Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A1 F1 I2 R1	882	254.4	12957588	98.0%	12698436	93.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,894,456	A→G	G41G (GGT→GGC)	b2770 ←	putative transport protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,894,456	0	A	G	99.5%	655.0 / ‑4.5	194	G41G (GGT→GGC)	b2770	putative transport protein
Reads supporting (aligned to +/- strand): ref base A (0/1); new base G (101/92); total (101/93)
Fisher's exact test for biased strand distribution p-value = 4.79e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.52e-01

Only 100 of 235 total aligned reads displayed.

TTCATCCCCCATGCTCAACGCGGTTAAAGACATCGGCGTACCGTTTTTTCTCTGCGCCAACAGCAGCGCGGCAGCGGCCTGTTCATCAGCACCGAGTAAACTTCGCAGTAGCGAAATATCCGGTCCGCTTTTACCCTGAGCCGCCGCCTGCCACTCTTTTTCCGCCAGCATTCCGGCATC  >  NC_000913/2894366‑2894545

ttCATCCCCCATGCTCAACGCGGTTAAAGACATCGGCGTACCGTTTTTTCTCTGCGCCAACAGCAGCGCGGCAGCGGCCTGTTCCTCAGCGcc                                                                                         >  2:25148/1‑93 (MQ=255)

ttCATCCCCCATGCTCAACGCGGTTAAAGACATCGGCGTACCGTTTTTTCTCTGCGCCAACAGCAGCGCGGCAGCGGCCTGTTCATCAGCGcc                                                                                         >  2:3459849/1‑93 (MQ=255)

   aTCCCCCATGCTCAACGCGGTTAAAGACATCGGCGTACCGTTTTTTCTCTGCGCCAACAGCAGCGCGGCAGCGGCCTGTTCATCAGCGCCGAg                                                                                      <  2:4935575/93‑1 (MQ=255)

    tCCCCCATGCTCAACGCGGTTAAAGACATCGGCGTACCGTTTTTTCTCTGCGCCAACAGCAGCGCGGCAGCGGCCTGTTCATCAGCGCCGAGt                                                                                     <  1:141465/93‑1 (MQ=255)

    tCCCCCATGCTCAACGCGGTTAAAGACATCGGCGTACCGTTTTTTCTCTGCGCCAACAGCAGCGCGGCAGCGGCCTGTTCATCAGCGCCGAGt                                                                                     <  1:4738912/93‑1 (MQ=255)

      ccccATGCTCAACGCGGTTAAAGACATCGGCGTACCGTTTTTTCTCTGCGCCAACAGCAGCGCGGCAGCGGCCTGTTCATCAGCGCCGAGTaa                                                                                   <  2:5531694/93‑1 (MQ=255)

      ccccATGCTCAACGCGGTTAAAGACATCGGCGTACCGTTTTTTCTCTGCGCCAACAGCAGCGCGGCAGAGGCCTGCTCATCAGCGGCGAGTaa                                                                                   >  1:3801176/1‑93 (MQ=255)

       cccATGCTCAACGCGGTTAAAGACATCGGCGTACCGTTTTTTCTCTGCGCCAACAGCAGCGCGGCAGCGGCCTGTTCATCAGCGCCGAGTaaa                                                                                  <  2:5418309/93‑1 (MQ=255)

       cccATGCTCAACGCGGTTAAAGACATCGGCGTACCGTTTTTTCTCTGCGCCAACAGCAGCGCGGCAGCGGCCTGTTCATCAGCGCCGAGTaaa                                                                                  <  1:216087/93‑1 (MQ=255)

        ccATGCTCAACGCGGTTAAAGACATCGGCGTACCGTTTTTTCTCTGCGCCAACAGCAGCGCGGCAGCGGCCTGTTCATCAGCGCCGAGTAAAc                                                                                 <  1:5511248/93‑1 (MQ=255)

        ccATGCTCAACGCGGTTAAAGACATCGGCGTACCGTTTTTTCTCTGCGCCAACAGCAGCGCGGCAGCGGCCTGTTCATCAGCGCCGAGTAAAc                                                                                 <  2:2284226/93‑1 (MQ=255)

         cATGCTCAACGCGGTTAAAGACATCGGCGTACCGTTTTTTCTCTGCGCCAACAGCAGCGCGGCAGCGGCCTGTTTATCAGCGCCGAGTAAACt                                                                                >  2:4542119/1‑93 (MQ=255)

         cATGCTCAACGCGGTTAAAGACATCGGCGTACCGTTTTTTCTCTGCGCCAACAGCAGCGCGGCAGCGGCCTGTTCATCAGCGCCGAGTAAACt                                                                                >  2:4797279/1‑93 (MQ=255)

          aTGCTCAACGCGGTTAAAGACATCGGCGTACCGTTTTTTCTCTGCGCCAACAGCAGCGCGGCAGCGGCCTGTTCATCAGCGCCGAGTAAACtt                                                                               <  2:5844938/93‑1 (MQ=255)

          aTGCTCAACGCGGTTAAAGACATCGGCGTACCGTTTTTTCTCTGCGCCAACAGCAGCGCGGCAGCGGCCTGTTCATCAGCGCCGAGGAAACtt                                                                               >  2:5570555/1‑93 (MQ=255)

           tGCTCAACGCGGTTAAAGACATCGGCGTACCGTTTTTTCTCTGCGCCAACAGCAGCGCGGCAGCGGCCTGTTCATCAGCGCCGAGTAAACTTc                                                                              >  2:782734/1‑93 (MQ=255)

           tGCTCAACGCGGTTAAAGACATCGGCGTACCGTTTTTTCTCTGCGCCAACAGCAGCGCGGCAGCGGCCTGTTCATCAGCGCCGAGTAAACTTc                                                                              >  1:2122143/1‑93 (MQ=255)

           tGCTCAACGCGGTTAAAGACATCGGCGTACCGTTTTTTCTCTGCGCCAACAGCAGCGCGGCAGCGGCCTGTTCATCAGCGCCGAGTAAACTTc                                                                              >  1:1851410/1‑93 (MQ=255)

           tGCTCAACGCGGTTAAAGACATCGGCGTACCGTTTTTTCTCTGCGCCAACAGCAGCGCGGCAGCGGCCTGTTCATCAGCGCCGAGTAAACTTc                                                                              <  2:1773684/93‑1 (MQ=255)

            gCTCAACGCGGTTAAAGACATCGGCGTACCGTTTTTTCTCTGCGCCAACAGCAGCGCGGCAGCGGCCTGTTCATCAGCGCCGAGTAAACTTCg                                                                             <  1:711066/93‑1 (MQ=255)

            gCTCAACGCGGTTAAAGACATCGGCGTACCGTTTTTTCTCTGCGCAAACAGCAGCGCGGCAGCGTCCTGTTCATCAGCGCCGAGTAAACTTCg                                                                             <  2:3377328/93‑1 (MQ=255)

             cTCAACGCGGTTAAAGACATCGGCGTACCGTTTTTTCTCTGCGCCAACAGCAGCGCGGCAGCGGCCTGTTCATCAGCGCCGAGTAAACTTCGc                                                                            <  1:2346724/93‑1 (MQ=255)

             cTCAACGCGGTTAAAGACATCGGCGTACCGTTTTTTCTCTGCGCCAACAGCAGCGCGGCAGCGGCCTGTTCATCAGCGCCGAGTAAACTTCGc                                                                            <  1:1522746/93‑1 (MQ=255)

              tCAACGCGGTTAAAGACATCGGCGTACCGTTTTTTCTCTGCGCCAACAGCAGCGCGGCAGCGGCCTGTTCATCAGCGCCGAGTAAACTTCGCa                                                                           >  1:1568148/1‑93 (MQ=255)

                aaCGCGGTTAAAGACATCGGCGTACCGTTTTTTCTCTGCGCCAACAGCAGCGCGGCAGCGGCCTGTTCATCAGCGCCGAGTAAACTTCGCAGt                                                                         >  2:6053614/1‑93 (MQ=255)

                aaCGCGGTTAAAGACATCGGCGTACCGTTTTTTCTCTGCGCCAACAGCAGCGCGGCAGCGGCCTGTTCATCAGCGCCGAGTAAACTTCGCAGt                                                                         >  1:1610966/1‑93 (MQ=255)

                 aCGCGGTTAAAGACATCGGCGTACCGTTTTTTCTCTGCGCCAACAGCAGCGCGGCAGCGGCCTGTTCATCAGCGCCGAGTAAACTTCGCAGTa                                                                        <  2:2255810/93‑1 (MQ=255)

                 aCGCGGTTAAAGACATCGGCGTACCGTTTTTTCTCTGCGCCAACAGCAGCGCGGCAGCGGCCTGTTCATCAGCGCCGAGTAAACTTCGCAGTa                                                                        >  1:2776823/1‑93 (MQ=255)

                 aCGCGGTTAAAGACATCGGCGTACCGTTTTTTCTCTGCGCCAACAGCAGCGCGGCAGCGGCCTGTTCATCAGCGCCGAGTAAACTTCGCAGTa                                                                        <  2:4056310/93‑1 (MQ=255)

                   gcgGTTAAAGACATCGGCGTACCGTTTTTTCTCTGCGCCAACAGCAGCGCGGCAGCGGCCTGTTCATCAGCGCCGAGTAAACTTCGCAGTAGc                                                                      >  1:782357/1‑93 (MQ=255)

                     ggTTAAAGACATCGGCGTACCGTTTTTTCTCTGCGCCAACAGCAGCGCGGCAGCGGCCTGTTCATCAGCGCCGAGTAAACTTCGCAGTAGCGa                                                                    >  2:4170245/1‑93 (MQ=255)

                     ggTTAAAGACATCGGCGTACCGTTTTTTCTCTGCGCCAACAGCAGCGCGGCAGCGGCCTGTTCATCAGCGCCGAGTAAACTTCGCAGTAGCGa                                                                    >  2:5753185/1‑93 (MQ=255)

                     ggTTAAAGACATCGGCGTACCGTTTTTTCTCTGCGCCAACAGCAGCGCGGCAGCGGCCTGCTTCTCAGCGCCGAAAAAAACTCGCAAAAGCgc                                                                    >  1:13105/1‑92 (MQ=255)

                        tAAAGACATCGGCGTACCGTTTTTTCTCTGCGCCAACAGCAGCGCGGCAGCGGCCTGTTCATCAGCGCCGAGTAAACTTCGCAGTAGCGAAat                                                                 >  2:2581566/1‑93 (MQ=255)

                          aaGACATCGGCGTACCGTTTTTTCTCTGCGCCAACAGCAGCGCGGCAGCGGCCTGTTCATCAGCGCCGAGTAAACTTCGCAGTAGCGAAatat                                                               >  2:823347/1‑93 (MQ=255)

                           aGACATCGGCGTACCGTTTTTTCTCTGCGCCAACAGCAGCGCGGCAGCGGCCTGTTCATCAGCGCCGAGTAAACTTCGCAGTAGCGAAATATc                                                              >  1:154864/1‑93 (MQ=255)

                            gACATCGGCGTACTGTTTTTTCTCTGCGCCAACAGCAGCGCGGCAGCGGCCTGTTCATCAGCGCCGAGTAAACTTCGCAGTAGCGAAATATcc                                                             <  2:626123/93‑1 (MQ=255)

                            gACATCGGCGTACCGTTTTTTCTCTGCGCCAACAGCAGCGCGGCAGCGGCCTGTTCATCAGCGCCGAGTAAACTTCGCAGTAGCTAAATATcc                                                             <  2:6285891/93‑1 (MQ=255)

                            gACATCGGCGTACCGTTTTTTCTCTGCGCCAACAGCAGCGCGGCAGCGGCCTGTTCATCAGCGCCGAGTAAACTTCGCAGTAGCGAAATATcc                                                             <  1:5217213/93‑1 (MQ=255)

                            gACATCGGCGTACCGTTTTTTCTCTGCGCCAACAGCAGCGCGGCAGCGGCCTGTTCATCAGCGCCGAGTAAACTTCGCAGTAGCGAAATATcc                                                             >  2:2150717/1‑93 (MQ=255)

                             aCATCGGCGTACCGTTTTTTCTCTGCGCCAACAGCAGCGCGGCAGCGGCCTGTTCATCAGCGCCGAGTAAACTTCGCAGTAGCGAAATATCCg                                                            <  2:3214647/93‑1 (MQ=255)

                             aCATCGGCGTACCGTTTTTTCTCTGCGCCAACAGCAGCGCGGCAGCGGCCTGTTCATCAGCGCCGAGTAAACTTCGCAGTAGCGAAATATCCg                                                            >  1:4042180/1‑93 (MQ=255)

                              cATCGGCGTACCGTTTTTTCTCTGCGCCAACAGCAGCGCGGCAGCGGCCTGTTCATCAGCGGCGAGTAAAGCTCGCAGTAGCGCAATATACgg                                                           >  1:4455256/1‑93 (MQ=255)

                              cATCGGCGTACCGTTTTTTCTCTGCGCCAACAGCAGCGCGGCAGCGGCCTGTTCATCAGCGCCGAGTAAACTTCGCAGTAGCGAAATATCCgg                                                           <  1:1246501/93‑1 (MQ=255)

                              cATCGGCGTACCGTTTTTTCTCTGCGCCAACAGCAGCGCGGCAGCGGCCTGTTCATCAGCGCCGAGTAAACTTCGCAGTAGCGAAATATCCgg                                                           <  2:3777984/93‑1 (MQ=255)

                              cATCGGCGTACCGTTTTTTCTCTGCGCCAACAGCAGCGCGGCAGCGGCCTGTTCATCAGCGCCGAGTAAACTTCGCAGTAGCGAAATATCCgg                                                           >  1:1598707/1‑93 (MQ=255)

                              cATCGGCGTACCGTTTTTTCTCTGCGCCAACAGCAGCGCGGCAGCGGCCTGTTCATCAGCGCCGAGTAAACTTCGCAGTAGCGAAATATCCgg                                                           <  1:3182042/93‑1 (MQ=255)

                               aTCGGCGTACCGTTTTTTCTCTGCGCCAACAGCAGCGCGGCAGCGGCCTGTTCATCAGCGCCGAGTAAACTTCGCAGTAGCGAAATATCCGGt                                                          >  1:6006719/1‑93 (MQ=255)

                                tCGGCGTACCGTTTTTTCTCTGCGCCAACAGCAGCGCGGCAGCGGCCTGTTCATCAGCGCCGAGTAAACTTCGCAGTAGCGAAATATCCGGTc                                                         >  2:2555363/1‑93 (MQ=255)

                                tCGGCGTACCGTTTTTTCTCTGCGCCAACAGCAGCGCGGCAGCGGCCTGTTCATCAGCGCCGAGTAAACTTCGCAGTAGCGAAATATCCGGTc                                                         <  1:5574250/93‑1 (MQ=255)

                                   gCGTACCGTTTTTTCTCTGCGCCAACAGCAGCGCGGCAGCGGCCTGTTCATCAGCGCCGAGTAAACTTCGCAGTAGCGAAATATCCGGTCCGc                                                      <  2:1715394/93‑1 (MQ=255)

                                   gCGTACCGTTTTTTCTCTGCGCCAACAGCAGCGCGGCAGCGGCCTGTTCATCAGCGCCGAGTAAACTTCGCAGTAGCGAAATATCCGGTCCGc                                                      >  2:3338205/1‑93 (MQ=255)

                                   gCGTACCGTTTTTTCTCTGCGCCAACAGCAGCGCGGCAGCGGCCTGTTCATCAGCGCCGAGTAAACTTCGCAGTAGCGAAATATCCGGTCCGc                                                      <  1:5893235/93‑1 (MQ=255)

                                    cGTACCGTTTTTTCTCTGCGCCAACAGCAGCGCGGCAGCGGCCTGTTCATCAGCGCCGAGTAAACTTCGCAGTAGCGAAATATCCGGGCCGCt                                                     >  2:2307666/1‑93 (MQ=255)

                                        ccGTTTTTTCTCTGCGCCAACAGCAGCGCGGCAGCGGCCTGTTCATCAGCGCCGAGTAAACTTCGCAGTAGCGAAATATCCGGTCCGCTTTTa                                                 >  2:5392565/1‑93 (MQ=255)

                                        ccGTTTTTTCTCTGCGCCAACAGCAGCGCGGCAGCGGCCTGTTCATCAGCGCCGAGTAAACTTCGCAGTAGCGAAATATCCGGTCCGCTTTTa                                                 <  1:5155833/93‑1 (MQ=255)

                                         cGTTTTTTCTCTGCGCCAACAGCAGCGCGGCAGCGGCCTGTTCATCAGCGCCGAGTAAACTTCGCAGTAGCGAAATATCCGGTCCGCTTTTAc                                                >  1:2449335/1‑93 (MQ=255)

                                          gTTTTTTCTCTGCGCCAACAGCAGCGCGGCAGCGGCCTGTTCATCAGCGCCGAGTAAACTTCGCAGTAGCGAAATATCCGGTCCGCTTTTAcc                                               >  2:1488257/1‑93 (MQ=255)

                                           ttttttCTCTGCGCCAACAGCAGCGCGGCAGCGGCCTGTTCATCAGCGCCGAGTAAACTTCGCAGTAGCGAAATATCCGGTCCGCTTTTcccc                                              >  2:618058/1‑93 (MQ=255)

                                           ttttttCTCTGCGCCAACAGCAGCGCGGCAGCGGCCTGTTCATCAGCGCCGAGTAAACTTCGCAGTAGCGAAATATCCGGTCCGCTTTTAccc                                              <  2:5295691/93‑1 (MQ=255)

                                           ttttttCTCTGCGCCAACAGCAGCGCGGCAGCGGCCTGTTCATCAGCGCCGAGTAAACTTCGCAGTAGCGAAATATCCGGTCCGCTTTTAccc                                              >  1:507822/1‑93 (MQ=255)

                                             ttttCTCTGCGCCAACATCAGTGCGGGAGCTGGCTGTGCATCAGCCCCGCGTAAACTTCGCAGTAGCGGAATATTCTGTCCGCTTTTTCCCTg                                            >  2:5518209/1‑93 (MQ=255)

                                             ttttCTCTGCGCCAACAGCAGCGCGGCAGCGGCCTGTTCATCAGCGCCGAGTAAACTTCGCAGTAGCGAAATATCCGGTCCGCTTTTACCCTg                                            <  1:4106129/93‑1 (MQ=255)

                                              tttCTCTGCGCCAACAGCAGCGCGGCAGCGGCCTGTTCATCAGCGCCGAGTAAACTTCGCAGTAGCGAAATATCCGGTCCGCTTTTACCCTGa                                           <  2:6060310/93‑1 (MQ=255)

                                              tttCTCTGCGCCAACAGCAGCGCGGCAGCGGCCTGTTCATCAGCGCCGAGTAAACTTCGCAGTAGCGAAATATCCGGTCCGCTTTTACCCTGa                                           <  2:2229564/93‑1 (MQ=255)

                                               ttCTCTGCGCCAACAGCAGCGCGGCAGCGGCCTGTTCATCAGCGCCGAGTAGACTTCGCAGTAGCGAAATATCCGGTCCGCTTTTACCCTGAg                                          <  1:2839461/93‑1 (MQ=255)

                                               ttCTCTGCGCCAACAGCAGCGCGGCAGCGGCCTGTTCATCAGCGCCGAGTAAACTTCGCAGTAGCGAAATATCCGGTCCGCTTTTACCCTGAg                                          <  2:897435/93‑1 (MQ=255)

                                                tctcTGCGCCAACAGCAGCGCGGCAGCGTCCTGTTCATCAGCGCCTAGTAAACTTCGCAGTAGCGAAATATCCGGTCCGCTTTTACCCTGAgc                                         <  1:3119805/93‑1 (MQ=255)

                                                 ctctGCGCCAACAGCAGCGCGGCAGCGGCCTGTTCATCAGCGCCGAGTAAACTTCGCAGTAGCGAAATATCCGGTCCGCTTTTACCCTGAgcc                                        <  1:5402809/93‑1 (MQ=255)

                                                  tctGCGCCAACAGCAGCGCGGCAGCGGCCTGTTCATCAGCGCCGAGTAAACTTCGCAGTAGCGAAATATCCGGTCCGCTTTTACCCTGAgccg                                       >  1:6417082/1‑93 (MQ=255)

                                                   ctGCGCCAACAGCAGCGCGGCAGCGGCCTGTTCATCAGCGCCGAGTAAACTTCGCAGTAGCGAAATATCCGGTCCGCTTTTACCCTGAgccgc                                      >  1:4196856/1‑93 (MQ=255)

                                                   ctGCGCCAACAGCAGCGCGGCAGCGGCCTGTTCATCAGCGCCGAGTAAACTTCGCAGTAGCGAAATATCCGGTCCGCTTTTACCCTGAgccgc                                      <  2:1432769/93‑1 (MQ=255)

                                                   ctGCGCCAACAGCAGCGCGGCAGCGGCCTGTTCATCAGCGCCGAGTAAACTTCGCAGTAGCGAAATATCCGGTCCGCTTTTACCCTGAgccgc                                      <  1:145750/93‑1 (MQ=255)

                                                    tGCGCCAACAGCAGCGCGGCAGCGGCCTGTTCATCAGCGCCGAGTAAACTTCGCAGTAGCGAAATATCCGGTCCGCTTTTACCCTGAgccgcc                                     <  1:733969/93‑1 (MQ=255)

                                                       gcCAACAGCAGCGCGGCAGCGGCCTGTTCATCAGCGCCGAGTAAACTTCGCAGTAGCGAAATATCCGGTCCGCTTTTACCCTGAgccgccgcc                                  <  2:4663228/93‑1 (MQ=255)

                                                        ccAACAGCAGCGCGGCAGCGGCCTGTTCATCAGCGCCGAGTAAACTTCGCAGTAGCGAAATATCCGGTCCGCTTTTACCCTGAGCCGCCGCCt                                 >  2:4483351/1‑93 (MQ=255)

                                                        ccAACAGCAGCGCGGCAGCGGCCTGTTCATCAGCGCCGAGTAAACTTCGCAGTAGCGAAATATCCGGTCCGCTTTTACCCTGAGCCGCCGCCt                                 >  1:1947506/1‑93 (MQ=255)

                                                         cAACAGCAGCGCGGCAGCGGCCTGTTCATCAGCGCCGAGTAAACTTCGCAGTAGCGAAATATCCGGTCCGCTTTTACCCTGAGCCGCCGCCTg                                >  1:1087399/1‑93 (MQ=255)

                                                           aaagcagcGCGGCAGCGGCCTGTTCATCAGCGCCGCGTCAACTTCCCAGTAGCGAAATATCCCGTCCGCTTTTATCCTGAGCCGCCGCCTGcc                              >  2:2916907/3‑93 (MQ=255)

                                                           aCAGCAGCGCGGCAGCGGCCTGTTCATCAGCGCCGAGTAAACTTCGCAGTAGCGAAATATCCGGTCCGCTTTTACCCTGAGCCGCCGCCTGcc                              <  2:2619282/93‑1 (MQ=255)

                                                           aCAGCAGCGCGGCAGCGGCCTGTTCATCAGCGCCGAGTAAACTTCGCAGTAGCGAAATATCCGGTCCGCTTTTACCCTGAGCCGCCGCCTGcc                              >  2:3965595/1‑93 (MQ=255)

                                                             agcagcGCGGCAGCGGCCTGTTCATCAGCGCCGAGTAAACTTCGCAGTAGCGAAATATCCGGTCCGCTTTTACCCTGAGCCGCCGCCTGCCAc                            <  2:4878255/93‑1 (MQ=255)

                                                              gcagcGCGGCAGCGGCCTGTTCATCAGCGCCGAGTAAACTTCGCAGTAGCGAAATATCCGGTCCGCTTTTACCCTGAGCCGCCGCCTGCCAct                           >  2:2796531/1‑93 (MQ=255)

                                                                agcGCGGCAGCGGCCTGTTCATCAGCGCCGAGTAAACTTCGCAGTAGCGAAATATCCGGTCCGCTTTTACCCTGAGCCGCCGCCTGCCActct                         >  2:3146221/1‑93 (MQ=255)

                                                                agcGCGGCAGCGGCCTGTTCATCAGCGCCGAGTAAACTTCGCAGTAGCAAAATATCCGGTCCGTTTTTACCCTGAGCCGCCGCCTGCCActct                         <  2:1886798/93‑1 (MQ=255)

                                                                 gcgcGGCAGCGGCCTGTTCATCAGCGCCGAGTAAACTTCGCAGTAGCGAAATATCCGGTCCGCTTTTACCCTGAGCCGCCGCCTGCCACTCtt                        >  1:3572392/1‑93 (MQ=255)

                                                                   gcggcggcgGCCTGTTCATCGGCGCCGAGTAAACTTCGCAGTAGCAAAATACCCGGTCCGCTTTTACCCTGAGCCGCCGCCTGCCACTCtttt                      <  2:5350523/93‑1 (MQ=255)

                                                                    cgGCAGCGGCCTGTTCATCAGCGCCGAGTAAACTTCGCAGTAGCGAAATATCCGGTCCGCTTTTCCCCTGAGCCGCCGCCTGCCACTCttttt                     <  1:4541543/93‑1 (MQ=255)

                                                                          cGGCCTGTCCATCAGCGCCGAGTAACCTTCGCAGTAGCGAAATATCCGGTCCGCTTTTCCCCTGAGCCGCCGCCTGCCACTCTTTTTCCGCCa               <  2:5663996/93‑1 (MQ=255)

                                                                             ccTGTTCATCAGCGCCGAGTAAACTTCGCAGTAGCGAAATATCCGGTCCGCTTTTACCCTGAGCCGCCGCCTGCCACTCTTTTTCCGCCAGCa            >  1:6079319/1‑93 (MQ=255)

                                                                              cTGTTCATCAGCGCCGAGTAAACTTCGCAGTAGCGAAATATCCGGTCCGCTTTTACCCTGAGCCGCCGCCTGCCACTCTTTTTCCGCCAGCAt           >  2:687008/1‑93 (MQ=255)

                                                                               tGTTCATCAGCGCCGAGTAAACTTCGCAGTAGCGAAATATCCGGTCCGCTTTTACCCTGAGCCGCCGCCTGCCACTCTTTTTCCGCCAGCAtt          >  1:3725358/1‑93 (MQ=255)

                                                                               tGTTCATCAGCGCCGAGTAAACTTCGCAGTAGCGAAATATCCGGTCCGCTTTTACCCTGAGCCGCCGCCTGCCACTCTTTTTCCGCCAGCAtt          >  1:6112623/1‑93 (MQ=255)

                                                                                gTTCATCAGCGCCGAGTAACCTTCGCAGTAGCGAAATACCCGGTCCGCTTTTCCCCTGAGCCCCCGCCTGCCACTCTTTTTCCGCCAGCATTc         <  2:5903754/93‑1 (MQ=255)

                                                                                 ttgatcaGCCCCGAGTAACATCCGCAGTAGCGAAATATCCGGTCCGTTTTTCCCCAAAGCCGCCGCCTGCCTCTCTTTTTCCGCCAGCATTcc        <  2:644130/90‑1 (MQ=255)

                                                                                     tcaGCGCCGAGTAAACTTCGCAGTAGCGAAATATCCGGTCCGCTTTTACCCTGAGCCGCCGCCTGCCACTCTTTTTCCGCCAGCATTCCGGCa    >  2:3815009/1‑93 (MQ=255)

                                                                                     tcaGCGCCCAGTAAACTTCGCAGTAGCGAAATATCCGGTCCGCTTTTACCCTGAGCCGCCGCCTGCCACTCTTTTTCCGCCAGCATTCCGGCa    >  2:6328827/1‑93 (MQ=255)

                                                                                      caGCGCCGAGTAAACTTCGCAGTAGCGAAATATCCGGTCCGCTTTTACCCTGAGCCGCCGCCTGCCACTCTTTTTCCGCCAGCATTCCGGCAt   >  2:3260631/1‑93 (MQ=255)

                                                                                       aGCGCCGAGTAAACTTCGCAGTAGCGAAATATCCGGTCCTCTTTGACCCTGAGCCGCCGCCTGCCAGTCTTTTTCCGCCAGCACTCCGGCATc  >  2:3697379/1‑93 (MQ=255)

                                                                                       aGCCCCAAGAAAACTTCGAAGTACCAAAAGATCGGGTCCGTTTTTCCCCGGAGCCGCCGCCTGCCACTCTTTTTCCGCCAGCATTCCGGCATc  <  2:1018586/93‑1 (MQ=255)

TTCATCCCCCATGCTCAACGCGGTTAAAGACATCGGCGTACCGTTTTTTCTCTGCGCCAACAGCAGCGCGGCAGCGGCCTGTTCATCAGCACCGAGTAAACTTCGCAGTAGCGAAATATCCGGTCCGCTTTTACCCTGAGCCGCCGCCTGCCACTCTTTTTCCGCCAGCATTCCGGCATC  >  NC_000913/2894366‑2894545

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 17 ≤` `ATCG/ATCG` `< 26 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

BRESEQ :: bam2aln output

CCGTTCATCCCCCATGCTCAACGCGGTTAAAGACATCGGCGTACCGTTTTTTCTCTGCGCCAACAGCAGCGCGGCAGCGGCCTGTTCATCAGCACCGAGTAAACTTCGCAGTAGCGAAATATCCGGTCCGCTTTTACCCTGAGCCGCCGCCTGCCACTCTTTTTCCGCCAGCATTCCGGCATCCGG  >  NC_000913/2894363‑2894548

CCGTTCATCCCCCATGCTCAACGCGGTTAAAGACATCGGCGTACCGTTTTTTCTCTGCGCCAACAGCAGCGCGGCAGCGGCCTGGTCATCAGCG                                                                                              >  SRR4302537.3415687/1‑94 (MQ=60)

 CGTTCATCCCCCATGCTCAACGCGGTTAAAGACATCGGCGTACCGTTTTTTCTCTGCGCCAACAGCAGCGCGGCAGCGGCCTGTTCATCAGCGC                                                                                             >  SRR4302537.3540412/1‑94 (MQ=60)

  GTTCATCCCCCATGCTCAACGCGGTTAAAGACATCGGCGTACCGTTTTTTCTCTGCGCCAACAGCAGCGCGGCAGCGGCCTGTTCATCAGCGCC                                                                                            <  SRR4302537.2439385/94‑1 (MQ=60)

  GTTCATCCCCCATGCTCAACGCGGTTAAAGACATCGGCGTACCGTTTTTTCTCTGCGCCAACAGCAGCGCGGCAGCGGCCTGTTCATCAGCGCC                                                                                            <  SRR4302537.3797303/94‑1 (MQ=60)

   TTCATCCCCCATGCTCAACGCGGTTAAAGACATCGGCGTACCGTTTTTTCTCTGCGCCAACAGCAGCGCGGCAGCGGCCTGTTCATCAGCGCCG                                                                                           <  SRR4302537.1617876/94‑1 (MQ=60)

   TTCATCCCCCATGCTCAACGCGGTTAAAGACATCGGCGTACCGTTTTTTCTCTGCGCCAACAGCAGCGCGGCAGCGGCCTGTTCATCAGCGCCG                                                                                           <  SRR4302537.519736/94‑1 (MQ=60)

     CATCCCCCATGCTCAACGCGGTTAAAGACATCGGCGTACCGTTTTTTCTCTGCGCCAACAGCAGCGCGGCAGCGGCCTGTTCATCAGCGCCGAG                                                                                         <  SRR4302537.480912/94‑1 (MQ=60)

     CATCCCCCATGCTCAACGCGGTTAAAGACATCGGCGTACCGTTTTTTCTCTGCGCCAACAGCAGCGCGGCAGCGGCCTGTTCATCAGCGCCGAG                                                                                         <  SRR4302537.4162834/94‑1 (MQ=60)

      ATCCCCCATGCTCAACGCGGTTAAAGACATCGGCGTACCGTTTTTTCTCTGCGCCAACAGCAGCGCGGCAGCGGCCTGTTCATCAGCGCCGAGT                                                                                        <  SRR4302537.4477884/94‑1 (MQ=60)

      ATCCCCCATGCTCAACGCGGTTAAAGACATCGGCGTACCGTTTTTTCTCTGCGCCAACAGCAGCGCGGCAGCGGCCTGTTCATCAGCGCCGAGT                                                                                        <  SRR4302537.5585189/94‑1 (MQ=60)

       TCCCCCATGCTCAACGCGGTTAAAGACATCGGCGTACCGTTTTTTCTCTGCGCCAACAGCAGCGCGGCAGCGGCCTGTTCATCAGCGCCGAGTA                                                                                       <  SRR4302537.4771662/94‑1 (MQ=60)

       TCCCCCATGCTCAACGCGGTTAAAGACATCGGCGTACCGTTTTTTCTCTGCGCCAACAGCAGCGCGGCAGCGGCCTGTTCATCAGCGCCGAGTA                                                                                       <  SRR4302537.142469/94‑1 (MQ=60)

        CCCCCATGCTCAACGCGGTTAAAGACATCGGCGTACCGTTTTTTCTCTGCGCCAACAGCAGCGCGGCAGAGGCCTGCTCATCAGCGGCGAGTAA                                                                                      >  SRR4302537.3827846/1‑94 (MQ=60)

         CCCCATGCTCAACGCGGTTAAAGACATCGGCGTACCGTTTTTTCTCTGCGCCAACAGCAGCGCGGCAGCGGCCTGTTCATCAGCGCCGAGTAAA                                                                                     >  SRR4302537.694526/1‑94 (MQ=60)

          CCCATGCTCAACGCGGTTAAAGACATCGGCGTACCGTTTTTTCTCTGCGCCAACAGCAGCGCGGCAGCGGCCTGTTCATCAGCGCCGAGTAAAC                                                                                    <  SRR4302537.217629/94‑1 (MQ=60)

          CCCATGCTCAACGCGGTTAAAGACATCGGCGTACCGTTTTTTCTCTGCGCCAACAGCAGCGCGGCAGCGGCCTGTTCATCAGCGCCGAGTAAAC                                                                                    >  SRR4302537.4546959/1‑94 (MQ=60)

           CCATGCTCAACGCGGTTAAAGACATCGGCGTACCGTTTTTTCTCTGCGCCAACAGCAGCGCGGCAGCGGCCTGTTCATCAGCGCCGAGTAAACT                                                                                   <  SRR4302537.5548879/94‑1 (MQ=60)

            CATGCTCAACGCGGTTAAAGACATCGGCGTACCGTTTTTTCTCTGCGCCAACAGCAGCGCGGCAGCGGCCTGTTCATCAGCGCCGAGTAAACTT                                                                                  >  SRR4302537.1945195/1‑94 (MQ=60)

             ATGCTCAACGCGGTTAAAGACATCGGCGTACCGTTTTTTCTCTGCGCCAACAGCAGCGCGGCAGCGGCCTGTTCATCAGCGCCGAGTAAACTTC                                                                                 >  SRR4302537.2137075/1‑94 (MQ=60)

             ATGCTCAACGCGGTTAAAGACATCGGCGTACCGTTTTTTCTCTGCGCCAACAGCAGCGCGGCAGCGGCCTGTTCATCAGCGCCGAGTAAACTTC                                                                                 >  SRR4302537.1864286/1‑94 (MQ=60)

              TGCTCAACGCGGTTAAAGACATCGGCGTACCGTTTTTTCTCTGCGCCAACAGCAGCGCGGCAGCGGCCTGTTCATCAGCGCCGAGTAAACTTCG                                                                                <  SRR4302537.2290709/94‑1 (MQ=60)

              TGCTCAACGCGGTTAAAGACATCGGCGTACCGTTTTTTCTCTGCGCCAACAGCAGCGCGGCAGCGGCCTGTTCATCAGCGCCGAGTAAACTTCG                                                                                <  SRR4302537.907339/94‑1 (MQ=60)

              TGCTCAACGCGGTTAAAGACATCGGCGTACCGTTTTTTCTCTGCGCCAACAGCAGCGCGGCAGCGGCCTGTTCATCAGCGCCGAGTAAACTTCG                                                                                >  SRR4302537.324928/1‑94 (MQ=60)

               GCTCAACGCGGTTAAAGACATCGGCGTACCGTTTTTTCTCTGCGCCAACAGCAGCGCGGCAGCGGCCTGTTCATCAGCGCCGAGTAAACTTCGC                                                                               >  SRR4302537.4271323/1‑94 (MQ=60)

               GCTCAACGCGGTTAAAGACATCGGCGTACCGTTTTTTCTCTGCGCCAACAGCAGCGCGGCAGCGGCCTGTTCATCAGCGCCGAGTAAACTTCGC                                                                               <  SRR4302537.715933/94‑1 (MQ=60)

                CTCAACGCGGTTAAAGACATCGGCGTACCGTTTTTTCTCTGCGCCAACAGCAGCGCGGCAGCGGCCTGTTCATCAGCGCCGAGTAAACTTCGCA                                                                              >  SRR4302537.1579090/1‑94 (MQ=60)

                CTCAACGCGGTTAAAGACATCGGCGTACCGTTTTTTCTCTGCGCCAACAGCAGCGCGGCAGCGGCCTGTTCATCAGCGCCGAGTAAACTTCGCA                                                                              >  SRR4302537.2094333/1‑94 (MQ=60)

                CTCAACGCGGTTAAAGACATCGGCGTACCGTTTTTTCTCTGCGCCAACAGCAGCGCGGCAGCGGCCTGTTCATCAGCGCCGAGTAAACTTCGCA                                                                              <  SRR4302537.1533384/94‑1 (MQ=60)

                CTCAACGCGGTTAAAGACATCGGCGTACCGTTTTTTCTCTGCGCCAACAGCAGCGCGGCAGCGGCCTGTTCATCAGCGCCGAGTAAACTTCGCA                                                                              <  SRR4302537.2363245/94‑1 (MQ=60)

                  CAACGCGGTTAAAGACATCGGCGTACCGTTTTTTCTCTGCGCCAACAGCAGCGCGGCAGCGGCCTGTTCATCAGCGCCGAGTAAACTTCGCAGT                                                                            >  SRR4302537.1622226/1‑94 (MQ=60)

                  CAACGCGGTTAAAGACATCGGCGTACCGTTTTTTCTCTGCGCCAACAGCAGCGCGGCAGCGGCCTGTTCATCAGCGCCGAGTAAACTTCGCAGT                                                                            >  SRR4302537.653980/1‑94 (MQ=60)

                   AACGCGGTTAAAGACATCGGCGTACCGTTTTTTCTCTGCGCCAACAGCAGCGCGGCAGCGGCCTGTTCATCAGCGCCGAGTAAACTTCGCAGTA                                                                           >  SRR4302537.2796236/1‑94 (MQ=60)

                     CGCGGTTAAAGACATCGGCGTACCGTTTTTTCTCTGCGCCAACAGCAGCGCGGCAGCGGCCTGTTCATCAGCGCCGAGTAAACTTCGCAGTAGC                                                                         >  SRR4302537.787730/1‑94 (MQ=60)

                       CGGTTAAAGACATCGGCGTACCGTTTTTTCTCTGCGCCAACAGCAGCGCGGCAGCGGCCTGTTCATCAGCGCCGAGTAAACTTCGCAGTAGCGA                                                                       >  SRR4302537.6132923/1‑94 (MQ=60)

                        GGTTAAAGACATCGGCGTACCGTTTTTTCTCTGCGCCAACAGCAGCGCGGCAGCGGCCTGTTCATCAGCGCCGAGTAAACTTCGCAGTAGCGAA                                                                      >  SRR4302537.5132406/1‑94 (MQ=60)

                          TTAAAGACATCGGCGTACCGTTTTTTCTCTGCGCCAACAGCAGCGCGGCAGCGGCCTGTTCATCAGCGCCGAGTAAACTTCGCAGTAGCGAAAT                                                                    <  SRR4302537.6152048/94‑1 (MQ=60)

                             AAGACATCGGCGTACCGTTTTTTCTCTGCGCCAACAGCAGCGCGGCAGCGGCCTGTTCATCAGCGCCGAGTAAACTTCGCAGTAGCGAAATATC                                                                 >  SRR4302537.155967/1‑94 (MQ=60)

                             AAGACATCGGCGTACCGTTTTTTCTCCGCGCCAACAGCAGCGCGGCAGCGGCCTGTTCATCAGCACCGAGTAAACTTCGCAGTAGCGAAATATC                                                                 >  SRR4302537.1541632/1‑94 (MQ=60)

                               GACATCGGCGTACCGTTTTTTCTCTGCGCCAACAGCAGCGCGGCAGCGGCCTGTTCATCAGCGCCGAGTAAACTTCGCAGTAGCGAAATATCCG                                                               >  SRR4302537.4782415/1‑94 (MQ=60)

                               GACATCGGCGTACCGTTTTTTCTCTGCGCCAACAGCAGCGCGGCAGCGGCCTGTTCATCAGCGCCGAGTAAACTTCGCAGTAGCGAAATATCCG                                                               >  SRR4302537.4070449/1‑94 (MQ=60)

                               GACATCGGCGTACCGTTTTTTCTCTGCGCCAACAGCAGCGCGGCAGCGGCCTGTTCATCAGCGCCGAGTAAACTTCGCAGTAGCGAAATATCCG                                                               <  SRR4302537.1539663/94‑1 (MQ=60)

                               GACATCGGCGTACCGTTTTTTCTCTGCGCCAACAGCAGCGCGGCAGCGGCCTGTTCATCAGCGCCGAGTAAACTTCGCAGTAGCGAAATATCCG                                                               <  SRR4302537.5253091/94‑1 (MQ=60)

                               GACATCGGCGTACCGTTTTTTCTCTGCGCCAACAGCAGCGCGGCAGCGGCCTGTTCATCAGCGCCGAGTAAACTTCGCAGTAGCGAAATATCCG                                                               <  SRR4302537.6092981/94‑1 (MQ=60)

                                ACATCGGCGTACCGTTTTTTCTCTGCGCCAACAGCAGCGCGGCAGCGGCCTGTTCATCAGCGGCGAGTAAAGCTCGCAGTAGCGCAATATACGG                                                              >  SRR4302537.4486023/1‑94 (MQ=60)

                                ACATCGGCGTACCGTTTTTTCTCTGCGCCAACAGCAGCGCGGCAGCGGCCTGTTCATCAGCGCCGAGTAAACTTCGCAGTAGCGAAATATCCGG                                                              >  SRR4302537.1609877/1‑94 (MQ=60)

                                 CATCGGCGTACCGTTTTTTCTCTGCGCCAACAGCAGCGCGGCAGCGGCCTGTTCATCAGCGCCGAGTAAACTTCGCAGTAGCGAAATATCCGGT                                                             <  SRR4302537.1255184/94‑1 (MQ=60)

                                 CATCGGCGTACCGTTTTTTCTCTGCGCCAACAGCAGCGCGGCAGCGGCCTGTTCATCAGCGCCGAGTAAACTTCGCAGTAGCGAAATATCCGGT                                                             <  SRR4302537.3204406/94‑1 (MQ=60)

                                 CATCGGCGTACCGTTTTTTCTCTGCGCCAACAGCAGCGCGGCAGCGGCCTGTTCATCAGCGCCGAGTAAACTTCGCAGTAGCGAAATATCCGGT                                                             >  SRR4302537.6047989/1‑94 (MQ=60)

                                  ATCGGCGTACCGTTTTTTCTCTGCGCCAACAGCAGCGCGGCCGCGGCCTGTTCCTCAGCGCCGAGCAAACTTCCCAGTAGCGCAATATCCGGGC                                                            >  SRR4302537.5524919/1‑94 (MQ=60)

                                  ATCGGCGTACCGTTTTTTCTCTGCGCCAACAGCAGCGCGGCAGCGGCCTGTTCATCAGCGCCGAGTAAACTTCGCAGTAGCGAAATATCCGGTC                                                            >  SRR4302537.2141603/1‑94 (MQ=60)

                                   TCGGCGTACCGTTTTTTCTCTGCGCCAACAGCAGCGCGGCAGCGGCCTGTTCATCAGCGCCGAGTAAACTTCGCAGTAGCGAAATATCCGGTCC                                                           <  SRR4302537.5612349/94‑1 (MQ=60)

                                      GCGTACCGTTTTTTCTCTGCGCCAACAGCAGCGCGGCAGCGGCCTGTTCATCAGCGCCGAGTAAACTTCGCAGTAGCGAAATATCCGGTCCGCT                                                        >  SRR4302537.32951/1‑94 (MQ=60)

                                      GCGTACCGTTTTTTCTCTGCGCCAACAGCAGCGCGGCAGCGGCCTGTTCATCAGCGCCGAGTAAACTTCGCAGTAGCGAAATATCCGGTCCGCT                                                        <  SRR4302537.5933671/94‑1 (MQ=60)

                                       CGTACCGTTTTTTCTCTGCGCCAACAGCAGCGCGGCAGCGGCCTGTTCATCAGCGCCGAGTAAACTTCGCAGTAGCGAAATATCCGGTCCGCTT                                                       <  SRR4302537.3660105/94‑1 (MQ=60)

                                         TACCGTTTTTTCTCTGCGCCAACAGCAGCGCGGCAGCGGCCTGTTCATCAGCGCCGAGTAAACTTCGCAGTAGCGAAATATCCGGTCCGCTTTT                                                     <  SRR4302537.4089243/94‑1 (MQ=60)

                                         TACCGTTTTTTCTCTGCGCCAACAGCAGCGCGGCAGCGGCCTGTTCATCAGCGCCGAGTAAACTTCGCAGTAGCGAAATATCCGGTCCGCGTTT                                                     >  SRR4302537.3792806/1‑94 (MQ=60)

                                           CCGTTTTTTCTCTGCGCCAACAGCAGCGCGGCAGCGGCCTGTTCATCAGCGCCGAGTAAACTTCGCAGTAGCGAAATATCCGGTCCGCTTTTAC                                                   <  SRR4302537.5191364/94‑1 (MQ=60)

                                           CCGTTTTTTCTCTGCGCCAACAGCAGCGCGGCAGCGGCCTGTTCATCAGCGCCGAGTAAACTTCGCAGTAGCGAAATATCCGGTCCGCTTTTAC                                                   >  SRR4302537.2466564/1‑94 (MQ=60)

                                            CGTTTTTTCTCTGCGCCAACAGCAGCGCGGCAGCGGCCTGTTCATCAGCGCCGAGTAAACTTCGCAGTAGCGAAATATCCGGTCCGCTTTTACC                                                  <  SRR4302537.1590077/94‑1 (MQ=60)

                                            CGTTTTTTCTCTGCGCCAACAGCAGCGCGGCAGCGGCCTGTTCATCAGCGCCGAGTAAACTTCGCAGTAGCGAAATATCCGGTCCGCTTTTACC                                                  >  SRR4302537.3154183/1‑94 (MQ=60)

                                             GTTTTTTCTCTGCGCCAACAGCAGCGCGGCAGCGGCCTGTTCATCAGCGCCGAGTAAACTTCGCAGTAGCGAAATATCCGGTCCGCTTTTTCCC                                                 >  SRR4302537.1161402/1‑94 (MQ=60)

                                             GTTTTTTCTCTGCGCCAACAGCAGCGCGGCAGCGGCCTGTTCATCAGCGCCGAGTAAACTTCGCAGTAGCGAAATATCCGGTCCGCTTTTACCC                                                 >  SRR4302537.511307/1‑94 (MQ=60)

                                             GTTTTTTCTCTGCGCCAACAGCAGCGCGGCAGCGGCCTGTTCATCAGCGCCGAGTAAACTTCGCAGTAGCGAAATATCCGGTCCGCTTTTACCC                                                 >  SRR4302537.1576242/1‑94 (MQ=60)

                                             GTTTTTTCTCTGCGCCAACAGCAGCGCGGCAGCGGCCTGTTCATCAGCGCCGAGTAAACTTCGCAGTAGCGAAATATCCGGTCCGCTTTTACCC                                                 >  SRR4302537.6014716/1‑94 (MQ=60)

                                                TTTTCTCTGCGCCAACAGCAGCGCGGCAGCGGCCTGTTCATCAGCGCCGAGTAAACTTCGCAGTAGCGAAATATCCGGTCCGCTTTTACCCTGA                                              <  SRR4302537.4134814/94‑1 (MQ=60)

                                                 TTTCTCTGCGCCAACAGCAGCGCGGCAGCGGCCTGTTCATCAGCGCCGAGTAAACTTCGCAGTAGCGAAATATCCGGTCCGCTTTTACCCTGAG                                             <  SRR4302537.4619808/94‑1 (MQ=60)

                                                  TTCTCTGCGCCAACAGCAGCGCGGCAGCGGCTTGTTCATCAGCGCCGAGTAAACTTCGCAGTAGCGAAATATCCGGTCCGCTTTTACCCTGAGC                                            <  SRR4302537.2859333/94‑1 (MQ=60)

                                                  TTCTCTGCGCCAACAGCAGCGCGGCAGCGGCCTGTTCATCAGCGCCGAGTAGACTTCGCAGTAGCGAAATATCCGGTCCGCTTTTACCCTGAGC                                            <  SRR4302537.2859332/94‑1 (MQ=60)

                                                   TCTCTGCGCCAACAGCAGCGCGGCAGCGTCCTGTTCATCAGCGCCTAGTAAACTTCGCAGTAGCGAAATATCCGGTCCGCTTTTACCCTGAGCC                                           <  SRR4302537.3141614/94‑1 (MQ=60)

                                                    CTCTGCGCCAACAGCAGCGCGGCAGCGGCCTGTTCATCAGCGCCGAGTAAACTTCGCAGTAGCGAAATATCCGGTCCGCTTTTACCCTGAGCCG                                          <  SRR4302537.5439676/94‑1 (MQ=60)

                                                    CTCTGCGCCAACAGCAGCGCGGCAGCGGCCTGTTCATCAGCGCCGAGTAAACTTCGCAGTAGCGAAATATCCGGTCCGCTTTTACCCTGAGCCG                                          >  SRR4302537.6460721/1‑94 (MQ=60)

                                                     TCTGCGCCAACAGCAGCGCGGCAGCGGCCTGTTCATCAGCGCCGAGTAAACTTCGCAGTAGCGAAATATCCGGTCCGCTTTTACCCTGAGCCGC                                         >  SRR4302537.4226164/1‑94 (MQ=60)

                                                     TCTGCGCCAACAGCAGCGCGGCAGCGGCCTGTTCATCAGCGCCGAGTAAACTTCGCAGTAGCGAAATATCCGGTCCGCTTTTACCCTGAGCCGC                                         <  SRR4302537.5296511/94‑1 (MQ=60)

                                                      CTGCGCCAACAGCAGCGCGGCAGCGGCCTGTTCATCAGCGCCGAGTAAACTTCGCAGTAGCGAAATATCCGGTCCGCTTTTACCCTGAGCCGCC                                        <  SRR4302537.146789/94‑1 (MQ=60)

                                                      CTGCGCCAACAGCAGCGCGGCAGCGGCCTGTTCATCAGCGCCGAGTAAACTTCGCAGTAGCGAAATATCCGGTCCGCTTTTACCCTGAGCCGCC                                        <  SRR4302537.1660464/94‑1 (MQ=60)

                                                       TGCGCCAACAGCAGCGCGGCAGCGGCCTGTTCATCAGCGCCGAGTAAACTTCGCAGTAGCGAAATATCCGGTCCGCTTTTACCCTGAGCCGCCG                                       <  SRR4302537.738976/94‑1 (MQ=60)

                                                          GCCAACAGCAGCGCGGCAGCGGCCTGTTCATCAGCGCCGAGTAAACTTCGCAGTAGCGAAATATCCGGTCCGCTTTTACCCTGAGCCGCCGCCT                                    >  SRR4302537.1961049/1‑94 (MQ=60)

                                                          GCCAACAGCAGCGCGGCAGCGGCCTGTTCATCAGCACCGAGTAAACTTCGCAGTAGCGAAATATCCGGTCCGCTTTTACCCTGAGCCGCCGCCT                                    <  SRR4302537.1339602/94‑1 (MQ=60)

                                                           CCAACAGCAGCGCGGCAGCGGCCTGTTCATCAGCGCCGAGTAAACTTCGCAGTAGCGAAATATCCGGTCCGCTTTTACCCTGAGCCGCCGCCTG                                   >  SRR4302537.1094905/1‑94 (MQ=60)

                                                            CAACAGCAGCGCGGCAGCGGCCTGTTCATCAGCGCCGAGTAAACTTCGCAGTAGCGAAATATCCGGTCCGCTTTTACCCTGAGCCGCCGCCTGC                                  >  SRR4302537.718114/1‑94 (MQ=60)

                                                            CAACAGCAGCGCGGCAGCGGCCTGTTCATCAGCGCCGAGTAAACTTCGCAGTAGCGAAATATCCGGTCCGCTTTTACCCTGAGCCGCCGCCTGC                                  >  SRR4302537.2093286/1‑94 (MQ=60)

                                                             AACAGCAGCGCGGCAGCGGCCTGTTCATCAGCGCCGAGTAAACTTCGCAGTAGCGAAATATCCGGTCCGCTTTTACCCTGAGCCGCCGCCTGCC                                 <  SRR4302537.2746769/94‑1 (MQ=60)

                                                               CAGCAGCGCGGCAGCGGCCTGTTCATCAGCGCCGAGTAAACTTCGCAGTAGCGAAATATCCGGTCCGCTTTTACCCTGAGCCGCCGCCTGCCAC                               >  SRR4302537.1297990/1‑94 (MQ=60)

                                                                   AGCGCGGCAGCGGCCTGTTCATCAGCGCCGAGTAAACTTCGCAGTAGCGAAATATCCGGTCCGCTTTTACCCTGAGCCGCCGCCTGCCACTCTT                           >  SRR4302537.3597582/1‑94 (MQ=60)

                                                                   AGCGCGGCAGCGGCCTGTTCATCAGCGCCGAGTAAACTTCGCAGTAGCGAAATATCCGGTCCGCTTTTACCCTGAGCCGCCGCCTGCCACTCTT                           >  SRR4302537.5763341/1‑94 (MQ=60)

                                                                    GCGCGGCAGCGGCCTGTTCACCAGCGCCGAGTAAACTTCGCAGTAGCGAAATATCCGGTCCTTTTTTACCCTGACCCGCCGCCTGCCACTCTTT                          <  SRR4302537.5270647/94‑1 (MQ=60)

                                                                     CGCGGCAGCGGCCTGTTCATCAGCGCCGAGTAAACTTCGCAGTAGCGAAATATCCGGTCCGCTTTTACCCTGAGCCGCCGCCTGCCACTCTTTT                         <  SRR4302537.4016655/94‑1 (MQ=60)

                                                                      GCGGCAGCGGCCTGTTCATCAGCGCCGAGTAAACTTCGCAGTAGCGAAATATCCGGTCCGCTTTTACCCTGAGCCGCCGCCTTCCACTCTTTTT                        <  SRR4302537.5341250/94‑1 (MQ=60)

                                                                       CGGCAGCGGCCTGTTCATCAGCGCCGAGTAAACTTCGCAGTAGCGAAATATCCGGTCCGCTTTTCCCCTGAGCCGCCGCCTGCCACTCTTTTTC                       <  SRR4302537.4572979/94‑1 (MQ=60)

                                                                       CGGCAGCGGCCTGTTCATCAGCGCCGAGTAAACTTCGCAGTAGCGAAATATCCGGTCCGCTTTTACCCTGAGCCGCCGCCTGCCACTCTTTTTC                       >  SRR4302537.2418096/1‑94 (MQ=60)

                                                                         GCAGCGGCCTGTTCATCAGCGCCGAGTAAACTTCGCAGTAGCGAAATATCCGGTCCGCTTTTCCCCTGAGCCGCCGCCTGCCACTCTTTTTCCG                     <  SRR4302537.14968/94‑1 (MQ=60)

                                                                          CAGCGGCCTGTTCATCAGCGCCGAGTAAACTTCGCAGTAGCGAAATATCCGGTCCGCTTTTACCCTGAGCCGCCGCCTGCCACTCTTTTTCCGC                    >  SRR4302537.2372708/1‑94 (MQ=60)

                                                                           GGCGGCCTGTTCATCAGCGCCGAGAAAACTTCGCAGTAGCGAAATATCCGGCCCGTTTTTCCCCTGAGCCGCCGCCTGCCACTCTTTTTCCGCC                   <  SRR4302537.5049569/94‑1 (MQ=60)

                                                                           AGCGGCCTGTTCATCAGCGCCGAGTAAACTTCGCAGTAGCGAAATATCCGGTCCGCTTTTACCCTGAGCCGCCGCCTGCCACTCTTTTTCCGCC                   >  SRR4302537.993773/1‑94 (MQ=60)

                                                                             CGCCCTTTTCATCAGCGCCGAGTAACCTCCGCAGTAGCGAAATATCCGGTCCGCTTTTCCCCTGAGCCGCCGCCTGCCACTCTTTTTCCGCCAG                 <  SRR4302537.1556766/94‑1 (MQ=60)

                                                                             CGCCCTGTCCATCAGCGCCGATAAAACTTCGCAGTAGCGAAATATCCGGTCCGCTTTTCCCCTGAGCCGCCGCCTGCCACTCTTTTTCCGCCAG                 <  SRR4302537.6166703/94‑1 (MQ=60)

                                                                               GCCTGTTCATCAGCGCCGAGTAAACTTCGCAGTAGCGAAATATCCGGTCCGCTTTTACCCTGAGCCGCCGCCTGCCACTCTTTTTCCGCCAGCA               >  SRR4302537.6121096/1‑94 (MQ=60)

                                                                                CCTGTTCATCAGCGCCGAGTAAACTTCGCAGTAGCGAAATATCCGGTCCGCTTTTCCCCTGAGCCGCCGCCTGCCACTCTTTTTCCGCCAGCAT              <  SRR4302537.1621578/94‑1 (MQ=60)

                                                                                 CTGTTCATCAGCGCCGAGTAAACTTCGCAGTAGCGAAATATCCGGTCCGCTTTTACCCTGAGCCGCCGCCTGCCACTCTTTTTCCGCCAGCATT             >  SRR4302537.3751532/1‑94 (MQ=60)

                                                                                 CTGTTCATCAGCGCCGAGTAAACTTCGCAGTAGCGAAATATCCGGTCCGCTTTTACCCTGAGCCGCCGCCTGCCACTCTTTTTCCGCCAGCATT             >  SRR4302537.6154631/1‑94 (MQ=60)

                                                                                 CTGTTCATCAGCGCCGAGTAAACTTCGCAGTAGCAAAATTTCCGGCCCGCTTTTCCCCTGAGCCGCCGCCTGCCACTCTTTTTCCGCCAGCATT             <  SRR4302537.4888408/94‑1 (MQ=60)

                                                                                  TGTTCATCAGCGCCGAGTAAACTTCGCAGTAGCGAAATATCCGGTCCGCTTTTACCCTGAGCCGCCGCCTGCCACTCTTTTTCCGCCAGCATTC            >  SRR4302537.3081190/1‑94 (MQ=60)

                                                                                   GTTCATCAGCGCCGAGTAAACTTCGCAGTAGCGAAATATCCGGTCCGCTTTTACCCTGAGCCGCCGCCTGCCACTCTTTTTCCGCCAGCATTCC           >  SRR4302537.777641/1‑94 (MQ=60)

                                                                                    TTCATCAGCGCGGAGTAAACTTCGCAGTAGCAAAATACCCGGTCCGTTTTTACCCTGAGCCGCCGCCTGCCACTCTTTTTCCGCCAGCATTCCG          <  SRR4302537.2386935/94‑1 (MQ=60)

                                                                                    TTCATCAGCGCCGAGTAAACTTCGCAGTAGCAAAATATCCGGTCCGCTTTTCCCCTGAGCCGCCGCCTGCCACTCTTTTTCCGCCAGCATTCCG          <  SRR4302537.4721788/94‑1 (MQ=60)

                                                                                    TTCATCAGCGCCGAGTAAACTTCGCAGTAGCAAAATATCCGGTCCGCTTTTACCCTGAGCCGCCGCCTGCCACTCTTTTTCCGCCAGCATTCCG          <  SRR4302537.2850413/94‑1 (MQ=60)

                                                                                    TTCATCAGCGCAGATAAAACTTCGCAGTAGCAAAATATCCGGTCCGCTTTTCCCCTGACCCGCCGCCTGCCACTCTTTTTCCGCCAGCATTCCG          <  SRR4302537.1424345/94‑1 (MQ=60)

                                                                                    TCCATCAGCGCCGATTAAACTTCGCAGTAGCGAAATGTCCGGTCCGCTTTTACCCTGAGCCGCCGCCTGCCACTCTTTTTCCGCCAGCATTCCG          <  SRR4302537.5753712/94‑1 (MQ=60)

                                                                                     TCATCAGGCCCGAGTAAACTTCGCAGTAGCAAAATATCCGGTCCGCTTTTCCCCGGAGCCGCCGCCTGCCACTCTTTTTCCGCCAGCATTCCGG         <  SRR4302537.1379642/94‑1 (MQ=60)

                                                                                     TCATCAGCGCCGAGTAAACTTCGCAGTAGCAAAATATCCGGTCCGCTTTTCCCCTGAGCCGCCGCCTGCCACTCTTTTTCCGCCAGCATTCCGG         <  SRR4302537.4061923/94‑1 (MQ=60)

                                                                                      CATCAGCGCCGAGTAATCTGCGCAGTAGCAAAATATCCGGTCCGTTTTTCCCCTGAGCCGCCGCCTGCCACTCTTTTTCCGCCAGCATTCCGGC        <  SRR4302537.304995/94‑1 (MQ=60)

                                                                                      CATCAGCGCCGAGTAAACTTCGCAGTAGCGAAATATCCGGTCCGCTTTTACCCTGAGCCGCCGCCTGCCACTCTTTTTCCGCCAGCATTCCGGC        >  SRR4302537.5690197/1‑94 (MQ=60)

                                                                                         CAGCGCCGAGTAAACTTCGCAGTAGCGAAATTTCCGGTCCGTTTTTACCCTGAGCCCCCGCCTGCCACTCTTTTTCCGCCAGCATTCCGGCATC     <  SRR4302537.1432260/94‑1 (MQ=60)

                                                                                            CGCCGAGTAAACTTCGCAGTAGCGAAATATCCGGTCCGCTTTTACCCTGAGCCGCCGCCTGCCACTCTTTTTCCGCCAGCATTCCGGCATCCGG  >  SRR4302537.3921673/1‑94 (MQ=60)

CCGTTCATCCCCCATGCTCAACGCGGTTAAAGACATCGGCGTACCGTTTTTTCTCTGCGCCAACAGCAGCGCGGCAGCGGCCTGTTCATCAGCACCGAGTAAACTTCGCAGTAGCGAAATATCCGGTCCGCTTTTACCCTGAGCCGCCGCCTGCCACTCTTTTTCCGCCAGCATTCCGGCATCCGG  >  NC_000913/2894363‑2894548

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 0 ≤` `ATCG/ATCG` `< 2 ≤` `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`