Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I2 R1 882 254.4 12957588 98.0% 12698436 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,127,865 T→C A122A (GCA→GCG)  b2981 ← hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,127,8650TC99.6% 875.5 / ‑5.4 251A122A (GCA→GCG) b2981hypothetical protein
Reads supporting (aligned to +/- strand):  ref base T (0/0);  major base C (133/117);  minor base A (1/0);  total (134/117)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.70e-01

Only 100 of 267 total aligned reads displayed.

TTCAATCAGTAATCCGCTTTCCTGATTGATATTCAGGCTGAAAGGACCGCTGATCTTACTTGCACCTTGTGACTTCAACCACGCTTCCGCTGCGCCAAACAACGCGGCAAAAACCTGCGGATCATCAATGGCGTCAATCATGCCGAAATGACCGGTATCTTTGCCGTAACGCTCGCGGTG  >  NC_000913/3127775‑3127954
                                                                                          |                                                                                         
ttCAATCAGTAATCCGCTTTCCTGATTGATATTCAGGCTGAAAGGACCGCTGATCTTACTTGCACCTTGTGACTTCAACCACGCTTCcgccgc                                                                                         <  2:3543508/93‑1 (MQ=255)
ttCAATCAGTAATCCGCTTTCCTGATTGATATTCAGGCTGAAAGGACCGCTGATCTTACTTGCACCTTGTGACTTCAACCACGCTTCcgccgc                                                                                         >  1:6175996/1‑93 (MQ=255)
 taaaTCAGTAATCCGCTTTCCTGATTGATATTCAGGCTGAAAGGACCGCTGATCTTACTTGCACCTTGTGACTTCAACCACGCTTCCGCcgcg                                                                                        <  1:2216152/91‑1 (MQ=255)
 tCAATCAGTAATCCGCTTTCCTGATTGATATTCAGGCTGAAAGGACCGCTGATCTTACTTGCACCTTGTGACTTCAACCACGCTTCCGCcgcg                                                                                        >  2:4700949/1‑93 (MQ=255)
 tCAATCAGTAATCCGCTTTCCTGATTGATATTCAGGCTGAAAGGACCGCTGATCTTACTTGCACCTTGTGACTTCAACCACGCTTCCGCcgcg                                                                                        >  2:2693960/1‑93 (MQ=255)
    aTCAGTAATCCGCTTTCCTGATTGATATTCAGGCTGAAAGGACCGCTGATCTTACTTGCACCTTGTGACTTCAACCACGCTTCCGCCGCGCCa                                                                                     >  1:944274/1‑93 (MQ=255)
       aGTAATCCGCTTTCCTGATTGATATTCAGGCTGAAAGGACCGCTGATCTTACTTGCACCTTGTGACTTCAACCACGCTTCCGCCGCGCCAaac                                                                                  <  2:3950426/93‑1 (MQ=255)
       aGTAATCCGCTTTCCTGATTGATATTCAGGCTGAAAGGACCGCTGATCTTACTTGCACCTTGTGACTTCAACCACGCTTCCGCCGCGCCAaac                                                                                  <  2:4107929/93‑1 (MQ=255)
        gTAATCCGCTTTCCTGATTGATATTCAGGCTGAAAGGACCGCTGATCTTACTTGCACCTTGTGACTTCAACCACGCTTCCGCCGCGCCAaaca                                                                                 >  1:6349548/1‑93 (MQ=255)
          aaTCCGCTTTCCTGATTGATATTCAGGCTGAAAGGACCGCTGATCTTACTTGCACCTTGTGACTTCAACCACGCTTCCGCCGCGCCAaacaac                                                                               <  1:5327337/93‑1 (MQ=255)
            tCCGCTTTCCTGATTGATATTCAGGCTGAAAGGACCGCTGATCTTACTTGCACCTTGTGACTTCAACCACGCTTCCGCCGCGCCAAACAAcgc                                                                             >  2:6264656/1‑93 (MQ=255)
            tCCGCTTTCCTGATTGATATTCAGGCTGAAAGGACCGCTGATCTTACTTGCACCTTGTGACTTCAACCACGCTTCCGCCGCGCCAAACAAcgc                                                                             >  1:4766702/1‑93 (MQ=255)
             ccGCTTTCCTGATTGATATTCAGGCTGAAAGGACCGCTGATCTTACTTGCACCTTGTGACTTCAACCACGCTTCCGCCGCGCCAAACAAcgcg                                                                            >  2:1421862/1‑93 (MQ=255)
               gCTTTCCTGATTGATATTCAGGCTGAAAGGACCGCTGATCTTACTTGCACCTTGTGACTTCAACCACGCTTCCGCCGCGCCAAACAAcgccgc                                                                          >  1:4424344/1‑93 (MQ=255)
               gCTTTCCTGATTGATATTCAGGCTGAAAGGACCGCTGATCTTACTTGCACCTTGTGACTTCAACCACGCTTCCGCCGCGCCAAACAACGCGGc                                                                          >  2:3857435/1‑93 (MQ=255)
               gCTTTCCTGATTGATATTCAGGCTGAAAGGACCGCTGATCTTACTTGCACCTTGTGACTTCAACCACGCTTCCGCCGCGCCAAACAACGCGGc                                                                          >  1:549729/1‑93 (MQ=255)
                   tCCTGATTGATATTCAGGCTGAAAGGACCGCTGATCTTACTTGCACCTTGTGACTTCAACCACGCTTCCGCCGCGCCAAACAACGCGGCaaaa                                                                      <  1:5373416/93‑1 (MQ=255)
                   tCCTGATTGATATTCAGGCTGAAAGGACCGCTGATCTTACTTGCACCTTGTGACTTCAACCACGCTTCCGCCGCGCCAAACAACGCGGCaaaa                                                                      <  1:488121/93‑1 (MQ=255)
                    ccTGATTGATATTCATGCTGAAAGGACCGCTGATCTTACTTGCACCTTGTGACTTCAACCACGCTTCCGCCGCGCCAAACAACGCGGCaaaaa                                                                     >  2:3211955/1‑93 (MQ=255)
                    ccTGATTGATATTCAGGCTGAAAGGACCGCTGATCTTACTTGCACCTTGTGACTTCAACCACGCTTCCGCCGCGCCAAACAACGCGGCaaaaa                                                                     >  1:5710438/1‑93 (MQ=255)
                     cTGATTGATATTCAGGCTGAAAGGACCGCTGATCTTACTTGCACCTTGTGACTTCAACCACGCTTCCGCCGCGCCAAACAACGCGGCAAAAAc                                                                    >  2:2172166/1‑93 (MQ=255)
                      tgattgatATTCAGGCTGAAAGGACCGCTGATCTTACTTGCACCTTGTGACTTCAACCACGCTTCCGCCGCGCCAAACAACGCGGCAAAAAcc                                                                   >  1:551161/1‑93 (MQ=255)
                       gattgatATTCAGGCTGAAAGGACCGCTGATCTTACTTGCACCTTGTGACTTCAACCACGCTTCCGCCGCGCCAAACAACGCGGCAAAAACCt                                                                  <  2:3610921/93‑1 (MQ=255)
                       gattgatATTCAGGCTGAAAGGACCGCTGATCTTACTTGCACCTTGTGACTTCAACCACGCTTCCGCCGCGCCAAACAACGCGGCAAAAACCt                                                                  >  1:2928238/1‑93 (MQ=255)
                        attgatATTCAGGCTGAAAGGACCGCTGATCTTACTTGCACCTTGTGACTTCAACCACGCTTCCGCCGCGCCAAACAACGCGGCAAAAACCTg                                                                 >  2:5890083/1‑93 (MQ=255)
                        attgatATTCAGGCTGAAAGGACCGCTGATCTTACTTGCACCTTGTGACTTCAACCACGCTTCCGCCGCGCCAAACAACGCGGCAAAAACCTg                                                                 >  2:3676701/1‑93 (MQ=255)
                         ttgatATTCAGGCTGAAAGGACCGCTGATCTTACTTGCACCTTGTGACTTCAACCACGCTTCCGCCGCGCCAAACAACGCGGCAAAAACCTGc                                                                >  1:3210814/1‑93 (MQ=255)
                          tgatATTCAGGCTGAAAGGACCGCTGATCTTACTTGCACCTTGTGACTTCAACCACGCTTCCGCCGCGCCAAACAACGCGGCAAAAACCTGCg                                                               <  2:2345402/93‑1 (MQ=255)
                          tgatATTCAGGCTGAAAGGACCGCTGATCTTACTTGCACCTTGTGACTTCAACCACGCTTCCGCCGCGCCAAACAACGCGGCAAAAACCTGCg                                                               >  2:5415788/1‑93 (MQ=255)
                           gatATTCAGGCTGAAAGGACCGCTGATCTTACTTGCACCTTGTGACTTCAACCACGCTTCCGCCGCGCCAAACAACGCGGCAAAAACCTGCga                                                              >  1:608573/1‑92 (MQ=255)
                            atatTCAGGCTGAAAGGACCGCTGATCTTACTTGCACCTTGTGACTTCAACCACGCTTCCGCCGCGCCAAACAACGCGGCAAAAACCTGCGGa                                                             <  1:1941767/93‑1 (MQ=255)
                             tatTCAGGCTGAAAGGACCGCTGATCTTACTTGCACCTTGTGACTTCAACCACGCTTCCGCCGCGCCAAACAACGCGGCAAAAACCTGCGGat                                                            >  1:2280012/1‑93 (MQ=255)
                             tatTCAGGCTGAAAGGACCGCTGATCTTACTTGCACCTTGTGACTTCAACCACGCTTCCGCCGCGCCAAACAACGCGGCAAAAAACTGCGGat                                                            >  1:1166112/1‑93 (MQ=255)
                              atTCAGGCTGAAAGGACCGCTGATCTTACTTGCACCTTGTGACTTCAACCACGCTTCCGCCGCGCCAAACAACGCGGCAAAAACCTGCGGatc                                                           <  2:4230980/93‑1 (MQ=255)
                               ttCAGGCTGAAAGGACCGCTGATCTTACTTGCACCTTGTGACTTCAACCACGCTTCCGCCGCGCCAAACAACGCGGCAAAAACCTGCGGatca                                                          >  2:620225/1‑93 (MQ=255)
                                tCAGGCTGAAAGGACCGCTGATCTTACTTGCACCTTGTGACTTCAACCACGCTTCCGCCGCGCCAAACAACGCGGCAAAAACCTGCGGatcat                                                         >  1:3404952/1‑93 (MQ=255)
                                   ggCTGAAAGGACCGCTGATCTTACTTGCACCTTGTGACTTCAACCACGCTTCCGCCGCGCCAAACAACGCGGCAAAAACCTGCGGATCATCaa                                                      <  2:2793091/93‑1 (MQ=255)
                                   ggCTGAAAGGACCGCTGATCTTACTTGCACCTTGTGACTTCAACCACGCTTCCGCCGCGCCAAACAACGCGGCAAAAACCTGCGGATCATCaa                                                      >  1:4171314/1‑93 (MQ=255)
                                     cTGAAAGGACCGGTGATCTTACTTGCACCTTGTGACTTCAACCACGTTTCCGCCGCGCCAAACAACTCGGCAAAAACCTGCGGATCATCAATg                                                    <  2:4413761/93‑1 (MQ=255)
                                     cTGAAAGGACCGCTGATCTTACTTGCACCTTGTGACTTCAACCACGCTTCCGCCGCGCCAAACAACGCGGCAAAAACCTGCGGATCATCAATg                                                    <  2:1789915/93‑1 (MQ=255)
                                       gAAAGGACCGCTGATCTTACTTGCACCTTGTGACTTCAACCACGCTTCCGCCGCGCCAAACAACGCGGCAAAAACCTGCGGATCATCAATGGc                                                  <  1:1823730/93‑1 (MQ=255)
                                       gAAAGGACCGCTGATCTTACTTGCACCTTGTGACTTCAACCACGCTTCCGCCGCGCCAAACAACGCGGCAAAAACCTGCGGATCATCAATCGc                                                  >  1:2657914/1‑93 (MQ=255)
                                        aaaGGACCGCTGATCTTACTTGCACCTTGTGACTTCAACCACGCTTCCGCCGCGCCAAACAACGCGGCAAAAACCTGCGGATCATCAATGGCg                                                 >  2:2477193/1‑93 (MQ=255)
                                        aaaGGACCGCTGATCTTACTTGCACCTTGTGACTTCAACCACGCTTCCGCCGCGCCAAACAACGCGGCAAAAACCTGCGGATCATCAATGGCg                                                 >  2:4957172/1‑93 (MQ=255)
                                        aaaGGACCGCTGATCTTACTTGCACCTTGTGACTTCAACCACGCTTCCGCCGCGCCAAACAACGCGGCAAAAACCTGCGGATCATCAATGGCg                                                 >  1:5054162/1‑93 (MQ=255)
                                        aaaGGACCGCTGATCTTACTTGCACCTTGTGACTTCAACCACGCTTCCGCCGCGCCAAACAACGCAGCAAAAACCTGCGGATCATCAATggag                                                 >  1:628046/1‑91 (MQ=255)
                                          aGGACCGCTGATCTTACTTGCACCTTGTGACTTCAACCACGCTTCCGCCGCGCCAAACAACGCGGCAAAAACCTGCGGATCATCAATGGCGTc                                               >  2:5018295/1‑93 (MQ=255)
                                            gACCGCTGATCTTACTTGCACCTTGTGACTTCAACCACGCTTCCGCCGCGCCAAACAACGCGGCAAAAACCTGCGGATCATCAATGGCGTCaa                                             <  1:6006234/93‑1 (MQ=255)
                                            gACCGCTGATCTTACTTGCACCTTGTGACTTCAACCACGCTTCCGCCGCGCCAAACAACGCGGCAAAAACCTGCGGATCATCAATGGCGTCaa                                             <  1:4010691/93‑1 (MQ=255)
                                            gACCGCTGATCTTACTTGCACCTTGTGACTTCAACCACGCTTCCGCCGCGCCAAACAACGCGGCAAAAACCTGCGGATCATCAATGGCGTCaa                                             <  1:4381731/93‑1 (MQ=255)
                                               cGCTGATCTTACTTGCACCTTGTGACTTCAACCACGCTTCCGCCGCGCCAAACAACGCGGCAAAAACCTGCGGATCATCAATGGCGTCAATCa                                          <  2:2629813/93‑1 (MQ=255)
                                               cGCTGATCTTACTTGCACCTTGTGACTTCAACCACGCTTCCGCCGCGCCAAACAACGCGGCAAAAACCTGCGGATCATCAATGGCGTCAATCa                                          <  2:1227770/93‑1 (MQ=255)
                                                gCTGATCTTACTTGCACCTTGTGACTTCAACCACGCTTCCGCCGCGCCAAACAACGCGGCAAAAACCTGCGGATCATCAATGGCGTCAATCAt                                         >  1:675200/1‑93 (MQ=255)
                                                gCTGATCTTACTTGCACCTTGTGACTTCAACCACGCTTCCGCCGCGCCAAACAACGCGGCAAAAACCTGCGGATCATCAATGGCGTCAATCAt                                         <  2:5236202/93‑1 (MQ=255)
                                                 cTGATCTTACTTGCACCTTGTGACTTCAACCACGCTTCCGCCGCGCCAAACAACGCGGCAAAAACCTGCGGATCATCAATGGCGTCAATCATg                                        >  2:3742468/1‑93 (MQ=255)
                                                  tGATCTTACTTGCACCTTGTGACTTCAACCACGCTTCCGCCGCGCCAAACAACGCGGCAAAAACCTGCGGATCATCAATGGCGTCAATCATGc                                       <  2:4202309/93‑1 (MQ=255)
                                                  tGATCTTACTTGCACCTTGTGACTTCAACCACGCTTCCGCCGCGCCAAACAACGCGGCAAAAACCTGCGGATCATCAATGGCGTCAATCATGc                                       >  1:3103992/1‑93 (MQ=255)
                                                   gATCTTACTTGCACCTTGTGACTTCAACCACGCTTCCGCCGCGCCAAACAACGCGGCAAAAACCTGCGGATCATCAATGGCGTCAATCATGcc                                      >  1:3733727/1‑93 (MQ=255)
                                                   gATCTTACTTGCACCTTGTGACTTCAACCACGCTTCCGCCGCGCCAAACAACGCGGCAAAAACCTGCGGATCATCAATGGCGTCAATCATGcc                                      <  1:5217515/93‑1 (MQ=255)
                                                    aTCTTACTTGCACCTTGTGACTTCAACCACGCTTCCGCCGCGCCAAACAACGCGGCAAAAACCTGCGGATCATCAATGGCGTCAATCATGCCg                                     <  1:5852556/93‑1 (MQ=255)
                                                    aTCTTACTTGCACCTTGTGACTTCAACCACGCTTCCGCCGCGCCAAACAACGCGGCAAAAACCTGCGGATCATCAATGGCGTCAATCATGCCg                                     <  2:5532204/93‑1 (MQ=255)
                                                     tCTTACTTGCACCTTGTGACTTCAACCACGCTTCCGCCGCGCCAAACAACGCGGCAAAAACCTGCGGATCATCAATGGCGTCAATCATGCCGa                                    >  2:1133623/1‑93 (MQ=255)
                                                      cTTACTTGCACCTTGTGACTTCAACCACGCTTCCGCCGCGCCAAACAACGCGGCAAAAACCTGCGGATCATCAATGGCGTCAATCATGCCGaa                                   >  1:2795336/1‑93 (MQ=255)
                                                          cTTGCACCTTGTGACTTCAACCACGCTTCCGCCGCGCCAAACAACGCGGCAAAAACCTGCGGATCATCAATGGCGTCAATCATGCCGAAATGa                               >  1:83294/1‑93 (MQ=255)
                                                          cTTGCACCTTGTGACTTCAACCACGCTTCCGCCGCGCCAAACAACGCGGCAAAAACCTGCGGATCATCAATGGCGTCAATCATGCCGAAATGa                               <  1:5606010/93‑1 (MQ=255)
                                                            tGCACCTTGTGACTTCAACCACGCTTCCGCCGCGCCAAACAACGCGGCAAAAACCTGCGGATCATCAATGGCGTCAATCATGCCGAAATGAcc                             >  2:1883554/1‑93 (MQ=255)
                                                             gCACCTTGTGACTTCAACCACGCTTCCGCCGCGCCAAACAACGCGGCAAAAACCTGCGGATCATCAATGGCGTCAATCATGCCGAAATGACCg                            >  1:5634492/1‑93 (MQ=255)
                                                              cACCTTGTGACTTCAACCACGCTTCCGCCGCGCCAAACAACGCGGCAAAAACCTGCGGATCATCAATGGCGTCAATCATGCCGAAATGACCgg                           <  2:2437713/93‑1 (MQ=255)
                                                              cACCTTGTGACTTCAACCACGCTTCCGCCGCGCCAAACAACGCGGCAAAAACCTGCGGATCATCAATGGCGTCAATCATGCCGAAATGACCgg                           >  2:4840475/1‑93 (MQ=255)
                                                               aCCTTGTGACTTCAACCACGCTTCCGCCGCGCCAAACAACGCGGCAAAAACCTGCGGATCATCAATGGCGTCAATCATGCCGAAATGACCGGt                          >  1:950544/1‑93 (MQ=255)
                                                                 cTTGTGACTTCAACCACGCTTCCGCCGCGCCAAACAACGCGGCAAAAACCTGCGGATCATCAATGGCGTCAATCATGCCGAAATGACCGGTAt                        <  2:1836383/93‑1 (MQ=255)
                                                                  ttGTGACTTCAACCACGCTTCCGCCGCGCCAAACAACGCGGCAAAAACCTGCGGATCATCAATGGCGTCAATCATGCAGAAATGACCGGTAtt                       >  1:2097538/1‑92 (MQ=255)
                                                                   tgtgACTTCAACCACGCTTCCGCCGCGCCAAACAACGCGGCAAAAACCTGCGGATCATCAATGGCGTCAATCATGCCGAAATGACCGGTATCt                      >  1:4820674/1‑93 (MQ=255)
                                                                    gtgACTTCAACCACGCTTCCGCCGCGCCAAACAACGCGGCAAAAACCTGCGGATCATCAATGGCGTCAATCATGCCGAAATGACCGGTATCtt                     <  2:231402/93‑1 (MQ=255)
                                                                      gACTTCAACCACGCTTCCGCCGCGCCAAACAACGCGGCAAAAACCTGCGGATCATCAATGGCGTCAATCATGCCGAAATGACCGGTATCTTTg                   <  1:2449723/93‑1 (MQ=255)
                                                                       aCTTCAACCACGCTTCCGCCGCGCCAAACAACGCGGCAAAAACCTGCGGATCATCAATGGCGTCAATCATGCCGAAATGACCGGTATCTTTGc                  <  1:5124812/93‑1 (MQ=255)
                                                                        cTTCAACCACGCTTCCGCCGCGCCAAACAACGCGGCAAAAACCTGCGGATCATCAATGGCGTCAATCATGCCGAAATGACCGGTATCTTTGcc                 >  2:5958468/1‑93 (MQ=255)
                                                                        cTTCAACCACGCTTCCGCCGCGCCAAACAACGCGGCAAAAACCTGCGGATCATCAATGGCGTCAATCATGCCGAAATGACCGGTATCTTTGcc                 >  1:1391838/1‑93 (MQ=255)
                                                                          tCAACCACGCTTCCGCCGCGCCAAAAAACGCGGCAAAAACCTGCGGATCATCAATGGCGTCAATCATGCCGAAAGCACCGGCATCTTTGTCga               >  1:6427543/1‑92 (MQ=255)
                                                                            aaCCACGCTTCCGCCGCGCCAAACAACGCGGCAAAAACCTGCGGATCATCAATGGCGTCAATCATGCCGAAATGACCGGTATCTTTGCCGTaa             <  1:1340553/93‑1 (MQ=255)
                                                                            aaCCACGCTTCCGCCGCGCCAAACAACGCGGCAAAAACCTGCGGATCATCAATGGCGTCAATCATGCCGAAATGACCGGTATCTTTGCCGTaa             <  1:4580798/93‑1 (MQ=255)
                                                                            aaCCACGCTTCCGCCGCGCCAAACAACGCGGCAAAAACCTGCGGATCATCAATGGCGTCAATCATGCCGAAATGACCGGTATCTTTGCCGTaa             >  2:4425266/1‑93 (MQ=255)
                                                                             aCCACGCTTCCGCCGCGCCAAACAACGCGGCAAAAACCTGCGGATCATCAATGGCGTCAATCATGCCGAAATGACCGGTATCTTTGCCGTAAc            <  1:5927753/93‑1 (MQ=255)
                                                                             aCCACGCTTCCGCCGCGCCAAACAACGCGGCAAAAACCTGCGGATCATCAATGGCGTCAATCATGCCGAAATGACCGGTATCTTTGCCGTAAc            <  1:5984613/93‑1 (MQ=255)
                                                                                aCGCTTCCGCCGCGCCAAACAACGCGGCAAAAACCTGCGGATCATCAATGGCGTCAATCATGCCGAAATGACCGGTATCTTTGCCGTAACGCt         >  1:3833230/1‑93 (MQ=255)
                                                                                aCGCTTCCGCCGCGCCAAACAACGCGGCAAAAACCTGCGGATCATCAATGGCGTCAATCATGCCGAAATGACCGGTATCTTTGCCGTAACGCt         <  2:2896191/93‑1 (MQ=255)
                                                                                 cGCTTCCGCCGCGCCAAACAACGCGGCAAAAACCTGCGGATCATCAATGGCGTCAATCATGCCGAAATGACCGGTATCTTTGCCGTAACGCTc        <  1:796892/93‑1 (MQ=255)
                                                                                 cGCTTCCGCCGCGCCAAACAACGCGGCAAAAACCTGCGGATCATCAATGGCGTCAATCATGCCGAAATGACCGGTATCTTTGCCGTAACGCTc        <  2:1528951/93‑1 (MQ=255)
                                                                                 cGCTTCCGCCGCGCCAAACAACGCGGCAAAAACCTGCGGATCATCAATGGCGTCAATCATGCCGAAATGACCGGTATCTTTGCCGTAACGCTc        >  1:5295656/1‑93 (MQ=255)
                                                                                 cGCTTCCGCCGCGCCAAACAACGCGGCAAAAACCTGCGGATCATCAATGGCGTCAATCATGCCGAAATGACCGGTATCTTTGCCGTAACGCTc        <  1:2965267/93‑1 (MQ=255)
                                                                                  gCTTCCGCCGCGCCAAACAACGCGGCAAAAACCTGCGGATCATCAATGGCGTCAATCATGCCGAAATGACCGGTATCTTTGCCGTAACGCTcg       >  2:1334470/1‑93 (MQ=255)
                                                                                  gCTTCCGCCGCGCCAAACAACGCGGCAAAAACCTGCGGATCATCAATGGCGTCAATCATGCCGAAATGACCGGTATCTTTGCCGTAACGCTcg       <  2:3299781/93‑1 (MQ=255)
                                                                                  gCTTCCGCCGCGCCAAACAACGCGGCAAAAACCTGCGGATCATCAATGGCGTCAATCATGCCGAAATGACCGGTATCTTTGCCGTAACGCTcg       <  2:5465600/93‑1 (MQ=255)
                                                                                   cTTCCGCCGCGCCAAACAACGCGTCAAAAACCTGCGGATCATCAATGGCGTCAATCATGCCGAAATGACCGGTATCTTTGCCGTAACGCTcgc      >  2:1172457/1‑93 (MQ=255)
                                                                                   cTTCCGCCGCGCCAAACAACGCGGCAAAAACCTGCGGATCATCAATGGCGTCAATCATGCCGAAATGACCGGTATCTTCGCCGTAACGCTcgc      >  2:2720850/1‑93 (MQ=255)
                                                                                    ttCCGCCGCGCCAAACAACGCGGCAAAAACCTGCGGATCATCAATGGCGTCAATCATGCCGAAATGACCGGTATCTTTGCCGTAACGCTcgcg     >  1:6065887/1‑93 (MQ=255)
                                                                                    ttCCGCCGCGCCAAACAACGCGGCAAAAACCTGCGGATCATCAATGGCGTCAATCATGCCGAAATGACCGGTATCTTTGCCGTAACGCTcgcg     >  1:3496888/1‑93 (MQ=255)
                                                                                    ttCCGCCGCGCCAAACAACGCGGCAAAAACCTGCGGATCATCAATGGCGTCAATCATGCCGAAATGACCGGTATCTTTGCCGTAACGCTcgcg     <  1:234033/93‑1 (MQ=255)
                                                                                      ccgccgCGCCAAACAACGCGGCAAAAACCTGCGGATCATCAATGGCGTCAATCATGCCGAAATGACCGGTATCTTTGCCGTAACGCTCGCGGt   >  1:2118307/1‑93 (MQ=255)
                                                                                       cgccgcGCCAAACAACGCGGCAAAAACCTGCGGATCATCAATGGCGTCAATCATGCCGAAATGACCGGTATCTTTGCCGTAACGCGCGCGGTg  >  1:171500/1‑93 (MQ=255)
                                                                                          |                                                                                         
TTCAATCAGTAATCCGCTTTCCTGATTGATATTCAGGCTGAAAGGACCGCTGATCTTACTTGCACCTTGTGACTTCAACCACGCTTCCGCTGCGCCAAACAACGCGGCAAAAACCTGCGGATCATCAATGGCGTCAATCATGCCGAAATGACCGGTATCTTTGCCGTAACGCTCGCGGTG  >  NC_000913/3127775‑3127954

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
CTTCAATCAGTAATCCGCTTTCCTGATTGATATTCAGGCTGAAAGGACCGCTGATCTTACTTGCACCTTGTGACTTCAACCACGCTTCCGCTGCGCCAAACAACGCGGCAAAAACCTGCGGATCATCAATGGCGTCAATCATGCCGAAATGACCGGTATCTTTGCCGTAACGCTCGCGGTGCAAG  >  NC_000913/3127774‑3127958
                                                                                           |                                                                                             
CTTCAATCAGTAATCCGCTTTCCTGATTGATATTCAGGCTGAAAGGACCGCTGATCTTGCTTGCACCTTGTGACTTCAACCACGCTTCCGCCGC                                                                                             >  SRR4302537.3162337/1‑94 (MQ=60)
CTTCAATCAGTAATCCGCTTTCCTGATTGATATTCAGGCTGAAAGGACCGCTGATCTTACTTGCACCTTGTGACTTCAACCACGCTTCCGCCGC                                                                                             >  SRR4302537.6218532/1‑94 (MQ=60)
  TAAATCAGTAATCCGCTTTCCTGATTGATATTCAGGCTGAAAGGACCGCTGATCTTACTTGCACCTTGTGACTTCAACCACGCTTCCGCCGCGC                                                                                           <  SRR4302537.2231777/94‑1 (MQ=60)
   CAATCAGTAATCCGCTTTCCTGATTGATATTCAGGCTGAAAGGACCGCTGATCTTACTTGCACCTTGTGACTTCAACCACGCTTCCGCCGCGCC                                                                                          <  SRR4302537.2633544/94‑1 (MQ=60)
    AATCAGTAATCCGCTTTCCTGATTGATATTCAGGCTGAAAGGACCGCTGATCTTACTTGCACCTTGTGACTTCAACCACGCTTCCGCCGCGCCA                                                                                         >  SRR4302537.950798/1‑94 (MQ=60)
     ATCAGTAATCCGCTTTCCTGATTGATATTCAGGCTGAAAGGACCGCTGATCTTACTTGCACCTTGTGACTTCAACCACGCTTCCGCCGCGCCAA                                                                                        >  SRR4302537.4450911/1‑94 (MQ=60)
     ATCAGTAATCCGCTTTCCTGATTGATATTCAGGCTGAAAGGACCGCTGATCTTACTTGCACCTTGTGACTTCAACCACGCTTCCGCCGCGCCAA                                                                                        >  SRR4302537.2990148/1‑94 (MQ=60)
     ATCAGTAATCCGCTTTCCTGATTGATATTCAGGCTGAAAGGACCGCTGATCTTACTTGCACCTTGTGACTTCAACCACGCTTCCGCCGCGCCAA                                                                                        <  SRR4302537.6312940/94‑1 (MQ=60)
        AGTAATCCGCTTTCCTGATTGATATTCAGGCTGAAAGGACCGCTGATCTTACTTGCACCTTGTGACTTCAACCACGCTTCCGCCGCGCCAAACA                                                                                     >  SRR4302537.6392905/1‑94 (MQ=60)
        AGTAATCCGCTTTCCTGATTGATATTCAGGCTGAAAGGACCGCTGATCTTACTTGCACCTTGTGACTTCAACCACGCTTCCGCCGCGCCAAACA                                                                                     <  SRR4302537.2959190/94‑1 (MQ=60)
        AGTAATCCGCTTTCCTGATTGATATTCAGGCTGAAAGGACCGCTGATCTTACTTGCACCTTGTGACTTCAACCACGCTTCCGCCGCGCCAAACA                                                                                     <  SRR4302537.1930733/94‑1 (MQ=60)
        AGTAATCCGCTTTCCTGATTGATATTCAGGCTGAAAGGACCGCTGATCTTACTTGCACCTTGTGACTTCAACCACGCTTCCGCCGCGCCAAACA                                                                                     >  SRR4302537.2965548/1‑94 (MQ=60)
        AGTAATCCGCTTTCCTGATTGATATTCAGGCTGAAAGGACCGCTGATCTTACTTGCACCTTGTGACTTCAACCACGCTTCCGCCGCGCCAAACA                                                                                     >  SRR4302537.2114197/1‑94 (MQ=60)
         GTAATCCGCTTTCCTGATTGATATTCAGGCTGAAAGGACCGCTGATCTTACTTGCACCTTGTGACTTCAACCACGCTTCCGCCGCGCCAAACAA                                                                                    >  SRR4302537.6035252/1‑94 (MQ=60)
         GTAATCCGCTTTCCTGATTGATATTCAGGCTGAAAGGACCGCTGATCTTACTTGCACCTTGTGACTTCAACCACGCTTCCGCCGCGCCAAACAA                                                                                    <  SRR4302537.1512710/94‑1 (MQ=60)
          TAATCCGCTTTCCTGATTGATATTCAGGCTGAAAGGACCGCTGATCTTACTTGCACCTTGTGACTTCAACCACGCTTCCGCCGCGCCAAACAAC                                                                                   <  SRR4302537.4014298/94‑1 (MQ=60)
          TAATCCGCTTTCCTGATTGATATTCAGGCTGAAAGGACCGCTGATCTTACTTGCACCTTGTGACTTCAACCACGCTTCCGCCGCGCCAAACAAC                                                                                   >  SRR4302537.5144827/1‑94 (MQ=60)
          TAATCCGCTTTCCTGATTGATATTCAGGCTGAAAGGACCGCTGATCTTACTTGCACCTTGTGACTTCAACCACGCTTCCGCCGCGCCAAACAAC                                                                                   >  SRR4302537.5392976/1‑94 (MQ=60)
           AATCCGCTTTCCTGTTTGATATTCAGGCTGAAAGGACCGCTGATCTTACTTGCACCTTGTGACTTCAACCACGCTTCCGCCGCGCCAAACAACG                                                                                  <  SRR4302537.2371430/94‑1 (MQ=60)
           AATCCGCTTTCCTGATTGATATTCAGGCTGAAAGGACCGCTGATCTTACTTGCACCTTGTGACTTCAACCACGCTTCCGCCGCGCCAAACAACG                                                                                  >  SRR4302537.5160609/1‑94 (MQ=60)
           AATCCGCTTTCCTGATTGATATTCAGGCTGAAAGGACCGCTGATCTTACTTGCACCTTGTGACTTCAACCACGCTTCCGCCGCGCCAAACAACG                                                                                  <  SRR4302537.5363889/94‑1 (MQ=60)
           AATCCGCTTTCCTGATTGATATTCAGGCTGAAAGGACCGCTGATCTTACTTGCACCTTGTGACTTCAACCACGCTTCCGCCGCGCCAAACAACG                                                                                  >  SRR4302537.1295595/1‑94 (MQ=60)
            ATCCGCTTTCCTGATTGATATTCAGGCTGAAAGGACCGCTGATCTTACTTGCACCTTGTGACTTCAACCACGCTTCCGCCGCGCCAAACAACGC                                                                                 >  SRR4302537.6204699/1‑94 (MQ=60)
            ATCCGCTTTCCTGATTGATATTCAGGCTGAAAGGACCGCTGATCTTACTTGCACCTTGTGACTTCAACCACGCTTCCGCCGCGCCAAACAACGC                                                                                 >  SRR4302537.6133668/1‑94 (MQ=60)
            ATCCGCTTTCCTGATTGATATTCAGGCTGAAAGGACCGCTGATCTTACTTGCACCTTGTGACTTCAACCACGCTTCCGCCGCGCCAAACAACGC                                                                                 >  SRR4302537.4799620/1‑94 (MQ=60)
              CCGCTTTCCTGATTGATATTCAGGCTGAAAGGACCGCTGATCTTACTTGCACCTTGTGACTTCAACCACGCTTCCGCCGCGCCAAACAACGCGG                                                                               <  SRR4302537.4490601/94‑1 (MQ=60)
              CCGCTTTCCTGATTGATATTCAGGCTGAAAGGACCGCTGATCTTACTTGCACCTTGTGACTTCAACCACGCTTCCGCCGCGCCAAACAACGCGG                                                                               >  SRR4302537.3778917/1‑94 (MQ=60)
              CCGCTTTCCTGATTGATATTCAGGCTGAAAGGACCGCTGATCTTACTTGCACCTTGTGACTTCAACCACGCTTCCGCCGCGCCAAACAACGCGG                                                                               >  SRR4302537.549531/1‑94 (MQ=60)
              CCGCTTTCCTGATTGATATTCAGGCTGAAAGGACCGCTGATCTTACTTGCACCTTGTGACTTCAACCACGCTTCCGCCGCGCCAAACAACGCGG                                                                               >  SRR4302537.2258191/1‑94 (MQ=60)
               CGCTTTCCTGATTGATATTCAGGCTGAAAGGACCGCTGATCTTACTTGCACCTTGTGACTTCAACCACGCTTCCGCCGCGCCAAACAACGCGGC                                                                              >  SRR4302537.553512/1‑94 (MQ=60)
               CGCTTTCCTGATTGATATTCAGGCTGAAAGGACCGCTGATCTTACTTGCACCTTGTGACTTCAACCACGCTTCCGCCGCGCCAAACAACGCGGC                                                                              >  SRR4302537.5286862/1‑94 (MQ=60)
               CGCTTTCCTGATTGATATTCAGGCTGAAAGGACCGCTGATCTTACTTGCACCTTGTGACTTCAACCACGCTTCCGCCGCGCCAAACAACGCCGC                                                                              >  SRR4302537.4454899/1‑94 (MQ=60)
                GCTTTCCTGATTGATATTCAGGCTGAAAGGACCGCTGATCTTACTTGCACCTTGTGACTTCAACCACGCTTCCTCCGCGCCAAACAACGCGGCA                                                                             <  SRR4302537.2284790/94‑1 (MQ=60)
                GCTTTCCTGATTGATATTCAGGCTGAAAGGACCGCTGATCTTACTTGCACCTTGTGACTTCAACCACGCTTCCGCCGCGCCAAACAACGCGGCA                                                                             <  SRR4302537.951355/94‑1 (MQ=60)
                 CTTTCCTGATTGATATTCAGGCTGAAAGGACCGCTGATCTTACTTGCACCTTGTGACTTCAACCAAGCTTCCGCCGCGCCAAACAACGCGGCAA                                                                            >  SRR4302537.4192406/1‑94 (MQ=60)
                  TTTCCTGATTGATATTCAGGCTGAAAGGACCGCTGATCTTACTTGCACCTTGTGACTTCAACCACGCTTCCGCCGCGCCAAACAACGCGGCAAA                                                                           >  SRR4302537.489778/1‑94 (MQ=60)
                  TTTCCTGATTGATATTCAGGCTGAAAGGACCGCTGATCTTACTTGCACCTTGTGACTTCAACCACGCTTCCGCCGCGCCAAACAACGCGGCAAA                                                                           <  SRR4302537.5482780/94‑1 (MQ=60)
                    TCCTGATTGATATTCAGGCTGAAAGGACCGCTGATCTTACTTGCACCTTGTGACTTCAACCACGCTTCCGCCGCGCCAAACAACGCGGCAAAAA                                                                         <  SRR4302537.5410166/94‑1 (MQ=60)
                    TCCTGATTGATATTCAGGCTGAAAGGACCGCTGATCTTACTTGCACCTTGTGACTTCAACCACGCTTCCGCCGCGCCAAACAACGCGGCAAAAA                                                                         >  SRR4302537.5749523/1‑94 (MQ=60)
                    TCCTGATTGATATTCAGGCTGAAAGGACCGCTGATCTTACTTGCACCTTGTGACTTCAACCACGCTTCCGCCGCGCCAAACAACGCGGCAAAAA                                                                         <  SRR4302537.491474/94‑1 (MQ=60)
                     CCTGATTGATATTCAGGCTGAAAGGACCGCTGATCTTACTTGCACCTTGTGACTTCAACCACGCTTCCGCCGCGCCAAACAACGCGGCAAAAAC                                                                        >  SRR4302537.560478/1‑94 (MQ=60)
                     CCTGATTGATATTCAGGCTGAAAGGACCGCTGATCTTACTTGCACCTTGTGACTTCAACCACGCTTCCGCCGCGCCAAACAACGCGGCAAAAAC                                                                        <  SRR4302537.6441036/94‑1 (MQ=60)
                      CTGATTGATATTCAGGCTGAAAGGACCGCTGATCTTACTTGCACCTTGTGACTTCAACCACGCTTCCGCCGCGCCAAACAACGCGGCAAAAACC                                                                       >  SRR4302537.554952/1‑94 (MQ=60)
                      CTGATTGATATTCAGGCTGAAAGGACCGCTGATCTTACTTGCACCTTGTGACTTCAACCACGCTTCCGCCGCGCCAAACAACGCGGCAAAAACC                                                                       >  SRR4302537.231131/1‑94 (MQ=60)
                      CTGATTGATATTCAGGCTGAAAGGACCGCTGATCTTACTTGCACCTTGTGACTTCAACCACGCTTCCGCCGCGCCAAACAACGCGGCAAAAACC                                                                       >  SRR4302537.841239/1‑94 (MQ=60)
                       TGATTGATATTCAGGCTGAAAGGACCGCTGATCTTACTTGCACCTTGTGACTTCAACCACGCTTCCGCCGCGCCAAACAACGCGGCAAAAACCT                                                                      >  SRR4302537.2948803/1‑94 (MQ=60)
                        GATTGATATTCAGGCTGAAAGGACCGCTGATCTTACTTGCACCTTGTGACTTCAACCACGCTTCCGCCGCGCCAAACAACGCGGCAAAAACCTG                                                                     <  SRR4302537.3656662/94‑1 (MQ=60)
                        GATTGATATTCAGGCTGAAAGGACCGCTGATCTTACTTGCACCTTGTGACTTCAACCACGCTTCCGCCGCGCCAAACAACGCGGCAAAAACCTG                                                                     <  SRR4302537.3733340/94‑1 (MQ=60)
                         ATTGATATTCAGGCTGAAAGGACCGCTGATCTTACTTGCACCTTGTGACTTCAACCACGCTTCCGCCGCGCCAAACAACGCGGCAAAAACCTGC                                                                    <  SRR4302537.2086913/94‑1 (MQ=60)
                         ATTGATATTCAGGCTGAAAGGACCGCTGATCTTACTTGCACCTTGTGACTTCAACCACGCTTCCGCCGCGCCAAACAACGCGGCAAAAACCTGC                                                                    >  SRR4302537.3233397/1‑94 (MQ=60)
                          TTGATATTCAGGCTGAAAGGACCGCTGATCTTTCTTGCACCTTGTGCCTTCAACCACGCTTCCGCCGCGCCAAACAACGCGGCAAAAACCTGCG                                                                   <  SRR4302537.5555420/94‑1 (MQ=60)
                          TTGATATTCAGGCTGAAAGGACCGCTGATCTTACTTGCACCTTGTGACTTCAACCACGCTTCCGCCGCGCCAAACAACGCGGCAAAAACCTGCG                                                                   >  SRR4302537.5793125/1‑94 (MQ=60)
                          TTGATATTCAGGCTGAAAGGACCGCTGATCTTACTTGCACCTTGTGACTTCAACCACGCTTCCGCCGCGCCAAACAACGCGGCAAAAACCTGCG                                                                   >  SRR4302537.1385123/1‑94 (MQ=60)
                           TGATATTCAGGCTGAAAGGACCGCTGATCTTACTTGCACCTTGTGACTTCAACCACGCTTCCGCCGCGCCAAACAACGCGGCAAAAACCTGCGG                                                                  <  SRR4302537.5980071/94‑1 (MQ=60)
                           TGATATTCAGGCTGAAAGGACCGCTGATCTTACTTGCACCTTGTGACTTCAACCACGCTTCCGCCGCGCCAAACAACGCGGCAAAAACCTGCGA                                                                  >  SRR4302537.612732/1‑94 (MQ=60)
                             ATATTCAGGCTGAAAGGACCGCTGATCTTACTTGCACCTTGTGACTTCAACCACGCTTCCGCCGCGCCAAACAACGCGGCAAAAACGTGCGGAC                                                                >  SRR4302537.2767460/1‑94 (MQ=60)
                             ATATTCAGGCTGAAAGGACCGCTGATCTTACTTGCACCTTGTGACTTCAACCACGCTTCCGCCGCGCCAAACAACGCGGCAAAAACCTGCGGAT                                                                <  SRR4302537.855229/94‑1 (MQ=60)
                             ATATTCAGGCTGAAAGGACCGCTGATCTTACTTGCACCTTGTGACTTCAACCACGCTTCCGCCGCGCCAAACAACGCGGCAAAAACCTGCGGAT                                                                <  SRR4302537.1955265/94‑1 (MQ=60)
                             ATATTCAGGCTGAAAGGACCGCTGATCTTACTTGCACCTTGTGACTTCAACCACGCTTCCGCCGCGCCAAACAACGCGGCAAAAACCTGCGGAT                                                                >  SRR4302537.2296103/1‑94 (MQ=60)
                             ATATTCAGGCTGAAAGGACCGCTGATCTTACTTGCACCTTGTGACTTCAACCACGCTTCCGCCGCGCCAAACAACGCGGCAAAAACCTGCGGAT                                                                >  SRR4302537.656156/1‑94 (MQ=60)
                             ATATTCAGGCTGAAAGGACCGCTGATCTTACTTGCACCTTGTGACTTCAACCACGCTTCCGCCGCGCCAAACAACGCGGCAAAAAACTGCGGAT                                                                >  SRR4302537.1174219/1‑94 (MQ=60)
                              TATTCAGGCTGAAAGGACCGCTGATCTTACTTGCACCTTGTGACTTCAACCACGCTTCCGCCGCGCCAAACAACGCGGCAAAAACCTGCGGATC                                                               <  SRR4302537.4857395/94‑1 (MQ=60)
                                TTCAGGCTGAAAGGACCGCTGATCTTACTTGCACCTTGTGACTTCAACCACGCTTCCGCCGCGCCAAACAACGCGGCAAAAACCTGCGGATCAT                                                             >  SRR4302537.3429068/1‑94 (MQ=60)
                                 TCAGGCTGAAAGGACCGCTGATCTTACTTGCACCTTGTGACTTCAACCACGCTTCCGCCGCGCCAAACAACGCGGCAAAAACCTGCGGATCATC                                                            >  SRR4302537.4261441/1‑94 (MQ=60)
                                 TCAGGCTGAAAGGACCGCTGATCTTACTTGCACCTTGTGACTTCAACCACGCTTCCGCCGCGCCAAACAACGCGGCAAAAACCTGCGGATCATC                                                            <  SRR4302537.6097744/94‑1 (MQ=60)
                                 TCAGGCTGAAAGGACCGCTGATCTTACTTGCACCTTGTGACTTCAACCACGCTTCCGCCGCGCCAAACAACGCGGAAAAAACCTGCGGATCATC                                                            <  SRR4302537.683192/94‑1 (MQ=60)
                                   AGGCTGAAAGGACCGCTGATCTTACTTGCACCTTGTGACTTCAACCACGCTTCCGCCGCGCCAAACAACGCGGCAAAAACCTGCGGATCATCAA                                                          >  SRR4302537.4200447/1‑94 (MQ=60)
                                    GGCTGAAAGGACCGCTGATCTTACTTGCACCTTGTGACTTCAACCACGCTTCCGCCGCGCCAAACAACGCGGCAAAAACCTGCGGATCATCAAT                                                         >  SRR4302537.5334277/1‑94 (MQ=60)
                                      CTGAAAGGACCGCTGATCTTACTTGCACCTTGTGACTTCAACCACGCTTCCGCCGCGCCAAACAACGCGGCAAAAACCTGCGGATCATCAATGG                                                       >  SRR4302537.3835861/1‑94 (MQ=60)
                                       TGAAAGGACCGCTGATCTTACTTGCACCTTGTGACTTCAACCACGCTTCCGCCGCGCCAAACAACGCGGCAAAAACCTGCGGATCATCAATGGC                                                      >  SRR4302537.3289623/1‑94 (MQ=60)
                                       TGAAAGGACCGCTGATCTTACTTGCACCTTGTGACTTCAACCACGCTTCCGCCGCGCCAAACAACGCGGCAAAAACCTGCGGATCATCAATCGC                                                      >  SRR4302537.2676488/1‑94 (MQ=60)
                                        GAAAGGACCGCTGATCTTACTTGCACCTTGTGACTTCAACCACGCTTCCGCCGCGCCAAACAACGCGGCAAAAACCTGCGGATCATCAATGGCG                                                     >  SRR4302537.5088958/1‑94 (MQ=60)
                                        GAAAGGACCGCTGATCTTACTTGCACCTTGTGACTTCAACCACGCTTCCGCCGCGCCAAACAACGCGGCAAAAACCTGCGGATCATCAATGGCG                                                     <  SRR4302537.1836441/94‑1 (MQ=60)
                                        GAAAGGACCGCTGATCTTACTTGCACCTTGTGACTTCAACCACGCTTCCGCCGCGCCAAACAACGCAGCAAAAACCTGCGGATCATCAATGGAG                                                     >  SRR4302537.632322/1‑94 (MQ=60)
                                        GAAAGGACCGCTGATCTTACTTGCACCGTGTGACTTCAACCACGCTTCCGCCGCGCCAAACAACGCGGCAAAAACCTGCGGATCATCAATGGCG                                                     <  SRR4302537.5249244/94‑1 (MQ=60)
                                           AGGACCGCTGATCTTACTTGCACCTTGTGACTTCAACCACGCTTCCGCCGCGCCAAACAACGCGGCAAAAACCTGCGGATCATCAATGGCGTCA                                                  >  SRR4302537.3435429/1‑94 (MQ=60)
                                           AGGACCGCTGATCTTACTTGCACCTTGTGACTTCAACCACGCTTCCGCCGCGCCAAACAACGCGGCAAAAACCTGCGGATCATCAATGGCGTCA                                                  <  SRR4302537.4163930/94‑1 (MQ=60)
                                             GACCGCTGATCTTACTTGCACCTTGTGACTTCAACCACGCTTCCGCCGCGCCAAACAACGCGGCAAAAACCTGCGGATCATCAATGGCGTCAAT                                                <  SRR4302537.6047497/94‑1 (MQ=60)
                                             GACCGCTGATCTTACTTGCACCTTGTGACTTCAACCACGCTTCCGCCGCGCCAAACAACGCGGCAAAAACCTGCGGATCATCAATGGCGTCAAT                                                <  SRR4302537.4411972/94‑1 (MQ=60)
                                             GACCGCTGATCTTACTTGCACCTTGTGACTTCAACCACGCTTCCGCCGCGCCAAACAACGCGGCAAAAACCTGCGGATCATCAATGGCGTCAAT                                                <  SRR4302537.4038778/94‑1 (MQ=60)
                                              GCCGCTGATCTTACTTGCACCTTGTGACTTCAACCACGCTTCCGCCGCGCCAAACAACGCGGCAAAAACCTGCGGATCATCAATGGCGTCAATC                                               <  SRR4302537.1395412/94‑1 (MQ=60)
                                                CGCTGATCTTACTTGCACCTTGTGACTTCAACCACGCTTCCGCCGCGCCAAACAACGCGGCAAAAACCTGCGGATCATCAATGGCGTCAATCAT                                             >  SRR4302537.81320/1‑94 (MQ=60)
                                                CGCTGATCTTACTTGCACCTTGTGACTTCAACCACGCTTCCGCCGCGCCAAACAACGCGGCAAAAACCTGCGGATCATCAATGGCGTCAATCAT                                             <  SRR4302537.6054682/94‑1 (MQ=60)
                                                CGCTGATCTTACTTGCACCTTGTGACTTCAACCACGCTTCCGCCGCGCCAAACAACGCGGCAAAAACCTGCGGATCATCAATGGCGTCAATCAT                                             >  SRR4302537.679822/1‑94 (MQ=60)
                                                CGCTGATCTTACTTGCACCTTGTGACTTCAACCACGCTTCCGCCGCGCCAAACAACGCGGCAAAAACCTGCGGATCATCAATGGCGTCAATCAT                                             <  SRR4302537.5588189/94‑1 (MQ=60)
                                                 GCTGATTTTACTTGCACCTTGTGACTTCAACCACGCTTCCGCCGCGCCAAACAACGCGGCAAAAACCTGCGGATCATCAATGGCGTCAATCATG                                            <  SRR4302537.5795028/94‑1 (MQ=60)
                                                 GCTGATCTTACTTGCACCTTGTGACTTCAACCACGCTTCCGCCGCGCCAAACAACGCGGCAAAAACCTGCGGATCATCAATGGCGTCAATCATG                                            <  SRR4302537.216888/94‑1 (MQ=60)
                                                 GCTGATCTTACTTGCACCTTGTGACTTCAACCACGCTTCCGCCGCGCCAAACAACGCGGCAAAAACCTGCGGATCATCAATGGCGTCAATCATG                                            <  SRR4302537.2570949/94‑1 (MQ=60)
                                                 GCTGATCTTACTTGCACCTTGTGACTTCAACCACGCTTCCGCCGCGCCAAACAACGCGGCAAAAACCTGCGGATCATCAATGGCGTCAATCATG                                            >  SRR4302537.4375507/1‑94 (MQ=60)
                                                  CTGATCTTACTTGCACCTTGTGACTTCAACCACGCTTCCGCCGCGCCAAACAACGCGGCAAAAACCTGCGGATCATCAATGGCGTCAATCATGC                                           >  SRR4302537.1472490/1‑94 (MQ=60)
                                                  CTGATCTTACTTGCACCTTGTGACTTCAACCACGCTTCCGCCGCGCCAAACAACGCGGCAAAAACCTGCGGATCATCAATGGCGTCAATCATGC                                           >  SRR4302537.3125672/1‑94 (MQ=60)
                                                   TGATCTTACTTGCACCTTGTGACTTCAACCACGCTTCCGCCGCGCCAAACAACGCGGCAAAAACCTGCGGATCATCAATGGCGTCAATCATGCC                                          >  SRR4302537.3759952/1‑94 (MQ=60)
                                                   TGATCTTACTTGCACCTTGTGACTTCAACCACGCTTCCGCCGCGCCAAACAACGCGGCAAAAACCTGCGGATCATCAATGGCGTCAATCATGCC                                          >  SRR4302537.1996299/1‑94 (MQ=60)
                                                    GATCTTACTTGCACCTTGTGACTTCAACCACGCTTCCGCCGCGCCAAACAACGCGGCAAAAACCTGCGGATCATCAATGGCGTCAATCATGCCG                                         <  SRR4302537.811886/94‑1 (MQ=60)
                                                    GATCTTACTTGCACCTTGTGACTTCAACCACGCTTCCGCCGCGCCAAACAACGCGGCAAAAACCTGCGGATCATCAATGGCGTCAATCATGCCG                                         <  SRR4302537.5253396/94‑1 (MQ=60)
                                                     ATCTTACTTGCACCTTGTGACTTCAACCACGCTTCCGCCGCGCCAAACAACGCGGCAAAAACCTGCGGATCATCAATGGCGTCAATCATGCCGA                                        >  SRR4302537.65168/1‑94 (MQ=60)
                                                     ATCTTACTTGCACCTTGTGACTTCAACCACGCTTCCGCCGCGCCAAACAACGCGGCAAAAACCTGCGGATCATCAATGGCGTCAATCATGCCGA                                        <  SRR4302537.5892703/94‑1 (MQ=60)
                                                      TCTTACTTGCACCTTGTGACTTCAACCACGCTTCCGCCGCGCCAAACAACGCGGCAAAAACCTGCGGATCATCAATGGCGTCAATCATGCCGAA                                       >  SRR4302537.2814879/1‑94 (MQ=60)
                                                       CTTACTTGCACCTTGTGACTTCAACCACGCTTCCGCCGCGCCAAACAACGCGGCAAAAACCTGCGGATCATCAATGGCGTCAATCATGCCGAAA                                      <  SRR4302537.5300574/94‑1 (MQ=60)
                                                        TTACTTGCACCTTGTGACTTCAACCACGCTTCCGCCGCGCCAAACAACGCGGCAAAAACCTGCGGATCATCAATGGCGTCAATCATGCCGAAAT                                     >  SRR4302537.1836661/1‑94 (MQ=60)
                                                        TTACTTGCACCTTGTGACTTCAACCACGCTTCCGCCGCGCCAAACAACGCGGCAAAAACCTGCGGATCATCAATGGCGTCAATCATGCCGAAAT                                     <  SRR4302537.2871634/94‑1 (MQ=60)
                                                          ACTTGCACCTTGTGACTTCAACCACGCTTCCGCCGCGCCAAACAACGCGGCAAAAACCTGCGGATCATCAATGGCGTCAATCATGCCGAAATGA                                   >  SRR4302537.83906/1‑94 (MQ=60)
                                                           tattccgtTTGTGACTTCAACCACGCTTCCGCCGCGCCAAACAACGCGGCAAAAACCTGCGGATCATCAATGGCGTCAATCATGCCGAAATGAC                                  <  SRR4302537.5397192/86‑1 (MQ=60)
                                                           CTTGCACCTTGTGACTTCAACCACGCTTCCGCCGCGCCAAACAACGCGGCAAAAACCTGCGGATCATCAATGGCGTCAATCATGCCGAAATGAC                                  <  SRR4302537.5644338/94‑1 (MQ=60)
                                                           CTTGCACCTTGTGACTTCAACCACGCTTCCGCCGCGCCAAACAACGCGGCAAAAACCTGCGGATCATCAATGGCGTCAATCATGCCGAAATGAC                                  >  SRR4302537.4745360/1‑94 (MQ=60)
                                                           CTTGCACCTTGTGACTTCAACCACGCTTCCGCCGCGCCAAACAACGCGGCAAAAACCTGCGGATCATCAATGGCGTCAATCATGCCGAAATGAC                                  >  SRR4302537.2367094/1‑94 (MQ=60)
                                                             TGCACCTTGTGACTTCAACCACGCTTCCGCCGCGCCAAACAACGCGGCAAAAACCTGCGGATCATCAATGGCGTCAATCATGCCGAAATGACCG                                >  SRR4302537.5673049/1‑94 (MQ=60)
                                                               CACCTTGTGACTTCAACCACGCTTCCGCCGCGCCAAACAACGCGGCAAAAACCTGCGGATCATCAATGGCGTCAATCATGCCGAAATGACCGGT                              >  SRR4302537.957115/1‑94 (MQ=60)
                                                                  CTTGTGACTTCAACCACGCTTCCGCCGCGCCAAACAACGCGGTAAAAACCTGCGGATCATCAATGGCGTCAATCATGCCGAAATGACCGGTATC                           >  SRR4302537.2928161/1‑94 (MQ=60)
                                                                  CTTGTGACTTCAACCACGCTTCCGCCGCGCCAAACAACGCGGCAAAAACCTGCGGATCATCAATGGCGTCAATCATGCCGAAATGACCGGTATC                           >  SRR4302537.627883/1‑94 (MQ=60)
                                                                  CTTGTGACTTCAACCACGCTTCCGCCGCGCCAAACAACGCGGCAAAAACCTGCGGATCATCAATGGCGTCAATCATGCCGAAAGGACCGGgatt                           >  SRR4302537.3745134/1‑90 (MQ=60)
                                                                  CTTGTGACTTCAACCACGCTTCCGCCGCGCCAAACAACGCGGCAAAAACCTGCGGATCATCAATGGCGTCAATCATGCAGAAATGACCGGTATT                           >  SRR4302537.2112332/1‑94 (MQ=60)
                                                                   TTGTGACTTCAACCACGCTTCCGCCGCGCCAAACAACGCGGCAAAAACCTGCGGATCATCAATGGCGTCAATCATGCCGAAATGACCGGTATCT                          <  SRR4302537.893461/94‑1 (MQ=60)
                                                                   TTGTGACTTCAACCACGCTTCCGCCGCGCCAAACAACGCGGCAAAAACCTGCGGATCATCAATGGCGTCAATCATGCCGAAATGACCGGTATCT                          >  SRR4302537.4853974/1‑94 (MQ=60)
                                                                    TGTGACTTCAACCACGCTTCCGCCGCGCCAAACAACGCGGCAAAAACCTGCGGATCATCAATGGCGTCAATCATGCCGAAATGACCGGTATCTT                         >  SRR4302537.3200208/1‑94 (MQ=60)
                                                                     GTGACTTCAACCACGCTTCCGCCGCGCCAAACAACGCGGCAAAAACCTGCGGATCATCAATGGCGTCAATCATGCCGAAATGACCGGTATCTTT                        <  SRR4302537.633046/94‑1 (MQ=60)
                                                                     GTGACTTCAACCACGCTTCCGCCGCGCCAAACAACGCGGCAAAAACCTGCGGATCATCAATGGCGTCAATCATGCCGAAATGACCGGTATCTTT                        >  SRR4302537.5735038/1‑94 (MQ=60)
                                                                      TGACTTCAACCACGCTTCCGCCGCGCCAAACAACGCGGCAAAAACCTGCGGATCATCAATGGCGTCAATCATGCCGAAATGACCGGTATCTTTG                       <  SRR4302537.5548742/94‑1 (MQ=60)
                                                                      TGACTTCAACCACGCTTCCGCCGCGCCAAACAACGCGGCAAAAACCTGCGGATCATCAATGGCGTCAATCATGCCGAAATGACCGGTATCTTTG                       >  SRR4302537.596220/1‑94 (MQ=60)
                                                                       GACTTCAACCACGCTTCCGCCGCGCCAAACAACGCGGCAAAAACCTGCGGATCATCAATGGCGTCAATCATGCCGAAATGACCGGTATCTTTGC                      <  SRR4302537.2466956/94‑1 (MQ=60)
                                                                        ACTTCAACCACGCTTCCGCCGCGCCAAACAACGCGGCAAAAACCTGCGGATCATCAATGGCGTCAATCATGCCGAAATGACCGGTATCTTTGCC                     <  SRR4302537.6419326/94‑1 (MQ=60)
                                                                        ACTTCAACCACGCTTCCGCCGCGCCAAACAACGCGGCAAAAACCTGCGGATCATCAATGGCGTCAATCATGCCGAAATGACCGGTATCTTTGCC                     >  SRR4302537.1401544/1‑94 (MQ=60)
                                                                        ACTTCAACCACGCTTCCGCCGCGCCAAACAACGCGGCAAAAACCTGCGGATCATCAATGGCGTCAATCATGCCGAAATGACCGGTATCTTTGCC                     <  SRR4302537.5160129/94‑1 (MQ=60)
                                                                         CTTCAACCACGCTTCCGCCGCGCCAAACAACGCGGCAAAAACCTGCGGATCATCAATGGCGTCAATCATGCCGAAATGACCGGTATCTTTGCCG                    >  SRR4302537.6110409/1‑94 (MQ=60)
                                                                          TTCAACCACGCTTCCGCCGCGCCAAACAACGCGGCAAAAACCTGCGGATCATCAATGGCGTCAATCATGCCGAAATGACCGGTATCTTTGCCGT                   >  SRR4302537.5649790/1‑94 (MQ=60)
                                                                          TTCAACCACGCTTCCGCCGCGCCAAAAAACGCGGCAAAAACCTGCGGATCATCAATGGCGTCAATCATGCCGAAAgcaccggcatctttgtcga                   >  SRR4302537.6471219/1‑75 (MQ=60)
                                                                             AACCACGCTTCCGCCGCGCCAAGCATCGCGGCAAAAACCTGCGGATCATCAATGGCGTCAATCATGCCGAAATGACCGGTATCTTTGCCGTAAC                <  SRR4302537.3779322/94‑1 (MQ=60)
                                                                             AACCACGCTTCCGCCGCGCCAAACAACGCGGCAAAAACCTGCGGATCATCAATGGCGTCAATCATGCCGAAATGACCGGTATCTTTGCCGTAAC                <  SRR4302537.4612486/94‑1 (MQ=60)
                                                                             AACCACGCTTCCGCCGCGCCAAACAACGCGGCAAAAACCTGCGGATCATCAATGGCGTCAATCATGCCGAAATGACCGGTATCTTTGCCGTAAC                <  SRR4302537.1349888/94‑1 (MQ=60)
                                                                              ACCACGCTTCCGCCGCGCCAAACAACGCGGCAAAAACCTGCGGATCATCAATGGCGTCAATCATGCCGAAATGACCGGTATCTTTGCCGTAACG               <  SRR4302537.6025686/94‑1 (MQ=60)
                                                                              ACCACGCTTCCGCCGCGCCAAACAACGCGGCAAAAACCTGCGGATCATCAATGGCGTCAATCATGCCGAAATGACCGGTATCTTTGCCGTAACG               <  SRR4302537.5968432/94‑1 (MQ=60)
                                                                               CCACGCTTCCGCCGCGCCAAACAACGCGGCAAAAACCTGCGGATCATCAATGGCGTCAATCATGCCGAAATGACCGGTATCTTTGCCGTAACGC              >  SRR4302537.1738716/1‑94 (MQ=60)
                                                                               CCACGCTTCCGCCGCGCCAAACAACGCGGCAAAAACCTGCGGATCATCAATGGCGTCAATCATGCCGAAATGACCGGTATCTTTGCCGTAACGC              >  SRR4302537.477934/1‑94 (MQ=60)
                                                                                CACGCTTCCGCCGCGCCAAACAACGCGGCAAAAACCTGCGGATCATCAATGGCGTCAATCATGCCGAAATGACCGGTATCTTTGCCGTAACGCT             <  SRR4302537.5050665/94‑1 (MQ=60)
                                                                                CACGCTTCCGCCGCGCCAAACAACGCGGCAAAAACCTGCGGATCATCAATGGCGTCAATCATGCCGAAATGACCGGTATCTTTGCCGTAACGCT             >  SRR4302537.3860096/1‑94 (MQ=60)
                                                                                CACGCTTCCGCCGCGCCAAACAACGCGGCAAAAACCTGCGGATCATCAATGGCGTCAATCATGCCGAAATGACCGGTATCTTTGCCGTAACGCT             <  SRR4302537.5660559/94‑1 (MQ=60)
                                                                                 CCGCTTCCGCCGCGCCAAACAACGCGGCAAAAACCTGCGGATCATCAATGGCGTCAATCATGCCGAAATGACCGGTATCTTTGCCGTAACGCTC            <  SRR4302537.481607/94‑1 (MQ=60)
                                                                                 ACGCTTCCGCCGCGCCAAACAACGCGGCAAAAACCTGCGGATCATCAATGGCGTCAATCATGCCGAAATGACCGGTATCTTTGCCGTAACGCTC            >  SRR4302537.5332042/1‑94 (MQ=60)
                                                                                 ACGCTTCCGCCGCGCCAAACAACGCGGCAAAAACCTGCGGATCATCAATGGCGTCAATCATGCCGAAATGACCGGTATCTTTGCCGTAACGCTC            <  SRR4302537.6044321/94‑1 (MQ=60)
                                                                                  CGCTTCCGCCGCGCCAAACAACGCGGCAAAAACCTGCGGATCATCAATGGCGTCAATCATGCCGAAATGACCGGTATCTTTGCCGTAACGCTCG           <  SRR4302537.802361/94‑1 (MQ=60)
                                                                                  CGCTTCCGCCGCGCCAAACAACGCGGCAAAAACCTGCGGATCATCAATGGCGTCAATCATGCCGAAATGACCGGTATCTTTGCCGTAACGCTCG           <  SRR4302537.2986073/94‑1 (MQ=60)
                                                                                  CGCTTCCGCCGCGCCAAACAACGCGGCAAAAACCTGCGGATCATCAATGGCGTCAATCATGCCGAAATGACCGGTATCTTTGCCGTAACGCTCG           <  SRR4302537.496177/94‑1 (MQ=60)
                                                                                   GCTTCCGCCGCGCCAAACAACGCGGCAAAAACCTGCGGATCATCAATGGCGTCAATCATGCCGAAATGACCGGTATCTTTGCCGTAACGCTCGC          <  SRR4302537.453444/94‑1 (MQ=60)
                                                                                    CTTCCGCCGCGCCAAACAACGCGGCAAAAACCTGCGGATCATCAATGGCGTCAATCATGCCGAAATGACCGGTATCTTTGCCGTAACGCTCGCG         <  SRR4302537.5923149/94‑1 (MQ=60)
                                                                                    CTTCCGCCGCGCCAAACAACGCGGCAAAAACCTGCGGATCATCAATGGCGTCAATCATGCCGAAATGACCGGTATCTTTGCCGTAACGCTCGCG         >  SRR4302537.3521626/1‑94 (MQ=60)
                                                                                    CTTCCGCCGCGCCAAACAACGCGGCAAAAACCTGCGGATCATCAATGGCGTCAATCATGCCGAAATGACCGGTATCTTTGCCGTAACGCTCGCG         >  SRR4302537.6107557/1‑94 (MQ=60)
                                                                                     TTCCGCCGCGCCAAACAACGCGGCAAAAACCTGCGGATCATCAATGGCGTCAATCATGCCGAAATGACCGGTATCTTTGCCGTAACGCTCGCGG        <  SRR4302537.235688/94‑1 (MQ=60)
                                                                                      TCCGCCGCGCCAAACAACGCGGCAAAAACCTGCGGATCATCAATGGCGTCAATCATGCCGAAATGACCGGTATCTTTGCCGTAACGCTCGCGGT       <  SRR4302537.3336225/94‑1 (MQ=60)
                                                                                      TCCGCCGCGCCAAACAACGCGGCAAAAACCTGCGGATCATCAATGGCGTCAATCATGCCGAAATGACCGGTATCTTTGCCGTAACGCTCGCGGT       <  SRR4302537.217027/94‑1 (MQ=60)
                                                                                      TCCGCCGCGCCAAACAACGCGGCAAAAACCTGCGGATCATCAATGGCGTCAATCATGCCGAAATGACCGGTATCTTTGCCGTAACGCTCGCGGT       >  SRR4302537.2133222/1‑94 (MQ=60)
                                                                                       CCGCCGCGCCAAACAACGCGGCAAAAACCTGCGGATCATCAATGGCGTCAATCATGCCGAAATGACCGGTATCTTTGCCGTAACGCGCGCGGTG      >  SRR4302537.172721/1‑94 (MQ=60)
                                                                                        CGCCGCGCCAAACAACGCGGCAAAAACCTGCGGATCATCAATGGCGTCAATCATGCCGAAATGACCGGTATCTTTGCCGTAACGCTCGCGGTGC     >  SRR4302537.2507255/1‑94 (MQ=60)
                                                                                        CGCCGCGCCAAACAACGCGGCAAAAACCTGCGGATCATCAATGGCGTCAATCATGCCGAAATGACCGGTATCTTTGCCGTAACGCTCGCGGTGC     >  SRR4302537.2538546/1‑94 (MQ=60)
                                                                                         GCCGCGCCAAACAACGCGGCAAAAACCTGCGGATCATCAATGGCGTCAATCATGCCGAAATGACCGGTATCTTTGCCGTAACGCTCGCGGTGCA    <  SRR4302537.5727600/94‑1 (MQ=60)
                                                                                          CCGCGCCAAACAACGCGGCAAAAACCTGCGGATCATCAATGGCGTCAATCATGCCGAAATGACCGGTATCTTTGCCGTAACGCTCGCGGTGCAA   <  SRR4302537.4081277/94‑1 (MQ=60)
                                                                                          CCGCGCCAAACAACGCGGCAAAAACCTGCGGATCATCAATGGCGTCAATCATGCCGAAATGACCGGTATCTTTGCCGTAACGCTCGCGGTGCAA   <  SRR4302537.1129777/94‑1 (MQ=60)
                                                                                           CGCGCCAAACAACGCGGCAAAAACCTGCGGATCATCAATGGCGTCAATCATGCCGAAATGACCGGTATCTTTGCCGTAACGCTCGCGGTGCAAG  <  SRR4302537.2670198/94‑1 (MQ=60)
                                                                                           CGCGCCAAACAACGCGGCAAAAACCTGCGGATCATCAATGGCGTCAATCATGCCGAAATGACCGGTATCTTTGCCGTAACGCTCGCGGTGCAAG  <  SRR4302537.3089401/94‑1 (MQ=60)
                                                                                           CGCGCCAAACAACGCGGCAAAAACCTGCGGATCATCAATGGCGTCAATCATGCCGAAATGACCGGTATCTTTGCCGTAACGCTCGCGGTGCAAG  >  SRR4302537.3039890/1‑94 (MQ=60)
                                                                                           |                                                                                             
CTTCAATCAGTAATCCGCTTTCCTGATTGATATTCAGGCTGAAAGGACCGCTGATCTTACTTGCACCTTGTGACTTCAACCACGCTTCCGCTGCGCCAAACAACGCGGCAAAAACCTGCGGATCATCAATGGCGTCAATCATGCCGAAATGACCGGTATCTTTGCCGTAACGCTCGCGGTGCAAG  >  NC_000913/3127774‑3127958

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 2 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑