Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I2 R1 882 254.4 12957588 98.0% 12698436 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,059,022 T→C G51G (GGT→GGC)  sbm → methylmalonyl‑CoA mutase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,059,0220TC100.0% 800.9 / NA 236G51G (GGT→GGC) sbmmethylmalonyl‑CoA mutase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (107/129);  total (107/129)

Only 100 of 240 total aligned reads displayed.

TCGACTCGCTGGTTCATCAAACCGCGGAAGGGATCGCCATCAAGCCGCTGTATACCGAAGCCGATCTCGATAATCTGGAGGTGACAGGTACCCTTCCTGGTTTGCCGCCCTACGTTCGTGGCCCGCGTGCCACTATGTATACCGCCCAACCGTGGACCATCCGTCAGTATGCTGGTTT  >  NC_000913/3058934‑3059111
                                                                                        |                                                                                         
 cGACGCGCTGGTTCATCAAACCGCGGAAGGGAGCGCCATCAAGCCGCTGTATACCGAAGCCGATCTCGATAATCTGGAGGTGACAGGCACCCt                                                                                      <  2:5880353/93‑1 (MQ=255)
   aCTCGCTGGTTCATCAAACCGCGGAAGGGATCGCCATCAAGCCGCTGTATACCGAAGCCGATCTCGATAATCTGGAGGTGACAGGCACCCttg                                                                                    >  1:6206883/1‑92 (MQ=255)
   aCTCGCTGGTTCATCAAACCGCGGAAGGGATCGCCATCAAGCCGCTGTATACCGAAGCCGATCTCGATAATCTGGAGGTGACAGGCACCCTTc                                                                                    <  1:2370174/93‑1 (MQ=255)
    cTCGCTGGTTCATCAATCCGCGGAAGGAATCGCAATCAAGCCGCTGTATACCGAAGCCGATCTCGATAATCTGGAGGTGACAGGCACCCTTcc                                                                                   <  1:3903401/93‑1 (MQ=255)
    cTCGCTGGTTCATCAAACCGCGGAAGGGATCGCCATCAAGCCGCTGTATACCGAAGCCGATCTCGATAATCTGGAGGTGACAGGCACCCTTcc                                                                                   <  2:5268991/93‑1 (MQ=255)
     tCGCTGGTTCATCAAACCGCGGAAGGGATCGCCATCAAGCCGCTGTATACCGAAGCCGATCTCGATAATCTGGAGGTGACAGGCACCCTTCCt                                                                                  <  1:2467460/93‑1 (MQ=255)
     tCGCTGGTTCATCAAACCGCGGAAGGGATCGCCATCAAGCCGCTGTATACCGAAGCCGATCTCGATAATCTGGAGGTGACAGGCACCCTTCCt                                                                                  <  2:2292720/93‑1 (MQ=255)
       gCTGGTTCATCAAACCGCGGAAGGGATCGCCATCAAGCCGCTGTATACCGAAGCCGATCTCGATAATCTGGAGGTGACAGGCACCCTTCCTgg                                                                                <  2:1576637/93‑1 (MQ=255)
       gCTGGTTCATCAAACCGCGGAAGGGATCGCCATCAAGCCGCTGTATACCGAAGCCGATCTCGATAATCTGGAGGTGACAGGCACCCTTCCTgg                                                                                >  1:5724648/1‑93 (MQ=255)
         tGGTTCATCAAACCGCGGAAGGGATCGCCATCAAGCCGCTGTATACCGAAGCCGATCTCGATAATCTGGAGGTGACAGGCACCCTTCCTGGtt                                                                              >  1:5419534/1‑93 (MQ=255)
         tGGTTCATCAAACCGCGGAAGGGATCGCCATCAAGCCGCTGTATACCGAAGCCGATCTCGATAATCTGGAGGTGACAGGCACCCTTCCTGGtt                                                                              <  1:1714622/93‑1 (MQ=255)
         tGGTTCATCAAACCGCGGAAGGGATCGCCATCAAGCCGCTGTATACCGAAGCCGATCTCGATAATCTGGAGGTGACAGGCACCCTTCCTGGtt                                                                              <  2:4535603/93‑1 (MQ=255)
         tGGTTCATCAAACCGCGGAAGGGATCGCCATCAAGCCGCTGTATACCGAAGCCGATCTCGATAATCTGGAGGTGACAGGCACCCTTCCTGGtt                                                                              <  1:3283745/93‑1 (MQ=255)
          ggTTCATCAAACCGCGGAAGGGATCGCCATCAAGCCGCTGTATACCGAAGCCGATCTCGATAATCTGGAGGTGACAGGCACCCTTCCTGGttt                                                                             >  2:339536/1‑93 (MQ=255)
          ggTTCATCAAACCGCGGAAGGGATCGCCATCAAGCCGCTGTATACCGAAGCCGATCTCGATAATCTGGAGGTGACAGGCACCCTTCCTGGttt                                                                             <  1:59892/93‑1 (MQ=255)
          ggTTCATCAAACCGCGGAAGGGATCGCCATCAAGCCGCTGTATACCGAAGCCGATCTCGATAATCTGGAGGTGACAGGCACCCTTCCTGGttt                                                                             >  2:1732933/1‑93 (MQ=255)
          ggTTCATCAAACCGCGGAAGGGATCGCCATCAAGCCGCTGTATACCGAAGCCGATCTCGATAATCTGGAGGAGACAGGCACCCTTCCTGGttt                                                                             <  2:4110511/93‑1 (MQ=255)
           gTTCATCAAACCGCGGAAGGGATCGCCATCAAGCCGCTGTATACCGAAGCCGATCTCGATAATCTGGAGGTGACAGGCACCCTTCCTGGTTTg                                                                            <  1:5928659/93‑1 (MQ=255)
            ttCATCAAACCGCGGAAGGGATCGCCATCAAGCCGCTGTATACCGAAGCCGATCTCGATAATCTGGAGGTGACAGGCACCCTTCCTGGTTTgc                                                                           <  1:2860121/93‑1 (MQ=255)
             tcatcaAACCGCGGAAGGGATCGCCATCAAGCCGCTGTATACCGAAGCCGATCTCGATAATCTGGAGGTGACAGGCACCCTTCCTGGTTTgcc                                                                          <  1:1376006/93‑1 (MQ=255)
             taatcaACCCGCGGAAGGGATCGCGACCAAGCCGCTGTATACCGAAGCCGATCTCGATAATCTGGAGGTGACAGGCCCCCTTCCTGGTTTgcc                                                                          <  2:2807656/91‑1 (MQ=255)
               atcaAACCGCGGAAGGGATCGCCATCAAGCCGCTGTATACCGAAGCCGATCTCGATAATCTGGAGGTGACAGGCACCCTTCCTGGTTTgccgc                                                                        >  1:4348299/1‑93 (MQ=255)
               atcaAACCGCGGAAGGGATCGCCATCAAGCCGCTGTATACCGAAGCCGATCTCGATAATCTGGAGGTGACAGGCACCCTTCCTGGTTTgccgc                                                                        >  1:739670/1‑93 (MQ=255)
                tcaAACCGCGGAAGGGATCGCCATCAAGCCGCTGTATACCGAAGCCGATCTCGATAATCTGGAGGTGACAGGCACCCTTCCTGGTTTgccgcc                                                                       >  1:914694/1‑93 (MQ=255)
                 caAACCGGGGAAGGGATCGCCATCAAGCCGCTGTATACCGAAGCCGATCTCGATAATCTGGAGGTGACAGGCACCCTTCCTGCTTTGCCGccc                                                                      <  2:3949235/93‑1 (MQ=255)
                 caAACCGCGGAAGGGATCGCCATCAAGCCGCTGTATACCGAAGCCGATCTCGATAATCTGGAGGTGACAGGCACCCTTCCTGGTTTGCCGccc                                                                      >  1:2804536/1‑93 (MQ=255)
                    aCCGGGGAAGGGATCCCCATCAAGCCGCTGTATACCGAAGCCGATCTCGATAATCTGGAGGTGACAGGCACCCTTCCTGGTTTGCCGCCCTAc                                                                   <  2:1262154/93‑1 (MQ=255)
                       gcgGAAGGGATCGCCATCAAGCCGCTGTATACCGAAGCCGATCTCGATAATCTGGAGGTGACAGGCACCCTTCCTGGTTTGCCGCCCTACGtt                                                                <  2:2046920/93‑1 (MQ=255)
                       gcgGAAGGGATCGCCATCAAGCCGCTGTATACCGAAGCCGATCTCGATAATCTGGAGGTGACAGGCACCCTTCCTGGTTTGCCGCCCTACGtt                                                                <  2:1298206/93‑1 (MQ=255)
                          gAAGGGATCGCCATCAAGCCGCTGTATACCGAAGCCGATCTCGATAATCTGGAGGTGACAGGCACCCTTCCTGGTTTGCCGCCCTACGTTCGt                                                             <  1:5348190/93‑1 (MQ=255)
                          gAAGGGATCGCCATCAAGCCGCTGTATACCGAAGCCGATCTCGATAATCTGGAGGTGACAGGCACCCTTCCTGGTTTGCCGCCCTACGTTCGt                                                             <  1:522961/93‑1 (MQ=255)
                          gAAGGGATCGCCATCAAGCCGCTGTATACCGAAGCCGATCTCGATAATCTGGAGGTGACAGGCACCCTTCCTGGTTTGCCGCCCTACGTTCGt                                                             >  2:4804760/1‑93 (MQ=255)
                          gAAGGGATCGCCATCAAGCCGCTGTATACCGAAGCCGATCTCGATAATCTGGAGGTGACAGGCACCCTTCCTGGTTTGCCGCCCTACGTTCGt                                                             >  1:111744/1‑93 (MQ=255)
                          gAAGGGATCGCCATCAAGCCGCTGTATACCGAAGCCGATCTCGATAATCTGGAGGTGACAGGCACCCTTCCTGGTTTGCCGCCCTACGTTCGt                                                             >  2:2937968/1‑93 (MQ=255)
                          gAAGGGATCGCCATCAAGCCGCTGTATACCGAAGCCGATCTCGATAATCTGGAGGTGACAGGCACCCTTCCTGGTTTGCCGCCCTACGTTCGt                                                             <  1:4120876/93‑1 (MQ=255)
                           aaGGGATCGCCATCAAGCCGCTGTATACCGAAGCCGATCTCGATAATCTGGAGGTGACAGGCACCCTTCCTGGTTTGCCGCCCTACGTTCGTg                                                            <  2:4268322/93‑1 (MQ=255)
                             gggATCGCCATCAAGCCGCTGTATACCGAAGCCGATCTCGATAATCTGGAGGTGACAGGCACCCTTCCTGGTTTGCCGCCCTACGTTCGTGGc                                                          <  1:6166463/93‑1 (MQ=255)
                               aaTCGCCATCAAGCCGCTGTATACCGAAGCCGATCTCGATAATCTGGAGGTGACAGGCACCCTTCCTGGTTTGCCGCCCTACGTTCGTGGccc                                                        <  1:3448737/92‑1 (MQ=255)
                                 tCGCCATCAAGCCGCTGTATACCGAAGCCGATCTCGATAATCTGGAGGTGACAGGCACCCTTCCTGGTTTGCCGCCCTACGTTCGTGGCCcgc                                                      <  1:2533255/93‑1 (MQ=255)
                                  cGCCATCAAGCCGCTGTATACCGAAGCCGATCTCGATAATCTGGAGGTGACAGGCACCCTTCCTGGTTTGCCGCCCTACGTTCGTGGCCcgcg                                                     <  2:4705721/93‑1 (MQ=255)
                                   gCCATCAAGCCGCTGTATACCGAAGCCGATCTCGATAATCTGGAGGTGACAGGCACCCTTCCTGGTTTGCCGCCCTACGTTCGTGGCCCGCGt                                                    <  1:156423/93‑1 (MQ=255)
                                   gCCATCAAGCCGCTGTATACCGAAGCCGATCTCGATAATCTGGAGGTGACAGGCACCCTTCCTGGTTTGCCGCCCTACGTTCGTGGCCCGCGt                                                    <  2:1840538/93‑1 (MQ=255)
                                     cATCAAGCCGCTGTATACCGAAGCCGATCTCGATAATCTGGAGGTGACAGGCACCCTTCCTGGTTTGCCGCCCTACGTTCGTGGCCCGCGTGc                                                  >  1:3615584/1‑93 (MQ=255)
                                          aGCCGCTGTATACCGAAGCCGATCTCGATAATCTGGAGGTGACAGGCACCCTTCCTGGTTTGCCGCCCTACGTTCGTGGCCCGCGTGCCACTa                                             >  2:3922299/1‑93 (MQ=255)
                                          aGCCGCTGTATACCGAAGCCGATCTCGATAATCTGGAGGTGACAGGCACCCTTCCTGGTTTGCCGCCCTACGTTCGTGGCCCGCGTGCCACTa                                             <  2:5404031/93‑1 (MQ=255)
                                          aGCCGCTGTATACCGAAGCCGATCTCGATAATCTGGAGGTGACAGGCACCCTTCCTGGTTTGCCGCCCTACGTTCGTGGCCCGCGTGCCACTa                                             <  1:1900089/93‑1 (MQ=255)
                                           gCCGCTGTATACCGAAGCCGATCTCGATAATCTGGAGGTGACAGGCACCCTTCCTGGTTTGCCGCCCTACGTTCGTGGCCCGCGTGCCACTAt                                            >  2:6120405/1‑93 (MQ=255)
                                           gCCGCTGTATACCGAAGCCGATCTCGATAATCTGGAGGTGACAGGCACCCTTCCTGGTTTGCCGCCCTACGTTCGTGGCCCGCGTGCCACTAt                                            <  1:3488624/93‑1 (MQ=255)
                                           gCCGCTGTATACCGAAGCCGATCTCGATAATCTGGAGGTGACAGGCACCCTTCCTGGTTTGCCGCCCTACGTTCGTGGCCCGCGTGCCACTAt                                            >  1:429973/1‑93 (MQ=255)
                                             cGCTGTATACCGAAGCCGATCTCGATAATCTGGAGGTGACAGGCACCCTTCCTGGTTTGCCGCCCTACGTTCGTGGCCCGCGTGCCACTATGt                                          <  2:6447080/93‑1 (MQ=255)
                                              gCTGTATACCGAAGCCGATCTCGATAATCTGGAGGTGACAGGCACCCTTCCTGGTTTGCCGCCCTACGTTCGTGGCCCGCGTGCCACTATGta                                         <  1:1018862/93‑1 (MQ=255)
                                              gCTGTATACCGAAGCCGATCTCGATAATCTGGAGGTGACAGGCACCCTTCCTGGTTTGCCGCCCTACGTTCGTGGCCCGCGTGCCACTATGta                                         >  1:518565/1‑93 (MQ=255)
                                                tGTATACCTAAGCCGATCTCGATAATCTGGAGGTGACAGGCACCCTTCCTGGTTTGCCGCCCTACGTTCGTGGCCCGCGTGCCACTATGtata                                       >  2:6319364/1‑93 (MQ=255)
                                                 gTATACCGAAGCCGATCTCGATAATCTGGAGGTGACAGGCACCCTTCCTGGTTTGCCGCCCTACGTTCGTGGCCCGCGTGCCACTATGTATAc                                      >  1:1321307/1‑93 (MQ=255)
                                                 gTATACCGAAGCCGATCTCGATAATCTGGAGGTGACAGGCACCCTTCCTGGTTTGCCGCCCTACGTTCGTGGCCCGCGTGCCACTATGTATAc                                      >  1:5013660/1‑93 (MQ=255)
                                                  tataCCGAAGCCGATCTCGATAATCTGGAGGTGACAGGCACCCTTCCTGGTTTGCCGCCCTACGTTCGTGGCCCGCGTGCCACTATGTATAcc                                     <  2:1184864/93‑1 (MQ=255)
                                                  tataCCGAAGCCGATCTCGATAATCTGGAGGTGACAGGCACCCTTCCTGGTTTGCCGCCCTACGTTCGTGGCCCGCGTGCCACTATGTATAcc                                     >  1:2765546/1‑93 (MQ=255)
                                                    taCCGAAGCCGATCTCGATAATCTGGAGGTGACAGGCACCCTTCCTGGTTTGCCGCCCTACGTTCGTGGCCCGCGTGCCACTATGTATACCGc                                   <  1:875723/93‑1 (MQ=255)
                                                      ccGAAGCCGATCTCGATAATCTGGAGGTGACAGGCACCCTTCCTGGTTTGCCGCCCTACGTTCGTGGCCCGCGTGCCACTATGTATACCGccc                                 >  2:2127487/1‑93 (MQ=255)
                                                       cGAAGCCGATCTCGATAATCTGGAGGTGCCAGGCACCCTTCCTGGTTTGCCGCCCTCCGTTCGTGGCCCGCGTGCCACTATGTATCCCGCCCa                                <  1:2317064/93‑1 (MQ=255)
                                                        gAAGCCGATCTCGATAATCTGGAGGTGACAGGCGCCCTTCCTGGTTTGCCGCCCTACGTTCGTGGCCCGCGTGCCACTATGTATACCGCCCaa                               <  1:2448316/93‑1 (MQ=255)
                                                        gAAGCCGATCTCGATAATCTGGAGGTGACAGGCACCCTTCCTGGTTTGCCGCCCTACGTTCGTGGCCCGCGTGCCACTATGTATACCGCCCaa                               >  1:31336/1‑93 (MQ=255)
                                                         aaGCCGATCTCGATAATCTGGAGGTGACAGGCACCCTTCCTGGTTTGCCGCCCTACGTTCGTGGCCCGCGTGCCACTATGTATACCGCCCAAc                              <  2:4646519/93‑1 (MQ=255)
                                                         aaGCCGATCTCGATAATCTGGAGGTGACAGGCACCCTTCCTGGTTTGCCGCCCTACGTTCGTGGCCCGCGTGCCACTATGTATACCGCCCAAc                              >  1:25022/1‑93 (MQ=255)
                                                         aaGCCGATCTCGATAATCTGGAGGTGACAGGCACCCTTCCTGGTTTGCCGCCCTACGTTCGTGGCCCGCGTGCCACTATGTATACCGCCCAAc                              <  1:2241406/93‑1 (MQ=255)
                                                           gCCGATCTCGATAATCTGGAGGTGACAGGCACCCTTCCTGGTTTGCCGCCCTACGTTCGTGGCCCGCGTGCCACTATGTATACCGCCCAACCg                            >  1:6094277/1‑93 (MQ=255)
                                                             cGATCTCGATAATCTGGAGGTGACAGGCACCCTTCCTGGTTTGCCGCCCTACGTTCGTGGCCCGCGTGCCACTATGTATACCGCCCAACCGTg                          >  2:823786/1‑93 (MQ=255)
                                                             cGATCTCGATAATCTGGAGGTGACAGGCACCCTTCCTGGTTTGCCGCCCTACGTTCGTGGCCCGCGTGCCACTATGTATACCGCCCAACCGTg                          >  1:4506997/1‑93 (MQ=255)
                                                              gATCTCGATAATCTGGAGGTGACAGGCACCCTTCCTGGTTTGCCGCCCTACGTTCGTGGCCCGCGTGCCACTATGTATACCGCCCAACCGTgg                         >  2:919146/1‑93 (MQ=255)
                                                                tctcGATAATCTGGAGGTGACAGGCACCCTTCCTGGCTTGCCGCCCTACGTTCGTGGCCCGCGTGCCACTATGTATACCGCCCAACCGTGGAc                       >  2:3243661/1‑93 (MQ=255)
                                                                  tcGATAATCTGGAGGTGACAGGCACCCTTCCTGGTTTGCCGCCCTACGTTCGTGGCCCGCGTGCCACTATGTATACCGCCCAACCGTGGACCa                     >  1:1635006/1‑93 (MQ=255)
                                                                      tAATCTGGAGGTGACAGGCACCCTTCCTGGTTTGCCGCCCTACGTTCGTGGCCCGCGTGCCACTATGTATACCGCCCAACCGTGGACCATCCg                 <  2:3128515/93‑1 (MQ=255)
                                                                       aaTCTGGAGGTGACAGGCACCCTTCCTGGTTTGCCGCCCTACGTTCGTGGCCCGCGTGCCACTATGTATACCGCCCAACCGTGGACCATCCGt                >  1:5693625/1‑93 (MQ=255)
                                                                        aTCTGGAGGTGACAGGCACCCTTCCTGGTTTGCCGCCCTACGTTCGTGGCCCGCGTGCCACTATGTATACCGCCCAACCGTGGACCATCCGTc               >  1:3993775/1‑93 (MQ=255)
                                                                        aTCTGGAGGTGACAGGCACCCTTCCTGGTTTGCCGCCCTACGTTCGTGGCCCGCGTGCCACTATGTATACCGCCCAACCGTGGACCATCCGTc               <  1:6431432/93‑1 (MQ=255)
                                                                        aTCTGGAGGTGACAGGCACCCTTCCTGGTTTGCCGCCCTACGTTCGTGGCCCGCGTGCCACTATGTATACCGCCCAACCGTGGACCATCCGTc               >  1:1191138/1‑93 (MQ=255)
                                                                        aTCTGGAGGTGACAGGCACCCTTCCTGGTTTGCCGCCCTACGTTCGTGGCCCGCGTGCCACTATGTATACCGCCCAACCGTGGACCATCCGTc               >  1:6471474/1‑93 (MQ=255)
                                                                        aTCTGGAGGTGACAGGCACCCTTCCTGGTTTGCCGCCCTACGTTCGTGGCCCGCGTGCCACTATGTATACCGCCCAACCGTGGACCATCCGTc               >  1:3024044/1‑93 (MQ=255)
                                                                         tCTGGAGGTGACAGGCACCCTTCCTGGTTTGCCGCCCTACGTTCGTGGCCCGCGTGCCACTATGTATACCGCCCAACCGTGGACCATCCGTCa              <  2:2585862/93‑1 (MQ=255)
                                                                         tCTGGAGGTGACAGGCACCCTTCCTGGTTTGCCGCCCTACGTTCGTGGCCCGCGTGCCACTATGTATACCGCCCAACCGTGGACCATCCGTCa              <  2:1993280/93‑1 (MQ=255)
                                                                           tGGAGGTGACAGGCACCCTTCCTGGTTTGCCGCCCTACGTTCGTGGCCCGCGTGCCACTATGTATACCGCCCAACCGTGGACCATCCGTCAGt            >  1:999559/1‑93 (MQ=255)
                                                                           tGGAGGTGACAGGCACCCTTCCTGGTTTGCCGCCCTACGTTCGTGGCCCGCATGCCACTCTGTATACCGCCCAACCGTGGACCATCCGTCAga            >  2:1829367/1‑92 (MQ=255)
                                                                            ggAGGTGACAGGCACCCTTCCTGGTTTGCCGCCCTACGTTCGTGGCCCGCGTGCCACTATGTATACCGCCCAACCGTGGACCATCCGTCAGTa           <  1:4698348/93‑1 (MQ=255)
                                                                            ggAGGTGACAGGCACCCTTCCTGGTTTGCCGCCCTACGTTCGTGGCCCGCGTGCCACTATGTATACCGCCCAACCGTGGACCATCCGTCAGTa           <  1:5584189/93‑1 (MQ=255)
                                                                            ggAGGTGACAGGCACCCTTCCTGGTTTGCCGCCCTACGTTCGTGGCCCGCGTGCCACTATGTATACCGCCCAACCGTGGACCATCCGTCAGTa           <  2:3639179/93‑1 (MQ=255)
                                                                            ggAGGTGACAGGCACCCTTCCTGGTTTGCCGCCCTACGTTCGTGGCCCGCGTGCCACTATGTATACCGCCCAACCGTGGACCATCCGTCAGTa           <  2:5695764/93‑1 (MQ=255)
                                                                            ggAGCTGACAGGAACCCTTCCTGGTTTGCCGCCCTACGTTCGTGGCCCGCGTGCCACTATGTATACCGCCCAACCGTGGACCATCCGTCAGTa           <  2:6085212/93‑1 (MQ=255)
                                                                             gAGGTGACAGCCACCCTTCCTGGTTTGCCGCCCTACGTTCGTGGCCCGCGTGCCACTATGTATACCGCCCAACCGTGGACCATCCGTCAGTAt          <  2:5941879/93‑1 (MQ=255)
                                                                              aGGTGACAGGCACCCTTCCTGGTTTGCCGCCCTACGTTCGTGGCCCGCGTGCCACTATGTATACCGCCCAACCGTGGACCATCCGTCAGTATg         <  2:3736276/93‑1 (MQ=255)
                                                                              aGGTGACAGGCACCCTTCCTGGTTTGCCGCCCTACGTTCGTGGCCCGCGTGCCACTATGTATACCGCCCAACCGTGGACCATCCGTCAGTATg         <  2:5045432/93‑1 (MQ=255)
                                                                               ggTGACAGGCCCCCTTCCTGGTTTGCCGCCCTACGTTCGTGGCCCGCGTGCCACTATGTATACCGCCCAACCGTGGACCATCCGTCAGTATGc        <  1:3549609/93‑1 (MQ=255)
                                                                               ggTGACAGGCACCCTTCCTGGTTTGCCGCCCTACGTTCGTGGCCCGCGTGCCACTATGTATACCGCCCAACCGTGGACCATCCGTCAGTATGc        <  1:170019/93‑1 (MQ=255)
                                                                               ggTGACAGGCACCCTTCCTGGTTTGCCGCCCTACGTTCGTGGCCCGCGTGCCACTATGTATACCGCCCAACCGTGGACCATCCGTCAGTATGc        <  2:3314512/93‑1 (MQ=255)
                                                                                 tGACAGGCACCCTTCCTGGTTTGCCGCCCTACGTTCGTGGCCCGCGTGCCACTATGTATACCGCCCAACCGTGGACCATCCGTCAGTATGCTg      >  2:5490012/1‑93 (MQ=255)
                                                                                 ggACAGGCCCCCTTCCTGGTTTGCCGCCCTACGTTCGTGGCCCGCGTGCCACTATGTATACCGCCCAACCGTGGACCATCCGTCAGTATGCTg      <  2:2198256/92‑1 (MQ=255)
                                                                                  gACAGGCACCCTTCCTGGTTTGCCGCCCTACGTTCGTGGCCCGCGTGCCACTATGTATACCGCCCAACCGTGGACCATCCGTCAGTATGCTgg     <  1:3643077/93‑1 (MQ=255)
                                                                                  gACAGGCACCCTTCCTGGTTTGCCGCCCTACGTTCGTGGCCCGCGTGCCACTATGTATACCGCCCAACCGTGGACCATCCGTCAGTATGCTgg     <  2:1221051/93‑1 (MQ=255)
                                                                                   aCAGGCACCCTTCCTGGTTTGCCGCCCTACGTTCGTGGCCCGCGTGCCACTATGTATACCGCCCAACCGTGGACCATCCGTCAGTATGCTGGt    <  2:3433308/93‑1 (MQ=255)
                                                                                   aCAGGCACCCTTCCTGGTTTGCCGCCCTACGTTCGTGGCCCGCGTGCCACTATGTATACCGCCCAACCGTGGACCATCCGTCAGTATGCTGGt    <  2:3676783/93‑1 (MQ=255)
                                                                                    cAGGCACCCTTCCTGGTTTGCCGCCCTACGTTCGTGGCCCGCGTGCCACTATGTATACCGCCCAACCGTGGACCATCCGTCAGTATGCTGGtt   <  2:5108383/93‑1 (MQ=255)
                                                                                        |                                                                                         
TCGACTCGCTGGTTCATCAAACCGCGGAAGGGATCGCCATCAAGCCGCTGTATACCGAAGCCGATCTCGATAATCTGGAGGTGACAGGTACCCTTCCTGGTTTGCCGCCCTACGTTCGTGGCCCGCGTGCCACTATGTATACCGCCCAACCGTGGACCATCCGTCAGTATGCTGGTTT  >  NC_000913/3058934‑3059111

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 19 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
AACTGTCGACTCGCTGGTTCATCAAACCGCGGAAGGGATCGCCATCAAGCCGCTGTATACCGAAGCCGATCTCGATAATCTGGAGGTGACAGGTACCCTTCCTGGTTTGCCGCCCTACGTTCGTGGCCCGCGTGCCACTATGTATACCGCCCAACCGTGGACCATCCGTCAGTATGCTGGTTTTTCA  >  NC_000913/3058929‑3059115
                                                                                             |                                                                                             
AACTGTCGACTCGCTGGTTCATCAAACCGCGGAAGGGATCGCCATCAAGCCGCTGTATACCGAAGCCGATCTCGATAATCTGGAGGTGACAGGC                                                                                               <  SRR4302537.3018185/94‑1 (MQ=60)
 ACTTTCGCCTCTCTGGTTCATCAATCCGCGGAAGGGATCGCCATCAAGCCGCTGTATACCGAAGCCGATCTCGATAATCTGGAGGTGACAGGCA                                                                                              <  SRR4302537.544930/94‑1 (MQ=60)
 ACTGTCGACTCGCTGGTTCATCAAACCGCGGAAGGGATCGCCATCAAGCCGCTGTATACCGAAGCCGATCTCGATAATCTGGAGGTGACAGGCA                                                                                              >  SRR4302537.283275/1‑94 (MQ=60)
 ACTGTCGACTCGCTGGTTCATCAAACCGCGGAAGGGATCGCCATCAAGCCGCTGTATACCGAAGCCGATCTCGATAATCTGGAGGTGACAGGCA                                                                                              >  SRR4302537.5220030/1‑94 (MQ=60)
  CTGTCGACTCGCTGGTTCATCAAACCGCGGAAGGGATCGCCATCAAGCCGCTGTATACCGAAGCCGATCTCGATAATCTGGAGGTGACAGGCAC                                                                                             <  SRR4302537.884705/94‑1 (MQ=60)
      CGACTCGCTGGTTCATCAAACCGCGGAAGGGATCGCCATCAAGCCGCTGTATACCGAAGCCGATCTCGATAATCTGGAGGTGACAGGCACCCTT                                                                                         >  SRR4302537.1853770/1‑94 (MQ=60)
      CGACTCGCTGGTTCATCAAACCGCGGAAGGGATCGCCATCAAGCCGCTGTATACCGAAGCCGATCTCGATAATCTGGAGGTGACAGGCACCCTT                                                                                         >  SRR4302537.5209157/1‑94 (MQ=60)
       GACTCGCTGGTTCATCAAACCGCGGAAGGGATCGCCATCAAGCCGCTGTATACCGAAGCCGATCTCGATAATCTGGAGGTGACAGGCACCCTTG                                                                                        >  SRR4302537.6249652/1‑94 (MQ=60)
       GACTCGCTGGTTCATCAAACCGCGGAAGGGATCGCCATCAAGCCGCTGTATACCGAAGCCGATCTCGATAATCTGGAGGTGACAGGCACCCTTC                                                                                        >  SRR4302537.3215043/1‑94 (MQ=60)
        ACTCGCTGGTTCATCAAACCGCGGAAGGGATCGCCATCAAGCCGCTGTATACCGAAGCCGATCTCGATAATCTGGAGGTGACAGGCACCCTTCC                                                                                       <  SRR4302537.2386876/94‑1 (MQ=60)
        ACTCGCTGGTTCATCAAACCGCGGAAGGGATCGCCATCAAGCCGCTGTATACCGAAGCCGATCTCGATAATCTGGAGGTGACAGGCACCCTTCC                                                                                       <  SRR4302537.2468052/94‑1 (MQ=60)
        ACTCGCTGGTTCATCAAACCGCGGAAGGGATCGCCATCAAGCCGCTGTATACCGAAGCCGATCTCGATAATCTGGAGGTGACAGGCACCCTTCC                                                                                       <  SRR4302537.2888867/94‑1 (MQ=60)
         CTCGCTGGTTCATCAATCCGCGGAAGGAATCGCAATCAAGCCGCTGTATACCGAAGCCGATCTCGATAATCTGGAGGTGACAGGCACCCTTCCT                                                                                      <  SRR4302537.3930688/94‑1 (MQ=60)
         CTCGCTGGTTCATCAAACCGCGGAAGGGATCGCCATCAAGCCGCTGTATACCGAAGCCGATCTCGATAATCTGGAGGTGACAGGCACCCTTCCT                                                                                      >  SRR4302537.625441/1‑94 (MQ=60)
         CTCGCTGGTTCATCAAACCGCGGAAGGGATCGCCATCAAGCCGCTGTATACCGAAGCCGATCTCGATAATCTGGAGGTGACAGGCACCCTTCCT                                                                                      <  SRR4302537.1358575/94‑1 (MQ=60)
         CTCGCTGGTTCATCAAACCGCGGAAGGGATCGCCATCAAGCCGCTGTATACCGAAGCCGATCTCGATAATCTGGAGGTGACAGGCACCCTTCCT                                                                                      <  SRR4302537.2072322/94‑1 (MQ=60)
          tcgcgggtcaacaaaccggcgaAAGGGATCGCCATCAATGCGCTGTATACCGAAGCCGATCTCGATAATCTGGAGGTGACAGGCACCCTTCCTG                                                                                     <  SRR4302537.786176/72‑1 (MQ=60)
          TCGCTGGTTCATCAAACCGCGGAAGGGATCGCCATCAAGCCGCTGTATACCGAAGCCGATCTCGATAATCTGGAGGTGACAGGCACCCTTCCTG                                                                                     <  SRR4302537.2484807/94‑1 (MQ=60)
          CCGCTGGTTCATCAAACCGCGGAAGGGATCGCCATCAAGCCGCTGTATACCGAAGCCGATCTCGATAATCTGGAGGTGACAGGCACCCTTCCTG                                                                                     <  SRR4302537.5361239/94‑1 (MQ=60)
           CGCTGGTTCATCAAACCGCGGAAGGGATCGCCATCAAGCCGCTGTATACCGAAGCCGATCTCGATAATCTGGAGGTGACAGGCACCCTTCCTGG                                                                                    >  SRR4302537.5763834/1‑94 (MQ=60)
           CGCGGGTTCATCAAACCGCGGAAGGGATCGCCATCAAGCCGCTGTATACCGAAGCCGATCTCGATAATCTGGAGGTGACAGGCACCCTTCCTGG                                                                                    <  SRR4302537.2982834/94‑1 (MQ=60)
            GCTGGTTCATCAAACCGCGGAAGGGATCGCCATCAAGCCGCTGTATACCGAAGCCGATCTCGATAATCTGGAGGTGACAGGCACCCTTCCTGGT                                                                                   >  SRR4302537.5509325/1‑94 (MQ=60)
            GCTGGTTCATCAAACCGCGGAAGGGATCGCCATCAAGCCGCTGTATACCGAAGCCGATCTCGATAATCTGGAGGTGACAGGCACCCTTCCTGGT                                                                                   >  SRR4302537.3053698/1‑94 (MQ=60)
             CTGGTTCATCAAACCGCGGAAGGGATCGCCATCAAGCCGCTGTATACCGAAGCCGATCTCGATAATCTGGAGGTGACAGGCACCCTTCCTGGTT                                                                                  >  SRR4302537.5456468/1‑94 (MQ=60)
              TGGTTCATCAAACCGCGGAAGGGATCGCCATCAAGCCGCTGTATACCGAAGCCGATCTCGATAATCTGGAGGTGACAGGCACCCTTCCTGGTTT                                                                                 <  SRR4302537.1726592/94‑1 (MQ=60)
              TGGTTCATCAAACCGCGGAAGGGATCGCCATCAAGCCGCTGTATACCGAAGCCGATCTCGATAATCTGGAGGTGACAGGCACCCTTCCTGGTTT                                                                                 >  SRR4302537.1700334/1‑94 (MQ=60)
              TGGTTCATCAAACCGCGGAAGGGATCGCCATCAAGCCGCTGTATACCGAAGCCGATCTCGATAATCTGGAGGTGACAGGCACCCTTCCTGGTTT                                                                                 >  SRR4302537.2728026/1‑94 (MQ=60)
              TGGTTCATCAAACCGCGGAAGGGATCGCCATCAAGCCGCTGTATACCGAAGCCGATCTCGATAATCTGGAGGTGACAGGCACCCTTCCTGGTTT                                                                                 <  SRR4302537.5959293/94‑1 (MQ=60)
              TGGTTCATCAAACCGCGGAAGGGATCGCCATCAAGCCGCTGTATACCGAAGCCGATCTCGATAATCTGGAGGTGACAGGCACCCTTCCTGGTTT                                                                                 <  SRR4302537.3306929/94‑1 (MQ=60)
               GGTTCATCAAACCGCGGAAGGGATCGCCATCAAGCCGCTGTATACCGAAGCCGATCTCGATAATCTGGAGGTGACAGGCACCCTTCCTGGTTTG                                                                                <  SRR4302537.60337/94‑1 (MQ=60)
               GGTTCATCAAACCGCGGAAGGGATCGCCATCAAGCCGCTGTATACCGAAGCCGATCTCGATAATCTGGAGGTGACAGGCACCCTTCCTGGTTTC                                                                                >  SRR4302537.565788/1‑94 (MQ=60)
                GTTCATCAAACCGCGGAAGGGATCGCCATCAAGCCGCTGTATACCGAAGCCGATCTCGATAATCTGGAGGTGACAGGCACCCTTCCTGGTTTGC                                                                               <  SRR4302537.5969343/94‑1 (MQ=60)
                 TTCATCAAACCGCGGAAGGGATCGCCATCAAGCCGCTGTATACCGAAGCCGATCTCGATAATCTGGAGGTGACAGGCACCCTTCCTGGTTTGCC                                                                              <  SRR4302537.2880175/94‑1 (MQ=60)
                 TTCATCAAACCGCGGAAGGGATCGCCATCAAGCCGCTGTATACCGAAGCCGATCTCGATAATCTGGAGGTGACAGGCACCCTTCCTGGTTTGCC                                                                              >  SRR4302537.4854103/1‑94 (MQ=60)
                  TCATCAAACCGCGGAAGGGATCGCCATCAAGCCGCTGTATACCGAAGCCGATCTCGATAATCTGGAGGTGACAGGCACCCTTCCTGGTTTGCCG                                                                             <  SRR4302537.1385611/94‑1 (MQ=60)
                   CATCAAACCGCGGAAGGGATCGCCATCAAGCCGCTGTATACCGAAGCCGATCTCGATAATCTGGAGGTGACAGGCACCCTTCCTGGTTTGCCGC                                                                            >  SRR4302537.4378433/1‑94 (MQ=60)
                   CATCAAACCGCGGAAGGGATCGCCATCAAGCCGCTGTATACCGAAGCCGATCTCGATAATCTGGAGGTGACAGGCACCCTTCCTGGTTTGCCGC                                                                            >  SRR4302537.744713/1‑94 (MQ=60)
                    ATCAAACCGCGGAAGGGATCGCCATCAAGCCGCTGTATACCGAAGCCGATCTCGATAATCTGGAGGTGACAGGCACCCTTCCTGGTTTGCCGCC                                                                           >  SRR4302537.921008/1‑94 (MQ=60)
                    ATCAAACCGCGGAAGGGATCGCCATCAAGCCGCTGTATACCGAAGCCGATCTCGATAATCTGGAGGTGACAGGCACCCTTCCTGGTTTGCCGCC                                                                           >  SRR4302537.5734737/1‑94 (MQ=60)
                    ATCAAACCGCGGAAGGGATCGCCATCAAGCCGCTGTATACCGAAGCCGATCTCGATAATCTGGAGGTGACAGGCACCCTTCCTGGTTTGCCGCC                                                                           >  SRR4302537.3849138/1‑94 (MQ=60)
                     TCAAACCGCGGAAGGGATCGCCATCAAGCCGCTGTATACCGAAGCCGATCTCGATAATCTGGAGGTGACAGGCACCCTTCCTGGTTTGCCGCCC                                                                          >  SRR4302537.2824137/1‑94 (MQ=60)
                      CAAACCGCGGAAGGGATCGCCATCAAGCCGCTGTATACCGAAGCCGATCTCGATAATCTGGAGGTGACAGGCACCCTTCCTGGTTTGCCGCCCT                                                                         <  SRR4302537.6167907/94‑1 (MQ=60)
                        AACCGCGGAAGGGATCGCCATCAAGCCGCTGTATACCGAAGCCGATCTCGATAATCTGGAGGTGACAGGCACCCTTCCTGGTTTGCCGCCCTAC                                                                       <  SRR4302537.437353/94‑1 (MQ=60)
                          CCGCGGAAGGGATCGCCATCAAGCCGCTGTATACCGAAGCCGATCTCGATAATCTGGAGGTGACAGGCACCCTTCCTGGTTTGCCGCCCTACGT                                                                     <  SRR4302537.92179/94‑1 (MQ=60)
                           CGCGGAAGGGATCGCCATCAAGCCGCTGTATACCGAAGCCGATCTCGATAATCTGGAGGTGACAGGCACCCTTCCTGGTTTGCCGCCCTACGTT                                                                    >  SRR4302537.254022/1‑94 (MQ=60)
                           CGCGGAAGGGATCGCCATCAAGCCGCTGTATACCGAAGCCGATCTCGATAATCTGGAGGTGACAGGCACCCTTCCTGGTTTGCCGCCCTACGTT                                                                    <  SRR4302537.1720053/94‑1 (MQ=60)
                            GCGGAAGGGATCGCCATCAAGCCGCTGTATACCGAAGCCGATCTCGATAATCTGGAGGTGACAGGCACCCTTCCTGGTTTGCCGCCCTACGTTC                                                                   <  SRR4302537.1603178/94‑1 (MQ=60)
                            GCGGAAGGGATCGCCATCAAGCCGCTGTATACCGAAGCCGATCTCGATAATCTGGAGGTGACAGGCACCCTTCCTGGTTTGCCGCCCTACGTTC                                                                   <  SRR4302537.126907/94‑1 (MQ=60)
                            GCGGAAGGGATCGCCATCAAGCCGCTGTATACCGAAGCCGATCTCGATAATCTGGAGGTGACAGGCACCCTTCCTGGTTTGCCGCCCTACGTTC                                                                   <  SRR4302537.2311187/94‑1 (MQ=60)
                              GGAAGGGATCGCCATCAAGCCGCTGTATACCGAAGCCGATCTCGATAATCTGGAGGTGACAGGCACCCTTCCTGGTTTGCCGCCCTACGTTCGT                                                                 >  SRR4302537.112562/1‑94 (MQ=60)
                               GAAGGGATCGCCATCAAGCCGCTGTATACCGAAGCCGATCTCGATAATCTGGAGGTGACAGGCACCCTTCCTGGTTTGCCGCCCTACGTTCGTG                                                                <  SRR4302537.526550/94‑1 (MQ=60)
                               GAAGGGATCGCCATCAAGCCGCTGTATACCGAAGCCGATCTCGATAATCTGGAGGTGACAGGCACCCTTCCTGGTTTGCCGCCCTACGTTCGTG                                                                <  SRR4302537.4149648/94‑1 (MQ=60)
                               GAAGGGATCGCCATCAAGCCGCTGTATACCGAAGCCGATCTCGATAATCTGGAGGTGACAGGCACCCTTCCTGGTTTGCCGCCCTACGTTCGTG                                                                <  SRR4302537.5384835/94‑1 (MQ=60)
                                  GGGATCGCCATCAAGCCGCTGTATACCGAAGCCGATCTCGATAATCTGGAGGTGACAGGCACCCTTCCTGGTTTGCCGCCCTACGTTCGTGGCC                                                             <  SRR4302537.2807602/94‑1 (MQ=60)
                                  GGGATCGCCATCAAGCCGCTGTATACCGAAGCCGATCTCGATAATCTGGAGGTGACAGGCACCCTTCCTGGTTTGCCGCCCTACGTTCGTGGCC                                                             <  SRR4302537.6208909/94‑1 (MQ=60)
                                  GGGATCGCCATCAAGCCGCTGTATACCGAAGCCGATCTCGATAATCTGGAGGTGACAGGCACCCTTCCTGGTTTGCCGCCCTACGTTCGTGGCC                                                             <  SRR4302537.1714456/94‑1 (MQ=60)
                                  GGGATCGCCATCAAGCCGCTGTATACCGAAGCCGATCTCGATAATCTGGAGGTGACAGGCACCCTTCCTGGTTTGCCGCCCTACGTTCGTGGCC                                                             <  SRR4302537.5552712/94‑1 (MQ=60)
                                    AATCGCCATCAAGCCGCTGTATACCGAAGCCGATCTCGATAATCTGGAGGTGACAGGCACCCTTCCTGGTTTGCCGCCCTACGTTCGTGGCCCG                                                           <  SRR4302537.3473133/94‑1 (MQ=60)
                                     ATCGCCATCAAGCCGCTGTATACCGAAGCCGATCTCGATAATCTGGAGGTGACAGGCACCCTTCCTGGTTTGCCGCCCTACGTTCGTGGCCCGC                                                          >  SRR4302537.6229970/1‑94 (MQ=60)
                                     ATCGCCATCAAGCCGCTGTATACCGAAGCCGATCTCGATAATCTGGAGGTGACAGGCACCCTTCCTGGTTTGCCGCCCTACGTTCGTGGCCCGC                                                          >  SRR4302537.3654422/1‑94 (MQ=60)
                                      TCGCCATCAAGCCGCTGTATACCGAAGCCGATCTCGATAATCTGGAGGTGACAGGCACCCTTCCTGGTTTGCCGCCCTACGTTCGTGGCCCGCG                                                         <  SRR4302537.696790/94‑1 (MQ=60)
                                      TCGCCATCAAGCCGCTGTATACCGAAGCCGATCTCGATAATCTGGAGGTGACAGGCACCCTTCCTGGTTTGCCGCCCTACGTTCGTGGCCCGCG                                                         <  SRR4302537.2551032/94‑1 (MQ=60)
                                        GCCATCAAGCCGCTGTATACCGAAGCCGATCTCGATAATCTGGAGGTGACAGGCACCCTTCCTGGTTTGCCGCCCTACGTTCGTGGCCCGCGTG                                                       <  SRR4302537.157537/94‑1 (MQ=60)
                                        GCCATCAAGCCGCTGTATACCGAAGCCGATCTCGATAATCTGGAGGTGACAGGCACCCTTCCTGGTTTGCCGCCCTACGTTCGTGGCCCGCGTG                                                       >  SRR4302537.2796272/1‑94 (MQ=60)
                                        GCCATCAAGCCGCTGTATACCGAAGCCGATCTCGATAATCTGGAGGTGACAGGCACCCTTCCTGGTTTGCCGCCCTACGTTCGTGGCCCGCGTG                                                       >  SRR4302537.417021/1‑94 (MQ=60)
                                         CCATCAAGCCGCTGTATACCGAAGCCGATCTCGATAATCTGGAGGTGACAGGCACCCTTCCTGGTTTGCCGCCCTACGTTCGTGGCCCGCGTGC                                                      >  SRR4302537.3641074/1‑94 (MQ=60)
                                         CCATCAAGCCGCTGTATACCGAAGCCGATCTCGATAATCTGGAGGTGACAGGCACCCTTCCTGGTTTGCCGCCCTACGTTCGTGGCCCGCGTGC                                                      >  SRR4302537.374915/1‑94 (MQ=60)
                                           ATCAAGCCGCTGTATACCGAAGCCGATCTCGATAATCTGGAGGTGACAGGCACCCTTCCTGGTTTGCCGCCCTACGTTCGTGGCCCGCGTGCCA                                                    >  SRR4302537.6078371/1‑94 (MQ=60)
                                             CAAGCCGCTGTATACCGAAGCCGATCTCGATAATCTGGAGGTGACAGGCACCCTTCCTGGTTTGCCGCCCTACGTTCGTGGCCCGCGTGCCACT                                                  >  SRR4302537.3586771/1‑94 (MQ=60)
                                               AGCCGCTGTATACCGAAGCCGATCTCGATAATCTGGAGGTGACAGGCACCCTTCCTGGTTTGCCGCCCTACGTTCGTGGCCCGCGTGCCACTAT                                                <  SRR4302537.1913286/94‑1 (MQ=60)
                                               AGCCGCTGTATACCGAAGCCGATCTCGATAATCTGGAGGTGACAGGCACCCTTCCTGGTTTGCCGCCCTACGTTCGTGGCCCGCGTGCCACTAT                                                >  SRR4302537.432969/1‑94 (MQ=60)
                                                GCCGCTGTATACCGAAGCCGATCTCGATAATCTGGAGGTGACAGGCACCCTTCCTGGTTTGCCGCCCTACGTTCGTGGCCCGCGTGCCACTATG                                               <  SRR4302537.3513299/94‑1 (MQ=60)
                                                  CGCTGTATACCGAAGCCGATCTCGATAATCTGGAGGTGACAGGCACCCTTCCTGGTTTGCCGCCCTACGTTCGTGGCCCGCGTGCCACTATGTA                                             >  SRR4302537.5982994/1‑94 (MQ=60)
                                                  CGCTGTATACCGAAGCCGATCTCGATAATCTGGAGGTGACAGGCACCCTTCCTGGTTTGCCGCCCTACGTTCGTGGCCCGCGTGCCACTATGTA                                             >  SRR4302537.522135/1‑94 (MQ=60)
                                                   GCTGTATACCGAAGCCGATCTCGATAATCTGGAGGTGACAGGCACCCTTCCTGGTTTGCCGCCCTACGTTCGTGGCCCGCGTGCCACTATGTAT                                            <  SRR4302537.1025885/94‑1 (MQ=60)
                                                    CTGTATACCGAAGCCGATCTCGATAATCTGGAGGTGACAGGCACCCTTCCTGGTTTGCCGCCCTACGTTCGTGGCCCGCGTGCCACTATGTATA                                           <  SRR4302537.3530324/94‑1 (MQ=60)
                                                     TGTATACCGAAGCCGATCTCGATAATCTGGAGGTGACAGGCACCCTTCCTGGTTTGCCGCCCTACGTTCGTGGCCCGCGTGCCACTATGTATAC                                          >  SRR4302537.974815/1‑94 (MQ=60)
                                                     TGTATACCGAAGCCGATCTCGATAATCTGGAGGTGACAGGCACCCTTCCTGGTTTGCCGCCCTACGTTCGTGGCCCGCGTGCCACTATGTATAC                                          >  SRR4302537.1330517/1‑94 (MQ=60)
                                                     TGTATACCGAAGCCGATCTCGATAATCTGGAGGTGACAGGCACCCTTCCTGGTTTGCCGCCCTACGTTCGTGGCCCGCGTGCCACTATGTATAC                                          <  SRR4302537.6508772/94‑1 (MQ=60)
                                                     TGTATACCGAAGCCGATCTCGATAATCTGGAGGTGACAGGCACCCTTCCTGGTTTGCCGCCCTACGTTCGTGGCCCGCGTGCCACTATGTATAC                                          <  SRR4302537.249583/94‑1 (MQ=60)
                                                     TGTATACCGAAGCCGATCTCGATAATCTGGAGGTGACAGGCACCCTTCCTGGTTTGCCGCCCTACGTTCGTGGCCCGCGTGCCACTATGTATAC                                          >  SRR4302537.5048198/1‑94 (MQ=60)
                                                      GTATACCGAAGCCGATCTCGATAATCTGGAGGTGACAGGCACCCTTCCTGGTTTGCCGCCCTACGTTCGTGGCCCGCGTGCCACTATGTATACC                                         <  SRR4302537.1568534/94‑1 (MQ=60)
                                                      GTATACCGAAGCCGATCTCGATAATCTGGAGGTGACAGGCACCCTTCCTGGTTTGCCGCCCTACGTTCGTGGCCCGCGTGCCACTATGTATACC                                         >  SRR4302537.5223708/1‑94 (MQ=60)
                                                      GTATACCGAAGCCGATCTCGATAATCTGGAGGTGACAGGCACCCTTCCTGGTTTGCCGCCCTACGTTCGTGGCCCGCGTGCCACTATGTATACC                                         <  SRR4302537.2500568/94‑1 (MQ=60)
                                                      GTATACCGAAGCCGATCTCGATAATCTGGAGGTGACAGGCACCCTTCCTGGTTTGCCGCCCTACGTTCGTGGCCCGCGTGCCACTATGTATACC                                         >  SRR4302537.2784869/1‑94 (MQ=60)
                                                       TATACCGAAGCCGATCTCGATAATCTGGAGGTGACAGGCACCCTTCCTGGTTTGCCGCCCTACGTTCGTGGCCCGCGTGCCACTATGTATACCG                                        <  SRR4302537.4466974/94‑1 (MQ=60)
                                                         TACCGAAGCCGATCTCGATAATCTGGAGGTGACAGGCACCCTTCCTGGTTTGCCGCCCTACGTTCGTGGCCCGCGTGCCACTATGTATACCGCC                                      <  SRR4302537.881742/94‑1 (MQ=60)
                                                         TACCGAAGCCGATCTCGATAATCTGGAGGTGACAGGCACCCTTCCTGGTTTGCCGCCCTACGTTCGTGGCCCGCGTGCCACTATGTATACCGCC                                      <  SRR4302537.6462216/94‑1 (MQ=60)
                                                         TACCGAAGCCGATCTCGATAATCTGGAGGTGACAGGCACCCTTCCTGGTTTGCCGCCCTACGTTCGTGGCCCGCGTGCCACTATGTATACCGCC                                      <  SRR4302537.4844050/94‑1 (MQ=60)
                                                          ACCGAAGCCGATCTCGATAATCTGGAGGTGACAGGCACCCTTCCTGGTTTGCCGCCCTACGTTCGTGGCCCGCGTGCCACTATGTATACCGCCC                                     <  SRR4302537.2451163/94‑1 (MQ=60)
                                                            CGAAGCCGATCTCGATAATCTGGAGGTGCCAGGCACCCTTCCTGGTTTGCCGCCCTCCGTTCGTGGCCCGCGTGCCACTATGTATCCCGCCCAA                                   <  SRR4302537.2333424/94‑1 (MQ=60)
                                                            CGAAGCCGATCTCGATAATCTGGAGGTGACAGGCACCCTTCCTGGTTTGCCGCCCTACGTTCGTGGCCCGCGTGCCACTATGTATACCGCCCAA                                   >  SRR4302537.31588/1‑94 (MQ=60)
                                                             GAAGCCGATCTCGATAATCTGGAGGTGACAGGCGCCCTTCCTGGTTTGCCGCCCTACGTTCGTGGCCCGCGTGCCACTATGTATACCGCCCAAC                                  <  SRR4302537.2465540/94‑1 (MQ=60)
                                                             GAAGCCGATCTCGATAATCTGGAGGTGACAGGCACCCTTCCTGGTTTGCCGCCCTACGTTCGTGGCCCGCGTGCCACTATGTATACCGCCCAAC                                  >  SRR4302537.25220/1‑94 (MQ=60)
                                                              AAGCCGATCTCGATAATCTGGAGGTGACAGGCACCCTTCCTGGTTTGCCGCCCTACGTTCGTGGCCCGCGTGCCACTATGTATACCGCCCAACC                                 >  SRR4302537.1174909/1‑94 (MQ=60)
                                                              AAGCCGATCTCGATAATCTGGAGGTGACAGGCACCCTTCCTGGTTTGCCGCCCTACGTTCGTGGCCCGCGTGCCACTATGTATACCGCCCAACC                                 <  SRR4302537.2257208/94‑1 (MQ=60)
                                                              AAGCCGATCTCGATAATCTGGAGGTGACAGGCACCCTTCCTGGTTTGCCGCCCTACGTTCGTGGCCCGCGTGCCACTATGTATACCGCCCAACC                                 >  SRR4302537.5834928/1‑94 (MQ=60)
                                                               AGCCGATCTCGATAATCTGGAGGTGACAGGCACCCTTCCTGGTTTGCCGCCCTACGTTCGTGGCCCGCGTGCCACTATGTATACCGCCCAACCG                                >  SRR4302537.6136153/1‑94 (MQ=60)
                                                                 GCGATCTCGATAATCTGGAGGTGACAGGCACCCTTCCTGGTTTGCCGCCCTACGTTCGTGGCCCGCGTGCCACTATGTATACCGCCCAACCGTG                              <  SRR4302537.83675/94‑1 (MQ=60)
                                                                 CCGATCTCGATAATCTGGAGGTGACAGGCACCCTTCCTGGTTTGCCGCCCTACGTTCGTGGCCCGCGTGCCACTATGTATACCGCCCAACCGTG                              <  SRR4302537.3940747/94‑1 (MQ=60)
                                                                 CCGATCTCGATAATCTGGAGGTGACAGGCACCCTTCCTGGTTTGCCGCCCTACGTTCGTGGCCCGCGTGCCACTATGTATACCGCCCAACCGTG                              >  SRR4302537.4538163/1‑94 (MQ=60)
                                                                   GATCTCGATAATCTGGAGGTGACAGGCACCCTTCCTGGTTTGCCGCCCTACGTTCGTGGCCCGCGTGCCACTATGTATACCGCCCAACCGTGGA                            >  SRR4302537.6087717/1‑94 (MQ=60)
                                                                   CATCTCGATAATCTGGAGGTGACAGGCACCCTTCCTGGTTTGCCGCCCTACGTTCGTGGCCCGCGTGCCACTATGTATACCGCCCAACCGTGGA                            <  SRR4302537.5384166/94‑1 (MQ=60)
                                                                    ATCTCGATAATCTGGAGGTGACAGGCACCCTTCCTGGTTTGCCGCCCTACGTTCGTGGCCCGCGTGCCACTATGTATACCGCCCAACCGTGGAC                           <  SRR4302537.3520626/94‑1 (MQ=60)
                                                                      CTCGATAATCTGGAGGTGACAGGCACCCTTCCTGGTTTGCCGCCCTACGTTCGTGGCCCGCGTGCCACTATGTATACCGCCCAACCGTGGACCA                         >  SRR4302537.1646458/1‑94 (MQ=60)
                                                                      CTCGATAATCTGGAGGTGACAGGCACCCTTCCTGGTTTGCCGCCCTACGTTCGTGGCCCGCGTGCCACTATGTATACCGCCCAACCGTGGACCA                         >  SRR4302537.3484507/1‑94 (MQ=60)
                                                                      CTCGATAATCTGGAGGTGACAGGCACCCTTCCTGGTTTGCCGCCCTACGTTCGTGGCCCGCGTGCCACTATGTATACCGCCCAACCGTGGACCA                         >  SRR4302537.2375038/1‑94 (MQ=60)
                                                                        CGATAATCTGGAGGTGACAGGCACCCTTCCTGGTTTGCCGCCCTACGTTCGTGGCCCGCGTGCCACTATGTATACCGCCCAACCGTGGACCATC                       >  SRR4302537.906448/1‑94 (MQ=60)
                                                                          ATAATCTGGAGGTGACAGGCACCCTTCCTGGTTTGCCGCCCTACGTTCGTGGCCCGCGTGCCACTATGTATACCGCCCAACCGTGGACCATCCG                     <  SRR4302537.341890/94‑1 (MQ=60)
                                                                           TAATCTGGAGGTGACAGGCACCCTTCCTGGTTTGCCGCCCTACGTTCGTGGCCCGCGTGCCACTATGTATACCGCCCAACCGTGGACCATCCGT                    >  SRR4302537.5732565/1‑94 (MQ=60)
                                                                           TAATCTGGAGGTGACAGGCACCCTTCCTGGTTTGCCGCCCTACGTTCGTGGCCCGCGTGCCACTATGTATACCGCCCAACCGTGGACCATCCGT                    >  SRR4302537.1318068/1‑94 (MQ=60)
                                                                           TAATCTGGAGGTGACAGGCACCCTTCCTGGTTTGCCGCCCTACGTTCGTGGCCCGCGTGCCACTATGTATACCGCCCAACCGTGGACCATCCGC                    >  SRR4302537.2876892/1‑94 (MQ=60)
                                                                            AATCTGGAGGTGACAGGCACCCTTCCTGGTTTGCCGCCCTACGTTCGTGGCCCGCGTGCCACTATGTATACCGCCCAACCGTGGACCATCCGTC                   >  SRR4302537.3045125/1‑94 (MQ=60)
                                                                            AATCTGGAGGTGACAGGCACCCTTCCTGGTTTGCCGCCCTACGTTCGTGGCCCGCGTGCCACTATGTATACCGCCCAACCGTGGACCATCCGTC                   >  SRR4302537.4698979/1‑94 (MQ=60)
                                                                            AATCTGGAGGTGACAGGCACCCTTCCTGGTTTGCCGCCCTACGTTCGTGGCCCGCGTGCCACTATGTATACCGCCCAACCGTGGACCATCCGTC                   >  SRR4302537.1199409/1‑94 (MQ=60)
                                                                            AATCTGGAGGTGACAGGCACCCTTCCTGGTTTGCCGCCCTACGTTCGTGGCCCGCGTGCCACTATGTATACCGCCCAACCGTGGACCATCCGTC                   >  SRR4302537.6515356/1‑94 (MQ=60)
                                                                            AATCTGGAGGTGACAGGCACCCTTCCTGGTTTGCCGCCCTACGTTCGTGGCCCGCGTGCCACTATGTATACCGCCCAACCGTGGACCATCCGTC                   >  SRR4302537.4021737/1‑94 (MQ=60)
                                                                             ATCTGGAGGTGACAGGCACCCTTCCTGGTTTGCCGCCCTACGTTCGTGGCCCGCGTGCCACTATGTATACCGCCCAACCGTGGACCATCCGTCA                  <  SRR4302537.6475117/94‑1 (MQ=60)
                                                                             ATCTGGAGGTGACAGGCACCCTTCCTGGTTTGCCGCCCTACGTTCGTGGCCCGCGTGCCACTATGTATACCGCCCAACCGTGGACCATCCGTCA                  <  SRR4302537.4092365/94‑1 (MQ=60)
                                                                             ATCTGGAGGTGACAGGCACCCTTCCTGGTTTGCCGCCCTACGTTCGTGGCCCGCGTGCCACTATGTATACCGCCCAACCGTGGACCATCCGTCA                  >  SRR4302537.4127549/1‑94 (MQ=60)
                                                                             ATCTGGAGGTGACAGGCACCCTTCCTGGTTTGCCGCCCTACGTTCGTGGCCCGCGTGCCACTATGTATACCGCCCAACCGTGGACCATCCGTCA                  <  SRR4302537.4537758/94‑1 (MQ=60)
                                                                               CTGGAGGTGACAGGCACCCTTCCTGGTTTGCCGCCCTACGTTCGTGGCCCGCGTGCCACTATGTATACCGCCCAACCGTGGACCATCCGTCAGT                >  SRR4302537.1006456/1‑94 (MQ=60)
                                                                               CTGGAGGTGACAGGCACCCTTCCTGGTTTGCCGCCCTACGTTCGTGGCCCGCGTGCCACTATGTATACCGCCCAACCGCGGACCATCCGTCAGT                >  SRR4302537.2114822/1‑94 (MQ=60)
                                                                                 GGAGGTGACAGGCACCCTTCCTGGTTTGCCGCCCTACGTTCGTGGCCCGCGTGCCACTATGTATACCGCCCAACCGTGGACCATCCGTCAGTAT              <  SRR4302537.4730851/94‑1 (MQ=60)
                                                                                 GGAGGTGACAGGCACCCTTCCTGGTTTGCCGCCCTACGTTCGTGGCCCGCGTGCCACTATGTATACCGCCCAACCGTGGACCATCCGTCAGTAT              <  SRR4302537.5622365/94‑1 (MQ=60)
                                                                                  GAGGTGACAGGCACCCTTCCTGGTTTGCCGCCCTACGTTCGTGGCCCGCGTGCCACTATGTATACCGCCCAACCGTGGACCATCCGTCAGTATG             <  SRR4302537.1056388/94‑1 (MQ=60)
                                                                                    GGTGACAGGCCCCCTTCCTGGTTTGCCGCCCTACGTTCGTGGCCCGCGTGCCACTATGTATACCGCCCAACCGTGGACCATCCGTCAGTATGCT           <  SRR4302537.3574679/94‑1 (MQ=60)
                                                                                    GGTGACAGGCACCCTTCCTGGTTTGCCGCCCTACGTTCGTGGCCCGCGTGCCACTATGTATACCGCCCAACCGTGGACCATCCGTCAGTATGCT           <  SRR4302537.171232/94‑1 (MQ=60)
                                                                                     GTGACAGGCACCCTTCCTGGTTTGCCGCCCTACGTTCGTGGCCCGCGTGCCACTATGTATACCGCCCAACCGTGGACCATCCGTCAGTATGCTG          >  SRR4302537.679378/1‑94 (MQ=60)
                                                                                       GACAGGCACCCTTCCTGGTTTGCCGCCCTACGTTCGTGGCCCGCGTGCCACTATGTATACCGCCCAACCGTGGACCATCCGTCAGTATGCTGGT        >  SRR4302537.2444350/1‑94 (MQ=60)
                                                                                       GACAGGCACCCTTCCTGGTTTGCCGCCCTACGTTCGTGGCCCGCGTGCCACTATGTATACCGCCCAACCGTGGACCATCCGTCAGTATGCTGGT        <  SRR4302537.3668734/94‑1 (MQ=60)
                                                                                         CAGGCACCCTTCCTGGTTTGCCGCCCTACGTTCGTGGCCCGCGTGCCACTATGTATACCGCCCAACCGTGGACCATCCGTCAGTATGCTGGTTT      <  SRR4302537.1529467/94‑1 (MQ=60)
                                                                                         CAGGCACCCTTCCTGGTTTGCCGCCCTACGTTCGTGGCCCGCGTGCCACTATGTATACCGCCCAACCGTGGACCATCCGTCAGTATGCTGGTTT      >  SRR4302537.5441819/1‑94 (MQ=60)
                                                                                          AGGCACCCTTCCTGGTTTGCCGCCCTACGTTCGTGGCCCGCGTGCCACTATGTATACCGCCCAACCGTGGACCATCCGTCAGTATGCTGGTTTT     <  SRR4302537.3409923/94‑1 (MQ=60)
                                                                                          AGGCACCCTTCCTGGTTTGCCGCCCTACGTTCGTGGCCCGCGTGCCACTATGTATACCGCCCAACCGTGGACCATCCGTCAGTATGCTGGTTTT     >  SRR4302537.292650/1‑94 (MQ=60)
                                                                                           GGCACCCTTCCTGGTTTGCCGCCCTACGTTCGTGGCCCGCGTGCCACTATGTATACCGCCCAACCGTGGACCATCCGTCAGTATGCTGGTTTTT    <  SRR4302537.2280087/94‑1 (MQ=60)
                                                                                            GCACCCTTCCTGGTTTGCCGCCCTACGTTCGTGGCCCGCGTGCCACTATGTATACCGCCCAACCGTGGACCATCCGTCAGTATGCTGGTTTTTC   <  SRR4302537.713518/94‑1 (MQ=60)
                                                                                            GCACCCTTCCTGGTTTGCCGCCCTACGTTCGTGGCCCGCGTGCCACTATGTATACCGCCCAACCGTGGACCATCCGTCAGTATGCTGGTTTTTC   >  SRR4302537.6446210/1‑94 (MQ=60)
                                                                                             CACCCTTCCTGGTTTGCCGCCCTACGTTCGTGGCCCGCGTGCCACTATGTATACCGCCCAACCGTGGACCATCCGTCAGTATGCTGGTTTTTCA  <  SRR4302537.3156289/94‑1 (MQ=60)
                                                                                             |                                                                                             
AACTGTCGACTCGCTGGTTCATCAAACCGCGGAAGGGATCGCCATCAAGCCGCTGTATACCGAAGCCGATCTCGATAATCTGGAGGTGACAGGTACCCTTCCTGGTTTGCCGCCCTACGTTCGTGGCCCGCGTGCCACTATGTATACCGCCCAACCGTGGACCATCCGTCAGTATGCTGGTTTTTCA  >  NC_000913/3058929‑3059115

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 2 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑