Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I2 R1 882 254.4 12957588 98.0% 12698436 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,863,578 T→C L152P (CTC→CCC)  b2740 → putative transport protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,863,5780TC94.3% 705.2 / 24.5 228L152P (CTC→CCC) b2740putative transport protein
Reads supporting (aligned to +/- strand):  ref base T (8/5);  new base C (133/82);  total (141/87)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.59e-01

Only 100 of 230 total aligned reads displayed.

TTATTATTCTTGCGCCAATCATTTACGGTTTTGCCAAGGTTGCCAAAATATCGCCACTCAAATTTGGCCTGCCTGTCGCTGGGATCATGCTCACTGTTCACGTGGCGGTACCGCCGCATCCAGGCCCTGTCGCCGCAGCGGGGTTACTCCACGCAGACATCGGCTGGCTAACCATCATCG  >  NC_000913/2863488‑2863667
                                                                                          |                                                                                         
ttattaTTCTTGCGCCAATCATTTACGGTTTTGCCAAGGTTGCCAAAATATCGCCACTCAAATTTGGCCTGCCTGTCGCTGGGATCATGCCCa                                                                                         >  2:3928089/1‑93 (MQ=255)
ttattaTTCTTGCGCCAATCATTTACGGTTTTGCCAAGGTTGCCAAAATATCGCCACTCAAATTTGGCCTGCCTGTCGCTGGGATCATGCCCa                                                                                         >  2:339555/1‑93 (MQ=255)
ttattaTTCTTGCGCCAATCATTTACGGTTTTGCCAAGGTTGCCAAAATATCGCCACTCAAATTTGGCCTGCCTGTCGCTGGGATCATGCCCa                                                                                         >  2:2365442/1‑93 (MQ=255)
 tattatTCTTGCGCCAATCATTTACGGTTTTGCCAAGGTTGCCAAAATATCGCCACTCAAATTTGGCCTGCCTGTCGCTGGGATCATGCCCAc                                                                                        >  2:806317/1‑93 (MQ=255)
 tattatTCTTGCGCCAATCATTTACGGTTTTGCCAAGGTTGCCAAAATATCGCCACTCAAATTTGGCCTGCCTGTCGCTCGGATCATGCCCAc                                                                                        >  2:544214/1‑93 (MQ=255)
  attattCTTGCGCCAATCATTTACGGTTTTGCCAAGGTTGCCAAAATATCGCCACTCAAATTTGGCCTGCCTGTCGCTGGGATCATGCCCACt                                                                                       <  1:132680/93‑1 (MQ=255)
  attattCTTGCGCCAATCATTTACGGTTTTGCCAAGGTTGCCAAAATATCGCCACTCAAATTTGGCCTGCCTGTCGCTGGGATCATGCCCACt                                                                                       >  2:1931955/1‑93 (MQ=255)
  attattCTTGCGCCAATCATTTACGGTTTTGCCAAGGTTGCCAAAATATCGCCACTCAAATTTGGCCTGCCTGTCGCTGGGATCATGCCCACt                                                                                       >  2:2434397/1‑93 (MQ=255)
    tattCTTGCGCCAATCATTTACGGTTTTGCCAAGGTTGCCAAAATATCGCCACTCAAATTTGGCCTGCCTGTCGCTGGGATCATGCCCACTGt                                                                                     >  2:2205573/1‑93 (MQ=255)
      ttCTGGCGCCAATCATTTACGGTTTTGCCAAGGTTGCCAAAATATCGCCACTCAAATTTGGCCTGCCTGTCGCTGGGATCATGCCCACTGTTc                                                                                   >  2:5036771/1‑93 (MQ=255)
        cTTGCGCCAATCATTTACGGTTTTGCCAAGGTTGCCAAAATATCGCCACTCAAATTTGGCCTGCCTGTCGCTGGGATCATGCCCACTGTTCAc                                                                                 <  1:3822932/93‑1 (MQ=255)
         ttGCGCCAATCATTTACGGTTTTGCCAAGGTTGCCAAAATATCGCCACTCAAATTTGGCCTGCCTGTCGCTGGGATCATGCTCACTGTTCACg                                                                                >  2:4399023/1‑93 (MQ=255)
         ttGCGCCAATCATTTACGGTTTTGCCAAGGTTGCCAAAATATCGCCACTCAAATTTGGCCTGCCTGTCGCTGGGATCATGCCCACTGTTCACg                                                                                >  2:5469947/1‑93 (MQ=255)
           gcggCAGTCATTTACGGTTTTGCCAAGGTTGCCAAAATATCGCCACTCAAATTTGGCCTGCCTGTCGCTGGGATCATGCCCACTGTTCACGTg                                                                              <  2:887990/93‑1 (MQ=255)
             gcCAATCATTTACGGTTTTGCCAAGGTTGCCAAAATATCGCCACTCAAATTTGGCCTGCCTGTCGCTGGGATCATGCCCACTGTTCACGTGGc                                                                            <  1:10355/93‑1 (MQ=255)
             gcCAATCATTTACGGTTTTGCCAAGGTTGCCAAAATATCGCCACTCAAATTTGGCCTGCCTGTCGCTGGGATCATGCCCACTGTTCACGTGGc                                                                            >  1:6084754/1‑93 (MQ=255)
              ccAATCATTTACGGTTTTGCCAAGGTTGCCAAAATATCGCCACTCAAATTTGGCCTGCCTGTCGCTGGGATCATGCCCACTGTTCACGTGGCg                                                                           >  1:3838462/1‑93 (MQ=255)
              ccAATCATTTACGGTTTTGCCAAGGTTGCCAAAATATCGCCACTCAAATTTGGCCTGCCTGTCGCTGGGATCATGCCCACTGTTCACGTGGCg                                                                           <  1:3133007/93‑1 (MQ=255)
                aaTCATTTACGGTTTTGCCAAGGTTGCCAAAATATCGCCACTCAAATTTGGCCTGCCTGTCGCTGGGATCATGCTCACTGTTCACGTGGCGGt                                                                         >  2:373979/1‑93 (MQ=255)
                aaTCATTTACGGTTTTGCCAAGGTTGCCAAAATATCGCCACTCAAATTTGGCCTGCCTGTCGCTGGGATCATGCCCACTGTTCACGTGGCGGt                                                                         >  1:4973736/1‑93 (MQ=255)
                aaTCATTTACGGTTTTGCCAAGGTTGCCAAAATATCGCCACTCAAATTTGGCCTGCCTGTCGCTGGGATCATGACCACTGTTCACGCGCCgga                                                                         >  1:5057029/1‑92 (MQ=255)
                 aTCATTTACGGTTTTGCCAAGGTTGCCAAAATATCGCCACTCAAATTTGGCCTGCCTGTCGCTGGGATCATGCCCACTGTTCACGTGGCGGTa                                                                        <  1:82821/93‑1 (MQ=255)
                 aTCATTTACGGTTTTGCCAAGGTTGCCAAAATATCGCCACTCAAATTTGGCCTGCCTGTCGCTGGGATCATGCCCACTGTTCACGTGGCGGTa                                                                        >  1:5183028/1‑93 (MQ=255)
                 aTCATTTACGGTTTTGCCAAGGTTGCCAAAATATCGCCACTCAAATTTGGCCTGCCTGTCGCTGGGATCATGCCCACTGTTCACGTGGCGGTa                                                                        >  2:3268808/1‑93 (MQ=255)
                 aTCATTTACGGTTTTGCCAAGGTTGCCAAAATATCGCCACTCAAATTTGGCCTGCCTGTCGCTGGGATCATGCCCACTGTTCACGTGGCGGTa                                                                        >  1:2281509/1‑93 (MQ=255)
                  tCATTTACGGTTTTGCCAAGGTTGCCAAAATATCGCCACTCAAATTTGGCCTGCCTGTCGCTGGGATCATGCCCACTGTTCACGTGGCGGTAc                                                                       <  1:1155634/93‑1 (MQ=255)
                   cATTTACGGTTTTGCCAAGGTTGCCAAAATATCGCCACTCAAATTTGGCCTGCCTGTCGCTGGGATCATGCTCACTGTTCACGTGGCGGTAcc                                                                      >  1:5365298/1‑93 (MQ=255)
                   cATTTACGGTTTTGCCAAGGTTGCCAAAATATCGCCACTCAAATTTGGCCTGCCTGTCGCTGGGATCATGCCCACTGTTCACGTGGCGGTAcc                                                                      >  1:4038664/1‑93 (MQ=255)
                   cATTTACGGTTTTGCCAAGGTTGCCAAAATATCGCCACTCAAATTTGGCCTGCCTGTCGCTGGGATCATGCCCACTGTTCACGTGGCGGTAcc                                                                      >  1:4454869/1‑93 (MQ=255)
                    aTTTACGGTTTTGCCAAGGTTGCCAAAATATCGCCACTCAAATTTGGCCTGCCTGTCGCTGGGATCATGCCCACTGTTCACGTGGCGGTAccg                                                                     >  1:2254634/1‑93 (MQ=255)
                    aTTTACGGTTTTGCCAAGGTTGCCAAAATATCGCCACTCAAATTTGGCCTGCCTGTCGCTGGGATCATGCCCACTGTTCACGTGGCGGTAccg                                                                     >  2:1758526/1‑93 (MQ=255)
                    aTTTACGGTTTTGCCAAGGTTGCCAAAATATCGCCACTCAAATTTGGCCTGCCTGTCGCTGGGATCATGCCCACTGTTCACGTGGCGGTAccg                                                                     >  1:5431822/1‑93 (MQ=255)
                     tttACGGTTTTGCCAAGGTTGCCAAAATATCGCCACTCAAATTTGGCCTGCCTGTCGCTTGGATCATGCCCACTGTTCACGTTGCGGTAccgc                                                                    >  2:2544391/1‑93 (MQ=255)
                     tttACGGTTTTGCCAAGGTTGCCAAAATATCGCCACTCAAATTTGGCCTGCCTGTCGCTGGGATCATGCCCACTGTTCACGTGGCGGTAccgc                                                                    >  1:3315580/1‑93 (MQ=255)
                      ttACGGTTTTGCCAAGGTTGCCAAAATATCGCCACTCAAATTTGGCCTGCCTGTCGCTGGGATCATGCCCACTGTTCACGTGGCGGTAccgcc                                                                   >  1:577665/1‑93 (MQ=255)
                        aCGGTTTTGCCAAGGTTGCCAAAATATCGCCACTCAAATTTGGCCTGCCTGTCGCTGGGATCATGCCCACTGTTCACGTGGCGGTACcgccgc                                                                 <  2:3917588/93‑1 (MQ=255)
                          ggTTTTGCCAAGGTTGCCAAAATATCGCCACTCAAATTTGGCCTGCCTGTCGCTGGGATCATGCCCACTGTTCACGTGGCGGTACCGCCGCAt                                                               >  2:5370049/1‑93 (MQ=255)
                          ggTTTTGCCAAGGTTGCCAAAATATCGCCACTCAAATTTGGCCTGCCTGTCGCTGGGATCATGCCCACTGTTCACGTGGCGGTACCGCCGCAt                                                               <  2:4772195/93‑1 (MQ=255)
                           gTTTTGCCAAGGTTGCCAAAATATCGCCACTCAAATTTGGCCTGCCTGTCGCTGGGATCATGCCCACTGTTCACGTGGCGGTACCGCCGCATc                                                              >  1:4509067/1‑93 (MQ=255)
                           gTTTTGCCAAGGTTGCCAAAATATCGCCACTCAAATTTGGCCTGCCTGTCGCTGGGATCATGCCCACTGTTCACGTGGCGGTACCGCCGCATc                                                              >  1:878105/1‑93 (MQ=255)
                            ttttGCCAAGGTTGCCAAAATATCGCCACTCAAATTTGGCCTGCCTGTCGCTGGGATCATGCCCACTGTTCACGTGGCGGTACCGCCGCATcc                                                             <  1:3736310/93‑1 (MQ=255)
                             tttGCCAAGGTTGCCAAAATATCGCCACTCAAATTTGGCCTGCCTGTCGCTGGGATCATGCCCACTGTTCACGTGGCGGTACCGCCGCATCCa                                                            <  1:2335747/93‑1 (MQ=255)
                             tttGCCAAGGTTGCCAAAATATCGCCACTCAAATTTGGCCTGCCTGTCGCTGGGATCATGCCCACTGTTCACGTGGCGGTACCGCCGCATCCa                                                            <  2:1129429/93‑1 (MQ=255)
                                 ccAAGGTTGCCAAAATATCGCCACTCAAATTTGGCCTGCCTGTCGCTGGGATCATGCCCACTGTTCACGTGGCGGTACCGCCGCATCCAGGcc                                                        >  2:6249770/1‑93 (MQ=255)
                                  cAAGGTTGCCAAAATATCGCCACTCAAATTTGGCCTGCCTGTCGCTGGGATCATGCCCACTGTTCACGTGGCGGTACCGCCGCATCCAGGccc                                                       <  1:5943095/93‑1 (MQ=255)
                                  cAAGGGTGCCAAAATATCGCCACTCAAATTTGGCCTGCCTGTCGCTGGGATCATGCCCACTGTTCACGTGGCGGTACCGCCGCATCCAGGccc                                                       >  2:2646478/1‑93 (MQ=255)
                                    aGGTTGCCAAAATATCGCCACTCAAATTTGGCCTGCCTGTCGCTGGGATCATGCCCACTGTTCACGTGGCGGTACCGCCGCATCCAGGCCCTg                                                     >  1:179365/1‑93 (MQ=255)
                                    aGGTTGCCAAAATATCGCCACTCAAATTTGGCCTGCCTGTCGCTGGGATCATGCCCACTGTTCACGTGGCGGTACCGCCGCATCCAGGCCCTg                                                     >  2:4656947/1‑93 (MQ=255)
                                     ggTTGCCAAAATATCGCCACTCAAATTTGGCCTGCCTGTCGCTGGGATCATGCCCACTGTTCACGTGGCGGTACCGCCGCATCCAGGCCCTGt                                                    <  2:3402243/93‑1 (MQ=255)
                                     ggTTGCCAAAATATCGCCACTCAAATTTGGCCTGCCTGTCGCTGGGATCATGCCCACTGTTCACGTGGCGGTACCGCCGCATCCAGGCCCTGt                                                    <  1:54654/93‑1 (MQ=255)
                                      gTTGCCAAAATATCGCCACTCAAATTTGGCCTGCCTGTCGCTGGGATCATGCCCACTGTTCACGTGGCGGTACCGCCGCATCCAGGCCCTGTc                                                   >  2:5558994/1‑93 (MQ=255)
                                      gTTGCCAAAATATCGCCACTCAAATTTGGCCTGCCTGTCGCTGGGATCATGCCCACTGTTCACGTGGCGGTACCGCCGCATCCAGGCCCTGTc                                                   >  2:6226089/1‑93 (MQ=255)
                                        tGCCAAAATATCGCCACTCAAATTTGGCCTGCCTGTCGCTGGGATCATGCCCACTGTTCACGTGGCGGTACCGCCGCATCCAGGCCCTGTcgc                                                 <  1:6001326/93‑1 (MQ=255)
                                         gCCAAAATATCGCCACTCAAATTTGGCCTGCCTGTCGCTGGGATCATGCCCACTGTTCACGTGGCGGTACCGCCGCATCCAGGCCCTGTcgcc                                                >  1:3641011/1‑93 (MQ=255)
                                         gCCAAAATATCGCCACTCAAATTTGGCCTGCCTGTCGCTGGGATCATGCCCACTGTTCACGTGGCGGTACCGCCGCATCCAGGCCCTGTcgcc                                                >  2:5302178/1‑93 (MQ=255)
                                          ccAAAATATCGCCACTCAAATTTGGCCTGCCTGTCGCTGGGATCATGCCCACTGTTCACGTGGCGGTACCGCCGCATCCAGGCCCTGTcgccg                                               <  2:5829407/93‑1 (MQ=255)
                                          ccAAAATATCGCCACTCAAATTTGGCCTGCCTGTCGCTGGGATCATGCCCACTGTTCACGTGGCGGTACCGCCGCATCCAGGCCCTGTcgccg                                               >  1:5859482/1‑93 (MQ=255)
                                            aaaaTATCGCCACTCAAATTTGGCCTGCCTGTCGCTGGGATCATGCCCACTGTTCACGTGGCGGTACCGCCGCATCCAGGCCCTGTCGCCGCa                                             <  2:369710/93‑1 (MQ=255)
                                              aaTATCGCCGCTCAAATTTGGCCTGCCTGTCGCTGGGATCATGCCCACTGTTCACGTGGCGGTACCGCCGCATCCAGGCCCTGTCGCCGCAGc                                           <  1:432277/93‑1 (MQ=255)
                                              aaTATCGCCACTCAAATTTGGCCTGCCTGTCGCTGGGATCATGCCCACTGTTCACGTGGCGGTACCGCCGCATCCAGGCCCTGTcgccgccac                                           >  2:3690947/1‑90 (MQ=255)
                                              aaTATCGCCACTCAAATTTGGCCTGCCTGTCGCTGGGATCATGCCCACTGTTCACGTGGCGGTACCGCCGCATCCAGGCCCTGTCGCCGCAGc                                           <  2:2077173/93‑1 (MQ=255)
                                                tatCGCCACTCAAATTTGGCCTGCCTGTCGCTGGGATCATGCCCACTGTTCACGTGGCGGTACCGCCGCATCCAGGCCCTGTCGCCGCAGCgg                                         <  2:1162988/93‑1 (MQ=255)
                                                tatCGCCACTCAAATTTGGCCTGCCTGTCGCTGGGATCATGCCCACTGTTCACGTGGCGGTACCGCCGCATCCAGGCCCTGTCGCCGCAGCgg                                         <  1:3957555/93‑1 (MQ=255)
                                                    gCCACTCAAATTTGGCCTGCCTGTCGCTGGGATCATGCCCACTGTTCACGTGGCGGTACCGCCGCATCCAGGCCCTGTCGCCGCAGCGGGGtt                                     <  1:1398562/93‑1 (MQ=255)
                                                     ccACTCAAATTTGGCCTGCCTGTCGCTGGGATCATGCCCACTGTTCACGTGGCGGTACCGCCGCATCCAGGCCCTGTCGCCGCAGCGGGGTTa                                    <  2:5020174/93‑1 (MQ=255)
                                                      cACTCAAATTTGGCCTGCCTGTCGCTGGGATCATGCCCACTGTTCACGTGGCGGTACCGCCGCATCCAGGCCCTGTCGCCGCAGCGGGGTTAc                                   >  2:6164277/1‑93 (MQ=255)
                                                      cACTCAAATTTGGCCTGCCTGTCGCTGGGATCATGCCCACTGTTCACGTGGCGGTACCGCCGCATCCAGGCCCTGTCGCCGCAGCGGGGTTAc                                   >  2:5133250/1‑93 (MQ=255)
                                                      cACTCAAATTTGGCCTGCCTGTCGCTGGGATCATGCCCACTGTTCACGTGGCGGTACCGCCGCATCCAGGCCCTGTCGCCGCAGCGGGGTTAc                                   >  1:4073346/1‑93 (MQ=255)
                                                      cACTCAAATTTGGCCTGCCTGTCGCTGGGATCATGCCCACTGTTCACGTGGCGGTACCGCCGCATCCAGGCCCTGTCGCCGCAGCGGGGTTAc                                   <  1:5727573/93‑1 (MQ=255)
                                                        cTCAAATTTGGCCTGCCTGTCGCTGGGATCATGCTCACTGTTCACGTGGCGGTACCGCCGCATCCAGACCCTGTCGCCGCAGCGGGGTTACTc                                 >  1:6248685/1‑93 (MQ=255)
                                                            aaTTTGGCCTGCCTGTCGCTGGGATCATGCCCACTGTTCACGTGGCGGTACCGCCGCATCCAGGCCCTGTCGCCGCAGCGGGGTTACTCCACg                             >  1:3154532/1‑93 (MQ=255)
                                                            aaTTTGGCCTGCCTGTCGCTGGGATCATGCCCACTGTTCACGTGGCGGTACCGCCGCATCCAGGCCCTGTCGCCGCAGCGGGGTTACTCCACg                             >  2:4480374/1‑93 (MQ=255)
                                                            aaTTTGGCCTGCCTGTCGCTGGGATCATGCCCACTGTTCACGTGGCGGTACCGCCGCATCCAGGCCCTGTCGCCGCAGCGGGGTTACTCCACg                             >  2:4153581/1‑93 (MQ=255)
                                                            aaTTTGGCCTGCCTGTCGCTGGGATCATGCCCACTGTTCACGTGGCGGTACCGCCGCATCCAGGCCCTGTCGCCGCAGCGGGGTTACTCCACg                             >  2:1028704/1‑93 (MQ=255)
                                                              tttGGCCTGCCTGTCGCTGGGATCATGCCCACTGTTCACGTGGCGGTACCGCCGCATCCAGGCCCTGTCGCCGCAGCGGGGTTACTCCACGCa                           >  2:3451944/1‑93 (MQ=255)
                                                               ttGGCCTGCCTGTCGCTGGGATCATGCCCACTGTTCACGTGGCGGTACCGCCGCATCCAGGCCCTGTCGCCGCAGCGGGGTTACTCCACGCAg                          >  2:1254140/1‑93 (MQ=255)
                                                                tgtcctgcctgTCGCTGGGATCATGCTCACTGTTCACGTGGCGGTACCGCCGCATCCAGGCCCTGTCGCCGCAGCGGGGTTACTCCACGCAGa                         <  2:2971859/90‑1 (MQ=255)
                                                                  gcctgcctGTCGCTGGGATCATGCCCACTGTTCACGTGGCGGTACCGCCGCATCCAGGCCCTGTCGCCGCAGCGGGGTTACTCCACGCAGACa                       <  2:3156194/93‑1 (MQ=255)
                                                                   cctgcctgTCGCTGGGATCATGCCCACTGTTCACGTGGCGGTACCGCCGCATCCAGGCCCTGTCGCCGCAGCGGGGTTACTCCACGCAGACAt                      <  1:3565128/93‑1 (MQ=255)
                                                                   cctgcctgTCGCTGGGATCATGCCCACTGTTCACGTGGCGGTACCGCCGCATCCAGGCCCTGTCGCCGCAGCGGGGTTACTCCACGCAGACAt                      <  1:4841505/93‑1 (MQ=255)
                                                                   cctgcctgTCGCTGGGATCATGCCCACTGTTCACGTGCCGGTACCGCCGCATCCAGGCCCTGTCGCCGCAGCGGGGTTACTCCACGCAGACAt                      <  2:2789846/93‑1 (MQ=255)
                                                                   cctgcctgTCGCTGGGATCATGCCCACTGTTCACGCGGCGGTACCGCCGCATCCAGGCCCTGTCGCCGCAGCGGGGCTACTCCACGCAGACAt                      >  2:3202234/1‑93 (MQ=255)
                                                                    ctgcctgTCGCTGGGATCATGCCCACTGTTCACGTGGCGGTACCGCCGCATCCAGGCCCTGTCGCCGCAGCGGGGTTACTCCACGCAGACATc                     >  1:348170/1‑93 (MQ=255)
                                                                    ctgcctgTCGCTGGGATCATGCCCACTGTTCACGTGGCGGTACCGCCGCATCCAGGCCCTGTCGCCGCAGCGGGGGTACTCCACGCAGACATc                     >  1:709805/1‑93 (MQ=255)
                                                                     tgcctgTCGCTGGGATCATGCTCACTGTTCACGTGGCGGTACCGCCGCATCCAGGCCCTGTCGCCGCAGCGGGGTTACTCCACGCAGACATCg                    <  2:5508531/93‑1 (MQ=255)
                                                                      gcctgTCGCTGGGATCATGCCCACTGTTCACGTGGCGGTACCGCCGCATCCAGGCCCTGTCGCCGCAGCGGGGTTACTCCACGCAGACATCgg                   <  1:666385/93‑1 (MQ=255)
                                                                           tCGCTGGGATCATGCCCACTGTTCACGTGGCGGTACCGCCGCATCCAGGCCCTGTCGCCGCAGCGGGGTTACTCCACGCAGACATCggctggc              <  1:5270225/93‑1 (MQ=255)
                                                                            cGCTGGGATCATGCCCACTGTTCACGTGGCGGTACCGCCGCATCCAGGCCCTGTCGCCGCAGCGGGGTTACTCCACGCAGACATCggctggct             >  1:5961502/1‑93 (MQ=255)
                                                                              cTGGGATCATGCCCACTGTTCACGTGGCGGTACCGCCGCATCCAGGCCCTGTCGCCGCAGCGGGGTTACTCCACGCAGACATCGGCTGGCTaa           >  1:299725/1‑93 (MQ=255)
                                                                                gggATCATGCCCACTGTTCACGTGGCGGTACCGCCGCATCCAGGCCCTGTCGCCGCAGCGGGGTTACTCCACGCAGACATCGGCTGGCTAAcc         >  1:3443784/1‑93 (MQ=255)
                                                                                gggATCATGCCCACTGTTCACGTGGCGGTACCGCCGCATCCAGGCCCTGTCGCCGCAGCGGGGTTACTCCACGCAGACATCGGCTGGCTAAcc         >  1:900152/1‑93 (MQ=255)
                                                                                 ggATCATGCCCACTGTTCACGTGGCGGTACCGCCGCATCCAGGCCCTGTCGCCGCAGCGGGGTTACTCCACGCAGACATCGGCTGGCTAACca        <  1:5619390/93‑1 (MQ=255)
                                                                                  gATCATGCCCACTGTTCACGTGGCGGTACCGCCGCATCCAGGCCCTGTCGCCGCAGCGGGGTTACTCCACGCAGACATCGGCTGGCTAACcat       >  1:3937473/1‑93 (MQ=255)
                                                                                  gATCATGCCCACTGTTCACGTGGCGGTACCGCCGCATCCAGGCCCTGTCGCCGCAGCGGGGTTACTCCACGCAGACATCGGCTGGCTAACcat       <  2:1531210/93‑1 (MQ=255)
                                                                                   aTCATGCCCACTGTTCACGTGGCGGTACCGCCGCATCCAGGCCCTGTCGCCGCAGCGGGGTTACTCCACGCAGACATCGGCTGGCTAACcatc      <  1:16059/93‑1 (MQ=255)
                                                                                     cATGCCCACTGTTCACGTGGCGGTACCGCCGCATCCAGGCCCTGTCGCCGCAGCGGGGTTACTCCACGCAGACATCGGCTGGCTAACcatcat    >  1:459716/1‑93 (MQ=255)
                                                                                     cATGCCCACTGTTCACGTGGCGGTACCGCCGCATCCAGGCCCTGTCGCCGCAGCGGGGTTACTCCACGCAGACATCGGCTGGCTAACcatcat    <  1:1900273/93‑1 (MQ=255)
                                                                                     cATGCCCACTGTTCACGTGGCGGTACCGCCGCATCCAGGCCCTGTCGCCGCAGCGGGGTTACTCCACGCAGACATCGGCTGGCTAACcatcat    >  1:2778353/1‑93 (MQ=255)
                                                                                       tGCCCACTGTTCACGTGGCGGTACCGCCGCATCCAGGCCCTGTCGCCGCAGCGGGGTTACTCCACGCAGACATCGGCTGGCTAACCATCATCg  >  2:6466086/1‑93 (MQ=255)
                                                                                       tGCCCACTGTTCACGTGGCGGTACCGCCGCATCCAGGCCCTGTCGCCGCAGCGGGGTTACTCCACGCAGACATCGGCTGGCTAACCATCATCg  >  1:3519442/1‑93 (MQ=255)
                                                                                          |                                                                                         
TTATTATTCTTGCGCCAATCATTTACGGTTTTGCCAAGGTTGCCAAAATATCGCCACTCAAATTTGGCCTGCCTGTCGCTGGGATCATGCTCACTGTTCACGTGGCGGTACCGCCGCATCCAGGCCCTGTCGCCGCAGCGGGGTTACTCCACGCAGACATCGGCTGGCTAACCATCATCG  >  NC_000913/2863488‑2863667

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 18 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
GCTTTATTATTCTTGCGCCAATCATTTACGGTTTTGCCAAGGTTGCCAAAATATCGCCACTCAAATTTGGCCTGCCTGTCGCTGGGATCATGCTCACTGTTCACGTGGCGGTACCGCCGCATCCAGGCCCTGTCGCCGCAGCGGGGTTACTCCACGCAGACATCGGCTGGCTAACCATCATCGGTAT  >  NC_000913/2863485‑2863671
                                                                                             |                                                                                             
GCTTTATTATTCTTGCGCCAATCATTTACGGTTTTGCCAAGGTTGCCAAAATATCGCCACTCAAATTTGGCCTGCCTGTCGCTGGGATCATGCC                                                                                               >  SRR4302537.2257786/1‑94 (MQ=60)
GCTTTATTATTCTTGCGCCAATCATTTACGGTTTTGCCAAGGTTGCCAAAATATCGCCACTCAAATTTGGCCTGCCTGTCGCTGGGATCATGCC                                                                                               <  SRR4302537.5353039/94‑1 (MQ=60)
GCTTTATTATTCTTGCGCCAATCATTTACGGTTTTGCCAAGGTTGCCAAAATATCGCCACTCAAATTTGGCCTGCCTGTCGCTGGGATCATGCC                                                                                               >  SRR4302537.4362958/1‑94 (MQ=60)
GCTTTATTATTCTTGCGCCAATCATTTACGGTTTTGCCAAGGTTGCCAAAATATCGCCACTCAAATTTGGCCTGCCTGTCGCTGGGATCATGCC                                                                                               >  SRR4302537.4257665/1‑94 (MQ=60)
 CTTTATTATTCTTGCGCCAATCATTTACGGTTTTGCCAAGGTTGCCAAAATATCGCCACTCAAATTTGGCCTGCCTGTCGCTGGGATCATGCCC                                                                                              >  SRR4302537.4933805/1‑94 (MQ=60)
   TTATTATTCTTGCGCCAATCATTTACGGTTTTGCCAAGGTTGCCAAAATATCGCCACTCAAATTTGGCCTGCCTGTCGCTGGGATCATGCCCAC                                                                                            >  SRR4302537.4595610/1‑94 (MQ=60)
     ATTATTCTTGCGCCAATCATTTACGGTTTTGCCAAGGTTGCCAAAATATCGCCACTCAAATTTGGCCTGCCTGTCGCTGGGATCATGCCCACTG                                                                                          >  SRR4302537.5427017/1‑94 (MQ=60)
     ATTATTCTTGCGCCAATCATTTACGGTTTTGCCAAGGTTGCCAAAATATCGCCACTCAAATTTGGCCTGCCTGTCGCTGGGATCATGCCCACTG                                                                                          <  SRR4302537.133620/94‑1 (MQ=60)
      TTATTCTTGCGCCAATCATTTACGGTTTTGCCAAGGTTGCCAAAATATCGCCACTCAAATTTGGCCTGCCTGTCGCTGGGATCATGCCCACTGT                                                                                         >  SRR4302537.3057454/1‑94 (MQ=60)
       TATTCTTGCGCCAATCATTTACGGTTTTGCCAAGGTTGCCAAAATATCGCCACTCAAATTTGGCCTGCCTGTCGCTGGGATCATGCCCACTGTT                                                                                        >  SRR4302537.689557/1‑94 (MQ=60)
        ATTCTTGCGCCAATCATTTACGGTTTTGCCAAGGTTGCCAAAATATCGCCACTCAAATTTGGCCTGCCTGTCGCTGGGATCATGCCCACTGTTC                                                                                       >  SRR4302537.5443913/1‑94 (MQ=60)
         TTCTTGCGCCAATCATTTACGGTTTTGCCAAGGTTGCCAAAATATCGCCACTCAAATTTGGCCTGCCTGTCGCTGGGATCATGCCCACTGTTCA                                                                                      >  SRR4302537.5277550/1‑94 (MQ=60)
           CTTGCGCCAATCATTTACGGTTTTGCCAAGGTTGCCAAAATATCGCCACTCAAATTTGGCCTGCCTGTCGCTGGGATCATGCCCACTGTTCACG                                                                                    >  SRR4302537.4010840/1‑94 (MQ=60)
           CTTGCGCCAATCATTTACGGTTTTGCCAAGGTTGCCAAAATATCGCCACTCAAATTTGGCCTGCCTGTCGCTGGGATCATGCCCACTGTTCACG                                                                                    >  SRR4302537.4074496/1‑94 (MQ=60)
           CTTGCGCCAATCATTTACGGTTTTGCCAAGGTTGCCAAAATATCGCCACTCAAATTTGGCCTGCCTGTCGCTGGGATCATGCCCACTGTTCACG                                                                                    <  SRR4302537.3849737/94‑1 (MQ=60)
              GCGCCAATCATTTACGGTTTTGCCAAGGTTGCCAAAATATCGCCACTCAAATTTGGCCTGCCTGTCGCTGGGATCATGCCCACTGTTCACGTGG                                                                                 <  SRR4302537.814639/94‑1 (MQ=60)
               CGCCAATCATTTACGGTTTTGCCAAGGTTGCCAAAATATCGCCACTCAAATTTGGCCTGCCTGTCGCTGGGATCATGCCCACTGTTCACGTGGC                                                                                >  SRR4302537.6126556/1‑94 (MQ=60)
                GCCAATCATTTACGGTTTTGCCAAGGTTGCCAAAATATCGCCACTCAAATTTGGCCTGCCTGTCGCTGGGATCATGCCCACTGTTCACGTGGCG                                                                               >  SRR4302537.3865353/1‑94 (MQ=60)
                GCCAATCATTTACGGTTTTGCCAAGGTTGCCAAAATATCGCCACTCAAATTTGGCCTGCCTGTCGCTGGGATCATGCCCACTGTTCACGTGGCG                                                                               <  SRR4302537.3858978/94‑1 (MQ=60)
                GCCAATCATTTACGGTTTTGCCAAGGTTGCCAAAATATCGCCACTCAAATTTGGCCTGCCTGTCGCTGGGATCATGCCCACTGTTCACGTGGCG                                                                               <  SRR4302537.10431/94‑1 (MQ=60)
                 CCAATCATTTACGGTTTTGCCAAGGTTGCCAAAATATCGCCACTCAAATTTGGCCTGCCTGTCGCTGGGATCATGCTCACTGTTCACGTGGCGG                                                                              >  SRR4302537.2285243/1‑94 (MQ=60)
                 CCAATCATTTACGGTTTTGCCAAGGTTGCCAAAATATCGCCACTCAAATTTGGCCTGCCTGTCGCTGGGATCATGCCCACTGTTCACGTGGCGG                                                                              <  SRR4302537.1884224/94‑1 (MQ=60)
                 CCAATCATTTACGGTTTTGCCAAGGTTGCCAAAATATCGCCACTCAAATTTGGCCTGCCTGTCGCTGGGATCATGCCCACTGTTCACGTGGCGG                                                                              >  SRR4302537.2883996/1‑94 (MQ=60)
                 CCAATCATTTACGGTTTTGCCAAGGTTGCCAAAATATCGCCACTCAAATTTGGCCTGCCTGTCGCTGGGATCATGCCCACTGTTCACGTGGCGG                                                                              <  SRR4302537.3154992/94‑1 (MQ=60)
                  CAATCATTTACGGTTTTGCCAAGGTTGCCAAAATATCGCCACTCAAATTTGGCCTGCCTGTCGCTGGGATCATGCCCACTGTTCACGTGGCGGT                                                                             >  SRR4302537.4490049/1‑94 (MQ=60)
                  CAATCATTTACGGTTTTGCCAAGGTTGCCAAAATATCGCCACTCAAATTTGGCCTGCCTGTCGCTGGGATCATGCCCACTGTTCACGTGGCGGT                                                                             >  SRR4302537.5008015/1‑94 (MQ=60)
                  CAATCATTTACGGTTTTGCCAAGGTTGCCAAAATATCGCCACTCAAATTTGGCCTGCCTGTCGCTGGGATCATGACCACTGTTCACGcgccgga                                                                             >  SRR4302537.5091843/1‑87 (MQ=60)
                   AATCATTTACGGTTTTGCCAAGGTTGCCAAAATATCGCCACTCAAATTTGGCCTGCCTGTCGCTGGGATCATGCCCACTGTTCACGTGGCGGTA                                                                            >  SRR4302537.2297610/1‑94 (MQ=60)
                   AATCATTTACGGTTTTGCCAAGGTTGCCAAAATATCGCCACTCAAATTTGGCCTGCCTGTCGCTGGGATCATGCCCACTGTTCACGTGGCGGTA                                                                            >  SRR4302537.5218691/1‑94 (MQ=60)
                    ATCATTTACGGTTTTGCCAAGGTTGCCAAAATATCGCCACTCAAATTTGGCCTGCCTGTCGCTGGGATCATGCCCACTGTTCACGTGGCGGTAC                                                                           <  SRR4302537.83428/94‑1 (MQ=60)
                     TCATTTACGGTTTTGCCAAGGTTGCCAAAATATCGCCACTCAAATTTGGCCTGCCTGTCGCTGGGATCATGCTCACTGTTCACGTGGCGGTACC                                                                          >  SRR4302537.5402014/1‑94 (MQ=60)
                     TCATTTACGGTTTTGCCAAGGTTGCCAAAATATCGCCACTCAAATTTGGCCTGCCTGTCGCTGGGATCATGCCCACTGTTCACGTGGCGGTACC                                                                          <  SRR4302537.1163675/94‑1 (MQ=60)
                     TCATTTACGGTTTTGCCAAGGTTGCCAAAATATCGCCACTCAAATTTGGCCTGCCTGTCGCTGGGATCATGCCCACTGTTCACGTGGCGGTACC                                                                          >  SRR4302537.4066921/1‑94 (MQ=60)
                     TCATTTACGGTTTTGCCAAGGTTGCCAAAATATCGCCACTCAAATTTGGCCTGCCTGTCGCTGGGATCATGCCCACTGTTCACGTGGCGGTACC                                                                          >  SRR4302537.4485634/1‑94 (MQ=60)
                      CATTTACGGTTTTGCCAAGGTTGCCAAAATATCGCCACTCAAATTTGGCCTGCCTGTCGCTGGGATCATGCCCACTGTTCACGTGGCGGTACCG                                                                         >  SRR4302537.2270549/1‑94 (MQ=60)
                      CATTTACGGTTTTGCCAAGGTTGCCAAAATATCGCCACTCAAATTTGGCCTGCCTGTCGCTGGGATCATGCCCACTGTTCACGTGGCGGTACCG                                                                         >  SRR4302537.5699800/1‑94 (MQ=60)
                      CATTTACGGTTTTGCCAAGGTTGCCAAAATATCGCCACTCAAATTTGGCCTGCCTGTCGCTGGGATCATGCCCACTGTTCACGTGGCGGTACCG                                                                         >  SRR4302537.5468869/1‑94 (MQ=60)
                       ATTTACGGTTTTGCCAAGGTTGCCAAAATATCGCCACTCAAATTTGGCCTGCCTGTCGCTGGGATCATGCCCACTGTTCACGTGGCGGTACCGC                                                                        >  SRR4302537.3339033/1‑94 (MQ=60)
                        TTTACGGTTTTGCCAAGGTTGCCAAAATATCGCCACTCAAATTTGGCCTGCCTGTCGCTGGGATCATGCCCACTGTTCACGTGGCGGTACCGCC                                                                       >  SRR4302537.581622/1‑94 (MQ=60)
                         TTACGGTTTTGCCAAGGTTGCCAAAATATCGCCACTCAAATTTGGCCTGCCTGTCGCTGGGATCATGCCCACTGTTCACGTGGCGGTACCGCCG                                                                      >  SRR4302537.2696492/1‑94 (MQ=60)
                           ACGGTTTTGCCAAGGTTGCCAAAATATCGCCACTCAAATTTGGCCTGCCTGTCGCTGGGATCATGCCCACTGTTCACGTGGCGGTACCGCCGCA                                                                    >  SRR4302537.3284247/1‑94 (MQ=60)
                            CGGTTTTGCCAAGGTTGCCAAAATATCGCCACTCAAATTTGGCCTGCCTGTCGCTGGGATCATGCTCACTGTTCACGTGGCGGTACCGCCGCAT                                                                   <  SRR4302537.6213253/94‑1 (MQ=60)
                             GGTTTTGCCAAGGTTGCCAAAATATCGCCACTCAAATTTGGCCTGCCTGTCGCTGGGATCATGCCCACTGTTCACGTGGCGGTACCGCCGCATC                                                                  >  SRR4302537.884144/1‑94 (MQ=60)
                             GGTTTTGCCAAGGTTGCCAAAATATCGCCACTCAAATTTGGCCTGCCTGTCGCTGGGATCATGCCCACTGTTCACGTGGCGGTACCGCCGCATC                                                                  >  SRR4302537.1483556/1‑94 (MQ=60)
                             GGTTTTGCCAAGGTTGCCAAAATATCGCCACTCAAATTTGGCCTGCCTGTCGCTGGGATCATGCCCACTGTTCACGTGGCGGTACCGCCGCATC                                                                  >  SRR4302537.4540245/1‑94 (MQ=60)
                             GGTTTTGCCAAGGTTGCCAAAATATCGCCACTCAAATTTGGCCTGCCTGTCGCTGGGATCATGCCCACTGTTCACGTGGCGGTACCGCCGCATC                                                                  >  SRR4302537.3764645/1‑94 (MQ=60)
                              GTTTTGCCAAGGTTGCCAAAATATCGCCACTCAAATTTGGCCTGCCTGTCGCTGGGATCATGCCCACTGTTCACGTGGCGGTACCGCCGCATCC                                                                 >  SRR4302537.3906915/1‑94 (MQ=60)
                              GTTTTGCCAAGGTTGCCAAAATATCGCCACTCAAATTTGGCCTGCCTGTCGCTGGGATCATGCCCACTGTTCACGTGGCGGTACCGCCGCATCC                                                                 >  SRR4302537.899952/1‑94 (MQ=60)
                               TTTTGCCAAGGTTGCCAAAATATCGCCACTCAAATTTGGCCTGCCTGTCGCTGGGATCATGCCCACTGTTCACGTGGCGGTACCGCCGCATCCA                                                                <  SRR4302537.3762552/94‑1 (MQ=60)
                                TTTGCCAAGGTTGCCAAAATATCGCCACTCAAATTTGGCCTGCCTGTCGCTGGGATCATGCCCACTGTTCACGTGGCGGTACCGCCGCATCCAG                                                               >  SRR4302537.5994838/1‑94 (MQ=60)
                                TTTGCCAAGGTTGCCAAAATATCGCCACTCAAATTTGGCCTGCCTGTCGCTGGGATCATGCCCACTGTTCACGTGGCGGTACCGCCGCATCCAG                                                               <  SRR4302537.2352213/94‑1 (MQ=60)
                                    CCAAGGTTGCCAAAATATCGCCACTCAAATTTGGCCTGCCTGTCGCTGGGATCATGCCCACTGTTCACGTGGCGGTACCGCCGCATCCAGGCCC                                                           >  SRR4302537.4899417/1‑94 (MQ=60)
                                     CAAGGTTGCCAAAATATCGCCACTCAAATTTGGCCTGCCTGTCGCTGGGATCATGCCCACTGTTCACGTGGCGGTACCGCCGCATCCAGGCCCT                                                          <  SRR4302537.5983881/94‑1 (MQ=60)
                                      AAGGTTGCCAAAATATCGCCACTCAAATTTGGCCTGCCTGTCGCTGGGATCATGCCCACTGTTCACGTGGCGGTACCGCCGCATCCAGGCCCTG                                                         >  SRR4302537.180643/1‑94 (MQ=60)
                                      AAGGTTGCCAAAATATCGCCACTCAAATTTGGCCTGCCTGTCGCTGGGATCATGCCCACTGTTCACGTGGCGGTACCGCCGCATCCAGGCCCTG                                                         >  SRR4302537.205211/1‑94 (MQ=60)
                                       AGGTTGCCAAAATATCGCCACTCAAATTTGGCCTGCCTGTCGCTGGGATCATGCCCACTGTTCACGTGGCGGTACCGCCGCATCCAGGCCCTGT                                                        <  SRR4302537.1373740/94‑1 (MQ=60)
                                        GGTTGCCAAAATATCGCCACTCAAATTTGGCCTGCCTGTCGCTGGGATCATGCCCACTGTTCACGTGGCGGTACCGCCGCATCCAGGCCCTGTC                                                       <  SRR4302537.55057/94‑1 (MQ=60)
                                         GTTGCCAAAATATCGCCACTCAAATTTGGCCTGCCTGTCGCTGGGATCATGCCCACTGTTCACGTGGCGGTACCGCCGCATCCAGGCCCTGTCG                                                      >  SRR4302537.3530554/1‑94 (MQ=60)
                                           TGCCAAAATATCGCCACTCAAATTTGGCCTGCCTGTCGCTGGGATCATGCCCACTGTTCACGTGGCGGTACCGCCGCATCCAGGCCCTGTCGCC                                                    >  SRR4302537.4461072/1‑94 (MQ=60)
                                           TGCCAAAATATCGCCACTCAAATTTGGCCTGCCTGTCGCTGGGATCATGCCCACTGTTCACGTGGCGGTACCGCCGCATCCAGGCCCTGTCGCC                                                    >  SRR4302537.3666655/1‑94 (MQ=60)
                                           TGCCAAAATATCGCCACTCAAATTTGGCCTGCCTGTCGCTGGGATCATGCCCACTGTTCACGTGGCGGTACCGCCGCATCCAGGCCCTGTCGCC                                                    <  SRR4302537.6042550/94‑1 (MQ=60)
                                            GCCAAAATATCGCCACTCAAATTTGGCCTGCCTGTCGCTGGGATCATGCCCACTGTTCACGTGGCGGTACCGCCGCATCCAGGCCCTGTCGCCG                                                   >  SRR4302537.5899665/1‑94 (MQ=60)
                                              CAAAATATCGCCACTCAAATTTGGCCTGCCTGTCGCTGGGATCATGCCCACTGTTCACGTGGCGGTACCGCCGCATCCAGGCCCTGTCGCCGCA                                                 >  SRR4302537.3758979/1‑94 (MQ=60)
                                               AAAATATCGCCACTCAAATTTGGCCTGCCTGTCGCTGGGATCATGCCCACTGTTCACGTGGCGGTACCGCCGCATCCAGGCCCTGTCGCCGCAG                                                >  SRR4302537.5058826/1‑94 (MQ=60)
                                                 AATATCGCCGCTCAAATTTGGCCTGCCTGTCGCTGGGATCATGCCCACTGTTCACGTGGCGGTACCGCCGCATCCAGGCCCTGTCGCCGCAGCG                                              <  SRR4302537.435289/94‑1 (MQ=60)
                                                  ATATCGCCACTCAAATTTGGCCTGCCTGTCGCTGGGATCATGCCCACTGTTCACGTGGCGGTACCGCCGCATCCAGGCCCTGTCGCCGCAGCGG                                             <  SRR4302537.5769367/94‑1 (MQ=60)
                                                  ATATCGCCACTCAAATTTGGCCTGCCTGTCGCTGGGATCATGCCCACTGTTCACGTGGCGGTACCGCCGCATCCAGGCCCTGTCGCCGCAGCGG                                             >  SRR4302537.5120548/1‑94 (MQ=60)
                                                   TATCGCCACTCAAATTTGGCCTGCCTGTCGCTGGGATCATGCCCACTGTTCACGTGGCGGTACCGCCGCATCCAGGCCCTGTCGCCGCAGCGGG                                            <  SRR4302537.3985246/94‑1 (MQ=60)
                                                   TATCGCCACTCAAATTTGGCCTGCCTGTCGCTGGGATCATGCCCACTGTTCACGTGGCGGTACCGCCGCATCCAGGCCCTGTCGCCGCAGCGGG                                            <  SRR4302537.3985242/94‑1 (MQ=60)
                                                    ATCGCCACTCAAATTTGGCCTGCCTGTCGCTGGGATCATGCCCACTGTTCACGTGGCCGTACCGCCGCATCCTGGCCCTGTCGCCGCAGCGGGG                                           >  SRR4302537.796490/1‑94 (MQ=60)
                                                     TCGCCACTCAAATTTGGCCTGCCTGTCGCTGGGATCATGCTCACTGTTCACGTGGCGGTACCGCCGCATCCAGGCCCTGTCGCCGCAGCGGGGT                                          <  SRR4302537.6092057/94‑1 (MQ=60)
                                                      CGCCACTCAAATTTGGCCTGCCTGTCGCTGGGATCATGCCCACTGTTCACGTGGCGGTACCGCCGCATCCAGGCCCTGTCGCCGCAGCGGGGTT                                         >  SRR4302537.3171380/1‑94 (MQ=60)
                                                       GCCACTCAAATTTGGCCTGCCTGTCGCTGGGATCATGCCCACTGTTCACGTGGCGGTACCGCCGCATCCAGGCCCTGTCGCCGCAGCGGGGTTA                                        <  SRR4302537.1408313/94‑1 (MQ=60)
                                                        CCACTCAAATTTGGCCTGCCTGTCGCTGGGATCATGCCCACTGTTCACGTGGCGGTACCGCCGCATCCAGGCCCTGTCGCCGCAGCGGGGTTAC                                       >  SRR4302537.4101791/1‑94 (MQ=60)
                                                        CCACTCAAATTTGGCCTGCCTGTCGCTGGGATCATGCCCACTGTTCACGTGGCGGTACCGCCGCATCCAGGCCCTGTCGCCGCAGCGGGGTTAC                                       >  SRR4302537.3648440/1‑94 (MQ=60)
                                                         CACTCAAATTTGGCCTGCCTGTCGCTGGGATCATGCCCACTGTTCACGTGGCGGTACCGCCGCATCCAGGCCCTGTCGCCGCAGCGGGGTTACT                                      <  SRR4302537.5766778/94‑1 (MQ=60)
                                                         CACTCAAATTTGGCCTGCCTGTCGCTGGGATCATGCCCACTGTTCACGTGGCGGTACCGCCGCATCCAGGCCCTGTCGCCGCAGCGGGGTTACT                                      >  SRR4302537.3524535/1‑94 (MQ=60)
                                                         CACTCAAATTTGGCCTGCCTGTCGCTGGGATCATGACCACTGTTCACGTGGCGGTACCGCCGCATCCAGGCCCTGTCGCCGCAGCGGGGTTAAT                                      >  SRR4302537.2572928/1‑94 (MQ=60)
                                                          ACTCAAATTTGGCCTGCCTGTCGCTGGGATCATGCTCACTGTTCACGTGGCGGTACCGCCGCATCCAGACCCTGTCGCCGCAGCGGGGTTACTC                                     >  SRR4302537.6291616/1‑94 (MQ=60)
                                                          ACTCAAATTTGGCCTGCCTGTCGCTGGGATCATGCCCACTGTTCACGTGGCGGTACCGCCGCATCCAGGCCCTGTCGCCGCAGCGGGGTTACTC                                     >  SRR4302537.874511/1‑94 (MQ=60)
                                                             CAAATTTGGCCTGCCTGTCGCTGGGATCATGCCCACTGTTCACGTGGCGGTACCGCCGCATCCAGGCCCTGTCGCCGCAGCGGGGTTACTCCAC                                  >  SRR4302537.3703115/1‑94 (MQ=60)
                                                              AAATTTGGCCTGCCTGTCGCTGGGATCATGCCCACTGTTCACGTGGCGGTACCGCCGCATCCAGGCCCTGTCGCCGCAGCGGGGTTACTCCACG                                 >  SRR4302537.3176679/1‑94 (MQ=60)
                                                               AATTTGGCCTGCCTGTCGCTGGGATCATGCCCACTGTTCACGTGGCGGTACCGCCGCATCCAGGCCCTGTCGCCGCAGCGGGGTTACTCCACGC                                >  SRR4302537.3482853/1‑94 (MQ=60)
                                                                ATTTGCCCTGCCTGTCGCTGGGATCATGCCCACTGTTCACGTGGCGGTACCGCCGCATCCAGGCCCTGTCGCCGCAGCGGGGTTACTCCACGCA                               <  SRR4302537.5681914/94‑1 (MQ=60)
                                                                 TTTCGCCTGCCTGTCGCTGGGATCATGCCCACTGTTCACGTGGCGGTACCGCCGCATCCAGGCCCTGTCGCCGCAGCGGGGTTACTACACGCAG                              <  SRR4302537.321561/94‑1 (MQ=60)
                                                                   TGGCCTGCCTGTCGCTGGGATCATGCCCACTGTTCACGTGGCGGTACCGCCGCATCCAGGCCCTGTCGCCGCAGCGGGGTTACTCCACGCAGAC                            >  SRR4302537.1590916/1‑94 (MQ=60)
                                                                   TGGCCTGCCTGTCGCTGGGATCATGCCCACTGTTCACGTGGCGGTACCGCCGCATCCAGGCCCTGTCGCCGCAGCGGGGTTACTCCACGCAGAC                            <  SRR4302537.4592973/94‑1 (MQ=60)
                                                                     GCCTGCCTGTCGCTGGGATCATGCCCACTGTTCACGTGGCGGTACCGCCGCATCCAGGCCCTGTCGCCGCAGCGGGGTTACTCCACGCAGACAT                          >  SRR4302537.396956/1‑94 (MQ=60)
                                                                     GCCTGCCTGTCGCTGGGATCATGCCCACTGTTCACGTGGCGGTACCGCCGCATCCAGGCCCTGTCGCCGCAGCGGGGTTACTCCACGCAGACAT                          >  SRR4302537.602746/1‑94 (MQ=60)
                                                                     GCCTGCCTGTCGCTGGGATCATGCCCACTGTTCACGTGGCGGTACCGCCGCATCCAGGCCCTGTCGCCGCAGCGGGGTTACTCCACGCAGACAT                          <  SRR4302537.5400187/94‑1 (MQ=60)
                                                                      CCTGCCTGTCGCTGGGATCATGCCCACTGTTCACGTGGCGGTACCGCCGCATCCAGGCCCTGTCGCCGCAGCGGGGTTACTCCACGCAGACATC                         <  SRR4302537.659949/94‑1 (MQ=60)
                                                                      CCTGCCTGTCGCTGGGATCATGCCCACTGTTCACGTGGCGGTACCGCCGCATCCAGGCCCTGTCGCCGCAGCGGGGTTACTCCACGCAGACATC                         >  SRR4302537.1058309/1‑94 (MQ=60)
                                                                      CCTGCCTGTCGCTGGGATCATGCCCACTGTTCACGTGGCGGTACCGCCGCATCCAGGCCCTGTCGCCGCAGCGGGGTTACTCCACGCAGACATC                         <  SRR4302537.4874922/94‑1 (MQ=60)
                                                                      CCTGCCTGTCGCTGGGATCATGCCCACTGTTCACGTGGCGGTACCGCCGCATCCAGGCCCTGTCGCCGCAGCGGGGTTACTCCACGCAGACATC                         >  SRR4302537.350586/1‑94 (MQ=60)
                                                                      CCTGCCTGTCGCTGGGATCATGCCCACTGTTCACGTGGCGGTACCGCCGCATCCAGGCCCTGTCGCCGCAGCGGGGTTACTCCACGCAGACATC                         <  SRR4302537.3590273/94‑1 (MQ=60)
                                                                      CCTGCCTGTCGCTGGGATCATGCCCACTGTTCACGTGGCGGTACCGCCGCATCCAGGCCCTGTCGCCGCAGCGGGGGTACTCCACGCAGACATC                         >  SRR4302537.714662/1‑94 (MQ=60)
                                                                      CCTGCCTGTCGCTGGGATCATGCCCACTGTTCACGTGGCGGTACCGCCGCATCCAGGCCCTGTCGCCGCAGCGGGGCTACTCCACGCAGACATC                         >  SRR4302537.1781119/1‑94 (MQ=60)
                                                                      CCTGCCTGTCGCTGGGATCATGCCCACTGTTCAAGTGGCGGTACCGCCGCATCCAGGCCCTGTCGCCGCAGCGGGGTTACTCCACGCAGACATC                         <  SRR4302537.5852148/94‑1 (MQ=60)
                                                                       CTGCCTGTCGCTGGGATCATGCCCACTGTTCACGTGGCGGTACCGCCGCATCCAGGCCCTGTCGCCGCAGCGGGGTTACTCCACGCAGACATCG                        >  SRR4302537.3556190/1‑94 (MQ=60)
                                                                         GCCTGTCGCTGGGATCATGCCCACTGTTCACGTGGCGGTACCGCCGCATCCAGGCCCTGTCGCCGCAGCGGGGTTACTCCACGCAGACATCGGC                      <  SRR4302537.670932/94‑1 (MQ=60)
                                                                          CCTGTCGCTGGGATCATGCCCACTGTTCACGTGGCGGTACCGCCGCATCCAGGCCCTGTCGCCGCAGCGGGGTTACTCCACGCAGACATCGGCT                     <  SRR4302537.5188029/94‑1 (MQ=60)
                                                                            TGTCGCTGGGATCATGCCCACTGTTCACGTGGCGGTACCGCCGCATCCAGGCCCTGTCGCCGCAGCGGGGTTACTCCACGCAGACATCGGCTGG                   >  SRR4302537.6176481/1‑94 (MQ=60)
                                                                            TGTCGCTGGGATCATGCCCACTGTTCACGTGGCGGTACAGCCGCATCCAGGCCCTGTCGCCGCAGCGGGGTTACTGCACGCAGACATCGGCTGG                   >  SRR4302537.546963/1‑94 (MQ=60)
                                                                              TCGCTGGGATCATGCCCACTGTTCACGTGGCGGTACCGCCGCATCCAGGCCCTGTCGCCGCAGCGGGGTTACTCCACGCAGACATCGGCTGGCT                 <  SRR4302537.985692/94‑1 (MQ=60)
                                                                              TCGCTGGGATCATGCCCACTGTTCACGTGGCGGTACCGCCGCATCCAGGCCCTGTCGCCGCAGCGGGGTTACTCCACGCAGACATCGGCTGGCT                 <  SRR4302537.5306497/94‑1 (MQ=60)
                                                                              TCGCTGGGATCATGCCCACTGTTCACGTGGCGGTACCGCCGCATCCAGGCCCTGTCGCCGCAGCGGGGTTACTCCACGCAGACATCGGCTGGCT                 <  SRR4302537.2331875/94‑1 (MQ=60)
                                                                              TCGCTGGGATCATGCCCACTGTTCACGTGGCGGTACCGCCGCATCCAGGCCCTGTCGCCGCAGCGGGGTTACTCCACGCAGACATCGGCTGGCT                 >  SRR4302537.6002421/1‑94 (MQ=60)
                                                                              GCGCTGGGATCATGCCCACTGTTCACGTGGCGGTACCGCCGCATCCAGGCCCTGTCGCCGCAGCGGGGTTACTCCACGCAGACATCGGCTGGCT                 <  SRR4302537.5997325/94‑1 (MQ=60)
                                                                                GCTGGGATCATGCCCACTGTTCACGTGGCGGTACCGCCGCATCCAGGCCCTGTCGCCGCAGCGGGGTTACTCCACGCAGACATCGGCTGGCTAA               >  SRR4302537.5619251/1‑94 (MQ=60)
                                                                                GCTGGGATCATGCCCACTGTTCACGTGGCGGTACCGCCGCATCCAGGCCCTGTCGCCGCAGCGGGGTTACTCCACGCAGACATCGGCTGGCTAA               >  SRR4302537.301815/1‑94 (MQ=60)
                                                                                  TGGGATCATGCCCACTGTTCACGTGGCGGTACCGCCGCATCCAGGCCCTGTCGCCGCAGCGGGGTTACTCCACGCAGACATCGGCTGGGTAACC             >  SRR4302537.4192283/1‑94 (MQ=60)
                                                                                  TGGGATCATGCCCACTGTTCACGTGGCGGTACCGCCGCATCCAGGCCCTGTCGCCGCAGCGGGGTTACTCCACGCAGACATCGGCTGGCTAACC             >  SRR4302537.906360/1‑94 (MQ=60)
                                                                                  TGGGATCATGCCCACTGTTCACGTGGCGGTACCGCCGCATCCAGGCCCTGTCGCCGCAGCGGGGTTACTCCACGCAGACATCGGCTGGCTAACC             >  SRR4302537.3468148/1‑94 (MQ=60)
                                                                                  TGGGATCATGCCCACTGTTCACGTGGCGGTACCGCCGCATCCAGGCCCTGTCGCCGCAGCGGGGTTACTCCACGCAGACATCGGCTGGCTAACC             >  SRR4302537.342410/1‑94 (MQ=60)
                                                                                  TGGGATCATGCCCACTGTTCACGTGGCGGTACCGCCGCATCCAGGCCCTGTCGCCGCAGCGGGGTTACTCCACGCAGACATCGGCTGGCTAACC             <  SRR4302537.4730957/94‑1 (MQ=60)
                                                                                  TGGGATCATGCCCACTGTTCACGTGGCGGTACCGCCGCATCCAGGCCCTGTCGCCGCAGCGGGGTTACTACACGCAGACATCGGCTGGCTAACC             <  SRR4302537.4177591/94‑1 (MQ=60)
                                                                                    GGATCATGCCCACTGTTCACGTGGCGGTACCGCCGCATCCAGGCCCTGTCGCCGCAGCGGGGTTACTCCACGCAGACATCGGCTGGCTAACCAT           >  SRR4302537.3964997/1‑94 (MQ=60)
                                                                                    GGATCATGCCCACTGTTCACGTGGCGGTACCGCCGCATCCAGGCCCTGTCGCCGCAGCGGGGTTACTCCACGCAGACATCGGCTGGCTAACCAT           >  SRR4302537.5966343/1‑94 (MQ=60)
                                                                                    GGATCATGCCCACTGTTCACGTGGCGGTACCGCCGCATCCAGGCCCTGTCGCCGCAGCGGGGTTACTCCACGCAGACATCGGCTGGCTAACCAT           <  SRR4302537.5657816/94‑1 (MQ=60)
                                                                                     GATCATGCCCACTGTTCACGTGGCGGTACCGCCGCATCTAGGCCCTGTCGCCGCAGCGGGGTTACTCCACGCAGACATCGGCTGGCTAACCATC          <  SRR4302537.2114338/94‑1 (MQ=60)
                                                                                      ATCATGCCCACTGTTCACGTGGCGGTACCGCCGCATCCAGGCCCTGTCGCCGCAGCGGGGTTACTCCACGCAGACATCGGCTGGCTAACCATCA         <  SRR4302537.16190/94‑1 (MQ=60)
                                                                                       TCATGCCCACTGTTCACGTGGCGGTACCGCCGCATCCAGGCCCTGTCGCCGCAGCGGGGTTACTCCACGCAGACATCGGCTGGCTAACCATCAT        >  SRR4302537.2797778/1‑94 (MQ=60)
                                                                                       TCATGCCCACTGTTCACGTGGCGGTACCGCCGCATCCAGGCCCTGTCGCCGCAGCGGGGTTACTCCACGCAGACATCGGCTGGCTAACCATCAT        >  SRR4302537.462882/1‑94 (MQ=60)
                                                                                        CATGCCCACTGTTCACGTGGCGGTACCGCCGCATCCAGGCCCTGTCGCCGCAGCGGGGTTACTCCACGCAGACATCGGCTGGCTAACCATCATC       <  SRR4302537.1913471/94‑1 (MQ=60)
                                                                                         ATGCCCACTGTTCACGTGGCGGTACCGCCGCATCCAGGCCCTGTCGCCGCAGCGGGGTTACTCCACGCAGACATCGGCTGGCTAACCATCATCG      <  SRR4302537.1216828/94‑1 (MQ=60)
                                                                                         ATGCCCACTGTTCACGTGGCGGTACCGCCGCATCCAGGCCCTGTCGCCGCAGCGGGGTTACTCCACGCAGACATCGGCTGGCTAACCATCATCG      >  SRR4302537.3544345/1‑94 (MQ=60)
                                                                                          TGCCCACTGTTCACGTGGCGGTACCGCCGCATCCAGGCCCTGTCGCCGCAGCGGGGTTACTCCACGCAGACATCGGCTGGCTAACCATCATCGG     >  SRR4302537.2533794/1‑94 (MQ=60)
                                                                                           GCCCACTGTTCACGTGGCGGTACCGCCGCATCCAGGCCCTGTCGCCGCAGCGGGGTTACTCCACGCAGACATCGGCTGGCTAACCATCATCGGT    >  SRR4302537.4688334/1‑94 (MQ=60)
                                                                                            CCCACTGTTCACGTGGCGGTACCGCCGCATCCAGGCCCTGTCGCCGCAGCGGGGTTACTCCACGCAGACATCGGCTGGCTAACCATCATCGGTA   <  SRR4302537.4803942/94‑1 (MQ=60)
                                                                                             CCACTGTTCACGTGGCGGTACCGCCGCATCCAGGCCCTGTCGCCGCAGCGGGGTTACTCCACGCAGACATCGGCTGGCTAACCATCATCGGTAT  <  SRR4302537.3327659/94‑1 (MQ=60)
                                                                                             |                                                                                             
GCTTTATTATTCTTGCGCCAATCATTTACGGTTTTGCCAAGGTTGCCAAAATATCGCCACTCAAATTTGGCCTGCCTGTCGCTGGGATCATGCTCACTGTTCACGTGGCGGTACCGCCGCATCCAGGCCCTGTCGCCGCAGCGGGGTTACTCCACGCAGACATCGGCTGGCTAACCATCATCGGTAT  >  NC_000913/2863485‑2863671

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 10 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑