atatTTATGTCTCTGGCGTAAAAGAAGAAAACATCACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTATGACACCa                                                                 >  1:6306345/1‑93 (MQ=255)
                         tatTTATGTCTCTGGCGTAAAAGAAGAAAACATCACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTATGACACCAg                                                                >  2:4586736/1‑93 (MQ=255)
                         tatTTATGTCTCTGGCGTAAAAGAAGAAAACATCACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTATGACACCAg                                                                <  1:5513787/93‑1 (MQ=255)
                          atTTATGTCTCTGGCGTAAAAGAAGAAAACATCACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTATGACACCAGt                                                               >  1:1033495/1‑93 (MQ=255)
                           tttATGTCTCTGGCGTAAAAGAAGAAAACATCACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTATGACACCAGTa                                                              <  1:2757041/93‑1 (MQ=255)
                           tttATGTCTCTGGCGTAAAAGAAGAAAACATCACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTATGACACCAGTa                                                              >  1:5062887/1‑93 (MQ=255)
                           tttATGTCTCTGGCGTAAAAGAAGAAAACATCACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTATGACACCAGTa                                                              <  2:2318784/93‑1 (MQ=255)
                             tATGTCTCTGGCGTAAAAGAAGAAAACATCACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAg                                                            >  1:4733831/1‑93 (MQ=255)
                              aTGTCTCTGGCGTAAAAGAAGAAAACATCACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAGa                                                           <  2:2133270/93‑1 (MQ=255)
                                 tctcTGGCGTAAAAGAAGAAAACATCACTGTAGTAGGTGATGCGACACCACAACCCTCTTGCGTGGGCCGTGACTATGACACCAGTAAGaaaa                                                        <  1:4868464/93‑1 (MQ=255)
                                  ctctGGCGTAAAAGAAGAAAACATCACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAGAAAAt                                                       >  2:5650802/1‑93 (MQ=255)
                                   tctGGCGTAAAAGAAGAAAACATCACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAGAAAATc                                                      >  2:5027698/1‑93 (MQ=255)
                                   tctGGCGTAAAAGAAGAAAACATCACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAGAAAATc                                                      >  1:5187228/1‑93 (MQ=255)
                                    ctGGCGTAAAAGAAGAAAACATCACTGTAGTAGGTGATGCGACTCCACAACCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAGAAAATCa                                                     <  2:249683/93‑1 (MQ=255)
                                    ctGGCGTAAAAGAAGAAAACATCACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAGAAAATCa                                                     <  1:2481929/93‑1 (MQ=255)
                                           aaaaGAAGAAAACATCACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAGAAAATCACCGAACa                                              <  1:2211638/93‑1 (MQ=255)
                                           aaaaGAAGAAAACATCACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAGAAAATCACCGAACa                                              >  2:5907876/1‑93 (MQ=255)
                                            aaaGAAGAAAACATCACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAGAAAATCACCGAACaa                                             <  1:5489985/93‑1 (MQ=255)
                                            aaaGAAGAAAACATCACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAGAAAATCACCGAACaa                                             >  1:596736/1‑93 (MQ=255)
                                             aagaagAAAACATCACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAGAAAATCACCGAACaaa                                            <  1:2084996/93‑1 (MQ=255)
                                             aagaagAAAACATCACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAGAAAATCACCGAACaaa                                            <  1:926991/93‑1 (MQ=255)
                                               gaagaaAACATCACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAGAAAATCACCGAACAAAGc                                          >  1:3378279/1‑93 (MQ=255)
                                               gaagaaAACATCACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAGAAAATCACCGAACAAAGc                                          >  2:2780483/1‑93 (MQ=255)
                                               gaagaaAACATCACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAGAAAATCACCGAACAAAGc                                          <  1:2028916/93‑1 (MQ=255)
                                               gaagaaAACATCACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAGAAAATCACCGAACAAAGc                                          >  2:2199631/1‑93 (MQ=255)
                                                 agaaAACATCACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAGAAAATCACCGAACAAAGCGa                                        >  2:3113718/1‑93 (MQ=255)
                                                 agaaAACATCACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAGAAAATCACCGAACAAAGCGa                                        <  2:5569311/93‑1 (MQ=255)
                                                  gaaAACATCACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAGAAAATCACCGAACAAAGCGAt                                       <  1:4698895/93‑1 (MQ=255)
                                                  gaaAACATCACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAGAAAATCACCGAACAAAGCGAt                                       <  2:1104134/93‑1 (MQ=255)
                                                  gaaAACATCACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAGAAAATCACCGAACAAAGCGAt                                       >  1:3297941/1‑93 (MQ=255)
                                                    aaaCATCACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAGAAAATCACCGAACAAAGCGATgg                                     >  2:5516703/1‑93 (MQ=255)
                                                    aaaCATCACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAGAAAATCACCGAACAAAGCGATgg                                     >  1:4017239/1‑93 (MQ=255)
                                                     aaCATCACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAGAAAATCACCGAACAAAGCGATGGt                                    >  2:3859798/1‑93 (MQ=255)
                                                        aTCACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAGAAAATCACCGAACAAAGCGATGGTTTa                                 >  2:4881975/1‑93 (MQ=255)
                                                        aTCACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAGAAAATCACCGAACAAAGCGATGGTTTa                                 >  1:1010105/1‑93 (MQ=255)
                                                        aTCACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAGAAAATCACCGAACAAAGCGATGGTTTa                                 >  1:5624653/1‑93 (MQ=255)
                                                        aTCACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAGAAAATCACCGAACAAAGCGATGGTTTa                                 <  2:4161739/93‑1 (MQ=255)
                                                         tCACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAGAAAATCACCGAACAAAGCGATGGTTTAc                                <  2:1818004/93‑1 (MQ=255)
                                                          cACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAGAAAATCACCGAACAAAGCGATGGTTTACt                               <  1:2552157/93‑1 (MQ=255)
                                                           aCTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTATGCCACCAGTAAGAAAATCACCGAACAAACCGATGGTTTACTg                              >  2:4799273/1‑93 (MQ=255)
                                                            cTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAGAAAATCACCGAACAAAGCGATGGTTTACTgg                             <  2:2807514/93‑1 (MQ=255)
                                                              gtagtaGGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAGAAAATCACCGAACAAAGCGATGGTTTACTGGCg                           >  2:5552637/1‑93 (MQ=255)
                                                               tagtagGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAGAAAATCACCGAACAAAGCGATGGTTTACTGGCGa                          <  2:15636/93‑1 (MQ=255)
                                                                agtagGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAGAAAATCACCGAACAAAGCGATGGTTTACTGGCGaa                         <  1:1319268/93‑1 (MQ=255)
                                                                  tagGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAGAAAATCACCGAACAAAGCGATGGTTTACTGGCGAAgg                       >  2:6336148/1‑93 (MQ=255)
                                                                   agGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAGAAAATCACCGAACAAAGCGATGGTTTACTGGCGAAGGt                      <  2:1534602/93‑1 (MQ=255)
                                                                    ggTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAGAAAATCACCGAACAAAGCGATGGTTTACTGGCGAAGGTg                     <  2:1022316/93‑1 (MQ=255)
                                                                     gTGATGCGACACCACAGCCCTCTTCCGTGAGCCGTCACTATGACACCAGTAAGAAAATCTCCGAACAAAGCGATGGTTTACTGGCGAAGGTgg                    <  1:3667910/93‑1 (MQ=255)
                                                                     gTGATGCGACACCACAACCCTCTTCCGTGGGCCGTTACTATGACACCAGTAAGAAAATCACCGAACAAAGCGATGGTTTACTGGCGAAGGTgg                    <  2:3818170/93‑1 (MQ=255)
                                                                     gTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAGAAAATCACCGAACAAAGCGATGGTTTACTGGCGAAGGTgg                    <  2:2865852/93‑1 (MQ=255)
                                                                     gTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAGAAAATCACCGAACAAAGCGATGGTTTACTGGCGAAGGTgg                    <  1:451019/93‑1 (MQ=255)
                                                                       gATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAGAAAATCACCGAACAAAGCGATGGTTTACTGGCGAAGGTGGGa                  >  1:5683556/1‑93 (MQ=255)
                                                                        aTGCGACACCACAACCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAGAAAATCACCGAACAAAGCGATGGTTTACTGGCGAAGGTGGGaa                 <  1:2460158/93‑1 (MQ=255)
                                                                         tGCGACACCACAACCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAGAAAATCACCGAACAAAGCGATGGTTTACTGGCGAAGGTGGGAAt                <  2:5384496/93‑1 (MQ=255)
                                                                         tGCGACACCACAACCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAGAAAATCACCGAACAAAGCGATGGTTTACTGGCGAAGGTGGGAAt                >  2:1958619/1‑93 (MQ=255)
                                                                           cGACACCACAACCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAGAAAATCACCGAACAAAGCGATGGTTTACTGGCGAAGGTGGGAATgg              <  2:1217148/93‑1 (MQ=255)
                                                                            gACACCACAACCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAGAAAATCACCGAACAAAGCGATGGTTTACTGGCGAAGGTGGGAATggg             >  2:3686232/1‑93 (MQ=255)
                                                                            gACACCACAACCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAGAAAATCACCGAACAAAGCGATGGTTTACTGGCGAAGGTGGGAATggg             <  2:2461207/93‑1 (MQ=255)
                                                                             acacCACAACCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAGAAAATCACCGAACAAAGCGATGGTTTACTGGCGAAGGTGGGAATGGGc            >  2:3732073/1‑93 (MQ=255)
                                                                             acacCACAACCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAGAAAATCACCGAACAAAGCGATGGTTTACTGGCGAAGGTGGGAATGGGc            <  1:4171396/93‑1 (MQ=255)
                                                                               accacAACCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAGAAAATCACCGAACAAAGCGATGGTTTACTGGCGAAGGTGGGAATGGGCAt          >  2:4615878/1‑93 (MQ=255)
                                                                                ccacAACCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAGAAAATCACCGAACAAAGCGATGGTTTACTGGCGAAGGTGGGAATGGGCATg         <  1:2887569/93‑1 (MQ=255)
                                                                                 cacaACCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAGAAAATCACCGAACAAAGCGATGGTTTACTGGCGAAGGTGGGAATGGGCATgg        >  1:861187/1‑93 (MQ=255)
                                                                                 cacaACCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAGAAAATCACCGAACAAAGCGATGGATTACTGGCGAAGGGGGGAATGGGCATgg        >  1:5762718/1‑93 (MQ=255)
                                                                                  acaACCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAGAAAATCACCGAACAAAGCGATGGTTTACTGGCGAAGGTGGGAATGGGCATggg       <  1:3037874/93‑1 (MQ=255)
                                                                                  acaACCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAGAAAATCACCGAACAAAGCGATGGTTTACTGGCGAAGGTGGGAATGGGCATggg       >  2:3501095/1‑93 (MQ=255)
                                                                                   caACCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAGAAAATCACCGAACAAAGCGATGGTTTACTGGCGAAGGTGGGAATGGGCATgggg      >  1:5086467/1‑93 (MQ=255)
                                                                                    aaCCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAGAAAATCACCGAACAAAGCGATGGTTTACTGGCGAAGGTGGGAATGGGCATGGGGt     >  2:605224/1‑93 (MQ=255)
                                                                                      cccTCTGCCGTGCGCCGTGACTATGACACCAGTAAGAAACTCACCGAACAACGTGTTGTTTTGCTGACAAAGGTGGGAATGGGCATGGGGTcc   <  2:3045052/93‑1 (MQ=255)
                                                                                          |                                                                                         
CGCTGGCGCAGTACATTGTGGAAGATATTTATGTCTCTGGCGTAAAAGAAGAAAACATCACTGTAGTAGGTGATGCGACACCACAACCCTTTTCCGTGGGCCGTGACTATGACACCAGTAAGAAAATCACCGAACAAAGCGATGGTTTACTGGCGAAGGTGGGAATGGGCATGGGGTCCA  >  NC_000913/2824707‑2824886

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 23 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
GTCCGCTGGCGCAGTACATTGTGGAAGATATTTATGTCTCTGGCGTAAAAGAAGAAAACATCACTGTAGTAGGTGATGCGACACCACAACCCTTTTCCGTGGGCCGTGACTATGACACCAGTAAGAAAATCACCGAACAAAGCGATGGTTTACTGGCGAAGGTGGGAATGGGCATGGGGTCCACC  >  NC_000913/2824704‑2824888
                                                                                             |                                                                                           
GTCCGCTGGCGCAGTACATTGTGGAAGATATTTATGTCTCTGGCGTAAAAGAAGAAAACATCACTGTAGTAGGTGATGCGACACCACAACCCTC                                                                                             >  SRR4302537.6005447/1‑94 (MQ=60)
   CGCTGGCGCAGTACATTGTGGAAGATATTTATGTCTCTGGCGTAAAAGAAGAAAACATCACTGTAGTAGGTGATGCGACACCACAACCCTCTTC                                                                                          <  SRR4302537.1284055/94‑1 (MQ=60)
    GCTGGCGCAGTACATTGTGGAAGATATTTATGTCTCTGGCGTAAAAGAAGAAAACATCACTGTAGTAGGTGATGCGACACCACAACCCTCTTCC                                                                                         >  SRR4302537.4992273/1‑94 (MQ=60)
    GCTGGCGCAGTACATTGTGGAAGATATTTATGTCTCTGGCGTAAAAGAAGAAAACATCACTGTAGTAGGTGATGCGACACCACAACCCTCTTCC                                                                                         >  SRR4302537.3541033/1‑94 (MQ=60)
    GCTGGCGCAGTACATTGTGGAAGATATTTATGTCTCTGGCGTAAAAGAAGAAAACATCACTGTAGTAGGTGATGCGACACCACAACCCTCTTCC                                                                                         <  SRR4302537.4263610/94‑1 (MQ=60)
    GCTGGCGCAGTACATTGTGGAAGATATTTATGTCTCTGGCGTAAAAGAAGAAAACATCACTGTAGTAGGTGATGCGACACCACAACCCTCTTCC                                                                                         <  SRR4302537.1583940/94‑1 (MQ=60)
     CTGGCGCAGTACATTGTGGAAGATATTTATGTCTCTGGCGTAAAAGAAGAAAACATCACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCG                                                                                        >  SRR4302537.4059128/1‑94 (MQ=60)
     CTGGCGCAGTACATTGTGGAAGATATTTATGTCTCTGGCGTAAAAGAAGAAAACATCACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCG                                                                                        >  SRR4302537.142083/1‑94 (MQ=60)
       GGCGCAGTACATTGTGGAAGATATTTATGTCTCTGGCGTATAAGAAGAAAACATCACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTG                                                                                      >  SRR4302537.4701606/1‑94 (MQ=60)
       GGCGCAGTACATTGTGGAAGATATTTATGTCTCTGGCGTAAAAGAAGAAAACATCACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTG                                                                                      >  SRR4302537.1602304/1‑94 (MQ=60)
         CGCAGTACATTGTGGAAGATATTTATGTCTCTGGCGTAAAAGAAGAAAACATCACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGG                                                                                    <  SRR4302537.1785481/94‑1 (MQ=60)
         CGCAGTACATTGTGGAAGATATTTATGTCTCTGGCGTAAAAGAAGAAAACATCACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGG                                                                                    >  SRR4302537.6403289/1‑94 (MQ=60)
         CGCAGTACATTGTGGAAGATATTTATGTCTCTGGCGTAAAAGAAGAAAACATCACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGG                                                                                    <  SRR4302537.5531029/94‑1 (MQ=60)
          GCAGTACATTGTGGAAGATATTTATGTCTCTGGCGTAAAAGAAGAAAACATCACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGC                                                                                   <  SRR4302537.4118821/94‑1 (MQ=60)
          GCAGTACATTGTGGAAGATATTTATGTCTCTGGCGTAAAAGAAGAAAACATCACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGC                                                                                   >  SRR4302537.5372655/1‑94 (MQ=60)
           CAGTACATTGTGGAAGATATTTATGTCTCTGGCGTAAAAGAAGAAAACATCACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGCC                                                                                  >  SRR4302537.1201959/1‑94 (MQ=60)
           CAGTACATTGTGGAAGATATTTATGTCTCTGGCGTAAAAGAAGAAAACATCACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGCC                                                                                  >  SRR4302537.3352225/1‑94 (MQ=60)
            AGTACATTGTGGAAGATATTTATGTCTCTGGCGTAAAAGAAGAAAACATCACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGCCG                                                                                 >  SRR4302537.1489800/1‑94 (MQ=60)
            AGTACATTGTGGAAGATATTTATGTCTCTGGCGTAAAAGAAGAAAACATCACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGCCG                                                                                 >  SRR4302537.6354129/1‑94 (MQ=60)
             GTACATTGTGGAAGATATTTATGTCTCTGGCGTAAAAGAAGAAAACATCACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGCCGT                                                                                >  SRR4302537.4012525/1‑94 (MQ=60)
             GTACATTGTGGAAGATATTTATGTCTCTGGCGTAAAAGAAGAAAACATCACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGCCGG                                                                                >  SRR4302537.4261624/1‑94 (MQ=60)
              TACATTGTGGAAGATATTTATGTCTCTGGCGTAAAAGAAGAAAACATCACTGTAGTAGGTGATGCGACCCCACAACCCTCTTCCGTGGGCCGTG                                                                               <  SRR4302537.5475923/94‑1 (MQ=60)
               ACATTGTGGAAGATATTTATGTCTCTGGCGTAAAAGAAGAAAACATCACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGA                                                                              >  SRR4302537.4631559/1‑94 (MQ=60)
               ACATTGTGGAAGATATTTATGTCTCTGGCGTAAAAGAAGAAAACATCACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGA                                                                              >  SRR4302537.3759987/1‑94 (MQ=60)
                CATTGTGGAAGATATTTATGTCTCTGGCGTAAAAGAAGAAAACATCACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGAC                                                                             >  SRR4302537.4344026/1‑94 (MQ=60)
                CATTGTGGAAGATATTTATGTCTCTGGCGTAAAAGAAGAAAACATCACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGAC                                                                             >  SRR4302537.2974168/1‑94 (MQ=60)
                CATTGTGGAAGATATTTATGTCTCTGGCGTAAAAGAAGAAAACATCACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGAC                                                                             >  SRR4302537.2481148/1‑94 (MQ=60)
                 ATTGTGGAAGATATTTATGTCTCTGGCGTAAAAGAAGAAAACATCACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGACT                                                                            >  SRR4302537.2863001/1‑94 (MQ=60)
                 ATTGTGGAAGATATTTATGTCTCTGGCGTAAAAGAAGAAAACATCACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGACT                                                                            >  SRR4302537.2834984/1‑94 (MQ=60)
                 ATTGTGGAAGATATTTATGTCTCTGGCGTAAAAGAAGAAAACATCACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGACT                                                                            >  SRR4302537.4190804/1‑94 (MQ=60)
                 ATTGTGGAAGATATTTATGTCTCTGGCGTAAAAGAAGAAAACATCACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGACT                                                                            <  SRR4302537.4718696/94‑1 (MQ=60)
                 ATTGTGGAAGATATTTATGTCTCTGGCGTAAAAGAAGAAAACATCACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGACGTGACT                                                                            >  SRR4302537.5002188/1‑94 (MQ=60)
                    GTGGAAGATATTTATGTCTCTGGCGTAAAAGAAGAAAACATCACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTATG                                                                         <  SRR4302537.2837602/94‑1 (MQ=60)
                     TGGAAGATATTTATGTCTCTGGCGTAAAAGAAGAAAACATCACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTATGA                                                                        <  SRR4302537.3699002/94‑1 (MQ=60)
                     TGGAAGATATTTATGTCTCTGGCGTAAAAGAAGAAAACATCACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTATGA                                                                        >  SRR4302537.5910934/1‑94 (MQ=60)
                      GGAAGATATTTATGTCTCTGGCGTAAAAGAAGAAAACATCACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTATGAC                                                                       <  SRR4302537.5691626/94‑1 (MQ=60)
                         AGATATTTATGTCTCTGGCGTAAAAGAAGAAAACATCACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTATGACACC                                                                    <  SRR4302537.1246546/94‑1 (MQ=60)
                         AGATATTTATGTCTCTGGCGTAAAAGAAGAAAACATCACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTATGACACC                                                                    >  SRR4302537.4602032/1‑94 (MQ=60)
                          GATATTTATGTCTCTGGCGTAAAAGAAGAAAACATCACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTATGACACCA                                                                   <  SRR4302537.474610/94‑1 (MQ=60)
                          GATATTTATGTCTCTGGCGTAAAAGAAGAAAACATCACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTATGACACCA                                                                   <  SRR4302537.2535157/94‑1 (MQ=60)
                          GATATTTATGTCTCTGGCGTAAAAGAAGAAAACATCACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTATGACACCA                                                                   >  SRR4302537.3294761/1‑94 (MQ=60)
                          GATATTTATGTCTCTGGCGTAAAAGAAGAAAACATCACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTATGACACCA                                                                   <  SRR4302537.3084674/94‑1 (MQ=60)
                          GATATTTATGTCTCTGGCGTAAAAGAAGAAAACATCACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTATGACACCA                                                                   >  SRR4302537.6349528/1‑94 (MQ=60)
                          GATATTTATGTCTCTGGCGTAAAAGAAGAAAACATCACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTATGACACCA                                                                   <  SRR4302537.2058875/94‑1 (MQ=60)
                          GATATTTATGTCTCTGGCGTAAAAGAAGAAAACATCACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTATGACACCA                                                                   >  SRR4302537.2187470/1‑94 (MQ=60)
                           ATATTTATGTCTCTGGCGTAAAAGAAGAAAACATCACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTATGACACCAG                                                                  >  SRR4302537.5971212/1‑94 (MQ=60)
                            TATTTATGTCTCTGGCGTAAAAGAAGAAAACATCACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTATGACACCAGT                                                                 <  SRR4302537.5551437/94‑1 (MQ=60)
                            TATTTATGTCTCTGGCGTAAAAGAAGAAAACATCACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTATGACACCAGT                                                                 >  SRR4302537.1040615/1‑94 (MQ=60)
                             ATTTATGTCTCTGGCGTAAAAGAAGAAAACATCACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTATGACACCAGTA                                                                >  SRR4302537.5097737/1‑94 (MQ=60)
                              TTTATGTCTCTGGCGTAAAAGAAGAAAACATCACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTATGACACCAGTAA                                                               <  SRR4302537.4913658/94‑1 (MQ=60)
                              TTTATGTCTCTGGCGTAAAAGAAGAAAACATCACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTATGACACCAGTAA                                                               <  SRR4302537.2776284/94‑1 (MQ=60)
                               TTATGTCTCTGGCGTAAAAGAAGAAAACATCACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAG                                                              >  SRR4302537.4766546/1‑94 (MQ=60)
                                TATGTCTCTGGCGTAAAAGAAGAAAACATCACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAGA                                                             <  SRR4302537.927756/94‑1 (MQ=60)
                                 ATGTCTCTGGCGTAAAAGAAGAAAACATCACTGTAGTAGGTGATGCGACCCCACAACCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAGAA                                                            <  SRR4302537.2042262/94‑1 (MQ=60)
                                   GTCTCTGGCGTAAAAGAAGAAAACATCACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAGAAAA                                                          >  SRR4302537.3406585/1‑94 (MQ=60)
                                    TCTCTGGCGTAAAAGAAGAAAACATCACTGTAGTAGGTGATGCGACACCACAACCCTCTTGCGTGGGCCGTGACTATGACACCAGTAAGAAAAT                                                         <  SRR4302537.4902058/94‑1 (MQ=60)
                                     CTCTGGCGTAAAAGAAGAAAACATCACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAGAAAATC                                                        >  SRR4302537.5222903/1‑94 (MQ=60)
                                     CTCTGGCGTAAAAGAAGAAAACATCACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAGAAAATC                                                        >  SRR4302537.3980624/1‑94 (MQ=60)
                                     CTCTGGCGTAAAAGAAGAAAACATCACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAGAAAATC                                                        >  SRR4302537.5222902/1‑94 (MQ=60)
                                       CTGGCGTAAAAGAAGAAAACATCACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAGAAAATCAC                                                      <  SRR4302537.5845680/94‑1 (MQ=60)
                                       CTGGCGTAAAAGAAGAAAACATCACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAGAAAATCAC                                                      <  SRR4302537.2499376/94‑1 (MQ=60)
                                       CTGGCGTAAAAGAAGAAAACATCACTGTAGTAGGGGATGCGACACCACAACCCTCTTACGTGGGCAGTGACTATGACACCAGTAAGAAAATAAC                                                      >  SRR4302537.1785294/1‑94 (MQ=60)
                                         GGCGTAAAAGAAGAAAACATCACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAGAAAATCACCG                                                    >  SRR4302537.5629367/1‑94 (MQ=60)
                                          GCGTAAAAGAAGAAAACATCACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAGAAAATCACCGA                                                   >  SRR4302537.2203799/1‑94 (MQ=60)
                                           CGTAAAAAAAGAAAACATCACTGTAGGAGGTGATGCGACCCCACAACCCTCTGCCGTGGGCCGTGACTATGACACCAGTAAGAAAATCACCGAA                                                  <  SRR4302537.1487051/94‑1 (MQ=60)
                                            GTAAAAGAAGAAAACATCACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAGAAAATCACCGAAC                                                 <  SRR4302537.3463062/94‑1 (MQ=60)
                                            GTAAAAGAAGAAAACATCACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAGAAAATCACCGAAC                                                 <  SRR4302537.3314405/94‑1 (MQ=60)
                                             TAAAAGAAGAAAACATCACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAGAAAATCACCGAACA                                                >  SRR4302537.4601524/1‑94 (MQ=60)
                                              AAAAGAAGAAAACATCACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAGAAAATCACCGAACAA                                               <  SRR4302537.2227231/94‑1 (MQ=60)
                                              AAAAGAAGAAAACATCACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAGAAAATCACCGAACAA                                               >  SRR4302537.600821/1‑94 (MQ=60)
                                              AAAAGAAGAAAACATCACTGTAGTAGGTAATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAGAAAATCACCGAACAA                                               >  SRR4302537.2541637/1‑94 (MQ=60)
                                               AAAGAAGAAAACATCACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAGAAAATCACCGAACAAA                                              <  SRR4302537.5527467/94‑1 (MQ=60)
                                               AAAGAAGAAAACATCACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAGAAAATCACCGAACAAA                                              >  SRR4302537.5213546/1‑94 (MQ=60)
                                                AAGAAGAAAACATCACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAGAAAATCACCGAACAAAG                                             <  SRR4302537.2099690/94‑1 (MQ=60)
                                                AAGAAGAAAACATCACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAGAAAATCACCGAACAAAG                                             <  SRR4302537.933381/94‑1 (MQ=60)
                                                 AGAAGAAAACATCACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAGAAAATCACCGAACAAAGC                                            >  SRR4302537.3402219/1‑94 (MQ=60)
                                                  GAAGAAAACATCACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAGAAAATCACCGAACAAAGCG                                           <  SRR4302537.2043062/94‑1 (MQ=60)
                                                  AAAGAAAACATCACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAGAAAATCACCGAACAAAGCG                                           <  SRR4302537.5010915/94‑1 (MQ=60)
                                                   AAGAAAACATCACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAGAAAATCACCGAACAAAGCGA                                          >  SRR4302537.6300281/1‑94 (MQ=60)
                                                    AGAAAACATCACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAGAAAATCACCGAACAAAGCGAT                                         >  SRR4302537.889751/1‑94 (MQ=60)
                                                    AGAAAACATCACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAGAAAATCACCGAACAAAGCGAT                                         >  SRR4302537.3321255/1‑94 (MQ=60)
                                                    AGAAAACATCACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAGAAAATCACCGAACAAAGCGAT                                         >  SRR4302537.4745251/1‑94 (MQ=60)
                                                     GAAAACATCACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAGAAAATCACCGAACAAAGCGATG                                        >  SRR4302537.5766751/1‑94 (MQ=60)
                                                     GAAAACATCACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAGAAAATCACCGAACAAAGCGATG                                        <  SRR4302537.1027969/94‑1 (MQ=60)
                                                     GAAAACATCACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAGAAAATCACCGAACAAAGCGATG                                        <  SRR4302537.4731406/94‑1 (MQ=60)
                                                      AAAACATCACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAGAAAATCACCGAACAAAGCGATGG                                       >  SRR4302537.2054584/1‑94 (MQ=60)
                                                      AAAACATCACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAGAAAATCACCGAACAAAGCGATGG                                       >  SRR4302537.4045364/1‑94 (MQ=60)
                                                       AAACATCACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAGAAAATCACCGAACAAAGCGATGGT                                      <  SRR4302537.6186524/94‑1 (MQ=60)
                                                         ACATCACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAGAAAATCACCGAACAAAGCGATGGTTT                                    <  SRR4302537.1291764/94‑1 (MQ=60)
                                                          CATCACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAGAAAATCACCGAACAAAGCGATGGTTTC                                   >  SRR4302537.1217238/1‑94 (MQ=60)
                                                          CATCACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAGAAAATCACCGAACAAAGCGATGGTTTA                                   >  SRR4302537.1017069/1‑94 (MQ=60)
                                                          CATCACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAGAAAATCACCGAACAAAGCGATGGTTTA                                   >  SRR4302537.5798372/1‑94 (MQ=60)
                                                          CATCACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAGAAAATCACCGAACAAAGCGATGGTTTA                                   >  SRR4302537.5663117/1‑94 (MQ=60)
                                                           ATCACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAGAAAATCACCGAACAAAGCGATGGTTTAC                                  <  SRR4302537.2350640/94‑1 (MQ=60)
                                                             CACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAGAAAATCACCGAACAAAGCGATGGTTTACTG                                <  SRR4302537.2570055/94‑1 (MQ=60)
                                                              ACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAGAAAATCACCGAACAAAGCGATGGTTTACTGG                               >  SRR4302537.3081021/1‑94 (MQ=60)
                                                                TGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAGAAAATCACCGAACAAAGCGATGGTTTACTGGCG                             >  SRR4302537.719474/1‑94 (MQ=60)
                                                                   AGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAGAAAATCACCGAACAAAGCGATGGTTTACTGGCGAAG                          >  SRR4302537.4233402/1‑94 (MQ=60)
                                                                   AGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAGAAAATCACCGAACAAAGCGATGGTTTACTGGCGAAG                          >  SRR4302537.3623994/1‑94 (MQ=60)
                                                                   AGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAGAAAATCACCGAACAAAGCGATGGTTTACTGGCGAAG                          <  SRR4302537.280258/94‑1 (MQ=60)
                                                                   AGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAGAAAATCACCGAACAAAGCGATGGTTTACTGGCGAAG                          <  SRR4302537.1328465/94‑1 (MQ=60)
                                                                    GTAGGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAGAAAATCACCGAACAAAGCGATGGTTTACTGGCGAAGG                         <  SRR4302537.1330076/94‑1 (MQ=60)
                                                                     TAGGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAGAAAATCACCGAACAAAGCGATGGTTTACTGGCGAAGGG                        >  SRR4302537.1586107/1‑94 (MQ=60)
                                                                       GGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAGAAAATCACCGAACAAAGCGATGGTTTACTGGCGAAGGTGG                      >  SRR4302537.4295479/1‑94 (MQ=60)
                                                                        GTGATGCGACACCACAGCCCTCTTCCGTGAGCCGTCACTATGACACCAGTAAGAAAATCTCCGAACAAAGCGATGGTTTACTGGCGAAGGTGGG                     <  SRR4302537.3693724/94‑1 (MQ=60)
                                                                        GTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAGAAAATCACCGAACAAAGCGATGGTTTACTGGCGAAGGTGGG                     <  SRR4302537.454142/94‑1 (MQ=60)
                                                                         TGATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAGAAAATCACCGAACAAAGCGATGGTTTACTGGCGAAGGTGGGA                    >  SRR4302537.438344/1‑94 (MQ=60)
                                                                         TGATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAGAAAATCACCGAACAAAGCGATGGTTTACTGGCGAAGGTGGGA                    >  SRR4302537.5722429/1‑94 (MQ=60)
                                                                           ATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAGAAAATCACCGAACAAAGCGATGGTTTACTGGCGAAGGTGGGAAT                  <  SRR4302537.2477459/94‑1 (MQ=60)
                                                                           ATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAGAAAATCACCGAACAAAGCGATGGTTTACTGGCGAAGGTGGGAAT                  >  SRR4302537.4554183/1‑94 (MQ=60)
                                                                           ATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAGAAAATCACCGAACAAAGCGATGGTTTACTGGCGAAGGTGGGAAT                  <  SRR4302537.527412/94‑1 (MQ=60)
                                                                               GACACCACAACCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAGAAAATCACCGAACAAAGCGATGGTTTACTGGCGAAGGTGGGAATGGGC              >  SRR4302537.479235/1‑94 (MQ=60)
                                                                                ACACCACAACCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAGAAAATCACCGAACAAAGCGATGGTTTACTGGCGAAGGTGGGAATGGGCA             <  SRR4302537.943181/94‑1 (MQ=60)
                                                                                ACACCACAACCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAGAAAATCACCGAACAAAGCGATGGTTTACTGGCGAAGGTGGGAATGGGCA             <  SRR4302537.4200529/94‑1 (MQ=60)
                                                                                   CCACAACCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAGAAAATCACCGAACAAAGCGATGGTTTACTGGCGAAGGTGGGAATGGGCATGG          >  SRR4302537.867089/1‑94 (MQ=60)
                                                                                   CCACAACCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAGAAAATCACCGAACAAAGCGATGGTTTACTGGCGAAGGTGGGAATGGGCATGG          <  SRR4302537.2907835/94‑1 (MQ=60)
                                                                                   CCACAACCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAGAAAATCACCGAACAAAGCGATGGATTACTGGCGAAGGGGGGAATGGGCATGG          >  SRR4302537.5802197/1‑94 (MQ=60)
                                                                                     ACAACCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAGAAAATCACCGAACAAAGCGATGGTTTACTGGCGAAGGTGGGAATGGGCATGGGG        <  SRR4302537.2088716/94‑1 (MQ=60)
                                                                                     ACAACCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAGAAAATCACCGAACAAAGCGATGGTTTACTGGCGAAGGTGGGAATGGGCATGGGG        >  SRR4302537.5121483/1‑94 (MQ=60)
                                                                                     ACAACCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAGAAAATCACCGAACAAAGCGATGGTTTACTGGCGAAGGTGGGAATGGGCATGGGG        >  SRR4302537.5121484/1‑94 (MQ=60)
                                                                                     ACAACCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAGAAAATCACCGAACAAAGCGATGGTTTACTGGCGAAGGTGGGAATGGGCATGGGG        <  SRR4302537.3059043/94‑1 (MQ=60)
                                                                                       AACCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAGAAAATCACCGAACAAAGCGATGGTTTACTGGCGAAGGTGGGAATGGGCATGGGGTC      <  SRR4302537.1802825/94‑1 (MQ=60)
                                                                                       AACCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAGAAAATCACCGAACAAAGCGATGGTTTACTGGCGAAGGTGGGAATGGGCATGGGGTC      >  SRR4302537.5531762/1‑94 (MQ=60)
                                                                                        ACCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAGAAAATCACCGAACAAAGCGATGGTTTACTGGCGAAGGTTGGAATGGGCATGGGGTCC     >  SRR4302537.268418/1‑94 (MQ=60)
                                                                                          CCTCTTCCGTGGGCCGTGACTATGACACCAGTAAGAAAATCACCGAACAAAGCGATGGTTTACTGGCGAAGGTGGGAATGGGCATGGGGTCCAC   <  SRR4302537.1059171/94‑1 (MQ=60)
                                                                                           CTCTTCCGTGGGCCGTGACTATGACACCAGTAAGAAAATCACCGAACAAAGCGATGGTTTACTGGCGAAGGTGGGAATGGGCATGGGGTCCACC  >  SRR4302537.2155759/1‑94 (MQ=60)
                                                                                             |                                                                                           
GTCCGCTGGCGCAGTACATTGTGGAAGATATTTATGTCTCTGGCGTAAAAGAAGAAAACATCACTGTAGTAGGTGATGCGACACCACAACCCTTTTCCGTGGGCCGTGACTATGACACCAGTAAGAAAATCACCGAACAAAGCGATGGTTTACTGGCGAAGGTGGGAATGGGCATGGGGTCCACC  >  NC_000913/2824704‑2824888

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 17 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑