Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A1 F1 I2 R1	882	254.4	12957588	98.0%	12698436	93.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,851,756	C→T	G146G (GGC→GGT)	hypE →	HypE

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,851,756	0	C	T	97.3%	761.7 / 3.4	222	G146G (GGC→GGT)	hypE	HypE
Reads supporting (aligned to +/- strand): ref base C (5/1); new base T (119/97); total (124/98)
Fisher's exact test for biased strand distribution p-value = 2.32e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.66e-01

Only 100 of 229 total aligned reads displayed.

GGCGATACTAAAGTGGTGCAGCGCGGCGCGGTAGATAAACTGTTTATCAACACCGCTGGCATGGGCGCAATTCCGGCGAATATTCACTGGGGCGCACAGACGCTAACCGCAGGCGATGTATTGCTGGTGAGCGGTACACTCGGCGACCACGGGGCGACTATCCTTAACCTGCGTGAGCAGCT  >  NC_000913/2851664‑2851845

ggCGATACTAAAGTGGTGCAGCGCGGCGCGGTAGATAAACTGTTTATCAACACCGCTGGCATGGGCGCAATTCCGGCGAATATTCACTGGGgc                                                                                           >  2:1539756/1‑93 (MQ=255)

   gATACTAAAGTGGTGCAGCGCGGCGCGGTAGATAAACTGTTTATCAACACCGCTGGCATGGGCGCAATTCCGGCGAATATTCACTGGGGTGca                                                                                        >  2:3691419/1‑93 (MQ=255)

      aCTAAAGTGGTGCAGCGCGGCGCGGTAGATAAACTGTTTATCAACACCGCTGGCATGGGCGCAATTCCGGCGAATATTCACTGGGGTGCACAg                                                                                     >  1:1362484/1‑93 (MQ=255)

       cTAAAGTGGTGCAGCGCGGCGCGGTAGATAAACTGTTTATCAACACCGCTGGCATGGGCGCAATTCCGGCGAATATTCACTGGGGTGCACAGa                                                                                    <  2:3386742/93‑1 (MQ=255)

        tAAAGTGGTGCAGCGCGGCGCGGTAGATAAACTGTTTATCAACACCGCTGGCATGGGCGCAATTCCGGCGAATATTCACTGGGGTGCACAGAc                                                                                   >  2:6249098/1‑93 (MQ=255)

         aaaGTGGTGCAGCGCGGCGCGGTAGATAAACTGTTTATCAACACCGCTGGCATGGGCGCAATTCCGGCGAATATTCACTGGGGTGCACAGACg                                                                                  <  1:2235413/93‑1 (MQ=255)

         aaaGTGGTGCAGCGCGGCGCGGTAGATAAACTGTTTATCAACACCGCTGGCATGGGCGCAATTCCGGCGAATATTCACTGGGGCGCACAGACg                                                                                  >  1:4036807/1‑93 (MQ=255)

          aaGTGGTGCAGCGCGGCGCGGTAGATAAACTGTTTATCAACACCGCTGGCATGGGCGCAATTCCGGCGAATATTCACTGGGGTGCACAGACGc                                                                                 <  1:4552131/93‑1 (MQ=255)

          aaGTGGTGCAGCGCGGCGCGGTAGATAAACTGTTTATCAACACCGCTGCCATGGGCGCAATTCCGGCGAATATTCACTGGGGTGCACAGACGc                                                                                 <  1:3877905/93‑1 (MQ=255)

            gtggtgCAGCGCGGCGCGGTAGATAAACTGTTTATCAACACCGCTGGCATGGGCGCAATTCCGGCGAATATTCACTGGGGTGCACAGACGCTa                                                                               >  1:3258037/1‑93 (MQ=255)

             tggtgCAGCGCGGCGCGGTAGATAAACTGTTTATCAACACCGCTGGCATGGGCGCAATTCCGGCGAATATTCACTGGGGTGCACAGACGCTaa                                                                              <  2:1412025/93‑1 (MQ=255)

             tggtgCAGCGCGGCGCGGTAGATAAACTGTTTATCAACACCGCTGGCATGGGCGCAATTCCGGCGAATATTCACTGGGGTGCACAGACGCTaa                                                                              <  1:2314276/93‑1 (MQ=255)

              ggtgCAGCGCGGCGCGTTAGATAAACTGTTTATCAACACCGCTGGCATGGGCGCAATTCCGGCGAATATTCACTGGGGTGCACAGACGCTAAc                                                                             <  2:3206965/93‑1 (MQ=255)

                  cAGCGCGGCGCGGTAGATAAACTGTTTATCAACACCGCTGGCATGGGCGCAATTCCGGCGAATATTCACTGGGGTGCACAGACGCTAACCGCa                                                                         <  2:2687491/93‑1 (MQ=255)

                   aGCGCGGCGCGGTAGATAAACTGTTTATCAACACCGCTGGCACGGGCGCAATTCCGGCGAATATTCACTGGGGTGCACAGACGCTAACCGCAg                                                                        <  1:554242/93‑1 (MQ=255)

                    gcgcggcgcgGTAGATAAACTGTTTATCAACACCGCTGGCATGGGCGCAATTCCGGCGAATATTCACTGGGGTGCACAGACGCTAACCGCAgg                                                                       >  2:2216961/1‑93 (MQ=255)

                    gcgcggcgcgGTAGATAAACTGTTTATCAACACCGCTGGCATGGGCGCAATTCCGGCGAATATTCACTGGGGTGCACAGACGCTAACCGCAgg                                                                       >  2:4379100/1‑93 (MQ=255)

                       cggcgcggTAGATAAACTGTTTATCAACACCGCTGGCATGGGCGCAATTCCGGCGAATATTCACTGGGGTGCACAGACGCTAACCGCAGGCGa                                                                    <  2:4880335/93‑1 (MQ=255)

                        ggcgcggTAGATAAACTGTTTATCAACACCGCTGGCATGGGCGCAATTCCGGCGAATATTCACTGGGGTGCACAGACGCTAACCGCAGGCGAt                                                                   >  2:6316222/1‑93 (MQ=255)

                         gcgcggTAGATAAACTGTTTATCAACACCGCTGGCATGGGCGCAATTCCGGCGAATATTCACTGGGGTGCACAGACGCTAACCGCAGGCGATg                                                                  >  2:6280538/1‑93 (MQ=255)

                         gcgcggTAGATAAACTGTTTATCAACACCGCTGGCATGGGCGCAATTCCGGCGAATATTCACTGGGGTGCACAGACGCTAACCGCAGGCGATg                                                                  >  2:5712042/1‑93 (MQ=255)

                         gcgcggTAGATAAACTGTTTATCAACACCGCTGGCATGGGCGCAATTCCGGCGAATATTCACTGGGGTGCACAGACGCTAACCGCAGGCGATg                                                                  <  2:4895027/93‑1 (MQ=255)

                            cggTAGATAAACTGTTTATCAACACCGCTGGCATGGGCGCAATTCCGGCGAATATTCACTGGGGTGCACAGACGCTAACCGCAGGCGATGTAt                                                               <  2:1028820/93‑1 (MQ=255)

                             ggTAGATAAACTGTTTATCAACACCGCTGGCATGGGCGCAATTCCGGCGAATATTCACTGGGGTGCACAGACGCTAACCGCAGGCGATGTAtt                                                              <  2:4364189/93‑1 (MQ=255)

                             ggTAGATAAACTGTTTATCAACACCGCTGGCATGGGCGCAATTCCGGCGAATATTCACTGGGGTGCACAGACGCTAACCGCAGGCGATGTAtt                                                              >  2:5540163/1‑93 (MQ=255)

                              gTAGATAAACTGTTTATCAACACCGCTGGCATGGGCGCAATTCCGGCGAATATTCACTGGGGTGCACAGACGCTAACCGCAGGCGATGTATTg                                                             >  2:2961140/1‑93 (MQ=255)

                               tAGATAAACTGTTTATCAACACCGCTGGCATGGGCGCAATTCCGGCGAATATTCACTGGGGTGCACAGACGCTAACCGCAGGCGATGTATTGc                                                            <  1:2641600/93‑1 (MQ=255)

                                 gATAAACTGTTTATCAACACCGCTGGCATGGGCGCAATTCCGGCGAATATTCACTGGGGTGCACAGACGCTAACCGCAGGCGATGTATTGCTg                                                          >  2:250324/1‑93 (MQ=255)

                                 gATAAACTGTTTATCAACACCGCTGGCATGGGCGCAATTCCGGCGAATATTCACTGGGGTGCACAGACGCTAACCGCAGGCGATGTATTGCTg                                                          <  1:1042175/93‑1 (MQ=255)

                                  aTAAACTGTTTATCAACACCGCTGGCATGGGCGCAATTCCGGCGAATATTCACTGGGGTGCACAGACGCTAACCGCAGGCGATGTATTGCTgg                                                         >  1:4609120/1‑93 (MQ=255)

                                   tAAACTGTTTATCAACACCGCTGGCATGGGCGCAATTCCGGCGAATATTCACTGGGGTGCACAGACGCTAACCGCAGGCGATGTATTGCTGGt                                                        >  2:5047039/1‑93 (MQ=255)

                                   tAAACTGTTTATCAACACCGCTGGCATGGGCGCAATTCCGGCGAATATTCACTGGGGTGCACAGACGCTAACCGCAGGCGATGTATTGCTGGt                                                        >  2:2009028/1‑93 (MQ=255)

                                   tAAACTGTTTATCAACACCGCTGGCATGGGCGCAATTCCGGCGAATATTCACTGGGGTGCACAGACGCTAACCGCAGGCGATGGATTGCTGGt                                                        >  2:115204/1‑93 (MQ=255)

                                   tAAACTGTTTATCAACACCGCTGGCATGGGCGCAATTCCGGCGAATATTCACTGGGGTGCACAGACGCTAACCGCAGGCGATGGATTGCTGGt                                                        >  2:5191224/1‑93 (MQ=255)

                                    aaaCTGTTTATCAACACCGCTGGCATGGGCGCAATTCCGGCGAATATTCACTGGGGTGCACAGACGCTAACCGCAGGCGATGTATTGCTGGTg                                                       >  2:6010424/1‑93 (MQ=255)

                                    aaaCTGTTTATCAACACCGCTGGCATGGGCGCAATTCCGGCGAATATTCACTGGGGTGCACAGACGCTAACCGCAGGCGATGTATTGCTGGTg                                                       >  1:5843523/1‑93 (MQ=255)

                                     aaCTGTTTATCAACACCGCTGGCATGGGCGCAATTCCGGCGAATATTCACTGGGGTGCACAGACGCTAACCGCAGGCGATGTATTGCTGGTGa                                                      <  1:4999433/93‑1 (MQ=255)

                                      aCTGTTTATCAACACCGCTGGCATGGGCGCAATTCCGGCGAATATTCACTGGGGTGCACAGACGCTAACCGCAGGCGATGTATTGCTGGTGAg                                                     >  1:812912/1‑93 (MQ=255)

                                       cTGTTTATCAACACCGCTGGCATGGGCGCAATTCCGGCGAATATTCACTGGGGTGCACAGACGCTAACCGCAGGCGATGTATTGCTGGTGAGc                                                    <  1:4957360/93‑1 (MQ=255)

                                       cTGTTTATCAACACCGCTGGCATGGGCGCAATTCCGGCGAATATTCACTGGGGTGCACAGACGCTAACCGCAGGCGATGTATTGCTGGTGAGc                                                    <  1:3228935/93‑1 (MQ=255)

                                        tGTTTATCAACACCGCTGGCATGGGCGCAATTCCGGCGAATATTCACTGGGGTGCACAGACGCTAACCGCAGGCGATGTATTGCTGGTGAGCg                                                   >  2:1539433/1‑93 (MQ=255)

                                           ttATCAACACCGCTGGCATGGGCGCAATTCCGGCGAATATTCACTGGGGTGCACAGACGCTAACCGCAGGCGATGTATTGCTGGTGAGCGGTa                                                >  2:3270228/1‑93 (MQ=255)

                                           ttATCAACACCGCTGGCATGGGCGCAATTCCGGCGAATATTCACTGGGGTGCACAGACGCTAACCGCAGGCGATGTATTGCTGGTGAGCGGTa                                                <  2:5379923/93‑1 (MQ=255)

                                           ttATCAACACCGCTGGCATGGGCGCAATTCCGGCGAATATTCACTGGGGTGCACAGACGCTAACCGCAGGCGATGTATTGCTGGTGAGCGGTa                                                >  1:3390037/1‑93 (MQ=255)

                                            tATCAACACCGCTGGCATGGGCGCAATTCCGGCGAATATTCACTGGGGTGCACAGACGCTAACCGCAGGCGATGTATTGCTGGTGAGCGGTac                                               >  1:5343882/1‑93 (MQ=255)

                                               cAACACCGCTGGCATGGGCGCAATTCCGGCGAATATTCACTGGGGTGCACAGACGCTAACCGCAGGCGATGTATTGCTGGTGAGCGGTACACt                                            >  1:3122777/1‑93 (MQ=255)

                                               cAACACCGCTGGCATGGGCGCAATTCCGGCGAATATTCACTGGGGTGCACAGACGCTAACCGCAGGCGATGTATTGCTGGTGAGCGGTACACt                                            >  1:1992995/1‑93 (MQ=255)

                                                aaCACCGCTGGCATGGGCGCAATTCCGGCGAATATTCACTGGGGTGCACAGACGCTAACCGCAGGCGATGTATTGCTGGTGAGCGGTACACTc                                           <  1:1559766/93‑1 (MQ=255)

                                                aaCACCGCTGGCATGGGCGCAATTCCGGCGAATATTCACTGGGGTGCACAGACGCTAACCGCAGGCGATGTATTGCTGGTGAGCGGTACACTc                                           <  1:6187630/93‑1 (MQ=255)

                                                 acacCGCTGGCATGGGCGCAATTCCGGCGAATATTCACTGGGGTGCACAGACGCTAACCGCAGGCGATGTATTGCTGGTGAGCGGTACACTCg                                          >  2:3916293/1‑93 (MQ=255)

                                                  cacCGCTGGCATGGGCGCAATTCCGGCGAATATTCACTGGGGCGCACAGACGCTAACCGCAGGCGATGTATTGCTGGTGAGCGGTACACCCgg                                         >  1:1285626/1‑93 (MQ=255)

                                                    ccGCTGGCATGGGCGCAATTCCGGCGAATATTCACTGGGGTGCACAGACGCTAACCGCAGGCGATGTATTGCTGGTGAGCGGTACACTCGGCg                                       >  1:1599537/1‑93 (MQ=255)

                                                     cGCTGGCATGGGCGCAATTCCGGCGAATATTCACTGGGGTGCACAGACGCTAACCGCAGGCGATGTATTGCTGGTGAGCGGTACACTCGGCGa                                      <  1:5017509/93‑1 (MQ=255)

                                                      gCTGGCATGGGCGCAATTCCGGCGAATATTCACTGGGGTGCACAGACGCTAACCGCAGGCGATGTATTGCTGGTGAGCGGTACACTCGGCGAc                                     >  2:1375413/1‑93 (MQ=255)

                                                      gCTGGCATGGGCGCAATTCCGGCGAATATTCACTGGGGTGCACAGACGCTAACCGCAGGCGATGTATTGCTGGTGAGCGGTACACTCGGCGAc                                     <  1:5219758/93‑1 (MQ=255)

                                                       cTGGCATGGGCGCAATTCCGGCGAATATTCACTGGGGTGCACAGACGCTCACCGCAGGCGATGTATTGCTGGTGAGCGGTACACTCGGCGAcc                                    >  2:6379110/1‑93 (MQ=255)

                                                       cTGGCATGGGCGCAATTCCGGCGAATATTCACTGGGGTGCACAGACGCTAACCGCAGGCGATGTATTGCTGGTGAGCGGTACACTCGGCGAcc                                    >  1:3606838/1‑93 (MQ=255)

                                                       cTGGCATGGGCGCAATTCCGGCGAATATTCACTGGGGTGCACAGACGCTAACCGCAGGCGATGTATTGCTGGTGAGCGGTACACTCGGCGAcc                                    >  2:689122/1‑93 (MQ=255)

                                                        tGGCATGGGCGCAATTCCGGCGAATATTCACTGGGGTGCACAGACGCTAACCGCAGGCGATGTATTGCTGGTGAGCGGTACACTCGGCGACCa                                   <  2:4070815/93‑1 (MQ=255)

                                                        tGGCATGGGCGCAATTCCGGCGAATATTCACTGGGGTGCACAGACGCTAACCGCAGGCGATGTATTGCTGGTGAGCGGTACACTCGGCGACCa                                   >  1:2902391/1‑93 (MQ=255)

                                                        tGGCATGGGCGCAATTCCGGCGAATATTCACTGGGGCGCACAGACGCTAACCGCAGGCGATGCATTGCTGGTGAGCGGTACACTCGGCGACCa                                   >  1:4065398/1‑93 (MQ=255)

                                                         ggCATGGGCGCAATTCCGGCGAATATTCACTGGGGTGCACAGACGCTAACCGCAGGCGATGTATTGCTGGTGAGCGGTACACTCGGCGACCAc                                  >  1:330614/1‑93 (MQ=255)

                                                          gCATGGGCGCAATTCCGGCGAATATTCACTGGGGTGCACAGACGCTAACCGCAGGCGATGTATTGCTGGTGAGCGGTACACTCGGCGACCACg                                 <  2:1695267/93‑1 (MQ=255)

                                                          gCATGGGCGCAATTCCGGCGAATATTCACTGGGGTGCACAGACGCTAACCGCAGGCGATGTATTGCTGGTGAGCGGTACACTCGGCGACCACg                                 <  1:845766/93‑1 (MQ=255)

                                                           cATGGGCGCAATTCCGGCGAATATTCACTGGGGTGCACAGACGCTAACCGCAGGCGATGTATTGCTGGTGAGCGGTACACTCGGCGACCACgg                                <  2:427014/93‑1 (MQ=255)

                                                           cATGGGCGCAATTCCGGCGAATATTCACTGGGGTGCACAGACGCTAACCGCAGGCGATGTATTGCTGGTGAGCGGTACACTCGGCGACCACgg                                <  2:2932853/93‑1 (MQ=255)

                                                            aTGGGCGCAATTCCGGCGAATATTCACTGGGGTGCACAGACGCTAACCGCAGGCGATGTATTGCTGGTGAGCGGTACACTCGGCGACCACggg                               >  2:6395773/1‑93 (MQ=255)

                                                            aTGGGCGCAATTCCGGCGAATATTCACTGGGGTGCACAGACGCTAACCGCAGGCGATGTATTGCTGGTGAGCGGTACACTCGGCGACCACggg                               >  2:3251634/1‑93 (MQ=255)

                                                             tGGGCGCAATTCCGGCGAATATTCACTGGGGTGCACAGACGCTAACCGCAGGCGATGTATTGCTGGTGAGCGGTACACTCGGCGACCACgggg                              >  2:2882137/1‑93 (MQ=255)

                                                             tGGGCGCAATTCCGGCGAATATTCACTGGGGTGCACAGACGCTAACCGCAGGCGATGTATTGCTGGTGAGCGGTACACTCGGCGACCACgggg                              <  1:5998945/93‑1 (MQ=255)

                                                                 cgcAATTCCGGCGAATATTCACTGGGGTGCACAGACGCTAACCGCAGGCGATGTATTGCTGGTGAGCGGTACACTCGGCGACCACGGGGCGAc                          <  2:3735212/93‑1 (MQ=255)

                                                                 cgcAATTCCGGCGAATATTCACTGGGGTGCACAGACGCTAACCGCAGGCGATGTATTGCTGGTGAGCGGTACACTCGGCGACCACGGGGCGAc                          >  1:4386055/1‑93 (MQ=255)

                                                                  gcAATTCCGGCGAATATTCACTGGGGTGCACAGACGCTAACCGCAGGCGATGTATTGCTGGTGAGCGGTACACTCGGCGACCACGGGGCGACt                         >  2:1300962/1‑93 (MQ=255)

                                                                   cAATTCCGGCGAATATTCACTGGGGCGCACAGACGCTAACCGCAGGCGATGTATTGCTGGTGAGCGGTACACTCGGCGACCACGGGGCGACTa                        >  1:1724911/1‑93 (MQ=255)

                                                                     aTTCCGGCGAATATTCACTGGGGTGCACAGACGCTAACCGCAGGCGATGTATTGCTGGTGAGCGGTACACTCGGCGACCACGGGGCGACTATc                      <  1:2779176/93‑1 (MQ=255)

                                                                     aTTCCGGCGAATATTCACTGGGGTGCACAGACGCTAACCGCAGGCGATGTATTGCTGGTGAGCGGTACACTCGGCGACCACGGGGCGACTATc                      >  2:1203497/1‑93 (MQ=255)

                                                                         cGGCGAATATTCACTGGGGTGCACAGACGCTAACCGCAGGCGATGTATTGCTGGTGAGCGGTACACTCGGCGACCACGGGGCGACTATCCTTa                  >  1:1058062/1‑93 (MQ=255)

                                                                         cGGCGAATATTCACTGGGGTGCACAGACGCTAACCGCAGGCGATGTATTGCTGGTGAGCGGTACACTCGGCGACCACGGGGCGACTATCCTTa                  <  1:3441051/93‑1 (MQ=255)

                                                                         cGGCGAATATTCACTGGGGTGCACAGACGCTAACCGCAGGCGATGTATTGCTGGTGAGCGGTACACTCGGCGACCACGGGGCGACTATCCTTa                  <  1:5473908/93‑1 (MQ=255)

                                                                          ggggAATATTCGCTGGGGTGCAAAGACGTTAACCGCAGGCGATGTATTGCTGGTTAGCGGTACACTCGGCGAGCAGGGGGCGAGTATCCTTaa                 <  2:4597287/90‑1 (MQ=255)

                                                                          ggCGAATATTCACTGGGGTGCACAGACGCTAACCGCAGGCGATGTATTGCTGGTGAGCGGTACACTCGGCGACCACGGGGCGACTATCCTTaa                 <  2:2700617/93‑1 (MQ=255)

                                                                             gAATATTCACTGGGGTGCACAGACGCTAACCGCAGGCGATGTATTGCTGGTGAGCGGTACACTCGGCGACCACGGGGCGACTATCCTTAACCt              >  1:2424349/1‑93 (MQ=255)

                                                                              aaTATTCACTGGGGTGCACAGACGCTAACCGCAGGCGATGTATTGCTGGTGAGCGGTACACTCGGCGACCACGGGGCGACTATCCTTAACCTg             >  1:754443/1‑93 (MQ=255)

                                                                              aaTATTCACTGGGGTGCACAGACGCTAACCGCAGGCGATGTATTGCTGGTGAGCGGTACACTCGGCGACCACGGGGCGACTATCCTTAACCTg             >  2:1927819/1‑93 (MQ=255)

                                                                              aaTATTCACTGGGGTGCACAGACGCTAACCGCAGGCGATGTATTGCTGGTGAGCGGTACACTCGGCGACCACGAGGCGACTATCCTTAACCTg             >  2:3980755/1‑93 (MQ=255)

                                                                               atatTCACTGGGGTGCACAGACGCTAACCGCAGGCGATGTATTGCTGGTGAGCGGTACACTCGGCGACCACGGGGCGACTATCCTTAACCTGc            >  2:2749813/1‑93 (MQ=255)

                                                                               atatTCACTGGGGTGCACAGACGCTAACCGCAGGCGATGTATTGCTGGTGAGCGGTACACTCGGCGACCACGGGGCGACTATCCTTAACCTGc            <  1:1435201/93‑1 (MQ=255)

                                                                               atatTCACTGGGGTGCACAGACGCTAACCGCAGGCGATGTATTGCTGGTGAGCGGTACACTCGGCGACCACGGGGCGACTATCCTTAACCTGc            <  2:5248617/93‑1 (MQ=255)

                                                                                tatTCACTGGGGTGCACAGACGCTAACCGCAGGCGATGTATTGCTGGTGAGCGGTACACTCGGCGACCACGGGGCGACTATCCTTAACCTGCg           <  2:418757/93‑1 (MQ=255)

                                                                                 atTCACTGGGGTGCACAGACGCTAACCGCAGGCGATGTATTGCTGGTGAGCGGTACACTCGGCGACCACGGGGCGACTATCCTTAACCTGCGt          >  2:182168/1‑93 (MQ=255)

                                                                                  ttCACTGGGGTGCACAGACGCTAACCGCAGGCGATGTATTGCTGGTGAGCGGTACACTCGGCGACCACGGGGCGACTATCCTTAACCTGCGTg         >  1:6363402/1‑93 (MQ=255)

                                                                                  ttCACTGGGGTGCACAGACGCTAACCGCAGGCGATGTATTGCTGGTGAGCGGTACACTCGGCGACCACGGGGCGACTATCCTTAACCTGCGTg         >  1:2252791/1‑93 (MQ=255)

                                                                                  ttCACTGGGGTGCACAGACGCTAACCGCAGGCGATGTATTGCTGGTGAGCGGTACACTCGGCGACCACGGGGCGACTATCCTTAACCTGCGTg         <  1:2826006/93‑1 (MQ=255)

                                                                                     aCTGGGGTGCACAGACGCTAACCGCAGGCGATGTATTGCTGGTGAGCGGTACACTCGGCGACCACGGGGCGACTATCCTTAACCTGCGTGagc      <  2:1481321/93‑1 (MQ=255)

                                                                                     aCTGGGGTGCACAGACGCTAACCGCAGGCGATGTATTGCTGGTGAGCGGTACACTCGGCGACCACGGGGCGACTATCCTTAACCTGCGTGagc      >  2:23237/1‑93 (MQ=255)

                                                                                      cTGGGGTGCACAGACGCTAACCGCAGGCGATGTATTGCTGGTGAGCGGTACACTCGGCGACCACGGGGCGACTATCCTTAACCTGCGTGagca     >  2:4692312/1‑93 (MQ=255)

                                                                                       tGGGGTGCACAGACGCTAACCGCAGGCGATGTATTGCTGGTGAGCGGTACACTCGGCGACCACGGGGCGACTATCCTTAACCTGCGTGagcag    >  1:64534/1‑93 (MQ=255)

                                                                                       tGGGGTGCACAGACGCTAACCGCAGGCGATGTATTGCTGGTGAGCGGTACACTCGGCGACCACGGGGCGACTATCCTTAACCTGCGTGagcag    >  1:918531/1‑93 (MQ=255)

                                                                                        ggggTGCACAGACGCTAACCGCAGGCGATGTATTGCTGGTGAGCGGTACACTCGGCGACCACGGGGCGACTATCCTTCACCTGCGTGagcagc   >  1:4216182/1‑93 (MQ=255)

                                                                                         gggTGCACAGACGCTAACCGCAGGCGATGTATTGCTGGTGAGCGGTACACTCGGCGACCACGGGGCGACTATCCTTAACCTGCGTGAGCAGCt  >  2:3575753/1‑93 (MQ=255)

GGCGATACTAAAGTGGTGCAGCGCGGCGCGGTAGATAAACTGTTTATCAACACCGCTGGCATGGGCGCAATTCCGGCGAATATTCACTGGGGCGCACAGACGCTAACCGCAGGCGATGTATTGCTGGTGAGCGGTACACTCGGCGACCACGGGGCGACTATCCTTAACCTGCGTGAGCAGCT  >  NC_000913/2851664‑2851845

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 20 ≤` `ATCG/ATCG` `< 29 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

BRESEQ :: bam2aln output

TGGCGATACTAAAGTGGTGCAGCGCGGCGCGGTAGATAAACTGTTTATCAACACCGCTGGCATGGGCGCAATTCCGGCGAATATTCACTGGGGCGCACAGACGCTAACCGCAGGCGATGTATTGCTGGTGAGCGGTACACTCGGCGACCACGGGGCGACTATCCTTAACCTGCGTGAGCAGCTGGGG  >  NC_000913/2851663‑2851849

TGGCTATACTAAAGTGGTGCAGCGCGGCGCGGTAGATAAACTGTTTATCAACACCGCTGGCATGGGCGCAATTCCGGCGAATATTCACTGGGGT                                                                                               <  SRR4302537.1236809/94‑1 (MQ=60)

 GGCGATACTAAAGTGGTGCAGCGCGGCGCGGTAGATAAACTGTTTATCAACACCGCTGGCATGGGCGCAATTCCGGCGAATATTCACTGGGGTG                                                                                              >  SRR4302537.3385776/1‑94 (MQ=60)

 GGCGATACTAAAGTGGTGCAGCGCGGCGCGGTAGATAAACTGTTTATCAACACCGCTGGCATGGGCGCAATTCCGGCGAATATTCACTGGGGTG                                                                                              >  SRR4302537.3755915/1‑94 (MQ=60)

   CGATACTAAAGTGGTGCAGCGCGGCGCGGTAGATAAACTGTTTATCAACACCGCTGGCATGGGCGCAATTCCGGCGAATATTCACTGGGGTGCA                                                                                            >  SRR4302537.2779724/1‑94 (MQ=60)

   CGATACTAAAGTGGTGCAGCGCGGCGCGGTAGATAAACTGTTTATCAACACCGCTGGCATGGGCGCAATTCCGGCGAATATTCACTGGGGTGCA                                                                                            <  SRR4302537.6026876/94‑1 (MQ=60)

   CGATACTAAAGTGGTGCAGCGCGGCGCGGTAGATAAACTGTTTATCAACACCGCTGGCATGGGCGCAATTCCGGCGAATATTCACTGGGGTGCA                                                                                            >  SRR4302537.4417622/1‑94 (MQ=60)

    GATACTAAAGTGGTGCAGCGCGGCGCGGTAGATAAACTGTTTATCAACACCGCTGGCATGGGCGCAATTCCGGCGAATATTCACTGGGGTGCAC                                                                                           >  SRR4302537.909186/1‑94 (MQ=60)

      TACTAAAGTGGTGCAGCGCGGCGCGGTAGATAAACTGTTTATCAACACCGCTGGCATGGGCGCAATTCCGGCGAATATTCACTGGGGTGCACAG                                                                                         >  SRR4302537.3917951/1‑94 (MQ=60)

      TACTAAAGTGGTGCAGCGCGGCGCGGTAGATAAACTGTTTATCAACACCGCTGGCATGGGCGCAATTCCGGCGAATATTCACTGGGGTGCACAG                                                                                         >  SRR4302537.1371975/1‑94 (MQ=60)

         TAAAGTGGTGCAGCGCGGCGCGGTAGATAAACTGTTTATCAACACCGCTGGCATGGGCGCAATTCCGGCGAATATTCACTGGGGTGCACAGACG                                                                                      <  SRR4302537.769033/94‑1 (MQ=60)

         TAAAGTGGTGCAGCGCGGCGCGGTAGATAAACTGTTTATCAACACCGCTGGCATGGGCGCAATTCCGGCGAATATTCACTGGGGCGCACAGACG                                                                                      >  SRR4302537.4065056/1‑94 (MQ=60)

          AAAGTGGTGCAGCGCGGCGCGGTAGATAAACTGTTTATCAACACCGCTGGCATGGGCGCAATTCCGGCGAATATTCACTGGGGTGCACAGACGC                                                                                     >  SRR4302537.3411781/1‑94 (MQ=60)

          AAAGTGGTGCAGCGCGGCGCGGTAGATAAACTGTTTATCAACACCGCTGGCATGGGCGCAATTCCGGCGAATATTCACTGGGGTGCACAGACGC                                                                                     <  SRR4302537.2251179/94‑1 (MQ=60)

           AAGTGGTGCAGCGCGGCGCGGTAGATAAACTGTTTATCAACACCGCTGGCATGGGCGCAATTCCGGCTAATATTCACTGGGGTGCACAGACGCT                                                                                    <  SRR4302537.2258985/94‑1 (MQ=60)

           AAGTGGTGCAGCGCGGCGCGGTAGATAAACTGTTTATCAACACCGCTGGCATGGGCGCAATTCCGGCGAATATTCACTGGGGTGCACAGACGCT                                                                                    <  SRR4302537.4583630/94‑1 (MQ=60)

           AAGTGGTGCAGCGCGGCGCGGTAGATAAACTGTTTATCAACACCGCTGCCATGGGCGCAATTCCGGCGAATATTCACTGGGGTGCACAGACGCT                                                                                    <  SRR4302537.3905027/94‑1 (MQ=60)

            AGTGGTGCAGCGCGGCGCGGTAGATAAACTGTTTATCAACACCGCTGGCATGGGCGCAATTCCGGCGAATATTCACTGGGGTGCACAGACGCTA                                                                                   >  SRR4302537.3281006/1‑94 (MQ=60)

              TGGTGCAGCGCGGCGCGGTAGATAAACTGTTTATCAACACCGCTGGCATGGGCGCAATTCCGGCGAATATTCACTGGGGTGCACAGACGCTAAC                                                                                 <  SRR4302537.2330611/94‑1 (MQ=60)

                  GCAGCGCGGCGCGGTAGATAAACTGTTTATCAACACCGCTGGCATGGGCGCAATTCCGGCGAATATTCACTGGGGTGCACAGACGCTAACCGCA                                                                             <  SRR4302537.5756998/94‑1 (MQ=60)

                   CAGCGCGGCGCGGTAGATAAACTGTTTATCAACACCGCTGGCATGGGCGCAATTCCGGCGAATATTCACTGGGGTGCACAGACGCTAACCGCAG                                                                            <  SRR4302537.92681/94‑1 (MQ=60)

                    AGCGCGGCGCGGTAGATAAACTGTTTATCAACACCGCTGGCATGGGCGCAATTCCGGCGAATATTCACTGGGGTGCACAGACGCTAACCGCAGG                                                                           >  SRR4302537.232503/1‑94 (MQ=60)

                    AGCGCGGCGCGGTAGATAAACTGTTTATCAACACCGCTGGCATGGGCGCAATTCCGGCGAATATTCACTGGGGTGCACAGACGCTAACCGCAGG                                                                           <  SRR4302537.1472934/94‑1 (MQ=60)

                    AGCGCGGCGCGGTAGATAAACTGTTTATCAACACCGCTGGCACGGGCGCAATTCCGGCGAATATTCACTGGGGTGCACAGACGCTAACCGCAGG                                                                           <  SRR4302537.558056/94‑1 (MQ=60)

                     GCGCGGCGCGGTAGATAAACTGTTTATCAACACCGCTGGCATGGGCGCAATTCCGGCGAATATTCACTGGGGTGCACAGACGCTAACCGCAGGC                                                                          <  SRR4302537.3096488/94‑1 (MQ=60)

                     GCGCGGCGCGGTAGATAAACTGTTTATCAACACCGCTGGCATGGGCGCAATTCCGGCGAATATTCACTGGGGTGCACAGACGCTAACCGCAGGC                                                                          <  SRR4302537.265879/94‑1 (MQ=60)

                       GCGGCGCGGTAGATAAACTGTTTATCAACACCGCTGGCATGGGCGCAATTCCGGCGAATATTCACTGGGGTGCACAGACGCTAACCGCAGGCGA                                                                        >  SRR4302537.4301851/1‑94 (MQ=60)

                        CGGCGCGGTAGATAAACTGTTTATCAACACCGCTGGCATGGGCGCAATTCCGGCGAATATTCACTGGGGTGCACAGACGCTAACCGCAGGCGAT                                                                       >  SRR4302537.5627600/1‑94 (MQ=60)

                         GGCGCGGTAGATAAACTGTTTATCAACACCGCTGGCATGGGCGCAATTCCGGCGAATATTCACTGGGGTGCACAGACGCTAACCGCAGGCGATG                                                                      >  SRR4302537.3772097/1‑94 (MQ=60)

                             CGGTAGATAAACTGTTTATCAACACCGCTGGCATGGGCGCAATTCCGGCGAATATTCACTGGGGTGCACAGACGCTAACCGCAGGCGATGTATT                                                                  >  SRR4302537.4183802/1‑94 (MQ=60)

                                TAGATAAACTGTTTATCAACACCGCTGGCATGGGCGCAATTCCGGCGAATATTCACTGGGGTGCACAGACGCTAACCGCAGGCGATGTATTGCT                                                               <  SRR4302537.2660082/94‑1 (MQ=60)

                                  GATAAACTGTTTATCAACACCGCTGGCATGGGCGCAATTCCGGCGAATATTCACTGGGGTGCACAGACGCTAACCGCAGGCGATGTATTGCTGG                                                             <  SRR4302537.1049373/94‑1 (MQ=60)

                                  GATAAACTGTTTATCAACACCGCTGGCATGGGCGCAATTCCGGCGAATATTCACTGGGGTGCACAGACGCTAACCGCAGGCGATGTATTGCTGG                                                             >  SRR4302537.4641033/1‑94 (MQ=60)

                                   ATAAACTGTTTATCAACACCGCTGGCATGGGCGCAATTCCGGCGAATATTCACTGGGGTGCACAGACGCTAACCGCAGTCGATGTATTGCTGGT                                                            <  SRR4302537.1182076/94‑1 (MQ=60)

                                   ATAAACTGTTTATCAACACCGCTGGCATGGGCGCAATTCCGGCGAATATTCACTGGGGTGCACAGACGCTAACCGCAGGCGATGTATTGCTGGT                                                            >  SRR4302537.1638816/1‑94 (MQ=60)

                                   ATAAACTGTTTATCAACACCGCTGGCATGGGCGCAATTCCGGCGAATATTCACTGGGGTGCACAGACGCTAACCGCAGGCGATGTATTGCTGGT                                                            <  SRR4302537.2472354/94‑1 (MQ=60)

                                   ATAAACTGTTTATCAACACCGCTGGCATGGGCGCAATTCCGGCGAATATTCACTGGGGTGCACAGACGCTAACCGCAGGCGATGTATTGCTGGT                                                            <  SRR4302537.3376422/94‑1 (MQ=60)

                                    TAAACTGTTTATCAACACCGCTGGCATGGGCGCAATTCCGGCGAATATTCACTGGGGTGCACAGACGCTAACCGCAGGCGATGTATTGCTGGTG                                                           >  SRR4302537.5883590/1‑94 (MQ=60)

                                      AGCTGTTTATCAACACCGCTGGCATGGGCGCAATTCCGGCGAATATTCACTGGGGTGCACAGACGCTAACCGCAGGCGATGTATTGCTGGTGAG                                                         <  SRR4302537.1574238/94‑1 (MQ=60)

                                      AACTGTTTATCAACACCGCTGGCATGGGCGCAATTCCGGCGAATATTCACTGGGGTGCACAGACGCTAACCGCAGGCGATGTATTGCTGGTGAG                                                         <  SRR4302537.5033880/94‑1 (MQ=60)

                                      AACTGTTTATCAACACCGCTGGCATGGGCGCAATTCCGGCGAATATTCACTGGGGTGCACAGACGCTAACCGCAGGCGATGTATTGCTGGTGAG                                                         >  SRR4302537.818482/1‑94 (MQ=60)

                                       ACTGTTTATCAACACCGCTGGCATGGGCGCAATGCCGGCGAATATTCACTGGGGTGCACAGACGCTAACCGCAGGCGATGTATTGCTGGTGAGC                                                        <  SRR4302537.633492/94‑1 (MQ=60)

                                        CTGTTTATCAACACCGCTGGCATGGGCGCAATTCCGGCGAATATTCACTGGGGTGCACAGACGCTAACCGCAGGCGATGTATTGCTGGTGAGCG                                                       <  SRR4302537.4991535/94‑1 (MQ=60)

                                        CTGTTTATCAACACCGCTGGCATGGGCGCAATTCCGGCGAATATTCACTGGGGTGCACAGACGCTAACCGCAGGCGATGTATTGCTGGTGAGCG                                                       <  SRR4302537.3251692/94‑1 (MQ=60)

                                        CTGTTTATCAACACCGCTGGCATGGGCGCAATTCCGGCGAATATTCACTGGGGTGCACAGACGCTAACCGCAGGCGATGTATTGCTGGTGAGCG                                                       <  SRR4302537.5422128/94‑1 (MQ=60)

                                         TGTTTATCAACACCGCTGGCATGGGCGCAATTCCGGCGAATATTCACTGGGGTGCACAGACGCTAACCGCAGGCGATGTATTGCTGGTGAGCGG                                                      >  SRR4302537.4031903/1‑94 (MQ=60)

                                           TTTATCAACACCGCTGGCATGGGCGCAATTCCGGCGAATATTCACTGGGGTGCACAGACGCTAACCGCAGGCGATGTATTGCTGGTGAGCGGTA                                                    >  SRR4302537.3414053/1‑94 (MQ=60)

                                            TTATCAACACCGCTGGCATGGGCGCAATTCCGGCGAATATTCACTGGGGTGCACAGACGCTAACCGCAGGCGATGTATTGCTGGTGAGCGGTAC                                                   >  SRR4302537.3159402/1‑94 (MQ=60)

                                            TTATCAACACCGCTGGCATGGGCGCAATTCCGGCGAATATTCACTGGGGTGCACAGACGCTAACCGCAGGCGATGTATTGCTGGTGAGCGGTAC                                                   >  SRR4302537.5380503/1‑94 (MQ=60)

                                               TCAACACCGCTGGCATGGGCGCAATTCCGGCGAATATTCACTGGGGTGCACAGACGCTAACCGCAGGCGATGTATTGCTGGTGAGCGGTACACT                                                <  SRR4302537.5089485/94‑1 (MQ=60)

                                               TCAACACCGCTGGCATGGGCGCAATTCCGGCGAATATTCACTGGGGTGCACAGACGCTAACCGCAGGCGATGTATTGCTGGTGAGCGGTACACT                                                >  SRR4302537.2006852/1‑94 (MQ=60)

                                               TCAACACCGCTGGCATGGGCGCAATTCCGGCGAATATTCACTGGGGTGCACAGACGCTAACCGCAGGCGATGTATTGCTGGTGAGCGGTACACT                                                >  SRR4302537.3144624/1‑94 (MQ=60)

                                                CAACACCGCTGGCATGGGCGCAATTCCGGCGAATATTCACTGGGGTGCACAGACGCTAACCGCAGGCGATGTATTGCTGGTGAGCGGTACACTC                                               <  SRR4302537.3446966/94‑1 (MQ=60)

                                                CAACACCGCTGGCATGGGCGCAATTCCGGCGAATATTCACTGGGGTGCACAGACGCTAACCGCAGGCGATGTATTGCTGGTGAGCGGTACACTC                                               <  SRR4302537.5544123/94‑1 (MQ=60)

                                                CAACACCGCTGGCATGGGCGCAATTCCGGCGAATATTCACTGGGGTGCACAGACGCTAACCGCAGGCGATGTATTGCTGGTGAGCGGTACACTC                                               <  SRR4302537.4827390/94‑1 (MQ=60)

                                                 AACACCGCTGGCATGGGCGCAATTCCGGCGAATATTCACTGGGGTGCACAGACGCTAACCGCAGGCGATGTATTGCTGGTGAGCGGTACACTCG                                              <  SRR4302537.6230255/94‑1 (MQ=60)

                                                 AACACCGCTGGCATGGGCGCAATTCCGGCGAATATTCACTGGGGTGCACAGACGCTAACCGCAGGCGATGTATTGCTGGTGAGCGGTACACTCG                                              <  SRR4302537.1570653/94‑1 (MQ=60)

                                                  ACACCGCTGGCATGGGCGCAATTCCGGCGAATATTCACTGGGGCGCACAGACGCTAACCGCAGGCGATGTATTGCTGGTGAGCGGTACACCCGG                                             >  SRR4302537.1294569/1‑94 (MQ=60)

                                                   CACCGCTGGCATGGGCGCAATTCCGGCGAATATTCACTGGGGTGCACAGACGCTAACCGCAGGCGATGTATTGCTGGTGAGCGGTACACTCGGC                                            <  SRR4302537.5342655/94‑1 (MQ=60)

                                                   CACCGCTGGCATGGGCGCAATTCCGGCGAATATTCACTGGGGTGCACAGACGCTAACCGCAGGCGATGTATTGCTGGTGAGCGGTACACTCGGC                                            >  SRR4302537.839429/1‑94 (MQ=60)

                                                    ACCGCTGGCATGGGCGCAATTCCGGCGAATATTCACTGGGGTGCACAGACGCTAACCGCAGGCGATGTATTGCTGGTGAGCGGTACACTCGGCG                                           >  SRR4302537.1610716/1‑94 (MQ=60)

                                                    ACCGCTGGCATGGGCGCAATTCCGGCGAATATTCACTGGGGTGCACAGACGCTAACCGCAGGCGATGTATTGCTGGTGAGCGGTACACTCGGCG                                           <  SRR4302537.3261985/94‑1 (MQ=60)

                                                      CGCTGGCATGGGCGCAATTCCGGCGAATATTCACTGGGGTGCACAGACGCTAACCGCAGGCGATGTATTGCTGGTGAGCGGTACACTCGGCGAC                                         <  SRR4302537.5052076/94‑1 (MQ=60)

                                                       GCTGGCATGGGCGCAATTCCGGCGAATATTCACTGGGGTGCACAGACGCTAACCGCAGGCGATGTATTGCTGGTGAGCGGTACACTCGGCGACC                                        <  SRR4302537.5255656/94‑1 (MQ=60)

                                                       GCTGGCATGGGCGCAATTCCGGCGAATATTCACTGGGGTGCACAGACGCTAACCGCAGGCGATGTATTGCTGGTGAGCGGTACACTCGGCGACC                                        >  SRR4302537.3632261/1‑94 (MQ=60)

                                                        CTGGCATGGGCGCAATTCCGGCGAATATTCACTGGGGTGCACAGACGCTAACCGCAGGCGATGTATTGCTGGTGAGCGGTACACTCGGCGACCA                                       >  SRR4302537.2922778/1‑94 (MQ=60)

                                                        CTGGCATGGGCGCAATTCCGGCGAATATTCACTGGGGTGCACAGACGCTAACCGCAGGCGATGTATTGCTGGTGAGCGGTACACTCGGCGACCA                                       >  SRR4302537.2272548/1‑94 (MQ=60)

                                                        CTGGCATGGGCGCAATTCCGGCGAATATTCACTGGGGCGCACAGACGCTAACCGCAGGCGATGCATTGCTGGTGAGCGGTACACTCGGCGACCA                                       >  SRR4302537.4093804/1‑94 (MQ=60)

                                                         TGGCATGGGCGCAATTCCGGCGAATATTCACTGGGGTGCACAGACGCTAACCGCAGGCGATGTATTGCTGGTGAGCGGTACACTCGGCGACCAC                                      >  SRR4302537.332908/1‑94 (MQ=60)

                                                         TGGCATGGGCGCAATTCCGGCGAATATTCACTGGGGTGCACAGACGCTAACCGCAGGCGATGTATTGCTGGTGAGCGGTACACTCGGCGACCAC                                      >  SRR4302537.2890263/1‑94 (MQ=60)

                                                         TGGCATGGGCGCAATTCCGGCGAATATTCACTGGGGTGCACAGACGCTAACCGCAGGCGATGTATTGCTGGTGAGCGGTACACTCGGCGACCAC                                      <  SRR4302537.835150/94‑1 (MQ=60)

                                                          GGCATGGGCGCAATTCCGGCGAATATTCACTGGGGTGCACAGACGCTAACCGCAGGCGATGTATTGCTGGTGAGCGGTACACTCGGCGACCACG                                     <  SRR4302537.1750069/94‑1 (MQ=60)

                                                           GCATGGGCGCAATTCCGGCGAATATTCACTGGGGTGCACAGACGCTAACCGCAGGCGATGTATTGCTGGTGAGCGGTACACTCGGCGACCACGG                                    <  SRR4302537.851562/94‑1 (MQ=60)

                                                            GATGGGCGCAATTCCGGCGAATATTCACTGGGGTGCACAGACGCTAACCGCAGGCGATGTATTGCTGGTGAGCGGTACACTCGGCGTCCACGGG                                   <  SRR4302537.3324354/94‑1 (MQ=60)

                                                            CATGGGCGCAATTCCGGCGAATATTCACTGGGGTGCACAGACGCTAACCGCAGGCGATGTATTGCTGGTGAGCGGTACACTCGGCGACCACGGG                                   <  SRR4302537.6080735/94‑1 (MQ=60)

                                                            CATGGGCGCAATTCCGGCGAATATTCACTGGGGTGCACAGACGCTAACCGCAGGCGATGTATTGCTGGTGAGCGGTACACTCGGCGACCACGGG                                   <  SRR4302537.106000/94‑1 (MQ=60)

                                                             ATGGGCGCAATTCCGGCGAATATTCACTGGGGTGCACAGACGCTAACCGCAGGCGATGTATTGCTGGTGAGCGGTACACTCGGCGACCACGGGG                                  >  SRR4302537.505109/1‑94 (MQ=60)

                                                              TGGGCGCAATTCCGGCGAATATTCACTGGGGTGCACAGACGCTAACCGCAGGCGATGTATTGCTGGTGAGCGGTACACTCGGCGACCACGGGGC                                 <  SRR4302537.6040156/94‑1 (MQ=60)

                                                                GGCGCAATTCCGGCGAATATTCACTGGGGTGCACAGACGCTAACCGCAGGCGATGTATTGCTGGTGAGCGGTACACTCGGCGACCACGGGGCGA                               >  SRR4302537.2379353/1‑94 (MQ=60)

                                                                 GCGCAATTCCGGCGAATATTCACTGGGGTGCACAGACGCTAACCGCAGGCGATGTATTGCTGGTGAGCGGTACACTCGGCGACCACGGGGCGAC                              >  SRR4302537.4416318/1‑94 (MQ=60)

                                                                   GCAATTCCGGCGAATATTCACTGGGGTGCACAGACGCTAACCGCAGGCGATGTATTGCTGGTGAGCGGTACACTCGGCGACCACGGGGCGACTA                            >  SRR4302537.502044/1‑94 (MQ=60)

                                                                   GCAATTCCGGCGAATATTCACTGGGGCGCACAGACGCTAACCGCAGGCGATGTATTGCTGGTGAGCGGTACACTCGGCGACCACGGGGCGACTA                            >  SRR4302537.1736966/1‑94 (MQ=60)

                                                                    CAATTCCGGCGAATATTCACTGGGGTGCACAGACGCTAACCGCAGGCGATGTATTGCTGGTGAGCGGTACACTCGGCGACCACGGGGCGACTAT                           <  SRR4302537.2723794/94‑1 (MQ=60)

                                                                      ATTCCGGCGAATATTCACTGGGGTGCACAGACGCTAACCGCAGGCGATGTATTGCTGGTGAGCGGTACACTCGGCGACCACGGGGCGACTATCC                         <  SRR4302537.2798609/94‑1 (MQ=60)

                                                                       TTCCGGCGAATATTGACTGGTGTGCACAGACTCTAACCGCTGGCGATGTATTGCTGGTGAGCGGTACGCTCGGCGACCACGGGGCGACTATCCT                        <  SRR4302537.512171/94‑1 (MQ=60)

                                                                         CCGGCGAATATTCACTGGGGTGCACGGACGCTAGCCGCAGGCGATGTATTGCTGGTGAGCGGTACACTCGGCGACCACGGGGCGACTATCCTTA                      <  SRR4302537.6467043/94‑1 (MQ=60)

                                                                         CCGGCGAATATTCACTGGGGTGCACAGACGCTAACCGCAGGCGATGTATTGCTGGTGAGCGGTACACTCGGCGACCACGGGGCGACTATCCTTA                      >  SRR4302537.1065358/1‑94 (MQ=60)

                                                                          CGGCGAATATTCACTGGGGTGCACAGACGCTAACCGCAGGCGATGTATTGCTGGTGAGCGGTACACTCGGCGACCACGGGGCGACTATCCTTAA                     <  SRR4302537.3465402/94‑1 (MQ=60)

                                                                          CGGCGAATATTCACTGGGGTGCACAGACGCTAACCGCAGGCGATGTATTGCTGGTGAGCGGTACACTCGGCGACCACGGGGCGACTATCCTTAA                     <  SRR4302537.5511264/94‑1 (MQ=60)

                                                                           GGCGAATATTCACTGGGGTGCACAGACGCTAACCGCAGGCGATGTATTGCTGGTGAGCGGTACACTCGGCGACCACGGGGCGACTATCCTTAAC                    <  SRR4302537.3224790/94‑1 (MQ=60)

                                                                           GGCGAATATTCACTGGGGTGCACAGACGCTAACCGCAGGCGATGTATTGCTGGTGAGCGGTACACTCGGCGACCACGGGGCGACTATCCTTAAC                    <  SRR4302537.1401168/94‑1 (MQ=60)

                                                                            GCGAATATTCACTGGGGTGCACAGACGCTAACCGCAGGCGATGTATTGCTGGTGAGCGGTACACTCGGCGACCACGGGGCGACTATCCTTAACC                   >  SRR4302537.3518843/1‑94 (MQ=60)

                                                                             CGAATATTCACTGGTGTGCACAGACGCTAACCGCAGGCGATGTATTGCTGGTGAGCGGTACACTCGGCGACCACGGGGCGACTATCCTTAACCT                  >  SRR4302537.5015293/1‑94 (MQ=60)

                                                                             CGAATATTCACTGGGGTGCACAGACGCTAACCGCAGGCGATGTATTGCTGGTGAGCGGTACACTCGGCGACCACGGGGCGACTATCCTTAACCT                  >  SRR4302537.2441431/1‑94 (MQ=60)

                                                                             CGAATATTCACTGGGGTGCACAGACGCTAACCGCAGGCGATGTATTGCTGGTGAGCGGTACACTCGGCGACCACGGGGCGACTATCCTTAACCT                  <  SRR4302537.6437114/94‑1 (MQ=60)

                                                                              GAATATTCACTGGGGTGCACAGACGCTAACCGCAGGCGATGTATTGCTGGTGAGCGGTACACTCGGCGACCACGGGGCGACTATCCTTAACCTG                 >  SRR4302537.759587/1‑94 (MQ=60)

                                                                               AATATTCACTGGGGTGCACAGACGCTAACCGCAGGCGATGTATTGCTGGTGAGCGGTACACTCGGCGACCACGGGGCGACTATCCTTAACCTGC                <  SRR4302537.5362867/94‑1 (MQ=60)

                                                                                ATATTCACTGGGGTGCACAGACGCTAACCGCAGGCGATGTATTGCTGGTGAGCGGTACACTCGGCGACCACGGGGCGACTATCCTTAACCTGCG               >  SRR4302537.1690902/1‑94 (MQ=60)

                                                                                ATATTCACTGGGGTGCACAGACGCTAACCGCAGGCGATGTATTGCTGGTGAGCGGTACACTCGGCGACCACGGGGCGACTATCCTTAACCTGCG               <  SRR4302537.426483/94‑1 (MQ=60)

                                                                                ATATTCACTGGGGTGCACAGACGCTAACCGCAGGCGATGTATTGCTGGTGAGCGGTACACTCGGCGACCACGGGGCGACTATCCTTAACCTGCG               >  SRR4302537.4085148/1‑94 (MQ=60)

                                                                                ATATTCACTGGGGTGCACAGACGCTAACCGCAGGCGATGTATTGCTGGTGAGCGGTACACTCGGCGACCACGGGGCGACTATCCTTAACCTGCG               <  SRR4302537.1445181/94‑1 (MQ=60)

                                                                                  ATTCACTGGGGTGCACAGACGCTAACCGCAGGCGATGTATTGCTGGTGAGCGGTACACTCGGCGACCACGGGGCGACTATCCTTAACCTGCGTG             >  SRR4302537.2199602/1‑94 (MQ=60)

                                                                                  ATTCACTGGGGTGCACAGACGCTAACCGCAGGCGATGTATTGCTGGTGAGCGGTACACTCGGCGACCACGGGGCGACTATCCTTAACCTGCGTG             >  SRR4302537.6406813/1‑94 (MQ=60)

                                                                                  ATTCACTGGGGTGCACAGACGCTAACCGCAGGCGATGTATTGCTGGTGAGCGGTACACTCGGCGACCACGGGGCGACTATCCTTAACCTGCGTG             >  SRR4302537.2268692/1‑94 (MQ=60)

                                                                                  ATTCACTGGGGTGCACAGACGCTAACCGCAGGCGATGTATTGCTGGTGAGCGGTACACTCGGCGACCACGGGGCGACTATCCTTAACCTGCGTG             >  SRR4302537.1410001/1‑94 (MQ=60)

                                                                                   TTCACTGGGGTGCACAGACGCTAACCGCAGGCGATGTATTGCTGGTGAGCGGTACACTCGGCGACCACGGGGCGACTATCCTTAACCTGCGTGA            <  SRR4302537.2845760/94‑1 (MQ=60)

                                                                                   TTCACTGGGGTGCACAGACGCTAACCGCAGGCGATGTATTGCTGGTGAGCGGTACACTCGGCGACCACGGGGCGACTATCCTTAACCTGCGTGA            >  SRR4302537.4614947/1‑94 (MQ=60)

                                                                                     CACTGGGGTGCACAGACGCTAACCGCAGGCGATGTATTGCTGGTGAGCGGTACACTCGGCGACCACGGGGCGACTATCCTTAACCTGCGTGAGC          >  SRR4302537.2807469/1‑94 (MQ=60)

                                                                                       CTGGGGTGCACAGACGCTAACCGCAGGCGATGTATTGCTGGTGAGCGGTACACTCGGCGACCACGGGGCGACTATCCTTAACCTGCGTGAGCAG        >  SRR4302537.65012/1‑94 (MQ=60)

                                                                                       CTGGGGTGCACAGACGCTAACCGCAGGCGATGTATTGCTGGTGAGCGGTACACTCGGCGACCACGGGGCGACTATCCTTAACCTGCGTGAGCAG        >  SRR4302537.924875/1‑94 (MQ=60)

                                                                                        TGGGGTGCACAGACGCTAACCGCAGGCGATGTATTGCTGGTGAGCGGTACACTCGGCGACCACGGGGCGACTATCCTTCACCTGCGTGAGCAGC       >  SRR4302537.4245573/1‑94 (MQ=60)

                                                                                        TGGGGTGCACAGACGCTAACCGCAGGCGATGTATTGCTGGTGAGCGGTACACTCGGCGACCACGGGGCGACTATCCTTAACCTGCGTGAGCAGC       <  SRR4302537.1299946/94‑1 (MQ=60)

                                                                                         GGGGTGCACAGACGCTAACCGCAGGCGATGTATTGCTGGTGAGCGGTACACTCGGCGACCACGGGGCGACTATCCTTAACCTGCGTGAGCAGCT      >  SRR4302537.751070/1‑94 (MQ=60)

                                                                                           GGTGCACAGACGCTAACCGCAGGCGATGTATTGCTGGTGAGCGGTACACTCGGCGACCACGGGGCGACTATCCTTAACCTGCGTGAGCAGCTGG    <  SRR4302537.3380620/94‑1 (MQ=60)

                                                                                             TGCACAGACGCTAACCGCAGGCGATGTATTGCTGGTGAGCGGTACACTCGGCGACCACGGGGCGACTATCCTTAACCTGCGTGAGCAGCTGGGG  <  SRR4302537.4554310/94‑1 (MQ=60)

TGGCGATACTAAAGTGGTGCAGCGCGGCGCGGTAGATAAACTGTTTATCAACACCGCTGGCATGGGCGCAATTCCGGCGAATATTCACTGGGGCGCACAGACGCTAACCGCAGGCGATGTATTGCTGGTGAGCGGTACACTCGGCGACCACGGGGCGACTATCCTTAACCTGCGTGAGCAGCTGGGG  >  NC_000913/2851663‑2851849

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 0 ≤` `ATCG/ATCG` `< 2 ≤` `ATCG/ATCG` `< 26 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`