Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I2 R1 882 254.4 12957588 98.0% 12698436 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,236,758 Δ1 bp coding (537/966 nt) ygjT → putative transport protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,236,7570T.100.0% 1098.6 / NA 234coding (536/966 nt)ygjTputative transport protein
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base . (110/124);  total (110/124)

Only 100 of 261 total aligned reads displayed.

GCCTTCCTGCTGTTTACCGGCGTGAAGATGGCGCTCGCCCATGAAGATGAATCAGGCATTGGCGACAAG‑C‑GCTGGTGCGCTGGCTACGCGGTTCATTTGCGCATGACCGACACCATCGACAACGAGCATTTCTTTGTGCGTAAGAATGGATTGCTGTACGCCACACCGCTGATGCTGGTA  >  NC_000913/3236666‑3236845
                                                                                             |                                                                                        
gCCTTCCTGCTGTTTACCGGCGTGAAGATGGCGCTCGCCCATGAAGATGAATCAGGCATTGGCGACAAGCC‑GCTGGTGCGCTGGCTACGCGGt                                                                                          >  1:7894/1‑93 (MQ=255)
gCCTTCCTGCTGTTTACCGGCGTGAAGATGGCGCTCGCCCATGAAGATGAATCAGGCATTGGCGACAAGCC‑GCTGGTGCGCTGGCTACGCGGt                                                                                          >  2:4270100/1‑93 (MQ=255)
gCCTTCCTGCTGTTTACCGGCGTGAAGATGGCGCTCGCCCATGAAGATGAATCAGGCATTGGCGACAAGCC‑GCTGGTGCGCTGGCTACGCGGt                                                                                          >  1:6014488/1‑93 (MQ=255)
  cTTCCTGCTGTTTACCGGCGTGAAGATGGCGCTCGCCCATGAAGATGAATCAGGCATTGGCGACAAGCC‑GCTGGTGCGCTGGCTACGCGGtca                                                                                        >  2:4081618/1‑91 (MQ=255)
  cTTCCTGCTGTTTACCGGCGTGAAGATGGCGCTCGCCCATGAAGATGAATCAGGCATTGGCGACAAGCC‑GCTGGTGCGCTGGCTACGCGGtca                                                                                        >  1:4222428/1‑91 (MQ=255)
  cTTCCTGCTGTTTACCGGCGTGAAGATGGCGCTCGCCCATGAAGATGAATCAGGCATTGGCGACAAGCC‑GCTGGTGCGCTGGCTACGCGGtca                                                                                        <  2:2305530/93‑3 (MQ=255)
   ttCCTGCTGTTTACCGGCGTGAAGATGGCGCTCGCCCATGAAGATGAATCAGGCATTGGCGACAAGCC‑GCTGGTGCGCTGGCTACGCGGtcat                                                                                       <  1:5905215/93‑4 (MQ=255)
   ttCCTGCTGTTTACCGGCGTGAAGATGGCGCTCGCCCATGAAGATGAATCAGGCATTGGCGACAAGCC‑GCTGGTGCGCTGGCTACGCGGtcat                                                                                       <  1:1867554/93‑4 (MQ=255)
   ttCCTGCTGTTTACCGGCGTGAAGATGGCGCTCGCCCATGAAGATGAATCAGGCATTGGCGACAAGCC‑GCTGGTGCGCTGGCTACGCGGtcat                                                                                       >  1:978648/1‑90 (MQ=255)
    tCCTGCTTTTTACCGGCGTGAAGAGGGCCTTCGCCCATGAAGATGACTCGGGCATTGGCGACAAGCC‑GCTGGTGCGCTGGCTACGCGGtcatt                                                                                      <  2:179204/93‑5 (MQ=255)
     ccTGCTGTTTACCGGCGTGAAGATGGCGCTCGCCCATGAAGATGAATCAGGCATTGGCGACAAGCC‑GCTGGTGCGCTGGCTACGCGG‑TCAttt                                                                                    <  2:6118522/93‑1 (MQ=255)
      ctgctgTTTACCGGCGTGAAGATGGCGCTCGCCCATGAAGATGAATCAGGCATTGGCGACAAGCC‑GCTGGTGCGCTGGCTACGCGG‑TCATTTg                                                                                   <  1:1958460/93‑1 (MQ=255)
      ctgctgTTTACCGGCGTGAAGATGGCGCTCGCCCATGAAGATGAATCAGGCATTGGCGACAAGCC‑GCTGGTGCGCTGGCTACGCGG‑TCATTTg                                                                                   >  2:3982466/1‑93 (MQ=255)
       tgttgTTTCCCGGCGTCAAGATGGCGCTCGCCCATGAAGATGAATCAGCCATTGGCGACAAGCC‑GCTGGTGCGCTGGCTACGCGG‑TCATTTgc                                                                                  <  1:363298/90‑1 (MQ=255)
       tgctgTTTACCGGCGTGAAGATGGCGCTCGCCCATGAAGATGAATCAGGCATTGGCGACAAGCC‑GCTGGTGCGCTGGCTACGCGG‑TCATTTgc                                                                                  >  1:4490521/1‑93 (MQ=255)
         ctgTTTACCGGCGTGAAGATGGCGCTCGCCCATGAAGATGAATCAGGCATTGGCGACAAGCC‑GCTGGTGCGCTGGCTACGCGG‑TCATTTgcgc                                                                                <  2:685803/93‑1 (MQ=255)
          tgTTTACCGGCGTGAAGATGGCGCTCGCCCATGAAGATGAATCAGTCATTGGCGACAAGCC‑GCTGGTGCGCTGGCTACGCGG‑TCATTTGCGCa                                                                               <  1:2216257/93‑1 (MQ=255)
          tgTTTACCGGCGTGAAGATGGCGCTCGCCCATGAAGATGAATCAGGCATTGGCGACAAGCC‑GCTGGTGCGCTGGCTACGCGG‑TCATTTGCGCa                                                                               >  1:6471551/1‑93 (MQ=255)
          tgTTTACCGGCGTGAAGATGGCGCTCGCCCATGAAGATGAATCAGGCATTGGCGACAAGCC‑GCTGGTGCGCTGGCTACGCGG‑TCATTTGCGCa                                                                               <  1:3133598/93‑1 (MQ=255)
          tgTTTACCGGCGTGAAGATGGCGCTCGCCCATGAAGATGAATCAGGCATTGGCGACAAGCC‑GCTGGTGCGCTGGCTACGCGG‑TCATTTGCGCa                                                                               >  2:6219495/1‑93 (MQ=255)
            tttACCGGCGTGAAGATGGCGCTCGCCCATGAAGATGAATCAGGCATTGGCGACAAGCC‑GCTGGTGCGCTGGCTACGCGG‑TCATTTGCGCATg                                                                             >  2:531027/1‑93 (MQ=255)
             ttACCGGCGTGAAGATGGCGCTCGCCCATGAAGATGAATCAGGCATTGGCGACAAGCC‑GCTGGTGCGCTGGCTACGCGG‑TCCTTTGCGCATGa                                                                            >  1:3303948/1‑93 (MQ=255)
               tccGGCGTGAAGATGGCGCTCGCCCATGAAGATGAATCAGGCATTGGCGACAAGCC‑GCTGGTGCGCTGGCTACGCGG‑TCATTTGCGCATGAcc                                                                          <  1:4302986/92‑1 (MQ=255)
               aCCGGCGTGAAGATGGCGCTCGCCCATGAAGATGAATCAGGCATTGGCGACAAGCC‑GCTGGTGCGCTGGCTACGCGG‑TCATTTGCGCATGAcc                                                                          <  2:4145220/93‑1 (MQ=255)
                 cGGCGTGAAGATGGCGCTCGCCCATGAAGATGAATCAGGCATTGGCGACAAGCC‑GCTGGTGCGCTGGCTACGCGG‑TCATTTGCGCATGACCGa                                                                        <  2:1702140/93‑1 (MQ=255)
                  ggCGTGAAGATGGCGCTCGCCCATGAAGATGAATCAGGCATTGGCGACAAGCC‑GCTGGTGCGCTGGCTACGCGG‑TCATTTGCGCATGACCGac                                                                       >  1:1049630/1‑93 (MQ=255)
                   gCGTGAAGATGGCGCTCGCCCATGAAGATGAATCAGGCATTGGCGACAAGCC‑GCTGGTGCGCTGGCTACGCGG‑TCATTTGCGCATGACCGaca                                                                      >  2:354148/1‑93 (MQ=255)
                   gCGTGAAGATGGCGCTCGCCCATGAAGATGAATCAGGCATTGGCGACAAGCC‑GCTGGTGCGCTGGCTACGCGG‑TCATTTGCGCATGACCGaca                                                                      >  1:1379126/1‑93 (MQ=255)
                    cGTGAAGATGGCGCTCGCCCATGAAGATGGATCAGGCATTGGCGACAAGCC‑GCTGGTGCGCTGGCTCCGCGG‑TCATTTGCGCATGACCGacac                                                                     >  2:4347620/1‑93 (MQ=255)
                     gTGAAGATGGCGCTCGCCCATGAAGATGAATCAGGCATTGGCGACAAGCC‑GCTGGTGCGCTGGCTACGCGG‑TCATTTGCGCATGACCGACAcc                                                                    <  1:5807152/93‑1 (MQ=255)
                     gTGAAGATGGCGCTCGCCCATGAAGATGAATCAGGCATTGGCGACAAGCC‑GCTGGTGCGCTGGCTACGCGG‑TCATTTGCGCATGACCGACAcc                                                                    <  1:4203552/93‑1 (MQ=255)
                      tGAAGATGGCGCTCGCCCATGAAGATGAATCAGGCATTGGCGACAAGCC‑GCTGGTGCGCTGGCTACGCGG‑TCATTTGCGCATGACCGACACCa                                                                   <  2:1343435/93‑1 (MQ=255)
                       gAAGATGGCGCTCGCCCATGAAGATGAATCAGGCATTGGCGACAAGCC‑GCTGGTGCGCTGGCTACGCGG‑TCATTTGCGCATGACCGACACCAt                                                                  <  2:1168932/93‑1 (MQ=255)
                       gAAGATGGCGCTCGCCCATGAAGATGAATCAGGCATTGGCGACAAGCC‑GCTGGTGCGCTGGCTACGCGG‑TCATTTGCGCATGACCGACACCAt                                                                  >  1:6135407/1‑93 (MQ=255)
                        aaGATGGCGCTCGCCCATGAAGATGAATCAGGCATTGGCGACAAGCC‑GCTGGTGCGCTGGCTACGCGG‑TCATTTGCGCATGACCGACACCATc                                                                 >  2:2995605/1‑93 (MQ=255)
                        aaGATGGCGCTCGCCCATGAAGATGAATCAGGCATTGGCGACAAGCC‑GCTGGTGCGCTGGCTACGCGG‑TCATTTGCGCATGACCGACACCATc                                                                 <  1:2485797/93‑1 (MQ=255)
                         aGATGGCGCTCGCCCATGAAGATGAATCAGGCATTGGCGACAAGCC‑GCTGGTGCGCTGGCTACGCGG‑TCATTTGCGCATGACCGACACCATCg                                                                >  1:3901160/1‑93 (MQ=255)
                         aGATGGCGCTCGCCCATGAAGATGAATCAGGCATTGGCGACAAGCC‑GCTGGTGCGCTGGCTACGCGG‑TCATTTGCGCATGACCGACACCATCg                                                                >  1:82105/1‑93 (MQ=255)
                          gATGGCGCTCGCCCATGAAGATGAATCAGGCATTGGCGACAAG‑CAGCTGGTGCGCTGGCTACGCGG‑CCCTTTGAGCATGACCGACACCATCgc                                                               >  1:1810290/1‑92 (MQ=255)
                           aTGGCGCTCGCCCATGAAGATGAATCAGGCATTGGCGACAAGCC‑GCTGGTGCGCTGGCTACGCGG‑TCATTTGCGCATGACCGACACCATCGAc                                                              >  2:2102192/1‑93 (MQ=255)
                            tGGCGCTCGCCCATGAAGATGAATCAGGCATTGGCGACAAGCC‑GCTGGTGCGCTGGCTACGCGG‑TCATTTGCGCATGACCGACACCATCGACa                                                             >  1:6246310/1‑93 (MQ=255)
                            tGGCGCTCGCCCATGAAGATGAATCAGGCATTGGCGACAAGCC‑GCTGGTGCGCTGGCTACGCGG‑TCATTTGCGCATGACCGACACCATCGACa                                                             >  1:2861550/1‑93 (MQ=255)
                             ggCGCTCGCCCATGAAGATGAATCAGGCATTGGCGACAAGCC‑GCTGGTGCGCTGGCTACGCGG‑TCATTTGCGCATGACCGACACCATCGACaa                                                            <  2:3498807/93‑1 (MQ=255)
                              gcgcgcgcCCATGAAGATGAATCAGGCATTGGCGACAAGCC‑GCTGATGCGCTGGCTACGCGG‑TCATTTGCGCATGACCGACACCATCGACAAc                                                           <  2:2404665/93‑1 (MQ=255)
                              gcgcTCCCCCATGAAGATGAATCAGGCATTGGCGACAAGCC‑GCTGGTGCGCTGGCTACGCGG‑TCATTTGCGCATGACCGACACCATCGACAAc                                                           <  2:2331626/93‑1 (MQ=255)
                               ggcTCGCCCATGAAGATGAATCAGGCATTGGCGACAAGCC‑GCTGGTGCGCTGGCTACGCGG‑TCATTTGCGCATGACCGACACCATCGACAACg                                                          <  1:4887853/92‑1 (MQ=255)
                               cgcTCGCCCATGAAGATGAATCAGGCATTGGCGACAAGCC‑GCTGGTGCGCTGGCTACGCGG‑TCATTTGCGCATGACCGACACCATCGACAACg                                                          <  1:2982463/93‑1 (MQ=255)
                               cgcTCGCCCATGAAGATGAATCAGGCATTGGCGACAAGCC‑GCTGGTGCGCTGGCTACGCGG‑TCATTTGCGCATGACCGACACCATCGACAACg                                                          >  1:5875141/1‑93 (MQ=255)
                                gcTCGCCCATGAAGATGAATCAGGCATTGGCGACAAGCC‑GCTGGTGCGCTGGCTACGCGG‑TCATTTGCGCATGACCGACACCATCGACAACGa                                                         >  1:2583193/1‑93 (MQ=255)
                                 cTCGCCCATGAAGATGAATCAGGCATTGGCGACAAGCC‑GCTGGTGCGCTGGCTACGCGG‑TCATTTGCGCATGACCGACACCATCGACAACGAg                                                        <  1:1044720/93‑1 (MQ=255)
                                  tCGCCCATGAAGATGAATCAGGCATTGGCGACAAGCC‑GCTGGTGCGCTGGCTACGCGG‑TCATTTGCGCATGACCGACACCATCGACAACGAGc                                                       <  1:5512906/93‑1 (MQ=255)
                                   cGCCCATGAACATGAATCAGGCATTGGCGACAAGCC‑GCTGGTGCGCTGGCTACGCGG‑TCATTTGCGCATGACCGACACCATCGACAACGAGCa                                                      <  2:380693/93‑1 (MQ=255)
                                      ccATGAAGATGAATCAGGCATTGGCGACAAGCC‑GCTGGTGCGCTGGCTACGCGG‑TCATTTGCGCATGACCGACACCATCGACAACGAGCAttt                                                   >  1:2912817/1‑93 (MQ=255)
                                        aTGAAGATGAATCAGGCATTGGCGACAAGCC‑GCTGGTGCGCTGGCTACGCGG‑TCATTTGCGCATGACCGACACCATCGACAACGAGCATTTCt                                                 >  1:4343560/1‑93 (MQ=255)
                                        aTGAAGATGAATCAGGCATTGGCGACAAGCC‑GCTGGTGCGCTGGCTACGCGG‑TCATTTGCGCATGACCGACACCATCGACAACGAGCATTTCt                                                 <  2:6078047/93‑1 (MQ=255)
                                         tGAAGATGAATCAGGCATTGGCGACAAGCC‑GCTGGTGCGCTGGCTACGCGG‑TCATTTGCGCATGACCGACACCATCGACAACGAGCATTTCtt                                                <  1:332115/93‑1 (MQ=255)
                                          gAAGATGAATCAGGCATTGGGGACAAGCC‑GCTGGTGCGCTGGCTACGCGG‑TCATTTGCGCATGACCGACACCATCGACAACGAGCATTTCttt                                               <  1:1094233/93‑1 (MQ=255)
                                           aaGATGAATCAGGCATTGGCGACAAGCC‑GCTGGTGCGCTGGCTACGCGG‑TCATTTGCGCATGACCGACACCATCGACAACGAGCATTTCTTtg                                              >  2:3294120/1‑93 (MQ=255)
                                             taTGAATCAGGCATGGCCAACAAGCC‑GCTGGTGCGCTGGCTACGCGG‑TCATTTGCGCATGACCGACACCATCGACAACGAGCATTTCTTtgtg                                            <  1:4829047/92‑1 (MQ=255)
                                             gtgcaaTCGGGCTTTGGAGACAAGCC‑GCTGGTGCGCTGTCTACGCGG‑TCATTTGCGCATGACCGACACCATCGACAACGAGCATTTCTTtgtg                                            <  2:1216388/89‑1 (MQ=255)
                                             gATGAATCAGGCATTGGCGACAAGCC‑GCTGGTGCGCTGGCTACGCGG‑TCATTTGCGCATGACCGACACCATCGACAACGAGCATTTCTTtgtg                                            >  1:155924/1‑93 (MQ=255)
                                              aTGAATCAGGCATTGGCGACAAGCC‑GCTGGTGCGCTGGCTACGCGG‑TCATTTGCGCATGACCGACACCATCGACAACGAGCATTTCTTTGTGc                                           >  1:6336571/1‑93 (MQ=255)
                                                gAATCAGGCATTGGCGACAAGCC‑GCTGGTGCGCTGGCTACGCGG‑TCATTTGCGCATGACCGACACCATCGACAACGAGCATTTCTTTGTGCGt                                         <  2:4511041/93‑1 (MQ=255)
                                                 aaTCAGGCATTGGCGACAAGCC‑GCTGGTGCGCTGGCTACGCGG‑TCATTTGCGCATGACCGACACCATCGACAACGAGCATTTCTTTGTGCGTa                                        >  2:5980390/1‑93 (MQ=255)
                                                  aTCAGGCATTGGCGACAAGCC‑GCTGGTGCGCTGGCTACGCGG‑TCATTTGCGCATGACCGACACCATCGACAACGAGCATTTCTTTGTGCGTaa                                       <  2:5562512/93‑1 (MQ=255)
                                                     aGGCATTGGCGACAAGCC‑GCTGGTGCGCTGGCTACGCGG‑TCATTTGCGCATGACCGACACCATCGACAACGAGCATTTCTTTGTGCGTAAGaa                                    >  2:5685912/1‑93 (MQ=255)
                                                     aGGCATTGGCGACAAGCC‑GCTGGTGCGCTGGCTACGCGG‑TCATTTGCGCATGACCGACACCATCGACAACGAGCATTTCTTTGTGCGTAAGaa                                    >  2:5237500/1‑93 (MQ=255)
                                                         atgggCGACAAGCC‑GCTGGTGCGCTGGCTACGCGG‑TCATTTGCGCATGACCGACACCATCGACAACGAGCATTTCTTTGTGCGTAAGAATGGa                                <  1:2272840/90‑1 (MQ=255)
                                                          ttGGCGACAAGCC‑GCTGGTGCGCTGGCTACGCGG‑TCATTTGCGCATGACCGACACCATCGACAACGAGCATTTCTTTGTGCGTAAGAATGGAt                               <  1:4006767/93‑1 (MQ=255)
                                                          ttGGCGACAAGCC‑GCTGGTGCGCTGGCTACGCGG‑TCATTTGCGCATGACCGACACCATCGACAACGAGCATTTCTTTGTGCGTAAGAATGGAt                               >  2:197201/1‑93 (MQ=255)
                                                           tGGCGACAAGCC‑GCTGGTGCGCTGGCTACGCGG‑TCATTTGCGCATGACCGACACCATCGACAACGAGCATTTCTTTGTGCGTAAGAATGGAtt                              <  1:5108173/93‑1 (MQ=255)
                                                                  aagcc‑gcTGGTGCGCTGGCTACGCGG‑TCATTTGCGCATGACCGACACCATCGACAACGAGCATTTCTTTGTGCGTAAGAATGGATTGCTGTAc                       <  1:5282931/89‑1 (MQ=255)
                                                                    gcc‑gcTGGTGCGCTGGCTACGCGG‑TCATTTGCGCATGACCGACACCATCGACAACGAGCATTTCTTTGTGCGTAAGAATGGATTGCTGTACGc                     <  2:5953814/91‑1 (MQ=255)
                                                                    gcc‑gcTGGTGCGCTGGCTACGCGG‑TCATTTGCGCATGACCGACACCATCGACAACGAGCATTTCTTTGTGCGTAAGAATGGATTGCTGTACGc                     >  2:3151811/3‑93 (MQ=255)
                                                                    gcc‑gcTGGTGCGCTGGCTACGCGG‑TCATTTGCGCATGACCGACACCATCGACAACGAGCATTTCTTTGTGCGTAAGAATGGATTGCTGTACGc                     >  1:4604407/3‑93 (MQ=255)
                                                                      c‑gcTGGTGCGCTGGCTACGCGG‑TCATTTGCGCATGACCGACACCATCGACAACGAGCATTTCTTTGTGCGTAAGAATGGATTGCTGTACGCca                   <  1:2799886/93‑1 (MQ=255)
                                                                      c‑gcTGGTGCGCTGGCTACGCGG‑TCATTTGCGCATGACCGACACCATCGACAACGAGCATTTCTTTGTGCGTAAGAATGGATTGCTGTACGCca                   <  2:4996633/93‑1 (MQ=255)
                                                                      c‑gcTGGTGCGCTGGCTACGCGG‑TCATTTGCGCATGACCGACACCATCGACAACGAGCATTTCTTTGTGCGTAAGAATGGATTGCTGTACGCca                   >  2:4497818/1‑93 (MQ=255)
                                                                      c‑gcTGGTGCGCTGGCTACGCGG‑TCATTTGCGCATGACCGACACCATCGACAACGAGCATTTCTTTGTGCGTAAGAATGGATTGCTGTACGCca                   <  1:1162209/93‑1 (MQ=255)
                                                                      c‑gcTGGTGCGCTGGCTACGCGG‑TCATTTGCGCATGACCGACACCATCGACAACGAGCATTTCTTTGTGCGTAAGAATGGATTGCTGTACGCca                   >  1:457696/1‑93 (MQ=255)
                                                                      c‑gcTGGTGCGCTGGCTACGCGG‑TCATTTGCGCATGACCGACACCATCGACAACGAGCATTTCTTTGTGCGTAAGAATGGATTGCTGTACGCca                   >  2:5859851/1‑93 (MQ=255)
                                                                          tGGTGCGCTGGCTACGCGG‑TCATTTGCGCATGACCGACACCATCGACAACGAGCATTTCTTTGTGCGTAAGAATGGATTGCTGTACGCCacac                <  1:2893836/93‑1 (MQ=255)
                                                                             ggcgcTGGCTACGCGG‑TCATTTGCGCATGACCGACACCATCGACAACGAGCATTTCTTTGTGCGTAAGAATGGATTGCTGTACGCCACACCGc             <  1:2533347/92‑1 (MQ=255)
                                                                                gcTGGCTACGCGG‑TCATTTGCGCATGACCGACACCATCGACAACGAGCATTTCTTTGTGCGTAAGAATGGATTGCTGTACGCCACACCGCTGa          >  1:2742349/1‑93 (MQ=255)
                                                                                gcTGGCTACGCGG‑TCATTTGCGCATGACCGACACCATCGACAACGAGCATTTCTTTGTGCGTAAGAATGGATTGCTGTACGCCACACCGCTGa          >  1:5244862/1‑93 (MQ=255)
                                                                                gcTGGCTACGCGG‑TCATTTGCGCATGACCGACACCATCGACAACGAGCATTTCTTTGTGCGTAAGAATGGATTGCTGTACGCCACACCGCTGa          >  1:5428796/1‑93 (MQ=255)
                                                                                 cTGGCTACGCGG‑TCATTTGCGCATGACCGACACCATCGACAACGAGCATTTCTTTGTGCGTAAGAATGGATTGCTGTACGCCACACCGCTGAt         >  1:5658095/1‑93 (MQ=255)
                                                                                  tGGCTACGCGG‑TCATTTGCGCATGACCGACACCATCGACAACGAGCATTTCTTTGTGCGTAAGAATGGATTGCTGTACGCCACACCGCTGATg        >  2:2532792/1‑93 (MQ=255)
                                                                                  tGGCTACGCGG‑TCATTTGCGCATGACCGACACCATCGACAACGAGCATTTCTTTGTGCGTAAGAATGGATTGCTGTACGCCACACCGCTGATg        >  2:1401743/1‑93 (MQ=255)
                                                                                   ggCTACGCGG‑TCATTTGCGCATGACCGACACCATCGACAACGAGCATTTCTTTGTGCGTAAGAATGGATTGCTGTACGCCACACCGCTGATGc       >  2:1541499/1‑93 (MQ=255)
                                                                                   ggCTACGCGG‑TCATTTGCGCATGACCGACACCATCGACAACGAGCATTTCTTTGTGCGTAAGAATGGATTGCTGTACGCCACACCGCTGATGc       >  2:79631/1‑93 (MQ=255)
                                                                                    gCTACGCGG‑TCATTTGCGCATGACCGACACCATCGACAACGAGCATTTCTTTGTGCGTAAGAATGGATTGCTGTACGCCACACCGCTGATGCt      <  2:5098984/93‑1 (MQ=255)
                                                                                    gCTACGCGG‑TCATTTGCGCATGACCGACACCATCGACAACGAGCATTTCTTTGTGCGTAAGAATGGATTGCTGTACGCCACACCGCTGATGCt      <  2:4683356/93‑1 (MQ=255)
                                                                                    gCTACGCGG‑TCATTTGCGCATGACCGACACCATCGACAACGAGCATTTCTTTGTGCGTAAGAATGGATTGCTGTACGCCACACCGCTGATGCt      <  2:653047/93‑1 (MQ=255)
                                                                                     cTACGCGG‑TCATTTGCGCATGACCGACACCATCGACAACGAGCATTTCTTTGTGCGTAAGAATGGATTGCTGTACGCCACACCGCTGATGCTg     <  1:1722962/93‑1 (MQ=255)
                                                                                     cTACGCGG‑TCATTTGCGCATGACCGACACCATCGACAACGAGCATTTCTTTGTGCGTAAGAATGGATTGCTGTACGCCACACCGCTGATGCTg     <  1:4737109/93‑1 (MQ=255)
                                                                                      tACGCGG‑TCATTTGCGCATGACCGACACCATCGACAACGAGCATTTCTTTGTGCGTAAGAATGGATTGCTGTACGCCACACCGCTGATGCTgg    >  1:3206268/1‑93 (MQ=255)
                                                                                      tACGCGG‑TCATTTGCGCATGACCGACACCATCGACAACGAGCATTTCTTTGTGCGTAAGAATGGATTGCTGTACGCCACACCGCTGATGCTgg    <  1:5986395/93‑1 (MQ=255)
                                                                                       aCGCGG‑TCATTTGCGCATGACCGACACCATCGACAACGAGCATTTCTTTGTGCGTAAGAATGGATTGCTGTACGCCACACCGCTGATGCTgga   >  2:1105195/1‑92 (MQ=255)
                                                                                       aCGCGG‑TCATTTGCGCATGACCGACACCATCGACAACGAGCATTTCTTTGTGCGTAAGAATGGATTGCTGTACGCCACACCGCTGATGCTGGt   >  2:2454796/1‑93 (MQ=255)
                                                                                             |                                                                                        
GCCTTCCTGCTGTTTACCGGCGTGAAGATGGCGCTCGCCCATGAAGATGAATCAGGCATTGGCGACAAG‑C‑GCTGGTGCGCTGGCTACGCGGTTCATTTGCGCATGACCGACACCATCGACAACGAGCATTTCTTTGTGCGTAAGAATGGATTGCTGTACGCCACACCGCTGATGCTGGTA  >  NC_000913/3236666‑3236845

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 19 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
GCCTTCCTGCTGTTTACCGGCGTGAAGATGGCGCTCGCCCATGAAGATGAATCAGGCATTGGCGACAAG‑C‑GCTGGTGCGCTGGCTACGCGGTTCATTTGCGCATGACCGACACCATCGACAACGAGCATTTCTTTGTGCGTAAGAATGGATTGCTGTACGCCACACCGCTGATGCTGGTACTGATT  >  NC_000913/3236666‑3236851
                                                                                              |                                                                                             
GCCTTCCTGCTGTTTACCGGCGTGAAGATGGCGCTCGCCCATGAAGATGAATCAGGCATTGGCGACAAGCC‑GCTGGTGCGCTGGCTACGCGGTC                                                                                               >  SRR4302537.5930820/1‑94 (MQ=60)
GCCTTCCTGCTGTTTACCGGCGTGAAGATGGCGCTCGCCCATGAAGATGAATCAGGCATTGGCGACAAGCC‑GCCGGTGCGCTGGCTACGCGGTC                                                                                               >  SRR4302537.6438564/1‑94 (MQ=60)
  CTTCCTGCTGTTTACCGGCGTGAAGATGGCGCTCGCCCATGAAGATGAATCAGGCATTGGCGACAAGCC‑GCTGGTGCGCTGGCTACGCGG‑TCAT                                                                                            >  SRR4302537.985403/1‑94 (MQ=60)
   TTCCTGCTGTTTACCGGCGTGAAGATGGCGCTCGCCCATGAAGATGAATCAGGCATTGGCGACAAGCC‑GCTGGTGCGCTGGCTACGCGG‑TCATT                                                                                           <  SRR4302537.1880552/94‑1 (MQ=60)
   TTCCTGCTGTTTACCGGCGTGAAGATGGCGCTCGCCCATGAAGATGAATCAGGCATTGGCGACAAGCC‑GCTGGTGCGCTGGCTACGCGG‑TCATT                                                                                           <  SRR4302537.5945737/94‑1 (MQ=60)
     CCTGCTGTTTACCGGCGTGAAGATGGCGCTCGCCCATGAAGATGAATCAGGCATTGGCGACAAGCC‑GCTGGTGCGCTGGCTACGCGG‑TCATTTG                                                                                         <  SRR4302537.3296329/94‑1 (MQ=60)
     CCTGCTGTTTACCGGCGTGAAGATGGCGCTCGCCCATGAAGATGAATCAGGCATTGGCGACAAGCC‑GCTGGTGCGCTGGCTACGCGG‑TCATTTG                                                                                         >  SRR4302537.1074587/1‑94 (MQ=60)
     CCTGCTGTTTACCGGCGTGAAGATGGCGCTCGCCCATGAAGATGAATCAGGCATTGGCGACAAGCC‑GCTGGGGCGCTGGCTACGCGG‑TCATTTG                                                                                         >  SRR4302537.3218635/1‑94 (MQ=60)
      CTGCTGTTTACCGGCGTGAATATGGCGCTCGCCCATGAAGATGAATCAGGCATTGGCGACAAGCC‑GCTGGTGCGCTGGCTACGCGG‑TCATTTGC                                                                                        <  SRR4302537.1894890/94‑1 (MQ=60)
      CTGCTGTTTACCGGCGTGAAGATGGCGCTCGCCCATGAAGATGAATCAGGCATTGGCGACAAGCC‑GCTGGTGCGCTGGCTACGCGG‑TCATTTGC                                                                                        <  SRR4302537.1972087/94‑1 (MQ=60)
      CTGCTGTTTACCGGCGTGAAGATGGCGCTCGCCCATGAAGATGAATCAGGCATTGGCGACAAGCC‑GCTGGTGCGCTGGCTACGCGG‑TCATTTGC                                                                                        >  SRR4302537.4521582/1‑94 (MQ=60)
      CTGCTGTTTACCGGCGTGAAGATGGCGCTCGCCCATGAAGATGAATCAGGCATTGGCGACAAGCC‑GCTGGTGCGCTGGCTACGCGG‑TCATTTGC                                                                                        >  SRR4302537.4684591/1‑94 (MQ=60)
       TGTTGTTTCCCGGCGTCAAGATGGCGCTCGCCCATGAAGATGAATCAGCCATTGGCGACAAGCC‑GCTGGTGCGCTGGCTACGCGG‑TCATTTGCG                                                                                       <  SRR4302537.365822/94‑1 (MQ=60)
       TGCTGTTTACCGGCGTGAAGATGGCGCTCGCCCATGAAGATGAATCAGGCATTGGCGACAAGCC‑GCTGGTGCGCTGGCTACGCGG‑TCATTTGCG                                                                                       >  SRR4302537.5325485/1‑94 (MQ=60)
        GCTGTTTACCGGCGTGAAGATGGCGCTCGCCCATGAAGATGAATCAGGCATTGGCGACAAGCC‑GCTGGTGCGCTGGCTACGCGG‑TCATTTGCGC                                                                                      >  SRR4302537.1470737/1‑94 (MQ=60)
         CTGTTTACCGGCGTGAAGATGGCGCTCGCCCATGAAGATGAATCAGGCATTGGCGACAAGCC‑GCTGGTGCGCTGGCTACGCGG‑TCATTTGCGCA                                                                                     >  SRR4302537.6515435/1‑94 (MQ=60)
          TGTTTACCGGCGTGAAGATGGCGCTCGCCCATGAAGATGAATCAGTCATTGGCGACAAGCC‑GCTGGTGCGCTGGCTACGCGG‑TCATTTGCGCAT                                                                                    <  SRR4302537.2231882/94‑1 (MQ=60)
          TGTTTACCGGCGTGAAGATGGCGCTCGCCCATGAAGATGAATCAGGCATTGGCGACAAGCC‑GCTGGTGCGCTGGCTACGCGG‑TCATTTGCGCAT                                                                                    <  SRR4302537.4910661/94‑1 (MQ=60)
          TGTTTACCGGCGTGAAGATGGCGCTCGCCCATGAAGATGAATCAGGCATTGGCGACAAGCC‑GCTGGTGCGCTGGCTACGCGG‑TCATTTGCGCAT                                                                                    <  SRR4302537.4247190/94‑1 (MQ=60)
          TGTTTACCGGCGTGAAGATGGCGCTCGCCCATGAAGATGAATCAGGCATTGGCGACAAGCC‑GCTGGTGCGCTGGCTACGCGG‑TCATTTGCGCAT                                                                                    <  SRR4302537.3155588/94‑1 (MQ=60)
          TGTTTACCGGCGTGAAGATGGCGCTCGCCCATGAAGATGAATCAGGCATTGGCGACAAGCC‑GCTGGTGCGCTGGCTACGCGG‑TCATTTGCGCAT                                                                                    <  SRR4302537.2381672/94‑1 (MQ=60)
          TGTTTACCGGCGTGAAGATGGCGCTCGCCCATGAAGATGAATCAGGCATTGGCGACAAGCC‑GCTGGTGCGCTGGCTACGCGG‑TCATTTGCGCAT                                                                                    <  SRR4302537.683724/94‑1 (MQ=60)
          TGTTTACCGGCGTGAAGATGGCGCTCGCCCATGAAGATGAATCAGGCATTGGCGACAAGAC‑GCTGGTGCGCTGGCTACGCGG‑TCATTTGCGCAT                                                                                    <  SRR4302537.629298/94‑1 (MQ=60)
            TTTACCGGCGTGAAGATGGCGCTCGCCCATGAAGATGAATCAGGCATTGGCGACAAGCC‑GCTGGTGCGCTGGCTACGCGG‑TCCTTTGCGCATGA                                                                                  >  SRR4302537.3327306/1‑94 (MQ=60)
               TCCGGCGTGAAGATGGCGCTCGCCCATGAAGATGAATCAGGCATTGGCGACAAGCC‑GCTGGTGCGCTGGCTACGCGG‑TCATTTGCGCATGACCG                                                                               <  SRR4302537.4332888/94‑1 (MQ=60)
               ACCGGCGTGAAGATGGCGCTCGCCCATGAAGATGAATCAGGCATTGGCGACAAGCC‑GCTGGTGCGCTGGCTACGCGG‑TCATTTGCGCATGACCG                                                                               >  SRR4302537.378518/1‑94 (MQ=60)
                CCGGCGTGAAGATGGCGCTCGCCCATGAAGATGAATCAGGCATTGGCGACAAGCC‑GCTGGTGCGCTGGCTACGCGG‑TCATTTGCGCATGACCGA                                                                              <  SRR4302537.4559719/94‑1 (MQ=60)
                 CGGCGTGAAGATGGCGCTCGCCCATGAAGATGAATCAGGCATTGGCGACAAGCC‑GCTGGTGCGCTGGCTACGCGG‑TCATTTGCGCATGACCGAC                                                                             >  SRR4302537.1056865/1‑94 (MQ=60)
                 CGGCGTGAAGATGGCGCTCGCCCATGAAGATGAATCAGGCATTGGCGACAAGCC‑GCTGGTGCGCTGGCTACGCGG‑TCATTTGCGCATGACCGAC                                                                             >  SRR4302537.1258046/1‑94 (MQ=60)
                  GGCGTGAAGATGGCGCTCGCCCATGAAGATGAATCAGGCATTGGCGACAAGCC‑GCTGGTGCGCTGGCTACGCGG‑TCATTTGCGCATGACCGACA                                                                            <  SRR4302537.989794/94‑1 (MQ=60)
                  GGCGTGAAGATGGCGCTCGCCCATGAAGATGAATCAGGCATTGGCGACAAGCC‑GCTGGTGCGCTGGCTACGCGG‑TCATTTGCGCATGACCGACA                                                                            >  SRR4302537.1388752/1‑94 (MQ=60)
                     GTGAATAGGGCGCTCGCCCATGAAGATGAATCAGGCATTGGCGACAAGCC‑GCTGGTGCGCTGGCTACGCGG‑TCATTTGCGCATGACCTACACCA                                                                         <  SRR4302537.5884104/94‑1 (MQ=60)
                     GTGAAGATGGCGCTCGCCCATGAAGATGAATCAGGCATTGGCGACAAGCC‑GCTGGTGCGCTGGCTACGCGG‑TCATTTGCGCATGACCGACACCA                                                                         <  SRR4302537.4232896/94‑1 (MQ=60)
                     GTGAAGATGGCGCTCGCCCATGAAGATGAATCAGGCATTGGCGACAAGCC‑GCTGGTGCGCTGGCTACGCGG‑TCATTTGCGCATGACCGACACCA                                                                         <  SRR4302537.5846974/94‑1 (MQ=60)
                      TGAAGATGGCGCTCGCCCATGAAGATGAATCAGGCATTGGCGACAAGCC‑GCTGGTGCGCTGGCTACGCGG‑TCATTTGCGCATGACCGACACCAT                                                                        >  SRR4302537.6177585/1‑94 (MQ=60)
                      TGAAGATGGCGCTCGCCCATGAAGATGAATCAGGCATTGGCGACAAGCC‑GCTGGTGCGCTGGCTACGCGG‑TCATTTGCGCATGACCGACACCAT                                                                        >  SRR4302537.6029377/1‑94 (MQ=60)
                       GAAGATGGCGCTCGCCCATGAAGATGAATCAGGCATTGGCGACAAGCC‑GCTGGTGCGCTGGCTACGCGG‑TCATTTGCGCATGACCGACACCATC                                                                       <  SRR4302537.2598436/94‑1 (MQ=60)
                       GAAGATGGCGCTCGCCCATGAAGATGAATCAGGCATTGGCGACAAGCC‑GCTGGTGCGCTGGCTACGCGG‑TCATTTGCGCATGACCGACACCATC                                                                       >  SRR4302537.4155308/1‑94 (MQ=60)
                       GAAGATGGCGCTCGCCCATGAAGATGAAGCAGGCATTGGCGACAAGCC‑GCTGGTGCGCTGGCTACGCGG‑TCATTTGCGCATGACCGACACCATC                                                                       <  SRR4302537.4440264/94‑1 (MQ=60)
                        AAGATGGCGCTCGCCCATGAAGATGAATCAGGCATTGGCGACAAGCC‑GCTGGTGCGCTGGCTACGCGG‑TCATTTGCGCATGACCGACACCATCG                                                                      <  SRR4302537.530935/94‑1 (MQ=60)
                        AAGATGGCGCTCGCCCATGAAGATGAATCAGGCATTGGCGACAAGCC‑GCTGGTGCGCTGGCTACGCGG‑TCATTTGCGCATGACCGACACCATCG                                                                      >  SRR4302537.82706/1‑94 (MQ=60)
                        AAGATGGCGCTCGCCCATGAAGATGAATCAGGCATTGGCGACAAGCC‑GCTGGTGCGCTGGCTACGCGG‑TCATTTGCGCATGACCGACACCATCG                                                                      >  SRR4302537.3928435/1‑94 (MQ=60)
                        AAGATGGCGCTCGCCCATGAAGATGAATCAGGCATTGGCGACAAGCC‑GCTGGTGCGCTGGCTACGCGG‑TCATTTGCGCATGACCGACACCATCG                                                                      >  SRR4302537.6033489/1‑94 (MQ=60)
                        AAGATGGCGCTCGCCCATGAAGATGAATCAGGCATTGGCGACAAGCC‑GCTGGTGCGCTGGCTACGCGG‑TCATTTGCGCATGACCGACACCATCG                                                                      >  SRR4302537.724465/1‑94 (MQ=60)
                        AAGATGGCGCTCGCCCATGAAGATGAATCAGGCATTGGCGACAAGCC‑GCTGGTGCGCTGGCTACGCGG‑TCATTTGCGCATGACCGACACCATCG                                                                      <  SRR4302537.2503299/94‑1 (MQ=60)
                         AGATGGCGCTCGCCCATGAAGATGAATCAGGCATTGGCGACAAGCC‑GCTGGTGCGCTGGCTACGCGG‑TCATTTGCGCATGACCGACACCATCGA                                                                     >  SRR4302537.5707363/1‑94 (MQ=60)
                         AGATGGCGCTCGCCCATGAAGATGAATCAGGCATTGGCGACAAG‑CAGCTGGTGCGCTGGCTACGCGG‑CCCTTTGAGCATGACCGACACCATCGC                                                                     >  SRR4302537.1822911/1‑94 (MQ=60)
                           ATGGCGCTCGCCCATGAAGATGAATCAGGCATTGGCGACAAGCC‑GCTGGTGCGCTGGCTACGCGG‑TCATTTGCGCATGACCGACACCATCGACA                                                                   >  SRR4302537.2883330/1‑94 (MQ=60)
                           ATGGCGCTCGCCCATGAAGATGAATCAGGCATTGGCGACAAGCC‑GCTGGTGCGCTGGCTACGCGG‑TCATTTGCGCATGACCGACACCATCGACA                                                                   >  SRR4302537.2881615/1‑94 (MQ=60)
                           ATGGCGCTCGCCCATGAAGATGAATCAGGCATTGGCGACAAGCC‑GCTGGTGCGCTGGCTACGCGG‑TCATTTGCGCATGACCGACACCATCGACA                                                                   >  SRR4302537.6289235/1‑94 (MQ=60)
                           ATGGCGCTCGCCCATGAAGATGAATCAGGCATTGGCGACAAGCC‑GCTGGTGCGCTGGCTACGCGG‑TCATTTGCGCATGACCGACACCATCGACA                                                                   >  SRR4302537.2969770/1‑94 (MQ=60)
                              GCGCTCGCCCATGAAGATGAATCAGGCATTGGCGACAAGCC‑GCTGGTGCGCTGGCTACGCGG‑TCATTTGCGCATGACCGACACCATCGACAACG                                                                <  SRR4302537.1055633/94‑1 (MQ=60)
                              GCGCTCGCCCATGAAGATGAATCAGGCATTGGCGACAAGCC‑GCTGGTGCGCTGGCTACGCGG‑TCATTTGCGCATGACCGACACCATCGACAACG                                                                >  SRR4302537.5915447/1‑94 (MQ=60)
                               GGCTCGCCCATGAAGATGAATCAGGCATTGGCGACAAGCC‑GCTGGTGCGCTGGCTACGCGG‑TCATTTGCGCATGACCGACACCATCGACAACGA                                                               <  SRR4302537.4921603/94‑1 (MQ=60)
                               CGCTCGCCCATGAAGATGAATCAGGCATTGGCGACAAGCC‑GCTGGTGCGCTGGCTACGCGG‑TCATTTGCGCATGACCGACACCATCGACAACGA                                                               >  SRR4302537.5966437/1‑94 (MQ=60)
                               CGCTCGCCCATGAAGATGAATCAGGCATTGGCGACAAGCC‑GCTGGTGCGCTGGCTACGCGG‑TCATTTGCGCATGACCGACACCATCGACAACGA                                                               >  SRR4302537.2636492/1‑94 (MQ=60)
                               CGCTCGCCCATGAAGATGAATCAGGCATTGGCGACAAGCC‑GCTGGTGCGCTGGCTACGCGG‑TCATTTGCGCATGACCGACACCATCGACAACGA                                                               >  SRR4302537.2601270/1‑94 (MQ=60)
                               CGCTCGCCCATGAAGATGAATCAGGCATTGGCGACAAGCC‑GCTGGTGCGCTGGCTACGCGG‑TCATTTGCGCATGACCGACACCATCGACAACGA                                                               <  SRR4302537.3003315/94‑1 (MQ=60)
                               CGCTCGCCCATGAAGATGAATCAGGCATTGGCGACAAGCC‑GCTGGTGCGCTGGCTACGCGG‑TCATTTGCGCATGACCGACACCATCGACAACGA                                                               <  SRR4302537.5451371/94‑1 (MQ=60)
                                GCTCGCCCATGAAGATGAATCAGGCATTGGCGACAAGCC‑GCTGGTGCGCTGGCTACGCGG‑TCATTTGCGCATGACCGACACCATCGACAACGAG                                                              <  SRR4302537.1788952/94‑1 (MQ=60)
                                GCTCGCCCATGAAGATGAATCAGGCATTGGCGACAAGCC‑GCTGGTGCGATGGCTACGCGG‑TCATTTGCGCATGACCGACACCATCGACAACGAG                                                              <  SRR4302537.82381/94‑1 (MQ=60)
                                GCTCGCCCATGAAGATAAATCAGGCATGGGCGACAAGCC‑GCTGGTGCGCTGGCTACGCGG‑TCATTTGCGCATGACCGACACCATCGACAACGAG                                                              <  SRR4302537.4895750/94‑1 (MQ=60)
                                CCTCGCCCATGAAGATGAATCAGGCATTGGCGACAAGCC‑GCTGGTGCGCTGGCTACGCGG‑TCATTTGCGCATGACCGACACCATCGACAACGAG                                                              <  SRR4302537.3986560/94‑1 (MQ=60)
                                 CTCGCCCATGAAGATGAATCAGGCATTGGCGACAAGCC‑GCTGGTGCGCTGGCTACGCGG‑TCATTTGCGCATGACCGACACCATCGACAACGAGC                                                             <  SRR4302537.1051932/94‑1 (MQ=60)
                                 CTCGCCCATGAAGATGAATCAGGCATTGGCGACAAGCC‑GCTGGTGCGCTGGCTACGCGG‑TCATTTGCGCATGACCGACACCATCGACAACGAGC                                                             >  SRR4302537.5565707/1‑94 (MQ=60)
                                  TCGCCCATGAAGATGAATCAGGCATTGGCGACAAGCC‑GCTGGTGCGCTGGCTACGCGG‑TCATTTGCGCATGACCGACACCATCGACAACGAGCA                                                            <  SRR4302537.5550550/94‑1 (MQ=60)
                                     CCCATGAAGATGAATCAGGCATTGGCGACAAGCC‑GCTGGTGCGCTGGCTACGCGG‑TCATTTGCGCATGACCGACACCATCGACAACGAGCATTT                                                         <  SRR4302537.4633915/94‑1 (MQ=60)
                                     CCCATGAAGATGAATCAGGCATTGGCGACAAGCC‑GCTGGTGCGCTGGCTACGCGG‑TCATTTGCGCATGACCGACACCATCGACAACGAGCATTT                                                         >  SRR4302537.6225164/1‑94 (MQ=60)
                                     CCCATGAAGATGAATCAGGCATTGGCGACAAGCC‑GCTGGTGCGCTGGCTACGCGG‑TCATTTGCGCATGACCGACACCATCGACAACGAGCATTT                                                         >  SRR4302537.2933277/1‑94 (MQ=60)
                                      CCATGAAGATGAATCAGGCATTGGCGACAAGCC‑GCTGGTGCGCTGGCTACGCGG‑TCATTTGCGCATGACCGACACCATCGACAACGAGCATTTC                                                        >  SRR4302537.3333875/1‑94 (MQ=60)
                                       cgtgaaaataaatcatgcagtggcgataagcC‑GCTGGTGCGCTGGCTACGCGG‑TCATTTGCGCATGACCGACACCATCGACAACGAGCATTTCT                                                       <  SRR4302537.1091697/63‑1 (MQ=60)
                                       CATGAAGATGAATCAGGCATTGGCGACAAGCC‑GCTGGTGCGCTGGCTACGCGG‑TCATTTGCGCATGACCGACACCATCGACAACGAGCATTTCT                                                       >  SRR4302537.4373682/1‑94 (MQ=60)
                                       CATGAAGATGAATCAGGCATTGGCGACAAGCC‑GCTGGTGCGCTGGCTACGCGG‑TCATTTGCGCATGACCGACACCATCGACAACGAGCATTTCT                                                       <  SRR4302537.4362108/94‑1 (MQ=60)
                                        ATGAAGATGAATCAGGCATTGGCGACAAGCC‑GCTGGTGCGCTGGCTACGCGG‑TCATTTGCGCATGACCGACACCATCGACAACGAGCATTTCTT                                                      >  SRR4302537.2777512/1‑94 (MQ=60)
                                        ATGAAGATGAATCAGGCATTGGCGACAAGCC‑GCTGGTGCGCTGGCTACGCGG‑TCATTTGCGCATGACCGACACCATCGACAACGAGCATTTCTT                                                      <  SRR4302537.3943972/94‑1 (MQ=60)
                                        ATGAAGATGAATCAGGCATTGGCGACAAGCC‑GCTGGTGCGCTGGCTACGCGG‑TCATTTGAGCATGACCGACACCATCGACAACGAGCATCTCTT                                                      >  SRR4302537.2878342/1‑94 (MQ=60)
                                         TGAAGATGAATCAGGCATTGGCGACAAGCC‑GCTGGTGCGCTGGCTACGCGG‑TCATTTGCGCATGACCGACACCATCGACAACGAGCATTTCTTT                                                     <  SRR4302537.334413/94‑1 (MQ=60)
                                          GAAGATGAATCAGGCATTGGGGACAAGCC‑GCTGGTGCGCTGGCTACGCGG‑TCATTTGCGCATGACCGACACCATCGACAACGAGCATTTCTTTG                                                    <  SRR4302537.1101792/94‑1 (MQ=60)
                                           AAGATGAATCAGGCATTGGCGACAAGCC‑GCTGGTGCGCTGGCTACGCGG‑TCATTTGCGCATGACCGACACCATCGACAACGAGCATTTCTTTGT                                                   <  SRR4302537.182731/94‑1 (MQ=60)
                                            AGATGAATCAGGCATTGGCGACAAGCC‑GCTGGTGCGCTGGCTACGCGG‑TCATTTGCGCATGACCGACACCATCGACAACGAGCATTTCTTTGTG                                                  <  SRR4302537.3248804/94‑1 (MQ=60)
                                            AGATGAATCAGGCATTGGCGACAAGCC‑GCTGGTGCGCTGGCTACGCGG‑TCATTTGCGCATGACCGACACCATCGACAACGAGCATTTCTTTGTG                                                  >  SRR4302537.157034/1‑94 (MQ=60)
                                             TATGAATCAGGCATGGCCAACAAGCC‑GCTGGTGCGCTGGCTACGCGG‑TCATTTGCGCATGACCGACACCATCGACAACGAGCATTTCTTTGTGC                                                 <  SRR4302537.4862404/94‑1 (MQ=60)
                                             GATGAATCAGGCATTGGCGACAAGCC‑GCTGGTGCGCTGGCTACGCGG‑TCATTTGCGCATGACCGACACCATCGACAACGAGCATTTCTTTGTGC                                                 >  SRR4302537.6379884/1‑94 (MQ=60)
                                             GATGAATCAGGCATTGGCGACAAGCC‑GCTGGTGCGCTGGCTACGCGG‑GCATTTGCGCATGACCGACACCATCGACAACGAGCATTTCTTTGTGG                                                 >  SRR4302537.3451043/1‑94 (MQ=60)
                                              ATGAATCAGGCATTGGCGACAAGCC‑GCTGGTGCGCTGGCTACGCGG‑TCATTTGCGCATGACCGACACCATCGACAACGAGCATTTCTTTGTGCG                                                <  SRR4302537.1223801/94‑1 (MQ=60)
                                              ATGAATCAGGAATGGGCGACAAGCC‑GCTGGTGCGCTGGCTACGCGG‑TCATTTGCGCATGACCGACACCATCGACAACGAGCATTTCTTTGTGCG                                                <  SRR4302537.1003346/94‑1 (MQ=60)
                                                GAATCAGGCATTGGCGACAAGCC‑GCTGGTGCGCTGGCTACGCGG‑TCATTTGCGCATGACCGACACCATCGACAACGAGCATTTCTTTGTGCGTA                                              >  SRR4302537.5868654/1‑94 (MQ=60)
                                                 AATCAGGCATTGGCGACAAGCC‑GCTGGTGCGCTGGCTACGCGG‑TCATTTGCGCATGACCGACACCATCGACAACGAGCATTTCTTTGTGCGTAA                                             >  SRR4302537.286265/1‑94 (MQ=60)
                                                   TCAGGCATTGGCGAAAAGCC‑GCTGGTGCGCTGGCTACGCGG‑TCATTTGCGGATGACCGACACCATCGACAACGAGCATTTCTTTGTGCGTAAGA                                           <  SRR4302537.4579214/94‑1 (MQ=60)
                                                     AGGCATTGGCGACAAGCC‑GCTGGTGCGCTGGCTACGCGG‑TCATTTGCGCATGACCGACACCATCGACAACGAGCATTTCTTTGTGCGTAAGAAT                                         >  SRR4302537.6332691/1‑94 (MQ=60)
                                                       gcgttgcgaacaagcC‑GCTGGTGCGCTGGCTACGCGG‑TCATTTGCGCATGACCGACACCATCGACAACGAGCATTTCTTTGTGCGTAAGAATGG                                       <  SRR4302537.3680700/79‑1 (MQ=60)
                                                       GCATTGGCGACAAGCC‑GCTGGTGCGCTGGCTACGCGG‑TCATTTGCGCATGACCGACACCATCGACAACGAGCATTTCTTTGTGCGTAAGAATGG                                       >  SRR4302537.2920319/1‑94 (MQ=60)
                                                       GCATGGGCGACAAGCC‑GCTGGTGCGCTGGCTACGCGG‑TCATTTGCGCATGACCGACACCATCGACAACGAGCATTTCTTTGTGCGTAAGAATGG                                       <  SRR4302537.6088904/94‑1 (MQ=60)
                                                       GCATGGGCGACAAGCC‑GCTGGTGCGCTGGCTACGCGG‑TCATTTGCGCATGACCGACACCATCGACAACGAGCATTTCTTTGTGCGTAAGAATGG                                       <  SRR4302537.114407/94‑1 (MQ=60)
                                                        CATTGGCGACAAGCC‑GCTGGTGCGCTGGCTACGCTG‑TCATTTGCGCATGACCGACACCATCGACAACGAGCATTTCTTTGTGCGTAAGAATGGA                                      <  SRR4302537.4426517/94‑1 (MQ=60)
                                                        CATTGGCGACAAGCC‑GCTGGTGCGCTGGCTACGCGG‑TCATTTGCGCATGACCGACACCATCGACAACGAGCATTTCTTTGTGCGTAAGAATGGA                                      >  SRR4302537.5627758/1‑94 (MQ=60)
                                                         ATGGGCGACAAGCC‑GCTGGTGCGCTGGCTACGCGG‑TCATTTGCGCATGACCGACACCATCGACAACGAGCATTTCTTTGTGCGTAAGAATGGAT                                     <  SRR4302537.2288891/94‑1 (MQ=60)
                                                          TTGGCGACAAGCC‑GCTGGTGCGCTGGCTACGCGG‑TCATTTGCGCATGACCGACACCATCGACAACGAGCATTTCTTTGTGCGTAAGAATGGATT                                    <  SRR4302537.4034809/94‑1 (MQ=60)
                                                           TGGCGACAAGCC‑GCTGGTGCGCTGGCTACGCGG‑TCATTTGCGCATGACCGACACCATCGACAACGAGCATTTCTTTGTGCGTAAGAATGGATTG                                   >  SRR4302537.4659601/1‑94 (MQ=60)
                                                           TGGCGACAAGCC‑GCTGGTGCGCTGGCTACGCGG‑TCATTTGCGCATGACCGACACCATCGACAACGAGCATTTCTTTGTGCGTAAGAATGGATTG                                   >  SRR4302537.1977626/1‑94 (MQ=60)
                                                           TGGCGACAAGCC‑GCTGGTGCGCTGGCTACGCGG‑TCATTTGCGCATGACCGACACCATCGACAACGAGCATTTCTTTGTGCGTAAGAATGGATTG                                   <  SRR4302537.5143373/94‑1 (MQ=60)
                                                            GGCGACAAGCC‑GCTGGTGCGCTGGCTACGCGG‑TCATTTGCGCATGACCGACACCATCGACAACGAGCATTTCTTTGTGCGTAAGAATGGATTGC                                  <  SRR4302537.864472/94‑1 (MQ=60)
                                                             GCGACAAGCC‑GCTGGTGCGCTGGCTACGCGG‑TCATTTGCGCATGACCGACACCATCGACAACGAGCATTTCTTTGTGCGTAAGAATGGATTGCT                                 <  SRR4302537.2950026/94‑1 (MQ=60)
                                                               GACAAGCC‑GCTGGTGCGCTGGCTACGCGG‑TCATTTGCGCATGACCGACACCATCGACAACGAGCATTTCTTTGTGCGTAAGAATGGATTGCTGT                               >  SRR4302537.2267930/1‑94 (MQ=60)
                                                                ACAAGCC‑GCTGGTGCGCTGGCTACGCGG‑TCATTTGCGCATGACCGACACCATCGACAACGAGCATTTCTTTGTGCGTAAGAATGGATTGCTGTA                              <  SRR4302537.2520276/94‑1 (MQ=60)
                                                                ACAAGCC‑GCTGGTGCGCTGGCTACGCGG‑TCATTTGCGCATGACCGACACCATCGACAACGAGCATTTCTTTGTGCGTAAGAATGGATTGCTGTA                              <  SRR4302537.2012401/94‑1 (MQ=60)
                                                                 CAAGCC‑GCTGGTGCGCTGGCTACGCGG‑TCATTTGCGCATGACCGACACCATCGACAACGAGCATTTCTTTGTGCGTAAGAATGGATTGCTGTAC                             <  SRR4302537.4349323/94‑1 (MQ=60)
                                                                 CAAGCC‑GCTGGTGCGCTGGCTACGCGG‑TCATTTGCGCATGACCGACACCATCGACAACGAGCATTTCTTTGTGCGTAAGAATGGATTGCTGTAC                             <  SRR4302537.5344236/94‑1 (MQ=60)
                                                                  AAGCC‑GCTGGTGCGCTGGCTACGCGG‑TCATTTGCGCATGACCGACACCATCGACAACGAGCATTTCTTTGTGCGTAAGAATGGATTGCTGTACG                            <  SRR4302537.5319264/94‑1 (MQ=60)
                                                                   agcC‑GCTGGTGCGCTGGCTACGCGG‑TCATTTGCGCATGACCGACACCATCGACAACGAGCATTTCTTTGTGCGTAAGAATGGATTGCTGTACGC                           >  SRR4302537.4138747/4‑94 (MQ=60)
                                                                   agcC‑GCTGGTGCGCTGGCTACGCGG‑TCATTTGCGCATGACCGACACCATCGACAACGAGCATTTCTTTGTGCGTAAGAATGGATTGCTGTACGC                           >  SRR4302537.4636286/4‑94 (MQ=60)
                                                                    C‑C‑GCTGGTGCGCTGGCTACGCGG‑TCATTTGCGCATGACCGACACCATCGACAACGAGCATTTCTTTGTGCGTAAGAATGGATTGCTGTACGCCA                         >  SRR4302537.460852/1‑94 (MQ=60)
                                                                      C‑GCTGGTGCGCTGGCTACGCGG‑TCATTTGCGCATGACCGACACCATCGACAACGAGCATTTCTTTGTGCGTAAGAATGGATTGCTGTACGCCAC                        <  SRR4302537.1170293/94‑1 (MQ=60)
                                                                      C‑GCTGGTGCGCTGGCTACGCGG‑TCATTTGCGCATGACCGACACCATCGACAACGAGCATTTCTTTGTGCGTAAGAATGGATTGCTGTACGCCAC                        <  SRR4302537.1808388/94‑1 (MQ=60)
                                                                      C‑GCTGGTGCGCTGGCTACGCGG‑TCATTTGCGCATGACCGACACCATCGACAACGAGCATTTCTTTGTGCGTAAGAATGGATTGCTGTACGCCAC                        <  SRR4302537.2819460/94‑1 (MQ=60)
                                                                        GCTGGTGCGCTGGCTACGCGG‑TCATTTGCGCATGACCGACACCATCGACAACGAGCATTTCTTTGTGCGTAAGAATGGATTGCTGTACGCCACA                       >  SRR4302537.1678962/1‑94 (MQ=60)
                                                                        GCTGGTGCGCTGGCTACGCGG‑TCATTTGCGCATGACCGACACCATCGACAACGAGCATTTCTTTGTGCGTAAGAATGGATTGCTGTACGCCACA                       >  SRR4302537.192291/1‑94 (MQ=60)
                                                                        GCTGGTGCGCTGGCTACGCGG‑TCATTTGCGCATGACCGACACCATCGACAACGAGCATTTCTTTGTGCGTAAGAATGGATTGCTGTACGCCACA                       >  SRR4302537.2293250/1‑94 (MQ=60)
                                                                        GCTGGTGCGCTGGCTACGCGG‑TCATTTGCGCATGACCGACACCATCGACAACGAGCATTTCTTTGTGCGTAAGAATGGAGTGCTGTACGCCACA                       >  SRR4302537.2744872/1‑94 (MQ=60)
                                                                          TGGTGCGCTGGCTACGCGG‑TCATTTGCGCATGACCGACACCATCGACAACGAGCATTTCTTTGTGCGTAAGAATGGATTGCTGTACGCCACACC                     <  SRR4302537.3649122/94‑1 (MQ=60)
                                                                          TGGTGCGCTGGCTACGCGG‑TCATTTGCGCATGACCGACACCATCGACAACGAGCATTTCTTTGTGCGTAAGAATGGATTGCTGTACGCCACACC                     <  SRR4302537.2914149/94‑1 (MQ=60)
                                                                            GTGCGCTGGCTACGCGG‑TCATTTGCGCATGACCGACACCATCGACAACGAGCATTTCTTTGTGCGTAAGAATGGATTGCTGTACGCCACACCGC                   <  SRR4302537.2925577/94‑1 (MQ=60)
                                                                             TGCGCTGGCTACGCGG‑TCATTTGCGCATGACCGACACCATCGACAACGAGCATTTCTTTGTGCGTAAGAATGGATTGCTGTACGCCACACCGCT                  <  SRR4302537.984708/94‑1 (MQ=60)
                                                                             TGCGCTGGCTACGCGG‑TCATTTGCGCATGACCGACACCATCGACAACGAGCATTTCTTTGTGCGTAAGAATGGATTGCTGTACGCCACACCGCT                  <  SRR4302537.3040250/94‑1 (MQ=60)
                                                                             GGCGCTGGCTACGCGG‑TCATTTGCGCATGACCGACACCATCGACAACGAGCATTTCTTTGTGCGTAAGAATGGATTGCTGTACGCCACACCGCT                  <  SRR4302537.2551125/94‑1 (MQ=60)
                                                                              GCGCTGGCTACGCGG‑TCATTTGCGCATGACCGACACCATCGACAACGAGCATTTCTTTGTGCGTAAGAATGGATTGCTGTACGCCACACCGCTG                 <  SRR4302537.5934973/94‑1 (MQ=60)
                                                                               CGCTGGCTACGCGG‑TCATTTGCGCATGACCGACACCATCGACAACGAGCATTTCTTTGTGCGTAAGAATGGATTGCTGTACGCCACACCGCTGA                >  SRR4302537.5465812/1‑94 (MQ=60)
                                                                               CGCTGGCTACGCGG‑TCATTTGCGCATGACCGACACCATCGACAACGAGCATTTCTTTGTGCGTAAGAATGGATTGCTGTACGCCACACCGCTGA                >  SRR4302537.2761481/1‑94 (MQ=60)
                                                                               CGCTGGCTACGCGG‑TCATTTGCGCATGACCGACACCATCGACAACGAGCATTTCTTTGTGCGTAAGAATGGATTGCTGTACGCCACACCGCTGA                >  SRR4302537.5280953/1‑94 (MQ=60)
                                                                                GCTGGCTACGCGG‑TCATTTGCGCATGACCGACACCATCGACAACGAGCATTTCTTTGTGCGTAAGAATGGATTGCTGTACGCCACACCGCTGAT               <  SRR4302537.5131795/94‑1 (MQ=60)
                                                                                GCTGGCTACGCGG‑TCATTTGCGCATGACCGACACCATCGACAACGAGCATTTCTTTGTGCGTAAGAATGGATTGCTGTACGCCACACCGCTGAT               <  SRR4302537.4808273/94‑1 (MQ=60)
                                                                                GCTGGCTACGCGG‑TCATTTGCGCATGACCGACACCATCGACAACGAGCATTTCTTTGTGCGTAAGAATGGATTGCTGTACGCCACACCGCTGAT               >  SRR4302537.5696822/1‑94 (MQ=60)
                                                                                 CTGGCTACGCGG‑TCATTTGCGCATGACCGACACCATCGACAACGAGCATTTCTTTGTGCGTAAGAATGGATTGCTGTACGCCACACCGCTGATG              <  SRR4302537.4327989/94‑1 (MQ=60)
                                                                                   GGCTACGCGG‑TCATTTGCGCATGACCGACACCATCGACAACGAGCATTTCTTTGTGCGTAAGAATGGATTGCTGTACGCCACACCGCTGATGCT            >  SRR4302537.5542736/1‑94 (MQ=60)
                                                                                     CTACGCGG‑TCATTTGCGCATGACCGACACCATCGACAACGAGCATTTCTTTGTGCGTAAGAATGGATTGCTGTACGCCACACCGCTGATGCTGG          >  SRR4302537.1459089/1‑94 (MQ=60)
                                                                                     CTACGCGG‑TCATTTGCGCATGACCGACACCATCGACAACGAGCATTTCTTTGTGCGTAAGAATGGATTGCTGTACGCCACACCGCTGATGCTGG          <  SRR4302537.5119342/94‑1 (MQ=60)
                                                                                     CTACGCGG‑TCATTTGCGCATGACCGACACCATCGACAACGAGCATTTCTTTGTGCGTAAGAATGGATTGCTGTACGCCACACCGCTGATGCTGG          >  SRR4302537.3228809/1‑94 (MQ=60)
                                                                                     CTACGCGG‑TCATTTGCGCATGACCGACACCATCGACAACGAGCATTTCTTTGTGCGTAAGAATGGATTGCTGTACGCCACACCGCTGATGCTGG          <  SRR4302537.4769855/94‑1 (MQ=60)
                                                                                     CTACGCGG‑TCATTTGCGCATGACCGACACCATCGACAACGAGCATTTCTTTGTGCGTAAGAATGGATTGCTGTACGCCACACCGCTGATGCTGG          <  SRR4302537.5315355/94‑1 (MQ=60)
                                                                                     CTACGCGG‑TCATTTGCGCATGACCGACACCATCGACAACGAGCATTTCTTTGTGCGTAAGAATGGATTGCTGTACGCCACACCGCTGATGCTGG          >  SRR4302537.6214691/1‑94 (MQ=60)
                                                                                     CTACGCGG‑TCATTTGCGCATGACCGACACCATCGACAACGAGCATTTCTTTGTGCGTAAGAATGGATTGCTGTACGCCACACCGCTGATGCTGG          <  SRR4302537.1735004/94‑1 (MQ=60)
                                                                                      TACGCGG‑TCATTTGCGCATGACCGACACCATCGACAACGAGCATTTCTTTGTGCGTAAGAATGGATTGCTGTACGCCACACCGCTGATGCTGGT         <  SRR4302537.6027478/94‑1 (MQ=60)
                                                                                          CGG‑TCATTTGCGCATGACCGACACCATCGACAACGAGCATTTCTTTGTGCGTAAGAATGGATTGCTGTACGCCACACCGCTGCTGCTGGTACTG     >  SRR4302537.5622318/1‑94 (MQ=60)
                                                                                            GGTCATTTGCGCATGACCGACACCATCGACAACGAGCATTTCTTTGTGCGTAAGAATGGATTGCTGTACGCCACACCGCTGATGCTGGTACTGA    <  SRR4302537.247525/94‑1 (MQ=60)
                                                                                             GTCATTTGCGCATGACCGACACCATCGACAACGAGCATTTCTTTGTGCGTAAGAATGGATTGCTGTACGCCACACCGCTGATGCTGGTACTGAT   <  SRR4302537.4504979/94‑1 (MQ=60)
                                                                                             GTCATTTGCGCATGACCGACACCATCGACAACGAGCATTTCTTTGTGCGTAAGAATGGATTGCTGTACGCCACACCGCTGATGCTGGTACTGAT   >  SRR4302537.3101572/1‑94 (MQ=60)
                                                                                             GTCATTTGCGCATGACCGACACCATCGACAACGAGCATTTCTTTGTGCGTAAGAATGGATTGCTGTACGCCACACCGCTGATGCTGGTACTGAT   <  SRR4302537.3815020/94‑1 (MQ=60)
                                                                                              TCATTTGCGCATGACCGACACCATCGACAACGAGCATTTCTTTGTGCGTAAGAATGGATTGCTGTACGCCACACCGCTGATGCTGGTACTGATT  >  SRR4302537.6018244/1‑94 (MQ=60)
                                                                                              TCATTTGCGCATGACCGACACCATCGACAACGAGCATTTCTTTGTGCGTAAGAATGGATTGCTGTACGCCACACCGCTGATGCTGGTACTGATT  >  SRR4302537.1333584/1‑94 (MQ=60)
                                                                                              |                                                                                             
GCCTTCCTGCTGTTTACCGGCGTGAAGATGGCGCTCGCCCATGAAGATGAATCAGGCATTGGCGACAAG‑C‑GCTGGTGCGCTGGCTACGCGGTTCATTTGCGCATGACCGACACCATCGACAACGAGCATTTCTTTGTGCGTAAGAATGGATTGCTGTACGCCACACCGCTGATGCTGGTACTGATT  >  NC_000913/3236666‑3236851

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 2 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑