Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I2 R1 882 254.4 12957588 98.0% 12698436 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,025,676 +C coding (535/570 nt) ygfQ → hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,025,6751.C99.5% 705.4 / ‑4.0 202coding (534/570 nt)ygfQhypothetical protein
Reads supporting (aligned to +/- strand):  ref base . (0/1);  new base C (124/77);  total (124/78)
Fisher's exact test for biased strand distribution p-value = 3.86e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.96e-01

Only 100 of 218 total aligned reads displayed.

GCTCACGGTACAGGTATCGGTATCGGGCTGTTTCTGCTGCTGATTGCTGCTAACGGTGTGGGTATGGTTATCAAAAACCCGATTGAAGGCTTG‑C‑AGTGGCGCTCGGTGCGTTTACCTCCTTCCCGGTGATGATGAGCTTGCTGGGGCTGGCGGTCATCTTCGGCCTGGAGAAGTGTCGCG  >  NC_000913/3025583‑3025762
                                                                                             |                                                                                        
 cTCACGGTACAGGTATCGGTATCGGGCTGTTTCTGCTGCTGATTGCTGCTAACGGTGTGGGTATGGTTATCAAAAACCCGATTGAAGGCTTG‑c                                                                                         >  1:5081146/1‑93 (MQ=255)
 cTCACGGTACAGGTATCGGTATCGGGCTGTTTCTGCTGCTGATTGCTGCTAACGGTGTGGGTATGGTTATCAAAAACCCGATTGAAGGCTTG‑c                                                                                         <  2:107666/93‑1 (MQ=255)
  tCACGGTACAGGTATCGGTATCGGGCTGTTTCTGCTGCTGATTGCTGCTAACGGTGTGGGTATGGTTATCAAAAACCCGATTGAAGGCTTG‑cc                                                                                        >  2:2576709/1‑92 (MQ=255)
   cACGGTACAGGTATCGGTATCGGGCTGTTTCTGCTGCTGATTGCTGCTAACGGTGTGGGTATGGTTATCAAAAACCCGATTGAAGGCTTG‑cca                                                                                       >  2:4345009/1‑91 (MQ=255)
   cACGCTACAGGTATCGGTATCGGGCTGTTTCTGCTGCTGATTGCTGCTAACGGTGTGGGTATGGTTATCAAAAACCCGATTGAAGGCTTG‑cca                                                                                       <  1:2030979/93‑3 (MQ=255)
    aCGGTACAGGTATCGGTATCGGGCTGTTTCTGCTGCTGATTGCTGCTAACGGTGTGGGTATGGTTATCAAAAACCCGATTGAAGGCTTG‑ccag                                                                                      <  1:4306604/93‑4 (MQ=255)
    aCGGTACAGGTATCGGTATCGGGCTGTTTCTGCTGCTGATTGCTGCTAACGGTGTGGGTATGGTTATCAAAAACCCGATTGAAGGCTTG‑ccag                                                                                      >  2:1456057/1‑90 (MQ=255)
         aCAGGTATCGGTATCGGGCTGTTTCTGCTGCTGATTGCTGCTAACGGTGTGGGTATGGTTATCAAAAACCCGATTGAAGGCTTGCC‑AGTGgcg                                                                                 >  1:1259502/1‑93 (MQ=255)
         aCAGGTATCGGTATCGGGCTGTTTCTGCTGCTGATTGCTGCTAACGGTGTGGGTATGGTTATCAAAAACCCGATTGAAGGCTTGCC‑AGTGgcg                                                                                 >  2:1584837/1‑93 (MQ=255)
          cAGGTATCGGTATCGGGCTGTTTCTGCTGCTGATTGCTGCTAACGGTGTGGGTATGGTTATCAAAAACCCGATTGAAGGCTTGCC‑AGTGgcgc                                                                                <  2:6264037/93‑1 (MQ=255)
          cAGGTATCGGTATCGGGCTGTTTCTGCTGCTGATTGCTGCTAACGGTGTGGGTATGGTTATCAAAAACCCGATTGAAGGCTTGCC‑AGTGgcgc                                                                                >  2:5879065/1‑93 (MQ=255)
           aGGTATCGGTATCGGGCTGTTTCTGCTGCTGATTGCTGCTAACGGTGTGGGTATGGTTATCCAAAACCCGATTGAAGGCTTGCC‑AGTGGCGCt                                                                               >  2:1595357/1‑93 (MQ=255)
           aGGTATCGGTATCGGGCTGTTTCTGCTGCTGATTGCTGCTAACGGTGTGGGTATGGTTATCAAAAACCCGATTGAAGGCTTGCC‑AGTGGCGCt                                                                               <  1:5652199/93‑1 (MQ=255)
            ggtatcggtatcGGGCTGTTTCTGCTGCTGATTGCTGCTAACGGTGTGGGTATGGTTATCAAAAACCCGATTGAAGGCTTGCC‑AGTGGCGCTc                                                                              >  2:125519/1‑93 (MQ=255)
             gtatcggtatcgGGCTGTTTCTGCTGCTGATTGCTGCTAACGGTGTGGGTATGGTTATCAAAAACCCGATTGAAGGCTTGCC‑AGTGGCGCTCg                                                                             <  2:5731120/93‑1 (MQ=255)
             gtatcggtatcgGGCTGTTTCTGCTGCTGATTGCTGCTAACGGTGTGGGTATGGTTATCAAAAACCCGATTGAAGGCTTGCC‑AGTGGCGCTCg                                                                             >  1:95475/1‑93 (MQ=255)
              tatcggtatcggGCTGTTTCTGCTGCTGATTGCTGCTAACGGTGTGGGTATGGTTATCAAAAACCCGATTGAAGGCTTGCC‑AGTGGCGCTCgg                                                                            <  2:6301092/93‑1 (MQ=255)
              tatcggtatcggGCTGTTTCTGCTGCTGATTGCTGCTAACGGTGTGGGTATGGTTATCAAAAACCCGATTGAAGGCTTGCC‑AGTGGCGCTCgg                                                                            >  2:3427718/1‑93 (MQ=255)
                tcggtatcggGCTGTTTCTGCTGCTGATTGCTGCTAACGGTGTGGGTATGGTTATCAAAAACCCGATTGAAGGCTTGCC‑AGTGGCGCTCGGTg                                                                          <  1:3054784/93‑1 (MQ=255)
                tcggtatcggGCTGTTTCTGCTGCTGATTGCTGCTAACGGTGTGGGTATGGTTATCAAAAACCCGATTGAAGGCTTGCC‑AGTGGCGCTCGGTg                                                                          >  1:2692245/1‑93 (MQ=255)
                 cggtatcggGCTGTTTCTGCTGCTGATTGCTGCTAACGGTGTGGGTATGGTTATCAAAAACCCGATTGAAGGCTTGCC‑AGTGGCGCTCGGTGc                                                                         <  1:2834405/93‑1 (MQ=255)
                 cggtatcggGCTGTTTCTGCTGCTGATTGCTGCTAACGGTGTGGGTATGGTTATCAAAAACCCGATTGAAGGCTTGCC‑AGTGGCGCTCGGTGc                                                                         >  1:3571351/1‑93 (MQ=255)
                  ggtatcggGCTGGTTCTGCTGCTGATTGCTGCTAACGGTGTGGGTATGGTTATCAAAAACCCGATTGAAGGCTTGCC‑AGTGGCGCTCGGTGCg                                                                        <  1:4863999/93‑1 (MQ=255)
                   gtatcggGCTGTTTCTGCTGCTGATTGCTGCTAACGGTGTGGGTATGGTTATCAAAAACCCGATTGAAGGCTTGCC‑AGTGGCGCTCGGTGCGt                                                                       >  1:2668795/1‑93 (MQ=255)
                   gtatcggGCTGTTTCTGCTGCTGATTGCTGCTAACGGTGTGGGTATGGTTATCAAAAACCCGATTGAAGGCTTGCC‑AGTGGCGCTCGGTGCGt                                                                       >  1:3630580/1‑93 (MQ=255)
                    tatcggGCTGTTTCTGCTGCTGATTGTTGCTAACGGTGTGGGTATGGTTATCAAAATCCCGATTGAAGGCTTG‑CAAGTGGCGCTCGGTGCGtt                                                                      <  2:3211779/93‑1 (MQ=255)
                      tcggGCTGTTTCTGCTGCTGATTGCTGCTAACGGTGTGGGTATGGTTATCAAAAACCCGATTGAAGGCTTGCC‑AGTGGCGCTCGGTGCGTTTa                                                                    >  1:1918071/1‑93 (MQ=255)
                        gggCTGTTTCTGCTGCTGATTGCTGCTAACGGTGTGGGTATGGTTATCAAAACCCCGATTGAAGGCTTGCC‑AGTGGCGCTCGGTGCGTTTAcc                                                                  <  2:5970709/93‑1 (MQ=255)
                        gggCTGTTTCTGCTGCTGATTGCTGCTAACGGTGTGGGTATGGTTATCAAAAACCCGATTGAAGGCTTGCC‑AGTGGCGCTCGGTGCGTTTAcc                                                                  >  1:3543061/1‑93 (MQ=255)
                         ggCTGTTTCTGCTGCTGATTGCTGCTAACGGTGTGGGTATGGTTATCAAAAACCCGATTGAAGGCTTGCC‑AGTGGCGCTCGGTGCGTTTAcct                                                                 >  2:2998059/1‑93 (MQ=255)
                         ggCTGTTTCTGCTGCTGATTGCTGCTAACGGTGTGGGCATGGTTATCAAAAACCCGATTGAAGGCTTGCC‑AGTGGCGCTCGGTGCGTTTAcct                                                                 >  2:3995821/1‑93 (MQ=255)
                          gCTGTTTCTGCTGCTGATTGCTGCTAACGGTGTGGGTATGGTTATCAAAAACCCGATTGAAGGCTTGCC‑AGTGGCGCTCGGTGCGTTTAcctc                                                                <  1:1781566/93‑1 (MQ=255)
                           cTGTTTCTGCTGCTGATTGCTGCTAACGGTGTGGGTATGGTTATCAAAAACCCGATTGAAGGCTTGCC‑AGTGGCGCTCGGTGCGTTTAcctcc                                                               >  2:1379931/1‑93 (MQ=255)
                            tGTTTCTGCTGCTGATTGCTGCTAACGGTGTGGGTATGGTTATCAAAAACCCGATTGAAGGCTTGCC‑AGTGGCGCTTGGTGCGTTTAcctcct                                                              <  1:5162975/93‑1 (MQ=255)
                            tGTTTCTGCTGCTGATTGCTGCTAACGGTGTGGGTATGGTTATCAAAAACCCGATTGAAGGCTTGCC‑AGTGGCGCTCGGTGCGTTTAcctcct                                                              <  2:4835386/93‑1 (MQ=255)
                            tGTTTCTGCTGCTGATTGCTGCTAACGGTGTGGGTATGGTTATCAAAAACCCGATTGAAGGCTTGCC‑AGTGGCGCTCGGTGCGTTTAcctcct                                                              >  2:4771918/1‑93 (MQ=255)
                              tttCTGCTGCTGATTGCTGCTAACGGTGTGGGTATGGTTATCAAAAACCCGATTGAAGGCTTGCC‑AGTGGCGCTCGGTGCGTTTACCTCCTTc                                                            >  1:116974/1‑93 (MQ=255)
                               ttCTGCTGCTGATTGCTGCTAACGGTGTGGGTATGGTTATCAAAAACCCGATTGAAGGCTTGCC‑AGTGGCGCTCGGTGCGTTTACCTCCTTcc                                                           <  2:193417/93‑1 (MQ=255)
                                tCTGCTGCTGATTGCTGCTAACGGTGTGGGTATGGTTATCAAAAACCCGATTGAAGGCTTGCC‑AGTGGCGCTCGGTGCGTTTACCTCCTTccc                                                          >  1:5886938/1‑93 (MQ=255)
                                tCTGCTGCTGATTGCTGCTAACGGTGTGGGTATGGTTATCAAAAACCCGATTGAAGGCTTGCC‑AGTGGCGCTCGGTGCGTTTACCTCCTTccc                                                          >  2:5483191/1‑93 (MQ=255)
                                 ctgctgctgATTGCTGCTAACGGTGTGGGTATGGTTATCAAAAACCCGATTGAAGGCTTGCC‑AGTGGCGCTCGGTGCGTTTACCTCCTTCCCg                                                         >  1:3228120/1‑93 (MQ=255)
                                 ctgctgctgATTGCTGCTAACGGTGTGGGTATGGTTATCAAAAACCCGATTGAAGGCTTGCC‑AGTGGCGCTCGGTGCGTTTACCTCCTTCCCg                                                         <  2:2059666/93‑1 (MQ=255)
                                    ctgctgATTGCTGCTAACGGTGTGGGTATGGTTATCAAAAACCCGATTGAAGGCTTGCC‑AGTGGCGCTCGGTGCGTTTACCTCCTTCCCGGtg                                                      >  2:4625434/1‑93 (MQ=255)
                                     tgctgATTGCTGCTAACGGTGTGGGTATGGTTATCAAAAACCCGATTGAAGGCTTGCC‑AGTGGCGCTCGGTGCGTTTACCTCCTTCCCGGtga                                                     <  1:3400895/93‑1 (MQ=255)
                                      gctgATTGCTGCTAACGGTGTGGGTATGGTTATCAAAAACCCGATTGAAGGCTTGCC‑AGTGGCGCTCGGTGCGGTTACCTCCTGCCCGGggag                                                    >  2:3458866/1‑92 (MQ=255)
                                       ctgATTGCTGCTAACGGTGTGGGTATGGTTATCAAAAACCCGATTGAAGGCTTGCC‑AGTGGCGCTCGGTGCGTTTACCTCCTTCCCGGtgatg                                                   >  2:4660828/1‑93 (MQ=255)
                                        tgATTGCTGCTAACGGTGTGGGTATGGTTATCAAAAACCCGATTGAAGGCTTGCC‑AGTGGCGCTCGGTGCGTTTACCTCCTTCCCGGtgatga                                                  <  1:1309644/93‑1 (MQ=255)
                                         gATTGCTGCTAACGGTGTGGGTATGGTTATCAAAAACCCGATTGAAGGCTTGCC‑AGTGGCGCTCGGTGCGTTTACCTCCTTCCCGGtgatgat                                                 >  1:2780327/1‑93 (MQ=255)
                                         gATTGCTGCTAACGGTGTGGGTATGGTTATCAAAAACCCGATTGAAGGCTTGCC‑AGTGGCGCTCGGTGCGTTTACCTCCTTCCCGGtgatgat                                                 <  1:3487037/93‑1 (MQ=255)
                                          aTTGCTGCTAACGGTGTGGGTATGGTTATCAAAAACCCGATTGAAGGCTTGCC‑AGTGGCGCTCGGTGCGTTTACCTCCTTCCCGGtgatgatg                                                <  2:689002/93‑1 (MQ=255)
                                           ttGCTGCTAACGGTGTGGGTATGGTTATCAAAAACCCGATTGAAGGCTTGCC‑AGTGGCGCTCGGTGCGTTTACCTCCTTCCCGGtgatgatga                                               >  2:2261461/1‑93 (MQ=255)
                                            tgctgcTAACGGTGTGGGTATGGTTATCAAAAACCCGATTGAAGGCTTGCC‑AGTGGCGCTCGGTGCGTTTACCTCCTTCCCGGTGATGATGAg                                              <  2:3658867/93‑1 (MQ=255)
                                            tgctgcTAACGGTGTGGGTATGGTTATCAAAAACCCGATTGAAGGCTTGCC‑AGTGGCGCTCGGTGCGTTTACCTCCTTCCCGGTGATGATGAg                                              <  1:4379603/93‑1 (MQ=255)
                                             gctgctAACGGTGTGGGTATGGTTATCAAAAACCCGATTGAAGTCTTGCC‑AGTGGCGCTCGTTGCGTTTACCTCCTTCCCGGTGATGATGAGc                                             <  2:2063692/93‑1 (MQ=255)
                                             gctgctAACGGTGTGGGTATGGTTATCAAAAACCCGATTGAAGGCTTGCC‑AGTGGCGCTCGGTGCGTTTACCTCCTTCCCGGTGATGATGAGc                                             >  2:3590745/1‑93 (MQ=255)
                                              ctgctAACGGTGTGGGTATGGTTATCAAAAACCCGATTGAAGGCTTGCC‑AGTGGCGCTCGGTGCGTTTACCTCCTTCCCGGTGATGATGAGCt                                            <  2:3340169/93‑1 (MQ=255)
                                              ctgctAACGGTGTGGGTATGGTTATCAAAAACCCGATTGAAGGCTTGCC‑AGTGGCGCTCGGTGCGTTTACCTCCTTCCCGGTGATGATGAGCt                                            <  1:1108480/93‑1 (MQ=255)
                                               tgctAACGGTGTGGGTATGGTTATCAAAAACCCGATTGAAGGCTTGCC‑AGTGGCGCTCGGTGCGTTTACCTCCTTCCCGGTGATGATGAGCtt                                           >  1:6328232/1‑93 (MQ=255)
                                               tgctAACGGTGTGGGTATGGTTATCAAAAACCCGATTGAAGGCTTGCC‑AGTGGCGCTCGGTGCGTTTACCTCCTTCCCGGTGATGATGAGCtt                                           >  1:4649324/1‑93 (MQ=255)
                                                gctAACGGTGTGGGTATGGTTATCAAAAACCCGATTGAAGGCTTGCC‑AGTGGCGCTCGGTGCGTTTACCTCCTTCCCGGTGATGATGAGCTTg                                          >  1:2741446/1‑93 (MQ=255)
                                                 ctAACGGTGTGGGTATGGTTATCAAAAACCCGATTGAAGGCTTGCC‑AGTGGCGCTCGGTGCGTTTACCTCCTTCCCGGTGATGATGAGCTTGc                                         >  2:916172/1‑93 (MQ=255)
                                                  tAACGGTGTGGGTATGGTTATCAAAAACCCGATTGAAGGCTTGCC‑AGTGGCGCTCGGTGCGTTTACCTCCTTCCCGGTGATGATGAGCTTGCt                                        >  1:2369326/1‑93 (MQ=255)
                                                    aCGGTGTGGGTATGGTTATCAAAAACCCGATTGAAGGCTTGCC‑AGTGGCGCTCGGTGCGTTTACCTCCTTCCCGGTGATGATGAGCTTGCTgg                                      <  1:5381335/93‑1 (MQ=255)
                                                       gtgtGGGTATGGTTATCAAAAACCCGATTGAAGGCTTGCC‑AGTGGCGCTCGGTGCGTTTACCTCCTTCCCGGTGATGATGAGCTTGCTGGGGc                                   <  2:5164772/93‑1 (MQ=255)
                                                       gtgtGGGTATGGTTATCAAAAACCCGATTGAAGGCTTGCC‑AGTGGCGCTCGGTGCGTTTACCTCCTTCCCGGTGATGATGAGCTTGCTGGGGc                                   >  1:2631137/1‑93 (MQ=255)
                                                       gtgtGGGTATGGTTATCAAAAACCCGATTGAAGGCTTGCC‑AGTGGCGCTCGGTGCGTTTACCTCCTTCCCGGTGATGATGAGCTTGCTGGGGc                                   >  1:3882780/1‑93 (MQ=255)
                                                        tgtgGGTATGGTTATCAAAAACCCGATTGAAGGCGTGCC‑AGTGGCGCTCGGTGCGTTTACCTCCTTCCCGGTGATGATGAGCTTGCTGGGGCt                                  <  1:2043413/93‑1 (MQ=255)
                                                          tgGGTATGGTTATCAAAAACCCGATTGAAGGCTTGCC‑AGTGGCGCTCGGGGCGTTTACCTTCTTCCCGGTGATGATGAGCTTGCTGGGGCTgg                                >  2:2662423/1‑93 (MQ=255)
                                                            ggTATGGTTATCAAAAACCCGATTGAAGGCTTGCC‑AGTGGCGCTCGGTGCGTTTACCTCCTTCCCGGTGATGATGAGCTTGCTGGGGCTGGCg                              >  1:6009709/1‑93 (MQ=255)
                                                             gTATGGTTATCAAAAACCCGATTGAAGGCTTGCC‑AGTGGCGCTCGGTGCGTTTACCTCCTTCCCGGTGATGATGAGCTTGCTGGGGCTGGCgg                             <  2:6102035/93‑1 (MQ=255)
                                                             gTATGGTTATCAAAAACCCGATTGAAGGCTTGCC‑AGTGGCGCTCGGTGCGTTTACCTCCTTCCCGGTGATGATGAGCTTGCTGGGGCTGGCgg                             >  2:3734787/1‑93 (MQ=255)
                                                             gTATGGTTATCAAAAACCCGATTGAAGGCTTGCC‑AGTGGCGCTCGGTGCGTTTACCTCCTTCCCGGTGATGATGAGCTTGCTGGGGCTGGCgg                             >  2:4466445/1‑93 (MQ=255)
                                                             gTATGGTTATCAAAAACCCGATTGAAGGCTTGCC‑AGTGGCGCTCGGTGCGTTTACCTCCTTCCCGGTGATGATGAGCTTGCTGGGGCTGGCgg                             >  2:5123191/1‑93 (MQ=255)
                                                              tATGGTTATCAAAAACCCGATTGAAGGCTTGCC‑AGTGGCGCTCGGTGCGTTTACCTCCTTCCCGGTGATGATGAGCTTGCTGGGGCTGGCGGt                            <  2:751957/93‑1 (MQ=255)
                                                                tGGTTATCAAGAGCCCGATTGAAGGCTTGCC‑AGTGGCGCTCGGTGCGTTTACCTCCTTCCCGGTGATGATGAGCTTGCTGGGGCTGGCGGTCa                          <  1:862087/93‑1 (MQ=255)
                                                                 ggTTATCAAAAACCCGATTGAAGGCTTGCC‑AGTGGCGCTCGGTGCGTTTACCTCCTTCCCGGTGATGATGAGCTTGCTGGGGCTGGCGGTCAt                         >  2:1459295/1‑93 (MQ=255)
                                                                   ttATCAAAAACCCGATTGAAGGCTTGCC‑AGTGGCGCTCGGTGCGTTTACCTCCTTCCCGGTGATGATGAGCTTGCTGGGGCTGGCGGTCATCt                       <  2:6386688/93‑1 (MQ=255)
                                                                    tATCAAAAACCCGATTGAAGGCTTGCC‑AGTGGCGCTCGGTGCGTTTACCTCCTTCCCGGTGATGATGAGCTTGCTGGGGCTGGCGGTCATCtt                      >  1:2094141/1‑93 (MQ=255)
                                                                       cAAAAACCCGATTGAAGGCTTGCC‑AGTGGCGCTCGGTGCGTTTACCTCCTTCCCGGTGATGATGAGCTTGCTGGGGCTGGCGGTCATCTTCgg                   <  1:3773812/93‑1 (MQ=255)
                                                                       cAAAAACCCGATTGAAGGCTTGCC‑AGTGGCGCTCGGTGCGTTTACCTCCTTCCCGGTGATGATGAGCTTGCTGGGGCTGGCGGTCATCTTCgg                   >  2:4539891/1‑93 (MQ=255)
                                                                         aaaaCCCGATTGAAGGCTTGCC‑AGTGGCGCTCGGTGCGTTTACCTCCTTCCCGGTGATGATGAGCTTGCTGGGGCTGGCGGTCATCTTCGGcc                 <  1:1665133/93‑1 (MQ=255)
                                                                         aaaaCCCGATTGAAGGCTTGCC‑AGTGGCGCTCGGTGCGTTTACCTCCTTCCCGGTGATGATGAGCTTGCTGGGGCTGGCGGTCATCTTCGGcc                 >  1:3169033/1‑93 (MQ=255)
                                                                           aaCCCGATTGAAGGCTTGCC‑AGTGGCGCTCGGTGCGTTTACCTCCTTCCCGGTGATGATGAGCTTGCTGGGGCTGGCGGTCATCTTCGGCCTg               >  2:4985400/1‑93 (MQ=255)
                                                                           aaCCCGATTGAAGGCTTGCC‑AGTGGCGCTCGGTGCGTTTACCTCCTTCCCGGTGATGATGAGCTTGCTGGGGCTGGCGGTCATCTTCGGCCTg               >  1:1429126/1‑93 (MQ=255)
                                                                            aCCCGATTGAAGGCTTGCC‑AGTGGCGCTCGGTGCGTTTACCTCCTTCCCGGTGATGATGAGCTTGCTGGGGCTGGCGGTCATCTTCGGCCTgg              >  2:5906332/1‑93 (MQ=255)
                                                                            aCCCGATTGAAGGCTTGCC‑AGTGGCGCTCGGTGCGTTTACCTCCTTCCCGGTGATGATGAGCTTGCTGGGGCTGGCGGTCATCTTCGGCCTgg              >  1:6182524/1‑93 (MQ=255)
                                                                              ccGATTGAAGGCTTGCC‑AGTGGCGCTCGGTGCGTTTACCTCCTTCCCGGTGATGATGAGCTTGCTGGGGCTGGCGGTCATCTTCGGCCTGgag            >  2:3403427/1‑93 (MQ=255)
                                                                                gATTGAAGGCTTGCC‑AGTGGCGCTCGGTGCGTTTACCTCCTTCCCGGTGATGATGAGCTTGCTGGGGCTGGCGGTCATCTTCGGCCTGGAGaa          >  2:4390681/1‑93 (MQ=255)
                                                                                gATTGAAGGCTTGCC‑AGTGGCGCTCGGTGCGTTTACCTCCTTCCCGGTGATGATGAGCTTGCTGGGGCTGGCGGTCATCTTCGGCCTGGAGaa          >  2:3247904/1‑93 (MQ=255)
                                                                                  ttGAAGGCTTGCC‑AGTGGCGCTCGGTGCGTTTACCTCCTTCCCGGTGATGATGAGCTTGCTGGGGCTGGCGGTCATCTTCGGCCTGGAGAAgt        >  2:5593530/1‑93 (MQ=255)
                                                                                  ttGAAGGCTTGCC‑AGTGGCGCTCGGTGCGTTTACCTCCTTCCCGGTGATGATGAGCTTGCTGGGGCTGGCGGTCATCTTCGGCCTGGAGAAgt        >  2:4260086/1‑93 (MQ=255)
                                                                                    gAAGGCTTGCC‑AGTGGCGCTCGGTGCGTTTACCTCCTTCCCGGTGATGATGAGCTTGCTGGGGCTGGCGGTCATCTTCGGCCTGGAGAAgtgt      >  1:1496953/1‑93 (MQ=255)
                                                                                    gAAGGCTTGCC‑AGTGGCGCTCGGTGCGTTTACCTCCTTCCCGGTGATGATGAGCTTGCTGGGGCTGGCGGTCATCTTCGGCCTGGAGAAgtgt      >  1:4941211/1‑93 (MQ=255)
                                                                                    gAAGGCTTGCC‑AGTGGCGCTCGGTGCGTTTACCTCCTTCCCGGTGATGATGAGCTTGCTGGGGCTGGAGGTCATCTTCGGCCTGGAGAAgtgt      >  2:1777376/1‑93 (MQ=255)
                                                                                     aaGGCTTGCC‑AGTGGCGCTCGGTGCGTTTACCTCCTTCCCGGTGATGATGAGCTTGCTGGGGCTGGCGGTCATCTTCGGCCTGGAGAAGTGTc     >  1:4142647/1‑93 (MQ=255)
                                                                                      aGGCTTGCC‑AGTGGCGCTCGGTGCGTTTACCTCCTTCCCGGTGATGATGAGCTTGCTGGGGCTGGCGGTCATCTTCGGCCTGGAGAAGTGTcg    >  2:2440457/1‑93 (MQ=255)
                                                                                       ggCTTGCC‑AGTGGCGCTCGGTGCGTTTACCTCCTTCCCGGTGATGATGAGCTTGCTGGGGCTGGCGGTCATCTTCGGCCTGGAGAAGTGTcgc   <  1:4506921/93‑1 (MQ=255)
                                                                                       ggCTTGCC‑AGTGGCGCTCGGTGCGTTTACCTCCTTCCCGGTGATGATGAGCTTGCTGGGGCTGGCGGTCATCTTCGGCCTGGAGAAGTGTcgc   >  1:6116839/1‑93 (MQ=255)
                                                                                        gCTTGCC‑AGTGGCGCTCGGTGCGTTTACCTCCTTCCCGGTGATGATGAGCTTGCTGGGGCTGGCGGTCATCTTCGGCCTGGAGAAGTGTcgcg  >  2:5860599/1‑93 (MQ=255)
                                                                                        gCTTGCC‑AGTGGCGCTCGGTGCGTTTACCTCCTTCCCGGTGATGATGAGCTTGCTGGGGCTGGCGGTCATCTTCGGCCTGGAGAAGTGTcgcg  <  2:4850919/93‑1 (MQ=255)
                                                                                             |                                                                                        
GCTCACGGTACAGGTATCGGTATCGGGCTGTTTCTGCTGCTGATTGCTGCTAACGGTGTGGGTATGGTTATCAAAAACCCGATTGAAGGCTTG‑C‑AGTGGCGCTCGGTGCGTTTACCTCCTTCCCGGTGATGATGAGCTTGCTGGGGCTGGCGGTCATCTTCGGCCTGGAGAAGTGTCGCG  >  NC_000913/3025583‑3025762

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
GCTCACGGTACAGGTATCGGTATCGGGCTGTTTCTGCTGCTGATTGCTGCTAACGGTGTGGGTATGGTTATCAAAAACCCGATTGAAGGCTTG‑CAGTGGCGCTCGGTGCGTTTACCTCCTTCCCGGTGATGATGAGCTTGCTGGGGCTGGCGGTCATCTTCGGCCTGGAGAAGTGTCGCGTACCCG  >  NC_000913/3025583‑3025768
                                                                                              |                                                                                            
GCTCACGGTACAGGTATCGGTATCGGGCTGTTTCTGCTGCTGATTGCTGCTAACGGTGTGGGTATGGTTATCAAAAACCCGATTGAAGGCTTG‑C                                                                                              >  SRR4302537.5116121/1‑94 (MQ=60)
GCTCACGGTACAGGTATCGGTATCGGGCTGTTTCTGCTGCTGATTGCTGCTAACGGTGTGGGTATGGTTATCAAAAACCCGATTGAAGGCTTG‑C                                                                                              >  SRR4302537.372234/1‑94 (MQ=60)
   CACGCTACAGGTATCGGTATCGGGCTGTTTCTGCTGCTGATTGCTGCTAACGGTGTGGGTATGGTTATCAAAAACCCGATTGAAGGCTTG‑Ccag                                                                                           <  SRR4302537.2045136/94‑4 (MQ=60)
    ACGGTACAGGTATCGGTATCGGGCTGTTTCTGCTGCTGATTGCTGCTAACGGTGTGGGTATGGTTATCAAAAACCCGATTGAAGGCTTGCCAGT                                                                                           <  SRR4302537.4336540/94‑1 (MQ=60)
      GGTACAGGTATCGGTATCGGGCTGTTTCTGCTGCTGATTGCTGCTAACGGTGTGGGTATGGTTATCAAAAACCCGATTGAAGGCTTGCCAGTGG                                                                                         >  SRR4302537.1523001/1‑94 (MQ=60)
       GTACAGGTATCGGTATCGGGCTGTTTCTGCTGCTGATTGCTGCTAACGGTGTGGGTATGGTTATCAAAAACCCGATTGAAGGCTTGCCAGTGGC                                                                                        <  SRR4302537.6092349/94‑1 (MQ=60)
       GTACAGGTATCGGTATCGGGCTGTTTCTGCTGCTGATTGCTGCTAACGGTGTGGGTATGGTTATCAAAAACCCGATTGAAGGCTTGCCAGTGGC                                                                                        >  SRR4302537.2339437/1‑94 (MQ=60)
        TACAGGTATCGGTATCGGGCTGTTTCTGCTGCTGATTGCTGCTAACGGTGTGGGTATGGTTATCAAAAACCCGATTGAAGGCTTGCCAGTGGCG                                                                                       >  SRR4302537.1268265/1‑94 (MQ=60)
          CAGGTATCGGTATCGGGCTGTTTCTGCTGCTGATTGCTGCTAACGGTGTGGGTATGGTTATCAAAAACCCGATTGAAGGCTTGCCAGTGGCGCT                                                                                     <  SRR4302537.61807/94‑1 (MQ=60)
          CAGGTATCGGTATCGGGCTGTTTCTGCTGCTGATTGCTGCTAACGGTGTGGGTATGGTTATCAAAAACCCGATTGAAGGCTTGCCAGTGGCGCT                                                                                     >  SRR4302537.265820/1‑94 (MQ=60)
          CAGGTATCGGTATCGGGCTGTTTCTGCTGCTGATTGCTGCTAACGGTGTGGGTATGGTTATCAAAAACCCGATTGAAGGCTTGCCAGTGGCGCT                                                                                     >  SRR4302537.1678808/1‑94 (MQ=60)
           AGGTATCGGTATCGGGCTGTTTCTGCTGCTGATTGCTGCTAACGGTGTGGGTATGGTTATCAAAAACCCGATTGAAGGCTTGCCAGTGGCGCTC                                                                                    <  SRR4302537.5690882/94‑1 (MQ=60)
            GGTATCGGTATCGGGCTGTTTCTGCTGCTGATTGCTGCTAACGGTGTGGGTATGGTTATCAAAAACCCGATTGAAGGCTTGCCAGTGGCGCTCG                                                                                   >  SRR4302537.96173/1‑94 (MQ=60)
               ATCGGTATCGGGCTGTTTCTGCTGCTGATTGCTGCTAACGGTGTGGGTATGGTTATCAAAAACCCGATTGAAGGCTTGCCAGTGGCGCTCGGTG                                                                                >  SRR4302537.3556655/1‑94 (MQ=60)
               ATCGGTATCGGGCTGTTTCTGCTGCTGATTGCTGCTAACGGTGTGGGTATGGTTATCAAAAACCCGATTGAAGGCTTGCCAGTGGCGCTCGGTG                                                                                >  SRR4302537.2711065/1‑94 (MQ=60)
               ATCGGTATCGGGCTGTTTCTGCTGCTGATTGCTGCTAACGGTGTGGGTATGGTTATCAAAAACCCGATTGAAGGCTTGCCAGTGGCGCTCGGTG                                                                                >  SRR4302537.1282669/1‑94 (MQ=60)
                TCGGTATCGGGCTGTTTCTGCTGCTGATTGCTGCTAACGGTGTGGGTATGGTTATCAAAAACCCGATTGAAGGCTTGCCAGTGGCGCTCGGTGC                                                                               <  SRR4302537.3076072/94‑1 (MQ=60)
                TCGGTATCGGGCTGTTTCTGCTGCTGATTGCTGCTAACGGTGTGGGTATGGTTATCAAAAACCCGATTGAAGGCTTGCCAGTGGCGCTCGGTGC                                                                               >  SRR4302537.3596532/1‑94 (MQ=60)
                 CGGTATCGGGCTGTTTCTGCTGCTGATTGCTGCTAACGGTGTGGGTATGGTTATCAAAAACCCGATTGAAGGCTTGCCAGTGGCGCTCGGTGCG                                                                              <  SRR4302537.2854226/94‑1 (MQ=60)
                  GGTATCGGGCTGTTTCTGCTGCTGATTGCTGCTAACGGTGTGGGTATGGTTATCAAAAACCCGATTGAAGGCTTGCCAGTGGCGCTCGGTGCGT                                                                             >  SRR4302537.5653703/1‑94 (MQ=60)
                  GGTATCGGGCTGTTTCTGCTGCTGATTGCTGCTAACGGTGTGGGTATGGTTATCAAAAACCCGATTGAAGGCTTGCCAGTGGCGCTCGGTGCGT                                                                             >  SRR4302537.2687439/1‑94 (MQ=60)
                  GGTATCGGGCTGTTTCTGCTGCTGATTGCTGCTAACGGTGTGGGTATGGTTATCAAAAACCCGATTGAAGGCTTGCCAGTGGCGCTCGGTGCGT                                                                             >  SRR4302537.5134448/1‑94 (MQ=60)
                  GGTATCGGGCTGTTTCTGCTGCTGATTGCTGCTAACGGTGTGGGTATGGTTATCAAAAACCCGATTGAAGGCTTGCCAGTGGCGCTCGGTGCGT                                                                             >  SRR4302537.3656163/1‑94 (MQ=60)
                  GGTATCGGGCTGTTTCTGCTGCTGATTGCTGCTAACGGTGTGGGTATGGTTATCAAAAACCCGATTGAAGGCTTGCCAGTGGCGCTCGGTGCGT                                                                             >  SRR4302537.1554609/1‑94 (MQ=60)
                  GGTATCGGGCTGGTTCTGCTGCTGATTGCTGCTAACGGTGTGGGTATGGTTATCAAAAACCCGATTGAAGGCTTGCCAGTGGCGCTCGGTGCGT                                                                             <  SRR4302537.4897556/94‑1 (MQ=60)
                   GTATCGGGCTGTTTCTGCTGCTGATTGCTGCTAACGGTGTGGGTATGGTTATCAAAAACCCGATTGAAGGCTTGCCAGTGGCGCTCGGTGCGTT                                                                            >  SRR4302537.277146/1‑94 (MQ=60)
                    TATCTGGCTGTTTCTGCTGCTGATTGCTGCTAACGGTGTGGGTATGGTTATCAAAAACCCGATTGAAGGCTTGCCAGTGGCGCTCGGTGCGTTT                                                                           <  SRR4302537.4271963/94‑1 (MQ=60)
                     ATCGGGCTGTTTCTGCTGCTGATTGCTGCTAACGGTGTGGGTATGGTTATCAAAAACCCGATTGAAGGCTTGCCAGTGGCGCTCGGTGCGTTTA                                                                          >  SRR4302537.1931388/1‑94 (MQ=60)
                       CGGGCTGTTTCTGCTGCTGATTGCTGCTAACGGTGTGGGTATGGTTATCAAAAACCCGATTGAAGGCTTGCCAGTGGCGCTCGGTGCGTTTACC                                                                        >  SRR4302537.3568090/1‑94 (MQ=60)
                        GGGCTGTTTCTGCTGCTGATTGCTGCTAACGGTGTGGGTATGGTTATCAAAAACCCGATTGAAGGCTTGCCAGTGGCGCTCGGTGCGTTTACCT                                                                       >  SRR4302537.3640122/1‑94 (MQ=60)
                          GCTGTTTCTGCTGCTGATTGCTGCTAACGGTGTGGGTATGGTTATCAAAAACCCGATTGAAGGCTTGCCAGTGGCGCTCGGTGCGTTTACCTCC                                                                     >  SRR4302537.2841948/1‑94 (MQ=60)
                          GCTGTTTCTGCTGCTGATTGCTGCTAACGGTGTGGGTATGGTTATCAAAAACCCGATTGAAGGCTTGCCAGTGGCGCTCGGTGCGTTTACCTCC                                                                     <  SRR4302537.1793982/94‑1 (MQ=60)
                           CTGTTTCTGCTGCTGATTGCTGCTAACGGTGTGGGTATGGTTATCAAAAACCCGATTGAAGGCTTGCCAGTGGCGCTCGGTGCGTTTACCTCCT                                                                    >  SRR4302537.2213262/1‑94 (MQ=60)
                           CTGTTTCTGCTGCTGATTGCTGCTAACGGTGTGGGTATGGTTATCAAAAACCCGATTGAAGGCTTGCCAGTGGCGCTCGGTGCGTTTACCTCCT                                                                    >  SRR4302537.2054802/1‑94 (MQ=60)
                           CTGTTTCTGCTGCTGATTGCTGCTAACGGTGTGGGTATGGTTATCAAAAACCCGATTGAAGGCTTGCCAGTGGCGCTCGGTGCGTTTACCTCCT                                                                    >  SRR4302537.2884647/1‑94 (MQ=60)
                            TGTTTCTGCTGCTGATTGCTGCTAACGGTGTGGGTATGGTTATCAAAAACCCGATTGAAGGCTTGCCAGTGGCGCTTGGTGCGTTTACCTCCTT                                                                   <  SRR4302537.5198559/94‑1 (MQ=60)
                            TGTTTCTGCTGCTGATTGCTGCTAACGGTGTGGGTATGGTTATCAAAAACCCGATTGAAGGCTTGCCAGTGGCGCTCGGTGCGTTTACCTCCTT                                                                   >  SRR4302537.5827814/1‑94 (MQ=60)
                             GTTTCTGCTGCTGATTGCTGCTAACGGTGTGGGTATGGTTATCAAAAACCCGATTGAAGGCTTGCCAGTGGCGCTCGGTGCGTTTACCTCCTTC                                                                  >  SRR4302537.117819/1‑94 (MQ=60)
                               TTCTGCTGCTGATTGCTGCTAACGGTGTGGGTATGGTTATCAAAAACCCGATTGAAGGCTTGCCAGTGGCGCTCGGTGCGTTTACCTCCTTCCC                                                                >  SRR4302537.5927330/1‑94 (MQ=60)
                                TCTGCTGCTGATTGCTGCTAACGGTGTGGGTATGGTTATCAAAAACCCGATTGAAGGCTTGCCAGTGGCGCTCGGTGCGTTTACCTCCTTCCCG                                                               >  SRR4302537.3250868/1‑94 (MQ=60)
                                   GCTGCTGATTGCTGCTAACGGTGTGGGTATGGTTATCAAAAACCCGATTGAAGGCTTGCCAGTGGCGCTCGGTGCGTTTACCTCCTTCCCGGTG                                                            >  SRR4302537.2021545/1‑94 (MQ=60)
                                     TGCTGATTGCTGCTAACGGTGTGGGTATGGTTATCAAAAACCCGATTGAAGGCTTGCCAGTGGCGCTCGGTGCGTTTACCTCCTTCCCGGTGAT                                                          <  SRR4302537.3424983/94‑1 (MQ=60)
                                        TGATTGCTGCTAACGGTGTGGGTATGGTTATCAAAAACCCGATTGAAGGCTTGCCAGTGGCGCTCGGTGCGTTTACCTCCTTCCCGGTGATGAT                                                       <  SRR4302537.1318761/94‑1 (MQ=60)
                                        TGATTGCTGCTAACGGTGTGGGTATGGTTATCAAAAACCCGATTGAAGGCTTGCCAGTGGCGCTCGGTGCGTTTACCTCCTTCCCGGTGATGAT                                                       >  SRR4302537.2799770/1‑94 (MQ=60)
                                         GATTGCTGCTAACGGTGTGGGTATGGTTATCAAAAACCCGATTGAAGGCTTGCCAGTGGCGCTCGGTGCGTTTACCTCCTTCCCGGTGATGATG                                                      <  SRR4302537.3511695/94‑1 (MQ=60)
                                          ATTGCTGCTAACGGTGTGGGTATGGTTATCAAAAACCCGATTGAAGGCTTGCCAGTGGCGCTCGGTGCGTTTACCTCCTTCCCGGTGATGATGA                                                     >  SRR4302537.4617700/1‑94 (MQ=60)
                                            TGCTGCTAACGGTGTGGGTATGGTTATCAAAAACCCGATTGAAGGCTTGCCAGTGGCGCTCGGTGCGTTTACCTCCTTCCCGGTGATGATGAGC                                                   <  SRR4302537.4409832/94‑1 (MQ=60)
                                            TGCTGCTAACGGTGTGGGTATGGTTATCAAAAACCCGATTGAAGGCTTGCCAGTGGCGCTCGGTGCGTTTACCTCCTTCCCGGTGATGATGAGC                                                   >  SRR4302537.269258/1‑94 (MQ=60)
                                              CTGCTAACGGTGTGGGTATGGTTATCAAAAACCCGATTGAAGGCTTGCCAGTGGCGCTCGGTGCGTTTACCTCCTTCCCGGTGATGATGAGCTT                                                 >  SRR4302537.4681466/1‑94 (MQ=60)
                                              CTGCTAACGGTGTGGGTATGGTTATCAAAAACCCGATTGAAGGCTTGCCAGTGGCGCTCGGTGCGTTTACCTCCTTCCCGGTGATGATGAGCTT                                                 >  SRR4302537.6371511/1‑94 (MQ=60)
                                              CTGCTAACGGTGTGGGTATGGTTATCAAAAACCCGATTGAAGGCTTGCCAGTGGCGCTCGGTGCGTTTACCTCCTTCCCGGTGATGATGAGCTT                                                 >  SRR4302537.4445748/1‑94 (MQ=60)
                                              CTGCTAACGGTGTGGGTATGGTTATCAAAAACCCGATTGAAGGCTTGCCAGTGGCGCTCGGTGCGTTTACCTCCTTCCCGGTGATGATGAGCTT                                                 <  SRR4302537.1116142/94‑1 (MQ=60)
                                               TGCTAACGGTGTGGGTATGGTTATCAAAAACCCGATTGAAGGCTTGCCAGTGGCGCTCGGTGCGTTTACCTCCTTCCCGGTGATGATGAGCTTG                                                >  SRR4302537.2760572/1‑94 (MQ=60)
                                                 CTAACGGTGTGGGTATGGTTATCAAAAACCCGATTGAAGGCTTGCCAGTGGCGCTCGGTGCGTTTACCTCCTTCCCGGTGATGATGAGCTTGCT                                              <  SRR4302537.2406038/94‑1 (MQ=60)
                                                 CTAACGGTGTGGGTATGGTTATCAAAAACCCGATTGAAGGCTTGCCAGTGGCGCTCGGTGCGTTTACCTCCTTCCCGGTGATGATGAGCTTGCT                                              >  SRR4302537.2386025/1‑94 (MQ=60)
                                                  TAACGTTGTGGGTATGGTTATCAAAAACCCGATTGAAGGCTTGCCAGTGGCGCTCGGTGCGTTTACCTCCTTCCCGGTGATGATGAGCTTGCTG                                             <  SRR4302537.3249943/94‑1 (MQ=60)
                                                    ACGGTGTGGGTATGGTTATCAAAAACCCGATTGAAGGCTTGCCAGTGGCGCTCGGTGCGTTTACCTCCTTCCCGGTGATGATGAGCTTGCTGGG                                           <  SRR4302537.5418134/94‑1 (MQ=60)
                                                    ACGGTGTGGGTATGGTTATCAAAAACCCGATTGAAGGCTTGCCAGTGGCGCTCGGTGCGTTTACCTCCTTCCCGGTGATGATGAGCTTGCTGGG                                           <  SRR4302537.4908232/94‑1 (MQ=60)
                                                    ACGGTGTGGGTATGGTTATCAAAAACCCGATTGAAGGCTTGCCAGTGGCGCTCGGTGCGTTTACCTCCTTCCCGGTGATGATGAGCTTGCTGGG                                           <  SRR4302537.5964664/94‑1 (MQ=60)
                                                     CGGTGTGGGTATGGTTATCAAAAACCCGATTGAAGGCTTGCCAGTGGCGCTCGGTGCGTTTACCTCCTTCCCGGTGATGATGAGCTTGCTGGGG                                          <  SRR4302537.1264752/94‑1 (MQ=60)
                                                     CGGTGTGGGTATGGTTATCAAAAACCCGATTGAAGGCTTGCCAGTGGCGCTCGGTGCGTTTACCTCCTTCCCGGTGATGATGAGCTTGCTGGGG                                          >  SRR4302537.3590820/1‑94 (MQ=60)
                                                      GGTGTGGGTATGGTTATCAAAAACCCGATTGAAGGCTTGCCAGTGGCGCTCGGTGCGTTTACCTCCTTCCCGGTGATGATGAGCTTGCTGGGGC                                         >  SRR4302537.3909943/1‑94 (MQ=60)
                                                      GGTGTGGGTATGGTTATCAAAAACCCGATTGAAGGCTTGCCAGTGGCGCTCGGTGCGTTTACCTCCTTCCCGGTGATGATGAGCTTGCTGGGGC                                         >  SRR4302537.2649559/1‑94 (MQ=60)
                                                       GTGTGGGTATGGTTATCAAAAACCCGATTGAAGGCTTGCCAGTGGCGCTCGGTGCGTTTACCTCCTTCCCGGTGATGATGAGCTTGCTGGGGCT                                        <  SRR4302537.3999242/94‑1 (MQ=60)
                                                        TGTGGGTATGGTTATCAAAAACCCGATTGAAGGCGTGCCAGTGGCGCTCGGTGCGTTTACCTCCTTCCCGGTGATGATGAGCTTGCTGGGGCTG                                       <  SRR4302537.2057712/94‑1 (MQ=60)
                                                          TGGGTATGGTTATCAAAAACCCGATTGAAGGCTTGCCAGTGGCGCTCGGTGCGTTTACCTCCTTCCCGGTGATGATGAGCTTGCTGGGGCTGGC                                     >  SRR4302537.2777348/1‑94 (MQ=60)
                                                           GGGTATGGTTATCAAAAACCCGATTGAAGGCTTGCCAGTGGCGCTCGGTGCGTTTACCTCCTTCCCGGTGATGATGAGCTTGCTGGGGCTGGCG                                    >  SRR4302537.6050999/1‑94 (MQ=60)
                                                            GGTATGGTTATCAAAAACCCGATTGAAGGCTTGCCAGTGGCGCTCGGTGCGTTTACCTCCTTCCCGGTGATGATGAGCTTGCTGGGGCTGGCGG                                   <  SRR4302537.3350534/94‑1 (MQ=60)
                                                               ATGGTTATCAAAAACCCGATTGAAGGCTTGCCAGTGGCGCTCGGTGCGTTTACCTCCTTCCCGGTGATGATGAGCTTGCTGGGGCTGGCGGTCA                                >  SRR4302537.4378341/1‑94 (MQ=60)
                                                               ATGGTTATCAAAAACCCGATTGAAGGCTTGCCAGTGGCGCTCGGTGCGTTTACCTCCTTCCCGGTGATGATGAGCTTGCTGGGGCTGGCGGTCA                                >  SRR4302537.5007405/1‑94 (MQ=60)
                                                                TGGTTATCAAGAGCCCGATTGAAGGCTTGCCAGTGGCGCTCGGTGCGTTTACCTCCTTCCCGGTGATGATGAGCTTGCTGGGGCTGGCGGTCAT                               <  SRR4302537.867998/94‑1 (MQ=60)
                                                                TGGTTATCAAAAACCCGATTGAAGGCTTGCCAGTGGCGCTCGGTGCGTTTACCTCCTTCCCGGTGATGATGAGCTTGCTGGGGCTGGCGGTCAT                               <  SRR4302537.4712360/94‑1 (MQ=60)
                                                                  GTTATCAAAAACCCGATTGAAGGCTTGCCAGTGGCGCTCGGTGCGTTTACCTCCTTCCCGGTGATGATGAGCTTGCTGGGGCTGGCGGTCATCT                             <  SRR4302537.6238742/94‑1 (MQ=60)
                                                                   TTATCAAAAACCCGATTGAAGGCTTGCCAGTGGCGCTCGGTGCGTTTACCTCCTTCCCGGTGATGATGAGCTTGCTGGGGCTGGCGGTCATCTT                            >  SRR4302537.2108910/1‑94 (MQ=60)
                                                                    TATCAAAAACCCGATTGAAGGCTTGCCAGTGGCGCTCGGTGCGTTTACCTCCTTCCCGGTGATGATGAGCTTGCTGGGGCTGGCGGTCATCTTC                           <  SRR4302537.3872675/94‑1 (MQ=60)
                                                                     ATCAAAAACCCGATTGAAGGCTTGCCAGTGGCGCTCGGTGCGTTTACCTCCTTCCCGGTGATGATGAGCTTGCTGGGGCTGGCGGTCATCTTCG                          >  SRR4302537.885024/1‑94 (MQ=60)
                                                                      TCAAAAACCCGATTGAAGGCTTGCCAGTGGCGCTCGGTGCGTTTACCTCCTTCCCGGTGATGATGAGCTTGCTGGGGCTGGCGGTCATCTTCGG                         >  SRR4302537.2726348/1‑94 (MQ=60)
                                                                      TCAAAAACCCGATTGAAGGCTTGCCAGTGGCGCTCGGTGCGTTTACCTCCTTCCCGGTGATGATGAGCTTGCTGGGGCTGGCGGTCATCTTCGG                         >  SRR4302537.314586/1‑94 (MQ=60)
                                                                       CAAAAACCCGATTGAAGGCTTGCCAGTGGCGCTCGGTGCGTTTACCTCCTTCCCGGTGATGATGAGCTTGCTGGGGCTGGCGGTCATCTTCGGC                        <  SRR4302537.3800305/94‑1 (MQ=60)
                                                                        AAAAACCCGATTGAAGGCTTGCCAGTGGCGCTCGGTGCGTTTACCTCCTTCCCGGTGATGATGAGCTTGCTGGGGCTGGCGGTCATCTTCGGCC                       >  SRR4302537.3869566/1‑94 (MQ=60)
                                                                        AAAAACCCGATTGAAGGCTTGCCAGTGGCGCTCGGTGCGTTTACCTCCTTCCCGGTGATGATGAGCTTGCTGGGGCTGGCGGTCATCTTCGGCC                       >  SRR4302537.3191299/1‑94 (MQ=60)
                                                                         AAAACCCGATTGAAGGCTTGCCAGTGGCGCTCGGTGCGTTTACCTCCTTCCCGGTGATGATGAGCTTGCTGGGGCTGGCGGTCATCTTCGGCCT                      <  SRR4302537.1676801/94‑1 (MQ=60)
                                                                          AAACCCGATTGAAGGCTTGCCAGTGGCGCTCGGTGCGTTTACCTCCTTCCCGGTGATGATGAGCTTGCTGGGGCTGGCGGTCATCTTCGGCCTG                     >  SRR4302537.1439075/1‑94 (MQ=60)
                                                                          AAACCCGATTGAAGGCTTGCCAGTGGCGCTCGGTGCGTTTACCTCCTTCCCGGTGATGATGAGCTTGCTGGGGCTGGCGGTCATCTTCGGCCTG                     >  SRR4302537.5229214/1‑94 (MQ=60)
                                                                           AACCCGATTGAAGGCTTGCCAGTGGCGCTCGGTGCGTTTACCTCCTTCCCGGTGATGATGAGCTTGCTGGGGCTGGCGGTCATCTTCGGCCTGG                    >  SRR4302537.6225110/1‑94 (MQ=60)
                                                                           AACCCGATTGAAGGCTTGCCAGTGGCGCTCGGTGCGTTTACCTCCTTCCCGGTGATGATGAGCTTGCTGGGGCTGGCGGTCATCTTCGGCCTGG                    >  SRR4302537.639447/1‑94 (MQ=60)
                                                                            ACCCGATTGAAGGCTTGCCAGTGGCGCTCGGTGCGTTTACCTCCTTCCCGGTGATGATGAGCTTGCTGGGGCTGGCGGTCATCTTCGGCCTGGA                   <  SRR4302537.689234/94‑1 (MQ=60)
                                                                            ACCCGATTGAAGGCTTGCCAGTGGCGCTCGGTGCGTTTACCTCCTTCCCGGTGATGATGAGCTTGCTGGGGCTGGCGGTCATCTTCGCCCTGGA                   >  SRR4302537.112789/1‑94 (MQ=60)
                                                                              CCGATTGAAGGCTTGCCAGTGGCGCTCGGTGCGTTTAACTCCTTCCCGGTGATGATGAGCTTGCTGGGGCTGGCGGTCATCTTCGGTCTGGAGA                 >  SRR4302537.5892783/1‑94 (MQ=60)
                                                                                GATTGAAGGCTTGCCAGTGGCGCTCGGTGCGTTTACCTCCTTCCCGGTGATGATGAGCTTGCTGGGGCTGGCGGTCATCTTCGGCCTGGAGAAG               <  SRR4302537.562763/94‑1 (MQ=60)
                                                                                GATTGAAGGCTTGCCAGTGGCGCTCGGTGCGTTTACCTCCTTCCCGGTGATGATGAGCTTGCTGGGGCTGGCGGTCATCTTCGGCCTGGAGAAG               >  SRR4302537.4837332/1‑94 (MQ=60)
                                                                                GATTGAAGGCTTGCCAGTGGCGCTCGGTGCGTTTACCTCCTTCCCGGTGATGATGAGCTTGCTGGGGCTGGCGGTCATCTTCGGCCTGGAGAAG               >  SRR4302537.3438544/1‑94 (MQ=60)
                                                                                 ATTGAAGGCTTGCCAGTGGCGCTCGGTGCGTTTACCTCCTTCCCGGTGATGATGAGCTTGCTGGGGCTGGCGGTCATCTTCGGCCTGGAGAAGT              >  SRR4302537.1454613/1‑94 (MQ=60)
                                                                                  TTGAAGGCTTGCCAGTGGCGCTCGGTGCGTTTACCTCCTTCCCGGTGATGATGAGCTTGCTGGGGCTGGCGGTCATCTTCGGCCTGGAGAAGTG             <  SRR4302537.2103662/94‑1 (MQ=60)
                                                                                  TTGAAGGCTTGCCAGTGGCGCTCGGTGCGTTTACCTCCTTCCCGGTGATGATGAGCTTGCTGGGGCTGGCGGTCATCTTCGGCCTGGAGAAGTG             >  SRR4302537.3453219/1‑94 (MQ=60)
                                                                                   TGAAGGCTTGCCAGTGGCGCTCGGTGCGTTTACCTCCTTCCCGGTGATGATGAGCTTGCTGGGGCTGGCGGTCATCTTCGGCCTGGAGAAGTGT            >  SRR4302537.4975286/1‑94 (MQ=60)
                                                                                   TGAAGGCTTGCCAGTGGCGCTCGGTGCGTTTACCTCCTTCCCGGTGATGATGAGCTTGCTGGGGCTGGCGGTCATCTTCGGCCTGGAGAAGTGT            >  SRR4302537.1507395/1‑94 (MQ=60)
                                                                                    GAAGGCTTGCCAGTGGCGCTCGGTGCGTTTACCTCCTTCCCGGTGATGATGAGCTTGCTGGGGCTGGCGGTCATCTTCGGCCTGGAGAAGTGTC           >  SRR4302537.4171545/1‑94 (MQ=60)
                                                                                     AAGGCTTGCCAGTGGCGCTCGGTGCGTTTACCTCCTTCCCGGTGATGATGAGCTTGCTGGGGCTGGCGGTCATCTTCGGCCTGGAGAAGTGTCG          >  SRR4302537.1890609/1‑94 (MQ=60)
                                                                                      AGGCTTGCCAGTGGCGCTCGGTGCGTTTACCTCCTTCCCGGTGATGATGAGCTTGCTGGGGCTGGCGGTCATCTTCGGCCTGGAGAAGTGTCGC         >  SRR4302537.6158876/1‑94 (MQ=60)
                                                                                       GGCTTGCCAGTGGCGCTCGGTGCGTTTACCTCCTTCCCGGTGATGATGAGCTTGCTGGGGCTGGCGGTCATCTTCGGCCTGGAGAAGTGTCGCG        <  SRR4302537.4538087/94‑1 (MQ=60)
                                                                                        GCTTGCCAGTGGCGCTCGGTGCGTTTACCTCCTTCCCGGTGATGATGAGCTTGCTGGGGCTGGCGGTCATCTTCGGCCTGGAGAAGTGTCGCGT       >  SRR4302537.2876924/1‑94 (MQ=60)
                                                                                          TTGCCAGTGGCGCTCGGTGCGTTTACCTCCTTCCCGGTGATGATGAGCTTGCTGGGGCTGGCGGTCATCTTCGGCCTGGAGAAGTGTCGCGTAC     <  SRR4302537.1480277/94‑1 (MQ=60)
                                                                                           tgcCAGTGGCGCTCGGTGCGTTTACCTCCTTCCCGGTGATGATGAGCTTGCTGGGGCTGGCGGTCATCTTCGGCCTGGAGAAGTGTCGCGTACC    >  SRR4302537.3450591/4‑94 (MQ=60)
                                                                                            gcCAGTGGCGCTCGGTGCGTTTACCTCCTTCCCGGTGATGATGAGCTTGCTGGGGCTGGCGGTCATCTTCGGCCTGGAGAAGTGTCGCGTACCC   <  SRR4302537.5862981/92‑1 (MQ=60)
                                                                                            C‑CAGTGTCGCTCGGTGCCTTTACCTCCTTCCCGGTGATGATGAGCTTGCTGGGGCTGGAGGTCATCTTCGGCCTGGAGAAGTGTCGCGTACCCG  <  SRR4302537.4326611/94‑1 (MQ=60)
                                                                                              |                                                                                            
GCTCACGGTACAGGTATCGGTATCGGGCTGTTTCTGCTGCTGATTGCTGCTAACGGTGTGGGTATGGTTATCAAAAACCCGATTGAAGGCTTG‑CAGTGGCGCTCGGTGCGTTTACCTCCTTCCCGGTGATGATGAGCTTGCTGGGGCTGGCGGTCATCTTCGGCCTGGAGAAGTGTCGCGTACCCG  >  NC_000913/3025583‑3025768

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑