Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A1 F1 I2 R1	882	254.4	12957588	98.0%	12698436	93.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,203,801	T→C	T33A (ACG→GCG)	ygiP ←	putative transcriptional regulator LYSR‑type

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,203,801	0	T	C	96.3%	764.8 / 14.2	241	T33A (ACG→GCG)	ygiP	putative transcriptional regulator LYSR‑type
Reads supporting (aligned to +/- strand): ref base T (6/3); new base C (125/107); total (131/110)
Fisher's exact test for biased strand distribution p-value = 5.15e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.36e-01

Only 100 of 252 total aligned reads displayed.

TCAGCGCCACGCCGCGAGCGGAGCGGTTCAGCAGCGTTGTTGCCAGCGTATTTTCAAGGATTTGGATCCGCTTGGTGACAAATGCAGGCGTCTGCCCAAGCGTCGCCGCTGCGGCGCTAAAACTCCCGCTGTGAACGATTTCTACCAGTACCTGCAAGTCTTTGGCTAAAGGCCAGCTAT  >  NC_000913/3203711‑3203890

   gcgcCACGCCGCGAGCGGAGCGGTTCAGCAGCGTTGTTGCCAGCGTATTTTCAAGGATTTGGATCCGCTTGGTGACAAATGCAGGCGCCTGcc                                                                                      >  1:5978976/1‑93 (MQ=255)

     gcCACGCCGCGAGCGGAGCGGTTCAGCAGCGTTGTTGCCAGCGTATTTTCAAGGATTTGGATCCGCTTGGTGACAAATGCAGGCGCCTGCCCa                                                                                    >  1:2427248/1‑93 (MQ=255)

      ccACGCCGCGAGCGGAGCGGTTCAGCAGCGTTGTTGCCAGCGTATTTTCAAGGATTTGGATCCGCTTGGTGACAAATGCAGGCGCCTGCCCaa                                                                                   <  2:77190/93‑1 (MQ=255)

       cACGCCGCGAGCGGAGCGGTTCAGCAGCGTTGTTGCCAGCGTATTTTCAAGGATTTGGATCCGCTTGGTGACAAATGCAGGCGCCTGCCCAAg                                                                                  <  2:2616093/93‑1 (MQ=255)

        aCGCCGCGAGCGGAGCGGTTCAGCAGCGTTGTTGCCAGCGTATTTTCAAGGATTTGGATCCGCTTGGTGACAAATGCAGGCGCCTGCCCAAGc                                                                                 <  1:348983/93‑1 (MQ=255)

         cgccgcGAGCGGAGCGGTTCAGCAGCGTTGTTGCCAGCGTATTTTCAAGGATTTGGATCCGCTTGGTTACAAATGCAGGCGCCTGCCCAAGCg                                                                                <  2:1788953/93‑1 (MQ=255)

         cgccgcGAGCGGAGCGGTTCAGCAGCGTTGTTGCCAGCGTATTTTCAAGGATTTGGATCCGCTTGGTGACAAATGCAGGCGCCTGCCCAAGCg                                                                                <  1:3871136/93‑1 (MQ=255)

          gccgcGAGCGGAGCGGTTCAGCAGCGTTGTTGCCAGCGTATTTTCAAGGATTTGGATCCGCTTGGTGACAAATGCAGGCGCCTGCCCAAGCGt                                                                               >  2:4180439/1‑93 (MQ=255)

           gcgcgAGCGGAGCGGTTCAGCAGCGTTGTTGCCAGCGTATTTTCAAGGATTTGGATCCGCTTGGTGACAAATGCAGGCGCCTGCCCAAGCGTc                                                                              <  1:3950803/92‑1 (MQ=255)

            cgcgAGCGGAGCGGTTCAGCAGCGTTGTTGCCAGCGTATTTTCAAGGATTTGGATCCGCTTGGTGACAAATGCAGGCGCCTGCCCAAGCGTcg                                                                             >  2:6132358/1‑93 (MQ=255)

             gcgAGCGGAGCGGTTCAGCAGCGTTGTTGCCAGCGTATTTTCAAGGATTTGGATCCGCTTGGTGACAAATGCAGGCGCCTGCCCAAGCGTcgc                                                                            <  1:3321070/93‑1 (MQ=255)

                 gcggagcggTTCAGCAGCGTTGTTGCCAGCGTATTTTCAAGGATTTGGATCCGCTTGGTGACAAATGCAGGCGCCTGCCCAAGCGTCGCCGCt                                                                        <  1:2712952/93‑1 (MQ=255)

                   ggagcggTTCAGCAGCGTTGTTGCCAGCGTATTTTCAAGGATTTGGATCCGCTTGGTGACAAATGCAGGCGCCTGCCCAAGCGTCGCCGCTgc                                                                      >  2:2418379/1‑93 (MQ=255)

                    gagcggTTCAGCAGCGTTGTTGCCAGCGTATTTTCAAGGATTTGGATCCGCTTGGTGACAAATGCAGGCGTCTGCCCAAGCGTCGCCGCTgcg                                                                     >  1:2739757/1‑93 (MQ=255)

                    gagcggTTCAGCAGCGTTGTTGCCAGCGTATTTTCAAGGATTTGGATCCGCTTGGTGACAAATGCAGGCGCCTGCCCAAGCGTCGCCGCTgcg                                                                     <  2:4467186/93‑1 (MQ=255)

                    gagcggTTCAGCAGCGTTGTTGCCAGCGTATTTTCAAGGATTTGGATCCGCTTGGTGACAAATGCAGGCGCCTGCCCAAGCGTCGCCGCTgcg                                                                     <  1:1959841/93‑1 (MQ=255)

                    gagcggTTCAGCAGCGTTGTTGCCAGCGTATTTTCAAGGATTTGGATCCGCTTGGTGACAAATGCAGGCGCCTGCCCAAGCGTCGCCGCTgcg                                                                     >  2:4969773/1‑93 (MQ=255)

                    gagcggTTCAGCAGCGTTGTTGCCAGCGTATTTTCAAGGATTTGGATCCGCTTGGTGACAAATGCAGGCGCCTGCCCAAGCGTCGCCGCTgcg                                                                     >  1:857151/1‑93 (MQ=255)

                     agcggTTCAGCAGCGTTGTTGCCAGCGTATTTTCAAGGATTTGGATCCGCTTGGTGACAAATGCAGGCGCCTGCCCAAGCGTCGCCGCTgcgg                                                                    >  1:5764924/1‑93 (MQ=255)

                     agcggTTCAGCAGCGTTGTTGCCAGCGTATTTTCAAGGATTTGGATCCGCTTGGTGACAAATGCAGGCGCCTGCCCAAGCGTCGCCGCTgcgg                                                                    <  1:1106194/93‑1 (MQ=255)

                      gcggTTCAGCAGCGTTGTTGCCAGCGTATTTTCAAGGATTTGGATCCGCTTGGTGACAAATGCAGGCGCCTGCCCAAGCGTCGCCGCTgcggc                                                                   <  2:4543372/93‑1 (MQ=255)

                      gcggTTCAGCAGCGTTGTTGCCAGCGTATTTTCAAGGATTTGGATCCGCTTGGTGACAAATGCAGGCGCCTGCCCAAGCGTCGCCGCTgcggc                                                                   <  1:1683249/93‑1 (MQ=255)

                        ggTTCAGCAGCGTTGTTGCCAGCGTATTTTCAAGGATTTGGATCCGCTTGGTGACAAATGCAGGCGCCTGCCCAAGCGTCGCTGCTGCGgcgc                                                                 <  1:4652816/93‑1 (MQ=255)

                        ggTTCAGCAGCGTTGTTGCCAGCGTATTTTCAAGGATTTGGATCCGCTTGGTGACAAATGCAGGCGCCTGCCCAAGCGTCGCCGCTGCGgcgc                                                                 >  2:3652113/1‑93 (MQ=255)

                        ggTTCAGCAGCGTTGTTGCCAGCGTATTTTCAAGGATTTGGATCCGCTTGGTGACAAATGCAGGCGCCTGCCCAAGCGTCGCCGCTGCGgcgc                                                                 >  1:214200/1‑93 (MQ=255)

                        ggTTCAGCAGCGTTGTTGCCAGCGTATTTTCAAGGATTTGGATCCGCTTGGTGACAAATGCAGGCGCCTGCCCAAGCGTCGCCGCTGCGgcgc                                                                 >  1:2837928/1‑93 (MQ=255)

                         gTTCAGCAGCGTTGTTGCCAGCGTATTTTCAAGGATTTGGATCCGCTTGGTGACAAATGCAGGCGCCTGCCCAAGCGTCGCCGCTGCGGCGCt                                                                >  1:4336529/1‑93 (MQ=255)

                         gTTCAGCAGCGTTGTTGCCAGCGTATTTTCAAGGATTTGGATCCGCTTGGTGACAAATGCAGGCGCCTGCCCAAGCGTCGCCGCTGCGGCGCt                                                                >  1:4129619/1‑93 (MQ=255)

                             agcagcGTTGTTGCCAGCGTATTTTCAAGGATTTGGATCCGCTTGGTGACAAATGCAGGCGTCTGCCCAAGCGTCGCCGCTGCGGCGCTaaaa                                                            <  1:4286213/93‑1 (MQ=255)

                             agcagcGTTGTTGCCAGCGTATTTTCAAGGATTTGGATCCGCTTGGTGACAAATGCAGGCGCCTGCCCAAGCGTCGCCGCTGCGGCGCTaaaa                                                            >  1:2257514/1‑93 (MQ=255)

                              gcagcGTTGTTGCCAGCGTATTTTCAAGGATTTGGATCCGCTTGGTGACAAATGCAGGCGCCTGCCCAAGCGTCGCCGCTGCGGCGCTAAAAc                                                           <  2:1823060/93‑1 (MQ=255)

                                agcGTTGTTGCCAGCGTATTTTCAAGGATTTGGATCCGCTTGGTGACAAATGAAGGCGCCTGCCCAAGCGTCGCCGCTGCGGCGCTAAAACTc                                                         <  2:346513/93‑1 (MQ=255)

                                 gcGTTGTTGCCAGCGTATTTTCAAGGATTTGGATCCGCTTGGTGACAAATGCAGGCGCCTGCCCAAGCGTCGCCGCTGCGGCGCTAAAACTcc                                                        <  2:595926/93‑1 (MQ=255)

                                 gcGTTGTTGCCAGCGTATTTTCAAGGATTTGGATCCGCTTGGTGACAAATGCAGGCGCCTGCCCAAGCGTCGCCGCTGCGGCGCTAAAACTcc                                                        >  2:5316082/1‑93 (MQ=255)

                                  cGTTGTTGCCAGCGTATTTTCAAGGATTTGGATCCGCTTGGTGACAAATGCAGGCGCCTGCCCAAGCGTCGCCGCTGCGGCGCTAAAACTccc                                                       <  2:2963162/93‑1 (MQ=255)

                                    ttgttgCCAGCGTATTTTCAAGGATTTGGATCCGCTTGGTGACAAATGCAGGCGCCTGCCCAAGCGTCGCCGCTGCGGCGCTAAAACTCCCGc                                                     >  1:844951/1‑93 (MQ=255)

                                    ttgttgCCAGCGTATTTTCAAGGATTTGGATCCGCTTGGTGACAAATGCAGGCGCCTGCCCAAGCGTCGCCGCTGCGGCGCTAAAACTCCCGc                                                     >  1:197561/1‑93 (MQ=255)

                                    ttgttgCCAGCGTATTTTCAAGGATTTGGATCCGCTTGGTGACAAATGCAGGCGCCTGCCCAAGCGTCGCCGCTGCGGCGCTAAAACTCCCGc                                                     >  2:2100236/1‑93 (MQ=255)

                                    ttgttgCCAGCGTATTTTCAAGGATTTGGATCCGCTTGGTGACAAATGCAGGCGCCTGCCCAAGCGTCGCCGCTGCGGCGCTAAAACTCCCGc                                                     >  1:1482232/1‑93 (MQ=255)

                                      gttgCCAGCGTATTTTCAAGGATTTGGATCCGCTTGGTGACAAATGCAGGCGCCTGCCCAAGCGTCGCCGCTGCGGCGCTAAAACTCCCGCtg                                                   <  1:5266648/93‑1 (MQ=255)

                                          ccAGCGTATTTTCAAGGATTTGGATCCGCTTGGTGACAAATGCAGGCGCCTGCCCAAGCGTCGCCGCTGCGGCGCTAAAGCTCCCGCTGTGaa                                               <  2:3829419/93‑1 (MQ=255)

                                          ccAGCGTATTTTCAAGGATTTGGATCCGCTTGGTGACAAATGCAGGCGCCTGCCCAAGCGTCGCCGCTGCGGCGCTAAAACTCCCGCTGTGaa                                               <  2:4077723/93‑1 (MQ=255)

                                          ccAGCGTATTTTCAAGGATTTGGATCCGCTTGGTGACAAATGCAGGCGCCTGCCCAAGCGTCGCCGCTGCGGCGCTAAAACTCCCGCTGTGaa                                               <  1:1834464/93‑1 (MQ=255)

                                          ccAGCGTATTTTCAAGGATTTGGATCCGCTTGGTGACAAATGCAGGCGCCTGCCCAAGCGTCGCCGCTGCGGCGCTAAAACTCCCGCTGTGaa                                               <  1:5285428/93‑1 (MQ=255)

                                          ccAGCGTATTTTCAAGGATTTGGATCCGCTTGGTGACAAATGCAGGCGCCTGCCCAAGCGTCGCCGCTGCGGCGCTAAAACTCCCGCTGTGaa                                               <  2:3186333/93‑1 (MQ=255)

                                           cAGCGTATTTTCAAGGATTTGGATCCGCTTGGTGACAAATGCAGGCGCCTGCCCAAGCGTCGCCGCTGCGGCGCTAAAACTCCCGCTGTGAAc                                              <  1:5643757/93‑1 (MQ=255)

                                           cAGCGTATTTTCAAGGATTTGGATCCGCTTGGTGACAAATGCAGGCGCCTGCCCAAGCGTCGCCGCTGCGGCGCTAAAACTCCCGCTGTGAAc                                              <  1:6086820/93‑1 (MQ=255)

                                           cAGCGTATTTTCAAGGATTTGGATCCGCTTGGTGACAAATGCAGGCGCCTGCCCAAGCGTCGCCGCTGCGGCGCTAAAACTCCCGCTGTGAAc                                              >  2:470701/1‑93 (MQ=255)

                                            aGCGTATTTTCAAGGATTTGGATCCGCTTGGTGACAAATGCAGGCGTCTGCCCAAGCGTCGCCGCTGCGGCGCTAAAACTCCCGCTGTGAACg                                             >  2:5868758/1‑93 (MQ=255)

                                            aGCGTATTTTCAAGGATTTGGATCCGCTTGGTGACAAATGCAGGCGCCTGCCCAAGCGTCGCCGCTGCGGCGCTAAAACTCCCGCTGTGAACg                                             >  1:3369592/1‑93 (MQ=255)

                                            aGCGTATTTTCAAGGATTTGGATCCGCTTGGTGACAAATGCAGGCGCCTGCCCAAGCGTCGCCGCTGCGGCGCTAAAACTCCCGCTGTGAACg                                             >  1:5378953/1‑93 (MQ=255)

                                             gCGTATTTTCAAGGATTTGGATCCGCTTGGTGACAAATGCAGGCGCCTGCCCAAGCGTCGCCGCTGCGGCGCTAAAACTCCCGCTGTGAACGa                                            >  2:5405250/1‑93 (MQ=255)

                                                  ttttCAAGGATTTGGATCCGCTTGGTGACAAATGCAGGCGCCTGCCCAAGCGTCGCCGCTGCGGCGCTAAAACTCCCGCTGTGAACGATTTCt                                       <  2:5125778/93‑1 (MQ=255)

                                                    ttCAAGGATTTGGATCCGCTTGGTGACAAATGCAGGCGCCTGCCCAAGCGTCGCCGCTGCGGCGCTAAAACTCCCGCTGTGAACGATTTCTAc                                     >  2:694722/1‑93 (MQ=255)

                                                       aaGGATTTGGATCCGCTTGGTGACAAATGCAGGCGCCTGCCCAAGCGTCGCCGCTGCGGCGCTAAAACTCCCGCTGTGAACGATTTCTACCAg                                  >  1:4388083/1‑93 (MQ=255)

                                                        aGGATTTGGATCCGCTTGGTGACAAATGCAGGCGCCTGCCCAGGCGTCGCCGCTGCGGCGCTAAAACTCCCGCTGTGAACGATTTCTACCAGt                                 >  1:3457990/1‑93 (MQ=255)

                                                        aGGATTTGGATCCGCTTGGTGACAAATGCAGGCGCCTGCCCAAGCGTCGCCGCTGCGGCGCTAAAACTCCCGCTGTGAACGATTTCTACCAGt                                 <  2:3505513/93‑1 (MQ=255)

                                                          gATTTGGATCCGCTTGGTGACAAATGCAGGCGCCTGCCCAAGCGTCGCCGCTGCGGCGCTAAAACTCCCGCTGTGAACGATTTCTACCAGTAc                               >  2:1586892/1‑93 (MQ=255)

                                                           aTTTGGATCCGCTTGGTGACAAATGCAGGCGCCTGCCCAAGCGTCGCCGCTGCGGCGCTAAAACTCCCGCTGTGAACGATTTCTACCAGTAcc                              >  1:6119719/1‑93 (MQ=255)

                                                           aTTTGGATCCGCTTGGTGACAAATGCAGGCGCCTGCCCAAGCGTCGCCGCTGCGGCGCTAAAACTCCCGCTGTGAACGATTTCTACCAGTAcc                              >  1:2062762/1‑93 (MQ=255)

                                                           aTTTGGATCCGCTTGGTGACAAATGCAGGCGCCTGCCCAAGCGTCGCCGCTGCGGCGCTAAAACTCCCGCTGTGAACGATTTCTACCAGTAcc                              <  2:3132433/93‑1 (MQ=255)

                                                            tttGGATCCGCTTGGTGACAAATGCAGGCGCCTGCCCAAGCGTCGCCGCTGCGGCGCTAAAACTCCCGCTGTGAACGATTTCTACCAGTACCt                             <  2:5663613/93‑1 (MQ=255)

                                                            tttGGATCCGCTTGGTGACAAATGCAGGCGCCTGCCCAAGCGTCGCCGCTGCGGCGCTAAAACTCCCGCTGTGAACGATTTCTACCAGTACCt                             <  1:6224659/93‑1 (MQ=255)

                                                             ttGGATCCGCTTGGTGACAAATGCAGGCGCCTGCCCAAGCGTCGCCGCTGCGGCGCTAAAACTCCCGCTGTGAACGATTTCTACCAGTACCTg                            <  2:309509/93‑1 (MQ=255)

                                                              tGGATCCGCTTGGTGACAAATGCAGGCGCCTGCCCAAGCGTCGCCGCTGCGGCGCTAAAACTCCCGCTGTGAACGATTTCTACCAGTACCTGc                           <  1:4535029/93‑1 (MQ=255)

                                                               ggATCCGCTTGGTGACAAATGCAGGCGCCTGCCCAAGCGTCGCCGCTGCGGCGCTAAAACTCCCGCTGTGAACGATTTCTACCAGTACCTGCa                          >  2:1138547/1‑93 (MQ=255)

                                                                gATCCGCTTGGTGACAAATGCAGGCGCCTGCCCAAGCGTCGCCGCTGCGGCGCTAAAACTCCCGCTGTGAACGATTTCTACCAGTACCTGCaa                         <  1:1189608/93‑1 (MQ=255)

                                                                 aTCCGCTTGGTGACAAATGCAGGCGCCTGCCCAAGCGTCGCCGCTGCGGCGCTAAAACTCCCGCTGTGAACGATTTCTACCAGTACCTGCAAg                        >  2:1460947/1‑93 (MQ=255)

                                                                 aTCCGCTTGGTGACAAATGCAGGCGCCTGCCCAAGCGTCGCCGCTGCGGCGCTAAAACTCCAGCTGTGAACGATTTCTACCAGTACCTGCAAg                        <  1:4498592/93‑1 (MQ=255)

                                                                  tCCGCTTGGTGCCAAATGCAGGTGCCTGCCCAAGCGTCGCCGCTGCGGCGCTAAAACTCCCGCTGTGAACGATTTCTACCAGTAACAGCAAGt                       >  2:1661474/1‑93 (MQ=255)

                                                                  tCCGCTTGGTGACAAATGCAGGCGCCTGCCCAAGCGTCGCCGCTGCGGCGCTAAAACTCCCGCTGTGAACGATTTCTACCAGTACCTGCAAGt                       >  2:3237263/1‑93 (MQ=255)

                                                                   ccGCTTGGTGACAAATGCAGGCGCCTGCCCAAGCGTCGCCGCTGCGGCGCTAAAACTCCCGCTGTGAACGATTTCTACCAGTACCTGCAAGTc                      >  1:2528383/1‑93 (MQ=255)

                                                                   ccGCTTGGTGACAAATGCAGGCGCCTGCCCAAGCGTCGCCGCTGCGGCGCTAAAACTCCCGCTGTGAACGATTTCTACCAGTACCTGCAAGTc                      >  2:100143/1‑93 (MQ=255)

                                                                    cGCTTGGTGACAAATGCAGGCGCCTGCCCAAGCGTCGCCGCTGCGGCGCTAAAACTCCCGCTGTGAACGATTTCTACCAGTACCTGCAAGTCt                     <  1:3106497/93‑1 (MQ=255)

                                                                     gCTTGGTGACAAATGCAGGCGCCTGCCCAAGCGTCGCCGCTGCGGCGCTAAAACTCCCGCTGTGAACGATTTCTACCAGTACCTGCAAGTCtt                    >  1:3560677/1‑93 (MQ=255)

                                                                      cTTGGTGACAAATGCAGGCGCCTGCCCAAGCGTCGCCGCTGCGGCGCTAAAACTCCCGCTGTGAACGATTTCTACCAGTACCTGCAAGTCttt                   <  2:569272/93‑1 (MQ=255)

                                                                      cTTGGTGACAAATGCAGGCGCCTGCCCAAGCGTCGCCGCTGCGGCGCTAAAACTCCCGCTGTGAACGATTTCTACCAGTACCTGCAAGTCttt                   >  2:5253714/1‑93 (MQ=255)

                                                                      cTTGGTGACAAATGCAGGCGCCTACCCAAGCGTCGCCGCTGCGGCGCTAAAACTCCCGCTGTGAACGATTTCTACCAGTACCTGCAAGTCttt                   <  1:2103103/93‑1 (MQ=255)

                                                                       ttGGTGACAAATGCAGGCGTCTGCCCAAGCGTCGCCGCTGCGGCGCTAAAACTCCCGCTGTGAACGATTTCTACCAGTACCTGCAAGTCTTTg                  >  2:6315550/1‑93 (MQ=255)

                                                                        tGGTGACAAATGCAGGCGCCTGCCCAAGCGTCGCCGCTGCGGCGCTAAAACTCCCGCTGTGAACGATTTCTACCAGTACCTGCAAGTCTTTgg                 <  1:5480173/93‑1 (MQ=255)

                                                                         ggTGACAAATGCAGGCGCCTGCCCAAGCGTCGCCGCTGCGGCGCTAAAACTCCCGCTGTGAACGATTTCTACCAGTACCTGCAAGTCTTTGGc                >  1:5021809/1‑93 (MQ=255)

                                                                          gTGACAAATGCAGGCGCCTGCCCAAGCGTCGCCGCTGCGGCGCTAAAACTCCCGCTGTGAACGATTTCTACCAGTACCTGCAAGTCTTTGGCt               >  2:4335122/1‑93 (MQ=255)

                                                                          gTGACAAATGCAGGCGCCTGCCCAAGCGTCGCCGCTGCGGCGCTAAAAATCCCGCTGTGAACGATTTCTACCAGTACCTGCAAGTCTTTGGCt               >  1:1771342/1‑93 (MQ=255)

                                                                            gACAAATGCAGGCGCCTGCCCAAGCGTCGCCGCTGCGGCGCTAAAACTCCCGCTGTGAACGATTTCTACCAGTACCTGCAAGTCTTTGGCTaa             >  2:2358016/1‑93 (MQ=255)

                                                                            gACAAATGCAGGCGCCTGCCCAAGCGTCGCCGCTGCGGCGCTAAAACTCCCGCTGTGAACGATTTCTACCAGTACCTGCAAGTCTTTGGCTaa             <  2:4870628/93‑1 (MQ=255)

                                                                             aCAAATGCAGGCGCCTGCCCAAGCGTCGCCGCTGCGGCGCTAAAACTCCCGCTGTGAACGATTTCTACCAGTACCTGCAAGTCTTTGGCTaaa            <  1:3652987/93‑1 (MQ=255)

                                                                              cAAATGCAGGCGCCTGCCCAAGCGTCGCCGCTGCGGCGCTAAAACTCCCGCTGTGAACGATTTCTACCAGTACCTGCAAGTCTTTGGCTAAAg           <  1:6335706/93‑1 (MQ=255)

                                                                              cAAATGCAGGCGCCTGCCCAAGCGTCGCCGCTGCGGCGCTAAAACTCCCGCTGTGAACGATTTCTACCAGTACCTGCAAGTCTTTGGCTAAAg           <  2:3694300/93‑1 (MQ=255)

                                                                              cAAATGCAGGCGCCTGCCCAAGCGTCGCCGCTGCGGCGCTAAAACTCCCGCTGTGAACGATTTCTACCAGTACCTGCAAGTCTTTGGCTAAAg           >  1:3036908/1‑93 (MQ=255)

                                                                              cAAATGCAGGCGCCTGCCCAAGCGTCGCCGCTGCGGCGCTAAAACTCCCGCTGTGAACGATTTCTACCAGTACCTGCAAGTCTTTGGCTAAAg           <  2:3404127/93‑1 (MQ=255)

                                                                              cAAATGCAGGCGCCTGCCCAAGCGTCGCCGCTGCGGCGCTAAAACTCCCGCTGTGAACGATTTCTACCAGTACCTGCAAGTCTTTGGCTAAAg           <  2:2499314/93‑1 (MQ=255)

                                                                               aaaTGCAGGCGCCTGCCCAAGCGTCGCCGCTGCGGCGCTAAAACTCCCGCTGTGAACGATTTCTACCAGTACCTGCAAGTCTTTGGCTAAAgg          <  1:3753229/93‑1 (MQ=255)

                                                                               aaaTGCAGGCGCCTGCCCAAGCGTCGCCGCTGCGGCGCTAAAACTCCCGCTGTGAACGATTTCTACCAGTACCTGCAAGTCTTTGGCTAAAgg          <  2:1952951/93‑1 (MQ=255)

                                                                                aaTGCAGGCGCCTGCCCAAGCGTCGCCGCTGCGGCGCTAAAACTCCCGCTGTGAACGATTTCTACCAGTACCTGCAAGTCTTTGGCTAAAGGc         <  2:6188160/93‑1 (MQ=255)

                                                                                  tGCAGGCGCCTGCCCAAGCGTCGCCGCTGCGGCGCTAAAACTCCCGCTGTGAACGATTTCTACCAGTACCTGCAAGTCTTTGGCTAAAGGCCa       <  2:4653233/93‑1 (MQ=255)

                                                                                  tGCAGGCGCCTGCCCAAGCGTCGCCGCTGCGGCGCTAAAACTCCCGCTGTGAACGATTTCTACCAGTACCTGCAAGTCTTTGGCTAAAGGCCa       >  1:3919510/1‑93 (MQ=255)

                                                                                   gCAGGCGCCTGCCCAAGCGTCGCCGCTGCGGCGCTAAAACTCCCGCTGTGAACGATTTCTACCAGTACCTGCAAGTCTTTGGCTAAAGGCCAg      <  1:4764600/93‑1 (MQ=255)

                                                                                    cAGGCGCCTGCCCAAGCGTCGCCGCTGCGGCGCTAAAACTCCCGCTGTGAACGATTTCTACCAGTACCTGCAAGTCTTTGGCTAAAGGCCAGc     >  1:8340/1‑93 (MQ=255)

                                                                                       gcgcCTGCCCAAGCGTCGCCGCTGCGGCGCTAAAACTCCCGCTGTGAACGATTTCTACCAGTACCTGCAAGTCTTTGGCTAAAGGCCAGCTAt  <  1:2912668/93‑1 (MQ=255)

                                                                                       gcgcCTGCCCAAGCGTCGCCGCTGCGGCGCTAAAACTCCCGCTGTGAACGATTTCTACCAGTACCTGCAAGTCTTTGGCTAAAGGCCAGCTAt  <  2:1643276/93‑1 (MQ=255)

TCAGCGCCACGCCGCGAGCGGAGCGGTTCAGCAGCGTTGTTGCCAGCGTATTTTCAAGGATTTGGATCCGCTTGGTGACAAATGCAGGCGTCTGCCCAAGCGTCGCCGCTGCGGCGCTAAAACTCCCGCTGTGAACGATTTCTACCAGTACCTGCAAGTCTTTGGCTAAAGGCCAGCTAT  >  NC_000913/3203711‑3203890

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 22 ≤` `ATCG/ATCG` `< 30 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

BRESEQ :: bam2aln output

GTCAGCGCCACGCCGCGAGCGGAGCGGTTCAGCAGCGTTGTTGCCAGCGTATTTTCAAGGATTTGGATCCGCTTGGTGACAAATGCAGGCGTCTGCCCAAGCGTCGCCGCTGCGGCGCTAAAACTCCCGCTGTGAACGATTTCTACCAGTACCTGCAAGTCTTTGGCTAAAGGCCAGCTATTCAG  >  NC_000913/3203710‑3203894

GTCAGCGCCACGCCGCGAGCGGAGCGGTTCAGCAGCGTTGTTGCCAGCGTATTTTCAAGGATTTGGATCCGCTTGGTGACAAATGCAGGCGCCT                                                                                             <  SRR4302537.3144397/94‑1 (MQ=60)

 TCAGCGCCACGCCGCGAGCGGAGCGGTTCAGCAGCGTTGTTGCCAGCGTATTTTCAAGGATTTGGATCCGCTTGGTGACAAATGCAGGCGCCTG                                                                                            <  SRR4302537.3955050/94‑1 (MQ=60)

   AGCGCCACGCCGCGAGCGGAGCGGTTCAGCAGCGTTGTTGCCAGCGTATTTTCAAGGATTTGGATCCGCTTGGTGACAAATGCAGGCGCCTGCC                                                                                          <  SRR4302537.6080110/94‑1 (MQ=60)

   AGCGCCACGCCGCGAGCGGAGCGGTTCAGCAGCGTTGTTGCCAGCGTATTTTCAAGGATTTGGATCCGCTTGGTGACAAATGCAGGCGCCTGCC                                                                                          >  SRR4302537.6020016/1‑94 (MQ=60)

   AGCGCCACGCCGCGAGCGGAGCGGTTCAGCAGCGTTGTTGCCAGCGTATTTTCAAGGATTTGGATCCGCTTGGTGACAAATGCAGGCGCCTGCC                                                                                          >  SRR4302537.5334116/1‑94 (MQ=60)

   AGCGCCACGCCGCGAGCGGAGCGGTTCAGCAGCGTTGTTGCCAGCGTATTTTCAAGGATTTGGATCCGCTTGGTGACAAATGCAGGCGCCTGCC                                                                                          >  SRR4302537.4416997/1‑94 (MQ=60)

    GCGCCACGCCGCGAGCGGAGCGGTTCAGCAGCGTTGTTGCCAGCGTATTTTCAAGGATTTGGATCCGCTTGGTGACAAATGCAGGCGCCTGCCC                                                                                         <  SRR4302537.1738029/94‑1 (MQ=60)

     CGCCACGCCGCGAGCGGAGCGGTTCAGCAGCGTTGTTGCCAGCGTATTTTCAAGGATTTGGATCCGCTTGGTGACAAATGCAGGCGCCTGCCCA                                                                                        >  SRR4302537.3530318/1‑94 (MQ=60)

     CGCCACGCCGCGAGCGGAGCGGTTCAGCAGCGTTGTTGCCAGCGTATTTTCAAGGATTTGGATCCGCTTGGTGACAAATGCAGGCGCCTGCCCA                                                                                        >  SRR4302537.2444351/1‑94 (MQ=60)

      GCCACGCCGCGAGCGGAGCGGTTCAGCAGCGTTGTTGCCAGCGTATTTTCAAGGATTTGGATCCGCTTGGTGACAAATGCAGGCGCCTGCCCAA                                                                                       <  SRR4302537.1252659/94‑1 (MQ=60)

      GCCACGCCGCGAGCGGAGCGGTTCAGCAGCGTTGTTGCCAGCGTATTTTCAAGGATTTGGATCCGCTTGGTGACAAATGCAGGCGCCTGCCCAA                                                                                       >  SRR4302537.5947353/1‑94 (MQ=60)

       CCACGCCGCGAGCGGAGCGGTTCAGCAGCGTTGTTGCCAGCGTATTTTCAAGGATTTGGATCCGCTTGGTGACAAATGCAGGCGCCTGCCCAAG                                                                                      <  SRR4302537.3730014/94‑1 (MQ=60)

       CCACGCCGCGAGCGGAGCGGTTCAGCAGCGTTGTTGCCAGCGTATTTTCAAGGATTTGGATCCGCTTGGTGACAAATGCAGGCGCCTGCCCAAG                                                                                      <  SRR4302537.2443649/94‑1 (MQ=60)

       CCACGCCGCGAGCGGAGCGGTTCAGCAGCGTTGTTGCCAGCGTATTTTCAAGGATTTGGATCCGCTTGGTGACAAATGCAGGCGCCTGCCCAAG                                                                                      <  SRR4302537.4512282/94‑1 (MQ=60)

        CACGCCGCGAGCGGAGCGGTTCAGCAGCGTTGTTGCCAGCGTATTTTCAAGGATTTGGATCCGCTTGGTGACAAATGCAGGCGCCTGCCCAAGC                                                                                     >  SRR4302537.3205035/1‑94 (MQ=60)

         ACGCCGCGAGCGGAGCGGTTCAGCAGCGTTGTTGCCAGCGTATTTTCAAGGATTTGGATCCGCTTGGTGACAAATGCAGGCGCCTGCCCAAGCG                                                                                    <  SRR4302537.351407/94‑1 (MQ=60)

          CGCCGCGAGCGGAGCGGTTCAGCAGCGTTGTTGCCAGCGTATTTTCAAGGATTTGGATCCGCTTGGTGACAAATGCAGGCGCCTGCCCAAGCGT                                                                                   <  SRR4302537.3898226/94‑1 (MQ=60)

           GCCGCGAGCGGAGCGGTTCAGCAGCGTTGTTGCCAGCGTATTTTCAAGGATTTGGATCCGCTTGGTGACAAATGCAGGCGCCTGCCCAAGCGTC                                                                                  <  SRR4302537.3958618/94‑1 (MQ=60)

           GCCGCGAGCGGAGCGGTTCAGCAGCGTTGTTGCCAGCGTATTTTCAAGGACTTGGATCCGCTTGGTGACAAATGCAGGCGCCTGCCCAAGCGTC                                                                                  <  SRR4302537.3484968/94‑1 (MQ=60)

            GCGCGAGCGGAGCGGTTCAGCAGCGTTGTTGCCAGCGTATTTTCAAGGATTTGGATCCGCTTGGTGACAAATGCAGGCGCCTGCCCAAGCGTCG                                                                                 <  SRR4302537.3978434/94‑1 (MQ=60)

            CCGCGAGCGGAGCGGTTCAGCAGCGTTGTTGCCAGCGTATTTTCAAGGATTTGGATCCGCTTGGTGACAAATGCAGGCGCCTGCCCAAGCGTCG                                                                                 >  SRR4302537.2166032/1‑94 (MQ=60)

            CCGCGAGCGGAGCGGTTCAGCAGCGTTGTTGCCAGCGTATTTTCAAGGATTTGGATCCGCTTGGTGACAAATGCAGGCGCCTGCCCAAGCGTCG                                                                                 <  SRR4302537.2852116/94‑1 (MQ=60)

              GCGAGCGGAGCGGTTCAGCAGCGTTGTTGCCAGCGTATTTTCAAGGATTTGGATCCGCTTGGTGACAAATGCAGGCGCCTGCCCAAGCGTCGCC                                                                               <  SRR4302537.3344562/94‑1 (MQ=60)

              GCGAGCGGAGCGGTTCAGCAGCGTTGTTGCCAGCGTATTTTCAAGGATTTGGATCCGCTTGGTGACAAATGCAGGCGCCTGCCCAAGCGTCGCC                                                                               >  SRR4302537.1903735/1‑94 (MQ=60)

              GCGAGCGGAGCGGTTCAGCAGCGTTGTTGCCAGCGTATTTTCAAGGATTTGGATCCGCTTGGTGACAAATGCAGGCGCCTGCCCAAGCGTCGCC                                                                               <  SRR4302537.1889282/94‑1 (MQ=60)

                  GCGGAGCGGTTCAGCAGCGTTGTTGCCAGCGTATTTTCAAGGATTTGGATCCGCTTGGTGACAAATGCAGGCGCCTGCCCAAGCGTCGCCGCTG                                                                           <  SRR4302537.2731900/94‑1 (MQ=60)

                    GGAGCGGTTCAGCAGCGTTGTTGCCAGCGTATTTTCAAGGATTTGGATCCGCTTGGTGACAAATGCAGGCGTCTGCCCAAGCGTCGCCGCTGCG                                                                         >  SRR4302537.2758876/1‑94 (MQ=60)

                    GGAGCGGTTCAGCAGCGTTGTTGCCAGCGTATTTTCAAGGATTTGGATCCGCTTGGTGACAAATGCAGGCGCCTGCCCAAGCGTCGCCGCTGCG                                                                         >  SRR4302537.4167201/1‑94 (MQ=60)

                    GGAGCGGTTCAGCAGCGTTGTTGCCAGCGTATTTTCAAGGATTTGGATCCGCTTGGTGACAAATGCAGGCGCCTGCCCAAGCGTCGCCGCTGCG                                                                         >  SRR4302537.863034/1‑94 (MQ=60)

                    GGACCGGTTCAGCAGCGTTGTTCCCAGCGTATTTTCAAGGATTCGAATCCGCTCGGTGACAATTGCAGGCCCCTGCCCAAGCGTCGCCGCTGCG                                                                         <  SRR4302537.3839214/94‑1 (MQ=60)

                     GAGCGGTTCAGCAGCGTTGTTGCCAGCGTATTTTCAAGGATTTGGATCCGCTTGGTGACAAATGCAGGCGCCTGCCCAAGCGTCGCCGCTGCGG                                                                        >  SRR4302537.3464759/1‑94 (MQ=60)

                     GAGCGGTTCAGCAGCGTTGTTGCCAGCGTATTTTCAAGGATTTGGATCCGCTTGGTGACAAATGCAGGCGCCTGCCCAAGCGTCGCCGCTGCGG                                                                        <  SRR4302537.1973473/94‑1 (MQ=60)

                     GAGCGGTTCAGCAGCGTTGTTGCCAGCGTATTTTCAAGGATTTGGATCCGCTTGGTGACAAATGCAGGCGCCTGCCCAAGCGTCGCCGCTGCGG                                                                        >  SRR4302537.5804413/1‑94 (MQ=60)

                      AGCGGTTCAGCAGCGTTGTTGCCAGCGTATTTTCAAGGATTTGGATCCGCTTGGTGACAAATGCAGGCGCCTGCCCAAGCGTCGCCGCTGCGGC                                                                       >  SRR4302537.6322629/1‑94 (MQ=60)

                      AGCGGTTCAGCAGCGTTGTTGCCAGCGTATTTTCAAGGATTTGGATCCGCTTGGTGACAAATGCAGGCGCCTGCCCAAGCGTCGCCGCTGCGGC                                                                       <  SRR4302537.1113834/94‑1 (MQ=60)

                      AGCGGTTCAGCAGCGTTGTTGCCAGCGTATTTTCAAGGATTTGGATCCGCTTGGTGACAAATGCAGGCGCCTGCCCAAGCGTCGCCGCTGCGGC                                                                       <  SRR4302537.1113833/94‑1 (MQ=60)

                       GCGGTTCAGCAGCGTTGTTGCCAGCGTATTTTCAAGGATTTGGATCCGCTTGGTGACAAATGCAGGCGCCTGCCCAAGCGTCGCCGCTGCGGCG                                                                      <  SRR4302537.1695031/94‑1 (MQ=60)

                        CGGTTCAGCAGCGTTGTTGCCAGCGTATTTTCAAGGATTTGGATCCGCTTGGTGACAAATGCAGGCGCCTGCCCAAGCGTCGCCGCTGCGGCGC                                                                     >  SRR4302537.215726/1‑94 (MQ=60)

                        CGGTTCAGCAGCGTTGTTGCCAGCGTATTTTCAAGGATTTGGATCCGCTTGGTGACAAATGCAGGCGCCTGCCCAAGCGTCGCCGCTGCGGCGC                                                                     >  SRR4302537.2857784/1‑94 (MQ=60)

                         GGTTCAGCAGCGTTGTTGCCAGCGTATTTTCAAGGATTTGGATCCGCTTGGTGACAAATGCAGGCGCCTGCCCAAGCGTCGCTGCTGCGGCGCT                                                                    <  SRR4302537.4684991/94‑1 (MQ=60)

                         GGTTCAGCAGCGTTGTTGCCAGCGTATTTTCAAGGATTTGGATCCGCTTGGTGACAAATGCAGGCGCCTGCCCAAGCGTCGCCGCTGCGGCGCT                                                                    >  SRR4302537.4158441/1‑94 (MQ=60)

                         GGTTCAGCAGCGTTGTTGCCAGCGTATTTTCAAGGATTTGGATCCGCTTGGTGACAAATGCAGGCGCCTGCCCAAGCGTCGCCGCTGCGGCGCT                                                                    >  SRR4302537.4767761/1‑94 (MQ=60)

                         GGTTCAGCAGCGTTGTTGCCAGCGTATTTTCAAGGATTTGGATCCGCTTGGTGACAAATGCAGGCGCCTGCCCAAGCGTCGCCGCTGCGGCGCT                                                                    >  SRR4302537.4366636/1‑94 (MQ=60)

                          GTTCAGCAGCGTTGTTGCCAGCGTATTTTCAAGGATTTGGATCCGCTTGGTGACAAATGCAGGCGCCTGCCCAAGCGTCGCCGCTGCGGCGCTA                                                                   <  SRR4302537.126892/94‑1 (MQ=60)

                          GTTCAGCAGCGTTGTTGCCAGCGTATTTTCAAGGATTTGGATCCGCTTGGTGACAAATGCAGGCGCCTGCCCAAGCGTCGCCGCTGCGGCGCTA                                                                   >  SRR4302537.2797099/1‑94 (MQ=60)

                          GTTCAGCAGCGTTGTTGCCAGCGTATTTTCAAGGATTTGGATCCGCTTGGTGACAAATGCAGGCGCCTGCCCAAGCGTCGCCGCTGCGGCGCTA                                                                   >  SRR4302537.1844011/1‑94 (MQ=60)

                           TTCAGCAGCGTTGTTGCCAGCGTATTTTCAAGGATTTGGATCCGCTTGGTGACAAATGCAGGCGCCTGCCCAAGCGTCGCCGCTGCGGCGCTAA                                                                  >  SRR4302537.5316848/1‑94 (MQ=60)

                           TTCAGCAGCGTTGTTGCCAGCGTATTTTCAAGGATTTGGATCCGCTTGGTGACAAATGCAGGCGCCTGCCCAAGCGTCGCCGCTGCGGCGCTAA                                                                  >  SRR4302537.1513403/1‑94 (MQ=60)

                           TTCAGCAGCGTTGTTGCCAGCGTATTTTCAAGGATTTGGATCCGCTTGGTGACAAATGCAGGCGCCTGCCCAAGCGTCGCCGCTGCGGCGCTAA                                                                  <  SRR4302537.6229467/94‑1 (MQ=60)

                            TCAGCAGCGTTGTTGCCAGCGTATTTTCAAGGATTTGGATCCGCTTGGTGACAAATGCAGGCGTCTGCCCAAGCGTCGCCGCTGCGGCGCTAAA                                                                 <  SRR4302537.1738976/94‑1 (MQ=60)

                             CAGCAGCGTTGTTGCCAGCGTATTTTCAAGGATTTGGATCCGCTTGGTGACAAATGCAGGCGTCTGCCCAAGCGTCGCCGCTGCGGCGCTAAAA                                                                >  SRR4302537.3789300/1‑94 (MQ=60)

                             CAGCAGCGTTGTTGCCAGCGTATTTTCAAGGATTTGGATCCGCTTGGTGACAAATGCAGGCGCCTGCCCAAGCGTCGCCGCTGCGGCGCTAAAA                                                                >  SRR4302537.2273445/1‑94 (MQ=60)

                             CAGCAGCGTTGTTGCCAGCGTATTTTCAAGGATTTGGATCCGCTTGGTGACAAATGCAGGCGCCTGCCCAAGCGTCGCCGCTGCGGCGCTAAAA                                                                >  SRR4302537.2082723/1‑94 (MQ=60)

                              AGCAGCGTTTTTGCCAGCGTATTTTCAAGGATTTGGATCCGCTTGGTGACAAATGCAGGCGCCTGCCCAAGCGTCGCCGCTGCGGCGCTAAAAC                                                               <  SRR4302537.860281/94‑1 (MQ=60)

                              AGCAGCGTTGTTGCCAGCGTATTTTCAAGGATTTGGATCCGCTTGGTGACAAATGCAGGCGTCTGCCCAAGCGTCGCCGCTGCGGCGCTAAAAC                                                               <  SRR4302537.4316003/94‑1 (MQ=60)

                              AGCAGCGTTGTTGCCAGCGTATTTTCAAGGATTTGGATCCGCTTGGTGACAAATGCAGGCGCCTGCCCAAGCGTCGCCGCTGCGGCGCTAAAAC                                                               <  SRR4302537.3149653/94‑1 (MQ=60)

                              AGCAGCGTTGTTGCCAGCGTATTTTCAAGGATTTGGATCCGCTTGGTGACAAATGCAGGCGCCTGCCCAAGCGTCGCCGCTGCGGCGCTAAAAC                                                               >  SRR4302537.656397/1‑94 (MQ=60)

                                CAGCGTTGTTGCCAGCGTATTTTCAAGGATTTGGATCCGCTTGGTGACAAATGCAGGCGCCTGCCCAAGCGTCGCCGCTGCGGCGCTAAAACTC                                                             >  SRR4302537.3361472/1‑94 (MQ=60)

                                 AGCGTTGTTGCCAGCGTATTTTCAAGGATTTGGATCCGCTTGGTGACAAATGCAGGCGCCTGCCCAAGCGTCGCCGCTGCGGCGCTAAAACTAC                                                            >  SRR4302537.2994548/1‑94 (MQ=60)

                                  GCGTTGTTGCCAGCGTATTTTCAAGGATTTGGATCCGCTTGGTGACAAATGCAGGCGCCTGCCCAAGCGTCGCCGCTGCGGCGCTAAAACTCCC                                                           <  SRR4302537.2000169/94‑1 (MQ=60)

                                   CGTTGTTGCCAGCGTATTTTCAAGGATTTGGATCCGCTTGGTGACAAATGCAGGCGCCTGCCCAAGCGTCGCCGCTGCGGCGCTAAAACTCCCG                                                          <  SRR4302537.2280543/94‑1 (MQ=60)

                                    GTTGTTGCCAGCGTATTTTCAAGGATTTGGATCCGCTTGGTGACAAATGCAGGCGCCTGCCCAAGCGTCGCNGCTGCGGCGCTAAAAgtacngt                                                         >  SRR4302537.4560878/1‑87 (MQ=60)

                                    GTTGTTGCCAGCGTATTTTCAAGGATTTGGATCCGCTTGGTGACAAATGCAGGCGCCTGCCCAAGCGTCGCCGCTGCGGCGCTAAAACTCCCGC                                                         >  SRR4302537.1492542/1‑94 (MQ=60)

                                    GTTGTTGCCAGCGTATTTTCAAGGATTTGGATCCGCTTGGTGACAAATGCAGGCGCCTGCCCAAGCGTCGCCGCTGCGGCGCTAAAACTCCCGC                                                         >  SRR4302537.6156459/1‑94 (MQ=60)

                                    GTTGTTGCCAGCGTATTTTCAAGGATTTGGATCCGCTTGGTGACAAATGCAGGCGCCTGCCCAAGCGTCGCCGCTGCGGCGCTAAAACTCCCGC                                                         >  SRR4302537.850742/1‑94 (MQ=60)

                                    GTTGTTGCCAGCGTATTTTCAAGGATTTGGATCCGCTTGGTGACAAATGCAGGCGCCTGCCCAAGCGTCGCCGCTGCGGCGCTAAAACTCCCGC                                                         >  SRR4302537.198970/1‑94 (MQ=60)

                                     TTGTTGCCAGCGTATTTTCAAGGATTTGGATCCGCTTGGTGACAAATGCAGGCGCCTGCCCAAGCGTCGCCGCTGCGGCGCTAAAACTCCCGCT                                                        >  SRR4302537.2540517/1‑94 (MQ=60)

                                       GTTGCCAGCGTATTTTCAAGGATTTGGATCCGCTTGGTGACAAATGCAGGCGCCTGCCCAAGCGTCGCCGCTGCGGCGCTAAAACTCCCGCTGT                                                      <  SRR4302537.5302892/94‑1 (MQ=60)

                                        TTGCCAGCGTATTTTCAAGGATTTGGATCCGCTTGGTGACAAATGCAGGCGCCTGCCCAAGCGTCGCCGCTGCGGCGCTAAAACTCCCGCTGTG                                                     <  SRR4302537.5684683/94‑1 (MQ=60)

                                        TTGCCAGCGTATTTTCAAGGATTTGGATCCGCTTGGTGACAAATGCAGGCGCCTGCCCAAGCGTCGCCGCTGCGGCGCTAAAACTCCCGCTGTG                                                     <  SRR4302537.939698/94‑1 (MQ=60)

                                           CCAGCGTATTTTCAAGGATTTGGATCCGCTTGGTGACAAATGCAGGCGCCTGCCCAAGCGTCGCCGCTGCGGCGCTAAAACTCCCGCTGTGAAC                                                  <  SRR4302537.5321772/94‑1 (MQ=60)

                                           CCAGCGTATTTTCAAGGATTTGGATCCGCTTGGTGACAAATGCAGGCGCCTGCCCAAGCGTCGCCGCTGCGGCGCTAAAACTCCCGCTGTGAAC                                                  <  SRR4302537.1847235/94‑1 (MQ=60)

                                           CCAGCGTATTTTCAAGGATTTGGATCCGCTTGGTGACAAATGCAGGCGCCTGCCCAAGCGTCGCAGCTGCGGCGCTAAAACTCCCGCTGTGAAC                                                  >  SRR4302537.2737277/1‑94 (MQ=60)

                                            CAGCGTATTTTCAAGGATTTGGATCCGCTTGGTGACAAATGCAGGCGTCTGCCCAAGCGTCGCCGCTGCGGCGCTAAAACTCCCGCTGTGAACG                                                 >  SRR4302537.2967863/1‑94 (MQ=60)

                                            CAGCGTATTTTCAAGGATTTGGATCCGCTTGGTGACAAATGCAGGCGCCTGCCCAAGCGTCGCCGCTGCGGCGCTAAAACTCCCGCTGTGAACG                                                 >  SRR4302537.1987432/1‑94 (MQ=60)

                                            CAGCGTATTTTCAAGGATTTGGATCCGCTTGGTGACAAATGCAGGCGCCTGCCCAAGCGTCGCCGCTGCGGCGCTAAAACTCCCGCTGTGAACG                                                 <  SRR4302537.6128635/94‑1 (MQ=60)

                                            CAGCGTATTTTCAAGGATTTGGATCCGCTTGGTGACAAATGCAGGCGCCTGCCCAAGCGTCGCCGCTGCGGCGCTAAAACTCCCGCTGTGAACG                                                 >  SRR4302537.3393465/1‑94 (MQ=60)

                                            CAGCGTATTTTCAAGGATTTGGATCCGCTTGGTGACAAATGCAGGCGCCTGCCCAAGCGTCGCCGCTGCGGCGCTAAAACTCCCGCTGTGAACG                                                 >  SRR4302537.5415743/1‑94 (MQ=60)

                                            CAGCGTATTTTCAAGGATTTGGATCCGCTTGGTGACAAATGCAGGCGCCTGCCCAAGCGTCGCCGCTGCGGCGCTAAAACTCCCGCTGTGAACG                                                 <  SRR4302537.5682382/94‑1 (MQ=60)

                                            CAGCGTATGTTCAAGGATTTGGATCCGCTTGGTGACAAATGCAGGCGCCTGCCCAAGCGTCGCCGCTGCGGCGCTAAAACTCCCGCTGTGAACG                                                 <  SRR4302537.5891387/94‑1 (MQ=60)

                                             AGCGTATTTTCAAGGATTTGGATCCGCTTGGTGACAAATGCAGGCGCCTGCCCAAGCGTCGCCGCTGCGGCGCTAAAACTCCCGCTGTGAACGA                                                >  SRR4302537.5089529/1‑94 (MQ=60)

                                             AGCGTATTTTCAAGGATTTGGATCCGCTTGGTGACAAATGCAGGCGCCTGCCCAAGCGTCGCCGCTGCGGCGCTAAAACTCCCGCTGTGAACGA                                                >  SRR4302537.5540190/1‑94 (MQ=60)

                                             AGCGTATTTTCAAGGATTTGGATCCGCTTGGTGACAAATGCAGGCGCCTGCCCAAGCGTCGCCGCTGCGGCGCTAAAACTCCCGCTGTGAACGA                                                >  SRR4302537.4388766/1‑94 (MQ=60)

                                               CGTATTTTCAAGGATTTGGATCCGCTTGGTGACAAATGCAGGCGCCTGCCCAAGCGTCGCCGCTGCGGCGCTAAAACTCCCGCTGTGAACGATT                                              >  SRR4302537.6270697/1‑94 (MQ=60)

                                                GTATTTTCAAGGATTTGGATCCGCTTGGTGACAAATGCAGGCGCCTGCCCAAGCGTCGCCTCTGCGGCGCTAAAACTCCCGCTGTGAACGATTT                                             >  SRR4302537.652579/1‑94 (MQ=60)

                                                GTATTTTCAAGGATTTGGATCCGCTTGGTGACAAATGCAGGCGCCTGCCCAAGCGTCGCCGCTGCGGCGCTAAAACTCCCGCTGTGAACGATTT                                             <  SRR4302537.2147489/94‑1 (MQ=60)

                                                 TATTTTCAAGGATTTGGATCCGCTTGGTGACAAATGCAGGCGCCTGCCCAAGCGTCGTCGCTGCATCGCTAAAACTCCCGCTGTGAACGATTTG                                            >  SRR4302537.3394046/1‑94 (MQ=60)

                                                 TATTTTCAAGGATTTGGATCCGCTTGGTGACAAATGCAGGCGCCTGCCCAAGCGTCGCCGCTGCGGCGCTAAAACTCCCGCTGTGAACGATTTC                                            >  SRR4302537.4320969/1‑94 (MQ=60)

                                                  ATTTTCAAGGATTTGGATCCGCTTGGTGACAAATGCAGGCGCCTGCCCAAGCGTCGCCGCTGCGGCGCTAAAACTCCCGCTGTGAACGATTTCT                                           <  SRR4302537.4845292/94‑1 (MQ=60)

                                                  ATTTTCAAGGATTTGGATCCGCTTGGTGACAAATGCAGGCGCCTGCCCAAGCGTCGCCGCTGCGGCGCTAAAACTCCCGCTGTGAACGATTTCA                                           >  SRR4302537.5534268/1‑94 (MQ=60)

                                                   TTTTCAAGGATTTGGATCCGCTTGGTGACAAATGCAGGCGCCTGCCCAAGCGTCGCCGCTGCGGCGCTAAAACTCCCGCTGTGAACGATTTCTA                                          >  SRR4302537.2149899/1‑94 (MQ=60)

                                                   TTTTCAAGGATTTGGATCCGCTTGGTGACAAATGCAGGCGCCTGCCCAAGCGTCGCCGCTGCGGCGCTAAAACTCCCGCTGTGAACGATTTCTA                                          >  SRR4302537.3907481/1‑94 (MQ=60)

                                                   TTTTCAAGGATTTGGATCCGCTTGGTGACAAATGCAGGCGCCTGCCCAAGCGTCGCCGCTGCGGCGCTAAAACTCCCGCTGTGAACGATTTCTA                                          >  SRR4302537.4667644/1‑94 (MQ=60)

                                                     TTCAAGGATTTGGATCCGCTTGGTGACAAATGCAGGCGCCTGCCCAAGCGTCGCCGCTGCGGCGCTAAAACTCCCGCTGTGAACGATTTCTACC                                        >  SRR4302537.5501493/1‑94 (MQ=60)

                                                      TCAAGGATTTGGATCCGCTTGGTGACAAATGCAGGCGCCTGCCCAAGCGTCGCCGCTGCGGCGCTAAAACTCCCGCTGTGAACGATTTCTACCA                                       <  SRR4302537.4552855/94‑1 (MQ=60)

                                                       CAAGGATTTGGATCCGCTTGGTGACAAATGCAGGCGCCTGCCCAAGCGTCGCCGCTGCGGCGCTAAAACTCCCGCTGTGAACGATTTCTACCAG                                      >  SRR4302537.3786714/1‑94 (MQ=60)

                                                       CAAGGATTTGGATCCGCTTGGTGACAAATGCAGGCGCCTGCCCAAGCGTCGCCGCTGCGGCGCTAAAACTCCCGCTGTGAACGATTTCTACCAG                                      >  SRR4302537.849230/1‑94 (MQ=60)

                                                       CAAGGATTTGGATCCGCTTGGTGACAAATGCAGGCGCCTGCCCAAGCGTCGCCGCTGCGGCGCTAAAACTCCCGCTGTGAACGATTTCTACCAG                                      >  SRR4302537.4418352/1‑94 (MQ=60)

                                                        AAGGATTTGGATCCGCTTGGTGACAAATGCAGGCGCCTGCCCAGGCGTCGCCGCTGCGGCGCTAAAACTCCCGCTGTGAACGATTTCTACCAGT                                     >  SRR4302537.3482439/1‑94 (MQ=60)

                                                           GATTTGGATCCGCTTGGTGACAAATGCAGGCGCCTGCCCAAGCGTCGCCGCTGCGGCGCTAAAACTCCCGCTGTGAACGATTTCTACCAGTACC                                  >  SRR4302537.2077247/1‑94 (MQ=60)

                                                           GATTTGGATCCGCTTGGTGACAAATGCAGGCGCCTGCCCAAGCGTCGCCGCTGCGGCGCTAAAACTCCCGCTGTGAACGATTTCTACCAGTACC                                  >  SRR4302537.6161782/1‑94 (MQ=60)

                                                             TTTGGATCCGCTTGGTGACAAATGCAGGCGCCTGCCCAAGCGTCGCCGCTGCGGCGCTAAAACTCCCGCTGTGAACGATTTCTACCAGTACCTG                                <  SRR4302537.6267496/94‑1 (MQ=60)

                                                               TGGATCCGCTTGGTGACAAATGCAGGCGCCTGCCCAAGCGTCGCCGCTGCGGCGCTAAAACTCCCGCTGTGAACGATTTCTACCAGTACCTGCA                              <  SRR4302537.4566426/94‑1 (MQ=60)

                                                                 GATCCGCTTGGTGACAAATGCAGGCGCCTGCCCAAGCGTCGCCGCTGCGGCGCTAAAACTCCCGCTGTGAACGATTTCTACCAGTACCTGCAAG                            <  SRR4302537.1197872/94‑1 (MQ=60)

                                                                  ATCCGCTTGGTGACAAATGCAGGCGCCTGCCCAAGCGTCGCCGCTGCGGCGCTAAAACTCCCGCTGTGAACGATTTCTACCAGTACCTGCAAGT                           >  SRR4302537.6161348/1‑94 (MQ=60)

                                                                  ATCCGCTTGGTGACAAATGCAGGCGCCTGCCCAAGCGTCGCCGCTGCGGCGCTAAAACTCCAGCTGTGAACGATTTCTACCAGTACCTGCAAGT                           <  SRR4302537.4529707/94‑1 (MQ=60)

                                                                   TCCGCTTGGTGACAAATGCAGGCGCCTGCCCAAGCGTCGCCGCTGCGGCGCTAAAACTCCCGCTGTGAACGATTTCTACCAGTACCTGCAAGTC                          >  SRR4302537.2546121/1‑94 (MQ=60)

                                                                    CCGCTTGGTGACAAATGCAGGCGCCTGCCCAAGCGTCGCCGCTGCGGCGCTAAAACTCCCGCTGTGAACGATTTCTACCAGTACCTGCAAGTCT                         >  SRR4302537.4548994/1‑94 (MQ=60)

                                                                    CCGCTTGGTGACAAATGCAGGCGCCTGCCCAAGCGTCGCCGCTGCGGCGCTAAAACTCCCGCTGTGAACGATTTCTACCAGTACCTGCAAGTCT                         >  SRR4302537.3556055/1‑94 (MQ=60)

                                                                     CGCTTGGTGACAAATGCAGGCGCCTGCCCAAGCGTCGCCGCTGCGGCGCTAAAACTCCCGCTGTGAACGATTTCTACCAGTACCTGCAAGTCTT                        >  SRR4302537.5365076/1‑94 (MQ=60)

                                                                     CGCTTGGTGACAAATGCAGGCGCCTGCCCAAGCGTCGCCGCTGCGGCGCTAAAACTCCCGCTGTGAACGATTTCTACCAGTACCTGCAAGTCTT                        >  SRR4302537.3585797/1‑94 (MQ=60)

                                                                     CGCTTGGTGACAAATGCAGGCGCCTGCCCAAGCGTCGCCGCTGCGGCGCTAAAACTCCCGCTGTGAACGATTTCTACCAGTACCTGCAAGTCTT                        <  SRR4302537.3128194/94‑1 (MQ=60)

                                                                     CGCTTGGTGACAAATGCAGGCGCCTGCCCAAGCGTCGCCGCTGCGGCGCTAAAACTCCCGCTGTGAACGATTTCTACCAGTACCTGCAAGTCTT                        >  SRR4302537.416183/1‑94 (MQ=60)

                                                                      GCTTGGTGACAAATGCAGGCGCCTGCCCAAGCGTCGCCGCTGCGGCGCTAAAACTCCCGCTGTGAACGATTTCTACCAGTACCTGCAAGTCTTT                       >  SRR4302537.2597380/1‑94 (MQ=60)

                                                                       CTTGGTGCCAAATGCAGGCGCCTGCCCAAGCGTCGCCGCTGCGGCGCTAAAACTCCCGCTGTGAACGATTTCTACCAGTACCTGCAAGTCTTTG                      <  SRR4302537.4717214/94‑1 (MQ=60)

                                                                       CTTGGTGACAAATGCAGGCGCCTGCCCAAGCGTCGCCGCTGCGGCGCTAAAACTCCCGCTGTGAACGATTTCTACCAGTACCTGCAAGTCTTTG                      <  SRR4302537.5140979/94‑1 (MQ=60)

                                                                       CTTGGTGACAAATGCAGGCGCCTACCCAAGCGTCGCCGCTGCGGCGCTAAAACTCCCGCTGTGAACGATTTCTACCAGTACCTGCAAGTCTTTG                      <  SRR4302537.2117933/94‑1 (MQ=60)

                                                                        TTGGTGACAAATGCAGGCGCCTGCCCAAGCGTCGCCGCTGCGGCGCTAAAACTCCCGCTGTGAACGATTTCTACCAGTACCTGCAAGTCTTTGG                     <  SRR4302537.2716553/94‑1 (MQ=60)

                                                                         TGGTGACAAATGCAGGCGCCTGCCCAAGCGTCGCCGCTGCGGCGCTAAAACTCCCGCTGTGAACGATTTCTACCAGTACCTGCAAGTCTTTGGC                    <  SRR4302537.5517585/94‑1 (MQ=60)

                                                                         TGGTGACAAATGCAGGCGCCTGCCCAAGCGTCGCCGCTGCGGCGCTAAAACTCCCGCTGTGAACGATTTCTACCAGTACCTGCAAGTCTTTGGC                    >  SRR4302537.5056398/1‑94 (MQ=60)

                                                                          GGTGACAAATGCAGGCGCCTGCCCAAGCGTCGCCGCTGCGGCGCTAAAAATCCCGCTGTGAACGATTTCTACCAGTACCTGCAAGTCTTTGGCT                   >  SRR4302537.1783677/1‑94 (MQ=60)

                                                                             GACAAATGCAGGCGCCTGCCCAAGCGTCGCCGCTGCGGCGCTAAAACTCCCGCTGTGAACGATTTCTACCAGTACCTGCAAGTCTTTGGCTAAA                <  SRR4302537.4018647/94‑1 (MQ=60)

                                                                              ACAAATGCAGGCGCCTGCCCAAGCGTCGCCGCTGCGGCGCTAAAACTCCCGCTGTGAACGATTTCTACCAGTACCTGCAAGTCTTTGGCTAAAG               >  SRR4302537.86003/1‑94 (MQ=60)

                                                                              ACAAATGCAGGCGCCTGCCCAAGCGTCGCCGCTGCGGCGCTAAAACTCCCGCTGTGAACGATTTCTACCAGTACCTGCAAGTCTTTGGCTAAAG               >  SRR4302537.3058075/1‑94 (MQ=60)

                                                                              ACAAATGCAGGCGCCTGCCCAAGCGTCGCCGCTGCGGCGCTAAAACTCCCGCTGTGAACGATTTCTACCAGTACCTGCAAGTCTTTGGCTAAAG               <  SRR4302537.3678697/94‑1 (MQ=60)

                                                                               CAAATGCAGGCGCCTGCCCAAGCGTCGCCGCTGCGGCGCTAAAACTCCCGCTGTGAACGATTTCTACCAGTACCTGCAAGTCTTTGGCTAAAGG              <  SRR4302537.6379017/94‑1 (MQ=60)

                                                                                AAATGCAGGCGCCTGCCCAAGCGTCGCCGCTGCGGCGCTAAAACTCCCGCTGTGAACGATTTCTACCAGTACCTGCAAGTCTTTGGCTAAAGGC             <  SRR4302537.3779588/94‑1 (MQ=60)

                                                                                 AATGCAGGCGCCTGCCCAAGCGTCGCCGCTGCGGCGCTAAAACTCCCGCTGTGAACGATTTCTACCAGTACCTGCAAGTCTTTGGCTAAAGGCC            >  SRR4302537.3627843/1‑94 (MQ=60)

                                                                                 AATGCAGGCGCCTGCCCAAGCGTCGCCGCTGCGGCGCTAAAACTCCCGCTGTGAACGATTTCTACCAGTACCTGCAAGTCTTTGGCTAAAGGCC            <  SRR4302537.861108/94‑1 (MQ=60)

                                                                                  ATGCAGGCGCCTGCCCAAGCGTCGCCGCTGCGGCGCTAAAACTCCCGCTGTGAACGATTTCTACCAGTACCTGCAAGTCTTTGGCTAAAGGCCA           >  SRR4302537.3946906/1‑94 (MQ=60)

                                                                                   TGCAGGCGCCTGCCCAAGCGTCGCCGCTGCGGCGCTAAAACTCCCGCTGTGAACGATTTCTACCAGTACCTGCAAGTCTTTGGCTAAAGGCCAG          <  SRR4302537.3587662/94‑1 (MQ=60)

                                                                                   TGCAGGCGCCTGCCCAAGCGTCGCCGCTGCGGCGCTAAAACTCCCGCTGTGAACGATTTCTACCAGTACCTGCAAGTCTTTGGCTAAAGGCCAG          <  SRR4302537.2018239/94‑1 (MQ=60)

                                                                                    GCAGGCGCCTGCCCAAGCGTCGCCGCTGCGGCGCTAAAACTCCCGCTGTGAACGATTTCTACCAGTACCTGCAAGTCTTTGGCTAAAGGCCAGC         >  SRR4302537.8398/1‑94 (MQ=60)

                                                                                    GCAGGCGCCTGCCCAAGCGTCGCCGCTGCGGCGCTAAAACTCCCGCTGTGAACGATTTCTACCAGTACCTGCAAGTCTTTGGCTAAAGGCCAGC         <  SRR4302537.4797505/94‑1 (MQ=60)

                                                                                      AGGCGCCTGCCCAAGCGTCGCCGCTGCGGCGCTAAAACTCCCGCTGTGAACGATTTCTACCAGTACCTGCAAGTCTTTGGCTAAAGGCCAGCTA       <  SRR4302537.2886541/94‑1 (MQ=60)

                                                                                       GGCGACTGCCCAAGCGTCGCCGCTGCGGCGCTAAAACTCCCGCTGTGAACGATTTCTACCAGTACCTGCAAGTCTTTGGCTAAAGGCCAGCTAT      <  SRR4302537.3092993/94‑1 (MQ=60)

                                                                                        GCGCCTGCCCAAGCGTCGCCGCTGCGGCGCTAAAACTCCCGCTGTGAACGATTTCTACCAGTACCTGCAAGTCTTTGGCTAAAGGCCAGCTATT     >  SRR4302537.3801159/1‑94 (MQ=60)

                                                                                        GCGCCTGCCCAAGCGTCGCCGCTGCGGCGCTAAAACTCCCGCTGTGAACGATTTCTACCAGTACCTGCAAGTCTTTGGCTAAAGGCCAGCTATT     <  SRR4302537.2933126/94‑1 (MQ=60)

                                                                                         CGCCTGCCCAAGCGTCGCCGCTGCGGCGCTAAAACTCCCGCTGTGAACGATTTCTACCAGTACCTGCAAGTCTTTGGCTAAAGGCCAGCTATTC    >  SRR4302537.4602307/1‑94 (MQ=60)

                                                                                           CCTGCCCAAGCGTCGCCGCTGCGGCGCTAAAACTCCCGCTGTGAACGATTTCTACCAGTACCTGCAAGTCTTTGGCTAAAGGCCAGCTATTCAG  >  SRR4302537.5946274/1‑94 (MQ=60)

GTCAGCGCCACGCCGCGAGCGGAGCGGTTCAGCAGCGTTGTTGCCAGCGTATTTTCAAGGATTTGGATCCGCTTGGTGACAAATGCAGGCGTCTGCCCAAGCGTCGCCGCTGCGGCGCTAAAACTCCCGCTGTGAACGATTTCTACCAGTACCTGCAAGTCTTTGGCTAAAGGCCAGCTATTCAG  >  NC_000913/3203710‑3203894

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 0 ≤` `ATCG/ATCG` `< 2 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`