Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I2 R1 882 254.4 12957588 98.0% 12698436 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,098,418 A→G G110G (GGT→GGC)  ansB ← periplasmic L‑asparaginase II

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,098,4180AG99.6% 879.4 / ‑5.1 256G110G (GGT→GGC) ansBperiplasmic L‑asparaginase II
Reads supporting (aligned to +/- strand):  ref base A (0/0);  major base G (122/133);  minor base T (1/0);  total (123/133)
Fisher's exact test for biased strand distribution p-value = 4.80e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.31e-01

Only 100 of 264 total aligned reads displayed.

GGACGGACGCATTGCGCCGACCATCACCACCGGTTTGTCGCATTTCACCGTCAGGTCGAGGAAGTAAGCAGTTTCTTCCATCGTGTCGGTACCGTGGGTAATGACGAAGCCGTCGGTCTTATCGCAGTCGGTGTTAATTTTTTTCGCCAGTGTCAGCCAGACATTATCGTTCATGTCCTG  >  NC_000913/3098328‑3098507
                                                                                          |                                                                                         
 gacggacgCATTGCGCCGACCATCACCACCGGTTTGTCGCATTTCACCGTCAGGTCGAGGAAGTAAGCAGTTTCTTCCATCGTGTCGGTGCCg                                                                                        >  2:5920682/1‑93 (MQ=255)
  acggacgCATTGCGCCGACCATCACCACCGGTTTGTCGCATTTCACCGTCGGGTCGAGGAAGTAAGCAGTTTCTTCCATCGTGTCGGTGCCGt                                                                                       <  1:3455171/93‑1 (MQ=255)
  acggacgCATTGCGCCGACAATCACCACCGGTTTGTCGCATTTCACCGTCAGGTCGAGGAAGTAAGCAGTTTCTTCCATCGTGTCGGTGCCGt                                                                                       <  2:4199934/93‑1 (MQ=255)
      acgCATTGCGCCGACCATCACCACCGGTTTGTCGCATTTCACCGTCAGGTCGAGGAAGTAAGCAGTTTCTTCCATCGTGTCGGTGCCGTGGGt                                                                                   >  1:3683189/1‑93 (MQ=255)
        gCATTGCGCCGACCATCACCACCGGTTTGTCGCATTTCACCGTCAGGTCGAGGAAGTAAGCAGTTTCTTCCATCGTGTCGGTGCCGTGGGTaa                                                                                 >  1:1494469/1‑93 (MQ=255)
        gCATTGCGCCGACCATCACCACCGGTTTGTCGCATTTCACCGTCAGGTCGAGGAAGTAAGCAGTTTCTTCCATCGTGTCGGTGCCGTGGGTaa                                                                                 >  2:3816554/1‑93 (MQ=255)
         cATTGCGCCGACCATCACCACCGGTTTGTCGCATTTCACCGTCAGGTCGAGGAAGTAAGCAGTTTCTTCCATCGTGTCGGTGCCGTGGGTAAt                                                                                <  2:5006579/93‑1 (MQ=255)
           ttGCGCCGACCATCACCACCGGTTTGTCGCATTTCACCGTCAGGTCGAGGAAGTAAGCAGTTTCTTCCATCGTGTCGGTGCCGTGGGTAATGa                                                                              <  1:6051226/93‑1 (MQ=255)
               gcCGACCATCACCACCGGTTTGTCGCATTTCACCGTCAGGTCGAGGAAGTAAGCAGTTTCTTCCATCGTGTCGGTGCCGTGGGTAATGACGaa                                                                          >  2:6011239/1‑93 (MQ=255)
               gcCGACCATCACCACCGGTTTGTCGCATTTCACCGTCAGGTCGAGGAAGTAAGCAGTTTCTTCCATCGTGTCGGTGCCGTGGGTAATGACGaa                                                                          <  2:2387785/93‑1 (MQ=255)
               cccGACCATCACCACCGGTTTGTCGCATTTCACCGTCAGGTCGAGGAAGTAAGCAGTTTCTTCCATCGTGTCGGTGCCGTGGGTAATGACGaa                                                                          <  2:2760424/92‑1 (MQ=255)
                ccGACCATCACCACCGGTTTGTCTCATTTCACCGTCAGGTCGAGGAAGTAAGCAGTTTCTTCCATCGTGTCGGTGCCGTGGGTAATGACGAAg                                                                         <  1:1236591/93‑1 (MQ=255)
                  gACCATCACCACCGGTTTGTCGCATTTCACCGTCAGGTCGAGGAAGTAAGCAGTTTCTTCCATCGTGTCGGTGCCGTGGGTAATGACGAAGcc                                                                       >  2:5276604/1‑93 (MQ=255)
                  gACCATCACCACCGGTTTGTCGCATTTCACCGTCAGGTCGAGGAAGTAAGCAGTTTCTTCCATCGTGTCGGTGCCGTGGGTAATGACGAAGcc                                                                       >  1:4442724/1‑93 (MQ=255)
                   aCCATCACCACCGGTTTGTCGCATTTCACCGTCAGGTCGAGGAAGTAAGCAGTTTCTTCCATCGTGTCGGCGCCGTGGGCAATGACGAAGCCg                                                                      >  2:3490894/1‑93 (MQ=255)
                    ccATCACCACCGGTTTGTCGCATTTCACCGTCAGGTCGAGGAAGTAAGCAGTTTCTTCCATCGTGTCGGTGCCGTGGGTAATGACGAAGCCGt                                                                     <  2:3884047/93‑1 (MQ=255)
                     cATCACCACCGGTTTGTCGCATTTCACCGTCAGGTCGAGGAAGTAAGCAGTTTCTTCCATCGTGTCGGTGCCGTGGGTAATGACGAAGCCGTc                                                                    >  1:2149389/1‑93 (MQ=255)
                     cATCACCACCGGTTTGTCGCATTTCACCGTCAGGTCGAGGAAGTAAGCAGTTTCTTCCATCGTGTCGGTGCCGTGGGTAATGACGAAGCCGTc                                                                    >  1:5162636/1‑93 (MQ=255)
                     cATCACCACCGGTTTGTCGCATTTCACCGTCAGGTCGAGGAAGTAAGCAGTTTCTTCCATCGTGTCGGTGCCGTGGGTAATGACGAAGCCGTc                                                                    >  2:568537/1‑93 (MQ=255)
                     cATCACCACCGGTTTGTCGCATTTCACCGTCAGGTCGAGGAAGTAAGCAGTTTCTTCCATCGTGTCGGTGCCGTGGGTAATGACGAAGCCGTc                                                                    <  1:4518669/93‑1 (MQ=255)
                     cATCACCACCGGTTTGTCGCATTTCACCGTCAGGTCGAGGAAGTAAGCAGTTTCTTCCATCGTGTCGGTGCCGTGGGTAATGACGAAGCCGTc                                                                    >  2:2161393/1‑93 (MQ=255)
                      aTCACCACCGGTTTGTCGCATTTCACCGTCAGGTCGAGGAAGTAAGCAGTTTCTTCCATCGTGTCGGTGCCGTGGGTAATGACGAAGCCGTCg                                                                   >  2:2524767/1‑93 (MQ=255)
                      aTCACCACCGGTTTGTCGCATTTCACCGTCAGGTCGAGGAAGTAAGCAGTTTCTTCCATCGTGTCGGTGCCGTGGGTAATGACGAAGCCGTCg                                                                   >  1:5306997/1‑93 (MQ=255)
                       tCACCACCGGTTTGTCGCATTTCACCGTCAGGTCGAGGAAGTAAGCAGTTTCTTCCATCGTGTCGGTGCCGTGGGTAATGACGAAGCCGTCgg                                                                  >  1:3840888/1‑93 (MQ=255)
                       tCACCACCGGTTTGTCGCATTTCACCGTCAGGTCGAGGAAGTAAGCAGTTTCTTCCATCGTGTCGGTGCCGTGGGTAATGACGAAGCCGTCgg                                                                  >  2:4345700/1‑93 (MQ=255)
                         cccaccGGTTTGTCGCATTTCACCGTCAGGTCGAGGAAGTAAGCAGTTTCTTCCATCGTGTCGGTGCCGTGGGTAATGACGAAGCCGTCGGTc                                                                <  2:293269/92‑1 (MQ=255)
                         accaccGGTTTGTCGCATTTCACCGTCAGGTCGAGGAAGTAAGCAGTTTCTTCCATCGTGTCGGTGCCGTGGGTAATGACGAAGCCGTCGGTc                                                                <  2:2049302/93‑1 (MQ=255)
                          ccaccGGTTTGTCGCATTTCACCGTCAGGTCGAGGAAGTAAGCAGTTTCTTCCATCGTGTCGGTGCCGTGGGTAATGACGAAGCCGTCGGTCt                                                               >  1:578078/1‑93 (MQ=255)
                          ccaccGGTTTGTCGCATTTCACCGTCAGGTCGAGGAAGTAAGCAGTTTCTTCCATCGTGTCGGTGCCGTGGGTAATGACGAAGCCGTCGGTCt                                                               <  1:1000628/93‑1 (MQ=255)
                          ccaccGGTTTGTCGCATTTCACCGTCAGGTCGAGGAAGTAAGCAGTTTCTTCCATCGTGTCGGTGCCGTGGGTAATGACGAAGCCGTCGGGCt                                                               >  1:691776/1‑93 (MQ=255)
                           caccGTTTTGTCGCATTTCACCGTCAGGTCGAGGAAGTAAGCAGTTTCTTCCATCGTGTCGGTGCCGTGGGTAATGACGAAGCCGTCGGTCtt                                                              <  2:4884870/93‑1 (MQ=255)
                           caccGGTTTGTCGCATTTCACCGTCAGGTCGAGGAAGTAAGCAGTTTCTTCCATCGTGTCGGTGCCGTGGGTAATGACGAAGCCGTCGGTCtt                                                              >  2:1989729/1‑93 (MQ=255)
                           caccGGTTTGTCGCATTTCACCGTCAGGTCGAGGAAGTAAGCAGTTTCTTCCATCGTGTCGGTGCCGTGGGTAATGACGAAGCCGTCGGTCtt                                                              >  1:97369/1‑93 (MQ=255)
                           caccGGTTTGTCGCATTTCACCGTCAGGTCGAGGAAGTAAGCAGTTTCTTCCATCGTGTCGGTGCCGTGGGTAATGACGAAGCCGTCGGTCtt                                                              <  2:2863391/93‑1 (MQ=255)
                            accGGTTTGTCGCATTTCACCGTCAGGTCGAGGAAGTAAGCAGTTTCTTCCATCGTGTCGGTGCCGTGGGTAATGACGAAGCCGTCGGTCTTa                                                             <  1:3752934/93‑1 (MQ=255)
                             ccGGTTTGTCGCATTTCACCGTCAGGTCGAGGAAGTAAGCAGTTTCTTCCATCGTGTCGGTGCCGTGGGTAATGACGAAGCCGTCGGTCTTAt                                                            >  2:4654898/1‑93 (MQ=255)
                                gTTTGTCGCATTTCACCGTCAGGTCGAGGAAGTAAGCAGTTTCTTCCATCGTGTCGGTGCCGTGGGTAATGACGAAGCCGTCGGTCTTATCGc                                                         >  1:2213564/1‑93 (MQ=255)
                                gTTTGTCGCATTTCACCGTCAGGTCGAGGAAGTAAGCAGTTTCTTCCATCGTGTCGGTGCCGTGGGTAATGACGAAGCCGTCGGTCTTATCGc                                                         >  1:4319743/1‑93 (MQ=255)
                                  ttGTCGCATTTCACCGTCAGGTCGAGGAAGTAAGCAGTTTCTTCCATCGTGTCGGTGCCGTGGGTAATGACGAAGCCGTCGGTCTTATCGCAg                                                       >  1:2312069/1‑93 (MQ=255)
                                  ttGTCGCATTTCACCGTCAGGTCGAGGAAGTAAGCAGTTTCTTCCATCGTGTCGGTGCCGTGGGTAATGACGAAGCCGTCGGTCTTATCGCAg                                                       <  1:2410635/93‑1 (MQ=255)
                                    gTCGCATTTCACCGTCAGGTCGATGAAGTAAGCAGTTTCTTCCATCGTGTCGGTGCCGTGGGTAATGACGAAGCCGTCGGTCTTATCGCAGtg                                                     >  1:2073652/1‑92 (MQ=255)
                                    gTCGCATTTCACCGTCAGGTCGAGGAAGTAAGCAGTTTCTTCCATCGTGTCGGTGCCGTGGGTAATGACGAAGCCGTCGGTCTTATCGCAGTc                                                     <  1:780306/93‑1 (MQ=255)
                                     tCGCATTTCACCGTCAGGTCGAGGAAGTAAGCAGTTTCTTCCATCGTGTCGGTGCCGTGGGTAATGACGAAGCCGTCGGTCTTATCGCAGTCg                                                    >  1:4810199/1‑93 (MQ=255)
                                      cGCATTTCACCGTCAGGTCGAGGAAGTAAGCAGTTTCTTCCATCGTGTCGGTGCCGTGGGTAATGACGAAGCCGTCGGTCTTATCGCAGTCgg                                                   <  1:596659/93‑1 (MQ=255)
                                         aTTTCACCGTCAGGTCGAGGAAGTAAGCAGTTTCTTCCATCGTGTCGGTGCCGTGGGTAATGACGAAGCCGTCGGTCTTATCTCAGTCGgtgt                                                >  1:1531767/1‑93 (MQ=255)
                                         aTTTCACCGTCAGGTCGAGGAAGTAAGCAGTTTCTTCCATCGTGTCGGTGCCGTGGGTAATGACGAAGCCGTCGGTCTTATCGCAGTCGgtgt                                                >  2:5553335/1‑93 (MQ=255)
                                           ttCACCGTCAGGTCGAGGAAGTAAGCAGTTTCTTCCATCGTGTCGGTGCCGTGGGTAATGACGAAGCCGTCGGTCTTATCGCAGTCGGTGTTa                                              <  1:1857490/93‑1 (MQ=255)
                                            tCACCGTCAGGTCGAGGAAGTAAGCAGTTTCTTCCATCGTGTCGGTGCCGCGGGTAATGACGAAGGCGTCCGGCTTATCGCAGTCGGTGTTat                                             >  2:5487931/1‑92 (MQ=255)
                                             cACCGTCAGGTCGAGGAAGTAAGCAGTTTCTTCCATCGTGTCGGTGCCGTGGGTAATGACGAAGCCGTCGGTCTTATCGCAGTCGGTGTTAAt                                            <  2:3302006/93‑1 (MQ=255)
                                             cACCGTCAGGTCGAGGAAGTAAGCAGTTTCTTCCATCGTGTCGGTGCCGTGGGTAATGACGAAGCCGTCGGTCTTATCGCAGTCGGTGTTAAt                                            <  2:6360418/93‑1 (MQ=255)
                                               ccGTCAGGTCGAGGAAGTAAGCAGTTTCTTCCATCGTGTCGGTGCCGTGGGTAATGACGAAGCCGTCGGTCTTATCGCAGTCGGTGTTAAttt                                          <  1:5048049/93‑1 (MQ=255)
                                                cGTCAGGTCGAGGAAGTAAGCAGTTTCTTCCATCGTGTCGGTGCCGTGGGTAATGACGAAGCCGTCGGTCTTATCGCAGTCGGTGTTAAtttt                                         >  1:5529576/1‑93 (MQ=255)
                                                 gTCAGGTCGAGGAAGTAAGCAGTTTCTTCCATCGTGTCGGTGCCGTGGGTAATGACGAAGCCGTCGGTCTTATCGCAGTCGGTGTTAAttttt                                        >  1:1194440/1‑93 (MQ=255)
                                                  tCAGGTCGAGGAAGTAAGCAGTTTCTTCCATCGTGTCGGTGCCGTGGGTAATGACGAAGCCGTCGGTCTTATCGCAGTCGGTGTTAAtttttt                                       <  1:1384820/93‑1 (MQ=255)
                                                   cAGGTCGAGGAAGTAAGCAGTTTCTTCCATCGTGTCGGTGCCGTGGGTAATGACGAAGCCGTCGGTCTTATCGCAGTCGGTGTTAAttttttt                                      <  1:2824250/93‑1 (MQ=255)
                                                   cAGGTCGAGGAAGTAAGCAGTTTCTTCCATCGTGTCGGTGCCGTGGGTAATGACGAAGCCGTCGGTCTTATCGCAGTCGGTGTTAAttttttt                                      >  2:3802415/1‑93 (MQ=255)
                                                    aGGTCGAGGAAGTAAGCAGTTTCTTCCATCGTGTCGGTGCCGTGGGTAATGACGAAGCCGTCGGTCTTATCGCAGTCGGTGTTAATTTTTTTc                                     <  1:5656691/93‑1 (MQ=255)
                                                    aGGTCGAGGAAGTAAGCAGTTTCTTCCATCGTGTCGGTGCCGTGGGTAATGACGAAGCCGTCGGTCTTATCGCAGTCGGTGTTAATTTTTTTc                                     >  2:396567/1‑93 (MQ=255)
                                                    aGGTCGAGGAAGTAAGCAGTTTCTTCCATCGTGTCGGTGCCGTGGGTAATGACGAAGCCGTCGGTCTTATCGCAGTCGGTGTTAATTTTTTTc                                     <  1:4214780/93‑1 (MQ=255)
                                                     ggTCGAGGAAGTAAGCAGTTTCTTCCATCGTGTCGGTGCCGTGGGTAATGACGAAGCCGTCGGTCTTATCGCAGTCGGTGTTAATTTTTTTCg                                    >  1:4022277/1‑93 (MQ=255)
                                                      gTCGAGGAAGTAAGCAGTTTCTTCCATCGTGTCGGTGCCGTGGGTAATGACGAAGCCGTCGGTCTTATCGCAGTCGGTGTTAATTTTTTTCGc                                   <  2:3179297/93‑1 (MQ=255)
                                                          aGGAAGTAAGCAGTTTCTTCCATCGTGTCGGTGCCGTGGGTAATGACGAAGCCGTCGGTCTTATCGCAGTCGGTGTTAATTTTTTTCGCCAgt                               <  1:6148726/93‑1 (MQ=255)
                                                          aGGAAGTAAGCAGTTTCTTCCATCGTGTCGGTGCCGTGGGTAATGACGAAGCCGTCGGTCTTATCGCAGTCGGTGTTAATTTTTTTCGCCAgt                               >  1:2175477/1‑93 (MQ=255)
                                                           ggAAGTAAGCAGTTTCTTCCATCGTGTCGGTGCCGTGGGTAATGACGAAGCCGTCGGTCTTATCGCAGTCGGTGTTAATTTTTTTCGCCAgtg                              >  1:3980898/1‑93 (MQ=255)
                                                               gTAAGCAGTTTCTTCCATCGTGTCGGTGCCGTGGGTAATGACGAAGCCGTCGGTCTTATCGCAGTCGGTGTTAATTTTTTTCGCCAGTGTCAg                          >  2:4532878/1‑93 (MQ=255)
                                                               gTAAGCAGTTTCTTCCATCGTGTCGGTGCCGTGGGTAATGACGAAGCCGTCGGTCTTATCGCAGTCGGTGTTAATTTTTTTCGCCAGTGTCAg                          <  2:1618984/93‑1 (MQ=255)
                                                               gTAAGCAGTTTCTTCCATCGTGTCGGTGCCGTGGGTAATGACGAAGCCGTCGGTCTTATCGCAGTCGGTGTTAATTTTTTTCGCCAGTGTCAg                          <  1:2626759/93‑1 (MQ=255)
                                                                tAAGCAGTTTCTTCCATCGTGTCGGTGCCGTGGGTAATGACGAAGCCGTCGGTCTTATCGCAGTCGGTGTTAATTTTTTTCGCCAGTGTCAGc                         <  2:786824/93‑1 (MQ=255)
                                                                 aaGCAGTTTCTTCCATCGTGTCGGTGCCGTGGGTAATGACGAAGCCGTCGGTCTTATCGCAGTCGGTGTTAATTTTTTTCGCCAGTGTCAGcc                        >  2:4918777/1‑93 (MQ=255)
                                                                   gCAGTTTCTTCCATCGTGTCGGTGCCGTGGGTAATGACGAAGCCGTCGGTCTTATCGCAGTCGGTGTTAATTTTTTTCGCCAGTGTCAGCCAg                      <  2:755878/93‑1 (MQ=255)
                                                                     aGTTTCTTCCATCGTGTCGGTGCCGTGGGTAATGACGAAGCCGTCGGTCTTATCGCAGTCGGTGTTAATTTTTTTCGCCAGTGTCAGCCAGAc                    <  2:5099927/93‑1 (MQ=255)
                                                                       tttCTTCCATCGTGTCGGTGCCGTGGGTAATGACGAAGCCGTCGGTCTTATCGCAGTCGGTGTTAATTTTTTTCGCCAGTGTCAGCCAGACAt                  <  2:1330312/93‑1 (MQ=255)
                                                                       tttCTTCCATCGTGTCGGTGCCGTGGGTAATGACGAAGCCGTCGGTCTTATCGCAGTCGGCGTTAATTTTTTTCGCCAGTGTCAGCCAGACAt                  >  1:1072487/1‑93 (MQ=255)
                                                                        ttcttcCATCGTGTCGGTGCCGTGGGTAATGACGAAGCCGTCGGTCTTATCGCAGTCGGTGTTAATTTTTTTCGCCAGTGTCAGCCAGACAtt                 >  2:5244635/1‑93 (MQ=255)
                                                                         tcttcCATCGTGTCGGTGCCGTGGGTAATGACGAAGCCGTCGGTCTTATCGCAGTCGGTGTTAATTTTTTTCGCCAGTGTCAGCCAGACATTa                <  2:1418161/93‑1 (MQ=255)
                                                                          cttcCATCGTGTCGGTGCCGTGGGTAATGACGAAGCCGTCGGTCTTATCGCAGTCGGTGTTAATTTTTTTCGCCAGTGTCAGCCAGACATTAt               >  1:2949246/1‑93 (MQ=255)
                                                                           ttcCATCGTGTCGGTTCCGTGGGTAATGACGAAGCCGTCGGTCTTATCGCAGTCGGAGTTAATTTTTTTCGCCAGTGTCAGCCAGACATTATc              >  2:3120168/1‑93 (MQ=255)
                                                                           ttcCATCGTGTCGGTGCCGTGGGTAATGACGAAGCCGTCGGTCTTATCGCAGTCGGTGTTAATTTTTTTCGCCAGTGTCAGCCAGACATTATc              >  1:1646242/1‑93 (MQ=255)
                                                                           ttcCATCGTGTCGGTGCCGTGGGTAATGACGAAGCCGTCGGTCTTATCGCAGTCGGTGTTAATTTTTTTCGCCAGTGTCAGCCAGACATTATc              >  2:3617312/1‑93 (MQ=255)
                                                                            tcCATCGTGTCGGTGCCGTGGGTAATGACGAAGCCGTCGGTCTTATCGCAGTCGGTGTTAATTTTTTTCGCCAGTGTCAGCCAGACATTATCg             <  1:3531969/93‑1 (MQ=255)
                                                                            tcCATCGTGTCGGTGCCGGGGGTAATGACGAAGCCGTCGGTCTTATCGCAGTCGGGGTTAATTTTTTTCGCCAGTGTCAGCCAGACATTATCg             >  2:4712137/1‑93 (MQ=255)
                                                                             ccATCGTGTCGGTGCCGTGGGTAATGACGAAGCCGTCGGTCTTATCGCAGTCGGTGTTAATTTTTTTCGCCAGTGTCAGCCAGACATTATCGt            <  1:3215032/93‑1 (MQ=255)
                                                                              cATCGTGTCGGTGCCGTGGGTAATGACGAAGCCGTCGGTCTTATCGCAGTCGGTGTTAATTTTTTTCGCCAGTGTCAGCCAGACATTATCGtt           <  2:374824/93‑1 (MQ=255)
                                                                              cATCGTGTCGGTGCCGTGGGTAATGACGAAGCCGTCGGTCTTATCGCAGTCGGTGTCAATTTTTTTCGCCACCGTCAGCCACACACTATCGtt           >  2:5852202/1‑93 (MQ=255)
                                                                              cATCGTGTCGGTGCCGTGGGTAATGACGAAGCCGTCGGGCTTATCGCAGTCGGCGTTAATTTGTTTCGCCAGTGTCAGCCAGACATTATCGtt           >  1:4680361/1‑93 (MQ=255)
                                                                              cATCGTGTCGCTGCGGTATGTAATGACGAAGCCGTCGGTCTTATCGCAGTCGGTGTTAATTTTTTTCGCCAGTGTCAGCCAGACATTATCGtt           <  2:6085864/93‑1 (MQ=255)
                                                                               aTCGTGTCGGTGCCGTGGGTAATGACGAAGCCGTCGGTCTTATCGCAGTCGGTGTTAATTTTTTTCGCCAGTGTCAGCCAGACATTATCGTTc          >  1:6230262/1‑93 (MQ=255)
                                                                               aTCGTGTCGGTGCCGTGGGTAATGACGAAGCCGTCGGTCTTATCGCAGTCGGTGTTAATTTTTTTCGCCAGTGTCAGCCAGACATTATCGTTc          <  1:4654898/93‑1 (MQ=255)
                                                                                tCGTGTCGGTGCCGTGGGTAATGACGAAGCCGTCGGTCTTATCGCAGTCGGTGTTAATTTTTTTCGCCAGTGTCAGCCAGACATTATCGTTCa         >  1:877403/1‑93 (MQ=255)
                                                                                tCGTGTCGGTGCCGTGGGTAATGACGAAGCCGTCGGTCTTATCGCAGTCGGTGTTAATTTTTTTCGCCAGTGTCAGCCAGACATTATCGTTCa         <  1:2619574/93‑1 (MQ=255)
                                                                                  gtgtCGGTGCCGTGGGTAATGACGAAGCCGTCGGTCTTATCGCAGTCGGTGTTAATTTTTTTCGCCAGTGTCAGCCAGACATTATCGTTCATg       >  1:6436286/1‑93 (MQ=255)
                                                                                  gtgtCGGTGCCGTGGGTAATGACGAAGCCGTCGGTCTTATCGCAGTCGGTGTTAATTTTTTTCGCCAGTGTCAGCCAGACATTATCGTTCATg       >  1:528373/1‑93 (MQ=255)
                                                                                  gtgtCGGTGCCGTGGGTAATGACGAAGCCGTCGGTCTTATCGCAGTCGGTGTTAATTTTTTTCGCCAGTGTCAGCCAGACATTATCGTTCATg       >  2:6306740/1‑93 (MQ=255)
                                                                                   tgtCGGGGCCGCGGGGAATGACGAAGCCGTCGGGCTTATCGCAGTCGGTGTTAATTTTTTTCGCCAGTGGCAGCCAGACATTATCGGTCATGt      >  2:1140788/1‑93 (MQ=255)
                                                                                    gtCGGTGCCGTGGGTAATGACGAAGCCGTCGGTCTTATCGCAGTCGGTGTTAATTTTTTTCGCCAGTGTCAGCCAGACATTATCGTTCATGTc     <  1:1928232/93‑1 (MQ=255)
                                                                                      cGGTGCCGTGGGTAATGACGAAGCCGTCGGTCTTATCGCAGTCGGTGTTAATTTTTTTCGCCAGTGTCAGCCAGACATTATCGTTCATGTCCt   >  2:1079098/1‑93 (MQ=255)
                                                                                      cGGTGCCGTGGGTAATGACGAAGCCGTCGGTCTTATCGCAGTCGGTGTTAATTTTTTTCGCCAGTGTCAGCCAGACATTATCGTTCATGTCCt   <  1:3002149/93‑1 (MQ=255)
                                                                                       ggTGCCGTGGGTAATGACGAAGCCGTCGGTCTTATCGCAGTCGGTGTTAATTTTTTTCGCCAGTGTCAGCCAGACATTATCGTTCATGTCCTg  <  2:539551/93‑1 (MQ=255)
                                                                                       ggTGCCGTGGGTAATGACGAAGCCGTCGGTCTTATCGCAGTCGGTGTTAATTTTTTTCGCCAGTGTCAGCCAGACATTATCGTTCATGTCCTg  <  2:1882785/93‑1 (MQ=255)
                                                                                       ggTGCCGTGGGTAATGACGAAGCCGTCGGTCTTATCGCAGTCGGTGTTAATTTTTTTCGCCAGTGTCAGCCAGACATTATCGTTCATGTCCTg  >  2:907733/1‑93 (MQ=255)
                                                                                          |                                                                                         
GGACGGACGCATTGCGCCGACCATCACCACCGGTTTGTCGCATTTCACCGTCAGGTCGAGGAAGTAAGCAGTTTCTTCCATCGTGTCGGTACCGTGGGTAATGACGAAGCCGTCGGTCTTATCGCAGTCGGTGTTAATTTTTTTCGCCAGTGTCAGCCAGACATTATCGTTCATGTCCTG  >  NC_000913/3098328‑3098507

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 19 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
TGGACGGACGCATTGCGCCGACCATCACCACCGGTTTGTCGCATTTCACCGTCAGGTCGAGGAAGTAAGCAGTTTCTTCCATCGTGTCGGTACCGTGGGTAATGACGAAGCCGTCGGTCTTATCGCAGTCGGTGTTAATTTTTTTCGCCAGTGTCAGCCAGACATTATCGTTCATGTCCTGGGAG  >  NC_000913/3098327‑3098511
                                                                                           |                                                                                             
TGGACGGACGCATTGCGCCGACCATCACCACCGGTTTGTCGCATTTCACCGTCAGGTCGAGGAAGTAAGCAGTTTCTTCCATCGTGTCGGTGCC                                                                                             >  SRR4302537.1458852/1‑94 (MQ=60)
 GGACGGACGCATTGCGCCGACCATCACCACCGGTTTGTCGCATTTCACCGTCAGGTCGAGGAAGTAAGCAGTTTCTTCCATCGTGTCGGTGCCG                                                                                            >  SRR4302537.945705/1‑94 (MQ=60)
   ACGGACGCATTGCGCCGACCATCACCACCGGTTTGTCGCATTTCACCGTCGGGTCGAGGAAGTAAGCAGTTTCTTCCATCGTGTCGGTGCCGTG                                                                                          <  SRR4302537.3479609/94‑1 (MQ=60)
    CGGACGCATTGCGCCGACCATCACCACCGGTTTGTCGCATTTCACCGTCAGGTCGAGGAAGTAAGCAGTTTCTTCCATCGTGTCGGTGCCGTGG                                                                                         <  SRR4302537.4254141/94‑1 (MQ=60)
    CGGACGCATTGCGCCGACCATCACCACCGGTTTGTCGCATTTCACCGTCAGGTCGAGGAAGTAAGCAGTTTCTTCCATCGTGTCGGTGCCGTGG                                                                                         <  SRR4302537.723093/94‑1 (MQ=60)
     GGACGCATTGCGCCGACCATCACCACCGGTTTGTCGCATTTCACCGTCAGGTCGAGGAAGTAAGCAGTTTCTTCCATCGTGTCGGTGCCGTGGG                                                                                        >  SRR4302537.5518624/1‑94 (MQ=60)
      GACGCATTGCGCCGACCATCACCACCGGTTTGTCGCATTTCACCGTCAGGTCGAGGAAGTAAGCAGTTTCTTCCATCGTGTCGGTGCCGTGGGT                                                                                       >  SRR4302537.3709091/1‑94 (MQ=60)
      GACGCATTGCGCCGACCATCACCACCGGTTTGTCGCATTTCACCGTCAGGTCGAGGAAGTAAGCAGTTTCTTCCATCGTGTCGGTGCCGTGGGT                                                                                       <  SRR4302537.4097731/94‑1 (MQ=60)
       ACGCATTGCGCCGACCATCACCACCGGTTTGTCGCATTTCACCGTCAGGTCGAGGAAGTAAGCAGTTTCTTCCATCGTGTCGGTGCCGTGGGTA                                                                                      >  SRR4302537.5283050/1‑94 (MQ=60)
        CGCATTGCGCCGACCATCACCACCGGTTTGTCGCATTTCACCGTCAGGTCGAGGAAGTAAGCAGTTTCTTCCATCGTGTCGGTGCCGTGGGTAA                                                                                     >  SRR4302537.1504882/1‑94 (MQ=60)
           ATTGCGCCGACCATCACCACCGGTTTGTCGCATTTCACCGTCAGGTCGAGGAAGTAAGCAGTTTCTTCCATCGTGTCGGTGCCGTGGGTAATGA                                                                                  >  SRR4302537.675642/1‑94 (MQ=60)
            TTGCGCCGACCATCACCACCGGTTTGTCGCATTTCACCGTCAGGTCGAGGAAGTAAGCAGTTTCTTCCATCGTGTCGGTGCCGTGGGTAATGAC                                                                                 <  SRR4302537.6092792/94‑1 (MQ=60)
            TTGCGCCGACCATCACCACCGGTTTGTCGCATTTCACCGTCAGGTCGAGGAAGTAAGCAGTTTCTTCCATCGTGTCGGTGCCGTGGGTAATGAC                                                                                 <  SRR4302537.1373432/94‑1 (MQ=60)
               CGCCGACCATCACCACCGGTTTGTCGCATTTCACCGTCAGGTCGAGGAAGTAAGCAGTTTCTTCCATCGTGTCGGTGCCGTGGGTAATGACGAA                                                                              <  SRR4302537.3873972/94‑1 (MQ=60)
                 CCGACCATCACCACCGGTTTGTCTCATTTCACCGTCAGGTCGAGGAAGTAAGCAGTTTCTTCCATCGTGTCGGTGCCGTGGGTAATGACGAAGC                                                                            <  SRR4302537.1245195/94‑1 (MQ=60)
                  CGACCTTCACCACCGGTTTGTCGCGTTTCACCGTCAGGTCGAGGAAGTAAGCAGTTTCTTCCATCGTGTCGGTGCCGTGGGTAATGACGAAGCC                                                                           <  SRR4302537.2103590/94‑1 (MQ=60)
                  CGACCATCACCACCGGTTTGTCGCATTTCACCGTCAGGTCGAGGAAGTAAGCAGTTTCTTCCATCGTGTCGGTGCCGTGGGTAATGACGAAGCC                                                                           >  SRR4302537.4473409/1‑94 (MQ=60)
                   GACCATCACCACCGGTTTGTCGCATTTCACCGTCAGGTCGAGGAAGTAAGCAGTTTCTTCCATCGTGTCGGTGCCGTGGGTAATGACGAAGCCG                                                                          >  SRR4302537.5609438/1‑94 (MQ=60)
                   GACCATCACCACCGGTTTGTCGCATTTCACCGTCAGGTCGAGGAAGTAAGCAGTTTCTTCCATCGTGTCGGTGCCGTGGGTAATGACGAAGCCG                                                                          >  SRR4302537.347252/1‑94 (MQ=60)
                    ACCATCACCACCGGTTTGTCGCATTTCACCGTCAGGTCGAGGAAGTAAGCAGTTTCTTCCATCGTGTCGGTGCCGTGGGTAATGACGAAGCCGT                                                                         <  SRR4302537.3844443/94‑1 (MQ=60)
                    ACCATCACCACCGGTTTGTCGCATTTCACCGTCAGGTCGAGGAAGTAAGCAGTTTCTTCCATCGTGTCGGTGCCGTGGGTAATGACGAAGCCGT                                                                         >  SRR4302537.1568210/1‑94 (MQ=60)
                    ACCATCACCACCGGTTTGTCGCATTTCACCGTCAGGTCGAGGAAGTAAGCAGTTTCTTCCATCGTGTCGGTGCCGTGGGTAATGACGAAGCCGT                                                                         >  SRR4302537.631677/1‑94 (MQ=60)
                     CCATCACCACCGGTTTGTCGCATTTCACCGTCAGGTCGAGGAAGTAAGCAGTTTCTTCCATCGTGTCGGTGCCGTGGGTAATGACGAAGCCGTC                                                                        >  SRR4302537.5198220/1‑94 (MQ=60)
                     CCATCACCACCGGTTTGTCGCATTTCACCGTCAGGTCGAGGAAGTAAGCAGTTTCTTCCATCGTGTCGGTGCCGTGGGTAATGACGAAGCCGTC                                                                        >  SRR4302537.2164537/1‑94 (MQ=60)
                      CATCACCACCGGTTTGTCGCATTTCACCGTCAGGTCGAGGAAGTAAGCAGTTTCTTCCATCGTGTCGGTGCCGTGGGTAATGACGAAGCCGTCG                                                                       <  SRR4302537.4549926/94‑1 (MQ=60)
                      CATCACCACCGGTTTGTCGCATTTCACCGTCAGGTCGAGGAAGTAAGCAGTTTCTTCCATCGTGTCGGTGCCGTGGGTAATGACGAAGCCGTCG                                                                       >  SRR4302537.4312375/1‑94 (MQ=60)
                      CATCACCACCGGTTTGTCGCATTTCACCGTCAGGTCGAGGAAGTAAGCAGTTTCTTCCATCGTGTCGGTGCCGTGGGTAATGACGAAGCCGTCG                                                                       >  SRR4302537.5343464/1‑94 (MQ=60)
                      CATCACCACCGGTTTGTCGCATTTCACCGTCAGGTCGAGGAAGTAAGCAGTTTCTTCCATCGTGTCGGTGCCGTGGGTAATGACGAAGCCGTCG                                                                       <  SRR4302537.3613894/94‑1 (MQ=60)
                       ATCACCACCGGTTTGTCGCATTTCACCGTCAGGTCGAGGAAGTAAGCAGTTTCTTCCATCGTGTCGGTGCCGTGGGTAATGACGAAGCCGTCGG                                                                      >  SRR4302537.4744403/1‑94 (MQ=60)
                       ATCACCACCGGTTTGTCGCATTTCACCGTCAGGTCGAGGAAGTAAGCAGTTTCTTCCATCGTGTCGGTGCCGTGGGTAATGACGAAGCCGTCGG                                                                      <  SRR4302537.4708088/94‑1 (MQ=60)
                       ATCACCACCGGTTTGTCGCATTTCACCGTCAGGTCGAGGAAGTAAGCAGTTTCTTCCATCGTGTCGGTGCCGTGGGTAATGACGAAGCCGTCGG                                                                      >  SRR4302537.3867792/1‑94 (MQ=60)
                         CACCACCGGTTTGTCGCATTTCACCGTCAGGTCGAGGAAGTAAGCAGTTTCTTCCATCGTGTCGGTGCCGTGGGTAATGACGAAGCCGTCGGTC                                                                    <  SRR4302537.6202625/94‑1 (MQ=60)
                         CACCACCGGTTTGTCGCATTTCACCGTCAGGTCGAGGAAGTAAGCAGTTTCTTCCATCGTGTCGGTGCCGTGGGTAATGACGAAGCCGTCGGTC                                                                    <  SRR4302537.2190942/94‑1 (MQ=60)
                         CACCACCGGTTTGTCGCATTTCACCGTCAGGTCGAGGAAGTAAGCAGTTTCTTCCATCGTGTCGGTGCCGTGGGTAATGACGAAGCCGTCGGTC                                                                    >  SRR4302537.2829712/1‑94 (MQ=60)
                          ACCACCGGTTTGTCGCATTTCACCGTCAGGTCGAGGAAGTAAGCAGTTTCTTCCATCGTGTCGGTGCCGTGGGTAATGACGAAGCCGTCGGTCT                                                                   >  SRR4302537.582035/1‑94 (MQ=60)
                          ACCACCGGTTTGTCGCATTTCACCGTCAGGTCGAGGAAGTAAGCAGTTTCTTCCATCGTGTCGGTGCCGTGGGTAATGACGAAGCCGTCGGGCT                                                                   >  SRR4302537.696513/1‑94 (MQ=60)
                           CCACCGGTTTGTCGCATTTCACCGTCAGGTCGAGGAAGTAAGCAGTTTCTTCCATCGTGTCGGTGCCGTGGGTAATGACGAAGCCGTCGGTCTT                                                                  <  SRR4302537.1007535/94‑1 (MQ=60)
                           CCACCGGTTTGTCGCATTTCACCGTCAGGTCGAGGAAGTAAGCAGTTTCTTCCATCGTGTCGGTGCCGTGGGTAATGACGAAGCCGTCGGTCTT                                                                  >  SRR4302537.98077/1‑94 (MQ=60)
                            CACCGGTTTGTCGCATTTCACCGTCAGGTGGAGGAAGTAAGCAGTTTCTTCCATCGTGTCGGTGCCGTGGGTAATGACGAAGCCGTCGGTCTTA                                                                 <  SRR4302537.4584017/94‑1 (MQ=60)
                            CACCGGTTTGTCGCATTTCACCGTCAGGTCGAGGAAGTAAGCAGTTTCTTCCATCGTGTCGGTGCCGTGGGTAATGACGAAGCCGTCGGTCTTA                                                                 >  SRR4302537.2084422/1‑94 (MQ=60)
                             cccgtTTTGTCGCATTTCACCGTCAGGTCGAGGAAGTAAGCAGTTTCTTCCATCGTGTCGGTGCCGTGGGTAATGACGAAGCCGTCGGTCTTAT                                                                <  SRR4302537.2786313/89‑1 (MQ=60)
                             TCCGGTTTGTCGCGTTTCACCGTCAGGTCGAGGAAGTAAGCAGTTTCTTCCATCGTGTCGGTGCCGTTGGTAATGACGAAGCCGTCGGTCTTAT                                                                <  SRR4302537.2530344/94‑1 (MQ=60)
                             ACCGGTTTGTCGCATTTCACCGTCAGGTCGAGGAAGTAAGCAGTTTCTTCCATCGTGTCGGTGCCGTGGGTAATGACGAAGCCGTCGGTCTTAT                                                                <  SRR4302537.3779291/94‑1 (MQ=60)
                              CCGGTTTGTCGCATTTCACCGTCAGGTCGAGGAAGTAAGCAGTTTCTTCCATCGTGTCGGTGCCGTGGGTAATGACGAAGCCGTCGGTCTTATC                                                               >  SRR4302537.98245/1‑94 (MQ=60)
                               CGGTTTGTCGCATTTCACCGTCAGGTCGAGGAAGTAAGCAGTTTCTTCCATCGTGTCGGTGCCGTGGGTAATGACGAAGCCGTCGGTCTTATCG                                                              <  SRR4302537.454904/94‑1 (MQ=60)
                                GGTTTGTCGCATTTCACCGTCAGGTCGAGGAAGTAAGCAGTTTCTTCCATCGTGTCGGTGCCGTGGGTAATGACGAAGCCGTCGGTCTTATCGC                                                             >  SRR4302537.2229173/1‑94 (MQ=60)
                                GGTTTGTCGCATTTCACCGTCAGGTCGAGGAAGTAAGCAGTTTCTTCCATCGTGTCGGTGCCGTGGGTAATGACGAAGCCGTCGGTCTTATCGC                                                             >  SRR4302537.4670760/1‑94 (MQ=60)
                                GGTTTGTCGCATTTCACCGTCAGGTCGAGGAAGTAAGCAGTTTCTTCCATCGTGTCGGTGCCGTGGGTAATGACGAAGCCGTCGGTCTTATCGC                                                             >  SRR4302537.4349773/1‑94 (MQ=60)
                                  TTTGTCGCATTTCACCGTCAGGTCGAGGAAGTAAGCAGTTTCTTCCATCGTGTCGGTGCCGTGGGTAATGACGAAGCCGTCGGTCTTATCGCAG                                                           >  SRR4302537.2328391/1‑94 (MQ=60)
                                  TTTGTCGCATTTCACCGTCAGGTCGAGGAAGTAAGCAGTTTCTTCCATCGTGTCGGTGCCGTGGGTAATGACGAAGCCGTCGGTCTTATCGCAG                                                           <  SRR4302537.3296411/94‑1 (MQ=60)
                                  TTTGTCGCATTTCACCGTCAGGTCGAGGAAGTAAGCAGTTTCTTCCATCGTGTCGGTGCCGTGGGTAATGACGAAGCCGTCGGTCTTATCGCAG                                                           >  SRR4302537.3721541/1‑94 (MQ=60)
                                   TTGTCGCATTTCACCGTCAGGTCGAGGAAGTAAGCAGTTTCTTCCATCGTGTCGGTGCCGTGGGTAATGACGAAGCCGTCGGTCTTATCGCAGT                                                          <  SRR4302537.2427610/94‑1 (MQ=60)
                                   TTGTCGCATTTCACCGTCAGGTCGAGGAAGTAAGCAGTTTCTTCCATCGTGTCGGTGCCGTGGGTAATGACGAAGCCGTCGGTCTTATCGCAGT                                                          <  SRR4302537.2853108/94‑1 (MQ=60)
                                   TTGTCGCATTTCACCGTCAGGTCGAGGAAGTAAGCAGTTTCTTCCATCGTGTCGGTGCCGTGGGTAATGACGAAGCCGTCGGTCTTATCGCAGC                                                          >  SRR4302537.2211789/1‑94 (MQ=60)
                                    TGTCGCATTTCACCGTCAGGTCGATGAAGTAAGCAGTTTCTTCCATCGTGTCGGTGCCGTGGGTAATGACGAAGCCGTCGGTCTTATCGCAGTG                                                         >  SRR4302537.2088257/1‑94 (MQ=60)
                                     GTCGCATTTCACCGTCAGGTCGAGGAAGTAAGCAGTTTCTTCCATCGTGTCGGTGCCGTGGGTAATGACGAAGCCGTCGGTCTTATCGCAGTCG                                                        >  SRR4302537.4843402/1‑94 (MQ=60)
                                     GTCGCATTTCACCGTCAGGTCGAGGAAGTAAGCAGTTTCTTCCATCGTGTCGGTGCCGTGGGTAATGACGAAGCCGTCGGTCTTATCGCAGTCG                                                        <  SRR4302537.785662/94‑1 (MQ=60)
                                       CGCATTTCACCGTCAGGTCGAGGAAGTAAGCAGTTTCTTCCATCGTGTCGGTGCCGTGGGTAATGACGAAGCCGTCGGTCTTATCGCAGTCGGT                                                      <  SRR4302537.600743/94‑1 (MQ=60)
                                        GCATTTCACCGTCAGGTCGAGGAAGTAAGCAGTTTCTTCCATCGTGTCGGTGCCGTGGGTAATGACGAAGCCGTCGGTCTTATCGCAGTCGGTG                                                     <  SRR4302537.1273012/94‑1 (MQ=60)
                                         CATTTCACCGTCAGGTCGAGGAAGTAAGCAGTTTCTTCCATCGTGTCGGTGCCGTGGGTAATGACGAAGCCGTCGGTCTTATCTCAGTCGGTGT                                                    >  SRR4302537.1542472/1‑94 (MQ=60)
                                         CATTTCACCGTCAGGTCGAGGAAGTAAGCAGTTTCTTCCATCGTGTCGGTGCCGTGGGTAATGACGAAGCCGTCGGTCTTATCGCAGTCGGTGT                                                    <  SRR4302537.2599418/94‑1 (MQ=60)
                                          ATTTCACCGTCAGGTCGAGGAAGTAAGCAGTTTCTTCCATCGTGTCGGTGCCGTGGGTAATGACGAAGCCGTCGGTCTTATCGCAGTCGGTGTT                                                   <  SRR4302537.5141087/94‑1 (MQ=60)
                                          ATTTCACCGTCAGGTCGAGGAAGTAAGCAGTTTCTTCCATCGTGTCGGTGCCGTGGGTAATGACGAAGCCGTCGGTCTTATCGCAGTCGGTGTT                                                   >  SRR4302537.4944332/1‑94 (MQ=60)
                                           TTTCACCGTCAGGTCGAGGAAGTAAGCAGTTTCTTCCATCGTGTCGGTGCCGTGGGTAATGACGAAGCCGTCGGTCTTATCGCAGTCGGTGTTA                                                  <  SRR4302537.3519709/94‑1 (MQ=60)
                                            TTCACCGTCAGGTCGAGGAAGTAAGCAGTTTCTTCCATCGTGTCGGTGCCGTGGGTAATGACGAAGCCGTCGGTCTTATCGCAGTCGGTGTTAA                                                 >  SRR4302537.6124852/1‑94 (MQ=60)
                                            TTCACCGTCAGGTCGAGGAAGTAAGCAGTTTCTTCCATCGTGTCGGTGCCGTGGGTAATGACGAAGCCGTCGGTCTTATCGCAGTCGGTGTTAA                                                 <  SRR4302537.1870427/94‑1 (MQ=60)
                                              CACCGTCAGGTCGAGGAAGTAAGCAGTTTCTTCCATCGTGTCGGTGCCGTGGGTAATGACGAAGCCGTCGGTCTTATCGCAGTCGGTGTTAATT                                               >  SRR4302537.4033391/1‑94 (MQ=60)
                                              CACCGTCAGGTCGAGGAAGTAAGCAGTTTCTTCCATCGTGTCGGTGCCGTGGGTAATGACGAAGCCGTCGGTCTTATCGCAGTCGGTGTTAATT                                               <  SRR4302537.3685348/94‑1 (MQ=60)
                                              CACCGTCAGGTCGAGGAAGTAAGCAGTTTCTTCCATCGTGTCGGTGCCGTGGGTAATGACGAAGCCGTCGGTCTTATCGCAGTCGGTGTTAATT                                               <  SRR4302537.1520623/94‑1 (MQ=60)
                                              CACCGTCAGGTCGAGGAAGTAAGCAGTTTCTTCCATCGTGTCGGTGCCGTGGGTAATGACGAAGCCGTCGGTCTTATCGCAGTCGGTGTTAATT                                               >  SRR4302537.2389696/1‑94 (MQ=60)
                                               CCCGTCAGGTCGAGGAAGTAAGCAGTTTCTTCCATCGTGTCGGTGCCGTGGGTAATGACGAAGCCGTCGGTCTTATCGCAGTCGGTGTTAATTT                                              <  SRR4302537.18410/94‑1 (MQ=60)
                                               ACCGTCAGGTCGAGGAAGTAAGCAGTTTCTTCCATCGTGTCGGTGCCGTGGGTAATGACGAAGCCGTCGGTCTTATCGCAGTCGGTGTTAATTT                                              <  SRR4302537.6015214/94‑1 (MQ=60)
                                                CCGTCAGGTCGAGGAAGTAAGCAGTTTCTTCCATCGTGTCGGTGCCGTGGGTAATGACGAAGCCGTCGGTCTTATCGCAGTCGGTGTTAATTTT                                             <  SRR4302537.5082809/94‑1 (MQ=60)
                                                CCGTCAGGTCGAGGAAGTAAGCAGTTTCTTCCATCGTGTCGGTGCCGTGGGTAATGACGAAGCCGTCGGTCTTATCGCAGTCGGTGTTAATTTT                                             >  SRR4302537.5567323/1‑94 (MQ=60)
                                                 CGTCAGGTCGAGGAAGTAAGCAGTTTCTTCCATCGTGTCGGTGCCGTGGGTAATGACGCAGCCGTCGGTCTTATCGCAGTGGGTGTTAATTTTT                                            >  SRR4302537.1070063/1‑94 (MQ=60)
                                                 CGTCAGGTCGAGGAAGTAAGCAGTTTCTTCCATCGTGTCGGTGCCGTGGGTAATGACGAAGCCGTCGGTCTTATCGCAGTCGGTGTTAATTTTT                                            <  SRR4302537.2137153/94‑1 (MQ=60)
                                                 CGTCAGGTCGAGGAAGTAAGCAGTTTCTTCCATCGTGTCGGTGCCGTGGGTAATGACGAAGCCGTCGGTCTTATCGCAGTCGGTGTTAATTTTT                                            >  SRR4302537.1202733/1‑94 (MQ=60)
                                                  GTCAGGTCGAGGAAGTAAGCAGTTTCTTCCATCGTGTCGGTGCCGTGGGTAATGACGAAGCCGTCGGTCTTATCGCAGTCGGTGTTAATTTTTT                                           >  SRR4302537.2638086/1‑94 (MQ=60)
                                                   TCAGGTCGAGGAAGTAAGCAGTTTCTTCCATCGTGTCGGTGCCGTGGGTAATGACGAAGCCGTCGGTCTTATCGCAGTCGGTGTTAATTTTTTT                                          <  SRR4302537.1394478/94‑1 (MQ=60)
                                                    CAGGTCGAGGAAGTAAGCAGTTTCTTCCATCGTGTCGGTGCCGTGGGTAATGACGAAGCCGTCGGTCTTATCGCAGTCGGTGTTAATTTTTTTC                                         <  SRR4302537.2843993/94‑1 (MQ=60)
                                                    CAGGTCGAGGAAGTAAGCAGTTTCTTCCATCGTGTCGGTGCCGTGGGTAATGACGAAGCCGTCGGTCTTATCGCAGTCGGTGTTAATTTTTTTC                                         <  SRR4302537.626243/94‑1 (MQ=60)
                                                    CAGGTCGAGGAAGTAAGCAGTTTCTTCCATCGTGTCGGTGCCGTGGGTAATGACGAAGCCGTCGGTCTTATCGCAGTCGGTGTTAATTTTTTTC                                         >  SRR4302537.3119332/1‑94 (MQ=60)
                                                     AGGTCGAGGAAGTAAGCAGTTTCTTCCATCGTGTCGGTGCCGTGGGTAATGACGAAGCCGTCGGTCTTATCGCAGTCGGTGTTAATTTTTTTCG                                        <  SRR4302537.5695398/94‑1 (MQ=60)
                                                     AGGTCGAGGAAGTAAGCAGTTTCTTCCATCGTGTCGGTGCCGTGGGTAATGACGAAGCCGTCGGTCTTATCGCAGTCGGTGTTAATTTTTTTCG                                        >  SRR4302537.4050438/1‑94 (MQ=60)
                                                     AGGTCGAGGAAGTAAGCAGTTTCTTCCATCGTGTCGGTGCCGTGGGTAATGACGAAGCCGTCGGTCTTATCGCAGTCGGTGTTAATTTTTTTCG                                        <  SRR4302537.4244167/94‑1 (MQ=60)
                                                     AGGTCGAGGAAGTAAGCAGTTTCTTCCATCGTGTCGGTGCCGTGGGTAATGACGAAGCCGTCGGTCTTATCGCAGTCGGTGTTAATTTTTTTCG                                        >  SRR4302537.490651/1‑94 (MQ=60)
                                                      GGTCGAGGAAGTAAGCAGTTTCTTCCATCGTGTCGGTGCCGTGGGTAATGACGAAGCCGTCGGTCTTATCGCAGTCGGTGTTAATTTTTTTCGC                                       >  SRR4302537.110978/1‑94 (MQ=60)
                                                          GAGGAAGTAAGCAGTTTCTTCCATCGTGTCGGTGCCGTTGGTAATGACGAAGCCGTCGGTCTTATCGCAGTCGGTGTTAATTTTTTTCGCCAGT                                   <  SRR4302537.1772015/94‑1 (MQ=60)
                                                          GAGGAAGTAAGCAGTTTCTTCCATCGTGTCGGTGCCGTGGGTAATGACGAAGCCGTCGGTCTTATCGCAGTCGGTGTTAATTTTTTTCGCCAGT                                   >  SRR4302537.2190812/1‑94 (MQ=60)
                                                           AGGAAGTAAGCAGTTTCTTCCATCGTGTCGGTGCCGTGGGTAATGACGAAGCCGTCGGTCTTATCGCAGTCGGTGTTAATTTTTTTCGCCAGTG                                  <  SRR4302537.6191016/94‑1 (MQ=60)
                                                           AGGAAGTAAGCAGTTTCTTCCATCGTGTCGGTGCCGTGGGTAATGACGAAGCCGTCGGTCTTATCGCAGTCGGTGTTAATTTTTTTCGCCAGTG                                  >  SRR4302537.4008766/1‑94 (MQ=60)
                                                             GAAGTAAGCAGTTTCTTCCATCGTGTCGGTGCCGTGGGTAATGACGAAGCCGTCGGTCTTATCGCAGTCGGTGTTAATTTTTTTCGCCAGTGTC                                <  SRR4302537.2974360/94‑1 (MQ=60)
                                                               AGTAAGCAGTTTCTTCCATCGTGTCGGTGCCGTGGGTAATGACGAAGCCGTCGGTCTTATCGCAGTCGGTGTTAATTTTTTTCGCCAGTGTCAG                              <  SRR4302537.2003561/94‑1 (MQ=60)
                                                                GTAAGCAGTTTCTTCCATCGTGTCGGTGCCGTGGGTAATGACGAAGCCGTCGGTCTTATCGCAGTCGGTGTTAATTTTTTTCGCCAGTGTCAGC                             <  SRR4302537.2645162/94‑1 (MQ=60)
                                                                  AAGCAGTTTCTTCCATCGTGTCGGTGCCGTGGGTAATGACGAAGCCGTCGGTCTTATCGCAGTCGGTGTTAATTTTTTTCTCCAGTGTCAGCCA                           >  SRR4302537.1906906/1‑94 (MQ=60)
                                                                   AGCAGTTTCTTCCATCGTGTCGGTGCCGTGGGTAATGACGAAGCCGTCGGTCTTATCGCAGTCGGTGTTAATTTTTTTCGCCAGTGTCAGCCAG                          <  SRR4302537.47507/94‑1 (MQ=60)
                                                                   AGCAGTTTCTTCCATCGTGTCGGTGCCGTGGGTAATGACGAAGCCGTCGGTCTTATCGCAGTCGGTGTTAATTTTTTTCGCCAGTGTCAGCCAG                          <  SRR4302537.5341434/94‑1 (MQ=60)
                                                                    GCAGTTTCTTCCATCGTGTCGGTGCCGTGGGTAATGACGAAGCCGTCGGTCTTATCGCAGTCGGTGTTAATTTTTTTCGCCAGTGTCAGCCAGA                         <  SRR4302537.3924211/94‑1 (MQ=60)
                                                                      AGTTTCTTCCATCGTGTCGGTGCCGTGGGTAATGACGAAGCCGTCGGTCTTATCGCAGTCGGTGTTAATTTTTTTCGCCAGTGTCAGCCAGACA                       >  SRR4302537.5962766/1‑94 (MQ=60)
                                                                      AGTTTCTTCCATCGTGTCGGTGCCGTGGGTAATGACGAAGCCGTCGGTCTTATCGCAGTCGGTGTTAATTTTTTTCGCCAGTGTCAGCCAGACA                       >  SRR4302537.87294/1‑94 (MQ=60)
                                                                       GTTTCTTCCATCGTGTCGGTGCCGTGGGTAATGACGAAGCCGTCGGTCTTATCGCAGTCGGTGTTAATTTTTTTCGCCAGTGTCAGCCAGACAT                      <  SRR4302537.5456657/94‑1 (MQ=60)
                                                                       GTTTCTTCCATCGTGTCGGTGCCGTGGGTAATGACGAAGCCGTCGGTCTTATCGCAGTCGGCGTTAATTTTTTTCGCCAGTGTCAGCCAGACAT                      >  SRR4302537.1079879/1‑94 (MQ=60)
                                                                        TTTCTTCCATCGTGTCGGTGCCGTGGGTAATGACGAAGCCGTCGGTCTTATCGCAGTCGGTGTTAATTTTTTTCGCCAGTGTCAGCCAGACATT                     <  SRR4302537.4525450/94‑1 (MQ=60)
                                                                         TTCTTCCATCGTGTCGGTGCCGTGGGTAATGACGAAGCCGTCGGTCTTATCGCAGTCGGTGTTAATTTTTTTCGCCAGTGTCAGCCAGACATTA                    >  SRR4302537.2295925/1‑94 (MQ=60)
                                                                          TCTTCCATCGTGTCGGTGCCGTGGGTAATGACGAAGCCGTCGGTCTTATCGCAGTCGGTGTTAATTTTTTTCGCCAGTGTCAGCCAGACATTAT                   <  SRR4302537.6448513/94‑1 (MQ=60)
                                                                          TCTTCCATCGTGTCGGTGCCGTGGGTAATGACGAAGCCGTCGGTCTTATCGCAGTCGGTGTTAATTTTTTTCGCCAGTGTCAGCCAGACATTAT                   <  SRR4302537.5586427/94‑1 (MQ=60)
                                                                          TCTTCCATCGTGTCGGTGCCGTGGGTAATGACGAAGCCGTCGGTCTTATCGCAGTCGGTGTTAATTTTTTTCGCCAGTGTCAGCCAGACATTAT                   <  SRR4302537.1224047/94‑1 (MQ=60)
                                                                          TCTTCCATCGTGTCGGTGCCGTGGGTAATGACGAAGCCGTCGGTCTTATCGCAGTCGGTGTTAATTTTTTTCGCCAGTGTCAGCCAGACATTAT                   <  SRR4302537.3200575/94‑1 (MQ=60)
                                                                          TCTTCCATCGTGTCGGTGCCGTGGGTAATGACGAAGCCGTCGGTCTTATCGCAGTCGGTGTTAATTTTTTTCGCCAGTGTCAGCCAGACATTAT                   >  SRR4302537.2969938/1‑94 (MQ=60)
                                                                          TCTTCCATCGTGTCGGTGCCGTGGGTAATGACGAAGCCGTCGGTCTTATCGCAGTCGGTGTTAATTTTTGTCGCCAGTGTCAGCCAGACATTAT                   <  SRR4302537.2187948/94‑1 (MQ=60)
                                                                           CTTCCATCGTGTCGGTGCCGTGGGTAATGACGAAGCCGTCGGTCTTATCGCAGTCGGTGTTAATTTTTTTCGCCAGTGTCAGCCAGACATTATC                  >  SRR4302537.2330197/1‑94 (MQ=60)
                                                                           CTTCCATCGTGTCGGTGCCGTGGGTAATGACGAAGCCGTCGGTCTTATCGCAGTCGGTGTTAATTTTTTTCGCCAGTGTCAGCCAGACATTATC                  >  SRR4302537.829572/1‑94 (MQ=60)
                                                                           CTTCCATCGTGTCGGTGCCGTGGGTAATGACGAAGCCGTCGGTCTTATCGCAGTCGGTGTTAATTTTTTTCGCCAGTGTCAGCCAGACATTATC                  >  SRR4302537.1657775/1‑94 (MQ=60)
                                                                             TCCATCGTGTCGGTGCCGTGGGTAATGACGAAGCCGTCGGTCTTATCGCAGTCGGTGTTAATTTTTTTCGCCAGTGTCAGCCAGACATTATCGT                <  SRR4302537.3556931/94‑1 (MQ=60)
                                                                              CCATCGTGTCGGTGCCGTGGGTAATGACGAAGCCGTCGGTCTTATCGCAGTCGGTGTTAATTTTTTTCGCCAGTGTCAGCCAGACATTATCGTT               <  SRR4302537.3237659/94‑1 (MQ=60)
                                                                              CCATCGTGTCGGTGCCGTGGGTAATGACGAAGCCGTCGGGCTTATCGCAGTCGGCGTTAATTTGTTTCGCCAGTGTCAGCCAGACATTATCGTT               >  SRR4302537.4712739/1‑94 (MQ=60)
                                                                               CATCGTGTCGGTGCCGTGGGTAATGACGAAGCCGTCGGTCTTATCGCAGTCGGTGTTAATTTTTTTCGCCAGTGTCAGCCAGACATTATCGTTC              >  SRR4302537.6273122/1‑94 (MQ=60)
                                                                               CATCGTGTCGGTGCCGTGGGTAATGACGAAGCCGTCGGTCTTATCGCAGTCGGTGTTAATTTTTTTCGCCAGTGTCAGCCAGACATTATCGTTC              <  SRR4302537.3710666/94‑1 (MQ=60)
                                                                                ATCGTGTCGGTGCCGTGGGTAATGACGAAGCCGTCGGTCTTATCGCAGTCGGTGTTAATTTTTTTCGCCAGTGTCAGCCAGACATTATCGTTCA             >  SRR4302537.883438/1‑94 (MQ=60)
                                                                                ATCGTGTCGGTGCCGTGGGTAATGACGAAGCCGTCGGTCTTATCGCAGTCGGTGTTAATTTTTTTCGCCAGTGTCAGCCAGACATTATCGTTCA             <  SRR4302537.4687085/94‑1 (MQ=60)
                                                                                 TCGTGTCGGTGCCGTGGGTAATGACGAAGCCGTCGGTCTTATCGCAGTCGGTGTTAATTTTTTTCGCCAGTGTCAGCCAGACATTATCGTTCAT            <  SRR4302537.2637885/94‑1 (MQ=60)
                                                                                 TCGTGTCGGTGCCGTGGGTAATGACGAAGCCGTCGGTCTTATCGCAGTCGGTGTTAATTTTTTTCGCCAGTGTCAGCCAGACATTATCGTTCAT            >  SRR4302537.4231552/1‑94 (MQ=60)
                                                                                 TCGTGTCGGTGCCGTGGGTAATGACGAAGCCGTCGGTCTTATCGCAGTCGGTGTTAATTTTTTTCGCCAGTGTCAGCCAGACATTATCGTTCAT            <  SRR4302537.767584/94‑1 (MQ=60)
                                                                                 TCGTGTCGGTGCCGTGGGTAATGACGAAGCCGTCGGTCTTATCGCAGTCGGTGTTAATTTTTTTCGCCAGTGTCAGCCAGACATTATCGTTCAT            <  SRR4302537.6031700/94‑1 (MQ=60)
                                                                                  CGTGTCGGTGCCGTGGGTAATGACGAAGCCGTCGGTCTTATCGCAGTCGGTGTTAATTTTTTTCGCCAGTGTCAGCCAGACATTATCGTTCATG           >  SRR4302537.6479985/1‑94 (MQ=60)
                                                                                  CGTGTCGGTGCCGTGGGTAATGACGAAGCCGTCGGTCTTATCGCAGTCGGTGTTAATTTTTTTCGCCAGTGTCAGCCAGACATTATCGTTCATG           >  SRR4302537.1581932/1‑94 (MQ=60)
                                                                                  CGTGTCGGTGCCGTGGGTAATGACGAAGCCGTCGGTCTTATCGCAGTCGGTGTTAATTTTTTTCGCCAGTGTCAGCCAGACATTATCGTTCATG           >  SRR4302537.531997/1‑94 (MQ=60)
                                                                                  CGTGTCGGTGCCGTGGGTAATGACGAAGCCGTCGGTCTTATCGCAGTCGGTGTTAATTTTTTTCGCCAGTGTCAGCCAGACATTATCGTTCATG           <  SRR4302537.590165/94‑1 (MQ=60)
                                                                                   GTGTCGGTGCCGTGGGTAATGACGAAGCCGTCGGTCTTATCGCAGTCGGTGTTAATTTTTTTCGCCAGTGTCAGCCAGACATTATCGTTCATGT          >  SRR4302537.2948072/1‑94 (MQ=60)
                                                                                    TGTCGGTGCCGTGGGTAATGACGAAGCCGTCGGTCTTATCGCAGTCGGTGTTAATTTTTTTCGCCAGTGTCAGCCAGACATTATCGTTCATGTC         >  SRR4302537.1191130/1‑94 (MQ=60)
                                                                                     GTCGGTGCCGTGGGTAATGACGAAGCCGTCGGTCTTATCGCAGTCGGTGTTAATTTTTTTCGCCAGTGTCAGCCAGACATTATCGTTCATGTCC        <  SRR4302537.5756164/94‑1 (MQ=60)
                                                                                     GTCGGTGCCGTGGGTAATGACGAAGCCGTCGGTCTTATCGCAGTCGGTGTTAATTTTTTTCGCCAGTGTCAGCCAGACATTATCGTTCATGTCC        <  SRR4302537.1941611/94‑1 (MQ=60)
                                                                                       CGGTGCCGTGGGTAATGACGAAGCCGTCGGTCTTATCGCAGTCGGTGTTAATTTTTTTCGCCAGTGTCAGCCAGACATTATCGTTCATGTCCTG      <  SRR4302537.5213142/94‑1 (MQ=60)
                                                                                       CGGTGCCGTGGGTAATGACGAAGCCGTCGGTCTTATCGCAGTCGGTGTTAATTTTTTTCGCCAGTGTCAGCCAGACATTATCGTTCATGTCCTG      <  SRR4302537.4439211/94‑1 (MQ=60)
                                                                                       CGGTGCCGTGGGTAATGACGAAGCCGTCGGTCTTATCGCAGTCGGTGTTAATTTTTTTCGCCAGTGTCAGCCAGACATTATCGTTCATGTCCTG      <  SRR4302537.3023067/94‑1 (MQ=60)
                                                                                        GGTGCCGTGGGTAATGACGAAGCCGTCGGTCTTATCGCAGTCGGTGTTAATTTTTTTCGCCAGTGTCAGCCAGACATTATCGTTCATGTCCTGG     <  SRR4302537.1498555/94‑1 (MQ=60)
                                                                                        GGTGCCGTGGGTAATGACGAAGCCGTCGGTCTTATCGCAGTCGGTGTTAATTTTTTTCGCCAGTGTCAGCCAGACATTATCGTTCATGTCCTGG     >  SRR4302537.1921776/1‑94 (MQ=60)
                                                                                         GTGCCGTGGGTAATGACGAAGCCGTCGGTCTTATCGCAGTCGGTGTTAATTTTTTTCGCCAGTGTCAGCCAGACATTATCGTTCATGTCCTGGG    >  SRR4302537.4478665/1‑94 (MQ=60)
                                                                                         GTGCCGTGGGTAATGACGAAGCCGTCGGTCTTATCGCAGTCGGTGTTAATTTTTTTCGCCAGTGTCAGCCAGACATTATCGTTCATGTCCTGGG    <  SRR4302537.3450923/94‑1 (MQ=60)
                                                                                         GTGCCGTGGGTAATGACGAAGCCGTCGGTCTTATCGCAGTCGGTGTTAATTTTTTTCGCCAGTGTCAGCCAGACATTATCGTTCATGTCCTGGG    <  SRR4302537.1254105/94‑1 (MQ=60)
                                                                                           GCCGTGGGTAATGACGAAGCCGTCGGTCTTATCGCAGTCGGTGTTAATTTTTTTCGCCAGTGTCAGCCAGACATTATCGTTCATGTCCTGGGAG  <  SRR4302537.1286899/94‑1 (MQ=60)
                                                                                           |                                                                                             
TGGACGGACGCATTGCGCCGACCATCACCACCGGTTTGTCGCATTTCACCGTCAGGTCGAGGAAGTAAGCAGTTTCTTCCATCGTGTCGGTACCGTGGGTAATGACGAAGCCGTCGGTCTTATCGCAGTCGGTGTTAATTTTTTTCGCCAGTGTCAGCCAGACATTATCGTTCATGTCCTGGGAG  >  NC_000913/3098327‑3098511

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 6 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑