Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A1 F1 I2 R1	882	254.4	12957588	98.0%	12698436	93.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,967,647	(G)_6→7	coding (1005/1050 nt)	wzzE →	putative transport protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,967,641	1	.	G	98.5%	703.0 / 1.3	204	coding (999/1050 nt)	wzzE	putative transport protein
Reads supporting (aligned to +/- strand): ref base . (0/3); new base G (117/84); total (117/87)
Fisher's exact test for biased strand distribution p-value = 7.60e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 253 total aligned reads displayed.

TTTCAGACCTATCGCTATTTGCGTACGCCGGAAGAACCGGTAAAACGCGATAGCCCACGTCGTGCCTTCCTGATGATTATGTGGGGCATTGTC‑GG‑GGGGCTGATCGGGGCTGGTGTCGCATTAACCCGCCGTTGCTCGAAATAGCAACACTGCTGCGGTGAGCGCAAAGGCGCTCGCCGC  >  NC_000913/3967549‑3967728

tttCAGACCTATCGCTATTTGCGTACGCCGGAAGAACCGGTAAAACGCGATAGCCCACGTCGTGCCTTCCTGATGATTATGTGGGGCATTGTc                                                                                           <  2:4462150/93‑1 (MQ=255)

 ttCAGACCTATCGCTATTTGCGTACGCCGGAAGAACCGGTAAAACGCGATAGCCCACGTCGTGCCTTCCTGATGATTATGTGGGGCATTGTC‑g                                                                                         <  1:1363821/93‑1 (MQ=255)

 ttCAGACCTATCGCTATTTGCGTACGCCGGAAGAACCGGTAAAACGCGATAGCCCACGTCGTGCCTTCCTGATGATTATGTGGGGCATTGTC‑g                                                                                         >  2:4077378/1‑93 (MQ=255)

  tCAGACCTATCGCTATTTGCGTACGCCGGAAGAACCGGTAAAACGCGATAGCCCGCGTCGTGCCTTCCTGATGATTATGTGGGGCATTGTC‑gg                                                                                        <  1:3871374/93‑1 (MQ=255)

  tCAGACCTATCGCTATTTGCGTACGCCGGAAGAACCGGTAAAACGCGATAGCCCACGTCGTGCCTTCCTGATGATTATGTGGGGCATTGTC‑gg                                                                                        >  1:3982025/1‑93 (MQ=255)

  tCAGACCTATCGCTATTTGCGTACGCCGGAAGAACCGGTAAAACGCGATAGCCCACGTCGTGCCTTCCTGATGATTATGTGGGGCATTGTC‑gg                                                                                        <  1:2420534/93‑1 (MQ=255)

    aGACCTATCGCTATTTGCGTACGCCGGAAGAACCGGTAAAACGCGATAGCCCACGTCGTGCCTTCCTGATGATTATGTGGGGCATTGTC‑gg‑gg                                                                                     <  1:426881/93‑1 (MQ=255)

    aGACCTATCGCTATTTGCGTACGCCGGAAGAACCGGTAAAACGCGATAGCCCACGTCGTGCCTTCCTGATGATTATGTGGGGCATTGTC‑gg‑gg                                                                                     >  1:5278104/1‑93 (MQ=255)

    aGACCTATCGCTATTTGCGTACGCCGGAAGAACCGGTAAAACGCGATAGCCCACGTCGTGCCTTCCTGATGATTATGTGGGGCATTGTC‑gg‑gg                                                                                     <  1:3071031/93‑1 (MQ=255)

     gACCTATCGCTATTTGCGTACGCCGGAAGAACCGGTAAAACGCGATAGCCCACGTCGTGCCTTCCTGATGATTATGTGGGGCATTGTC‑gg‑ggg                                                                                    <  1:2487659/93‑1 (MQ=255)

     gACCTATCGCTATTTGCGTACGCCGGAAGAACCGGTAAAACGCGATAGCCCACGTCGTGCCTTCCTGATGATTATGTGGGGCATTGTC‑gg‑ggg                                                                                    <  2:4538816/93‑1 (MQ=255)

     aaCCTATCGCTATTTGCGTACGCCGGAAGAACCGGTAAAACGCGATAGCCCACGTCGTGCCTTCCTGATGATTATGTGGGGCATGGTC‑gg‑ggg                                                                                    <  2:6331390/92‑1 (MQ=255)

      aCCTATCGCTATTTGCGTACGCCGGAAGAACCGGTAAAACGCGATAGCCCACGTCGTGCCTTCCTGATGATTATGTGGGGCATTGTC‑gg‑gggg                                                                                   <  1:4377931/93‑1 (MQ=255)

        cTATCGCTATTTGCGTACGCCGGAAGAACCGGTAAAACGCGATAGCCCACGTCGTGCCTTCCTGATGATTATGTGGGGCATTGTC‑gg‑gggggc                                                                                 <  2:4020601/93‑3 (MQ=255)

         tATCGCTATTTGCGTACGCCGGAAGAACCGGTAAAACGCGATAGCCCACGTCGTGCCTTCCTGATGATTATGTGGGGCATTGTC‑gg‑gggggct                                                                                <  2:5781906/93‑4 (MQ=255)

             gCTATTTGCGTACGCCGGAAGAACCGGTAAAACGCGATAGCCCACGTCGTGCCTTCCTGATGATTATGTGGGGCATTGTCGGG‑GGGGCTGATc                                                                             >  2:5736434/1‑93 (MQ=255)

             ccTATTTGCGTACGCGGGAAGAACCGGTAAAACGCGATAGCCCACGTCGTGCCTTCCTGATGATTATGTGGGGCATTGTCGGG‑GGGGCTGATc                                                                             <  2:148271/92‑1 (MQ=255)

              cTATTTGCGTACGCCGGAAGAACCGGTAAAACGCGATAGCCCACGTCGTGCCTTCCTGATGATTATGTGGGGCATTGTCGGG‑GGGGCTGATCg                                                                            <  1:1917735/93‑1 (MQ=255)

               tATTTGCGTACGCCGGAAGAACCGGTAAAACTCGATAGCCCACGTCGTGCCTTCCTGATGATTATGTGGGGCATTGTCGGG‑GGGGCTGATCgg                                                                           <  2:3296258/93‑1 (MQ=255)

               tATTTGCGTACGCCGGAAGAACCGGTAAAACGCGATAGCCCACGTCGTGCCTTCCTGATGATTATGTGGGGCATTGTCGGG‑GGGGCTGATCgg                                                                           <  2:6224174/93‑1 (MQ=255)

               tATTTGCGTACGCCGGAAGAACCGGTAAAACGCGATAGCCCACGTCGTGCCTTCCTGATGATTATGTGGGGCATTGTCGGG‑GGGGCTGATCgg                                                                           >  2:352576/1‑93 (MQ=255)

               tATTTGCGTACGCCGGAAGAACCGGTAAAACGCGATAGCCCACGTCGTGCCTTCCTGATGATTATGTGGGGCATTGTCGGG‑GGGGCTGATCgg                                                                           <  2:1136719/93‑1 (MQ=255)

                aTTTGCGTACGCCGGAAGAACCGGTAAAACGCGATAGCCCACGTCGTGCCTTCCTGATGATTATGTGGGGCATTGTCGGG‑GGGGCTGATCggg                                                                          <  2:1396236/93‑1 (MQ=255)

                aTTTGCGTACGCCGGAAGAACCGGTAAAACGCGATAGCCCACGTCGTGCCTTCCTGATGATTATGTGGGGCATTGTCGGG‑GGGGCTGATCggg                                                                          <  2:2233177/93‑1 (MQ=255)

                 tttGCGTACGCCGGAAGAACCGGTAAAACGCGATAGCCCACGTCGTGCCTTCCTGATGATTATGTGGGGCATTGTCGGG‑GGGGCTGATCgggg                                                                         >  2:1018349/1‑93 (MQ=255)

                 tttGCGTACGCCGGAAGAACCGGTAAAACGCGATAGCCCACGTCGTGCCTTCCTGATGATTATGTGGGGCATTGTCGGG‑GGGGCTGATCgggg                                                                         <  2:3697425/93‑1 (MQ=255)

                 tttGCGTACGCCGGAAGAACCGGTAAAACGCGATAGCCCACGTCGTGCCTTCCTGATGATTATGTGGGGCATTGTCGGG‑GGGGCTGATCgggg                                                                         >  1:1445313/1‑93 (MQ=255)

                 tttGCGTACGCCGGAAGAACCGGTAAAACGCGATAGCCCACGTCGTGCCTTCCTGATGATTATGTGGGGCATTGTCGGG‑GGGGCTGATCgggg                                                                         >  1:4230919/1‑93 (MQ=255)

                   tGCGTACGCCGGAAGAACCGGTAAAACGCGATAGCCCACGTCGTGCCTTCCTGATGATTATGTGGGGCATTGTCGGG‑GGGGCTGATCGGGGCt                                                                       >  1:371329/1‑93 (MQ=255)

                    gCGTACGCCGGAAGAACCGGTAAAACGCGATAGCCCACGTCGTGCCTTCCTGATGATTATGTGGGGCATTGTCGGG‑GGGGCTGATCGGGGCTg                                                                      <  2:5612770/93‑1 (MQ=255)

                     cGTACGCCGGAAGAACCGGTAAAACGCGATAGCCCACGTCGTGCCTTCCTGATGATTATGTGGGGCATTGTCGGG‑GGGGCTGATCGGGGCTgg                                                                     <  1:1211318/93‑1 (MQ=255)

                      gTACGCCGGAAGAACCGGTAAAACGCGATAGCCCACGTCGTGCCTTCCTGATGATTATGTGGGGCATTGTCGGG‑GGGGCTGATCGGGGCTGgt                                                                    >  2:4847021/1‑93 (MQ=255)

                      gTACGCCGGAAGAACCGGTAAAACGCGATAGCCCACGTCGTGCCTTCCTGATGATTATGTGGGGCATTGTCGGG‑GGGGCTGATCGGGGCTGgt                                                                    <  2:764394/93‑1 (MQ=255)

                      gTACGCCGGAAGAACCGGTAAAACGCGATAGCCCACGTCGTGCCTTCCTGATGATTATGTGGGGCATTGTCGGG‑GGGGCTGATCGGGGCTGgt                                                                    >  1:4979227/1‑93 (MQ=255)

                        tcGCCGGAAGAACCGGTAAAACGCGATAGCCCACGTCGTGCCTTCCTGATGATTATGTGGGGCATTGTCGGG‑GGGGCTGATCGGGGCTGgtgt                                                                  <  1:189914/92‑1 (MQ=255)

                        aCGCCGGAAGAACCGGTAAAACGCGATAGCCCACGTCGTGCCTTCCTGATGATTATGTGGGGCATTGTCGGG‑GGGGCTGATCGGGGCTGgtgt                                                                  <  2:2722463/93‑1 (MQ=255)

                            cGGAAGAACCGGTAACACGCGATAGCCTACGGCGTGCCTTCCTGATGATTATGTGGGGCATTGTC‑GGTGGGGCTGATCGGGGCTGGTGTCGCa                                                              <  2:4287935/93‑1 (MQ=255)

                             ggAAGAACCGGTAAAACGCGATAGCCCACGTCGTGCCTTCCTGATGATTATGTGGGGCATTGTCGGG‑GGGGCTGATCGGGGCTGGTGTCGCAt                                                             >  2:1706394/1‑93 (MQ=255)

                              gaagaaCCGGTAAAACGCGATAGCCCACGTCGTGCCTTCCTGATGATTATGTGGGGCATTGTCGGG‑GGGGCTGATCGGGGCTGGTGTCGCAtt                                                            >  1:2341367/1‑93 (MQ=255)

                              gaagaaCCGGTAAAACGCGATAGCCCACGTCGTGCCTTCCTGATGATTATGTGGGGCATTGTCGGG‑GGGGCTGATCGGGGCTGGTGTCGCAtt                                                            >  2:2460919/1‑93 (MQ=255)

                                agaaCCGGTAAAACGCGATAGCCCACGTCGTGCCTTCCTGATGATTATGTGGGGCATTGTCGGG‑GGGGCTGATCGGGGCTGGTGTCGCATTaa                                                          >  2:3107480/1‑93 (MQ=255)

                                 gaaCCGGTAAAACGCGATAGACCACGTCGTGCCTTCCTGATGATTATGTGGGGCATTGTCGGG‑GGGGCTGATCGGGGCTGGTGTCGCATTAAc                                                         >  1:4921917/1‑93 (MQ=255)

                                  aaCCGGTAAAACGCGATAGCCCACGTCGTGCCTTCCTGATGATTATGTGGGGCATTGTCGGG‑GGGGCTGATCGGGGCTGGTGTCGCATTAAcc                                                        >  1:2704258/1‑93 (MQ=255)

                                  aaCCGGTAAAACGCGATAGCCCACGTCGTGCCTTCCTGATGATTATGTGGGGCATTGTCGGG‑GGGGCTGATCGGGGCTGGTGTCGCATTAAcc                                                        <  2:519990/93‑1 (MQ=255)

                                   aCCGGTAAAACGCGATAGCCCACGTCGTGCCTTCCTGATGATTATGTGGGGCATTGTCGGG‑GGGGCTGATCGGGGCTGGTGTCGCGTTAAccc                                                       >  1:3607272/1‑93 (MQ=255)

                                     cGGTAAAACGCGATAGCCCACGTCGTGCCTTCCTGATGATTATGTGGGGCATTGTCGGG‑GGGGCTGATCGGGGCTGGTGTCGCATTAACccgc                                                     <  2:4990834/93‑1 (MQ=255)

                                      ggTAAAACGCGATAGCCCACGTCGTGCCTTCCTGATGATTATGTGGGGCATTGTCGGG‑GGGGCTGATCGGGGCTGGTGTCGCATTAACccgcc                                                    >  2:3447063/1‑93 (MQ=255)

                                      ggTAAAACGCGATAGCCCACGTCGTGCCTTCCTGATGATTATGTGGGGCATTGTCGGG‑GGGGCTGATCGGGGCTGGTGTCGCATTAACccgcc                                                    >  1:4643932/1‑93 (MQ=255)

                                      ggTAAAACGCGATAGCCCACGTCGTGCCTTCCTGATGATTATGTGGGGCATTGTCGGG‑GGGGCTGATCGGGGCTGGTGTCGCATTAACccgcc                                                    >  1:1016596/1‑93 (MQ=255)

                                        tAAAACGCGATAGCCCACGTCGTGCCTTCCTGATGATTATGTGGGGCATTGTCGGG‑GGGGCTGATCGGGGCTGGTGTCGCATTAACCCGCCGt                                                  >  1:4881267/1‑93 (MQ=255)

                                        tAAAACGCGATAGCCCACGTCGTGCCTTCCTGATGATTATGTGGGGCATTGTCGGG‑GGGGCTGATCGGGGCTGGTGTCGCATTAACCCGCCGt                                                  >  2:3020933/1‑93 (MQ=255)

                                         aaaaCGCGATAGCCCACGTCGTGCCTTCCTGATGATTATGTGGGGCATTGTCGGG‑GGGGCTGATCGGGGCTGGTGTCGCATTAACCCGCCGtt                                                 <  2:5452431/93‑1 (MQ=255)

                                         aaaaCGCGATAGCCCACGTCGTGCCTTCCTGATGATTATGTGGGGCATTGTCGGG‑GGGGCTGATCGGGGCTGGTGTCGCATTAACCCGCCGtt                                                 >  1:3821812/1‑93 (MQ=255)

                                           aaCGCGATAGCCCACGTCGTGCCTTCCTGATGATTATGTGGGGCATTGTCGGG‑GGGGCTGATCGGGGCTGGTGTCGCATTAACCCGCCGTTGc                                               >  1:3507749/1‑93 (MQ=255)

                                            aCGCGATAGCCCACGTCGTGCCTTCCTGATGATTATGTGTGGCATTGTCGGG‑GGGGCTGATCGGGTCTGGTGTCGCATTAACCCGCCGTTGCt                                              <  1:2278368/93‑1 (MQ=255)

                                             cgcgATAGCCCACGTCGTGCCTTCCTGATGATTATGTGGGGCATTGTCGGG‑GGGGCTGATCGGGGCTGGTGTCGCATTAACCCGCCGTTGCTc                                             <  1:4778411/93‑1 (MQ=255)

                                              gcgATAGCCCACGTCGTGCCTTCCTGATGATTATGTGGGGCATTGTCGGG‑GGGGCTGCTCGGGGCTGGTGTCGCATTAACCCGCCGTTGCTCg                                            >  1:6451384/1‑93 (MQ=255)

                                                 aTAGCCCACGTCGTGCCTTCCTGATGATTATGTGGGGCATTGTCGGG‑GGGGCTGATCGGGGCTGGTGTCGCATTAACCCGCCGTTGCTCGaaa                                         >  1:3205753/1‑93 (MQ=255)

                                                  tAGCCCACGTCGTGCCTTCCTGATGATTATGTGGGGCATTGTCGGG‑GGGGCTGATCGGGGCTGGTGTCGCATTAACCCGCCGTTGCTCGAAAt                                        <  2:3220946/93‑1 (MQ=255)

                                                   aGCCCACGTCGTGCCTTCCTGATGATTATGTGGGGCATTGTCGGG‑GGGGCTGATCGGGGCTGGTGTCGCATTAACCCGCCGTTGCTCGAAATa                                       <  1:5206361/93‑1 (MQ=255)

                                                   aGCCCACGTCGTGCCTTCCTGATGATTATGTGGGGCATTGTCGGG‑GGGGCTGATCGGGGCTGGTGTCGCATTAACCCGCCGTTGCTCGAAATa                                       >  2:2009838/1‑93 (MQ=255)

                                                     cccACGTCGTGCCTTCCTGATGATTATGTGGGGCATTGTCGGG‑GGGGCTGATCGGGGCTGGTGTCGCATTAACCCGCCGTTGCTCGAAATAGc                                     <  1:3733734/93‑1 (MQ=255)

                                                     cccACGTCGTGCCTTCCTGATGATTATGTGGGGCATTGTCGGG‑GGGGCTGATCGGGGCTGGTGTCGCATTAACCCGCCGTTGCTCGAAATAGc                                     >  1:2217508/1‑93 (MQ=255)

                                                      ccACGTCGTGCCTTCCTGATGATTATGTGGGGCATTGTCGGG‑GGGGCTGATCGGGGCTGGTGTCGCATTAACCCGCCGTTGCTCGAAATAGCa                                    >  1:5095890/1‑93 (MQ=255)

                                                       cACGTCGTGCCTTCCTGATGATTATGTGGGGCATTGTCGGG‑GGGGCTGATCGGGGCTGGTGTCGCATTAACCCGCCGTTGCTCGAAATAGCaa                                   >  2:6089720/1‑93 (MQ=255)

                                                        ccgtcgtGCCTTCCTGATGATTATGTGGGCCATTGTCGGG‑GGGGCTGATCGGGGCTGGTGTCGCATTAACCCGCCGTTGCTCGAAATAGCAac                                  <  1:2529413/92‑1 (MQ=255)

                                                        aCGTCGTGCCTTCCTGATGATTATGTGGTGCATTGTCGGG‑GGGGCTGATCGGGGCTGGTGTCGCATTACCCCGCCGTTGCTCGAAATAGCAac                                  <  2:6159976/93‑1 (MQ=255)

                                                         cgtcgtGCCTTCCTGATGATTATGTGGGGCATTGTCGGG‑GGGGCTGATCGGGGCTGGTGTCGCATTAACCCGCCGTTGCTCGAAATAGCAaca                                 >  2:5258550/1‑93 (MQ=255)

                                                           tcgtGCCTTCCTGATGATTATGTGGGGCATTGTCGGG‑GGGGCTGATCGGGGCTGGTGTCGCATTAACCCGCCGTTGCTCGAAATAGCAACAct                               >  1:5670512/1‑93 (MQ=255)

                                                           tcgtGCCTTCCTGATGATTATGTGGGGCATTGTCGGG‑GGGGCTGATCGGGGCTGGTGTCGCATTAACCCGCCGTTGCTCGAAATAGCAACAct                               >  1:2108534/1‑93 (MQ=255)

                                                              tGCCTTCCTGATGATTATGTGGGGCATTGTCGGG‑GGGGCTGATCGGGGCTGGTGTCGCATTAACCCGCCGTTGCTCGAAATAGCAACActgct                            >  1:5078566/1‑93 (MQ=255)

                                                              tGCCTTCCTGATGATTATGTGGGGCATTGTCGGG‑GGGGCTGATCGGGGCTGGTGTCGCATTAACCCGCCGTTGCTCGAAATAGCAACActgct                            >  2:4269596/1‑93 (MQ=255)

                                                              tGCCTTCCTGATGATTATGTGGGGCATTGTCGGG‑GGGGCTGATCGGGGCTGGTGTCGCATTAACCCGCCGTTGCTCGAAATAGCAACActgct                            <  1:4767560/93‑1 (MQ=255)

                                                              tGCCTTCCTGATGATTATGTGGGGCATTGTCGGG‑GGGGCTGATCGGGGCTGGTGTCGCATTAACCCGCCGTTGCTCGAAATAGCAACActgct                            >  2:2578044/1‑93 (MQ=255)

                                                                ccTTCCTGATGATTATGTGGGGCATTGTCGGG‑GGGGCTGATCGGGGCTGGTGTCGCATTAACCCGCCGTTGCTCGAAATAGCAACACtgctgc                          >  1:1581875/1‑93 (MQ=255)

                                                                 cTTCCTGATGATTATGTGGGGCATTGTCGGG‑GGGGCTGATCGGGGCTGGTGTCGCATTAACCCGCCGTTGCTCGAAATAGCAACACTGCTGCg                         >  2:6448614/1‑93 (MQ=255)

                                                                  ttCCTGATGATTATGTGGGGCATTGTCGGG‑GGGGCTGATCGGGGCTGGTGTCGCATTAACCCGCCGTTGCTCGAAATAGCAACACtgctgcag                        >  1:2759934/1‑91 (MQ=255)

                                                                  ttCCTGATGATTATGTGGGGCATTGTCGGG‑GGGGCTGATCGGGGCTGGTGTCGCATTAACCCGCCGTTGCTCGAAATAGCAACACTGCTGCgg                        >  2:884010/1‑93 (MQ=255)

                                                                  ttCCTGATGATTATGTGGGGCATTGTCGGG‑GGGGCTGATCGGGGCTGGTGTCGCATTAACCCGCCGTTGCTCGAAATAGCAACACTGCTGCgg                        >  1:5853216/1‑93 (MQ=255)

                                                                  ttCCTGATGATTATGTGGGGCATTGTCGGG‑GGGGCTGATCGGGGCTGGTGTCGCATTAACCCGCCGTTGCTCGAAATAGCAACACTGCTGCgg                        >  2:1300045/1‑93 (MQ=255)

                                                                  ttCCTGATGATTATGTGGGGCATTGTCGGG‑GGGGCTGATCGGGGCTGGTGTCGCATTAACCCGCCGTTGCTCGAAATAGCAACACTGCTGCgg                        >  2:1602678/1‑93 (MQ=255)

                                                                  ttCCTGATGATTATATGGGGCATTGTCGGG‑GGGGCTGATCGGGGCTGGTGTCGCATTAACCCGCCGTTGCTCGAAATAGAAACACTGCTGCgg                        >  2:3941654/1‑93 (MQ=255)

                                                                   tCCTGATGATTATGTGGGGCATTGTCGGG‑GGGGCTGATCGGGGCTGGTGTCGCATTAACCCGCCGTTGCTCGAAATAGCAACACTGCTGCGGt                       >  1:5429392/1‑93 (MQ=255)

                                                                   tCCTGATGATTATGTGGGGCATTGTCGGG‑GGGGCTGATCGGGGCTGGTGTCGCATTAACCCGCCGTTGCTCGAAATAGCAAAACTGCTGCGGt                       >  2:1434242/1‑93 (MQ=255)

                                                                    ccTGATGATTATGTGGGGCATTGTCGGG‑GGGGCTGATCGGGGCTGGTGTCGCATTAACCCGCCGTTGCTCGAAATAGCAACACTGCTGCGGTg                      >  2:2404508/1‑93 (MQ=255)

                                                                     cTGATGATTATGTGGGGCATTGTCGGG‑GGGGCTGATCGGGGCTGGTGTCGCATTAACCCGCCGTTGCTCGAAATAGCAACACTGCTGCGGTGa                     >  1:2065016/1‑93 (MQ=255)

                                                                     cTGATGATTATGTGGGGCATTGTCGGG‑GGGGCTGATCGGGGCTGGTGTCGCATTAACCCGCCGTTGCTCGAAATAGCAACACTGCTGCGGTGa                     >  1:4558692/1‑93 (MQ=255)

                                                                        atgatTATGTGGGGCATTGTCGGG‑GGGGCTGATCGGGGCTGGTGTAGCATTAACCCGCCGTTGCTCGAAATAGCAACACTGCTGCGGTGAgcg                  >  2:2115682/1‑93 (MQ=255)

                                                                           atTATGTGGGGCATTGTCGGG‑GGGGCTGATCGGGGCTGGTGTCGCATTAACCCGCCGTTGCTCGAAATAGCAACACTGCTGCGGTGAGCGCaa               <  1:3486464/93‑1 (MQ=255)

                                                                            ttATGGGGGGCATTGTCGGG‑GGGGCTGATCGGGGCTGGTGTCGCATTACCCCGCCGTTGCTCGAAATAGCAACACTGCTGCGGTGAGCGCaaa              <  2:4621252/93‑1 (MQ=255)

                                                                             tATGTGGGGCATTGTCGGG‑GGGGCTGATCGGGGCTGGTGTCGCATTAACCCGCCGTTGCTCGAAATAGCAACACTGCTGCGGTGAGCGCAAAg             >  2:5926683/1‑93 (MQ=255)

                                                                              aTGTGGGGCATTGTCGGG‑GGGGCTGATCGGGGCTGGTGTCGCATTACCCCGCCGTTGCTCGAAATAGCAACACTGCTGCGGTGAGCGCAAAgg            <  2:378165/93‑1 (MQ=255)

                                                                                gtgGGGCATTGTCGGG‑GGGGCTGATCGGGGCTGGTGTCGCATTAACCCGCCGTTGCTCGAAATAGCAACACTGCTGCGGTGAGCGCAAAGgcg          <  1:3697957/93‑1 (MQ=255)

                                                                                 tgGGGCATTGTCGGG‑GGGGCTGATCGGGGCTGGTGTCGCATTAACCCGCCGTTGCTCGAAATAGCAACACTGCTGCGGTGAGCGCAAAGgcgc         <  2:219562/93‑1 (MQ=255)

                                                                                 tgGGGCATTGTCGGG‑GGGGCTGATCGGGGCTGGTGTCGCATTAACCCGCCGTTGCTCGAAATAGCAACACTGCTGCGGTGAGCGCAAAGgcgc         >  2:5354139/1‑93 (MQ=255)

                                                                                    ggCATTGTCGGG‑GGGGCTGATCGGGGCTGGTGTCGCATTAACCCGCCGTTGCTCGAAATAGCAACACTGCTGCGGTGAGCGCAAAGGCGCTcg      >  2:6391763/1‑93 (MQ=255)

                                                                                     gCATTGTCGGG‑GGGGCTGATCTGGGCTGGTGTCGCATTAACCCGCCGTTGCTCGAAATAGCAACACTGCTGCGGTGAGCGCAAAGGCGCTcgc     >  1:6142066/1‑93 (MQ=255)

                                                                                      cATTGTCGGG‑GGGGCTGATCGGGGCTGGTGTCGCATTAACCCGCCGTTGCTCGAAATAGCAAAACTGCTGCGGTGAGCGCAAAGGCGCTcgcc    >  1:1797862/1‑93 (MQ=255)

                                                                                        ttGTCGGG‑GGGGCTGATCGGGGCTGGTGTCGCATTAACCCGCCGTTGCTCGAAATAGCAACACTGCTGCGGTGAGCGCAAAGGCGCTcgccgc  >  2:4306136/1‑93 (MQ=255)

                                                                                        ttGTCGGG‑GGGGCTGATCGGGGCTGGTGTCGCATTAACCCGCCGTTGCTCGAAATAGCAACACTGCTGCGGTGAGCGCAAAGGCGCTcgccgc  >  1:91061/1‑93 (MQ=255)

TTTCAGACCTATCGCTATTTGCGTACGCCGGAAGAACCGGTAAAACGCGATAGCCCACGTCGTGCCTTCCTGATGATTATGTGGGGCATTGTC‑GG‑GGGGCTGATCGGGGCTGGTGTCGCATTAACCCGCCGTTGCTCGAAATAGCAACACTGCTGCGGTGAGCGCAAAGGCGCTCGCCGC  >  NC_000913/3967549‑3967728

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 18 ≤` `ATCG/ATCG` `< 26 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

BRESEQ :: bam2aln output

GACCTATCGCTATTTGCGTACGCCGGAAGAACCGGTAAAACGCGATAGCCCACGTCGTGCCTTCCTGATGATTATGTGGGGCATTGT‑C‑GGGG‑GG‑CTGATCGGGGCTGGTGTCGCATTAACCCGCCGTTGCTCGAAATAGCAACACTGCTGCGGTGAGCGCAAAGGCGCTCGCCGCTTATTCGAAGA  >  NC_000913/3967554‑3967739

GACCTATCGCTATTTGCGTACGCCGGAAGAACCGGTAAAACGCGATAGCCCACGTCGTGCCTTCCTGATGATTATGTGGGGCATTGT‑C‑GGGG‑GG                                                                                               <  SRR4302537.2505179/94‑1 (MQ=60)

GACCTATCGCTATTTGCGTACGCCGGAAGAACCGGTAAAACGCGATAGCCCACGTCGTGCCTTCCTGATGATTATGTGGGGCATTGT‑C‑GGGG‑GG                                                                                               >  SRR4302537.461475/1‑94 (MQ=60)

 ACCTATCGCTATTTGCGTACGCCGGAAGAACCGGTAAAACGCGATAGCCCACGTCGTGCCTTCCTGATGATTATGTGGGGCATTGT‑C‑GGGG‑GG‑G                                                                                             <  SRR4302537.4408158/94‑1 (MQ=60)

  CCTATCGCTATTTGCGTACGCCGGAAGAACCGGTAAAACGCGATAGCCCACGTCGTGCCTTCCTGATGATTATGTGGGGCATTGT‑C‑GGGG‑GGgc                                                                                             >  SRR4302537.536471/1‑92 (MQ=60)

  CCTATCGCTATTTGCGTACGCCGGAAGAACCGGTAAAACGCGATAGCCCACGTCGTGCCTTCCTGATGATTATGTGGGGCATTGT‑C‑GGGG‑GGgc                                                                                             >  SRR4302537.2574293/1‑92 (MQ=60)

  CCTATCGCTATTTGCGTACGCCGGAAGAACCGGTAAAACGCGATAGCCCACGTCGTGCCTTCCTGATGATTATGTGGGGCATTGT‑C‑GGGG‑GGgc                                                                                             >  SRR4302537.1904745/1‑92 (MQ=60)

   CTATCGCTATTTGCGTACGCCGGAAGAACCGGTAAAACGCGATAGCCCACGTCGTGCCTTCCTGATGATTATGTGGGGCATTGT‑C‑GGGG‑GGgct                                                                                            >  SRR4302537.6271570/1‑91 (MQ=60)

   CTATCGCTATTTGCGTACGCCGGAAGAACCGGTAAAACGCGATAGCCCACGTCGTGCCTTCCTGATGATTATGTGGGGCATTGT‑C‑GGGG‑GGgct                                                                                            >  SRR4302537.2533304/1‑91 (MQ=60)

     ATCGCTATTTGCGTACGCCGGAAGAACCGGTAAAACGCGATAGCCCACGTCGTGCCTTCCTGATGATTATGTGGGGCATTGT‑CGGGGG‑GG‑CTGA                                                                                          >  SRR4302537.2047589/1‑94 (MQ=60)

      TCGCTATTTGCGTACGCCGGAAGAACCGGTAAAATGCGATAGCCCACTTCGTGCCTTCCTGATGATTATGTGGGGCATTGT‑CGGGGG‑GG‑CTGAT                                                                                         <  SRR4302537.4375809/94‑1 (MQ=60)

      TCGCTATTTGCGTACGCCGGAAGAACCGGTAAAACGCGATAGCCCACGTCGTGCCTTCCTGATGATTATGTGGGGCATTGT‑CGGGGG‑GG‑CTGAT                                                                                         <  SRR4302537.4412999/94‑1 (MQ=60)

       CGCTATTTGGGTACGCCGGAAGAACCGGTAAAACGCGATAGCCCACGTCGTGCCTTCCTGATGATTATGTGGGGCATTGT‑CGGGGG‑GG‑CTGATC                                                                                        <  SRR4302537.4126167/94‑1 (MQ=60)

       CGCTATTTGCGTACGCCGGAAGAACCGGTAAAACGCGATAGCCCACGTCGTGCCTTCCTGATGATTATGTGGGGCATTGT‑CGGGGG‑GG‑CTGATC                                                                                        <  SRR4302537.4079034/94‑1 (MQ=60)

       CGCTATTTGCGTACGCCGGAAGAACCGGTAAAACGCGATAGCCCACGTCGTGCCTTCCTGATGATTATGTGGGGCATTGT‑CGGGGG‑GG‑CTGATC                                                                                        >  SRR4302537.5181745/1‑94 (MQ=60)

        GCTATTTGCGTACGCCGGAAGAACCGGTAAAACGCGCTAGCCCACGTCGTGCCTTCCTGATGATTATGTGGGGCATTGT‑C‑GGGGCGG‑CTGCTCG                                                                                       >  SRR4302537.3671418/1‑94 (MQ=60)

         CTATTTGCGTACGCCGGAAGAACCGGTAAAACGCGATAGCCCACGTCGTGCCTTCCTGATGATTATGTGGGGCATTGT‑CGGGGG‑GG‑CTGATCGG                                                                                      <  SRR4302537.1931050/94‑1 (MQ=60)

         CTATTTGCGTACGCCGGAAGAACCGGTAAAACGCGATAGCCCACGTCGTGCCTTCCTGATGATTATGTGGGGCATTGT‑CGGGGG‑GG‑CTGATCGG                                                                                      >  SRR4302537.225077/1‑94 (MQ=60)

          TATTTGCGTACGCCGGAAGAACCGGTAAAACGCGATAGCCCACGTCGTGCCTTCCTGATGATTATGTGGGGCATTGT‑CGGGGG‑GG‑CTGATCGGG                                                                                     >  SRR4302537.4282196/1‑94 (MQ=60)

          TATTTGCGTACGCCGGAAGAACCGGTAAAACGCGATAGCCCACGTCGTGCCTTCCTGATGATTATGTGGGGCATTGT‑CGGGGG‑GG‑CTGATCGGG                                                                                     <  SRR4302537.2842609/94‑1 (MQ=60)

           ATTTGCGTACGCCGGAAGAACCGGTAAAACGCGATAGCCCACGTTGTGCCTTCCTGATGATTATGTGGGGCATTGT‑CGGGGG‑GG‑CTGATCGGGG                                                                                    >  SRR4302537.2133896/1‑94 (MQ=60)

           ATTTGCGTACGCCGGAAGAACCGGTAAAACGCGATAGCCCACGTCGTGCCTTCCTGATGATTATGTGGGGCATTGT‑CGGGGG‑GG‑CTGATCGGGG                                                                                    >  SRR4302537.4260372/1‑94 (MQ=60)

           ATTTGCGTACGCCGGAAGAACCGGTAAAACGCGATAGCCCACGTCGTGCCTTCCTGATGATTATGTGGGGCATTGT‑CGGGGG‑GG‑CTGATCGGGG                                                                                    <  SRR4302537.3755528/94‑1 (MQ=60)

           ATTTGCGTACGCCGGAAGAACCGGTAAAACGCGATAGCCCACGTCGTGCCTTCCTGATGATTATGTGGGGCATTGT‑CGGGGG‑GG‑CTGATCGGGG                                                                                    >  SRR4302537.3739006/1‑94 (MQ=60)

           ATTTGCGTACGCCGGAAGAACCGGTAAAACGCGATAGCCCACGTCGTGCCTTCCTGATGATTATGTGGGGCATTGT‑CGGGGG‑GG‑CTGATCGGGG                                                                                    >  SRR4302537.1455384/1‑94 (MQ=60)

             TTGCGTACGCCGGAAGAACCGGTAAAACGCGATAGCCCACGTCGTGCCTTCCTGATGATTATGTGGGGCATTGT‑CGGGGG‑GG‑CTGATCGGGGCT                                                                                  >  SRR4302537.373902/1‑94 (MQ=60)

             TGGCGTACGCCGGAAGAACCGGTAAAACGCGATAGCCCACGTCGTGCCTTCCTGATGATTATGTGGGGCATTGT‑CGGGGG‑GG‑CTGATCGGGGCT                                                                                  <  SRR4302537.4806633/94‑1 (MQ=60)

                CGTACGCCGGAAGAACCGGTAAAACGCGATAGCCCACGTCGTGCCTTCCTGATGATTATGTGGGGCATTGT‑CGGGGG‑GG‑CTGATCGGGGCTGGT                                                                               <  SRR4302537.918813/94‑1 (MQ=60)

                CGTACGCCGGAAGAACCGGTAAAACGCGATAGCCCACGTCGTGCCTTCCTGATGATTATGTGGGGCATTGT‑CGGGGG‑GG‑CTGATCGGGGCTGGT                                                                               >  SRR4302537.5013545/1‑94 (MQ=60)

                CGTACGCCGGAAGAACCGGTAAAACGCGATAGCCCACGTCGTGCCTTCCTGATGATTATGTGGGGCATTGT‑CGGGGG‑GG‑CTGATCGGGGCTGGT                                                                               <  SRR4302537.1219745/94‑1 (MQ=60)

                 GTACGCCGGAAGAACCGGTAAAACGCGATAGCCCACGTCGTGCCTTCCTGATGATTATGTGGGGCATTGT‑CGGGGG‑GG‑CTGATCGGGGCTGGTG                                                                              <  SRR4302537.799919/94‑1 (MQ=60)

                 GTACGCCGGAAGAACCGGTAAAACGCGATAGCCCACGTCGTGCCTTCCTGATGATTATGTGGGGCATTGT‑CGGGGG‑GG‑CTGATAGGGGCTGGTG                                                                              <  SRR4302537.3839637/94‑1 (MQ=60)

                  TACGCCGGAAGAACCGGTAAAACGCGATAGCCCACGTCGTGCCTTCCTGATGATTATGTGGGGCATTGT‑CGGGGG‑GG‑CTGATCGGGGCTGGTGT                                                                             >  SRR4302537.1917964/1‑94 (MQ=60)

                   TCGCCGGAAGAACCGGTAAAACGCGATAGCCCACGTCGTGCCTTCCTGATGATTATGTGGGGCATTGT‑CGGGGG‑GG‑CTGATCGGGGCTGGTGTC                                                                            <  SRR4302537.191266/94‑1 (MQ=60)

                     GCCGGAAGAACCGGTAAAACGCGATAGCCCACGTCGTGCCTTCCTGATGATTATGTGGGGCATTGT‑C‑GGGG‑GGTCTGATCGGGGCTGGgggcgt                                                                          >  SRR4302537.4337285/1‑88 (MQ=60)

                      CCGGAAGAACCGGTAAAACGCGATAGCCCACGTCGTGCCTTCCTGATGATTATGTGGGGCATTGT‑CGGGGG‑GG‑CTGATCGGGGCTGGTGTCGCA                                                                         >  SRR4302537.374201/1‑94 (MQ=60)

                      CCGGAAGAACCGGTAAAACGCGATAGCCCACGTCGTGCCTTCCTGATGATTATGTGGGGCATTGT‑CGGGGG‑GG‑CTGATCGGGGCTGGTGGCGCA                                                                         >  SRR4302537.1330961/1‑94 (MQ=60)

                        GGAAGAACCGGTAAAACGCGATAGCCCACGTCGTGCCTTCCTGATGATTATGTGGGGCATTGT‑CGGGGG‑GG‑CTGATCGGGGCTGGTGTCGCATT                                                                       >  SRR4302537.3872880/1‑94 (MQ=60)

                        GGAAGAACCGGTAAAACGCGATAGCCCACGTCGTGCCTTCCTGATGATTATGTGGGGCATTGT‑CGGGGG‑GG‑CTGATCGGGGCTGGTGTCGCATT                                                                       >  SRR4302537.2357866/1‑94 (MQ=60)

                         GAAGAACCGGTAAAACGCGATAGCCCACGTCGTGCCTTCCTGATGATTATGTGGGGCATTGT‑CGGGGG‑GG‑CTGATCGGGGCTGGTGTCGCATTA                                                                      >  SRR4302537.5842483/1‑94 (MQ=60)

                          AAGAACCGGTAAAACGCGATAGCCCACGTCGTGCCTTCCTGATGATTATGTGGGGCATTGT‑CGGGGG‑GG‑CTGATCGGGGCTGGTGTCGCATTAA                                                                     >  SRR4302537.186633/1‑94 (MQ=60)

                           AGAACCGGTAAAACGCGATAGCCCACGTCGTGCCTTCCTGATGATTATGTGGGGCATTGT‑CGGGGG‑GG‑CTGATCGGGGCTGGTGTCGCATTAAC                                                                    <  SRR4302537.1946195/94‑1 (MQ=60)

                           AGAACCGGTAAAACGCGATAGACCACGTCGTGCCTTCCTGATGATTATGTGGGGCATTGT‑CGGGGG‑GG‑CTGATCGGGGCTGGTGTCGCATTAAC                                                                    >  SRR4302537.4955864/1‑94 (MQ=60)

                            GAACCGGTAAAACGCGATAGCCCACGTCGTGCCTTCCTGATGATTATGTGGGGCATTGT‑CGGGGG‑GG‑CTGATCGGGGCTGGTGTCGCATTAACC                                                                   >  SRR4302537.2723138/1‑94 (MQ=60)

                             AACCGGTAAAACGCGATAGCCCACGTCGTGCCTTCCTGATGATTATGTGGGGCATTGT‑CGGGGG‑GG‑CTGATCGGGGCTGGTGTCGCGTTAACCC                                                                  >  SRR4302537.3632696/1‑94 (MQ=60)

                                CGGTAAAACGCGATAGCCCACGTCGTGCCTTCCTGATGATTATGTGGGGCATTGT‑CGGGGG‑GG‑CTGATCGGGGCTGGTGTCGCATTAACCCGCC                                                               >  SRR4302537.1023596/1‑94 (MQ=60)

                                CGGTAAAACGCGATAGCCCACGTCGTGCCTTCCTGATGATTATGTGGGGCATTGT‑CGGGGG‑GG‑CTGATCGGGGCTGGTGTCGCATTAACCCGCC                                                               >  SRR4302537.4676046/1‑94 (MQ=60)

                                  GTAAAACGCGATAGCCCACGTCGTGCCTTCCTGATGATTATGTGGGGCATTGT‑CGGGGG‑GG‑CTGATCGGGGCTGGTGTCGCATTAACCCGCCGT                                                             >  SRR4302537.4914988/1‑94 (MQ=60)

                                   TAAAACGCGATAGCCCACGTCGTGCCTTCCTGATGATTATGTGGGGCATTGT‑CGGGGG‑GG‑CTGATCGGGGCTGGTGTCGCATTAACCCGCCGTT                                                            >  SRR4302537.3848607/1‑94 (MQ=60)

                                    AAAACGCGATAGCCCACGTCGTGCCTTCCTGATGATTATGTGGGGCATTGT‑CGGGGG‑GG‑CTGATCGGGGCTGGTGTCGCATTAACCCGCCGTTG                                                           <  SRR4302537.2312606/94‑1 (MQ=60)

                                     AAACGCGATAGCCCACGTCGTGCCTTCCTGATGATTATGTGGGGCATTGT‑CGGGGG‑GG‑CTGATCGGGGCTGGTGTCGCATTAACCCGCCGTTGC                                                          >  SRR4302537.3532571/1‑94 (MQ=60)

                                     AAACGCGATAGCCCACGTCGTGCCTTCCTGATGATTATGTGGGGCATTGT‑CGGGGG‑GG‑CTGATCGGGGCTGGCGTCGCATTAACCCGCCGTTGC                                                          >  SRR4302537.6029656/1‑94 (MQ=60)

                                       ACGCGATAGCCCACGTCGTGCCTTCCTGATGATTATGTGTGGCATTGT‑CGGGGG‑GG‑CTGATCGGGTCTGGTGTCGCATTAACCCGCCGTTGCTC                                                        <  SRR4302537.2294451/94‑1 (MQ=60)

                                        CGCGATAGCCCACGTCGTGCCTTCCTGATGATTATGTGGGGCATTGT‑CGGGGG‑GG‑CTGCTCGGGGCTGGTGTCGCATTAACCCGCCGTTGCTCG                                                       >  SRR4302537.6495128/1‑94 (MQ=60)

                                        CGCGATAGCCCACGTCGTGCCTTCCTGATGATTATGTGGGGCATTGT‑CGGGGG‑GG‑CTGATCGGGGCTGGTGTCGCATTAACCCGCCGTTGCTCG                                                       <  SRR4302537.4811401/94‑1 (MQ=60)

                                        CGCAATAGGCCACGTCGTGCCTTCCTGATGATTATGTGGGGCATTGTGG‑GGGG‑GG‑CTGATCGGGGCTGGTGTCGCATTAACCCGCCGTTGCTCG                                                       <  SRR4302537.5251009/94‑1 (MQ=60)

                                          CGATAGCCCACGTCGTGCCTTCCTGATGATTATGTGGGGCATTGT‑CGGGGG‑GG‑CTGATCGGTGCTGGTGTCGCATTAACCCGCCGTTGCTCGAA                                                     >  SRR4302537.2468850/1‑94 (MQ=60)

                                           GATAGCCCACGTCGTGCCTTCCTGATGATTATGTGGGGCATTGT‑CGGGGG‑GG‑CTGATCGGGGCTGGTGTCGCATTAACCCGCCGTTGCTCGAAA                                                    >  SRR4302537.3228292/1‑94 (MQ=60)

                                            ATAGCCCACGTCGTGCCTTCTTGATGATTATGTGGGGCATTGT‑CGGGGG‑GG‑CTGATCGGGGCTGGTGTCGCATTAACCCGCCGTTGCTCGAAAT                                                   <  SRR4302537.390599/94‑1 (MQ=60)

                                            ATAGCCCACGTCGTGCCTTCCTGATGATTATGTGGGGCATTGT‑CGGGGG‑GG‑CTGATCGGGGCTGGTGTCGCATTAACCCGCCGTTGCTCGAAAT                                                   >  SRR4302537.1567926/1‑94 (MQ=60)

                                            ATAGCCCACGTCGTGCCTTCCTGATGATTATGTGGGGCATTGT‑CGGGGG‑GG‑CTGATCGGGGCTGGTGTCGCATTAACCCGCCGTTGCTCGAAAT                                                   >  SRR4302537.4922529/1‑94 (MQ=60)

                                             TAGCCCACGTCGTGCCTTCCTGATGATTATGTGGGGCATTGT‑CGGGGG‑GG‑CTGATCGGGGCTGGTGTCGCATTAACCCGCCGTTGCTCGAAATA                                                  >  SRR4302537.3536407/1‑94 (MQ=60)

                                             TAGCCCACGTCGTGCCTTCCTGATGATTATGTGGGGCATGGT‑CGGGGG‑GG‑CTGATCGGGGCTGGTTTCGCATTAACCCGCCGTTGCTCGAAATA                                                  <  SRR4302537.2393722/94‑1 (MQ=60)

                                              AGCCCACGTCGTGCCTTCCTGATGATTATGTGGGGCATTGT‑CGGGGG‑GG‑CTGATCGGGGCTGGTGTCGCATTAACCCGCCGTTGCTCGAAATAG                                                 <  SRR4302537.5242175/94‑1 (MQ=60)

                                              AGCCCACGTCGTGCCTTCCTGATGATTATGTGGGGCATTGT‑CGGGGG‑GG‑CTGATCGGGGCTGGTGTCGCATTAACCCGCCGTTGCTCGAAATAG                                                 <  SRR4302537.4709430/94‑1 (MQ=60)

                                               GCCCACGTCGTGCCTTCCTGATGATTATGTGGGGCATTGT‑CGGGGG‑GG‑CTGATCGGGGCTGGTGTCGCATTAACCCGCCGTTGCTCGAAATAGC                                                >  SRR4302537.2233142/1‑94 (MQ=60)

                                                CCCACGTCGTGCCTTCCTGATGATTATGTGGGGCATTGT‑CGGGGG‑GG‑CTGATCGGGGCTGGTGTCGCATTAACCCGCCGTTGCTCGAAATAGCA                                               >  SRR4302537.5130982/1‑94 (MQ=60)

                                                CCCACGTCGTGCCTTCCTGATGATTATGTGGGGCATTGT‑CGGGGG‑GG‑CTGATCGGGGCTGGTGTCGCATTAACCCGCCGTTGCTCGAAATAGCA                                               <  SRR4302537.309370/94‑1 (MQ=60)

                                                CCCACGTCGTGCCTTCCTGATGATTATGTGGGGCATTGT‑CGGGGG‑GG‑CTGATCGGGGCTGGTGTCGCATTAACCCGCCGTTGCTCGAAATAGCA                                               <  SRR4302537.3759960/94‑1 (MQ=60)

                                                 CCGCGTCGTGCCTTCCTGATGTTTAGGTGGGGCATTGT‑CGGGGG‑GG‑CTGATCGGGGCTGGTGTCGCATTAACCCGCCGTTGCTCGAAATAGCAA                                              <  SRR4302537.2288400/94‑1 (MQ=60)

                                                  CACGTCGTGCCTTCCTGATGATTATGTGGGGCATTGT‑CGGGGG‑GG‑CTGATCGGGGCTGGTGTCGCATTAACCCGCCGTTGCTCGAAATAGCAAC                                             >  SRR4302537.4563111/1‑94 (MQ=60)

                                                  CACGTCGTGCCTTCCTGATGATTATGTGGGGCATTGT‑CGGGGG‑GG‑CTGATCGGGGCTGGTGTCGCATTAACCCGCCGTTGCTCGAAATAGCAAC                                             >  SRR4302537.4314408/1‑94 (MQ=60)

                                                  CACGTCGTGCCTTCCTGATGATTATGTGGGGCATTGT‑CGGGGG‑GG‑CTGATCGGGGCTGGTGTCGCATTAACCCGCCGTTGCTCGAAATAGCAAC                                             <  SRR4302537.274175/94‑1 (MQ=60)

                                                   cgggCGTGCTTTCCTGATTATTATGTGGGCCATTGT‑CGGGGG‑GG‑CTGAGCGGGGCTGGTGTCGCATTACCCCGCCGTTGCTCGAAATAGCAACA                                            <  SRR4302537.3360403/90‑1 (MQ=60)

                                                   CCGTCGTGCCTTCCTGATGATTATGTGGGGCATTGT‑CGGGGG‑GG‑CTGATCGGGGCTGGTGTCGCATTAACCCGCCGTTGCTCGAAATAGCAACA                                            <  SRR4302537.3681926/94‑1 (MQ=60)

                                                   CCGTCGTGCCTTCCTGATGATTATGTGGGCCATTGT‑CGGGGG‑GG‑CTGATCGGGGCTGGTGTCGCATTAACCCGCCGTTGCTCGAAATAGCAACA                                            <  SRR4302537.2547161/94‑1 (MQ=60)

                                                   ACGTCGTGCCTTCCTGATGATTATGTGGGGCATTGT‑CGGGGG‑GG‑CTGATCGGGGCTGGTGTCGCATTAACCCGCCGTTGCTCGAAATAGCAACA                                            >  SRR4302537.1241490/1‑94 (MQ=60)

                                                   ACGTCGTGCCTTCCTGATGATTATGTGGGGCATTGT‑CGGGGG‑GG‑CTGATCGGGGCTGGTGTCGCATTAACCCGCCGTTGCTCGAAATAGCAACA                                            <  SRR4302537.528925/94‑1 (MQ=60)

                                                     GTCGTTCCTGCCTGATGATTATGTGGGGCATTGT‑CGGGGG‑GG‑CTGATCGGGGCTGGTGTCGCATTAACCCGCCGTTGCTCGAAATAGCAACACT                                          <  SRR4302537.1806503/94‑1 (MQ=60)

                                                     GTCGTGCCTTCCTGATGATTATGTGGGGCATTGT‑CGGGGG‑GG‑CTGATCGGGGCTGGTGTCGCATTAACCCGCCGTTGCTCGAAATAGCAACACT                                          >  SRR4302537.5709311/1‑94 (MQ=60)

                                                     GTCGTGCCTTCCTGATGATTATGTGGGGCATTGT‑CGGGGG‑GG‑CTGATCGGGGCTGGTGTCGCATTAACCCGCCGTTGCTCGAAATAGCAACACT                                          >  SRR4302537.2123398/1‑94 (MQ=60)

                                                     GTCGTGCCTTCCTGATGATTATGTGGGGCATTGT‑CGGGGG‑GG‑CTGATCGGGGCTGGTGTCGCATTAACCCGCCGTTGCTCGAAATAGCAAAACT                                          >  SRR4302537.785142/1‑94 (MQ=60)

                                                     GTCGTGCCTTCCTGATGATTATGTGGGGCATTGT‑CGGGGG‑GG‑CTGATCGGGGCTGGTGTCGCATTAACCCGACGGTGCTCGAAATAGCAACACT                                          >  SRR4302537.5125003/1‑94 (MQ=60)

                                                        GTGCCTTCCTGATGATTATGTGGGGCATTGT‑CGGGGG‑GG‑CTGATCGGGGCTGGTGTCGCATTAACCCGCCGTTGCTCGAAATAGCAACACTGCT                                       >  SRR4302537.5113529/1‑94 (MQ=60)

                                                        GTGCCTTCCTGATGATTATGTGGGGCATTGT‑CGGGGG‑GG‑CTGATCGGGGCTGGTGTCGCATTAACCCGCCGTTGCTCGAAATAGCAACACTGCT                                       <  SRR4302537.5255441/94‑1 (MQ=60)

                                                         TGCCTTCCTGATGATTATGTGGGGCATTGT‑CGGGGG‑GG‑CTGATCGGGGCTGGTGTCGCATTAACCCGCCGTTGCTCGAAATAGCAACACTGCTG                                      <  SRR4302537.4800482/94‑1 (MQ=60)

                                                          GCCTTCCTGATGATTATGTGGGGCATTGT‑CGGGGG‑GG‑CTGATCGGGGCTGGTGTCGCATTAACCCGCCGTTGCTCGAAATAGCAACACTGCTGC                                     >  SRR4302537.4857447/1‑94 (MQ=60)

                                                          GCCTTCCTGATGATTATGTGGGGCATTGT‑CGGGGG‑GG‑CTGATCGGGGCTGGTGTCGCATTAACCCGCCGTTGCTCGAAATAGCAACACTGCTGC                                     >  SRR4302537.1592933/1‑94 (MQ=60)

                                                          GCCTTCCTGATGATTATGTGGGGCATTGT‑CGGGGG‑GG‑CTGATCGGGGCTGGTGTCGCATTAACCCGCCGTTGCTCGAAATAGCAACACTGCTGC                                     <  SRR4302537.1164899/94‑1 (MQ=60)

                                                          GCCTTCCTGATGATTATGTGGGGCATTGT‑CGGGGG‑GG‑CTGATCGGGGCTGGTGTCGCATTAACCCGCCGTTGCTCGAAATAGCAACACTGCTGC                                     >  SRR4302537.234867/1‑94 (MQ=60)

                                                            CTTCCTGATGATTATGTGGGGCATTGT‑CGGGGG‑GG‑CTGATCGGGGCTGGTGTCGCATTAACCCGCCGTTGCTCGAAATAGCAACACTGCTGCGG                                   >  SRR4302537.5893372/1‑94 (MQ=60)

                                                            CTTCCTGATGATTATGTGGGGCATTGT‑CGGGGG‑GG‑CTGATCGGGGCTGGTGTCGCATTAACCCGCCGTTGCTCGAAATAGCAACACTGCTGCAG                                   >  SRR4302537.2779193/1‑94 (MQ=60)

                                                             TTCCTGATGATTATGTGGGGCATTGT‑CGGGGG‑GG‑CTGATCGGGGCTGGTGTCGCATTAACCCGCCGTTGCTCGAAATAGCAACACTGCTGCGGT                                  >  SRR4302537.4767058/1‑94 (MQ=60)

                                                             TTCCTGATGATTATGTGGGGCATTGT‑CGGGGG‑GG‑CTGATCGGGGCTGGTGTCGCATTAACCCGCCGTTGCTCGAAATAGCAACACTGCTGCGGT                                  >  SRR4302537.5466416/1‑94 (MQ=60)

                                                              TCCTGATGATTATGTGGGGCATTGT‑CGGGGG‑GG‑CTGATCGGGGCTGTTGTCGCATTAACCCGCCGTTGCTCGAAATAGCAACACTGCTGCGGTG                                 <  SRR4302537.5585390/94‑1 (MQ=60)

                                                              TCCTGATGATTATGTGGGGCATTGT‑CGGGGG‑GG‑CTGATCGGGGCTGGTGTCGCATTAACCCGCCGTTGCTCGAAATAGCAACACTGCTGCGGTG                                 <  SRR4302537.6062518/94‑1 (MQ=60)

                                                               CCTGATGATTATGTGGGGCATTGT‑CGGGGG‑GG‑CTGATCGGGGCTGGTGTCGCATTAACCCGCCGTTGCTCGAAATAGCAACACTGCTGCGGTGA                                >  SRR4302537.4590247/1‑94 (MQ=60)

                                                               CCTGATGATTATGTGGGGCATTGT‑CGGGGG‑GG‑CTGATCGGGGCTGGTGTCGCATTAACCCGCCGTTGCTCGAAATAGCAACACTGCTGCGGTGA                                >  SRR4302537.2079517/1‑94 (MQ=60)

                                                               CCTGATGATTATGTGGGGCATTGT‑CGGGGG‑GG‑CTGATCGGGGCTGGTGTCGCATTAACCCGCCGTTGCTCGAAATAGCAACACTGCTGCGGTGA                                >  SRR4302537.4957824/1‑94 (MQ=60)

                                                                CTGATGATTATGTGGGGCATTGT‑CGGGGG‑GG‑CTGATCGGGGCTGGTGTCGCATTAACCCGCCGTTGCTCGAAATAGCAACACTGCTGCGGTGAG                               >  SRR4302537.4995760/1‑94 (MQ=60)

                                                                 TGATGATTATGTGGGGCATTGT‑CGGGGG‑GG‑CTGATCGGGGCTGGTGTCGCATTAACCCGCCGTTGCTCGAAATAGCAACACTGCTGCGGTGAGC                              >  SRR4302537.3513287/1‑94 (MQ=60)

                                                                  GATGATTATGTGGGGCATTGT‑CGGGGG‑GG‑CGGATGGGGGCTGGTGTCGCATTACCCCGCCGTTGCTCGAAATAGCAACACTGCTGCGGTGAGCG                             <  SRR4302537.5077814/94‑1 (MQ=60)

                                                                      ATTATGTGGGGCATTGT‑CGGGGG‑GG‑CTGATCGGGGCTGGTGTCGCATTAACCCGCCGTTGCTCGAAATAGCAACACTGCTGCGGTGAGCGCAAA                         <  SRR4302537.3511121/94‑1 (MQ=60)

                                                                       TTATGTGGGGCATTGT‑CGGGGG‑GG‑CTGATCGGGGCTGGTGTCGCATTAACCCGCCGTTGCTCGAAATAGCAACACTGCTGCGGTGAGCGCAAAG                        <  SRR4302537.2375581/94‑1 (MQ=60)

                                                                       TTATGTGGGGCATTGT‑CGGGGG‑GG‑CTGATCGGGGCTGGTGTCGCATTAACCCGCCGTTGCTCGAAATAGCAACACTGCTGCGGTGAGCGCAAAG                        >  SRR4302537.4652979/1‑94 (MQ=60)

                                                                       TTATGTGGGGCATTGT‑CGGGGG‑GG‑CTGATCGGGGCTGGTGTCGCATTAACCCGCCGTTGCTCGAAATAGCAACACTGCTGCGGTGAGCGCAAAG                        <  SRR4302537.1423304/94‑1 (MQ=60)

                                                                         ATGTGGGGCATTGT‑CGGGGG‑GG‑CTGATCGGGGCGGGTGTCGCATTAACCCGCCGTTGCTCGAAATAGCAACACTGCTGCGGTGAGCGCAAAGGC                      <  SRR4302537.2045597/94‑1 (MQ=60)

                                                                         ATGTGGGGCATGGT‑CGGGGG‑GG‑CTGATCGGGGCTGGTGTCGCATTAACCCGCCGTTGCTCGAAATAGCAACACTGCTGCGGTGAGCGCAAAGGC                      <  SRR4302537.5117862/94‑1 (MQ=60)

                                                                          TGTGGGGCATGGG‑CGGGGG‑GG‑CTGATCGGGGCTGGTGTCGCATTAACCCGCCGTTGCTCGAAATAGCAACACTGCTGCGGTGAGCGCAAAGGCG                     <  SRR4302537.5632416/94‑1 (MQ=60)

                                                                           GTGGGGCATTGT‑CGGGGG‑GG‑CTGATCGGGGCTGGTGTCGCATTAACCCGCCGTTGCTCGAAATAGCAACACTGCTGCGGTGAGCGCAAAGGCGC                    <  SRR4302537.3723978/94‑1 (MQ=60)

                                                                            TGGGGCATTGT‑CGGGGG‑GG‑CTGATCGGGGCTGGTGTCGCATTAACCCGCCGTTGCTCGAAATAGCAACACTGCTGCGGTGAGCGCAAAGGCGCT                   >  SRR4302537.2116607/1‑94 (MQ=60)

                                                                              GGGCATTGT‑CGGGGG‑GG‑CTGATCGGGGCTGGTGTCGCATTAACCCGCCGTTGCTCGAAATAGCAACACTGCTGCGGTGAGCGCAAAGGCGCTCG                 <  SRR4302537.1485025/94‑1 (MQ=60)

                                                                               GGCATTGT‑CGGGGG‑GG‑CTGATCTGGGCTGGTGTCGCATTAACCCGCCGTTGCTCGAAATAGCAACACTGCTGCGGTGAGCGCAAAGGCGCTCGC                >  SRR4302537.6184299/1‑94 (MQ=60)

                                                                               GGCATTGT‑CGGGGG‑GG‑CTGATCGGGGCTGGTGTCGCATTAACCCGCCGTTGCTCGAAATAGCAACACTGCTGCGGTGAGCGCAAAGGCGCTCGC                >  SRR4302537.3449928/1‑94 (MQ=60)

                                                                                GCATTGT‑CGGGGG‑GG‑CTGATCGGGGCTGGTGTCGCATTACCCCGCCGTTGCTCGAAATAGCAACACTGCTGCGGTGAGCGCAAAGGCGCTCGCC               <  SRR4302537.3880128/94‑1 (MQ=60)

                                                                                GCATTGT‑CGGGGG‑GG‑CTGATCGGGGCTGGTGTCGCATTAACCCGCCGTTGCTCGAAATAGCAACACTGCTGCGGTGAGCGCAAAGGCGCTCGCC               <  SRR4302537.2616498/94‑1 (MQ=60)

                                                                                GCATTGT‑CGGGGG‑GG‑CTGATCGGGGCTGGTGTCGCATTAACCCGCCGTTGCTCGAAATAGCAAAACTGCTGCGGTGAGCGCAAAGGCGCTCGCC               >  SRR4302537.1810413/1‑94 (MQ=60)

                                                                                  ATTGT‑CGGGGG‑GG‑CTGATCGGGGCTGGTGTCGCATTAACCCGCCGTTGCTCGAAATAGCAACACTGCTGCGGTGAGCGCAAAGGCGCTCGCCGC             >  SRR4302537.91726/1‑94 (MQ=60)

                                                                                   TTGT‑CGGGGG‑GG‑CTGATCGGGGCTGGTGTCGCATTAACCCGCCGTTGCTCGAAATAGCAACACTGCTGCGGTGAGCGCAAAGGCGCTCGCCGCT            <  SRR4302537.4387233/94‑1 (MQ=60)

                                                                                    TGT‑CGGGGG‑GG‑CTGATCGGGGCTGGTGTCGCATTAACCCGCCGTTGCTCGAAATAGCAACACTGCTGCGGTGAGCGCAAAGGCGCTCGCCGCTT           >  SRR4302537.354678/1‑94 (MQ=60)

                                                                                    TGT‑CGGGGG‑GG‑CTGATCGGGGCTGGTGTCGCATTAACCCGCCGTTGCTCGAAATAGCAACACTGCTGCGGTGAGCGCAAAGGCGCTCGCCGCTT           <  SRR4302537.1718426/94‑1 (MQ=60)

                                                                                    TGT‑CGGGGG‑GG‑CTGATCGGGGCTGGTGTCGCATTAAACCGCCGTTGCTCGAAATAGCAACACTGCTGCGGTGAGCGCAAAGGCGCTCGCCGCTT           <  SRR4302537.2750741/94‑1 (MQ=60)

                                                                                        cgGGGG‑GG‑CTGATCGGGGCTGGTGTCGCATTAACCCGCCGTTGCTCGAAATAGCAACACTGCTGCGGTGAGCGCAAAGGCGCTCGCCGCTTATT        >  SRR4302537.4070479/3‑94 (MQ=60)

                                                                                        cgGGGG‑GG‑CTGATCGGGGCTGGTGTCGCATTAACCCGCCGTTGCTCGAAATAGCAACACTGCTGCGGTGAGCGCAAAGGCGCTCGCCGCTTATT        >  SRR4302537.839596/3‑94 (MQ=60)

                                                                                        G‑GGGG‑GG‑CTGATCGGGGCTGGTGTCGCATTAACCCGCCGTTGCTCGAAATAGCAACACTGCTGCGGTGAGCGCAAAGGCGCTCGCCGCTTATTC       >  SRR4302537.5033479/1‑94 (MQ=60)

                                                                                         gtGGG‑GG‑CTGATCGGGCCTGGTGTCTCATTAGCCCGCCGTTGCTCGAAATAGCAACACTGCTGCGGTGAGCGCAAAGGCGCTCGCCGCTTATTC       <  SRR4302537.168261/92‑1 (MQ=60)

                                                                                          GGGG‑GG‑CTGATCGGGGCTGGTGTCGCATTAACCCGCCGTTGCTCGAAATAGCAACACTGCTGCGGTGAGCGCAAAGGCGCTCGCCGCTTATTCG      <  SRR4302537.5436678/94‑1 (MQ=60)

                                                                                          GGGG‑GG‑CTGATCGGGGCTGGTGTCGCATTAACCCGCCGTTGCTCGAAATAGCAACACTGCTGCGGTGAGCGCAAAGGCGCTCGCCGCTTATTCG      >  SRR4302537.6339834/1‑94 (MQ=60)

                                                                                             G‑TG‑CTGATCGGGGCGGGTGTCGCATTAACCCGCCGTTGCTCGAAATAGCAACACTGCTGCGGTGAGCGCAAAGGCGCTCGCCGCTTATTCGAAG   <  SRR4302537.1969574/94‑1 (MQ=60)

                                                                                             G‑GG‑TTGATGGGGGCTGGTGTCGCATTAACCCGCCGTTGCTCGAAATAGCAACACTGCTGCGGTGAGCGCAAAGGCGCTCGCCGCTTATTCGAAG   <  SRR4302537.1818902/94‑1 (MQ=60)

                                                                                             G‑GG‑CTGATCGGGGCTGGTGTCGCATTAACCCGCCGTTGCTCGAAATAGCAACACTGCTGCGGTGAGCGCAAAGGCGCTCGCCGCTTATTCGAAG   <  SRR4302537.3189074/94‑1 (MQ=60)

                                                                                             G‑GG‑CTGATCGGGGCTGGTGTCGCATTAACCCGCCGTTGCTCGAAATAGCAACACTGCTGCGGTGAGCGCAAAGGCGCTCGCCGCTTATTCGAAG   <  SRR4302537.805379/94‑1 (MQ=60)

                                                                                             G‑GG‑CTGATCGGGGCTGGTGTCGCATTAACCCGCCGTTGATCGAAATAGCAACACTGCTGCGGTGAGAGCAAAAGCGCTCGCCGCTTATTCGAAA   >  SRR4302537.4268066/1‑94 (MQ=60)

                                                                                               GG‑CTGATCGGGGCTGGTGTCGCATTACCCCGCCGTTGCTCGAAATAGCAACACTGCTGCGGTGAGCGCAAAGGCGCTCGCCGCTTATTCGAAGA  <  SRR4302537.2670894/94‑1 (MQ=60)

                                                                                               GG‑CTGATCGGGGCTGGTGTCGCATTAACCCGCCGTTGCTCGAAATAGCAACACTGCTGCGGTGAGCGCAAAGGCGCTCGCCGCTTATTCGAAGA  >  SRR4302537.2197018/1‑94 (MQ=60)

GACCTATCGCTATTTGCGTACGCCGGAAGAACCGGTAAAACGCGATAGCCCACGTCGTGCCTTCCTGATGATTATGTGGGGCATTGT‑C‑GGGG‑GG‑CTGATCGGGGCTGGTGTCGCATTAACCCGCCGTTGCTCGAAATAGCAACACTGCTGCGGTGAGCGCAAAGGCGCTCGCCGCTTATTCGAAGA  >  NC_000913/3967554‑3967739

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 0 ≤` `ATCG/ATCG` `< 2 ≤` `ATCG/ATCG` `< 9 ≤` `ATCG/ATCG` `< 31 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`