Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I2 R1 882 254.4 12957588 98.0% 12698436 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,971,075 2 bp→GC coding (942‑943/1068 nt) rffG → dTDP‑glucose 4,6‑dehydratase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,971,0750CG100.0% 702.1 / NA 203C314W (TGC→TGG) rffGdTDP‑glucose 4,6‑dehydratase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base G (114/89);  total (114/89)
*NC_0009133,971,0760GC100.0% 709.6 / NA 203V315L (GTG→CTG) rffGdTDP‑glucose 4,6‑dehydratase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base C (114/89);  total (114/89)

Only 100 of 209 total aligned reads displayed.

GACCTGATCACCTTTGTCGCTGACCGTCCGGGGCATGATCTGCGTTATGCCATTGATGCTTCGAAAATTGCCCGTGAACTTGGCTGCGTGCCGCAGGAAACCTTTGAAAGTGGAATGCGTAAAACGGTTCAGTGGTATCTGGCTAATGAAAGCTGGTGGAAGCAGGTGCAGGA  >  NC_000913/3970989‑3971161
                                                                                      ||                                                                                     
 aCCTGATCACCTTTGTCGCTGACCGTCCGGGGCATGATCTGCGTTATGCCATTGATGCTTCGAAAATTGCCCGTGAACTTGGCTGGCTGCCGc                                                                                 >  1:2916118/1‑93 (MQ=255)
 aCCTGATCACCTTTGTCGCTGACCGTCCGGGGCATGATCTGCGTTATGCCATTGATGCTTCGAAAATTGCCAGTGAACTTGGCTGGCTGcccg                                                                                 >  1:1472182/1‑91 (MQ=255)
   cTGATCACCTTTGTCGCTGACCGTCCGGGGCATGATCTGCGTTATGCCATTGATGCTTCGAAAATTGCCCGTGAACTTGGCTGGCTGCCGCAg                                                                               <  2:2273504/93‑1 (MQ=255)
   cTGATCACCTTTGTCGCTGACCGTCCGGGGCATGATCTGCGTTATGCCATTGATGCTTCGAAAATTGCCCGTGAACTTGGCTGGCTGCCGCAg                                                                               >  2:6458020/1‑93 (MQ=255)
   cTGATCACCTTTGTCGCTGACCGTCCGGGGCATGATCTGCGTTATGCCATTGATGCTTCGAAAATTGCCCGTGAACTTGGCTGGCTGCCGCAg                                                                               >  2:3318628/1‑93 (MQ=255)
    tGATCACCTTTGTCGCTGACCGTCCGGGGCATGATCTGCGTTATGCCATTGATGCTTCGAAAATTGCCCGTGAACTTGGCTGGCTGCCGCAgg                                                                              >  1:1048164/1‑93 (MQ=255)
     gATCACCTTTGTCGCTGACCGTCCGGGGCATGATCTGCGTTATGCCATTGATGCTTCGAAAATTGCCCGTGAACTTGGCTGGCTGCCGCAGGa                                                                             <  2:2581330/93‑1 (MQ=255)
     gATCACCTTTGTCGCTGACCGTCCGGGGCATGATCTGCGTTATGCCATTGATGCTTCGAAAATTGCCCGTGAACTTGGCTGGCTGCCGCAGGa                                                                             <  1:5742726/93‑1 (MQ=255)
     gATCACCTTTGTCGCTGACCGTCCGGGGCATGATCTGCGTTATGCCATTGATGCTTCGAAAATTGCCCGTGAACTTGGCTGGCTGCCGCAGGa                                                                             >  1:2212557/1‑93 (MQ=255)
       tCACCTTTGTCGCTGACCGTCCGGGGCATGATCTGCGTTATGCCATTGATGCTTCGAAAATTGCCCGTGAACTTGGCTGGCTGCCGCAGGaaa                                                                           <  2:5556272/93‑1 (MQ=255)
        cACCTTTGTCGCTGACCGTCCGGGGCATGATCTGCGTTATGCCATTGATGCTTCGAAAATTGCCCGTGAACTTGGCTGGCTGCCGCAGGAAAc                                                                          <  1:479886/93‑1 (MQ=255)
        cACCTTTGTCGCTGACCGTCCGGGGCATGATCTGCGTTATGCCATTGATGCTTCGAAAATTGCCCGTGAACTTGGCTGGCTGCCGCAGGAAAc                                                                          >  2:2457279/1‑93 (MQ=255)
         aCCTTTGTCGCTGACCGTCCGGGGCATGATCTGCGTTATGCCATTGATGCTTCGAAAATTGCCCGTGAACTTGGCTGGCTGCCGCAGGAAAcc                                                                         <  2:765440/93‑1 (MQ=255)
          ccTTTGTCGCTGACCGTCCGGGGCATGATCTGCGTTATGCCATTGATGCTTCGAAAATTGCCCGTGAACTTGGCTGGCTGCCGCAGGAAACCt                                                                        >  1:1269594/1‑93 (MQ=255)
          ccTTTGTCGCTGACCGTCCGGGGCATGATCTGCGTTATGCCATTGATGCTTCGAAAATTGCCCGTGAACTTGGCTGGCTGCCGCAGGAAACCt                                                                        >  1:2348663/1‑93 (MQ=255)
           cTTTGTCGCTGACCGTCCGGGGCATGATCTGCGTTATGCCATTGATGCTTCGAAAATTGCCCGTGAACTTGGCTGGCTGCCGCAGGAAACCtt                                                                       <  1:1076603/93‑1 (MQ=255)
             ttGTCGCTGACCGTCCGGGGCATGATCTGCGTTATGCCATTGATGCTTCGAAAATTGCCCGTGAACTTGGCTGGCTGCCGCAGGAAACCTTTg                                                                     >  1:2694581/1‑93 (MQ=255)
             ttGTCGCTGACCGTCCGGGGCATGATCTGCGTTATGCCATTGATGCTTCGAAAATTGCCCGTGAACTTGGCTGGCTGCCGCAGGAAACCTTTg                                                                     <  2:313167/93‑1 (MQ=255)
                  gCTGACCGTCCGGGGCATGATCTGCGTTATGCCATTGATGCTTCGAAAATTGCCCGTGAACTTGGCTGGCTGCCGCAGGAAACCTTTGAAAgg                                                                >  1:4141970/1‑92 (MQ=255)
                  gCTGACCGTCCGGGGCATGATCTGCGTTATGCCATTGATGCTTCGAAAATTGCCCGTGAACTTGGCTGGCTGCCGCAGGAAACCTTTGAAAGt                                                                >  2:2646682/1‑93 (MQ=255)
                   cTGACCGTCCGGGGCATGATCTGCGTTATGCCATTGATGCTTCGAAAATTGCCCGTGAACTTGGCTGGCTGCCGCAGGAAACCTTTGAAAGTg                                                               <  2:156892/93‑1 (MQ=255)
                   cTGACCGTCCGGGGCATGATCTGCGTTATGCCATTGATGCTTCGAAAATTGCCCGTGAACTTGGCTGGCTGCCGCAGGAAACCTTTGAAAGTg                                                               >  2:4476691/1‑93 (MQ=255)
                    tGACCGTCCGGGGCATGATCTGCGTTATGCCATTGATGCTTCGAAAATTGCCCGTGAACTTGGCTGGCTGCCGCAGGAAACCTTTGAAAGTgg                                                              >  1:5266154/1‑93 (MQ=255)
                    tGACCGTCCGGGGCATGATCTGCGTTATGCCATTGATGCTTCGAAAATTGCCCGTGAACTTGGCTGGCTGCCGCAGGAAACCTTTGAAAGTgg                                                              >  2:5203469/1‑93 (MQ=255)
                     gACCGTCCGGGGCATGATCTGCGTTATGCCATTGATGCTTCGAAAATTGCCCGTGAACTTGGCTGGCTGCCGCAGGAAACCTTTGAAAGTGGa                                                             <  2:3712331/93‑1 (MQ=255)
                     gACCGTCCGGGGCATGATCTGCGTTATGCCATTGATGCTTCGAAAATTGCCCGTGAACTTGGCTGGCTGCCGCAGGAAACCTTTGAAAGTGGa                                                             >  1:6047294/1‑93 (MQ=255)
                       ccGTCCGGGGCATGATCTGCGTTATGCCATTGATGCTTCGAAAATTGCCCGTGAACTTGGCTGGCTGCCGCAGGAAACCTTTGAAAGTGGAAt                                                           <  1:5852575/93‑1 (MQ=255)
                         gTCTGGGGAATGATCTGCGTTATGCCATTGATGCTTCGAAAATTGCCCGTGAACTTGGCTGGCTGCCGCAGGAAACCTTTGAAAGTGGAATGc                                                         <  1:3474281/93‑1 (MQ=255)
                         gTCCGGGGCATGATCTGCGTTATGCCATTGATGCTTCGAAAATTGCCCGTGAACTTGGCTGGCTGCCGCAGGAAACCTTTGAAAGTGGAATGc                                                         >  1:186963/1‑93 (MQ=255)
                           ccGGGGCATGATCTGCGTTATGCCATTGATGCTTCGAAAATTGCCCGTGAACTTGGCTGGCTGCCGCAGGGAACCTTTGAAAGTGGAATGCGt                                                       >  2:271879/1‑93 (MQ=255)
                           ccGCGGCATGATCTCCGTTATGGCATTGAAGCTGCGAAAATTGCCCGTGAACTTGGCTGGCTGCCGCAGGAACCCTGTGAAAGTGGAATGCGt                                                       <  2:1944150/93‑1 (MQ=255)
                             ggggCATGATCTGCGTTATGCCATTGATGCTTCGAAAATTGCCCGTGAACTTGGCTGGCTGCCGCAGGAAACCTTTGAAAGTGGAATGCGTaa                                                     >  1:1925610/1‑93 (MQ=255)
                               ggCATGATCTGCGTTATGCCATTGATGCTTCGAAAATTGCCCGTGAACTTGGCTGGCTGCCGCAGGAAACCTTTGAAAGTGGAATGCGTaaaa                                                   >  1:2436881/1‑93 (MQ=255)
                                gCATGATCTGCGTTATGCCATTGATGCTTCGAAAATTGCCCGTGAACTTGGCTGGCTGCCGCAGGAAACCTTTGAAAGTGGAATGCGTAAAAc                                                  >  2:5575069/1‑93 (MQ=255)
                                gCATGATCTGCGTTATGCCATTGATGCTTCGAAAATTGCCCGTGAACTTGGCTGGCTGCCGCAGGAAACCTTTGAAAGTGGAATGCGTAAAAc                                                  <  1:3007110/93‑1 (MQ=255)
                                 cATGATCTGCGTTATGCCATTGATGCTTCGAAAATTGCCCGTGAACTTGGCTGGCTGCCGCAGGAAACCTTTGAAAGTGGAATGCGTAAAACg                                                 <  2:4141970/93‑1 (MQ=255)
                                  aTGATCTGCGTTATGCCATTGATGCTTCGAAAATTGCCCGTGAACTTGGCTGGCTGCCGCAGGAAACCTTTGAAAGTGGAATGCGTAAAACgg                                                >  2:4682770/1‑93 (MQ=255)
                                   tGATCTGCGTTATGCCATTGATGCTTCGAAAATTGCCCGTGAACTTGGCTGGCTGCCGCAGGAAACCTTTGAAAGTGGAATGCGTAAAACGGt                                               <  2:6216507/93‑1 (MQ=255)
                                   tGATCTGCGTTATGCCATTGATGCTTCGAAAATTGCCCGTGAACTTGGCTGGCTGCCGCAGGAAACCTTTGAAAGTGGAATGCGTAAAACGGt                                               <  1:537029/93‑1 (MQ=255)
                                    gATCTGCGTTATGCCATTGATGCTTCGAAAATTGCCCGTGAACTTGGCTGGCTGCCGCAGGAAACCTTTGAAAGTGGAATGCGTAAAACGGtt                                              <  2:6403740/93‑1 (MQ=255)
                                    gATCTGCGTTATGCCATTGATGCTTCGAAAATTGCCCGTGAACTTGGCTGGCTGCCGCAGGAAACCTTTGAAAGTGGAATGCGTAAAACGGtt                                              >  1:2833200/1‑93 (MQ=255)
                                     aTCTGCGTTATGCCATTGATGCTTCGAAAATTGCCCGTGAACTTGGCTGGCTGCCGCAGGAAACCTTTGAAAGTGGAATGCGTAAAACGGTTc                                             <  1:1522025/93‑1 (MQ=255)
                                     aTCTGCGTTATGCCATTGATGCTTCGAAAATTGCCCGTGAACTTGGCTGGCTGCCGCAGGAAACCTTTGAAAGTGGAATGCGTAAAACGGTTc                                             >  1:811537/1‑93 (MQ=255)
                                     aTCTGCGTTATGCCATTGATGCTTCGAAAATTGCCCGTGAACTTGGCTGGCTGCCGCAGGAAACCTTTGAAAGTGGAATGCGTAAAACGGTTc                                             >  2:4271313/1‑93 (MQ=255)
                                     aTCTGCGTTATGCCATTGATGCTTCGAAAATTGCCCGTGAACTTGGCTGGCTGCCGCAGGAAACCTTTGAAAGTGGAATGCGTAAAACGGTTc                                             >  2:3928152/1‑93 (MQ=255)
                                      tCTGCGTTATGCCATTGATGCTTCGAAAATTGCCCGTGAACTTGGCTGGCTGCCGCAGGAAACCTTTGAAAGTGGAATGCGTAAAACGGTTCa                                            >  1:3979703/1‑93 (MQ=255)
                                      tCTGCGTTATGCCATTGATGCTTCGAAAATTGCCCGTGAACTTGGCTGGCTGCCGCAGGAAACCTTTGAAAGTGGAATGCGTAAAACGGTTCa                                            >  1:1223735/1‑93 (MQ=255)
                                       cTGCGTTATGCCATTGATGCTTCGAAAATTGCCCGTGAACTTGGCTGGCTGCCGCAGGAAACCTTTGAAAGTGGAATGCGTAAAACGGTTCAg                                           <  2:4099361/93‑1 (MQ=255)
                                        tGCGTTATGCCATTGATGCTTCGAAAATTGCCCGTGAACTTGGCTGGCTGCCGCAGGAAACCTTTGAAAGTGGAATGCGTAAAACGGTTCAGt                                          <  2:5351469/93‑1 (MQ=255)
                                         gCGTTATGCCATTGATGCTTCGAAAATTGCCCGTGAACTTGGCTGGCTGCCGCAGGAAACCTTTGAAAGTGGAATGCGTAAAACGGTTCAGTg                                         >  1:4220110/1‑93 (MQ=255)
                                          cGTTATGCCATTGATGCTTCGAAAAGTGCCCGTGAACTTGGCTGGCTGCCGCAGGAAACCTTTGAAAGTGGAATGCGTAAAACGGTTCAGTgg                                        <  1:6009677/93‑1 (MQ=255)
                                            ttATGCCATTGATGCTTCGAAAATTGCCCGTGAACTTGGCTGGCTGCCGCAGGAAACCTTTGAAAGTGGAATGCGTAAAACGGTTCAGTGGTa                                      >  1:897504/1‑93 (MQ=255)
                                             tATGCCATTGATGCTTCGAAAATTGCCCGTGAACTTGGCTGGCTGCCGCAGGAAACCTTTGAAAGTGGAATGCGTAAAACGGTTCAGTGGTAt                                     >  1:2068796/1‑93 (MQ=255)
                                              aTGCCATTGATGCTTCGAAAATTGCCCGTGAACTTGGCTGGCTGCCGCAGGAAACCTTTGAAAGTGGAATGCGTAAAACGGTTCAGTGGTATc                                    <  2:5624460/93‑1 (MQ=255)
                                              aTGCCATTGATGCTTCGAAAATTGCCCGTGAACTTGGCTGGCTGCCGCAGGAAACCTTTGAAAGTGGAATGCGTAAAACGGTTCAGTGGTATc                                    >  2:330255/1‑93 (MQ=255)
                                              aTGCCATTGATGCTTCGAAAATTGCCCGTGAACTTGGCTGGCTGCCGCAGGAAACCTTTGAAAGTGGAATGCGTAAAACGGTTCAGTGGTATc                                    <  2:2152592/93‑1 (MQ=255)
                                               tGCCATTGATGCTTCGAAAATTGCCCGTGAACTTGGCTGGCTGCCGCAGGAAACCTTTGAAAGTGGAATGCGTAAAACGGTTCAGTGGTATCt                                   >  1:2033913/1‑93 (MQ=255)
                                                  cATTGATGCTTCGAAAATTGCCCGTGAACTTGGCTGGCTGCCGCAGGAAACCTTTGAAAGTGGAATGCGTAAAACGGTTCAGTGGTATCTGGc                                >  1:3996051/1‑93 (MQ=255)
                                                   aTTGATGCTTCGAAAATTGCCCGTGAACTTGGCTGGCTGCCGCAGGAAACCTTTGAAAGTGGAATGCGTAAAACGGTTCAGTGGTATCTGGCt                               >  1:762422/1‑93 (MQ=255)
                                                   aTTGATGCTTCGAAAATTGCCCGTGAACTTGGCTGGCTGCCGCAGGAAACCTTTGAAAGGGGCATGCGGAAAACGGTTCAGTGGTATCTGGCt                               >  1:1719081/1‑93 (MQ=255)
                                                   aTTGATGCTTCGAAAATTGCCCGTGAACTTGGATGGCTGCCGCAGGAAACCTTTGAAAGTGGAATGCGTAAAACGGTTCAGTGGTATCTGGCt                               >  1:3077775/1‑93 (MQ=255)
                                                    ttGATGCTTCGAAAATTGCCCGTGAACTTGGCTGGCTGCCGCAGGAAACCTTTGAAAGTGGAATGCGTAAAACGGTTCAGTGGTATCTGGCTa                              >  2:5451671/1‑93 (MQ=255)
                                                    ttGATGCTTCGAAAATTGCCCGTGAACTTGGCTGGCTGCCGCAGGAAACCTTTGAAAGTGGAATGCGTAAAACGGTTCAGTGGTATCTGGCTa                              >  2:1772781/1‑93 (MQ=255)
                                                     tGATGCTTCGAAAATTGCCCGTGAACTTGGCTGGCTGCCGCAGGAAACCTTTGAAAGTGGAATGCGTAAAACGGTTCAGTGGTATCTGGCTaa                             <  1:3650244/93‑1 (MQ=255)
                                                     tGATGCTTCGAAAATTGCCCGTGAACTTGGCTGGCTGCCGCAGGAAACCTTTGAAAGTGGAATGCGTAAAACGGTTCAGTGGTATCTGGCTaa                             >  1:5135934/1‑93 (MQ=255)
                                                        tGCTTCGAAAATTGCCCGTGAACTTGGCTGGCTGCCGCAGGAAACCTTTGAAAGTGGAATGCGTAAAACGGTTCAGTGGTATCTGGCTAATGa                          >  2:4977106/1‑93 (MQ=255)
                                                        tGCTTCGAAAATTGCCCGTGAACTTGGCTGGCTGCCGCAGGAAACCTTTGAAAGTGGAATGCGTAAAACGGTTCAGTGGTATCTGGCTAATGa                          >  1:635825/1‑93 (MQ=255)
                                                         gCTTCGAAAATTGCCCGTGAACTTGGCTGGCTGCCGCAGGAAACCTTTGAAAGTGGAATGCGTAAAACGGTTCAGTGGTATCTGGCTAATGaa                         >  1:5583090/1‑93 (MQ=255)
                                                            tCGAAAATTGCCCGTGAACTTGGCTGGCTGCCGCAGGAAACCTTTGAAAGTGGAATGCGTAAAACGGTTCAGTGGTATCTGGCTAATGAAAGc                      >  2:2363106/1‑93 (MQ=255)
                                                            tCGAAAATTGCCCGTGAACTTGGCTGGCTGCCCCAGGAAACCTTTGAAATTGGAATGCGTAAAACGGTCCAGTGGTATTTGGCTATTGAAAgt                      >  2:1418435/1‑92 (MQ=255)
                                                             cGAAAATTGCCCGTGAACTTGGCTGGCTGCCGCAGGAAACCTTTGAAAGTGGAATGCGTAAAACGGTTCAGTGGTATCTGGCTAATGAAAGCt                     >  1:3833722/1‑93 (MQ=255)
                                                              gAAAATTGCCCGTGAGCTTGGCTGGCTGCCGCAGGAAACCTTTGAAAGTGGAATGCGTAAAACGGTTCAGTGGTATCTGGCTAATGAAAGCtg                    <  2:2772952/93‑1 (MQ=255)
                                                              gAAAATTGCCCGTGAACTTGGCTGGCTGCCGCAGGAAACCTTTGAAAGTGGAATGCGTAAAACGGTTCAGTGGTATCTGGCTAATGAAAGCtg                    >  2:3517468/1‑93 (MQ=255)
                                                              gAAAATTGCCCGTGAACTTGGCTGGCTGCCGCAGGAAACCTTTGAAAGTGGAATGCGTAAAACGGTTCAGTGGTATCTGGCTAATGAAAGCtg                    >  1:2336306/1‑93 (MQ=255)
                                                              gAAAATTGCCCGTGAACTTGGCTGGCTGCCGCAGGAAACCTTTGAAAGTGGAATGCGTAAAACGGTTCAGTGGTATCTGGCTAATGAAAGCtg                    >  1:5066471/1‑93 (MQ=255)
                                                               aaaaTTGCCCGTGAACTTGGCTGGCTGCCGCAGGAAACCTTTGAAAGTGGAATGCGTAAAACGGTTCAGTGGTATCTGGCTAATGAAAGCtgg                   >  1:4282078/1‑93 (MQ=255)
                                                               aaaaTTGCCCGTGAACTTGGCTGGCTGCCGCAGGAAACCTTTGAAAGTGGAATGCGTAAAACGGTTCAGTGGTATCTGGCTAATGAAAGCtgg                   <  1:6241508/93‑1 (MQ=255)
                                                                 aaTTGCCCGTGAACTTGGCTGGCTGCCGCAGGAAACCTTTGAAAGTGGAATGCGTAAAACGGTTCAGTGGTATCTGGCTAATGAAAGCtggtg                 >  2:2854857/1‑93 (MQ=255)
                                                                  aTTGCCCGTGAACTTGGCTGGCTGCCGCAGGAAACCTTTGAAAGTGGAATGCGTAAAACGGTTCAGTGGTATCTGGCTAATGAAAGCtggtgg                >  1:5667469/1‑93 (MQ=255)
                                                                   ttGCCCGTGAACTTGGCTGGCTGCCGCAGGAAACCTTTGAAAGTGGAATGCGTAAAACGGTTCAGTGGTATCTGGCTAATGAAAGCTGGTGGa               >  2:3196957/1‑93 (MQ=255)
                                                                   ttGCCCGTGAACTTGGCTGGCTGCCGCAGGAAACCTTTGAAAGTGGAATGCGTAAAACGGTTCAGTGGTATCTGGCTAATGAAAGCTGGTGGa               >  1:3693071/1‑93 (MQ=255)
                                                                    tGCCCGTGAACTTGGCTGGCTGCCGCAGGAAACCTTTGAAAGTGGAATGCGTAAAACGGTTCAGTGGTATCTGGCTAATGAAAGCTGGTGGaa              <  2:5387920/93‑1 (MQ=255)
                                                                      cccGTGAACTTGGCTGGCTGCCGCAGGAAACCTTTGAAAGTGGAATGCGTAAAACGGTTCAGTGGTATCTGGCTAATGAAAGCTGGTGGAAGc            >  2:6118468/1‑93 (MQ=255)
                                                                      cccGTGAACTTGGCTGGCTGCCGCAGGAAACCTTTGAAAGTGGAATGCGTAAAACGGTTCAGTGGTATCTGGCTAATGAAAGCTGGTGGAAGc            <  1:5335961/93‑1 (MQ=255)
                                                                      cccGTGAACTTGGCTGGCTGCCGCAGGAAACCTTTGAAAGTGGAATGCGTAAAACGGTTCAGTGGTATCTGGCTAATGAAAGCTGGTGGAAGc            >  2:3650588/1‑93 (MQ=255)
                                                                       ccGTGAACTTGGCTGGCTGCCGCAGGAAACCTTTGAAAGTGGAATGCGTAAAACGGTTCAGTGGTATCTGGCTAATGAAAGCTGGTGGAAGCa           >  2:1258280/1‑93 (MQ=255)
                                                                        cGTGAACTTGGCTGGCTGCCGCAGGAAACCTTTGAAAGTGGAATGCGTAAAACGGTTCAGTGGTATCTGGCTAATGAAAGCTGGTGGAAGCAg          <  1:4731905/93‑1 (MQ=255)
                                                                        cGTGAACTTGGCTGGCTGCCGCAGGAAACCTTTGAAAGTGGAATGCGTAAAACGGTTCAGTGGTATCTGGCTAATGAAAGCTGGTGGAAGCAg          >  1:992752/1‑93 (MQ=255)
                                                                        cGTGAACTTGGCTGGCTGCCGCAGGAAACCTTTGAAAGTGGAATGCGTAAAACGGTTCAGTGGTATCTGGCTAATGAAAGCTGGTGGAAGCAg          <  1:2700529/93‑1 (MQ=255)
                                                                         gTGAACTTGGCTGGCTGCCGCAGGAAACCTTTGAAAGTGGAATGCGTAAAACGGTTCAGTGGTATCTGGCTAATGAAAGCTGGTGGAAGCAgg         <  2:3832389/93‑1 (MQ=255)
                                                                         gTGAACTTGGCTGGCTGCCGCAGGAAACCTTTGAAAGTGGAATGCGTAAAACGGTTCAGTGGTATCTGGCTAATGAAAGCTGGTGGAAGCAgg         >  1:3587138/1‑93 (MQ=255)
                                                                         gTGAACTTGGCTGGCTGCCGCAGGAAACCTTTGAAAGTGGAATGCGTAAAACGGTTCAGTGGTATCTGGCTAATGAAAGCTGGTGGAAGCAgg         <  1:2618764/93‑1 (MQ=255)
                                                                          tGAACTTGGCTGGCTGCCGCAGGAAACCTTTGAAAGTGGAATGCGTAAAACGGTTCAGTGGTATCTGGCTAATGAAAGCTGGTGGAAGCAggg        >  1:4324241/1‑92 (MQ=255)
                                                                          tGAACTTGGCTGGCTGCCGCAGGAAACCTTTGAAAGTGGAATGCGTAAAACGGTTCAGTGGTATCTGGCTAATGAAAGCTGGTGGAAGCAGGt        <  2:937010/93‑1 (MQ=255)
                                                                          tGAACTTGGCTGGCTGCCGCAGGAAACCTTTGAAAGTGGAATGCGTAAAACGGTTCAGTGGTATCTGGCTAATGAAAGCTGGTGGAAGCAGGt        >  1:485087/1‑93 (MQ=255)
                                                                           gAACTTGGCTGGCTGCCGCAGGAAACCTTTGAAAGTGGAATGCGTAAAACGGTTCAGTGGTATCTGGCTAATGAAAGCTGGTGGAAGCAGGTg       >  2:5830656/1‑93 (MQ=255)
                                                                           gAACTTGGCTGGCTGCCGCAGGAAACCTTTGAAAGTGGAATGCGTAAAACGGTTCAGTGGTATCTGGCTAATGAAAGCTGGTGGAAGCAGGTg       <  1:3314074/93‑1 (MQ=255)
                                                                             aCTTGGCTGGCTGCCGCAGGAAACCTTTGAAAGTGGAATGCGTAAAACGGTTCAGTGGTATCTGGCTAATGAAAGCTGGTGGAAGCAGGGGcg     >  1:1293520/1‑92 (MQ=255)
                                                                             aCTTGGCTGGCTGCCGCAGGAAACCTTTGAAAGTGGAATGCGGAAAACGGTTCAGTGGCATCTGGCTAATGAAAGCTGGTGGAAGCAGGTGCa     >  2:5925550/1‑93 (MQ=255)
                                                                               ttGGCTGGCTGCCTCAGGAAACCTTTGAAAGTGGAATGCGTAAAACGGTTCAGTGGTATCTGGCTAATGAAAGCTGGTGGAAGCAGGTGCAgg   >  2:4723272/1‑93 (MQ=255)
                                                                                      ||                                                                                     
GACCTGATCACCTTTGTCGCTGACCGTCCGGGGCATGATCTGCGTTATGCCATTGATGCTTCGAAAATTGCCCGTGAACTTGGCTGCGTGCCGCAGGAAACCTTTGAAAGTGGAATGCGTAAAACGGTTCAGTGGTATCTGGCTAATGAAAGCTGGTGGAAGCAGGTGCAGGA  >  NC_000913/3970989‑3971161

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 28 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
CTATCGTGACCTGATCACCTTTGTCGCTGACCGTCCGGGGCATGATCTGCGTTATGCCATTGATGCTTCGAAAATTGCCCGTGAACTTGGCTGCGTGCCGCAGGAAACCTTTGAAAGTGGAATGCGTAAAACGGTTCAGTGGTATCTGGCTAATGAAAGCTGGTGGAAGCAGGTGCAGGAC  >  NC_000913/3970982‑3971162
                                                                                             |                                                                                       
CTATCGTGACCTGATCACCTTTGTCGCTGACCGTCCGGGGCATGATCTGCGTTATGCCATTGATGCTTCGAAAATTGCCCGTGAACTTGGCTGG                                                                                         >  SRR4302537.3855325/1‑94 (MQ=60)
     GTGACCTGATCACCTTTGTCGCTGACCGTCCGGGGCATGATCTGCGTTATGCCATTGATGCTTCGAAAATTGCCCGTGAACTTGGCTGGCTGCC                                                                                    <  SRR4302537.1509666/94‑1 (MQ=60)
      TGACCTGATCACCTTTGTCGCTGACCGTCCGGGGCATGATCTGCGTTATGCCATTGATGCTTCGAAAATTGCCCGTGAACTTGGCTGGCTGCCG                                                                                   <  SRR4302537.3284925/94‑1 (MQ=60)
      TGACCTGATCACCTTTGTCGCTGACCGTCCGGGGCATGATCTGCGTTATGCCATTGATGCTTCGAAAATTGCCCGTGAACTTGGCTGGCTGCCG                                                                                   <  SRR4302537.2987694/94‑1 (MQ=60)
       GACCTGATCACCTTTGTCGCTGACCGTCCGGGGCATGATCTGCGTTATGCCATTGATGCTTCGAAAATTGCCCGTGAACTTGGCTGGCTGCCGC                                                                                  <  SRR4302537.2973328/94‑1 (MQ=60)
       GACCTGATCACCTTTGTCGCTGACCGTCCGGGGCATGATCTGCGTTATGCCATTGATGCTTCGAAAATTGCCCGTGAACTTGGCTGGCTGCCGC                                                                                  >  SRR4302537.2936603/1‑94 (MQ=60)
          CTGATCACCTTTGTCGCTGACCGTCCGGGGCATGATCTGCGTTATGCCATTGATGCTTCGAAAATTGCCCGTGAACTTGGCTGGCTGCCGCAGG                                                                               >  SRR4302537.1055390/1‑94 (MQ=60)
          CTGATCACCTTTGTCGCTGACCGTCCGGGGCATGATCTGCGTTATGCCATTGATGCTTCGAAAATTGCCCGTGAACTTGGCTGGCTGCCGCAGG                                                                               >  SRR4302537.2686000/1‑94 (MQ=60)
           TGATCACCTTTGTCGCTGACCGTCCGGGGCATGATCTGCGTTATGCCATTGATGCTTCGAAAATTGCCCGTGAACTTGGCTGGCTGCCGCAGGA                                                                              >  SRR4302537.2228159/1‑94 (MQ=60)
           TGATCACCTTTGTCGCTGACCGTCCGGGGCATGATCTGCGTTATGCCATTGATGCTTCGAAAATTGCCCGTGAACTTGGCTGGCTGCCGCAGGA                                                                              >  SRR4302537.4100173/1‑94 (MQ=60)
            GATCACCTTTGTCGCTGACCGTCCGGGGCATGATCTGCGTTATGCCATTGATGCTTCGAAAATTGCCCGTGAACTTGGCTGGCTGCCGTAGGAA                                                                             <  SRR4302537.3625358/94‑1 (MQ=60)
            GATCACCTTTGTCGCTGACCGTCCGGGGCATGATCTGCGTTATGCCATTGATGCTTCGAAAATTGCCCGTGAACTTGGCTGGCTGCCGCAGGAA                                                                             <  SRR4302537.5923610/94‑1 (MQ=60)
            GATCACCTTTGTCGCTGACCGTCCGGGGCATGATCTGCGTTATGCCATTGATGCTTCGAAAATTGCCCGTGAACTTGGCTGGCTGCCGCAGGAA                                                                             <  SRR4302537.5782046/94‑1 (MQ=60)
             ATCACCTTTGTCGCTTACCGTCCGGGGCATGATCTGCGTTATGTCATTGATGCTTCGAAAATTGCCCGTGAACTTGGCTGGCTGCCGCAGGAAA                                                                            >  SRR4302537.3480394/1‑94 (MQ=60)
               CACCTTTGTCGCTGACCGTCCGGGGCATGATCTGCGTTATGCCATTGATGCTTCGAAAATTGCCCGTGAACTTGGCTGGCTGCCGCAGGAAACC                                                                          <  SRR4302537.483175/94‑1 (MQ=60)
                ACCTTTGTCGCTGACCGTCCGGGGCATGATCTGCGTTATGCCATTGATGCTTCGAAAATTGCCCGTGAACTTGGCTGGCTGCCGCAGGAAACCT                                                                         >  SRR4302537.1278426/1‑94 (MQ=60)
                ACCTTTGTCGCTGACCGTCCGGGGCATGATCTGCGTTATGCCATTGATGCTTCGAAAATTGCCCGTGAACTTGGCTGGCTGCCGCAGGAAACCT                                                                         >  SRR4302537.2365201/1‑94 (MQ=60)
                ACCTTTGTCGCTGACCGTCCGGGGCATGATCTGCGTTATGCCATTGATGCTTCGAAAATTGCCCGTGAACTTGGCTGGCTGCCGCAGGAAACCT                                                                         >  SRR4302537.1932418/1‑94 (MQ=60)
                  CTTTGTCGCTGACCGTCCGGGGCATGATCTGCGTTATGCCATTGATGCTTCGAAAATTGCCCGTGAACTTGGCTGGCTGCCGCAGGAAACCTTT                                                                       <  SRR4302537.1084020/94‑1 (MQ=60)
                  CTTTGTCGCTGACCGTCCGGGGCATGATCTGCGTTATGCCATTGATGCTTCGAAAATTGCCCGTGAACTTGGCTGGCTGCCGCAGGAAACCTTT                                                                       >  SRR4302537.1557852/1‑94 (MQ=60)
                   TTTGTCGCTGACCGTCCGGGGCATGATCTGCGTTATGCCATTGATGCTTCGAAAATTGCCCGTGAACTTGGCTGGCTGCCGCAGGAAACCTTTG                                                                      >  SRR4302537.2713411/1‑94 (MQ=60)
                     TGTCGCTGACCGTCCGGGGCATGATCTGCGTTATGCCATTGATGCTTCGAAAATTGCCCGTGAACTTGGCTGGCTGCCGCAGGAAACCTTTGAA                                                                    <  SRR4302537.2120413/94‑1 (MQ=60)
                      GTCGCTGACCGTCCGGGGCATGATCTGCGTTATGCCATTGATGCTTCGAAAATTGCCCGTGAACTTGGCTGGCTGCCGCAGGAAACCTTTGAAA                                                                   <  SRR4302537.5497849/94‑1 (MQ=60)
                       TCGCTGACCGTCCGGGGCATGATCTGCGTTATGCCATTGATGCTTCGAAAATTGCCCGTGAACTTGGCTGGCTGCCGCAGGAAACCTTTGAAAG                                                                  <  SRR4302537.4415563/94‑1 (MQ=60)
                        CGCTGACCGTCCGGGGCATGATCTGCGTTATGCCATTGATGCTTCGAAAATTGCCCGTGAACTTGGCTGGCTGCCGCAGGAAACCTTTGAAAGT                                                                 <  SRR4302537.6444435/94‑1 (MQ=60)
                        CGCTGACCGTCCGGGGCATGATCTGCGTTATGCCATTGATGCTTCGAAAATTGCCCGTGAACTTGGCTGGCTGCCGCAGGAAACCTTTGAAAGG                                                                 >  SRR4302537.4170865/1‑94 (MQ=60)
                          CTGACCGTCCGGGGCATGATCTGCGTTATGCCATTGATGCTTCGAAAATTGCCCGTGAACTTGGCTGGCTGCCGCAGGAAACCTTTGAAAGTGG                                                               >  SRR4302537.5302392/1‑94 (MQ=60)
                           TGACCGTCCGGGGCATGATCTGCGTTATGCCATTGATGCTTCGAAAATTGCCCGTGAACTTGGCTGGCTGCCGCAGGAAACCTTTGAAAGTGGA                                                              >  SRR4302537.6088823/1‑94 (MQ=60)
                           TGACCGTCCGGGGCATGATCTGCGTTATGCCATTGATGCTTCGAAAATTGCCCGTGAACTTGGCTGGCTGCCGCAGGAAACCTTTGAAAGTGGA                                                              <  SRR4302537.519800/94‑1 (MQ=60)
                           TGACCGTCCGGGGCATGATCTGCGTTATGCCATTGATGCTTCGAAAATTGCCCGTGAACTTGGCTGGCTGCCGCAGGAAACCTTTGAAAGTGGA                                                              <  SRR4302537.5764150/94‑1 (MQ=60)
                           TGACCGTCCGGGGCATGATCTGCGTTATGCCATTGATGCTTCGAAAATTGCCCGTGAACTTGGCTGGCTGCCGCAGGAAACCTTTGAAAGTGGA                                                              >  SRR4302537.982916/1‑94 (MQ=60)
                           TGACCGTCCGGGGCATGATCTGCGTTATGCCATTGATGCTTCGAAAATTGCCCGTGAACTTGGCTGGCTGCCGCAGGAAACCTTTGAAAGTGGA                                                              <  SRR4302537.2714197/94‑1 (MQ=60)
                            GACCGTCCGGGGCATGATCTGCGTTATGCCATTGATGCTTCGAAAATTGCCCGTGAACTTGGCTGGCTGCCGCAGGAAACCTTTGAAAGTGGAA                                                             >  SRR4302537.5149151/1‑94 (MQ=60)
                            GACCGTCCGGGGCATGATCTGCGTTATGCCATTGATGCTTCGAAAATTGCCCGTGAACTTGGCTGGCTGCCGCAGGAAACCTTTGAAAGTGGAA                                                             <  SRR4302537.3523191/94‑1 (MQ=60)
                              CCGTCCGGGGCATGATCTGCGTTATGCCATTGATGCTTCGAAAATTGCCCGTGAACTTGGCTGGCTGCCGCAGGAAACCTTTGAAAGTGGAATG                                                           <  SRR4302537.5892722/94‑1 (MQ=60)
                              CCGTCCGGGGCATGATCTGCGTTATGCCATTGATGCTTCGAAAATTGCCCGTGAACTTGGCTGGCTGCCGCAGGAAACCTTTGAAAGTGGAATG                                                           <  SRR4302537.1394852/94‑1 (MQ=60)
                               CGTCCGGGGCATGATCTGCGTTATGCCATTGATGCTTCGAAAATTGCCCGTGAACTTGGCTGGCTGCCGCAGGAAACCTTTGAAAGTGGAATGC                                                          >  SRR4302537.188292/1‑94 (MQ=60)
                                GTCTGGGGAATGATCTGCGTTATGCCATTGATGCTTCGAAAATTGCCCGTGAACTTGGCTGGCTGCCGCAGGAAACCTTTGAAAGTGGAATGCG                                                         <  SRR4302537.3498864/94‑1 (MQ=60)
                                   CGGGGCATGATCTGCGTTATGCCATTGATGCTTCGAAAATTGCCCGTGAACTTGGCTGGCTGCCGCAGGAAACCTTTGAAAGTGGAATGCGTAA                                                      <  SRR4302537.2097632/94‑1 (MQ=60)
                                   CGGGGCATGATCTGCGTTATGCCATTGATGCTTCGAAAATTGCCCGTGAACTTGGCTGGCTGCCGCAGGAAACCTTTGAAAGTGGAATGCGTAA                                                      >  SRR4302537.1938974/1‑94 (MQ=60)
                                   CGGGGCATGATCTGCGTTATGCCATTGATGCTTCGAAAATTGCCCGTGAACTTGGCTGGCTGCCGCAGGAAACCTTTGAAAGTGGAATGCGTAA                                                      <  SRR4302537.2449153/94‑1 (MQ=60)
                                    GGGGCATGATCTGCGTTATGCCATTGATGCTTCGAAAATTGCCCGTGAACTTGGCTGGCTGCCGCAGGAAACCTTTGAAAGTGGAATGCGTAAA                                                     <  SRR4302537.4959700/94‑1 (MQ=60)
                                     GGGCATGATCTGCGTTATGCCATTGATGCTTCGAAAATTGCCCGTGAACTTGGCTGGCTGCCGCAGGAAACCTTTGAAAGTGGAATGCGTAAAA                                                    >  SRR4302537.2454047/1‑94 (MQ=60)
                                      GGCATGATCTGCGTTATGCCATTGATGCTTCGAAAATTGCCCGTGAACTTGGCTGGCTGCCGCAGGAAACCTTTGAAAGTGGAATGCGTAAAAC                                                   >  SRR4302537.5386056/1‑94 (MQ=60)
                                       GCATGATGTGCGTTATGCCATTGATGCTTCGAAAATTGCCCGTGAACTTGGCTGGCTGCCGCAGGAAACCTTTGAAAGTGGAATGCGTAAAACG                                                  <  SRR4302537.4265416/94‑1 (MQ=60)
                                       GCATGATCTGCGTTATGCCATTGATGCTTCGAAAATTGCCCGTGAACTTGGCTGGCTGCCGCAGGAAACCTTTGAAAGTGGAATGCGTAAAACG                                                  <  SRR4302537.3028047/94‑1 (MQ=60)
                                        CATGATCTGCGTTATGCCATTGATGCTTCGAAAATTGCCCGTGAACTTGGCTGGCTGCCGCAGGAAACCTTTGAAAGTGGAATGCGTAAAACGG                                                 >  SRR4302537.2753040/1‑94 (MQ=60)
                                         ATGATCTGCGTTATGCCATTGATGCTTCGAAAATTGCCCGTGAACTTGGCTGGCTGCCGCAGGAAACCTTTGAAAGTTGAATGCGTAAAACGGT                                                >  SRR4302537.5674046/1‑94 (MQ=60)
                                         ATGATCTGCGTTATGCCATTGATGCTTCGAAAATTGCCCGTGAACTTGGCTGGCTGCCGCAGGAAACCTTTGAAAGTGGAATGCGTAAAACGGT                                                >  SRR4302537.6382307/1‑94 (MQ=60)
                                         ATGATCTGCGTTATGCCATTGATGCTTCGAAAATTGCCCGTGAACTTGGCTGGCTGCCGCAGGAAACCTTTGAAAGTGGAATGCGTAAAACGGT                                                >  SRR4302537.4180152/1‑94 (MQ=60)
                                          TGATCTGCGTTATGCCATTGATGCTTCGAAAATTGCCCGTGAACTTGGCTGGCTGCCGCAGGAAACCTTTGAAAGTGGAATGCGTAAAACGGTT                                               <  SRR4302537.540715/94‑1 (MQ=60)
                                          TGATCTGCGTTATGCCATTGATGCTTCGAAAATTGCCCGTGAACTTGGCTGGCTGCCGCAGGAAACCTTTGAAAGTGGAATGCGTAAAACGGTT                                               >  SRR4302537.2853009/1‑94 (MQ=60)
                                           GATCTGCGTTATGCCATTGATGCTTCGAAAATTGCCCGTGAACTTGGCTGGCTGCCGCAGGAAACCTTTGAAAGTGGAATGCGTAAAACGGTTC                                              >  SRR4302537.817101/1‑94 (MQ=60)
                                            ATCTGCGTTATGCCATTGATGCTTCGAAAATTGCCCGTGAACTTGGCTGGCTGCCGCAGGAAACCTTTGAAAGTGGAATGCGTAAAACGGTTCA                                             >  SRR4302537.1232241/1‑94 (MQ=60)
                                            ATCTGCGTTATGCCATTGATGCTTCGAAAATTGCCCGTGAACTTGGCTGGCTGCCGCAGGAAACCTTTGAAAGTGGAATGCGTAAAACGGTTCA                                             <  SRR4302537.1532656/94‑1 (MQ=60)
                                            ATCTGCGTTATGCCATTGATGCTTCGAAAATTGCCCGTGAACTTGGCTGGCTGCCGCAGGAAACCTTTGAAAGTGGAATGCGTAAAACGGTTCA                                             >  SRR4302537.4007558/1‑94 (MQ=60)
                                               TGCGTTATGCCATTGATGCTTCGAAAATTGCCCGTGAACTTGGCTGGCTGCCGCAGGAAACCTTTGAAAGTGGAATGCGTAAAACGGTTCAGTG                                          <  SRR4302537.3138458/94‑1 (MQ=60)
                                               TGCGTTATGCCATTGATGCTTCGAAAATTGCCCGTGAACTTGGCTGGCTGCCGCAGGAAACCTTTGAAAGTGGAATGCGTAAAACGGTTCAGTG                                          >  SRR4302537.4249512/1‑94 (MQ=60)
                                                GCGTTATGCCATAGATGCTTCGAAAATTGCCCGTGAACGTGGCTGGCTGCCGCAGGAAACCTTTGAAAGTGGAATGCGTAAAACGGTTCAGTGG                                         <  SRR4302537.207773/94‑1 (MQ=60)
                                                 CGTTATGCCATTGATGCTTCGAAAATTGCCCGTGAACTTGGCTGGCTGCCGCAGGAAACCTTTGAAAGTGGAATGCGTAAAACGGTTCAGTGGT                                        <  SRR4302537.4818219/94‑1 (MQ=60)
                                                 CGTTATGCCATTGATGCTTCGAAAAGTGCCCGTGAACTTGGCTGGCTGCCGCAGGAAACCTTTGAAAGTGGAATGCGTAAAACGGTTCAGTGGT                                        <  SRR4302537.6050967/94‑1 (MQ=60)
                                                  GTTATGCCATTGATGCTTCGAAAATTGCCCGTGAACTTGGCTGGCTGCCGCAGGAAACCTTTGAAAGTGGAATGCGTAAAACGGTTCAGTGGTA                                       >  SRR4302537.903694/1‑94 (MQ=60)
                                                  GTTATGCCATTGATGCTTCGAAAATTGCCCGTGAACTTGGCTGGCTGCCGCAGGAAACCTTTGAAAGTGGAATGCGTAAAACGGTTCAGTGGTA                                       >  SRR4302537.873127/1‑94 (MQ=60)
                                                   TTATGCCATTGATGCTTCGAAAATTGCCCGTGAACTTGGCTGGCTGCCGCAGGAAACCTTTGAAAGTGGAATGCGTAAAACGGTTCAGTGGTAT                                      >  SRR4302537.2083332/1‑94 (MQ=60)
                                                   TTATGCCATTGATGCTTCGAAAATTGCCCGTGAACTTGGCTGGCTGCCGCAGGAAACCTTTGAAAAAGGAATGCGTAAAACGGTTCAGTGGTAT                                      >  SRR4302537.4875963/1‑94 (MQ=60)
                                                    TATGCCATTGATGCTTCGAAAATTGCCCGTGAACTTGGCTGGCTGCCGCAGGAAACCTTTGAAAGTGGAATGCGTAAAACGGTTCAGTGGTATC                                     <  SRR4302537.1768347/94‑1 (MQ=60)
                                                     ATGCCATTGATGCTTCGAAAATTGCCCGTGAACTTGGCTGGCTGCCGCAGGAAACCTTTGAAAGTGGAATGCGTAAAACGGTTCAGTGGTATCT                                    >  SRR4302537.2048090/1‑94 (MQ=60)
                                                      TGCCATTGATGCTTCGAAAATTGCCCGTGAACTTGGCTGGCTGCCGCAGGAAACCTTTGAAAGTGGAATGCGTAAAACGGTTCAGTGGTATCTG                                   <  SRR4302537.1989289/94‑1 (MQ=60)
                                                       GCCATTGATGCTTCGAAAATTGCCCGTGAACTTGGCTGGCTGCCGCAGGAAACCTTTGAAAGTGGAATGCGTAAAACGGTTCAGTGGTATCTGG                                  <  SRR4302537.3887684/94‑1 (MQ=60)
                                                        CCATTGATGCTTCGAAAATTGCCCGTGAACTTGGCTGGCTGCCGCAGGAAACCTTTGAAAGTGGAATGCGTAAAACGGTTCAGTGGTATCTGGC                                 >  SRR4302537.4024024/1‑94 (MQ=60)
                                                         CATTGATGCTTCGAAAATTGCCCGTGAACTTGGCTGGCTGCCGCAGGAAACCTTTGAAAGTGGAATGCGTAAAACGGTTCAGTGGTATCTGGCT                                >  SRR4302537.767629/1‑94 (MQ=60)
                                                         CATTGATGCTTCGAAAATTGCCCGTGAACTTGGCTGGCTGCCGCAGGAAACCTTTGAAAGGGGCATGCGGAAAACGGTTCAGTGGTATCTGGCT                                >  SRR4302537.1731094/1‑94 (MQ=60)
                                                         CATTGATGCTTCGAAAATTGCCCGTGAACTTGGATGGCTGCCGCAGGAAACCTTTGAAAGTGGAATGCGTAAAACGGTTCAGTGGTATCTGGCT                                >  SRR4302537.3099212/1‑94 (MQ=60)
                                                          ATTGATGCTTCGAAAATTGCCCGTGAACTTGGCTGGCTGCCGCAGGAAACCTTTGAAAGTGGAATGCGTAAAACGGTTCAGTGGTATCTGGCTA                               >  SRR4302537.774064/1‑94 (MQ=60)
                                                          ATTGATGCTTCGAAAATTGCCCGTGAACTTGGCTGGCTGCCGCAGGAAACCTTTGAAAGTGGAATGCGTAAAACGGTTCAGTGGTATCTGGCTA                               <  SRR4302537.2501775/94‑1 (MQ=60)
                                                           TTGATGCTTCGAAAATTGCCCGTGAACTTGGCTGGCTGCCGCAGGAAACCTTTGAAAGTGGAATGCGTAAAACGGTTCAGTGGTATCTGGCTAA                              >  SRR4302537.5171321/1‑94 (MQ=60)
                                                            TGATGCTTCGAAAATTGCCCGTGAACTTGGCTGGCTGCCGCAGGAAACCTTTGAAAGTGGAATGCGTAAAACGGTTCAGTGGTATCTGGCTAAT                             <  SRR4302537.3675936/94‑1 (MQ=60)
                                                            TGATGCTTCGAAAATTGCCCGTGAACTTGGCTGGCTGCCGCAGGAAACCTTTGAAAGTGGAATGCGTAAAACGGTTCAGGGGTATCTGGCTAAT                             >  SRR4302537.5328373/1‑94 (MQ=60)
                                                             GATGCTTCGAAAATTGCCCGTGAACTTGGCTGGCTGCCGCAGGAAACCTTTGAAAGTGGAATGCGTAAAACGGTTCAGTGGTATCTGGCTAATG                            >  SRR4302537.1521017/1‑94 (MQ=60)
                                                              ATGCTTCGAAAATTGCCCGTGAACTTGGCTGGCTGCCGCAGGAAACCTTTGAAAGTGGAATGCGTAAAACGGTTCAGTGGTATCTGGCTAATGA                           >  SRR4302537.640149/1‑94 (MQ=60)
                                                               TGCTTCGAAAATTGCCCGTGAACTTGGCTGGCTGCCGCAGGAAACCTTTGAAAGTGGAATGCGTAAAACGGTTCAGTGGTATCTGGCTAATGAA                          >  SRR4302537.5621255/1‑94 (MQ=60)
                                                                GCTTCGAAAATTGCCCGTGAACTTGGCTGGCTGCCGCAGGAAACCTTTGAAAGTGGAATGCGTAAAACGGTTCAGTGGTATCTGGCTAATGAAA                         >  SRR4302537.1258702/1‑94 (MQ=60)
                                                                 CTTCGAAAATTGCCCGTGAACTTGGCTGGCTGCCGCAGGAAACCTTTGAAAGTGGAATGCGTAAAACGGTTCAGTGGTATCTGGCTAATGAAAG                        >  SRR4302537.3057736/1‑94 (MQ=60)
                                                                 CTTCGAAAATTGCCCGTGAACTTCGCTGGCTGCCGCAGGAAACCTTTGTAAGTGGAATGCGTCAAACGCTTCAGTGGTATCTGGCTAATAAAAG                        >  SRR4302537.4011371/1‑94 (MQ=60)
                                                                  TTCGAAAATTGCCCGTGAACTTGGCTGGCTGCCGCAGGAAACCTTTGAAAGTGGAATGCGTAAAACGGTTCAGTGGTATCTGGCTAATGAAAGC                       >  SRR4302537.384885/1‑94 (MQ=60)
                                                                  TTCGAAAATCGCCCGTGAACTTGGCTGGCTGCCGCAGGAAACCTTTGAAAGTGGAATGCGTAAAACGGTTCAGTGGTATCTGGCTAATGAAAGC                       >  SRR4302537.2359326/1‑94 (MQ=60)
                                                                   TCGAAAATTGCCCGTGAACTTGGCTGGCTGCCGCAGGAAACCTTTGAAAGTGGAATGCGTAAAACGGTTCAGTGGTATCTGGCTAATGAAAGCT                      >  SRR4302537.3860593/1‑94 (MQ=60)
                                                                   TCGAAAATTGCCCGTGAACTTGGCTGGCTGCCGCAGGAAACCTTTGAAAGTGGAATGCGTAAAACGGTTCAGTGGTATCTGGCTAATGAAAGCT                      >  SRR4302537.6193521/1‑94 (MQ=60)
                                                                    CGAAAATTGCCCGTGAACTTGGCTGGCTGCCGCAGGAAACCTTTGAAAGTGGAATGCGTAAAACGGTTCAGTGGTATCTGGCTAATGAAAGCTG                     >  SRR4302537.2352778/1‑94 (MQ=60)
                                                                    CGAAAATTGCCCGTGAACTTGGCTGGCTGCCGCAGGAAACCTTTGAAAGTGGAATGCGTAAAACGGTTCAGTGGTATCTGGCTAATGAAAGCTG                     >  SRR4302537.5101348/1‑94 (MQ=60)
                                                                     GAAAATTGCCCGTGAACTTGGCTGGCTGCCGCAGGAAACCTTTGAAAGTGGAATGCGTAAAACGGTTCAGTGGTATCTGGCTAATGAAAGCTGG                    >  SRR4302537.4311845/1‑94 (MQ=60)
                                                                     GAAAATTGCCCGTGAACTTGGCTGGCTGCCGCAGGAAACCTTTGAAAGTGGAATGCGTAAAACGGTTCAGTGGTATCTGGCTAATGAAAGCTGG                    <  SRR4302537.3124308/94‑1 (MQ=60)
                                                                      AAAATTGCCCGTGAACTTGGCTGGCTGCCGCAGGAAACCTTTGAAAGTGGAATGCGTAAAACGGTTCAGTGGTATCTGGCTAATGAAAGCTGGT                   <  SRR4302537.6284411/94‑1 (MQ=60)
                                                                       AAATTGCCCGTGAACTTGGCTGGCTGCCGCAGGAAACCTTTGAAAGTGGAATGCGTAAAACGGTTCAGTGGTATCTGGCTAATGAAAGCTGGTG                  >  SRR4302537.5781105/1‑94 (MQ=60)
                                                                        AATTGCCCGTGAACTTGGCTGGCTGCCGCAGGAAACCTTTGAAAGTGGAATGCGTAAAACGGTTCAGTGGTATCTGGCTAATGAAAGCTGGTGG                 >  SRR4302537.5706250/1‑94 (MQ=60)
                                                                         ATTGCCCGTGAACTTGGCTGGCTGCCGCAGGAAACCTTTGAAAGTGGAATGCGTAAAACGGTTCAGTGGTATCTGGCTAATGAAAGCTGGTGGA                >  SRR4302537.2990965/1‑94 (MQ=60)
                                                                         ATTGCCCGTGAACTTGGCTGGCTGCCGCAGGAAACCTTTGAAAGTGGAATGCGTAAAACGGTTCAGTGGTATCTGGCTAATGAAAGCTGGTGGA                >  SRR4302537.3719060/1‑94 (MQ=60)
                                                                           TGCCCGTGAACTTGGCTGGCTGCCGCAGGAAACCTTTGAAAGTGGAATGCGTAAAACGGTTCAGTGGTATCTGGCTAATGAAAGCTGGTGGAAG              <  SRR4302537.3703484/94‑1 (MQ=60)
                                                                            GCCCGTGAACTTGGCTGGCTGCCGCAGGAAACCTTTGAAAGTGGAATGCGTAAAACGGTTCAGTGGTATCTGGCTAATGAAAGCTGGTGGAAGC             >  SRR4302537.608742/1‑94 (MQ=60)
                                                                             CCCGTGAACTTGGCTGGCTGCCGCAGGAAACCTTTGAAAGTGGAATGCGTAAAACGGTTCAGTGGTATCTGGCTAATGAAAGCTGGTGGAAGCA            <  SRR4302537.5372547/94‑1 (MQ=60)
                                                                              CCGTGAACTTGGCTGGCTGCCGCAGGAAACCTTTGAAAGTGGAATGCGTAAAACGGTTCAGTGGTATCTGGCTAATGAAAGCTGGTGGAAGCAG           >  SRR4302537.999610/1‑94 (MQ=60)
                                                                              CCGTGAACTTGGCTGGCTGCCGCAGGAAACCTTTGAAAGTGGAATGCGTAAAACGGTTCAGTGGTATCTGGCTAATGAAAGCTGGTGGAAGCAG           >  SRR4302537.5880956/1‑94 (MQ=60)
                                                                              CCGTGAACTTGGCTGGCTGCCGCAGGAAACCTTTGAAAGTGGAATGCGTAAAACGGTTCAGTGGTATCTGGCTAATGAAAGCTGGTGGAAGCAG           <  SRR4302537.1072220/94‑1 (MQ=60)
                                                                              CCGTGAACTTGGCTGGCTGCCGCAGGAAACCTTTGAAAGTGGAATGCGTAAAACGGTTCAGTGGTATCTGGCTAATGAAAGCTGGTGGAAGCAG           <  SRR4302537.1141027/94‑1 (MQ=60)
                                                                               CGTGAACTTGGCTGGCTGCCGCAGGAAACCTTTGAAAGTGGAATGCGTAAAACGGTTCAGTGGTATCTGGCTAATGAAAGCTGGTGGAAGCAGG          <  SRR4302537.4764599/94‑1 (MQ=60)
                                                                               CGTGAACTTGGCTGGCTGCCGCAGGAAACCTTTGAAAGTGGAATGCGTAAAACGGTTCAGTGGTATCTGGCTAATGAAAGCTGGTGGAAGCAGG          <  SRR4302537.2719391/94‑1 (MQ=60)
                                                                               CGTGAACTTGGCTGGCTGCCGCAGGAAACCTTTGAAAGTGGAATGCGTAAAACGGTTCAGTGGTATCTGGCTAATGAAAGCTGGTGGAAGCAGG          >  SRR4302537.3612429/1‑94 (MQ=60)
                                                                                GTGAACTTGGCTGGCTGCCGCAGGAAACCTTTGAAAGTGGAATGCGTAAAACGGTTCAGTGGTATCTGGCTAATGAAAGCTGGTGGAAGCAGGT         >  SRR4302537.488412/1‑94 (MQ=60)
                                                                                GTGAACTTGGCTGGCTGCCGCAGGAAACCTTTGAAAGTGGAATGCGTAAAACGGTTCAGTGGTATCTGGCTAATGAAAGCTGGTGGAAGCAGGT         <  SRR4302537.2637069/94‑1 (MQ=60)
                                                                                GTGAACTTGGCTGGCTGCCGCAGGAAACCTTTGAAAGTGGAATGCGTAAAACGGTTCAGTGGTATCTGGCTAATGAAAGCTGGTGGAAGCAGGG         >  SRR4302537.4354303/1‑94 (MQ=60)
                                                                                GTGAACTTGGCTGGCTGCCGCAGGAAACCTTTGAAAGTGGAATGCGTAAAACGGTTCAGTGGTATCTGGCTAATGAAAGCTGGTGGAAGCAGGG         >  SRR4302537.4354305/1‑94 (MQ=60)
                                                                                 TGAACTTGGCTGGCTGCCGCAGGAAACCTTTGAAAGTGGAATGCGTAAAACGGTTCAGTGGTATCTGGCTAATCAAAGCTGGTGGAAGCAGGTG        >  SRR4302537.4334259/1‑94 (MQ=60)
                                                                                  GAACTTGGCTGGCTGCCGCAGGAAACCTTTGAAAGTGGAATGCGTAAAACGGTTCAGTGGTATCTGGCTAATGAAAGCTGGTGGAAGCAGGTGC       <  SRR4302537.3337517/94‑1 (MQ=60)
                                                                                   AACTTGGCTGGCTGCCGCAGGAAACCTTTGAAAGTGGAATGCGTAAAACGGTTCAGTGGTATCTGGCTAATGAAAGCTGGTGGAAGCAGGggcg      >  SRR4302537.1302512/1‑90 (MQ=60)
                                                                                     CTTGGCTGGCTGCCGCAGGAAACCTTTGAAAGTGGAATGCGTAAAACGGTTCAGTGGTATCTGGCTAATGAAAGCTGGTGGAAGCAGGTGCAGG    <  SRR4302537.2314868/94‑1 (MQ=60)
                                                                                      TTGGCTGGCTGCCGCAGGAAACCTTTGAAAGTGGAATGCGTAAAACGGTTCAGTGGTATCTGGCTAATGAAAGCTGGTGGAAGCAGGTGCAGGA   >  SRR4302537.1230174/1‑94 (MQ=60)
                                                                                       TGGCTGGCTGCCGCAGGAAACCTTTGAAAGTGGAATGCGTAAAACGGTTCAGTGGTATCTGGCTAATGAAAGCTGGTGGAAGCAGGTGCAGGAC  <  SRR4302537.2873782/94‑1 (MQ=60)
                                                                                       TGGCTGGCTGCCGCAGGAAACCTTTGAAAGTGGAATGCGTAAAACGGTTCAGTGGTATCTGGCTAATGAAAGCTGGTGGAAGCAGGTGCAGGAC  >  SRR4302537.6240280/1‑94 (MQ=60)
                                                                                             |                                                                                       
CTATCGTGACCTGATCACCTTTGTCGCTGACCGTCCGGGGCATGATCTGCGTTATGCCATTGATGCTTCGAAAATTGCCCGTGAACTTGGCTGCGTGCCGCAGGAAACCTTTGAAAGTGGAATGCGTAAAACGGTTCAGTGGTATCTGGCTAATGAAAGCTGGTGGAAGCAGGTGCAGGAC  >  NC_000913/3970982‑3971162

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 7 ≤ ATCG/ATCG < 28 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑