Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I2 R1 882 254.4 12957588 98.0% 12698436 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,964,819 T→A L263Q (CTG→CAG)  rho → transcription termination factor Rho

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,964,8190TA99.6% 886.6 / NA 247L263Q (CTG→CAG) rhotranscription termination factor Rho
Reads supporting (aligned to +/- strand):  ref base T (0/0);  major base A (132/114);  minor base C (1/0);  total (133/114)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 251 total aligned reads displayed.

ACGAACCCGCATCTCGCCACGTTCAGGTTGCGGAAATGGTGATCGAGAAGGCCAAACGCCTGGTTGAGCACAAGAAAGACGTTATCATTCTGCTCGACTCCATCACTCGTCTGGCGCGCGCTTACAACACCGTTGTTCCGGCGTCAGGTAAAGTGTTGACCGGTGGTGTGGATGCCAACG  >  NC_000913/3964729‑3964908
                                                                                          |                                                                                         
aCGAGCACGCATCTCGCCACGTTCAGGTTGCGGAAATGGTGATCGAGAAGGCCAAACGCCTGGTTGAGCACAAGAAAGACGTTATCATTCAGc                                                                                         <  1:3322399/93‑1 (MQ=255)
  gAACCCGCATCTCGCCACGTTCAGGTTGCGGAAATGGTGATCGAGAAGGCCAAACGCCTGGTTGAGCACAAGAAAGACGTTATCATTCAGCTc                                                                                       <  2:5313228/93‑1 (MQ=255)
  gAACCCGCATCTCGCCACGTTCAGGTTGCGGAAATGGTGATCGAGAAGGCCAAACGCCTGGTTGAGCACAAGAAAGACGTTATCATTCAGCTc                                                                                       >  1:236264/1‑93 (MQ=255)
  gAACCCGCATCTCGCCACGTTCAGGTTGCGGAAATGGTGATCGAGAAGGCCAAACGCCTGGTTGAGCACAAGAAAGACGTTATCATTCAGCTc                                                                                       <  1:9735/93‑1 (MQ=255)
   aaCCCGCATCTCGCCACGTTCAGGTTGCGGAAATGGTGATCGAGAAGGCCAAACGCCTGGTTGAGCACAAGAAAGACGTTATCATTCAGCTCg                                                                                      >  1:319632/1‑93 (MQ=255)
   aaCCCGCATCTCGCCACGTTCAGGTTGCGGAAATGGTGATCGAGAAGGCCAAACGCCTGGTTGAGCACAAGAAAGACGTTATCATTCAGCTCg                                                                                      >  1:6344488/1‑93 (MQ=255)
     cccGCATCTCGCCACGTTCAGGTTGCGGAAATGGTGATCGAGAAGGCCAAACGCCTGGTTGAGCACAAGAAAGACGTTATCATTCAGCTCGAc                                                                                    <  2:6403792/93‑1 (MQ=255)
      ccGCATCTCGCCACGTTCAGGTTGCGGAAATGGTGATCGAGAAGGCCAAACGCCTGGTTGAGCACAAGAAAGACGTTATCATTCAGCTCGACt                                                                                   <  1:2451331/93‑1 (MQ=255)
       cGCATCTCGCCACGTTCAGGTTGCGGAAATGGTGATCGAGAAGGCCAAACGCCTGGTTGAGCACAAGAAAGACGTTATCATTCAGCTCGACTc                                                                                  <  2:672808/93‑1 (MQ=255)
       cGCATCTCGCCACGTTCAGGTTGCGGAAATGGTGATCGAGAAGGCCAAACGCCTGGTTGAGCACAAGAAAGACGTTATCATTCAGCTCGACTc                                                                                  <  2:28781/93‑1 (MQ=255)
         cATCTCGCCACGTTCAGGTTGCGGAAATGGTGATCGAGAAGGCCAAACGCCTGGTTGAGCACAAGAAAGACGTTATCATTCAGCTCGACTCCa                                                                                <  1:1895902/93‑1 (MQ=255)
         cATCTCGCCACGTTCAGGTTGCGGAAATGGTGATCGAGAAGGCCAAACGCCTGGTTGAGCACAAGAAAGACGTTATCATTCAGCTCGACTCCa                                                                                <  1:5491887/93‑1 (MQ=255)
         cATCTCGCCACGTTCAGGTTGCGGAAATGGTGATCGAGAAGGCCAAACGCCTGGTTGAGCACAAGAAAGACGTTATCATTCAGCTCGACTCCa                                                                                >  2:3762845/1‑93 (MQ=255)
           tctcGCCACGTTCAGGTTGCGGAAATGGTGATCGAGAAGGCCAAACGCCTGGTTGAGCACAAGAAAGACGTTATCATTCAGCTCGACTCCATc                                                                              <  1:5877120/93‑1 (MQ=255)
           tctcGCCACGTTCAGGTTGCGGAAATGGTGATCGAGAAGGCCAAACGCCTGGTTGAGCACAAGAAAGACGTTATCATTCAGCTCGACTACATc                                                                              >  2:1474355/1‑93 (MQ=255)
             tcGCCACGTTCAGGTTGCGGAAATGGTGATCGAGAAGGCCAAACGCCTGGTTGAGCACAAGAAAGACGTTATCATTCAGCTCGACTCCATCAc                                                                            <  1:4162999/93‑1 (MQ=255)
             tcGCCACGTTCAGGTTGCGGAAATGGTGATCGAGAAGGCCAAACGCCTGGTTGAGCACAAGAAAGACGTTATCATTCAGCTCGACTCCATCAc                                                                            <  2:871588/93‑1 (MQ=255)
               gCCACGTTCAGGTTGCGGAAATGGTGATCGAGAAGGCCAAACGCCTGGTTGAGCACAAGAAAGACGTTATCATTCAGCTCGACTCCATCACTc                                                                          <  1:2147097/93‑1 (MQ=255)
               gCCACGTTCAGGTTGCGGAAATGGTGATCGAGAAGGCCAAACGCCTGGTTGAGCACAAGAAAGACGTTATCATTCAGCTCGACTCCATCACTc                                                                          >  2:2822316/1‑93 (MQ=255)
                ccACGTTCAGGTTGCGGAAATGGTGATCGAGAAGGCCAAACGCCTGGTTGAGCACAAGAAAGACGTTATCATTCAGCTCGACTCCATCACTCg                                                                         <  1:2718669/93‑1 (MQ=255)
                ccACGTTCAGGTTGCGGAAATGGTGATCGAGAAGGCCAAACGCCTGGTTGAGCACAAGAAAGACGTTATCATTCAGCTCGACTCCATCACTCg                                                                         <  2:443534/93‑1 (MQ=255)
                ccACGTTCAGGTTGCGGAAATGGTGATCGAGAAGGCCAAACGCCTGGTTGAGCACAAGAAAGACGTTATCATTCAGCTCGACTCCATCACTCg                                                                         >  1:3599801/1‑93 (MQ=255)
                ccACGTTCAGGTTGCGGAAATGGTGATCGAGAAGGCCAAACGCCTGGTTGAGCACAAGAAAGACGTTATCATTCAGCTCGACTACATCACtag                                                                         >  2:3407072/1‑91 (MQ=255)
                  aCGTTCAGGTTGCGGAAATGGTGATCGAGAAGGCCAAACGCCTGGTTGAGCACAAGAAAGACGTTATCATTCAGCTCGACTCCATCACTCGTc                                                                       <  1:3432585/93‑1 (MQ=255)
                  aCGTTCAGGTTGCGGAAATGGTGATCGAGAAGGCCAAACGCCTGGTTGAGCACAAGAAAGACGTTATCATTCAGCTCGACTCCATCACTCGTc                                                                       <  1:1374765/93‑1 (MQ=255)
                   cGTTCAGGTTGCGGAAATGGTGATCGAGAAGGCCAAACGCCTGGTTGAGCACAAGAAAGACGTTATCATTCAGCTCGACTCCATCACTCGTCt                                                                      <  1:6115919/93‑1 (MQ=255)
                     ttCAGGTTGCGGAAATGGTGATCGAGAAGGCCAAACGCCTGGTTGAGCACAAGAAAGACGTTATCATTCAGCTCGACTCCATCACTCGTCTgg                                                                    >  2:546780/1‑93 (MQ=255)
                     ttCAGGTTGCGGAAATGGTGATCGAGAAGGCCAAACGCCTGGTTGAGCACAAGAAAGACGTTATCATTCAGCTCGACTCCATCACTCGTCTgg                                                                    >  2:1107342/1‑93 (MQ=255)
                     ttCAGGTTGCGGAAATGGTGATCGAGAAGGCCAAACGCCTGGTTGAGCACAAGAAAGACGTTATCATTCAGCTCGACTCCATCACTCGTCTgg                                                                    >  2:1202582/1‑93 (MQ=255)
                     ttCAGGTTGCGGAAATGGTGATCGAGAAGGCCAAACGCCTGGTTGAGCACAAGAAAGACGTTATCATTCAGCTCGACTCCATCACTCGTCTgg                                                                    >  2:1261841/1‑93 (MQ=255)
                     ttCAGGTTGCGGAAATGGTGATCGAGAAGGCCAAACGCCTGGTTGAGCACAAGAAAGACGTTATCATTCAGCTCGACTCCATCACTCGTCTgg                                                                    >  1:1475126/1‑93 (MQ=255)
                      tCAGGTTGCGGAAATGGTGATCGAGAAGGCCAAACGCCTGGTTGAGCACAAGAAAGACGTTATCATTCAGCTCGACTCCATCACTCGTCTGgc                                                                   >  2:2537717/1‑93 (MQ=255)
                      tCAGGTTGCGGAAATGGTGATCGAGAAGGCCAAACGCCTGGTTGAGCACAAGAAAGACGTTATCATTCAGCTCGACTCCATCACTCGTCTGgc                                                                   >  2:2794885/1‑93 (MQ=255)
                            tGCGGAAATGGTGATCGAGAAGGCCAAACGCCTGGTTGAGCACAAGAAAGACGTTATCATTCAGCTCGACTCCATCACTCGTCTGgcgcgcgc                                                             <  1:2254895/93‑1 (MQ=255)
                             gCGGAAATGGTGATCGAGAAGGCCAAACGCCTGGTTGAGCACAAGAAAGACGTTATCATTCAGCTCGACTCCATCACTCGTCTGGCGCGCGCt                                                            <  1:1316135/93‑1 (MQ=255)
                               ggAAATGGTGATCGAGAAGGCCAAACGCCTGGTTGAGCACAAGAAAGACGTTATCATTCAGCTCGACTCCATCACTCGTCTGGCGCGCGCTTa                                                          >  1:4689338/1‑93 (MQ=255)
                                gAAATGGTGATCGAGAAGGCCAAACGCCTGGTTGAGCACAAGAAAGACGTTATCATTCAGCTCGACTCCATCACTCGTCTGGCGCGCGCTTac                                                         >  2:5653674/1‑93 (MQ=255)
                                gAAATGGTGATCGAGAAGGCCAAACGCCTGGTTGAGCACAAGAAAGACGTTATCATTCAGCTCGACTCCATCACTCGTCTGGCGCGCGCTTac                                                         <  1:4813322/93‑1 (MQ=255)
                                  aaTGGTGATCGAGAAGGCCAAACGCCTGGTTGAGCACAAGAAAGACGTTATCATTCAGCTCGACTCCATCACTCGTCTGGCGCGCGCTTacaa                                                       <  1:4780919/93‑1 (MQ=255)
                                    tGGTGATCGAGAAGGCCAAACGCCTGGTTGAGCACAAGAAAGACGTTATCATTCAGCTCGACTCCATCACTCGTCTGGCGCGCGCTTacaaca                                                     <  1:4401405/93‑1 (MQ=255)
                                      gTGATCGAGAAGGCCAAACGCCTGGTTGAGCACAAGAAAGACGTTATCATTCAGCTCGACTCCATCACTCGTCTGGCGCGCGCTTACAACAcc                                                   >  2:4852468/1‑93 (MQ=255)
                                       tGATCGAGAAGGCCAAACGCCTGGTTGAGCACAAGAAAGACGTTATCATTCAGCTCGACTCCATCACTCGTCTGGCGCGCGCTTACAACACCg                                                  <  2:5046362/93‑1 (MQ=255)
                                        gATGGAGAAGGCCAAACGCCTGGTTGAGCACAAGAAAGACGTTATCATTCAGCTCGACTCCATCACTCGTCTGGCGCGCGCTTACAACACCgt                                                 <  2:2351502/93‑1 (MQ=255)
                                        gATCGAGAAGGCCAAACGCCTGGTTGAGCACAAGAAAGACGTTATCATTCAGCTCGACTCCATCACTCGTCTGGCGCGCGCTTACAACACCgt                                                 >  1:4040027/1‑93 (MQ=255)
                                          tCGAGAAGGCCAAACGCCTGGTTGAGCACAAGAAAGACGTTATCATTCAGCTCGACTCCATCACTCGTCTGGCGCGCGCTTACAACACCgttg                                               >  1:456930/1‑93 (MQ=255)
                                             agaAGGCCAAACGCCTGGTTGAGCACAAGAAAGACGTTATCATTCAGCTCGACTCCATCACTCGTCTGGCGCGCGCTTACAACACCGTTGTTc                                            <  1:3069598/93‑1 (MQ=255)
                                             agaAGGCCAAACGCCTGGTTGAGCACAAGAAAGACGTTATCATTCAGCTCGACTCCATCACTCGTCTGGCGCGCGCTTACAACACCGTTGTTc                                            >  2:4909044/1‑93 (MQ=255)
                                              gaAGGCCAAACGCCTGGTTGAGCACAAGAAAGACGTTATCATTCAGCTCGACTCCATCACTCGTCTGGCGCGCGCTTACAACACCGTTGTTcc                                           <  2:6019118/93‑1 (MQ=255)
                                                 ggCCAAACGCCTGGTTGAGCACAAGAAAGACGTTATCATTCAGCTCGACTCCATCACTCGTCTGGCGCGCGCTTACAACACCGTTGTTCCGGc                                        <  1:3707043/93‑1 (MQ=255)
                                                  gCCAAACGCCTGGTTGAGCACAAGAAAGACGTTATCATTCAGCTCGACTCCATCACTCGTCTGGCGCGCGCTTACAACACCGTTGTTCCGGCg                                       >  1:4338289/1‑93 (MQ=255)
                                                   ccAAACGCCTGGTTGAGCACAAGAAAGACGTTATCATTCAGCTCGACTCCATCACTCGTCTGGCGCGCGCTTACAACACCGTTGTTCCGGCGt                                      >  1:1614643/1‑93 (MQ=255)
                                                    cAAACGCCTGGTTGAGCACAAGAAAGACGTTATCATTCAGCTCGACTCCATCACTCGTCTGGCGCGCGCTTACAACACCGTTGTTCCGGCGTc                                     <  1:2869196/93‑1 (MQ=255)
                                                     aaaCGCCTGGTTGAGCACAAGAAAGACGTTATCATTCAGCTCGACTCCATCACTCGTCTGGCGCGCGCTTACAACACCGTTGTTCCGGCGTCa                                    >  2:1467307/1‑93 (MQ=255)
                                                     aaaCGCCTGGTTGAGCACAAGAAAGACGTTATCATTCAGCTCGACTCCATCACTCGTCTGGCGCGCGCTTACAACACCGTTGTTCCGGCGTCa                                    >  2:5090250/1‑93 (MQ=255)
                                                      aaCGCCTGGTTGAGCACAAGAAAGACGTTATCATTCAGCTCGACTCCATCACTCGTCTGGCGCGCGCTTACAACACCGTTGTTCCGGCGTCAg                                   <  2:500558/93‑1 (MQ=255)
                                                      aaCGCCTGGTTGAGCACAAGAAAGACGTTATCATTCAGCTCGACTCCATCACTCGTCTGGCGCGCGCTTACAACACCGTTGTTCCGGCGTCAg                                   >  1:4477724/1‑93 (MQ=255)
                                                      aaCGCCTGGTTGAGCACAAGAAAGACGTTATCATTCAGCTCGACTCCATCACTCGTCTGGCGCGCGCTTACAACACCGTTGTTCCGGCGTCAg                                   <  2:5849671/93‑1 (MQ=255)
                                                      aaCGCCTGGTTGAGCACAAGAAAGACGTTATCATTCAGCTCGACTCCATCACTCGTCTGGCGCGCGCTTACAACACCGTTGTTCCGGCGTCAg                                   >  2:1857983/1‑93 (MQ=255)
                                                      aaCGCCTGGTTGAGCACAAGAAAGACGTTATCATTCAGCTCGACTCCATCACTCGTCTGGCGCGCGCTTACAACACCGTTGTTCCGGCGTCAg                                   >  2:2199009/1‑93 (MQ=255)
                                                        cGCCTGGTTGAGCACAAGAAAGACGTTATCATTCAGCTCGACTCCATCACTCGTCTGGCGCGCGCTTACAACACCGTTGTTCCGGCGTCAGGt                                 >  2:421801/1‑93 (MQ=255)
                                                         gCCTGGTTGAGCACAAGAAAGACGTTATCATTCAGCTCGACTCCATCACTCGTCTGGCGCGCGCTTACAACACCGTTGTTCCGGCGTCAGGTa                                >  1:1710722/1‑93 (MQ=255)
                                                         gCCTGGTTGAGCACAAGAAAGACGTTATCATTCAGCTCGACTCCATCACTCGTCTGGCGCGCGCTTACAACACCGTTGTTCCGGCGTCAGGTa                                >  1:570957/1‑93 (MQ=255)
                                                           cTGGTTGAGCACAAGAAAGACGTTATCATTCAGCTCGACTCCATCACTCGTCTGGCGCGCGCTTACAACACCGTTGTTCCGGCGTCAGGTaaa                              >  2:42425/1‑93 (MQ=255)
                                                           cTGGTTGAGCACAAGAAAGACGTTATCATTCAGCTCGACTCCATCACTCGTCTGGCGCGCGCTTACAACACCGTTGTTCCGGCGTCAGGTaaa                              >  2:5561892/1‑93 (MQ=255)
                                                           cTGGTTGAGCACAAGAAAGACGTTATCATTCAGCTCGACTCCATCACTCGTCTGGCGCGCGCTTACAACACCGTTGTTCCGGCGTCAGGTaaa                              <  2:3935042/93‑1 (MQ=255)
                                                              gTTGAGCACAAGAAAGACGTTATCATTCAGCTCGACTCCATCACTCGTCTGGCGCGCGCTTACAACACCGTTGTTCCGGCGTCAGGTAAAgtg                           >  2:1032618/1‑93 (MQ=255)
                                                               ttGAGCACAAGAAAGACGTTATCATTCAGCTCGACTCCATCACTCGTCTGGCGCGCGCTTACAACACCGTTGTTCCGGCGTCAGGTAAAgtgt                          <  1:2605033/93‑1 (MQ=255)
                                                               ttGAGCACAAGAAAGACGTTATCATTCAGCTCGACTCCATCACTCGTCTGGCGCGCGCTTACAACACCGTTGTTCCGGCGTCAGGTAAAgtgt                          <  1:1432385/93‑1 (MQ=255)
                                                               ttGAGCACAAGAAAGACGTTATCATTCAGCTCGACTCCATCACTCGTCTGGCGCGCGCTTACAACACCGTTGTTCCGGCGTCAGGTAAAgtgt                          <  2:4349137/93‑1 (MQ=255)
                                                                tGAGCACAAGAAAGACGTTATCATTCAGCTCGACTCCATCACTCGTCTGGCGCGCGCTTACAACACCGTTGTTCCGGCGTCAGGTAAAGTGtt                         <  2:3655444/93‑1 (MQ=255)
                                                                   gCACAAGAAAGACGTTATCATTCAGCTCGACTCCATCACTCGACTGGCGCGCGCTTACAACACCGTTGTTCCGGCGTCAGGTAAAGTGTTGAc                      >  1:3471733/1‑93 (MQ=255)
                                                                    cacaAGAAAGACGTTATCATTCAGCTCGACTCCATCACTCGTCTGGCGCGCGCTTACAACACCGTTGTTCCGGCGTCAGGTAAAGTGTTGAcc                     >  1:2339750/1‑93 (MQ=255)
                                                                     acaAGAAAGACGTTATCATTCAGCTCGACTCCATCACTCGTCTGGCGCGCGCTTACAACACCGTTGTTCCGGCGTCAGGTAAAGTGTTGACCg                    >  1:5389936/1‑93 (MQ=255)
                                                                      caAGAAAGACGTTATCATTCAGCTCGACTCCATCACTCGTCTGGCGCGCGCTTACAACACCGTTGTTCCGGCGTCAGGTAAAGTGTTGACCgg                   <  2:2432873/93‑1 (MQ=255)
                                                                      caAGAAAGACGTTATCATTCAGCTCGACTCCATCACTCGTCTGGCGCGCGCTTACAACACCGTTGTTCCGGCGTCAGGTAAAGTGTTGACCgg                   >  2:3456675/1‑93 (MQ=255)
                                                                      caAGAAAGACGTTATCATTCAGCTCGACTCCATCACTCGTCTGGCGCGCGCTTACAACACCGTTGTTCCGGCGTCAGGTAAAGTGTTGACCgg                   >  1:204407/1‑93 (MQ=255)
                                                                      caAGAAAGACGTTATCATTCAGCTCGACTCCATCACTCGTCTGGCGCGCGCTTACAACACCGTTGTTCCGGCGTCAGGTAAAGTGTTGACCgg                   <  1:6171688/93‑1 (MQ=255)
                                                                       aagaaagaCGTTATCATTCAGCTCGACTCCATCACTCGTCTGGCGCGCGCTTACAACACCGTTGTTCCGGCGTCAGGTAAAGTGTTGACCggt                  <  2:4133586/93‑1 (MQ=255)
                                                                       aagaaagaCGTTATCATTCAGCTAGACTCCATCACTCGTCTGGCGCGCGCTTACAACACCGTTGTTCCGGCGTCAGGTAAAGTGTTGACCggt                  >  2:2957532/1‑93 (MQ=255)
                                                                        agaaagaCGTTATCATTCAGCTCGACTCCATCACTCGTCTGGCGCGCGCTTACAACACCGTTGTTCCGGCGTCAGGTAAAGTGTTGACCggtg                 <  2:1981412/93‑1 (MQ=255)
                                                                         gaaagaCGTTATCATTCAGCTCGACTCCATCACTCGTCTGGCGCGCGCTTACAACACCGTTGTTCCGGCGTCAGGTAAAGTGTTGACCggtgg                >  2:1868656/1‑93 (MQ=255)
                                                                         gaaagaCGTTATCATTCAGCTCGACTCCATCACTCGTCTGGCGCGCGCTTACAACACCGTTGTTCCGGCGTCAGGTAAAGTGTTGACCggtgg                >  2:5408575/1‑93 (MQ=255)
                                                                            agaCGTTATCATTCAGCTCGACTCCATCACTCGTCTGGCGCGCGCTTACAACACCGTTGTTCCGGCGTCAGGTAAAGTGTTGACCGGTGgtgt             <  1:2271116/93‑1 (MQ=255)
                                                                             gaCGTTATCATTCAGCTCGACTCCATCACTCGTCTGGCGCGCGCTTACAACACCGTTGTTCCGGCGTCAGGTAAAGTGTTGACCGGTGGtgtg            >  2:2779845/1‑93 (MQ=255)
                                                                             gaCGTTATCATTCAGCTCGACTCCATCACTCGTCTGGCGCGCGCTTACAACACCGTTGTTCCGGCGTCAGGTAAAGTGTTGACCGGTGGtgtg            <  2:2626443/93‑1 (MQ=255)
                                                                             gaCGTTATCATTCAGCTCGACTCCATCACTCGTCTGGCGCGCGCTTACAACACCGTTGTTCCGGCGTCAGGTAAAGTGTTGACCGGTGGtgtg            <  1:5153592/93‑1 (MQ=255)
                                                                               cGTTATCATTCAGCTCGACTCCATCACTCGTCTGGCGCGCGCTTACAACACCGTTGTTCCGGCGTCAGGTAAAGTGTTGACCGGTGGTGTGGa          <  1:1049146/93‑1 (MQ=255)
                                                                               cGTTATCATTCAGCTCGACTCCATCACTCGTCTGGCGCGCGCTTACAACACCGTTGTTCCGGCGTCAGGTAAAGTGTTGACCGGTGGTGTGGa          <  2:3082007/93‑1 (MQ=255)
                                                                                gTTATCATTCAGCTCGACTCCATCACTCGTCTGGCGCGCGCTTACAACACCGTTGTTCCGGCGTCAGGTAAAGTGTTGACCGGTGGTGTGGAt         >  1:2200331/1‑93 (MQ=255)
                                                                                gTTATCATTCAGCTCGACTCCATCACTCGTCTGGCGCGCGCTTACAACACCGTTGTTCCGGCGTCAGGTAAAGTGTTGACCGGTGGTGTGGAt         >  1:4974927/1‑93 (MQ=255)
                                                                                  tATCATTCAGCTCGACTCCATCACTCGTCTGGCGCGCGCTTACAACACCGTTGTTCCGGCGTCAGGTAAAGTGTTGACCGGTGGTGTGGATGc       <  2:1760349/93‑1 (MQ=255)
                                                                                  tATCATTCAGCTCGACTCCATCACTCGTCTGGCGCGCGCTTACAACACCGTTGTTCCGGCGTCAGGTAAAGTGTTGACCGGTGGTGTGGATGc       >  1:6451800/1‑93 (MQ=255)
                                                                                   aTCATTCAGCTCGACTCCATCACTCGTCTGGCGCGCGCTTACAACACCGTTGTTCCGGCGTCAGGTAAAGTGTTGACCGGTGGTGTGGATGcc      <  1:1232405/93‑1 (MQ=255)
                                                                                    tCATTCAGCTCGACTCCATCACTCGTCTGGCGCGCGCTTACAACACCGTTGTTCCGGCGTCAGGTAAAGTGTTGACCGGTGGTGTGGATGCCa     <  2:5948697/93‑1 (MQ=255)
                                                                                     cATTCAGCTCGACTCCATCACTCGTCTGGCGCGCGCTTACAACACCGTTGTTCCGGCGTCAGGTAAAGTGTTGACCGGTGGTGTGGATGCCaa    <  1:5211587/93‑1 (MQ=255)
                                                                                     cATTCAGCTCGACTCCATCACTCGTCTGGCGCGCGCTTACAACACCGTTGTTCCGGCGTCAGGTAAAGTGTTGACCGGTGGTGTGGATGACaa    <  2:6146912/93‑1 (MQ=255)
                                                                                      aTTCAGCTCGACTCCATCACTCGTCTGGCGCGCGCTTACAACACCGTTGTTCCGGCGTCAGGTAAAGTGTTGACCGGTGGTGTGGATGCCaag   >  1:715419/1‑92 (MQ=255)
                                                                                      aTTCAGCTCGACTCCATCACTCGTCTGGCGCGCGCTTACAACACCGTTGTTCCGGCGTCAGGTAAAGTGTTGACCGGTGGTGTGGATGCCAAc   >  1:4722088/1‑93 (MQ=255)
                                                                                       ttCAGCTCGACTCCATCACTCGTCTGGCGCGCGCTTACAACACCGTTGTTCCGGCGTCAGGTAAAGTGTTGACCGGTGGTGTGGATGGCAACg  >  1:2542910/1‑93 (MQ=255)
                                                                                       ttCAGCTCGACTCCATCACTCGTCTGGCGCGCGCTTACAACACCGTTGTTCCGGCGTCAGGTAAAGTGTTGACCGGTGGTGTGGATGCCAAcc  >  2:1678815/1‑92 (MQ=255)
                                                                                          |                                                                                         
ACGAACCCGCATCTCGCCACGTTCAGGTTGCGGAAATGGTGATCGAGAAGGCCAAACGCCTGGTTGAGCACAAGAAAGACGTTATCATTCTGCTCGACTCCATCACTCGTCTGGCGCGCGCTTACAACACCGTTGTTCCGGCGTCAGGTAAAGTGTTGACCGGTGGTGTGGATGCCAACG  >  NC_000913/3964729‑3964908

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
TTGACGAACCCGCATCTCGCCACGTTCAGGTTGCGGAAATGGTGATCGAGAAGGCCAAACGCCTGGTTGAGCACAAGAAAGACGTTATCATTCTGCTCGACTCCATCACTCGTCTGGCGCGCGCTTACAACACCGTTGTTCCGGCGTCAGGTAAAGTGTTGACCGGTGGTGTGGATGCCAACGCCCT  >  NC_000913/3964726‑3964912
                                                                                             |                                                                                             
TTGACGAACCCGCATCTCGCCACGTTCAGGTTGCGGAAATGGTGATCGAGAAGGCCAAACGCCTGGTTGAGCACAAGAAAGACGTTATCATTCA                                                                                               <  SRR4302537.2865781/94‑1 (MQ=60)
TTGACGAACCCGCATCTCGCCACGTTCAGGTTGCGGAAATGGTGATCGAGAAGGCCAAACGCCTGGTTGAGCACAAGAAAGACGTTATCATTCA                                                                                               <  SRR4302537.3025273/94‑1 (MQ=60)
 TGACGAACCCGCATCTCGCCACGTTCAGGTTGCGGAAATGGTGATCGAGAAGGCCAAACGCCTGGTTGAGCACAAGAAAGACGTTATCATTCAG                                                                                              <  SRR4302537.197570/94‑1 (MQ=60)
 TGACGAACCCGCATCTCGCCACGTTCAGGTTGCGGAAATGGTGATCGAGAAGGCCAAACGCCTGGTTGAGCACAAGAAAGACGTTATCATTCAG                                                                                              <  SRR4302537.658865/94‑1 (MQ=60)
 TGACGAACCCGCATCTCGCCACGTTCAGGTTGCGGAAATGGTGATCGAGAAGGCCAAACGCCTGGTTGAGCACAAGAAAGACGTTATCATTCAG                                                                                              <  SRR4302537.3751137/94‑1 (MQ=60)
   ACGAGCACGCATCTCGCCACGTTCAGGTTGCGGAAATGGTGATCGAGAAGGCCAAACGCCTGGTTGAGCACAAGAAAGACGTTATCATTCAGCT                                                                                            <  SRR4302537.3345901/94‑1 (MQ=60)
   ACGAACCCGCATCTCGCCACGTTCAGGTTGCGGAAATGGTGATCGAGAAGGCCAAACGCCTGGTTGAGCACAAGAAAGACGTTATCATTCAGCT                                                                                            <  SRR4302537.1614989/94‑1 (MQ=60)
   ACGAACCCGCATCTCGCCACGTTCAGGTTGCGGAAATGGTGATCGAGAAGGCCAAACGCCTGGTTGAGCACAAGAAAGACGTTATCATTCAGCT                                                                                            <  SRR4302537.5808428/94‑1 (MQ=60)
    CGAACCCGCATCTCGCCACGTTCAGGTTGCGGAAATGGTGATCGAGAAGGCCAAACGCCTGGTTGAGCACAAGAAAGACGTTATCATTCAGCTC                                                                                           >  SRR4302537.237930/1‑94 (MQ=60)
     GAACCCGCATCTCGCCACGTTCAGGTTGCGGAAATGGTGATCGAGAAGGCCAAACGCCTGGTTGAGCACAAGAAAGACGTTATCATTCAGCTCG                                                                                          <  SRR4302537.9804/94‑1 (MQ=60)
     GAACCCGCATCTCGCCACGTTCAGGTTGCGGAAATGGTGATCGAGAAGGCCAAACGCCTGGTTGAGCACAAGAAAGACGTTATCATTCAGCTCG                                                                                          >  SRR4302537.321847/1‑94 (MQ=60)
     GAACCCGCATCTCGCCACGTTCAGGTTGCGGAAATGGTGATCGAGAAGGCCAAACGCCTGGTTGAGCACAAGAAAGACGTTATCATTCAGCTCG                                                                                          >  SRR4302537.6387826/1‑94 (MQ=60)
       ACCCGCATCTCGCCACGTTCAGGTTGCGGAAATGGTGATCGAGAAGGCCAAACGCCTGGTTGAGCACAAGAAAGACGTTATCATTCAGCTCGAC                                                                                        >  SRR4302537.5528485/1‑94 (MQ=60)
        CCCGCATCTCGCCACGTTCAGGTTGCGGAAATGGTGATCGAGAAGGCCAAACGCCTGGTTGAGCACAAGAAAGACGTTATCATTCAGCTCGACT                                                                                       <  SRR4302537.4487591/94‑1 (MQ=60)
         CCGCATCTCGCCACGTTCAGGTTGCGGAAATGGTGATCGAGAAGGCCAAACGCCTGGTTGAGCACAAGAAAGACGTTATCATTCCGCTCGACTC                                                                                      >  SRR4302537.1283346/1‑94 (MQ=60)
         CCGCATCTCGCCACGTTCAGGTTGCGGAAATGGTGATCGAGAAGGCCAAACGCCTGGTTGAGCACAAGAAAGACGTTATCATTCAGCTCGACTC                                                                                      <  SRR4302537.2468575/94‑1 (MQ=60)
          CGCATCTCGCCACGTTCAGGTTGCGGAAATGGTGATCGAGAAGGCCAAACGCCTGGTTGAGCACAAGAAAGACGTTATCATTCAGCTCGACTCC                                                                                     >  SRR4302537.2287490/1‑94 (MQ=60)
          CGCATCTCGCCACGTTCAGGTTGCGGAAATGGTGATCGAGAAGGCCAAACGCCTGGTTGAGCACAAGAAAGACGTTATCATTCAGCTCGACTCC                                                                                     >  SRR4302537.1641431/1‑94 (MQ=60)
          CGCATCTCGCCACGTTCAGGTTGCGGAAATGGTGATCGAGAAGGCCAAACGCCTGGTTGAGCACAAGAAAGACGTGATCATTCAGCTCGACTCC                                                                                     >  SRR4302537.5699839/1‑94 (MQ=60)
            CATCTCGCCACGTTCAGGTTGCGGAAATGGTGATCGAGAAGGCCAAACGCCTGGTTGAGCACAAGAAAGACGTTATCATTCAGCTCGACTCCAT                                                                                   <  SRR4302537.1909075/94‑1 (MQ=60)
            CATCTCGCCACGTTCAGGTTGCGGAAATGGTGATCGAGAAGGCCAAACGCCTGGTTGAGCACAAGAAAGACGTTATCATTCAGCTCGACTCCAT                                                                                   <  SRR4302537.526833/94‑1 (MQ=60)
            CATCTCGCCACGTTCAGGTTGCGGAAATGGTGATCGAGAAGGCCAAACGCCTGGTTGAGCACAAGAAAGACGTTATCATTCAGCTCGACTCCAT                                                                                   <  SRR4302537.1463842/94‑1 (MQ=60)
            CATCTCGCCACGTTCAGGTTGCGGAAATGGTGATCGAGAAGGCCAAACGCCTGGTTGAGCACAAGAAAGACGTTATCATTCAGCTCGACTCCAT                                                                                   <  SRR4302537.5529377/94‑1 (MQ=60)
            CATCTCGCCACGTTCAGGTTGCGGAAATGGTGATCGAGAAGGCCAAACGCCTGGTTGAGCACAAGAAAGACGTTATCATTCAGCTCGACTCCAT                                                                                   <  SRR4302537.6172413/94‑1 (MQ=60)
            CATCTCGCCACGTTCAGGTTGCGGAAATGGTGATCGAGAAGGCCAAACGCCTGGTTGAGCACAAGAAAGACGTTATCATTCAGCTCGACTCCAT                                                                                   <  SRR4302537.741947/94‑1 (MQ=60)
             ATCTCGCCACGTTCAGGTTGCGGAAATGGTGATCGAGAAGGCCAAACGCCTGGTTGAGCACAAGAAAGACGTTATCATTCAGCTCGACTCCATC                                                                                  <  SRR4302537.1825110/94‑1 (MQ=60)
              TCTCGCCACGTTCAGGTTGCGGAAATGGTGATCGAGAAGGCCAAACGCCTGGTTGAGCACAAGAAAGACGTTATCATTCAGCTCGACTCCATCA                                                                                 <  SRR4302537.5917444/94‑1 (MQ=60)
               CTCGCCACGTTCAGGGTTCGGAAATGGTGATCGAGAAGGCCAAACGCCTGGTTGAGCACAAGAAAGACGTTATCATTCAGCTCGACTCCATCAC                                                                                <  SRR4302537.432269/94‑1 (MQ=60)
                TCGCCACGTTCAGGTTGCGGAAATGGTGATCGAGAAGGCCAAACGCCTGGTTGAGCACAAGAAAGACGTTATCATTCAGCTCGACTCCATCACT                                                                               <  SRR4302537.4192072/94‑1 (MQ=60)
                 CGCCACGTTCAGGTTGCGGAAATGGTGATCGAGAAGGCCAAACGCCTGGTTGAGCACAAGAAAGACGTTATCATTCAGCTCGACTCCATCACTC                                                                              <  SRR4302537.5246460/94‑1 (MQ=60)
                 CGCCACGTTCAGGTTGCGGAAATGGTGATCGAGAAGGCCAAACGCCTGGTTGAGCACAAGAAAGACGTTATCATTCAGCTCGACTCCATCACTC                                                                              >  SRR4302537.5497603/1‑94 (MQ=60)
                  GCCACGTTCAGGTTGCGGAAATGGTGATCGAGAAGGCCAAACGCCTGGTTGAGCACAAGAAAGACGTTATCATTCAGCTCGACTCCATCACTCT                                                                             >  SRR4302537.1237183/1‑94 (MQ=60)
                  GCCACGTTCAGGTTGCGGAAATGGTGATCGAGAAGGCCAAACGCCTGGTTGAGCACAAGAAAGACGTTATCATTCAGCTCGACTCCATCACTCG                                                                             >  SRR4302537.3625173/1‑94 (MQ=60)
                  GCCACGTTCAGGTTGCGGAAATGGTGATCGAGAAGGCCAAACGCCTGGTTGAGCACAAGAAAGACGTTATCATTCAGCTCGACTCCATCACTCG                                                                             <  SRR4302537.2162200/94‑1 (MQ=60)
                   CCACGTTCAGGTTGCGGAAATGGTGATCGAGAAGGCCAAACGCCTGGTTGAGCACAAGAAAGACGTTATCATTCAGCTCGACTCCATCACTCGT                                                                            <  SRR4302537.2737655/94‑1 (MQ=60)
                     ACGTTCAGGTTGCGGAAATGGTGATCGAGAAGGCCAAACGCCTGGTTGAGCACAAGAAAGACGTTATCATTCAGCTCGACTCCATCACTCGTCT                                                                          <  SRR4302537.1384363/94‑1 (MQ=60)
                     ACGTTCAGGTTGCGGAAATGGTGATCGAGAAGGCCAAACGCCTGGTTGAGCACAAGAAAGACGTTATCATTCAGCTCGACTCCATCACTCGTCT                                                                          <  SRR4302537.3456880/94‑1 (MQ=60)
                      CGTTCAGGTTGCGGAAATGGTGATCGAGAAGGCCAAACGCCTGGTTGAGCACAAGAAAGACGTTATCATTCAGCTCGACTCCATCACTCGTCTG                                                                         <  SRR4302537.6157945/94‑1 (MQ=60)
                      CGTTCAGGTTGCGGAAATGGTGATCGAGAAGGCCAAACGCCTGGTTGAGCACAAGAAAGACGTTATCATTCAGCTCGACTCCATCACTCGTCTG                                                                         <  SRR4302537.5799900/94‑1 (MQ=60)
                       GTTCAGGTTGCGGAAATGGTGATCGAGAAGGCCAAACGCCTGGTTGAGCACAAGAAAGACGTTATCATTCAGCTCGACTCCATCACTCGTCTGG                                                                        >  SRR4302537.1485377/1‑94 (MQ=60)
                       GTTCAGGTTGCGGAAATGGTGATCGAGAAGGCCAAACGCCTGGTTGAGCACAAGAAAGACGTTATCATTCAGCTCGACTCCATCACTCGTCTGG                                                                        <  SRR4302537.2190944/94‑1 (MQ=60)
                       GTTCAGGTTGCGGAAATGGTGATCGAGAAGGCCAAACGCCTGGATGAGCACAAGAAAGACGTTATCATTCAGCTCGACTCCATCACTCGTCTGG                                                                        >  SRR4302537.3480012/1‑94 (MQ=60)
                        TTCAGGTTGCGGAAATGGTGATCGAGAAGGCCAAACGCCTGGTTGAGCACAAGAAAGACGTTATCATTCAGCTCGACTCCATCACTCGTCTGGC                                                                       >  SRR4302537.4734923/1‑94 (MQ=60)
                        TTCAGGTTGCGGAAATGGTGATCGAGAAGGCCAAACGCCTGGTTGAGCACAAGAAAGACGTTATCATTCAGCTCGACTCCATCACTCGTCTGGC                                                                       <  SRR4302537.3811822/94‑1 (MQ=60)
                        TTCAGGTTGCGGAAATGGTGATCGAGAAGGCCAAACGCCTGGTTGAGCACAAGAAAGACGTTATCATTCAGCTCGACTCCATCACTCGTCTGGC                                                                       <  SRR4302537.65937/94‑1 (MQ=60)
                         TCAGGTTGCGGAAATGGTGATCGAGAAGGCCAAACGCCTGGTTGAGCACAAGAAAGACGTTATCATTCAGCTCGACTCCATCACTCGTCTGGCG                                                                      <  SRR4302537.2585145/94‑1 (MQ=60)
                         TCAGGTTGCGGAAATGGTGATCGAGAAGCCCAAACGCCTGGTTGAGCACAAGAAAGACGTTATCATTCAGCTCGACTCCATCACTCGTCTGGCG                                                                      <  SRR4302537.2456642/94‑1 (MQ=60)
                            GGTTGCGGAAATGGTGATCGAGAAGGCCAAACGCCTGGTTGAGCACAAGAAAGACGTTATCATTCAGCTCGACTCCATCACTCGTCTGGCGCGC                                                                   >  SRR4302537.2337312/1‑94 (MQ=60)
                               TGCGGAAATGGTGATCGAGAAGGCCAAACGCCTGGTTGAGCACAAGAAAGACGTTATCATTCAGCTCGACTCCATCACTCGTCTGGCGCGCGCT                                                                <  SRR4302537.2270813/94‑1 (MQ=60)
                                GCGGAAATGGTGATCGAGAAGGCCAAACGCCTGGTTGAGCACAAGAAAGACGTTATCATTCAGCTCGACTCCATCACTCGTCTGGCGCGCGCTT                                                               <  SRR4302537.1325305/94‑1 (MQ=60)
                                GCGGAAATGGTGATCGAGAAGGCCAAACGCCTGGTTGAGCACAAGAAAGACGTTATCATTCAGCTCGACTCCATCACTCGTCTGGCGCGCGCTT                                                               <  SRR4302537.2517196/94‑1 (MQ=60)
                                 CGGAAATGGTGATCGAGAAGGCCAAACGCCTGGTTGAGCACAAGAAAGACGTTATCATTCAGCTCGACTCCATCACTCGTCTGGCGCGCGCTTA                                                              >  SRR4302537.4721773/1‑94 (MQ=60)
                                   GAAATGGTGATCGAGAAGGCCAAACGCCTGGTTGAGCACAAGAAAGACGTTATCATTCAGCTCGACTCCATCACTCGTCTGGCGCGCGCTTACA                                                            <  SRR4302537.4846547/94‑1 (MQ=60)
                                     AATGGTGATCGAGAAGGCCAAACGCCTGGTTGAGCACAAGAAAGACGTTATCATTCAGCTCGACTCCATCACTCGTCTGGCGCGCGCTTACAAC                                                          <  SRR4302537.4813925/94‑1 (MQ=60)
                                      ATGGTGATCGAGAAGGCCAAACGCCTGGTTGAGCACAAGAAAGACGTTATCATTCAGCTCGACTCCATCACTCGTCTGGCGCGCGCTTACAACA                                                         <  SRR4302537.4457588/94‑1 (MQ=60)
                                       TGGTGATCGAGAAGGCCAAACGCCTGGTTGAGCACAAGAAAGACGTTATCATTCAGCTCGACTCCATCACTCGTCTGGCGCGCGCTTACAACAC                                                        <  SRR4302537.4431777/94‑1 (MQ=60)
                                       TGGTGATCGAGAAGGCCAAACGCCTGGTTGAGCACAAGAAAGACGTTATCATTCAGCTCGACTCCATCACTCGTCTGGCGCGCGCTTACAACAC                                                        >  SRR4302537.3357629/1‑94 (MQ=60)
                                        GGTGATCGAGAAGGCCAAACGCCTGGTTGAGCACAAGAAAGACGTTATCATTCAGCTCGACTCCATCACTCGTCTGGCGCGCGCTTACAACACC                                                       <  SRR4302537.4761777/94‑1 (MQ=60)
                                         GTGATCGAGAAGGCCAAACGCCTGGTTGAGCACAAGAAAGACGTTATCATTCAGCTCGACTCCATCACTCGTCTGGCGCGCGCTTACAACACCG                                                      <  SRR4302537.3876263/94‑1 (MQ=60)
                                         GTGATCGAGAAGGCCAAACGCCTGGTTGAGCAAAAGAAAGGCGTTATCATTCAGCTCGACTCCATCTCTCGTCTGGCGCGCGCTTACAACACCG                                                      <  SRR4302537.3096526/94‑1 (MQ=60)
                                          TGATCGAGAAGGCCAAACGCCTGGTTGAGCACAAGAAAGACGTTATCATTCAGCTCGACTCCATCACTCGTCTGGCGCGCGCTTACAACACCGT                                                     <  SRR4302537.4676904/94‑1 (MQ=60)
                                          TGATCGAGAAGGCCAAACGCCTGGTTGAGCACAAGAAAGACGTTATCATTCAGCTCGACTCCATCACTCGTCTGGCGCGCGCTTACAACACCGT                                                     >  SRR4302537.4068289/1‑94 (MQ=60)
                                          TGATCGAGAAGGCCAAACGCCTGGTTGAGCACAAGAAAGACGTTATCATTCAGCTCGACTCCATCACTCGTCTGGCGCGCGCTTACAACACCGT                                                     >  SRR4302537.3582372/1‑94 (MQ=60)
                                           GATCGAGAAGGCCAAACGCCTGGTTGAGCACAAGAAAGACGTTATCATTCAGCTCGACTCCATCACTCGTCTGGCGCGCGCTTACAACTCCGTT                                                    <  SRR4302537.2226860/94‑1 (MQ=60)
                                            ATCGAGAAGGCCAAACGCCTGGTTGAGCACAAGAAAGACGTTATCATTCAGCTCGACTCCATCACTCGTCTGGCGCGCGCTTACAACACCGTTG                                                   >  SRR4302537.460080/1‑94 (MQ=60)
                                            ATCGAGAAGGCCAAACGCCTGGTTGAGCACAAGAAAGACGTTATCATTCAGCTCGACTCCATCACTCGTCTGGCGCGCGCTTACAACACCGTTG                                                   >  SRR4302537.4884095/1‑94 (MQ=60)
                                                AGAAGGCCAAACGCCTGGTTGAGCACAAGAAAGACGTTATCATTCAGCTCGACTCCATCACTCGTCTGGCGCGCGCTTACAACACCGTTGTTCC                                               >  SRR4302537.4307756/1‑94 (MQ=60)
                                                AGAAGGCCAAACGCCTGGTTGAGCACAAGAAAGACGTTATCATTCAGCTCGACTCCATCACTCGTCTGGCGCGCGCTTACAACACCGTTGTTCC                                               >  SRR4302537.2256225/1‑94 (MQ=60)
                                                AGAAGGCCAAACGCCTGGTTGAGCACAAGAAAGACGTTATCATTCAGCTCGACTCCATCACTCGTCTGGCGCGCGCTTACAACACCGTTGTTCC                                               <  SRR4302537.3090989/94‑1 (MQ=60)
                                                    GGCCAAACGCCTGGTTGAGCACAAGAAAGACGTTATCATTCAGCTCGACTCCATCACTCGTCTGGCGCGCGCTTACAACACCGTTGTTCCGGCG                                           >  SRR4302537.4368398/1‑94 (MQ=60)
                                                    GGCCAAACGCCTGGTTGAGCACAAGAAAGACGTTATCATTCAGCTCGACTCCATCACTCGTCTGGCGCGCGCTTACAACACCGTTGTTCCGGCG                                           >  SRR4302537.2151269/1‑94 (MQ=60)
                                                    GGCCAAACGCCTGGTTGAGCACAAGAAAGACGTTATCATTCAGCTCGACTCCATCACTCGTCTGGCGCGCGCTTACAACACCGTTGTTCCGGCG                                           <  SRR4302537.1319919/94‑1 (MQ=60)
                                                    GGCCAAACGCCTGGTTGAGCACAAGAAAGACGTTATCATTCAGCTCGACTCCATCACTCGTCTGGCGCGCGCTTACAACACCGTTGTTCCGGCG                                           <  SRR4302537.3733110/94‑1 (MQ=60)
                                                     GCCAAACGCCTGGTTGAGCACAAGAAAGACGTTATCATTCAGCTCGACTCCATCACTCGTCTGGCGCGCGCTTACAACACCGTTGTTCCGGCGT                                          >  SRR4302537.1625925/1‑94 (MQ=60)
                                                       CAAACGCCTGGTTGAGCACAAGAAAGACGTTATCATTCAGCTCGACTCCATCACTCGTCTGGCGCGCGCTTACAACACCGTTGTTCCGGCGTCA                                        <  SRR4302537.2659747/94‑1 (MQ=60)
                                                       CAAACGCCTGGTTGAGCACAAGAAAGACGTTATCATTCAGCTCGACTCCATCACTCGTCTGGCGCGCGCTTACAACACCGTTGTTCCGGCGTCA                                        <  SRR4302537.2889335/94‑1 (MQ=60)
                                                       CAAACGCCTGGTTGAGCACAAGAAAGACGTTATCATTCAGCTCGACTCCATCACTCGTCTGGCGCGCGCTTACAACACCGTTGTTCCGGCGTCA                                        >  SRR4302537.2356347/1‑94 (MQ=60)
                                                        AAACGCCTGGTTGAGCACAAGAAAGACGTTATCATTCAGCTCGACTCCATCACTCGTCTGGCGCGCGCTTACAACACCGTTGTTCCGGCGTCAG                                       >  SRR4302537.261854/1‑94 (MQ=60)
                                                        AAACGCCTGGTTGAGCACAAGAAAGACGTTATCATTCAGCTCGACTCCATCACTCGTCTGGCGCGCGCTTACAACACCGTTGTTCCGGCGTCAG                                       <  SRR4302537.3236010/94‑1 (MQ=60)
                                                        AAACGCCTGGTTGAGCACAAGAAAGACGTTATCATTCAGCTCGACTCCATCACTCGTCTGGCGCGCGCTTACAACACCGTTGTTCCGGCGTCAG                                       >  SRR4302537.4508699/1‑94 (MQ=60)
                                                         AACGCCTGGTTGAGCACAAGAAAGACGTTATCATTCAGCTCGAGTCCATCACTCGTCTGGCGCGCGCTTACAACACCGTTGTTCCGGCGTCAGG                                      >  SRR4302537.4569943/1‑94 (MQ=60)
                                                          ACGCCTGGTTGAGCACAAGAAAGACGTTATCATTCAGCTCGACTCCATCACTCGTCTGGCGCGCGCTTACAACACCGTTGTTCCGGCGTCAGGT                                     >  SRR4302537.4368586/1‑94 (MQ=60)
                                                           CGCCTGGTTGAGCACAAGAAAGACGTTATCATTCAGCTCGACTCCATCACTCGTCTGGCGCGCGCTTACAACACCGTTGTTCCGGCGTCAGGTA                                    >  SRR4302537.574879/1‑94 (MQ=60)
                                                           CGCCTGGTTGAGCACAAGAAAGACGTTATCATTCAGCTCGACTCCATCACTCGTCTGGCGCGCGCTTACAACACCGTTGTTCCGGCGTCAGGTA                                    <  SRR4302537.2794427/94‑1 (MQ=60)
                                                           CGCCTGGTTGAGCACAAGAAAGACGTTATCATTCAGCTCGACTCCATCACTCGTCTGGCGCGCGCTTACAACACCGTTGTTCCGGCGTCAGGTA                                    >  SRR4302537.1722672/1‑94 (MQ=60)
                                                             CCTGGTTGAGCACAAGAAAGACGTTATCATTCAGCTCGACTCCATCACTCGTCTGGCGCGCGCTTACAACACCGTTGTTCCGGCGTCAGGTAAA                                  >  SRR4302537.1421785/1‑94 (MQ=60)
                                                             CCTGGTTGAGCACAAGAAAGACGTTATCATTCAGCTCGACTCCATCACTCGTCTGGCGCGCGCTTACAACACCGTTGTTCCGGCGTCAGGTAAA                                  >  SRR4302537.1416043/1‑94 (MQ=60)
                                                              CTGGTTGAGCACAAGAAAGACGTTATCATTCAGCTCGACTCCATCACTCGTCTGGCGCGCGCTTACAACACCGTTGTTCCGGCGTCAGGTAAAG                                 >  SRR4302537.4822785/1‑94 (MQ=60)
                                                              CTGGTTGAGCACAAGAAAGACGCTATCATTCAGCTCGACTCCATCACTCGTCTGGCGCGCGCTTACAACACCGTTGTTCCGGCGTGAGGTAGAG                                 >  SRR4302537.791125/1‑94 (MQ=60)
                                                               TGGTTGAGCACAATAAAGACGTTATCGTTCAGCTCGACTCCATCACTCGTCTGGCTCGCGCTTACAACACCGTTGTTCCGGCGTCAGGTAAAGT                                <  SRR4302537.209036/94‑1 (MQ=60)
                                                               TGGTTGAGCACAAGAAAGACGTTATCATTCAGCTCGACTCCATCACTCGTCTGGCGCGCGCTTACAACACCGTTGTTCCGGCGTCAGGTAAAGT                                <  SRR4302537.4976439/94‑1 (MQ=60)
                                                                GGTTGAGCACAAGAAAGACGTTATCATTCAGCTCGACTCCATCACTCGTCTGGCGCGCGCTTACAACACCGTTGTTCCGGCGTCAGGTAAAGTG                               >  SRR4302537.200467/1‑94 (MQ=60)
                                                                  TTGAGCACAAGAAAGACGTTATCATTCAGCTCGACTCCATCACTCGTCTGGCGCGCGCTTACAACACCGTTGTTCCGGCGTCAGGTAAAGTGTT                             <  SRR4302537.1442354/94‑1 (MQ=60)
                                                                  TTGAGCACAAGAAAGACGTTATCATTCAGCTCGACTCCATCACTCGTCTGGCGCGCGCTTACAACACCGTTGTTCCGGCGTCAGGTAAAGTGTT                             <  SRR4302537.2466744/94‑1 (MQ=60)
                                                                  TTGAGCACAAGAAAGACGTTATCATTCAGCTCGACTCCATCACTCGTCTGGCGCGCGCTTACAACACCGTTGTTCCGGCGTCAGGTAAAGTGTT                             <  SRR4302537.2623248/94‑1 (MQ=60)
                                                                     AGCACAAGAAAGACGTTATCATTCAGCTCGACTCCATCACTCGTCTGGCGCGCGCTTACAACACCGTTGTTCCGGCGTCAGGTAAAGTGTTGAC                          <  SRR4302537.2775279/94‑1 (MQ=60)
                                                                     AGCACAAGAAAGACGTTATCATTCAGCTCGACTCCATCACTCGACTGGCGCGCGCTTACAACACCGTTGTTCCGGCGTCAGGTAAAGTGTTGAC                          >  SRR4302537.3496298/1‑94 (MQ=60)
                                                                      GCACAAGAAAGACGTTATCATTCAGCTGGACTCCATCACTCGTCTGGCGCGCGCTTACAACACCGTTGTTCCGGCGTCAGGTAAAGGGTTGACG                         >  SRR4302537.2889668/1‑94 (MQ=60)
                                                                      GCACAAGAAAGACGTTATCATTCAGCTCGACTCCATCACTCGTCTGGCGCGCGCTTACAACACCGTTGTTCCGGCGTCAGGTAAAGTGTTGACC                         >  SRR4302537.2356240/1‑94 (MQ=60)
                                                                      GCACAAGAAAGACGTTATCATTCAGCTCGACTCCATCACTCGTCTGGCGCGCGCTTAAAACACCGTTGTTCCGGCGTCAGGTAAAGTGTTGACC                         <  SRR4302537.2277506/94‑1 (MQ=60)
                                                                       CACAAGAAAGACGTTATCATTCAGCTCGACTCCATCACTCGTCTGGCGCGCGCTTACAACACCGTTGTTCCGGCGTCAGGTAAAGTGTTGACCG                        <  SRR4302537.6465634/94‑1 (MQ=60)
                                                                       CACAAGAAAGACGTTATCATTCAGCTCGACTCCATCACTCGTCTGGCGCGCGCTTACAACACCGTTGTTCCGGCGTCAGGTAAAGTGTTGACCG                        >  SRR4302537.5426761/1‑94 (MQ=60)
                                                                        ACAAGAAAGACGTTATCATTCAGCTCGACTCCATCACTCGTCTGGCGCGCGCTTACAACACCGTTGTTCCGGCGTCAGGTAAAGTGTTGACCGG                       >  SRR4302537.3208510/1‑94 (MQ=60)
                                                                        ACAAGAAAGACGTTATCATTCAGCTCGACTCCATCACTCGTCTGGCGCGCGCTTACAACACCGTTGTTCCGGCGTCAGGTAAAGTGTTGACCGG                       >  SRR4302537.6452353/1‑94 (MQ=60)
                                                                        ACAAGAAAGACGTTATCATTCAGCTCGACTCCATCACTCGTCTGGCGCGCGCTTACAACACCGTTGTTCCGGCGTCAGGTAAAGTGTTGACCGG                       >  SRR4302537.5034296/1‑94 (MQ=60)
                                                                        ACAAGAAAGACGTTATCATTCAGCTCGACTCCATCACTCGTCTGGCGCGCGCTTACAACACCGTTGTTCCGGCGTCAGGTAAAGTGTTGACCGG                       >  SRR4302537.205872/1‑94 (MQ=60)
                                                                         CAAGAAAGACGTTATCATTCAGCTCGACTCCATCACTCGTCTGGCGCGCGCTTACAACACCGTTGTTCCGGCGTCAGGTAAAGTGTTGACCGGT                      <  SRR4302537.6214185/94‑1 (MQ=60)
                                                                          AAGAAAGACGTTATCATTCAGCTCGACTCCATCACTCGTCTGGCGCGCGCTTACAACACCGTTGTTCCGGCGTCAGGTAAAGTGTTGACCGGTG                     >  SRR4302537.3560651/1‑94 (MQ=60)
                                                                           AGAAAGACGTTATCATTCAGCTCGACTCCATCACTCGTCTGGCGCGCGCTTACAACACCGTTGTTCCGGCGTCAGGTAAAGTGTTGACCGGTGG                    >  SRR4302537.6286464/1‑94 (MQ=60)
                                                                            GAAAGACGTTATAATTCACCTCGCCTCCATCATTCGTCGGGCGCGGGC‑TACAACACCGTTGTTCCGGCGTCAGGTAAAGTGTTGACCGGTGGTG                  <  SRR4302537.4779585/94‑1 (MQ=59)
                                                                             AAAGACGTTATCATTCAGCTCGACTCCATCACTCGTCTGGCGCGCGCTTACAACACCGTTGTTCCGGCGTCAGGTAAAGTGTTGACCGGTGGTG                  <  SRR4302537.5246278/94‑1 (MQ=60)
                                                                               AGACGTTATCATTCAGCTCGACTCCATCACTCGTCTGGCGCGCGCTTACAACACCGTTGTTCCGGCGTCAGGTAAAGTGTTGACCGGTGGTGTG                <  SRR4302537.2287154/94‑1 (MQ=60)
                                                                               AGACGTTATCATTCAGCTCGACTCCATCACTCGTCTGGCGCGCGCTTACAACACCGTTGTTCCGGCGTCAGGTAAAGTGTTGACCGGTGGTGTG                <  SRR4302537.365311/94‑1 (MQ=60)
                                                                                GACGTTATCATTCAGCTCGACTCCATCACTCGTCTGGCGCGCGCTTACAACACCGTTGTTCCGGCGTCAGGTAAAGTGTTGACCGGTGGTGTGG               <  SRR4302537.1830501/94‑1 (MQ=60)
                                                                                GACGTTATCATTCAGCTCGACTCCATCACTCGTCTGGCGCGCGCTTACAACACCGTTGTTCCGGCGTCAGGTAAAGTGTTGACCGGTGGTGTGG               <  SRR4302537.5189102/94‑1 (MQ=60)
                                                                                 ACGTTATCATTCAGCTCGACTCCATCACTCGTCTGGCGCGCGCTTACAACACCGTTGTTCCGGCGTCAGGTAAAGTGTTGACCGGTGGTGTGGA              <  SRR4302537.3364960/94‑1 (MQ=60)
                                                                                  CGTTATCATTCAGCTCGACTCCATCACTCGTCTGGCGCGCGCTTACAACACCGTTGTTCCGGCGTCAGGTAAAGTGTTGACCGGTGGTGTGGAT             <  SRR4302537.1056380/94‑1 (MQ=60)
                                                                                  CGTTATCATTCAGCTCGACTCCATCACTCGTCTGGCGCGCGCTTACAACACCGTTGTTCCGGCGTCAGGTAAAGTGTTGACCGGTGGTGTGGAT             >  SRR4302537.2215839/1‑94 (MQ=60)
                                                                                  CGTTATCATTCAGCTCGACTCCATCACTCGTCTGGCGCGCGCTTACAACACCGTTGTTCCGGCGTCAGGTAAAGTGTTGACCGGTGGTGTGGAT             >  SRR4302537.4091106/1‑94 (MQ=60)
                                                                                  CGTTATCATTCAGCTCGACTCCATCACTCGTCTGGCGCGCGCTTACAACACCGTTGTTCCGGCGTCAGGTAAAGTGTTGACCGGTGGTGTGGAT             >  SRR4302537.5009221/1‑94 (MQ=60)
                                                                                   GTTATCATTCAGCTCGACTCCATCACTCGTCTGGCGCGCGCTTACAACACCGTTGTTCCGGCGTCAGGTAAAGTGTTGACCGGTGGTGTGGATG            >  SRR4302537.609391/1‑94 (MQ=60)
                                                                                   GTTATCATTCAGCTCGACTCCATCACTCGTCTGGCGCGCGCTTACAACACCGTTGTTCCGGCGTCAGGTAAAGTGTTGACCGGCGGTGTGGATG            >  SRR4302537.616927/1‑94 (MQ=60)
                                                                                    TTATCATTCAGCTCGACTCCATCACTCGTCTGGCGCGCGCTTACAACACCGTTGTTCCGGCGTCAGGTAAAGTGTTGACCGGTGGTGTGGATGC           >  SRR4302537.4627537/1‑94 (MQ=60)
                                                                                    TTATCATTCAGCTCGACTCCATCACTCGTCTGGCGCGCGCTTACAACACCGTTGTTCCGGCGTCAGGTAAAGTGTTGACCGGTGGTGTGGATGC           >  SRR4302537.6495544/1‑94 (MQ=60)
                                                                                      ATCATTCAGCTCGACTCCATCACTCGTCTGGCGCGCGCTTACAACACCGTTGTTCCGGCGTCAGGTAAAGTGTTGACCGGTGGTGTGGATGCCA         <  SRR4302537.1240977/94‑1 (MQ=60)
                                                                                      ATCATTCAGCTCGACTCCATCACTCGTCTGGCGCGCGCTTACAACACCGTTGTTCCGGCGTCAGGTAAAGTGTTGACCGGTGGTGTGGATGCCA         >  SRR4302537.1355411/1‑94 (MQ=60)
                                                                                        CATTCAGCTCGACTCCATCACTCGTCTGGCGCGCGCTTACAACACCGTTGTTCCGGCGTCAGGTAAAGTGTTGACCGGTGGTGTGGATGCCAAG       >  SRR4302537.720309/1‑94 (MQ=60)
                                                                                        CATTCAGCTCGACTCCATCACTCGTCTGGCGCGCGCTTACAACACCGTTGTTCCGGCGTCAGGTAAAGTGTTGACCGGTGGTGTGGATGCCAAC       <  SRR4302537.5247421/94‑1 (MQ=60)
                                                                                        CATTCAGCTCGACTCCATCACTCGTCTGGCGCGCGCTTACAACACCGTTGTTCCGGCGTCAGGTAAAGTGTTGACCGGTGGTGTGGATGCCAAC       >  SRR4302537.4754730/1‑94 (MQ=60)
                                                                                         ATTCAGCTCGACTCCATCACTCGTCTGGCGCGCGCTTACAACACCGTTGTTCCGGCGTCAGGTAAAGTGTTGACCGGTGGTGTGGATGGCAACG      >  SRR4302537.2560733/1‑94 (MQ=60)
                                                                                         ATTCAGCTCGACTCCATCACTCGTCTGGCGCGCGCTTACAACACCGTTGTTCCGGCGTCAGGTAAAGTGTTGACCGGTGGTGTGGATGCCACCG      >  SRR4302537.157893/1‑94 (MQ=60)
                                                                                           TCAGCTCGACTCCATCACTCGTCTGGCGCGCGCTTACAACACCGTTGTTCCGGCGTCAGGTAAAGTGTTGACCGGTGGTGTGGATGCCAACGCC    >  SRR4302537.5795292/1‑94 (MQ=60)
                                                                                           TCAGCTCGACTCCATCACTCGTCTGGCGCGCGCTTACAACACCGTTGTTCCGGCGTCAGGTAAAGTGTTGACCGGTGGTGTGGATGCCAACGCC    >  SRR4302537.4541645/1‑94 (MQ=60)
                                                                                           TCAGCTCGACTCCATCACTCGTCTGGCGCGCGCTTACAACACCGTTGTTCCGGCGTCAGGTAAAGTGTTGACCGGTGGTGTGGATGCCAACGCC    <  SRR4302537.2267132/94‑1 (MQ=60)
                                                                                            CAGCTCGACTCCATCACTCGTCTGGCGCGCGCTTACAACACCGTTGTTCCGGCGTCAGGTAAAGTGTTGACCGGTGGTGTGGATGCCAACGCCC   >  SRR4302537.2762031/1‑94 (MQ=60)
                                                                                             AGCTCGACTCCATCACTCGTCTGGCGCGCGCTTACAACACCGTTGTTCCGGCGTCAGGTAAAGTGTTGACCGGTGGTGTGGATGCCAACGCCCT  >  SRR4302537.3991040/1‑94 (MQ=60)
                                                                                             AGCTCGACTCCATCACTCGTCTGGCGCGCGCTTACAACACCGTTGTTCCGGCGTCAGGTAAAGTGTTGACCGGTGGTGTGGATGCCAACGCCCT  <  SRR4302537.3874770/94‑1 (MQ=60)
                                                                                             |                                                                                             
TTGACGAACCCGCATCTCGCCACGTTCAGGTTGCGGAAATGGTGATCGAGAAGGCCAAACGCCTGGTTGAGCACAAGAAAGACGTTATCATTCTGCTCGACTCCATCACTCGTCTGGCGCGCGCTTACAACACCGTTGTTCCGGCGTCAGGTAAAGTGTTGACCGGTGGTGTGGATGCCAACGCCCT  >  NC_000913/3964726‑3964912

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 6 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑