Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I2 R1 882 254.4 12957588 98.0% 12698436 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,969,103 2 bp→GC coding (232‑233/1263 nt) wecC → UDP‑N‑acetyl‑D‑mannosaminuronic acid dehydrogenase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,969,1030CG100.0% 725.0 / NA 211R78A (CGC→GCC) wecCUDP‑N‑acetyl‑D‑mannosaminuronic acid dehydrogenase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base G (85/126);  total (85/126)
*NC_0009133,969,1040GC100.0% 704.7 / NA 206R78A (CGC→GCC) wecCUDP‑N‑acetyl‑D‑mannosaminuronic acid dehydrogenase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base C (81/125);  total (81/125)

Only 100 of 212 total aligned reads displayed.

ATCGTCGAACCTGATTTGGCGAGTGTAGTAAAAACTGCCGTAGAAGGCGGTTTTTTACGAGCGAGCACGACGCCAGTTGAAGCGGATCGCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATGAGCCAGATATGACCTACGTTGAATCGGCTGCTCGCTCCATTGCG  >  NC_000913/3969016‑3969189
                                                                                       ||                                                                                     
    tcgAACCTGATTTGGCGAGTGTAGTAAAAACTGCCGTAGAAGGCGGTTTTTTACGAGCGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGa                                                                               >  2:224350/1‑93 (MQ=255)
    tcgAACCGGATTGGGCGAGTGTAGTAAAAACTGCCGTAGAAGGCGGTTTTTTACGAGCGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGa                                                                               <  2:5970562/93‑1 (MQ=255)
     cgAACCTGATTTGGCGAGTGTAGTAAAAACTGCCGTAGAAGGCGGTTTTTTACGAGCGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGAt                                                                              <  2:2774875/93‑1 (MQ=255)
       aaCCTGATTTGGCGAGTGTAGTAAAAACTGCCGTAGAAGGCGGTTTTTTACGAGCGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTg                                                                            >  1:1092888/1‑93 (MQ=255)
        aCCTGATTTGGCGAGTGTAGTAAAAACTGCCGTAGAAGGCGGTTTTTTACGAGCGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGc                                                                           <  2:5189964/93‑1 (MQ=255)
        aCCTGATTTGGCGAGTGTAGTAAAAACTGCCGTAGAAGGCGGTTTTTTACGAGCGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGc                                                                           >  2:1381284/1‑93 (MQ=255)
        aCCTGATTTGGCGAGTGTAGTAAAAACTGCCGTAGAAGGCGGTTTTTTACGAGCGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGc                                                                           <  1:3821953/93‑1 (MQ=255)
        aCCTGATTTGGCGAGTGTAGTAAAAACTGCCGTAGAAGGCGGTTTTTTACGAGCGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGc                                                                           >  2:1851852/1‑93 (MQ=255)
         ccTGTTTTGGCGAGTGTAGTAAAAACTGCCGTAGAAGGCGGTTTTTTACGAGCGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCt                                                                          <  2:4674709/93‑1 (MQ=255)
         ccTGATTTGGCGAGTGTAGTAAAAACTGCCGTAGAAGGCGGTTTTTTACGAGCGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCt                                                                          <  2:1794556/93‑1 (MQ=255)
           tGATTTGGCGAGTGTAGTAAAAACTGCCGTAGAAGGCGGTTTTTTACGAGCGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGt                                                                        >  2:5587859/1‑93 (MQ=255)
           tGATTTGGCGAGTGTAGTAAAAACTGCCGTAGAAGGCGGTTTTTTACGAGCGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGt                                                                        <  1:3211861/93‑1 (MQ=255)
           tGATTTGGCGAGTGTAGTAAAAACTGCCGTAGAAGGCGGTTTTTTACGAGCGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGt                                                                        >  2:584702/1‑93 (MQ=255)
             aTTTGGCGAGTGTAGTAAAAACTGCCGTAGAAGGCGGTTTTTTACGAGCGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTAc                                                                      >  2:2422206/1‑93 (MQ=255)
             aTTTGGCGAGTGTAGTAAAAACTGCCGTAGAAGGCGGTTTTTTACGAGCGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTAc                                                                      <  1:3754271/93‑1 (MQ=255)
             aTTGGGCGAGTGTAGT‑AAAGCTGCCGTAGAAGTCGGTTTTTTACGAGCGATCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTAcc                                                                     <  1:3096987/93‑1 (MQ=255)
                tGGCGAGTGTAGTAAAAACTGCCGTAGAAGGCGGTTTTTTACGAGCGAGCACGACGCCAGTTTAAGCGGATGCCTGGCTGATTGCTGTACCCa                                                                   >  1:5121510/1‑93 (MQ=255)
                tGGCGAGTGTAGTAAAAACTGCCGTAGAAGGCGGTTTTTTACGAGCGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCa                                                                   >  2:1294842/1‑93 (MQ=255)
                tGGCGAGTGTAGTAAAAACTGCCGTAGAAGGCGGTTTTTTACGAGCGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCa                                                                   <  2:3017945/93‑1 (MQ=255)
                   cGAGTGTAGTAAAAACTGCCGTAGAAGGCGGTTTTTTACGAGCGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGc                                                                >  2:5125266/1‑93 (MQ=255)
                       tgtAGTAAAAACTGCCGTAGAAGGCGGTTTTTTACGAGCGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGtt                                                            <  1:3144812/93‑1 (MQ=255)
                         tagtaAAAACTGCCGTAGAAGGCGGTTTTTTACGAGCGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTa                                                          >  2:5685399/1‑93 (MQ=255)
                         tagtaAAAACTGCCGTAGAAGGCGGTTTTTTACGAGCGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTa                                                          <  2:1581378/93‑1 (MQ=255)
                         tagtaAAAACTGCCGTAGAAGGCGGTTTTTTACGAGCGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTa                                                          >  2:708701/1‑93 (MQ=255)
                         aagtaAAAACTGCCGTAGAAGGCGGTTTTTTACGAGCTAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTa                                                          <  1:2605630/92‑1 (MQ=255)
                          agtaAAAACTGCCGTAGAAGGCGGTTTTTTACGAGCGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTaa                                                         <  1:2029021/93‑1 (MQ=255)
                           gtaAAAACTGCCGTAGAAGGCGGTTTTTTACGAGCGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAg                                                        <  1:2790977/93‑1 (MQ=255)
                           gtaAAAACTGCCGTAGAAGGCGGTTTTTTACGAGCGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAg                                                        >  1:922636/1‑93 (MQ=255)
                            taAAAACTGCCGTAGAAGGCGGTTTTTTACGAGCGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAgg                                                       <  1:3190172/93‑1 (MQ=255)
                            taAAAACTGCCGTAGAAGGCGGTTTTTTACGAGCGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAgg                                                       >  1:25295/1‑93 (MQ=255)
                              aaaaCTGCCGTAGAAGGCGTTTTTTTACGAGCGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGc                                                     <  1:5789167/93‑1 (MQ=255)
                              aaaaCTGCCGTAGAAGGCGGTTTTTTACGAGCGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGc                                                     <  2:3976845/93‑1 (MQ=255)
                               atccTGCGGGAGAAGGCGGTTTTTTACGAGAGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCg                                                    <  1:3496261/90‑1 (MQ=255)
                               aaaCTGCCGTAGAAGGCGGTTTTTTACGAGCGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCg                                                    >  1:4295110/1‑93 (MQ=255)
                                 aCTGCCGTAGAAGGCGGTTTTTTACGAGCGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGAt                                                  <  1:1490879/93‑1 (MQ=255)
                                 aCTGCCGTAGAAGGCGGTTTTTTACGAGCGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGAt                                                  >  2:3859605/1‑93 (MQ=255)
                                  cTGCCGTAGAAGGCGGTTTTTTACGAGCGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATc                                                 >  1:264422/1‑93 (MQ=255)
                                   tGCCGTAGAAGGCGGTTTTTTACGAGCGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCa                                                <  2:2149055/93‑1 (MQ=255)
                                   tGCCGTAGAAGGCGGTTTTTTACGAGCGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCa                                                >  2:808876/1‑93 (MQ=255)
                                    gCCGTAGAAGGCGGTTTTTTACGAGCGAACACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCAt                                               <  1:2281167/93‑1 (MQ=255)
                                     ccGTATAAGGCGTTTTTTAACGAGCGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATg                                              <  2:5750123/93‑1 (MQ=255)
                                     ccGTAGAAGGCGGTTTTTTACGAGCGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATg                                              <  2:5259821/93‑1 (MQ=255)
                                      cGTAGAAGGCGGTTTTTTACGAGCGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATGa                                             <  2:2717712/93‑1 (MQ=255)
                                      cGTAGAAGGCGGTTTTTTACGAGCGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATGa                                             <  2:6015309/93‑1 (MQ=255)
                                       gTAGAAGGCGGTTTTTTACGAGCGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATGAg                                            >  2:2552223/1‑93 (MQ=255)
                                        tAGAAGGCGGTTTTTTACGAGCGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATGAGc                                           <  2:401900/93‑1 (MQ=255)
                                        tAGAAGGCGGTTTTTTACGAGCGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATGAGc                                           >  1:71223/1‑93 (MQ=255)
                                         aGAAGGCGGTTTTTTACGAGCGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTAGCCACGCCGTTTAAGGGCGATCATGAGcc                                          >  1:2817492/1‑93 (MQ=255)
                                          gAAGGCGGTTTTTTACGAGCGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATGAGCCa                                         >  2:4833173/1‑93 (MQ=255)
                                            aGGCGGTTTTTTACGAGCGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATGAGCCAGa                                       <  1:5658172/93‑1 (MQ=255)
                                            aGGCGGTTTTTTACGAGCGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATGAGCCAGa                                       >  2:6077212/1‑93 (MQ=255)
                                              gCGGTTTTTTACGAGCGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATGAGCCAGata                                     <  1:4225763/93‑1 (MQ=255)
                                              gCGGTTTTTTACGAGCGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATAAGCCAGata                                     <  1:6227005/93‑1 (MQ=255)
                                              gCGGTTTTTGACGAGCGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATGAGCCAGata                                     <  1:1336400/93‑1 (MQ=255)
                                               cGGTTTTTTACGAGCGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATGAGCCAGatat                                    >  1:3446165/1‑93 (MQ=255)
                                                ggTTTTTTACGAGCGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATGAGCCAGATATg                                   <  2:658128/93‑1 (MQ=255)
                                                  ttttttACGAGCGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATGAGCCAGATATGAc                                 <  1:4900111/93‑1 (MQ=255)
                                                   tttttACGAGCGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATGAGCCAGATATGAcc                                <  2:6238198/93‑1 (MQ=255)
                                                   tttttACGAGCGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATGAGCCAGATATGAcc                                >  1:5921399/1‑93 (MQ=255)
                                                     tttACGAGCGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATGAGCCAGATATGACCTa                              <  2:3401217/93‑1 (MQ=255)
                                                      ttACGAGCGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATGAGCCAGATATGACCTAc                             <  2:2665543/93‑1 (MQ=255)
                                                       tACGAGCGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATGAGCCAGATATGACCTACg                            >  2:4586825/1‑93 (MQ=255)
                                                       tACGAGCGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATGAGCCAGATATGACCTACg                            <  1:5337521/93‑1 (MQ=255)
                                                        aCGAGCGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATGAGCCAGATATGACCTACGt                           <  1:846718/93‑1 (MQ=255)
                                                         cgagcgagCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATGAGCCAGATATGACCTACGtt                          <  2:1924451/93‑1 (MQ=255)
                                                         cgagcgagCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATGAGCCAGATATGACCTACGtt                          <  1:5209790/93‑1 (MQ=255)
                                                          gagagaccACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATGAGCCAGATATGACCTACGTTg                         <  1:4425590/93‑1 (MQ=255)
                                                           agcgagcACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATGAGCCAGATATGACCTACGTTGa                        <  1:6304847/93‑1 (MQ=255)
                                                            gcgagcACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATGAGCCAGATATGACCTACGTTGag                       <  2:4175572/93‑2 (MQ=255)
                                                            gcgagcACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATGAGCCAGATATGACCTACGTTGaa                       <  1:3715038/93‑1 (MQ=255)
                                                            gcgagcACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATGAGCCAGATATGACCTACGTTGaa                       <  2:4375767/93‑1 (MQ=255)
                                                            gcgagcACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATGAGCCAGATATGACCTACGTTGaa                       >  1:4382493/1‑93 (MQ=255)
                                                             gaagcACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTCCCCACGCCGTTTAAGGGCGATCATGAGCCAGATATGACCTACGTTGAAt                      <  2:1861011/91‑1 (MQ=255)
                                                             cgagcACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATGAGCCAGATATGACCTACGTTGAAt                      <  1:2733459/93‑1 (MQ=255)
                                                              gagcACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATGAGCCAGATATGACCTACGTTGAATc                     <  1:299888/93‑1 (MQ=255)
                                                              gagcACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATGAGCCAGATATGACCTACGTTGAATc                     <  2:4274194/93‑1 (MQ=255)
                                                              gagcACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATGAGCCAGATATGACCTACGTTGAATc                     <  2:5946886/93‑1 (MQ=255)
                                                              gagcACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATGAGCCAGATATGACCTACGTTGAATc                     <  1:1296383/93‑1 (MQ=255)
                                                               agcACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATGAGCCAGATATGACCTACGTTGAATCg                    >  1:3353042/1‑93 (MQ=255)
                                                                gcACGACGCCAGTTGAAGCGGATGCCTGGTTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATGAGCCAGATATGACCTACGTTGAATCgg                   <  2:5312/93‑1 (MQ=255)
                                                                gcACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATGAGCCAGATATGACCTACGTTGAATCgg                   >  1:3636500/1‑93 (MQ=255)
                                                                gcACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATGAGCCAGATATGACCTACGTTGAATCgg                   <  2:4731132/93‑1 (MQ=255)
                                                                gcACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATGAGCCAGATATGACCTACGTTGAATCgg                   <  1:1428812/93‑1 (MQ=255)
                                                                gcACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATGAGCCAGATATGACCTACGTTGAATCgg                   <  2:1551547/93‑1 (MQ=255)
                                                                 cACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATGAGCCAGATATGACCTACGTTGAATCGgc                  <  2:1499035/93‑1 (MQ=255)
                                                                 cACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATGAGCCAGATATGACCTACGTTGAATCGgc                  <  1:4097244/93‑1 (MQ=255)
                                                                  acgacgCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATGAGCCAGATATGACCTACGTTGAATCGgct                 >  2:616335/1‑93 (MQ=255)
                                                                  acgacgCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATGAGCCAGATATGACCTACGTTGAATCGgct                 <  1:1830933/93‑1 (MQ=255)
                                                                    gacgCCAGTTGGAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAATTGAGATCATGAGCCAGATATGACCTACGTTGAATAGgctgc               >  2:3648260/1‑93 (MQ=255)
                                                                    gacgCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATGAGCCAGATATGACCTACGTTGAATCGgctgc               <  2:3275636/93‑1 (MQ=255)
                                                                          aGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATGAGCCAGATATGACCTACGTTGAATCGGCTgctcgctc         >  1:6047684/1‑93 (MQ=255)
                                                                             tGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATGAGCCAGATATGACCTACGTTGAATCGGCTGCTCGCTCCAt      <  1:1754704/93‑1 (MQ=255)
                                                                               aaGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATGAGCCAGATATGACCTACGTTGAATCGGCTGCTCGCTCCATTg    >  2:5419268/1‑93 (MQ=255)
                                                                               aaGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATGAGCCAGATATGACCTACGTTGAATCGGCTGCTCGCTCCATTg    <  2:2074720/93‑1 (MQ=255)
                                                                               aaGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATGAGCCAGATATGACCTACGTTGAATCGGCTGCTCGCTCCATTg    >  1:1248520/1‑93 (MQ=255)
                                                                                aGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATGAGCCAGATATGACCTACGTTGAATCGGCTGCTCGCTCCATTgc   <  2:5525578/93‑1 (MQ=255)
                                                                                aGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATGAGCCAGATATGACCTACGTTGAATCGGCTGCTCGCTCCATTgc   >  2:2699117/1‑93 (MQ=255)
                                                                                aGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATGAGCCAGATATGACCTACGTTGAATCGGCTGCTCGCTCCATTgc   <  1:6426368/93‑1 (MQ=255)
                                                                                aGCGGATGCCTGGCTGATTGCTGTACCCACGCAGTTTAAGGGCGATCATGAGCCAGATATGACCTACGTTGAATCGGCTGCTCGCTCCATTgc   <  1:2329332/93‑1 (MQ=255)
                                                                                 gCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATGAGCCAGATATGACCTACGTTGAATCGGCTGCTCGCTCCATTgcg  <  2:2273747/93‑1 (MQ=255)
                                                                                       ||                                                                                     
ATCGTCGAACCTGATTTGGCGAGTGTAGTAAAAACTGCCGTAGAAGGCGGTTTTTTACGAGCGAGCACGACGCCAGTTGAAGCGGATCGCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATGAGCCAGATATGACCTACGTTGAATCGGCTGCTCGCTCCATTGCG  >  NC_000913/3969016‑3969189

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
TATCGTCGAACCTGATTTGGCGAGTGTAGTAAAAACTGCCGTAGAAGGCGGTTTTTTACGAGCGAGCACGACGCCAGTTGAAGCGGATCGCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATGAGCCAGATATGACCTACGTTGAATCGGCTGCTCGCTCCATTGCGCCA  >  NC_000913/3969015‑3969192
                                                                                        |                                                                                         
TATCGTCGAACCTGATTTGGCGAGTGTAGTAAAAACTGCCGTAGAAGGCGGTTTTTTACGAGCGAGCACGACGCCAGTTGAAGCGGATGCCTGG                                                                                      <  SRR4302537.3087429/94‑1 (MQ=60)
TATCGTCGAACCTGATTTGGCGAGTGTAGTAAAAACTGCCGTAGAAGGCGGTTTTTTACGAGCGAGCACGACGCCAGTTGAAGCGGATGCCTGG                                                                                      <  SRR4302537.506409/94‑1 (MQ=60)
CATCGTCGAACCTGATTTGGCGAGTGTAGTAAAAACTGCCGTAGAAGGCGGTTTTTTAGGAGCGAGCACGACGCCAGTTGAAGCGGATGCCTGG                                                                                      <  SRR4302537.2873889/94‑1 (MQ=60)
 ATCGTCGACCCTGATTGGGCGAGTGTAGTAAAAACTGCCGTAGAAGGCGGTTTTTTACGAGCGAGCACGACGCCAGTTGAAGCGGATGCCTGGC                                                                                     <  SRR4302537.2675342/94‑1 (MQ=60)
    GTCGAACCTGATTTGGCGAGTGTAGTAAAAACTGCCGTAGAAGGCGGTTTTTTACGAGCGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGA                                                                                  >  SRR4302537.3178633/1‑94 (MQ=60)
      CGAACCTGATTTGGCGAGTGTAGTAAAAACTGCCGTAGAAGGCGGTTTTTTACGAGCGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGATT                                                                                <  SRR4302537.6006794/94‑1 (MQ=60)
       GAACCTGATTTGGCGAGTGTAGTAAAAACTGCCGTAGAAGGCGGTTTTTTACGAGCGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTG                                                                               >  SRR4302537.1100434/1‑94 (MQ=60)
        AACCTGATTTGGCGAGTGTAGTAAAAACTGCCGTAGAAGGCGGTTTTTTACGAGCGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGC                                                                              >  SRR4302537.3734634/1‑94 (MQ=60)
         ACCTGATTTGGCGAGTGTAGTAAAAACTGCCGTAGAAGTCGGTTTTTTACGAGCGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCT                                                                             <  SRR4302537.811113/94‑1 (MQ=60)
         ACCTGATTTGGCGAGTGTAGTAAAAACTGCCGTAGAAGGCGGTTTTTTACGAGCGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCT                                                                             <  SRR4302537.3848750/94‑1 (MQ=60)
          ccgggttgggctagtgtagaaaaccgTGCCGTAGAAGTCGTTTTTTTACGAGCGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTG                                                                            <  SRR4302537.5752293/68‑1 (MQ=60)
            TGATTTGGCGAGTGTAGTAAAAACTGCCGTAGAAGGCGGTTTTTTACGAGCGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTA                                                                          <  SRR4302537.3234453/94‑1 (MQ=60)
             GATTTGGCGAGTGTAGTAAAAACTGCCGTAGAAGGCGGTTTTTTACGAGCGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTAC                                                                         >  SRR4302537.1966216/1‑94 (MQ=60)
              ATTTGGCGAGTGTAGTAAAAACTGCCGTAGAAGGCGGTTTTTTACGAGCGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACC                                                                        <  SRR4302537.3780637/94‑1 (MQ=60)
              ATTGGGCGAGTGTAGT‑AAAGCTGCCGTAGAAGTCGGTTTTTTACGAGCGATCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCC                                                                       <  SRR4302537.3118601/94‑1 (MQ=60)
               TTTGGCGAGTGTAGTAAAAACTGCCGTAGAAGGCGGTTTTTTACGAGCGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCC                                                                       <  SRR4302537.6422238/94‑1 (MQ=60)
                TTGGCGAGTGTAGTAAAAACTGCGTAAGAAGGCGGTTTTTTACGAGCGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCA                                                                      <  SRR4302537.2642284/94‑1 (MQ=60)
                TTGGCGAGTGTAGTAAAAACTGCCGTAGAAGGCGGTTTTTTACGAGCGAGCACGACGCCAGTTTAAGCGGATGCCTGGCTGATTGCTGTACCCA                                                                      >  SRR4302537.5156809/1‑94 (MQ=60)
                 TGGCGAGTGTAGTAAAAACTGCCGTAGAAGGCGGTTTTTTACGAGCGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCAC                                                                     >  SRR4302537.1312697/1‑94 (MQ=60)
                     GAGTGTAGTAAAAACTGCCGTAGAAGGCGGTTTTTTACGAGCGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCG                                                                 >  SRR4302537.276310/1‑94 (MQ=60)
                      AGTGTAGTAAAAACTGCCGTAGAAGGCGGTTTTTTACGAGCGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGT                                                                >  SRR4302537.2956021/1‑94 (MQ=60)
                       GTGTAGTAAAAACTGCCGTAGAAGTCGGTTTTTTACGAGCGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTT                                                               <  SRR4302537.4269294/94‑1 (MQ=60)
                        TGTAGTAAAAACTGCCGTAGAAGGCGGTTTTTTACGAGCGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTT                                                              <  SRR4302537.3166883/94‑1 (MQ=60)
                          AAGTAAAAACTGCCGTAGAAGGCGGTTTTTTACGAGCTAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAA                                                            <  SRR4302537.2623849/94‑1 (MQ=60)
                           AGTAAAAACTGCCGTAGAAGGCGGTTTTTTACGAGCGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAG                                                           >  SRR4302537.929004/1‑94 (MQ=60)
                           AGTAAAAACTGCCGTAGAAGGCGGTTTTTTACGAGCGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAG                                                           <  SRR4302537.2043167/94‑1 (MQ=60)
                           AGTAAAAACTGCCGTAGAAGGCGGTTTTTTACGAGCGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAG                                                           <  SRR4302537.2089834/94‑1 (MQ=60)
                            GTAAAAACTGCCGTAGAAGGCGGTTTTTTACGAGCGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGG                                                          >  SRR4302537.25495/1‑94 (MQ=60)
                            GTAAAAACTGCCGTAGAAGGCGGTTTTTTACGAGCGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGG                                                          <  SRR4302537.2810487/94‑1 (MQ=60)
                             TAAAAACTGCCGTAGAAGGCGGTTTTTTACGAGCGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGG                                                         <  SRR4302537.3212607/94‑1 (MQ=60)
                               AAAACTGCCGTAGAGGGCGTTTTTTTACGAGCGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCG                                                       <  SRR4302537.3453842/94‑1 (MQ=60)
                               AAAACTGCCGTAGAAGGCGTTTTTTTACGAGCGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCG                                                       <  SRR4302537.5828866/94‑1 (MQ=60)
                               AAAACTGCCGTAGAAGGCGGTTTTTTACGAGCGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCG                                                       >  SRR4302537.4324971/1‑94 (MQ=60)
                                atcctgcgggAGAAGGCGGTTTTTTACGAGAGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGA                                                      <  SRR4302537.3520994/84‑1 (MQ=60)
                                 AACTGCCGTAGAAGGCGGTTTTTTACGAGCGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGAT                                                     >  SRR4302537.129834/1‑94 (MQ=60)
                                  ACTGCCGTAGAAGGCGGTTTTTTACGAGCGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATC                                                    >  SRR4302537.266280/1‑94 (MQ=60)
                                  ACTGCCGTAGAAGGCGGTTTTTTACGAGCGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATC                                                    <  SRR4302537.1501260/94‑1 (MQ=60)
                                  ACTGCCGTAGAAGGCGGTTTTTTACGAGCGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATC                                                    <  SRR4302537.3278330/94‑1 (MQ=60)
                                    TGCCGTAGAAGGCGGTTTTTTACGAGCGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCAT                                                  <  SRR4302537.6172298/94‑1 (MQ=60)
                                     GCCGTAGAAGGCGGTTTTTTACGAGCGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGACGTTTAAGGGCGATCATG                                                 >  SRR4302537.1794587/1‑94 (MQ=60)
                                     GCCGTAGAAGGCGGTTTTTTACGAGCGAACACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATG                                                 <  SRR4302537.2297266/94‑1 (MQ=60)
                                       CGTAGAGGGCGTTTTTTGACGAGCGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATGAG                                               <  SRR4302537.3493852/94‑1 (MQ=60)
                                       CGTAGAAGGCGGTTTTTTACGAGCGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATGAG                                               <  SRR4302537.5211831/94‑1 (MQ=60)
                                        GTAGAAGGCGGTTTTTTACGAGCGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATGAGC                                              >  SRR4302537.2118349/1‑94 (MQ=60)
                                        GTAGAAGGCGGTTTTTTACGAGCGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATGAGC                                              >  SRR4302537.4022406/1‑94 (MQ=60)
                                        GTAGAAGGCGGTTTTTTACGAGCGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATGAGC                                              >  SRR4302537.71743/1‑94 (MQ=60)
                                         TAGAAGGCGGTTTTTTACGAGCGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTAGCCACGCCGTTTAAGGGCGATCATGAGCC                                             >  SRR4302537.2837195/1‑94 (MQ=60)
                                         TAGAAGGCGGCTTTTTACGAGCGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATGAGCC                                             >  SRR4302537.5112429/1‑94 (MQ=60)
                                          AGAAGGCGGTTTTTTACGAGCGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATGAGCCA                                            >  SRR4302537.6336849/1‑94 (MQ=60)
                                           GAGGGCGTTTTTTTACGAGCGCGCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATGAGCCAG                                           <  SRR4302537.4433135/94‑1 (MQ=60)
                                           GAGGGCGGTTTTTTACGAGCGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATGAGCCAG                                           <  SRR4302537.3784045/94‑1 (MQ=60)
                                             AGGCGGTTTTTTACGAGCGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATGAGCCAGAT                                         <  SRR4302537.307477/94‑1 (MQ=60)
                                             AGGCGGTTTTTTACGAGCGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATGAGCCAGAT                                         <  SRR4302537.4232479/94‑1 (MQ=60)
                                             AGGCGGTTTTTTACGAGCGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATGAGCCAGAT                                         <  SRR4302537.5696899/94‑1 (MQ=60)
                                              GGCGGTTTTTTACGAGCGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATGAGCCAGATA                                        >  SRR4302537.533782/1‑94 (MQ=60)
                                               GCGGTTTTTTACGAGCGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATGAGCCAGATAT                                       >  SRR4302537.3470540/1‑94 (MQ=60)
                                               GCGGTTTTTTACGAGCGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATGAGCCAGATAT                                       <  SRR4302537.4255196/94‑1 (MQ=60)
                                               GCGGTTTTTTACGAGCGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATGAGCCAGATAT                                       >  SRR4302537.1432394/1‑94 (MQ=60)
                                               GCGGTTTTTTACGAGCGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATAAGCCAGATAT                                       <  SRR4302537.6269853/94‑1 (MQ=60)
                                               GCGGTTTTTGACGAGCGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATGAGCCAGATAT                                       <  SRR4302537.1345709/94‑1 (MQ=60)
                                                CGGTTTTTTACGAGCGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATGAGCCAGATATG                                      <  SRR4302537.1619983/94‑1 (MQ=60)
                                                 GGTTTTTTACGAGCGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATGAGCCAGATATGA                                     <  SRR4302537.920826/94‑1 (MQ=60)
                                                 GGTTTTTTACGAGCGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATGAGCCAGATATGA                                     <  SRR4302537.1465941/94‑1 (MQ=60)
                                                   TTTTTTACGAGCGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATGAGCCAGATATGACC                                   <  SRR4302537.4933936/94‑1 (MQ=60)
                                                   TTTTTTACGAGCGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATGAGCCAGATATGACC                                   >  SRR4302537.5962030/1‑94 (MQ=60)
                                                    TTTTTACGAGCGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATGAGCCAGATATGACCT                                  <  SRR4302537.2990746/94‑1 (MQ=60)
                                                      TTTACGAGCGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATGAGCCAGATATGACCTAC                                <  SRR4302537.2577380/94‑1 (MQ=60)
                                                        TGCGAGCGAGCACGACGCCGGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATGAGCCAGATATGACCTACGT                              <  SRR4302537.1129694/94‑1 (MQ=60)
                                                        TACGAGCGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATGAGCCAGATATGACCTACGT                              <  SRR4302537.5374114/94‑1 (MQ=60)
                                                         ACGAGCGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATGAGCCAGATATGACCTACGTT                             <  SRR4302537.852518/94‑1 (MQ=60)
                                                          CGAGCGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATGAGCCAGATATGACCTACGTTG                            <  SRR4302537.5245618/94‑1 (MQ=60)
                                                           GAGCGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATGAGCCAGATATGACCTACGTTGA                           >  SRR4302537.2820830/1‑94 (MQ=60)
                                                           GAGAGACCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATGAGCCAGATATGACCTACGTTGA                           <  SRR4302537.4456159/94‑1 (MQ=60)
                                                            AGCGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATGAGCCAGATATGACCTACGTTGAA                          >  SRR4302537.4412739/1‑94 (MQ=60)
                                                            AGCGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATGAGCCAGATATGACCTACGTTGAA                          <  SRR4302537.6348021/94‑1 (MQ=60)
                                                             GCGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATGAGCCAGATATGACCTACGTTGAAT                         <  SRR4302537.3741154/94‑1 (MQ=60)
                                                             GCGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATGAGCCAGATATGACCTACGTTGAAT                         <  SRR4302537.3521348/94‑1 (MQ=60)
                                                              CGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATGAGCCAGATATGACCTACGTTGAATC                        <  SRR4302537.4349065/94‑1 (MQ=60)
                                                              CGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATGAGCCAGATATGACCTACGTTGAATC                        <  SRR4302537.2752537/94‑1 (MQ=60)
                                                               GAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATGAGCCAGATATGACCTACGTTGAATCG                       <  SRR4302537.301979/94‑1 (MQ=60)
                                                               GAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATGAGCCAGATATGACCTACGTTGAATCG                       <  SRR4302537.1305395/94‑1 (MQ=60)
                                                               GAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATGAGCCAGATATGACCTACGTTGAATCG                       >  SRR4302537.3376790/1‑94 (MQ=60)
                                                                AGCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATGAGCCAGATATGACCTACGTTGAATCGG                      <  SRR4302537.478254/94‑1 (MQ=60)
                                                                AGCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATGAGCCAGATATGACCTACGTTGAATCGG                      <  SRR4302537.4905142/94‑1 (MQ=60)
                                                                AGCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATGAGCCAGATATGACCTACGTTGAATCGG                      >  SRR4302537.2326973/1‑94 (MQ=60)
                                                                AGCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATGAGCCAGATATGACCTACGTTGAATCGG                      >  SRR4302537.3662109/1‑94 (MQ=60)
                                                                 GCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATGAGCCAGATATGACCTACGTTGAATCGGC                     <  SRR4302537.1438758/94‑1 (MQ=60)
                                                                  CACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATGAGCCAGATATGACCTACGTTGAATCGGCT                    <  SRR4302537.4125851/94‑1 (MQ=60)
                                                                  CACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATGAGCCAGATATGACCTACGTTGAATCGGCT                    <  SRR4302537.3648439/94‑1 (MQ=60)
                                                                  CACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATGAGCCAGATATGACCTACGTTGAATCGGCT                    <  SRR4302537.377374/94‑1 (MQ=60)
                                                                   ACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATGAGCCAGATATGACCTACGTTGAATCGGCTG                   <  SRR4302537.1843683/94‑1 (MQ=60)
                                                                   ACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATGAGCCAGATATGACCTACGTTGAATCGGATG                   <  SRR4302537.3136299/94‑1 (MQ=60)
                                                                     GACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATGAGCCAGATATGACCTACGTTGAATCGGCTGCT                 <  SRR4302537.6484664/94‑1 (MQ=60)
                                                                        GCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATGAGCCAGATATGACCTACGTTGAATCGGCTGCTCGC              >  SRR4302537.6164254/1‑94 (MQ=60)
                                                                          CAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATGAGCCAGATATGACCTACGTTGAATCGGCTGCTCGCTC            >  SRR4302537.6089215/1‑94 (MQ=60)
                                                                             TTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATGAGCCAGATATGACCTACGTTGAATCGGCTGCTCGCTCCAT         <  SRR4302537.2473537/94‑1 (MQ=60)
                                                                              TGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATGAGCCAGATATGACCTACGTTGAATCGGCTGCTCGCTCCATT        <  SRR4302537.1766937/94‑1 (MQ=60)
                                                                               GAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATGAGCCAGATATGACCTACGTTGAATCGGCTGCTCGCTCCATTG       <  SRR4302537.5795833/94‑1 (MQ=60)
                                                                               GAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATGAGCCAGATATGACCTACGTTGAATCGGCTGCTCGCTCCATTG       >  SRR4302537.1257216/1‑94 (MQ=60)
                                                                                AAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATGAGCCAGATATGACCTACGTTGAATCGGCTGCTCGCTCCATTGC      >  SRR4302537.5921683/1‑94 (MQ=60)
                                                                                 AGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAATGGCGATCATGAGCCAGATATGACCTACGTTGAATCGGCTGCTCGCTCCATTGCG     <  SRR4302537.5458457/94‑1 (MQ=60)
                                                                                 AGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATGAGCCAGATATGACCTACGTTGAATCGGCTGCTCGCTCCATTGCG     >  SRR4302537.1931281/1‑94 (MQ=60)
                                                                                 AGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATGAGCCAGATATGACCTACGTTGAATCGGCTGCTCGCTCCATTGCG     <  SRR4302537.6470034/94‑1 (MQ=60)
                                                                                 AGCGGATGCCTGGCTGATTGCTGTACCCACGCAGTTTAAGGGCGATCATGAGCCAGATATGACCTACGTTGAATCGGCTGCTCGCTCCATTGCG     <  SRR4302537.2345760/94‑1 (MQ=60)
                                                                                   CGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATGAGCCAGATATGACCTACGTTGAATCGGCTGCTCGCTCCATTGCGCC   >  SRR4302537.2233685/1‑94 (MQ=60)
                                                                                    GGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATGAGCCAGATATGACCTACGTTGAATCGGCTGCTCGCTCCATTGCGCCA  >  SRR4302537.1226875/1‑94 (MQ=60)
                                                                                        |                                                                                         
TATCGTCGAACCTGATTTGGCGAGTGTAGTAAAAACTGCCGTAGAAGGCGGTTTTTTACGAGCGAGCACGACGCCAGTTGAAGCGGATCGCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATGAGCCAGATATGACCTACGTTGAATCGGCTGCTCGCTCCATTGCGCCA  >  NC_000913/3969015‑3969192

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 2 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑