Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A1 F1 I2 R1	882	254.4	12957588	98.0%	12698436	93.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,299,656	T→C	Y177Y (TAT→TAC)	yhbU →	putative collagenase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,299,656	0	T	C	96.0%	714.4 / 12.6	223	Y177Y (TAT→TAC)	yhbU	putative collagenase
Reads supporting (aligned to +/- strand): ref base T (5/4); new base C (112/101); total (118/105)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.47e-01

Only 100 of 228 total aligned reads displayed.

CAGGTGAAACAACTGGCACGGGTCACACCTGTACCACTGGAAGTCTTTGCTTTCGGCAGCCTGTGCATTATGTCGGAAGGTCGTTGCTATCTGTCGTCGTATCTGACGGGTGAGTCGCCCAACACCATAGGCGCGTGTTCTCCGGCCCGTTTCGTGCGCTGGCAACAAACGCCGCAGGG  >  NC_000913/3299567‑3299745

 aGGTGAAACAACTGGCACGGGTCACACCTGTACCACTGGAAGTCTTTGCTTTCGGCAGCCTGTGCATTATGTCGGAAGGTCGTTGCTACCTgt                                                                                       >  1:3290894/1‑93 (MQ=255)

 aGGTGAAACAACTGGCACGGGTCACACCTGTACCACTGGAAGTCTTTGCTTTCGGCAGCCTGTGCATTATGTCGGAAGGTCGTTGCTACCTgt                                                                                       <  1:4523302/93‑1 (MQ=255)

  ggTGAAACAACTGGCACGGGTCACACCTGTACCACTGGAAGTCTTTGCTTTCGGCAGCCTGTGCATTATGTCGGAAGGTCGTTGCTATCTgtc                                                                                      <  2:4394027/93‑1 (MQ=255)

   gTGAAACAACTGGCACGGGTCACACCTGTACCACTGGAAGTCTTTGCTTTCGGCAGCCTGTGCATTATGTCGGAAGGTCGTTGCTACCTgtcg                                                                                     <  1:554106/93‑1 (MQ=255)

    tGAAACAACTGGCACGGGTCACACCTGTACCACTGGAAGTCTTTGCTTTCGGCAGCCTGTGCATTATGTCGGAAGGTCGTTGCTACCTgtcgt                                                                                    <  1:1111356/93‑1 (MQ=255)

    tGAAACAACTGGCACGGGTCACACCTGTACCACTGGAAGTCTTTGCTTTCGGCAGCCTGTGCATTATGTCGGAAGGTCGTTGCTACCTgtcgc                                                                                    >  2:2863076/1‑92 (MQ=255)

     gAAACAACTGGCACGGGTCACACCTGTACCACTGGAAGTCTTTGCTTTCGGCAGCCTGTGCATTATGTCGGAAGGTCGTTGCTACCTgtcgtc                                                                                   <  2:5711076/93‑1 (MQ=255)

       aacaacTGGCACGGGTCACACCTGTACCACTGGAAGTCTTTGCTTTCGGCAGCCTGTGCATTATGTCGGAAGGTCGTTGCTACCTGTcgtcgt                                                                                 <  2:1771918/93‑1 (MQ=255)

       aacaacTGGCACGGGTCACACCTGTACCACTGGAAGTCTTTGCTTTCGGCAGCCTGTGCATTATGTCGGAAGGTCGTTGCTACCTGTcgtcgt                                                                                 >  1:240565/1‑93 (MQ=255)

        acaacTGGCACGGGTCACACCTGTACCACTGGAAGTCTTTGCTTTCGGCAGCCTGTGCATTATGTCGGAAGGTCGTTGCTACCTGTCGTCGTa                                                                                <  2:4654637/93‑1 (MQ=255)

        acaacTCGCACGAGGCACACCTGTACCACTGGAAGTCTTTGCTTCCGGCAGGCTGTGCATTATGTCGGAAGGTCGTTGCTACCTGTCGTCGTa                                                                                <  2:1113272/93‑1 (MQ=255)

         caacTGGCACGGGTCACACCTGTACCACTGGAAGTCTTTGCTTTCGGCAGCCTGTGCATTATGTCGGAAGGTCGTTGCTACCTGTCGTCGTAt                                                                               <  2:5454322/93‑1 (MQ=255)

          aacTGGCACGGGTCACACCTGTACCACTGGAAGTCTTTGCTTTCGGCAGCCTGTGCATTATGTCGGAAGGTCGTTGCTACCTGTCGTCGTATc                                                                              <  1:5916665/93‑1 (MQ=255)

          aacTGGCACGGGTCACACCTGTACCACTGGAAGTCTTTGCTTTCGGCAGCCTGTGCATTATGTCGGAAGGTCGTTGCTACCTGTCGTCGTATc                                                                              <  2:5909482/93‑1 (MQ=255)

           acTGGCACGGGTCACACCTGTACCACTGGAAGTCTTTGCTTTCGGCAGCCTGTGCATTATGTCGGAAGGTCGTTGCTACCTGTCGTCGTATCt                                                                             >  1:6242320/1‑93 (MQ=255)

           acTGGCACGGGTCACACCTGTACCACTGGAAGTCTTTGCTTTCGGCAGCCTGTGCATTATGTCGGAAGGTCGTTGCTACCTGTCGTCGTATCt                                                                             <  2:5840067/93‑1 (MQ=255)

           acTGGCACGGGTCACACCTGTACCACTGGAAGTCTTTGCTTTCGGCAGCCTGTGCATTATGTCGGAAGGTCGTTGCTACCTGTCGTCGTATCt                                                                             <  2:808186/93‑1 (MQ=255)

           acTGGCACGGGTCACACCTGTACCACTGGAAGTCTTTGCTTTCGGCAGCCTGTGCATTATGTCGGAAGGTCGTTGCTACCTGTCGTCGTATCt                                                                             >  1:1116845/1‑93 (MQ=255)

           acTGGCACGGGTCACACCTGTACCACTGGAAGTCTTTGCTTTCGGCAGCCTGTGCATTATGTCGGAAGGTCGATGCTACCTGTCGTCGTATCt                                                                             >  1:239287/1‑93 (MQ=255)

               gCACGGGTCACACCTGTACCACTGGAAGTCTTTGCTTTCGGCAGCCTGTGCATTATGTCGGAAGGTCGTTGCTACCTGTCGTCGTATCTGACg                                                                         <  2:6317272/93‑1 (MQ=255)

                  cGGGTCACACCTGTACCACTGGAAGTCTTTGCTTTCGGCAGCCTGTGCATTATGTCGGAAGGTCGTTGCTACCTGTCGTCGTATCTGACGGGt                                                                      >  2:2246751/1‑93 (MQ=255)

                   gggTCACACCTGTACCACTGGAAGTCTTTGCTTTCGGCAGCCTGTGCATTATGTCGGAAGGTCGTTGCTACCTGTCGTCGTATCTGACGGGTg                                                                     <  2:2174296/93‑1 (MQ=255)

                      tCACACCTGTACCACTGGAAGTCTTTGCTTTCGGCAGCCTGTGCATTATGTCGGAAGGTCGTTGCTATCTGTCGTCGTATCTGACGGGTGAGt                                                                  <  2:4202082/93‑1 (MQ=255)

                      tCACACCTGTACCACTGGAAGTCTTTGCTTTCGGCAGCCTGTGCATTATGTCGGAAGGTCGTTGCTACCTGTCGTCGTATCTGACGGGTGAGt                                                                  >  1:3140793/1‑93 (MQ=255)

                         cacCTGTACCACTGGAAGTCTTTGCTTTCGGCAGCCTGTGCATTATGTCGGAAGGTCGTTGCTACCTGTCGTCGTATCTGACGGGTGAGTCGc                                                               >  1:2801186/1‑93 (MQ=255)

                         cacCTGTACCACTGGAAGTCTTTGCTTTCGGCAGCCTGTGCATTATGTCGGAAGGTCGTTGCTACCTGTCGTCGTATCTGACGGGTGAGTCGc                                                               >  1:5655180/1‑93 (MQ=255)

                           ccTGTACCACTGGAAGTCTTTGCTTTCGGCAGCCTGTGCATTATGTCGGAAGGTCGTTGCTACCTGTCGTCGTATCTGACGGGTGAGTCGccc                                                             >  2:5993682/1‑93 (MQ=255)

                            cTGTACCACTGGAAGTCTTTGCTTTCGGCAGCCTGTGCATTATGTCGGAAGGTCGTTGCTATCTGTCGTCGTATCTGACGGGTGAGTCGCCCa                                                            >  2:3014629/1‑93 (MQ=255)

                             tGTACCACTGGAAGTCTTTGCTTTCGGCAGCCTGTGCATTATGTCGGAAGGTCGTTGCTACCTGTCGTCGTATCTGACGGGTGAGTCGCCCaa                                                           <  2:512465/93‑1 (MQ=255)

                              gTACCACTGGAAGTCTTTGCTTTCGGCAGCCTGTGCATTATGTCGGAAGGTCGTTGCTACCTGTCGTCGTATCTGACGGGTGAGTCGCCCAac                                                          <  1:2142045/93‑1 (MQ=255)

                              gTACCACTGGAAGTCTTTGCTTTCGGCAGCCTGTGCATTATGTCGGAAGGTCGTTGCTACCTGTCGTCGTATCTGACGGGTGAGTCGCCCAac                                                          >  1:1904474/1‑93 (MQ=255)

                              gTACCACTGGAAGTCTTTGCTTTCGGCAGCCTGTGCATTATGTCGGAAGGTCGTTGCTACCTGTCGTCGTATCTGACGGGTGAGTCGCCCAac                                                          <  2:1272907/93‑1 (MQ=255)

                                aCCACTGTAAGTCTTTGCTTTCGGCAGCCTGTGCATTATGTCGGAAGGTCGTTGCTACCTGTCGTCGTATCTGACGGGTGAGTCGCCCAacac                                                        <  1:2501494/93‑1 (MQ=255)

                                aCCACTGGAAGTCTTTGCTTTCGGCAGCCTGTGCATTATGTCGGAAGGTCGTTGCTACCTGTCGTCGTATCTGACGGGTGAGTCGCCCAacac                                                        <  1:6348439/93‑1 (MQ=255)

                                 ccACTGGAAGTCTTTGCTTTCGGCAGCCTGTGCATTATGTCGGAAGGTCGTTGCTACCTGTCGTCGTATCTGACGGGTGAGTCGCCCAACAcc                                                       >  1:2833108/1‑93 (MQ=255)

                                  cACTGGAAGTCTTTGCTTTCGGCAGCCTGTGCATTATGTCGGAAGGTCGTTGCTACCTGTCGTCGTATCTGACGGGTGAGTCGCCCAACACCa                                                      <  2:602502/93‑1 (MQ=255)

                                  cACTGGAAGTCTTTGCTTTCGGAAGCCTGTGCATTATGTCGGAAGGTCGTTGCTACCTGTCGTCGTATCTGACGGGGGAGTCGCCCAACACCa                                                      >  2:2904240/1‑93 (MQ=255)

                                   aCTGGAAGTCTTTGCTTTCGGCAGCCTGTGCATTATGTCGGAAGGTCGTTGCTACCTGTCGTCGTATCTGACGGGTGAGTCGCCCAACACCAt                                                     >  1:1494973/1‑93 (MQ=255)

                                   aCTGGAAGTCTTTGCTTTCGGCAGCCTGTGCATTATGTCGGAAGGTCGTTGCTACCTGTCGTCGTATCTGACGGGTGAGTCGCCCAACACCAt                                                     >  2:246730/1‑93 (MQ=255)

                                     tGGAAGTCTTTGCTTTCGGCAGCCTGTGCATTATGTCGGAAGGTCGTTGCTATCTGTCGTCGTATCTGACGGGTGAGTCGCCCAACACCATAg                                                   >  2:3410177/1‑93 (MQ=255)

                                     tGGAAGTCTTTGCTTTCGGCAGCCTGTGCATTATGTCGGAAGGTCGTTGCTACCTGTCGTCGTATCTGACGGGTGAGTCGCCCAACACCATAg                                                   <  1:4836047/93‑1 (MQ=255)

                                     tGGAAGTCTTTGCTTTCGGCAGCCTGTGCATTATGTCGGAAGGTCGTTGCTACCTGTCGTCGTATCTGACGGGTGAGTCGCCCAACACCATAg                                                   >  2:4905907/1‑93 (MQ=255)

                                      ggAAGTCTTTGCTTTCGGCAGCCTGTGCATTATGTCGGAAGGTCGTTGCTACCTGTCGTCGTATCTGACGGGTGAGTCGCCCAACACCATaag                                                  >  2:3210039/1‑91 (MQ=255)

                                      ggAAGTCTTTGCTTTCGGCAGCCTGTGCATTATGTCGGAAGGTCGTTGCTACCTGTCGTCGTATCTGACGGGTGAGTCGCCCAACACCATAgg                                                  >  1:5220266/1‑93 (MQ=255)

                                      ggAAGTCTTTGCTTTCGGCAGCCTGTGCATTATGTCGGAAGGTCGTTGCTACCTGTCGTCGTATCTGACGGGTGAGTCGCCCAACACCATAgg                                                  >  1:865343/1‑93 (MQ=255)

                                       gAAGTCTTTGCTTTCGGCAGCCTGTGCATTATGTCGGAAGGTCGTTGCTACCTGTCGTCGTATCTGACGGGTGAGTCGCCCAACACCATAGgc                                                 <  2:3292216/93‑1 (MQ=255)

                                       gAAGTCTTTGCTTTCGGCAGCCTGTGCATTATGTCGGAAGGTCGTTGCTACCTGTCGTCGTATCTGACGGGTGAGTCGCCCAACACCATAGgc                                                 >  2:1982064/1‑93 (MQ=255)

                                         aGTCTTTGCTTTCGGCAGCCTGTGCATTATGTCGGAAGGTCGTTGCTACCTGTCGTCGTATCTGACGGGTGAGTCGCCCAACACCATAGgcgc                                               <  1:3668723/93‑1 (MQ=255)

                                          gTCTTTGCTTTCGGCAGCCTGTGCATTATGTCGGAAGGTCGTTGCTACCTGTCGTCGTATCTGACGGGTGAGTCGCCCAACACCATAGGcgcg                                              <  1:2718624/93‑1 (MQ=255)

                                           tCTTTGCTTTCGGCAGCCTGTGCATTATGTCGGAAGGTCGTTGCTACCTGTCGTCGTATCTGACGGGTGAGTCGCCCAACACCATAGGCGCgt                                             <  1:3379356/93‑1 (MQ=255)

                                            cTTTGCTTTCCGGAGCCGGTGCATTATGTCGGAAGGTCGTTGCTACCTGTCGTCGTATCTGACGGGTGAGTCGCCCAACACCATAGGCGCgtg                                            <  2:3954084/93‑1 (MQ=255)

                                            cTTTGCTTTAGGCAGCCTGTGCATTATGTCGGAAGGTCGTTGCTACCTGTCGTCGTATCTGACGGGTGAGTCGCCCAACACCATAGGCGCgtg                                            <  1:3267370/93‑1 (MQ=255)

                                             tttGCTTTCGGCAGCCTGTGCATTATGTCGGAAGGTCGTTGCTACCTGTCGTCGTATCTGACGGGTGAGTCGCCCAACACCATAGGCGCgtgt                                           >  1:1694262/1‑93 (MQ=255)

                                             tttGCTTTCGGCAGCCTGTGCATTATGTCGGAAGGTCGTTGCTACCTGTCGTCGTATCTGACGGGTGAGTCGCCCAACACCATAGGCGCgtgt                                           <  1:1146555/93‑1 (MQ=255)

                                              ttGCTTTCGGCAGCCTGTGCATTATGTCGGAAGGTCGTTGCTACCTGTCGTCGTATCTGACGGGTGAGTCGCCCAACACCATAGGCGCGTGtt                                          >  2:3267921/1‑93 (MQ=255)

                                              ttGCTTTCGGCAGCCTGTGCATTATGTCGGAAGGTCGTTGCTACCTGTCGTCGTATCTGACGGGTGAGTCGCCCAACACCATAGGCGCGTGtt                                          <  1:4983222/93‑1 (MQ=255)

                                               tGCTTTCGGCAGCCTGTGCATTATGTCGGAAGGTCGTTGCTACCTGTCGTCGTATCTGACGGGTGAGTCGCCCAACACCATAGGCGCGTGTtc                                         >  1:6076697/1‑93 (MQ=255)

                                                gCTTTCGGCAGCCTGTGCATTATGTCGGAAGGTCGTTGCTACCTGTCGTCGTATCTGACGGGTGAGTCGCCCAACACCATAGGCGCGTGTtct                                        >  1:5357143/1‑93 (MQ=255)

                                                gCTTTCGGCAGCCTGTGCATTATGTCGGAAGGTCGTTGCTACCTGTCGTCGTATCTGACGGGTGAGTCGCCCAACACCATAGGCGCGTGTtct                                        >  2:4539495/1‑93 (MQ=255)

                                                gCTTTCGGCAGCCTGTGCATTATGTCGGAAGGTCGTTGCTACCTGTCGTCGTATCTGACGGGTGAGTCGCCCAACACCATAGGCGCGTGTtct                                        >  1:2587187/1‑93 (MQ=255)

                                                gCTTTCGGCAGCCTGTGCATTATGTCGGAAGGTCGTTGCTACCTGTCGTCGTATCTGACGGGTGAGTCGCCCAACACCATAGGCGCGTGTtct                                        >  1:6415171/1‑93 (MQ=255)

                                                 cTTTCGGCAGCCTGTGCATTATGTCGGAAGGTCGTTGCTACCTGTCGTCGTATCTGACGGGTGAGTCGCCCAACACCATAGGCGCGTGTtctc                                       >  2:4434716/1‑93 (MQ=255)

                                                 cTTTCGGCAGCCTGTGCATTATGTCGGAAGGTCGTTGCTACCTGTCGTCGTATCTGACGGGTGAGTCGCCCAACACCATAGGCGCGTGTtctc                                       >  2:4731473/1‑93 (MQ=255)

                                                 cTTTCGGCAGCCTGTGCATTATGTCGGAAGGTCGTTGCTACCTGTCGTCGTATCTGACGGGTGAGTCGCCCAACACCATAGGCGCGTGTtctc                                       >  2:737654/1‑93 (MQ=255)

                                                      gtcAGCCTGTGCATTATGTCGGAAGGTCGTTGCTACCTGTCGTCGTATCTGACGGGTGAGTCGCCCAACACCATAGGCGCGTGTTCTCCGGcc                                  >  1:4927289/3‑93 (MQ=255)

                                                          gCCTGTGCATTATGTCGGAAGGTCGTTGCTACCTGTCGTCGTATCTGACGGGTGAGTCGCCCAACACCATAGGCGCGTGTTCTCCGGCCCGtt                              <  1:6155208/93‑1 (MQ=255)

                                                           ccTGTGCATTATGTCGGAAGGTCGTTGCTACCTGTCGTCGTATCTGACGGGTGAGTCGCCCAACACCATAGGCGCGTGTTCTCCGGCCCGttt                             >  1:4494160/1‑93 (MQ=255)

                                                            cTGTGCATTATGTCGGAAGGTCGTTGCTACCTGTCGTCGTATCTGACGGGTGAGTCGCCCAACACCATAGGCGCGTGTTCTCCGGCCCGTTTc                            <  2:2494274/93‑1 (MQ=255)

                                                            cTGTGCATTATGTCGGAAGGTCGTTGCTACCTGTCGTCGTATCTGACGGGTGAGTCGCCCAACACCATAGGCGCGTGTTCTCCGGCCCGTTTc                            >  2:2343384/1‑93 (MQ=255)

                                                             tgtgCATTATGTCGGAAGGTCGTTGCTACCTGTCGTCGTATCTGACGGGTGAGTCGCCCAACAGCATAGGCGCGTGGTCTCCGGCCCGTTTCg                           >  1:3352699/1‑93 (MQ=255)

                                                             tgtgCATTATGTCGGAAGGTCGTTGCTACCTGTCGTCGTATCTGACGGGTGAGTCGCCCAACACCATAGGCGCGTGTTCTCCGGCCCGTTTCg                           >  1:4622534/1‑93 (MQ=255)

                                                             tgtgCATTATGTCGGAAGGTCGTTGCTACCTGTCGTCGTATCTGACGGGTGAGTCGCCCAACACCATAGGCGCGTGTTCTCCGGCCCGTTTCg                           >  1:4906751/1‑93 (MQ=255)

                                                             tgtgCATTATGTCGGAAGGTCGTTGCTACCTGTCGTCGTATCTGACGGGTGAGTCGCCCAACACCATAGGCGCGTGTTCTCCGGCCCGTTTCg                           >  2:5220034/1‑93 (MQ=255)

                                                             tgtgCATTATGTCGGAAGGTCGTTGCTACCTGTCGTCGTATCTGACGGGTGAGTCGCCCAACACCATAGGCGCGTGTTCTCCGCCCCGTTTct                           >  1:3489442/1‑92 (MQ=255)

                                                              gtgCATTATGTCGGAAGGTCGTTGCTACCTGTCGTCGTATCTGACGGGTGAGTCGCCCAACACCATAGACGCGTGTTCTCCGGCCCGTTTCGt                          >  1:5504027/1‑93 (MQ=255)

                                                                gCATTATGTCGGAAGGTCGTTGCTACCTGTCGTCGTATCTGACGGGTGAGTCGCCCAACACCATAGGCGCGTGTTCTCCGGCCCGTTTCGTgc                        <  1:5094403/93‑1 (MQ=255)

                                                                  aTTATGTCGGAAGGTCGTTGCTACCTGTCGTCGTATCTGACGGGTGAGTCGCCCAACACCATAGGCGCGTGTTCTCCGGCCCGTTTCGTgcgc                      <  1:4352868/93‑1 (MQ=255)

                                                                   ttATGTCGGAAGGTCGTTGCTACCTGTCGTCGTATCTGACGGGTGAGTCGCCCAACACCATAGGCGCGTGTTCTCCGGCCCGTTTCGTGCGCt                     >  1:1105013/1‑93 (MQ=255)

                                                                   ttATGTCGGAAGGTCGTTGCTACCTGTCGTCGTATCTGACGGGTGAGTCGCCCAACACCATAGGCGCGTGTTCTCCGGCCCGTTTCGTGCGCt                     >  1:1336982/1‑93 (MQ=255)

                                                                     aTGTCGGAAGGTCGTTGCTATCTGTCGTCGTATCTGACGGGTGAGTCGCCCAACACCATAGGCGCGTGTTCTCTGGCCCATTTCGTCCGCTgg                   >  2:2608251/1‑93 (MQ=255)

                                                                      tGTCGGAAGGTCGTTGCTACCTGTCGTCGTATCTGACGGGTGAGTCGCCCAACACCATAGGCGCGTGTTCTCCGGCCCGTTTCGTGCGCTGGc                  <  1:5229009/93‑1 (MQ=255)

                                                                       gTCGGAAGGTCGTTGCTACCTGTCGTCGTATCTGACGGGTGAGTCGCCCAACACCATAGGCGCGTGTTCTCCGGCCCGTTTCGTGCGCTGGca                 >  1:571019/1‑93 (MQ=255)

                                                                          ggAAGGTCGTTGCTACCTGTCGTCGTATCTGACGGGTGAGTCGCCCAACACCATAGGCGCGTGTTCTCCGGCCCGTTTCGTGCGCTGGcaaca              >  2:4098505/1‑93 (MQ=255)

                                                                           gAAGGTCGTTGCTATCTGTCGTCGTATCTGACGGGTGAGTCGCCCAACACCATAGGCGCGTGTTCTCCGGCCCGTTTCGTGCGCTGGcaacaa             >  2:5791019/1‑93 (MQ=255)

                                                                           gAAGGTCGTTGCTACCTGTCGTCGTATCTGACGGGTGAGTCGCCCAACACCATAGGCGCGTGTTCTCCGGCCCGTTTCGTGCGCTGGcaacaa             >  1:3568336/1‑93 (MQ=255)

                                                                           gAAGGTCGTTGCTACCTGTCGTCGTATCTGACGGGTGAGTCGCCCAACACCATAGGCGCGTGTTCTCCGGCCCGTTTCGTGCGCTGGcaacaa             >  1:3716627/1‑93 (MQ=255)

                                                                            aaGGTCGTTGCTATCTGTCGTCGTATCTGACGGGTGAGTCGCCCAACACCATAGGCGCGTGTTCTCCGGCCCGTTTCGTGCGCTGGCAACaaa            <  1:3801279/93‑1 (MQ=255)

                                                                            aaGGTCGTTGCTACCTGTCGTCGTATCTGACGGGTGAGTCGCCCAACACCATAGGCGCGTGTTCTCCGGCCCGTTTCGTGCGCTGGCAACaaa            >  2:4851664/1‑93 (MQ=255)

                                                                              ggTTGTTGCTACCTGTCGTCGTATCTGACGGGTGAGTCGCCCAACACCATAGGCGCGTGTTCTCCGGCCCGGTTCGTGCGCTGGCAACAAAcg          >  1:2236857/1‑93 (MQ=255)

                                                                               gTCGTTGCTACCTGTCGTCGTATCTGACGGGTGAGTCGCCCAACACCATAGGCGCGTGTTCTCCGGCCCGTTTCGTGCGCTGGCAACAAAcgc         <  1:3960903/93‑1 (MQ=255)

                                                                                tCGTTGCTACCTGTCGTCGTATCTGACGGGTGAGTCGCCCAACACCATAGGCGCGTGTTCTCCGGCCCGTTTCGTGCGCTGGCAACAAAcgcc        <  1:4135818/93‑1 (MQ=255)

                                                                                tCGTTGCTACCTGTCGTCGTATCTGACGGGTGAGTCGCCCAACACCATAGGCGCGTGTTCTCCGGCCCGTTTCGTGCGCTGGCAACAAAcgcc        >  2:1686082/1‑93 (MQ=255)

                                                                                tCGTTGCTACCTGTCGTCGTATCTGACGGGTGAGTCGCCCAACACCATAGGCGCGTGTTCTCCGGCCCGTTTCGTGCGCTGGCAACAAAcgcc        <  2:5768461/93‑1 (MQ=255)

                                                                                tCGTTGCTACCTGTCGTCGTATCTGACGGGTGAGTCGCCCAACACCATAGGCGCGTGTTCTCCGGCCCGTTTCGTGCGCTGGCAACAAAcgcc        <  2:2150223/93‑1 (MQ=255)

                                                                                  gTTGCTACCTGTCGTCGTATCTGACGGGTGAGTCGCCCAACACCATAGGCGCGTGTTCTCCGGCCCGTTTCGTGCGCTGGCAAAAAAcgccgc      >  1:2008358/1‑93 (MQ=255)

                                                                                   ttGCTACCTGTCGTCGTATCTGACGGGTGAGTCGCCCAACACCATAGGCGCGTGTTCTCCGGCCCGTTTCGTGCGCTGGCAACAAACGCCGCa     >  2:3645967/1‑93 (MQ=255)

                                                                                    tGCTACCTGTCGTCGTATCTGACGGGTGAGTCGCCCAACACCATAGGCGCGTGTTCTCCGGCCCGTTTCGTGCGCTGGCAACAAACGCCGCAg    >  1:4406393/1‑93 (MQ=255)

                                                                                     gCTACCTGTCGTCGTATCTGACGGGTGAGTCGCCCAACACCATAGGCGCGTGTTCTCCGGCCCGTTTCGTGCGCTGGCAACAAACGCCGCAgg   >  2:1857050/1‑93 (MQ=255)

                                                                                     gCTACCTGTCGTCGTATCTGACGGGTGAGTCGCCCAACACCATAGGCGCGTGTTCTCCGGCCCGTTTCGTGCGCTGGCAACAAACGCCGCAgg   <  2:4295474/93‑1 (MQ=255)

                                                                                      cTACCTGTCGTCGTATCTGACGGGTGAGTCGCCCAACACCATAGGCGTGTGTTCTGCGGTCCGTTTCGTGCGCTGGCAACAAAAGCCGCAcgg  >  1:5866908/1‑93 (MQ=255)

CAGGTGAAACAACTGGCACGGGTCACACCTGTACCACTGGAAGTCTTTGCTTTCGGCAGCCTGTGCATTATGTCGGAAGGTCGTTGCTATCTGTCGTCGTATCTGACGGGTGAGTCGCCCAACACCATAGGCGCGTGTTCTCCGGCCCGTTTCGTGCGCTGGCAACAAACGCCGCAGGG  >  NC_000913/3299567‑3299745

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 19 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

BRESEQ :: bam2aln output

TCATCAGGTGAAACAACTGGCACGGGTCACACCTGTACCACTGGAAGTCTTTGCTTTCGGCAGCCTGTGCATTATGTCGGAAGGTCGTTGCTATCTGTCGTCGTATCTGACGGGTGAGTCGCCCAACACCATAGGCGCGTGTTCTCCGGCCCGTTTCGTGCGCTGGCAACAAACGCCGCAGGGGCTG  >  NC_000913/3299563‑3299749

TCATCAGGTGAAACAACTGGCACGGGTCACACCTGTACCACTGGAAGTCTTTGCTTTCGGCAGCCTGTGCATTATGTCGGAAGGTCGTTGCTAT                                                                                               <  SRR4302537.6299612/94‑1 (MQ=60)

  ATCAGGTGAAACAACTGGCACGGGTCACACCTGTACCACTGGAAGTCTTTGCTTTCGGCAGCCTGTGCATTATGTCGGAAGGTCGTTGCTACCT                                                                                             <  SRR4302537.3314360/94‑1 (MQ=60)

    CAGGTGAAACAACTGGCACGGGTCACACCTGTACCACTGGAAGTCTTTGCTTTCGGCAGCCTGTGCATTATGTCGGAAGGTCGTTGCTACCTGT                                                                                           <  SRR4302537.290168/94‑1 (MQ=60)

    CAGGTGAAACAACTGGCACGGGTCACACCTGTACCACTGGAAGTCTTTGCTTTCGGCAGCCTGTGCATTATGTCGGAAGGTCGTTGCTACCTGT                                                                                           >  SRR4302537.3314148/1‑94 (MQ=60)

     AGGTGAAACAACTGGCACGGGTCACACCTGTACCACTGGAAGTCTTTGCTTTCGGCAGCCTGTGCATTATGTCGGAAGGTCGTTGCTACCTGTC                                                                                          <  SRR4302537.4554598/94‑1 (MQ=60)

       GTGAAACAACTGGCACGGGTCACACCTGTACCACTGGAAGTCTTTGCTTTCGGCAGCCTGTGCATTATGTCGGAAGGTCGTTGCTACCTGTCGT                                                                                        <  SRR4302537.557920/94‑1 (MQ=60)

        TGAAACACCTGGCACGGGTCACACCTGTACCACTGGAAGTCTTTGCTTTCGGCAGCCTGTGCATTATGTCGGAAGGTCGTTGCTACCTGTCGTC                                                                                       <  SRR4302537.2316861/94‑1 (MQ=60)

        TGAAACAACTGGCACGGGTCACACCTGTACCACTGGAAGTCTTTGCTTTCGGCAGCCTGTGCATTATGTCGGAAGGTCGTTGCTACCTGTCGTC                                                                                       <  SRR4302537.1119032/94‑1 (MQ=60)

          AAACAACTGGCACGGGTCACACCTGTACCACTGGAAGTCTTTGCTTTCGGCAGCCTGTGCATTATGTCGGAAGGTCGTTGCTACCTGTCGTCGT                                                                                     >  SRR4302537.242267/1‑94 (MQ=60)

            ACAACTGGCACGGGTCACACCTGTACCACTGGAAGTCTTTGCTTTCGGCATCCTGTGCATTATGTCGGAAGGTCGTTGCTACCTGTCGTCGTAT                                                                                   <  SRR4302537.4585837/94‑1 (MQ=60)

            ACAACTGGCACGGGTCACACCTGTACCACTGGAAGTCTTTGCTTTCGGCAGCCTGTGCATTATGTCGGAAGGTCGTTGCTACCTGTCGTCGTAT                                                                                   <  SRR4302537.2083877/94‑1 (MQ=60)

              AACTGGCACGGGTCACACCTGTACCACTGGAAGTCTTTGCTTTCGGCAGCCTGTGCATTATGTCGGAAGGTCGTTGCTACCTGTCGTCGTATCT                                                                                 >  SRR4302537.6285225/1‑94 (MQ=60)

              AACTGGCACGGGTCACACCTGTACCACTGGAAGTCTTTGCTTTCGGCAGCCTGTGCATTATGTCGGAAGGTCGTTGCTACCTGTCGTCGTATCT                                                                                 >  SRR4302537.1124571/1‑94 (MQ=60)

              AACTGGCACGGGTCACACCTGTACCACTGGAAGTCTTTGCTTTCGGCAGCCTGTGCATTATGTCGGAAGGTCGTTGCTACCTGTCGTCGTATCT                                                                                 <  SRR4302537.3058728/94‑1 (MQ=60)

              AACTGGCACGGGTCACACCTGTACCACTGGAAGTCTTTGCTTTCGGCAGCCTGTGCATTATGTCGGAAGGTCGTTGCTACCTGTCGTCGTATCT                                                                                 <  SRR4302537.5957262/94‑1 (MQ=60)

              AACTGGCACGGGTCACACCTGTACCACTGGAAGTCTTTGCTTTCGGCAGCCTGTGCATTATGTCGGAAGGTCGATGCTACCTGTCGTCGTATCT                                                                                 >  SRR4302537.240979/1‑94 (MQ=60)

               ACTGGCACGGGTCACACCTGTACCACTGGAAGTCTTTGCTTTCGGCAGCCTGTGCATTATGTCGGAAGGTCGTTGCTACCTGTCGTCGTATCTG                                                                                <  SRR4302537.2726469/94‑1 (MQ=60)

                  GGCACGGGTCACACCTGTACCACTGGAAGTCTTTGCTTTCGGCAGCCTGTGCATTATGTCGGAAGGTCGTTGCTACCTGTCGTCGTATCTGACG                                                                             <  SRR4302537.4880141/94‑1 (MQ=60)

                   GCACGGGTCACACCTGTACCACTGGAAGTCTTTGCTTTCGGCAGCCTGTGCATTATGTCGGAAGGTCGTTGCTACCTGTCGTCGTATCTGACGG                                                                            <  SRR4302537.6009985/94‑1 (MQ=60)

                     CCNGGTCACACCTGTACCACTNNAAGTCTTTGCTTTCGGCAGCCTGTGCATTATGTCGGAAGGTCGTTGCTACCTGTCGTCGTATCTGACGGGT                                                                          <  SRR4302537.3320755/94‑1 (MQ=60)

                     ACGGGTCACACCTGTACCACTGGAAGTCTTTGCTTTCGGCAGCCTGTGCATTATGTCGGAAGGTCGTTGCTACCTGTCGTCGTATCTGACGGGT                                                                          >  SRR4302537.3378645/1‑94 (MQ=60)

                     ACGGGTCACACCTGTACCACTGGAAGTCTTTGCTTTCGGCAGCCTGTGCATTATGTCGGAAGGTCGTTGCTACCTGTCGTCGTATCTGACGGGG                                                                          >  SRR4302537.1137575/1‑94 (MQ=60)

                      CGGGTCACACCTGTACCACTGGAAGTCTTTGCTTTCGGCAGCCTGTGCATTATGTCGGAAGGTCGTTGCTACCTGTCGTCGTATCTGACGGGTG                                                                         >  SRR4302537.4763418/1‑94 (MQ=60)

                        GGTCACACCTGTACCACTGGAAGTCTTTGCTTTCGGCAGCCTGTGCATTATGTCGGAAGGTCGTTGCTACCTGTCGTCGTATCTGACGGGTGAG                                                                       <  SRR4302537.1115427/94‑1 (MQ=60)

                         GTCACACCTGTACCACTGGAAGTCTTTGCTTTCGGCAGCCTGTGCATTATGTCGGAAGGTCGTTGCTACCTGTCGTCGTATCTGACGGGTGAGT                                                                      <  SRR4302537.6158284/94‑1 (MQ=60)

                         GTCACACCTGTACCACTGGAAGTCTTTGCTTTCGGCAGCCTGTGCATTATGTCGGAAGGTCGTTGCTACCTGTCGTCGTATCTGACGGGTGAGT                                                                      >  SRR4302537.3162835/1‑94 (MQ=60)

                         GTCACACCTGTACCACTGGAAGTCTTTGCTTTCGGCAGCCTGTGCATTATGTCGGAAGGTCGTTGCTACCTGTCGTCGTATCTGACGGGTGAGT                                                                      >  SRR4302537.4253662/1‑94 (MQ=60)

                         GTCACACCTGTACAACTGGAAGTCTTTGCTTTCGGCAGCCTGTGCATTATGTCGGAAGGTCGTTGCTACCTGTCGTCGTATCTGACGGGTGAGT                                                                      <  SRR4302537.1457331/94‑1 (MQ=60)

                          TCACACCTGTACCACTGGAAGTCTTTGCTTTCGGCAGCCTGTGCATTATGTCGGAAGGTCGTTGCTACCTGTCGTCGTATCTGACGGGTGAGTC                                                                     <  SRR4302537.5149873/94‑1 (MQ=60)

                           CACACCTGTACCACTGGAAGTCTTTGCTTTCGGCAGCCTGTGCATTATGTCGGAAGGTCGTTGCTACCTGTCGTCGTATCTGACGGGTGAGTCG                                                                    >  SRR4302537.5656961/1‑94 (MQ=60)

                           CACACCTGTACCACTGGAAGTCTTTGCTTTCGGCAGCCTGTGCATTATGTCGGAAGGTCGTTGCTACCTGTCGTCGTATCTGACGGGTGAGTCG                                                                    <  SRR4302537.4957534/94‑1 (MQ=60)

                           CACACCTGTACCACTGGAAGTCTTTGCTTTCGGCAGCCTGTGCATTATGTCGGAAGGTCGTTGCTACCTGTCGTCGTATCTGACGGGTGAGTCG                                                                    >  SRR4302537.508932/1‑94 (MQ=60)

                            ACACCTGTACCACTGGAAGTCTTTGCTTTCGGCAGCCTGTGCATTATGTCGGAAGGTCGTTGCTACCTGTCGTCGTATCTGACGGGTGAGTCGC                                                                   >  SRR4302537.2820769/1‑94 (MQ=60)

                            ACACCTGTACCACTGGAAGTCTTTGCTTTCGGCAGCCTGTGCATTATGTCGGAAGGTCGTTGCTACCTGTCGTCGTATCTGACGGGTGAGTCGC                                                                   >  SRR4302537.5693879/1‑94 (MQ=60)

                            ACACCTGTACCACTGGAAGTCTTTGCTTTCGGCAGCCTGTGCATTATGTCGGAAGGTCGTTGCTACCTGTCGTCGTATCTGACGGGTGAGTCGC                                                                   >  SRR4302537.3290380/1‑94 (MQ=60)

                            ACACCTGTACCACTGGAAGTCTTTGCTTTCGGCAGCCTGTGCATTATGTCGGAAGGTCGTTGCTACCTGTCGTCGTATCTGACGGGTGAGGCGC                                                                   >  SRR4302537.3919519/1‑94 (MQ=60)

                             CACCTGTACCACTGGAAGTCTTTGCTTTCGGCAGCCTGTGCATTATGTCGGAAGGTCGTTGCTACCTGTCGTCGTATCTGACGGGTGAGTCGCC                                                                  >  SRR4302537.2759091/1‑94 (MQ=60)

                              GCCTGTACCACTGGAAGTCTTTGCTTTCGGCAGCCTGTGCATTATGTCGGAAGGTCGTTGCTACCTGTCGTCGTATCTGACGGGTGAGTCGCCC                                                                 <  SRR4302537.194757/94‑1 (MQ=60)

                                 TGTACCACTGGAAGTCTTTGCTTTCGGCAGCCTGTGCATTATGTCGGAAGGTCGTTGCTACCTGTCGTCGTATCTGACGGGTGAGTCGCCCAAC                                                              >  SRR4302537.1959864/1‑94 (MQ=60)

                                 TGTACCACTGGAAGTCTTTGCTTTCGGCAGCCTGTGCATTATGTCGGAAGGTCGTTGCTACCTGTCGTCGTATCTGACGGGTGAGTCGCCCAAC                                                              >  SRR4302537.1917699/1‑94 (MQ=60)

                                  GTACCACTGGAAGTCTTTGCTTTCGGCAGCCTGTGCATTATGTCGGAAGGTCGTTGCTACCTGTCGTCGTATCTGACGGGTGAGTCGCCCAACA                                                             <  SRR4302537.5462083/94‑1 (MQ=60)

                                  GTACCACTGGAAGTCTTTGCTTTCGGCAGCCTGTGCATTATGTCGGAAGGTCGTTGCTACCTGTCGTCGTATCTGACGGGTGAGTCGCCCAACA                                                             <  SRR4302537.2157119/94‑1 (MQ=60)

                                   TACCACTGGAAGTCTTTGCTTTCGGCAGCCTGTGCATTATGTCGGAAGGTCGTTGCTACCTGTCGTCGTATCTGACGGGTGAGTCGCCCAACAC                                                            <  SRR4302537.377607/94‑1 (MQ=60)

                                    ACCACTGTAAGTCTTTGCTTTCGGCAGCCTGTGCATTATGTCGGAAGGTCGTTGCTACCTGTCGTCGTATCTGACGGGTGAGTCGCCCAACACC                                                           <  SRR4302537.2519091/94‑1 (MQ=60)

                                    ACCACTGGAAGTCTTTGCTTTCGGCAGCCTGTGCATTATGTCGGAAGGTCGTTGCTACCTGTCGTCGTATCTGACGGGTGAGTCGCCCAACACC                                                           >  SRR4302537.2852917/1‑94 (MQ=60)

                                    ACCACTGGAAGTCTTTGCTTTCGGCAGCCTGTGCATTATGTCGGAAGGTCGTTGCTACCTGTCGTCGTATCTGACGGGTGAGTCGCCCAACACC                                                           <  SRR4302537.6391794/94‑1 (MQ=60)

                                     CCACTGGAAGTCTTTGCTTTCGGCAGCCTGTGCATTATGTCGGAAGGTCGTTGCTACCTGTCGTCGTATCTGACGGGTGAGTCGCCCAACACCA                                                          >  SRR4302537.3587288/1‑94 (MQ=60)

                                      CACTGGAAGTCTTTGCTTTCGGCAGCCTGTGCATTATGTCGGAAGGTCGTTGCTACCTGTCGTCGTATCTGACGGGTGAGTCGCCCAACACCAT                                                         >  SRR4302537.1505393/1‑94 (MQ=60)

                                        CTGGAAGTCTTTGCTTTCGGCAGCCTGTGCATTATGTCGGAAGGTCGTTGCTACCTGTCGTCGTATCTGACGGGTGAGTCGCCCAACACCATAG                                                       <  SRR4302537.585539/94‑1 (MQ=60)

                                        CTGGAAGTCTTTGCTTTCGGCAGCCTGTGCATTATGTCGGAAGGTCGTTGCTACCTGTCGTCGTATCTGACGGGTGAGTCGCCCAACACCATAG                                                       >  SRR4302537.4531563/1‑94 (MQ=60)

                                         TGGAAGTCTTTGCTTTCGGCAGCCTGTGCATTATGTCGGAAGGTCGTTGCTACCTGTCGTCGTATCTGACGGGTGAGTCGCCCAACACCATAGG                                                      <  SRR4302537.6448547/94‑1 (MQ=60)

                                         TGGAAGTCTTTGCTTTCGGCAGCCTGTGCATTATGTCGGAAGGTCGTTGCTACCTGTCGTCGTATCTGACGGGTGAGTCGCCCAACACCATAGG                                                      <  SRR4302537.45257/94‑1 (MQ=60)

                                         TGGAAGTCTTTGCTTTCGGCAGCCTGTGCATTATGTCGGAAGGTCGTTGCTACCTGTCGTCGTATCTGACGGGTGAGTCGCCCAACACCATAGG                                                      >  SRR4302537.5256168/1‑94 (MQ=60)

                                         TGGAAGTCTTTGCTTTCGGCAGCCTGTGCATTATGTCGGAAGGTCGTTGCTACCTGTCGTCGTATCTGACGGGTGAGTCGCCCAACACCATAGG                                                      <  SRR4302537.4869439/94‑1 (MQ=60)

                                         TGGAAGTCTTTGCTTTCGGCAGCCTGTGCATTATGTCGGAAGGTCGTTGCTACCTGTCGTCGTATCTGACGGGTGAGTCGCCCAACACCATAGG                                                      <  SRR4302537.2380045/94‑1 (MQ=60)

                                         TGGAAGTCTTTGCTTTCGGCAGCCTGTGCATTATGTCGGAAGGTCGTTGCTACCTGTCGTCGTATCTGACGGGTGAGTCGCCCAACACCATAGG                                                      >  SRR4302537.871278/1‑94 (MQ=60)

                                          GGAAGTCTTTGCTTTCGGCAGCCTGTGCATTATGTCGGAAGGTCGTTGCTACCTGTCGTCGTATCTGACGGGTGAGTCGCCCAACACCATAGGC                                                     >  SRR4302537.3402178/1‑94 (MQ=60)

                                          GGAAGTCTTTGCTTTCGGCAGCCTGTGCATTATGTCGGAAGGTCGTTGCTACCTGTCGTCGTATCTGACGGGTGAGTCGCCCAACACCATAGGC                                                     <  SRR4302537.3677668/94‑1 (MQ=60)

                                          GGAAGTCTTTGCTTTCGGCAGCCTGTGCATTATGTCGGAAGGTCGTTGCTACCTGTCGTCGTATCTGACGGGTGAGTCGCCCAACACCATAGGC                                                     <  SRR4302537.5264337/94‑1 (MQ=60)

                                           GAAGTCTTTGCTTTCGGCAGCCTGTGCATTATGTCGGAAGGTCGTTGCTACCTGTCGTCGTATCTGACGGGTGAGTCGCCCAACACCATAGGCG                                                    >  SRR4302537.1588053/1‑94 (MQ=60)

                                            AAGTCTTTGCTTTCGGCAGCCTGTGCATTATGTCGGAAGGTCGTTGCTACCTGTCGTCGTATCTGACGGGTGAGTCGCCCAACACCATAGGCGC                                                   >  SRR4302537.5356399/1‑94 (MQ=60)

                                             AGTCTTTGCTTTCGGCAGCCTGTGCATTATGTCGGAAGGTCGTTGCTACCTGTCGTCGTATCTGACGGGTGAGTCGCCCAACACCATAGGCGCG                                                  <  SRR4302537.3694537/94‑1 (MQ=60)

                                             AGTCTTTGCTTTCGGCAGCCTGTGCATTATGTCGGAAGGTCGTTGCTACCTGTCGTCGTATCTGACGGGCGAGTCGCCCAACACCATAGGCGCG                                                  >  SRR4302537.6218635/1‑94 (MQ=60)

                                              GTCTTTGCTTTCGGCAGCCTGTGCATTATGTCGGAAGGTCGTTGCTACCTGTCGTCGTATCTGACGGGTGAGTCGCCCAACACCATAGGCGCGT                                                 <  SRR4302537.2737610/94‑1 (MQ=60)

                                               TCTTTGCTTTCGGCAGCCTGTGCATTATGTCGGAAGGTCGTTGCTATCTGTCGTCGTATCTGACGGGTGAGTCGCCCAACACCATAGGCGCGTG                                                <  SRR4302537.595664/94‑1 (MQ=60)

                                               TCTTTGCTTTCGGCAGCCTGTGCATTATGTCGGAAGGTCGTTGCTACCTGTCGTCGTATCTGACGGGTGAGTCGCCCAACACCATAGGCGCGTG                                                <  SRR4302537.4074590/94‑1 (MQ=60)

                                               TCTTTGCTTTCGGCAGCCTGTGCATTATGTCGGAAGGTCGTTGCTACCTGTCGTCGTATCTGACGGGTGAGTCGCCCAACACCATAGGCGCGTG                                                <  SRR4302537.3403303/94‑1 (MQ=60)

                                                CTTTGCTTTCGGCAGCCTGTGCATTATGTCGGAAGGTCGTTGCTACCTGTCGTCGTATCTGACGGGTGAGTCGCCCAACACCATAGGCGCGTGT                                               >  SRR4302537.1706119/1‑94 (MQ=60)

                                                CTTTGCTTTAGGCAGCCTGTGCATTATGTCGGAAGGTCGTTGCTACCTGTCGTCGTATCTGACGGGTGAGTCGCCCAACACCATAGGCGCGTGT                                               <  SRR4302537.3290426/94‑1 (MQ=60)

                                                 TTTGCTTTCGGCAGCCTGTGCATTATGTCGGAAGGTCGTTGCTACCTGTCGTCGTATCTGACGGGTGAGTCGCCCAACACCATAGGCGCGTGTT                                              <  SRR4302537.1154529/94‑1 (MQ=60)

                                                  TTGCTTTCGGCAGCCTGTGCATTATGTCGGAAGGTCGTTGCTACCTGTCGTCGTATCTGACGGGTGAGTCGCCCAACACCATAGGCGCGTGTTC                                             <  SRR4302537.5707218/94‑1 (MQ=60)

                                                  TTGCTTTCGGCAGCCTGTGCATTATGTCGGAAGGTCGTTGCTACCTGTCGTCGTATCTGACGGGTGAGTCGCCCAACACCATAGGCGCGTGTTC                                             >  SRR4302537.6118452/1‑94 (MQ=60)

                                                  TTGCTTTCGGCAGCCTGTGCATTATGTCGGAAGGTCGTTGCTACCTGTCGTCGTATCTGACGGGTGAGTCGCCCAACACCATAGGCGCGTGTTC                                             <  SRR4302537.5017569/94‑1 (MQ=60)

                                                   TGCTTTCGGCAGCCTGTGCATTATGTCGGAAGGTCGTTGCTACCTGTCGTCGTATCTGACGGGTGAGTCGCCCAACACCATAGGCGCGTGTTCT                                            >  SRR4302537.6458805/1‑94 (MQ=60)

                                                   TGCTTTCGGCAGCCTGTGCATTATGTCGGAAGGTCGTTGCTACCTGTCGTCGTATCTGACGGGTGAGTCGCCCAACACCATAGGCGCGTGTTCT                                            >  SRR4302537.2605288/1‑94 (MQ=60)

                                                   TGCTTTCGGCAGCCTGTGCATTATGTCGGAAGGTCGTTGCTACCTGTCGTCGTATCTGACGGGTGAGTCGCCCAACACCATAGGCGCGTGTTCT                                            >  SRR4302537.5393834/1‑94 (MQ=60)

                                                     CTTTCGGCAGCTTGTGCATTATGTCGGAAGGTCGTTGCTACCTGTCGTCGTATCTGACGGGTGAGTCGCCCAACACCATAGGCGCGTGTTCTCC                                          <  SRR4302537.1895650/94‑1 (MQ=60)

                                                     CGTTCGGCAGCCTGTGCATTATGTCGGAAGGTCGTTGCTACCTGTCGTCGTATCTGACGGGTGAGTCGCCCAACACCATAGGCGCGTGTTCTCC                                          >  SRR4302537.6136891/1‑94 (MQ=60)

                                                      TTTCGGCAGCCTGTGCATTATGTCGGAAGGTCGTTGCTACCTGTCGTCGTATCTGACGGGTGAGTCGCCCAACACCATAGGCGCGTGTTCTCCG                                         <  SRR4302537.1272694/94‑1 (MQ=60)

                                                      TTTCGGCAGCCTGTGCATTATGTCGGAAGGTCGTTGCTACCTGTCGTCGTATCTGACGGGTGAGTCGCCCAACACCATAGGCGCGTGTTCTCCG                                         <  SRR4302537.6302586/94‑1 (MQ=60)

                                                       TTCGGCAGCCTGTGCATTATGTCGGAAGGTCGTTGCTACCTGTCGTCGTATCTGACGGGTGAGTCGCCCAACACCATAGGCGCGTGTTCTCCGG                                        >  SRR4302537.2416765/1‑94 (MQ=60)

                                                        TCGGCAGCCTGTGCATTATGTCGGAAGGTCGTTGCTACCTGTCGTCGTATCTGACGGGTGAGTCGCCCAACACCATAGGCGCGTGTTCTCCGGC                                       <  SRR4302537.3997875/94‑1 (MQ=60)

                                                        TCGGCAGCCTGTGCATTATGTCGGAAGGTCGTTGCTACCTGTCGTCGTATCTGACGGGTGAGTCGCCCAACACCATAGGCGCGTGTTCTCCGGC                                       >  SRR4302537.2831572/1‑94 (MQ=60)

                                                         ctgcagcatgttcagTATGTCGGAAGGTCGTTGCTACCGGGCGGCGTATCTGGCGGTTGAGTCGCCCAACACCATAGGCGCGGGTTCTCCGGCC                                      <  SRR4302537.1299342/79‑1 (MQ=49)

                                                         CGTCAGCCTGTGCATTATGTCGGAAGGTCGTTGCTACCTGTCGTCGTATCTGACGGGTGAGTCGCCCAACACCATAGGCGCGTGTTCTCCGGCC                                      >  SRR4302537.4961259/1‑94 (MQ=60)

                                                            CAGCCTGTGCATTATGTCGGAAGGTCGTTGCTACCTGTCGTCGTATCTGACGGGTGAGTCGCCCAACACCATAGGCGCGTGTTCTCCGGCCCGT                                   >  SRR4302537.5505758/1‑94 (MQ=60)

                                                             AGCCTGTGCATTATGTCGGAAGGTCGTTGCTACCTGTCGTCGTATCTGACGGGTGAGTCGCCCAACACCATAGGCGCGTGTTCTCCGGCCCGTT                                  <  SRR4302537.5006901/94‑1 (MQ=60)

                                                              GCCTGTGCATTATGTCGGAAGGTCGTTGCTACCTGTCGTCGTATCTGACGGGTGAGTCGCCCAACACCATAGGCGCGTGTTCTCCGGCCCGTTT                                 >  SRR4302537.4525249/1‑94 (MQ=60)

                                                              GCCTGTGCATTATGTCGGAAGGTCGTTGCTACCTGTCGTCGTATCTGACGGGTGAGTCGCCCAACACCATAGGCGCGTGTTCTCCGGCCCGTTT                                 <  SRR4302537.6197557/94‑1 (MQ=60)

                                                                CTGTGCATTATGTCGGAAGGTCGTTGCTACCTGTCGTCGTATCTGACGGGTGAGTCGCCCAACAGCATAGGCGCGTGGTCTCCGGCCCGTTTCG                               >  SRR4302537.3376447/1‑94 (MQ=60)

                                                                CTGTGCATTATGTCGGAAGGTCGTTGCTACCTGTCGTCGTATCTGACGGGTGAGTCGCCCAACACCATAGGCGCGTGTTCTCCGGCCCGTTTCT                               >  SRR4302537.3691762/1‑94 (MQ=60)

                                                                CTGTGCATTATGTCGGAAGGTCGTTGCTACCTGTCGTCGTATCTGACGGGTGAGTCGCCCAACACCATAGGCGCGTGTTCTCCGGCCCGTTTCG                               >  SRR4302537.3417984/1‑94 (MQ=60)

                                                                CTGTGCATTATGTCGGAAGGTCGTTGCTACCTGTCGTCGTATCTGACGGGTGAGTCGCCCAACACCATAGGCGCGTGTTCTCCGGCCCGTTTCG                               >  SRR4302537.4940619/1‑94 (MQ=60)

                                                                CTGTGCATTATGTCGGAAGGTCGTTGCTACCTGTCGTCGTATCTGACGGGTGAGTCGCCCAACACCATAGGCGCGTGTTCTCCGGCCCGTTTCG                               >  SRR4302537.4654532/1‑94 (MQ=60)

                                                                CTGTGCATTATGTCGGAAGGTCGTTGCTACCTGTCGTCGTATCTGACGGGTGAGTCGCCCAACACCATAGGCGCGTGTTCTCCGCCCCGTTTCT                               >  SRR4302537.3514124/1‑94 (MQ=60)

                                                                 TGTGCATTATGTCGGAAGGTCGTTGCTACCTGTCGTCGTATCTGACGGGTGAGTCGCCCAACACCATAGACGCGTGTTCTCCGGCCCGTTTCGT                              >  SRR4302537.5541610/1‑94 (MQ=60)

                                                                   TGCATTATGTCGGAAGGTCGTTGCTACCTGTCGTCGTATCTGACGGGTGAGTCGCCCAACACCATAGGCGCGTGTTCTCCGGCCCGTTTCGTGC                            <  SRR4302537.2580193/94‑1 (MQ=60)

                                                                    GCATTATGTCGGAAGGTCGTTGCTACCTGTCGTCGTATCTGACGGGTGAGTCGCCCAACACCATAGGCGCGTGTTCTCCGGCCCGTTTCGTGCG                           <  SRR4302537.5129485/94‑1 (MQ=60)

                                                                     CATTATGTCGGAAGGTCGTTGCTACCTGTCGTCGTATCTGACGGGTGAGTCGCCCAACACCATAGGCGCGTGTTCTCCGGCCCGTTTCGTGCGC                          >  SRR4302537.5552932/1‑94 (MQ=60)

                                                                      ATTATGTCGGAAGGTCGTTGCTACCTGTCGTCGTATCTGACGGGTGAGTCGCCCAACACCATAGGCGCGTGTTCTCCGGCCCGTTTCGTGCGCT                         >  SRR4302537.1346295/1‑94 (MQ=60)

                                                                      ATTATGTCGGAAGGTCGTTGCTACCTGTCGTCGTATCTGACGGGTGAGTCGCCCAACACCATAGGCGCGTGTTCTCCGGCCCGTTTCGTGCGCT                         <  SRR4302537.266620/94‑1 (MQ=60)

                                                                      ATTATGTCGGAAGGTCGTTGCTACCTGTCGTCGTATCTGACGGGTGAGTCGCCCAACACCATAGGCGCGTGTTCTCCGGCCCGTTTCGTGCGCT                         >  SRR4302537.3736965/1‑94 (MQ=60)

                                                                      ATTATGTCGGAAGGTCGTTGCTACCTGTCGTCGTATCTGACGGGTGAGTCGCCCAACACCATAGGCGCGTGTTCTCCGGCCCGTTTCGTGCGCT                         >  SRR4302537.1112642/1‑94 (MQ=60)

                                                                      ATTATGTCGGAAGGTCGTTGCTACCTGTCGTCGTATCTGACGGGTGAGTCGCCCAACACCATAGGCGCGTGTTCTCCGGCCCGTTTCGTGCGCT                         <  SRR4302537.4383013/94‑1 (MQ=60)

                                                                       TTATGTCGGAAGGTCGTTGCTACCTGTCGTCGTATCTGACGGGTGAGTCGCCCAACACCATAGGCGCGTGTTCTCCGGCCCGTTTCGTGCGCTG                        >  SRR4302537.4913858/1‑94 (MQ=60)

                                                                       TTATGTCGGAAGGTCGTTGCTACCTGTCGTCGTATCTGACGGGTGAGTCGCCCAACACCATAGGCGCGTGTTCTCCGGCCCGTTTCGTGCGCTG                        >  SRR4302537.1324061/1‑94 (MQ=60)

                                                                         ATGTCGGAAGGTCGTTGCTACCTGTCGTCGTATCTGACGGGTGAGTCGCCCAACACCATAGGCGCGTGTTCTCCGGCCCGTTTCGTGCGCTGGC                      >  SRR4302537.4532887/1‑94 (MQ=60)

                                                                          TGTCGGAAGGTCGTTGCTACCTGTCGTCGTATCTGACGGGTGAGTCGCCCAACACCATAGGCGCGTGTTCTCCGGCCCGTTTCGTGCGCTGGCA                     >  SRR4302537.574941/1‑94 (MQ=60)

                                                                          TGTCGGAAGGTCGTTGCTACCTGTCGTCGTATCTGACGGGTGAGTCGCCCAACACCATAGGCGCGTGTTCTCCGGCCCGTTTCGTGCGCTGGCA                     <  SRR4302537.5264969/94‑1 (MQ=60)

                                                                             CGGAAGGTCGTTGCTACCTGTCGTCGTATCTGACGGGTGAGTCGCCCAACACCATAGGCGCGTGTTCTCCGGCCCGTTTCGTGCGCTGGCAACA                  >  SRR4302537.3305514/1‑94 (MQ=60)

                                                                             CGGAAGGTCGTTGCTACCTGTCGTCGTATCTGACGGGTGAGTCGCCCAACACCATAGGCGCGTGTTCTCCGGCCCGTTTCGTGCGCTGGCAACA                  >  SRR4302537.2430662/1‑94 (MQ=60)

                                                                              GGAAGGTCGTTGCTACCTGTCGTCGTATCTGACGGGTGAGTCGCCCAACACCATAGGCGCGTGTTCTCCGGCCCGTTTCGTGCGCTGGCAACAA                 >  SRR4302537.3593497/1‑94 (MQ=60)

                                                                              GGAAGGTCGTTGCTACCTGTCGTCGTATCTGACGGGTGAGTCGCCCAACACCATAGGCGCGTGTTCTCCGGCCCGTTTCGTGCGCTGGCAACAA                 >  SRR4302537.3742752/1‑94 (MQ=60)

                                                                              GGAAGGTCGTTGCTACCTGTCGTCGTATCTGACGGGTGAGTCGCCCAACACCATAGGCGCGGGTTATCCGGCCCGTTTCGTGCGCTGGCAACAA                 >  SRR4302537.2182243/1‑94 (MQ=60)

                                                                                AAGGTCGTTGCTATCTGTCGTCGTATCTGACGGGTGAGTCGCCCAACACCATAGGCGCGTGTTCTCCGGCCCGTTTCGTGCGCTGGCAACAAAC               <  SRR4302537.3827950/94‑1 (MQ=60)

                                                                                 AGGTTGTTGCTACCTGTCGTCGTATCTGACGGGTGAGTCGCCCAACACCATAGGCGCGTGTTCTCCGGCCCGGTTCGTGCGCTGGCAACAAACG              >  SRR4302537.2252632/1‑94 (MQ=60)

                                                                                   GTCGTTGCTACCTGTCGTCGTATCTGACGGGTGAGTCGCCCAACACCATAGGCGCGTGTTCTCCGGCCCGTTTCGTGCGCTGGCAACAAACGCC            <  SRR4302537.112356/94‑1 (MQ=60)

                                                                                   GTCGTTGCTACCTGTCGTCGTATCTGACGGGTGAGTCGCCCAACACCATAGGCGCGTGTTCTCCGGCCCGTTTCGTGCGCTGGCAACAAACGCC            <  SRR4302537.3988611/94‑1 (MQ=60)

                                                                                   GTCGTTGCTACCTGTCGTCGTATCTGACGGGTGAGTCGCCCAACACCATAGGCGCGTGTTCTCCGGCCCGTTTCGTGCGCTGGCAACAAACGCC            >  SRR4302537.796872/1‑94 (MQ=60)

                                                                                    TCGTTGCTACCTGTCGTCGTATCTGACGGGTGAGTCGCCCAACACCATAGGCGCGTGTTCTCCGGCCCGTTTCGTGCGCTGGCAACAAACGCCG           <  SRR4302537.4164672/94‑1 (MQ=60)

                                                                                     CGTTGCTACCTGTCGTCGTATCTGACGGGTGAGTCGCCCAACACCATAGGCGCGTGTTCTCCGGCCCGTTTCGTGCGCTGGCAAAAAACGCCGC          >  SRR4302537.2022356/1‑94 (MQ=60)

                                                                                      GTTGCTACCTGTCGTCGTATCTGACGGGTGAGTCGCCCAACACCATAGGCGCGTGTTCTCCGGCCCGTTTCGTGCGCTGGCAACAAACGCCGCA         <  SRR4302537.6496093/94‑1 (MQ=60)

                                                                                      GTTGCTACCTGTCGTCGTATCTGACGGGTGAGTCGCCCAACACCATAGGCGCGTGTTCTCCGGCCCGCTTCGTGCGCTGGCAACAAACGCCGCA         <  SRR4302537.439282/94‑1 (MQ=60)

                                                                                       TTGCTACCTGTCGTCGTATCTGACGGGTGAGTCGCCCAACACCATAGGCGCGTGTTCTCCGGCCCGTTTCGTGCGCTGGCAACAAACGCCGCAG        >  SRR4302537.4436799/1‑94 (MQ=60)

                                                                                        TGCTACCTGTCGTCGTATCTGACGGGTGAGTCGCCCAACACCATAGGCGCGTGTTCTCCGGCCCGTTTCGTGCGCTGGCAACAAACGCCGCACG       >  SRR4302537.972299/1‑94 (MQ=60)

                                                                                         GCTACCTGTCGTCGTATCTGACGGGTGAGTCGCCCAACACCATAGGCGTGTGTTCTGCGGTCCGTTTCGTGCGCTGGCAACAAAAGCCGCACGG      >  SRR4302537.5907157/1‑94 (MQ=60)

                                                                                         GCTACCTGTCGTCGTATCTGACGGGTGAGTCGCCCAACACCATAGGCGCGCGTTCTCCGGCCCGTTTCGTGCGCTGGCAACAAACGCCGCAGGG      >  SRR4302537.237549/1‑94 (MQ=60)

                                                                                          CTACCTGTCGTCGTATCTGACGGGTGAGTCGCCCAACACCATAGGCGCGTGTTCTCCGGCCCGTTTCGTGCGCTGGCAACAAACGCCGCAGGGG     <  SRR4302537.5191419/94‑1 (MQ=60)

                                                                                          CTACCTGTCGTCGTATCTGACGGGTGAGTCGCCCAACACCATAGGCGCGTGTTCTCCGGCCCGTTTCGTGCGCTGGCAACAAACGCCGCAGGGG     >  SRR4302537.873029/1‑94 (MQ=60)

                                                                                          CTACCTGTCGTCGTATCTGACGGGTGAGTCGCCCAACACCATAGGCGCGTGTTCTCCGGCCCGTTTCGTGCGCTGGCAACAAACGCCGCAGGGG     <  SRR4302537.5768845/94‑1 (MQ=60)

                                                                                            ACCTGTCGTCGTATCTGACGGGTGAGTCGCCCAACACCATAGGCGCGTGTTCTCCGGCCCGTTTCGTGCGCTGGCAACAAACGCCGCAGGGGCT   <  SRR4302537.743012/94‑1 (MQ=60)

                                                                                            ACCTGTCGTCGTATCTGACGGGTGAGTCGCCCAACACCATAGGCGCGTGTTCTCCGGCCCGTTTCGTGCGCTGGCAACAAACGCCGCAGGGGCT   <  SRR4302537.3751051/94‑1 (MQ=60)

                                                                                             CCTGTCGTCGTATCTGACGGGTGAGTCGCCCAACACCATAGGCGCGTGTTCTCCGGCCCGTTTCGTGCGCTGGCAACAAACGCCGCAGGGGCTG  <  SRR4302537.2814650/94‑1 (MQ=60)

TCATCAGGTGAAACAACTGGCACGGGTCACACCTGTACCACTGGAAGTCTTTGCTTTCGGCAGCCTGTGCATTATGTCGGAAGGTCGTTGCTATCTGTCGTCGTATCTGACGGGTGAGTCGCCCAACACCATAGGCGCGTGTTCTCCGGCCCGTTTCGTGCGCTGGCAACAAACGCCGCAGGGGCTG  >  NC_000913/3299563‑3299749

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 0 ≤` `ATCG/ATCG` `< 2 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`