Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A1 F1 I2 R1	882	254.4	12957588	98.0%	12698436	93.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,972,637	2 bp→GC	coding (430‑431/546 nt)	wecD →	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,972,637	0	C	G	100.0%	659.1 / NA	188	R144A (CGT→GCT)	wecD	hypothetical protein
Reads supporting (aligned to +/- strand): ref base C (0/0); new base G (79/109); total (79/109)
*	NC_000913	3,972,638	0	G	C	99.5%	656.1 / NA	188	R144A (CGT→GCT)	wecD	hypothetical protein
Reads supporting (aligned to +/- strand): ref base G (0/0); major base C (78/109); minor base A (1/0); total (79/109)
Fisher's exact test for biased strand distribution p-value = 4.20e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 191 total aligned reads displayed.

AATGCGACAGATGCGCGAATTGGCCTGCTGGCTGGACGCGGTGCAGGTGCTGAGCTGATGCAAACGGCGCTAAACTGGGCGTATCGTCGCGGTAAAACAACTTTGCGGGTGGCGACCCAAATGGGCAACACCGCCGCGCTTAAACGATACATACAAAGTGGTGCGAATGTAG  >  NC_000913/3972553‑3972724

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aaTGCGACAGATGCGCGAATTGGCCTGCTGGCTGGACGCGGTGCAGGTGCTGAGCTGATGCAAACGGCGCTAAACTGGGCGTATGCTCGCgga                                                                                 >  1:498314/1‑92 (MQ=255)

aaTGCGACAGATGCGCGAATTGGCCTGCTGGCTGGACGCGGTGCAGGTGCTGAGCTGATGCAAACGGCGCTAAACTGGGCGTATGCTCGCGGt                                                                                 >  2:510620/1‑93 (MQ=255)

 aTGCGACAGATGCGCGAATTGGCCTGCTGGCTGGACGCGGTGCAGGTGCTGAGCTGATGCAAACGGCGCTAAACTGGGCGTATGCTCGCGGTa                                                                                >  1:2552138/1‑93 (MQ=255)

 aTGCGACAGATGCGCGAATTGGCCTGCTGGCTGGACGCGGTGCAGGTGCTGAGCTGATGCAAACGGCGCTAAACTGGGCGTATGCTCGCGGTa                                                                                >  2:5602112/1‑93 (MQ=255)

  tGCGACAGATGCGCGAATTGGCCTGCTGGCTGGACGCGGTGCAGGTGCTGAGCTGATGCAAACGGCGCTAAACTGGGCGTATGCTCGCGGTaa                                                                               <  1:5629976/93‑1 (MQ=255)

     gACAGATGCGCGAATTGGCCTGCTGGCTGGACGCGGTGCAGGTGCTGAGCTGATGCAAACGGCGCTAAACTGGGCGTATGCTCGCGGTAAaac                                                                            >  2:6016595/1‑93 (MQ=255)

      aCAGATGCGCGAATTGGCCTGCTGGCTGGACGCGGTGCAGGTGCTGAGCTGATGCAAACGGCGCTAAACTGGGCGTATGCTCGCGGTAAaaca                                                                           >  2:2104043/1‑93 (MQ=255)

       cAGATGCGCGAATTGGCCTGCTGGCTGGACGCGGTGCAGGTGCTGAGCTGATGCAAACGGCGCTAAACTGGGCGTATGCTCGCGGTAAaacaa                                                                          <  2:1194782/93‑1 (MQ=255)

         gATGCGCGAATTGGCCTGCTGGCTGGACGCGGTGCAGGTGCTGAGCTGATGCAAACGGCGCTAAACTGGGCGTATGCTCGCGGTAAAACAACt                                                                        <  1:529789/93‑1 (MQ=255)

         gATGCGCGAATTGGCCTGCTGGCTGGACGCGGTGCAGGTGCTGAGCTGATGCAAACGGCGCTAAACTGGGCGTATGCTCGCGGTAAAACAACt                                                                        <  1:2961160/93‑1 (MQ=255)

          aTGCGCGAATTGGCCTGCTGGCTGGACGCGGTGCAGGTGCTGAGCTGATGCAAACGGCGCTAAACTGGGCGTATGCTCGCGGTAAAACAACtt                                                                       <  1:3867662/93‑1 (MQ=255)

          aTGCGCGAATTGGCCTGCTGGCTGGACGCGGTGCAGGTGCTGAGCTGATGCAAACGGCGCTAAACTGGGCGTATGCTCGCGGTAAAACAACtt                                                                       <  1:3312633/93‑1 (MQ=255)

           tGCGCGAATTGGCCTGCTGGCTGGACGCGGTGCAGGTGCTGAGCTGATGCAAACGGCGCTAAACTGGGCGTATGCTCGCGGTAAAACAACttt                                                                      <  2:2069930/93‑1 (MQ=255)

           tGCGCGAATTGGCCTGCTGGCTGGACGCGGTGCAGGTGCTGAGCTGATGCAAACGGCGCTAAACTGGGCGTATGCTCGCGGTAAAACAACttt                                                                      >  2:3437530/1‑93 (MQ=255)

           tGCGCGAATTGGCCTGCTGGCTGGACGCGGTGCAGGTGCTGAGCTGATGCAAACGGCGCTAAACTGGGCGTATGATCGCGGTAAAACAACttt                                                                      >  2:3115622/1‑93 (MQ=255)

             cgcgAATTGGCCTGCTGGCTGGACGCGGTGCAGGTGCTGAGCTGATGCAAACGGCGCTAAACTGGGCGTATGCTCGCGGTAAAACAACTTTGc                                                                    >  1:6223921/1‑93 (MQ=255)

              gcgAATTGGCCTGCTGGCTGGACGCGGTGCAGGTGCTGAGCTGATGCAAACGGCGCTAAACTGGGCGTATGCTCGCGGTAAAACAACTTTGCg                                                                   >  1:2893465/1‑93 (MQ=255)

                gAATTGGCCTGCTGGCTGGACGCGGTGCAGGTGCTGAGCTGATGCAAACGGCGCTAAACTGGGCGTATGCTCGCGGTAAAACAACTTTGCggg                                                                 <  1:5417365/93‑1 (MQ=255)

                gAATTGGCCTGCTGGCTGGACGCGGTGCAGGTGCTGAGCTGATGCAAACGGCGCTAAACTGGGCGTATGCTCGCGGTAAAACAACTTTGCggg                                                                 >  1:4434597/1‑93 (MQ=255)

                gAATTGGCCTGCTGGCTGGACGCGGTGCAGGTGCTGAGCTGATGCAAACGGCGCTAAACTGGGCGTATGCTCGCGGTAAAACAACTTTGCggg                                                                 >  2:1173217/1‑93 (MQ=255)

                 aaTTGGCCTGCTGGCTGGACGCGGTGCAGGTGCTGAGCTGATGCAAACGGCGCTAAACTGGGCGTATGCTCGCGGTAAAACAACTTTGCGGGt                                                                >  2:4414383/1‑93 (MQ=255)

                 aaTTGGCCTGCTGGCTGGACGCGGTGCAGGTGCTGAGCTGATGCAAACGGCGCTAAACTGGGCGTATGCTCGCGGTAAAACAACTTTGCGGGt                                                                <  1:4307503/93‑1 (MQ=255)

                 aaTTGGCCTGCTGGCTGGACGCGGTGCAGGTGCTGAGCTGATGCAAACGGCGCTAAACTGGGCGTATGCTCGCGGTAAAACAACTTTGCGGGt                                                                <  1:638351/93‑1 (MQ=255)

                 aaTTGGCCTGCTGGCTGGACGCGGTGCAGGTGCTGAGCTGATGCAAACGGCGCTAAACTGGGCGTATGCTCGCGGTAAAACAACTTTGCGGGt                                                                <  2:4314803/93‑1 (MQ=255)

                  aTTGGCCTGCTGGCTGGACGCGGTGCAGGTGCTGAGCTGATGCAAACGGCGCTAAACTGGGCGTATGCTCGCGGTAAAACAACTTTGCGGGTg                                                               >  1:4240529/1‑93 (MQ=255)

                  aTTGGCCTGCTGGCTGGACGCGGTGCAGGTGCTGAGCTGATGCAAACGGCGCTAAACTGGGCGTATGCTCGCGGTAAAACAACTTTGCGGGTg                                                               >  1:1144842/1‑93 (MQ=255)

                      gCCTGCTGGCTGGACGCGGTGCAGGTGCTGAGCTGATGCCAACGGCGCTAAACTGGGCGTATGCTCGCGGTAAAACAACTTTGCGGGTGGCGa                                                           <  1:5003129/93‑1 (MQ=255)

                      gCCTGCTGGCTGGACGCGGTGCAGGTGCTGAGCTGATGCAAACGGCGCTAAACTGGGCGTATGCTCGCGGTAAAACAACTTTGCGGGTGGCGa                                                           >  1:2064527/1‑93 (MQ=255)

                      gCCTGCTGGCTGGACGCGGTGCAGGTGCTGAGCTGATGCAAACGGCGCTAAACTGGGCGTATGCTCGCGGTAAAACAACTTTGCGGGTGGCGa                                                           <  1:5003130/93‑1 (MQ=255)

                        ctgctgGCTGGACGCGGTGCAGGTGCTGAGCTGATGCAAACGGCGCTAAACTGGGCGTATGCTCGCGGTAAAACAACTTTGCGGGTGGCGAcc                                                         >  2:4023291/1‑93 (MQ=255)

                        ctgctgGCTGGACGCGGTGCAGGTGCTGAGCTGATGCAAACGGCGCTAAACTGGGCGTATGCTCGCGGTAAAACAACTTTGCGGGTGGCGAcc                                                         >  2:3694731/1‑93 (MQ=255)

                         tgctgGCTGGACGCGGTGCAGGTGCTGAGCTGATGCAAACGGCGCTAAACTGGGCGTATGCTCGCGGTAAAACAACTTTGCGGGTGGCGAccc                                                        <  2:4975168/93‑1 (MQ=255)

                            tggctggACGCGGTGCAGGTGCTGAGCTGATGCAAACGGCGCTAAACTGGGCGTATGCTCGCGGTAAAACAACTTTGCGGGTGGCGACCCaaa                                                     >  2:5731190/1‑93 (MQ=255)

                            tggctggACGCGGTGCAGGTGCTGAGCTGATGCAAACGGCGCTAAACTGGGCGTATGCTCGCGGTAAAACAACTTTGCGGGTGGCGACCCaaa                                                     <  2:6282629/93‑1 (MQ=255)

                            tggctggACGCGGTGCAGGTGCTGAGCTGATGCAAACGGCGCTAAACTGGGCGTATGCTCGCGGTAAAACAACTTTGCGGGTGGCGACCCaaa                                                     <  2:1653735/93‑1 (MQ=255)

                             ggctggACGCGGTGCAGGTGCTGAGCTGATGCAAACGGCGCTAAACTGGGCGTATGCTCGCGGTAAAACAACTTTGCGGGTGGCGACCCAAAt                                                    <  1:3147515/93‑1 (MQ=255)

                             ggctggACGCGGTGCAGGTGCTGAGCTGATGCAAACGGCGCTAAACTGGGCGTATGCTCGCGGTAAAACAACTTTGCGGGTGGCGACCCAAAt                                                    <  1:3554297/93‑1 (MQ=255)

                                tggACGCGGTGCAGGTGCTGAGCTGATGCAAACGGCGCTAAACTGGGCGTATGCTCGCGGTAAAACAACTTTGCGGGTGGCGACCCAAATggg                                                 <  2:6103611/93‑1 (MQ=255)

                                 ggACGCGGTGCAGGTGCTGAGCTGATGCAAACGGCGCTAAACTGGGCGTATGCTCGCGGTAAAACAACTTTGCGGGTGGCGACCCAAATGGGc                                                <  1:5862177/93‑1 (MQ=255)

                                 ggACGCGGGGCAGGTGCTGAGCTGATGCAAACGGCGCTAAACTGGGCGTATGCTCGCGGTCAAACAACTTTGCGGGTGGCGACCCAAATGGGc                                                >  2:1282772/1‑93 (MQ=255)

                                  gACGCGGTGCAGGTGCTGAGCTGATGCAAACGGCGCTAAACTGGGCGTATGCTCGCGGTAAAACAACTTTGCGGGTGGCGACCCAAATGGGCa                                               <  2:3265904/93‑1 (MQ=255)

                                   aCGCGGTGCAGGTGCTGAGCTGATGCAAACGGCGCTAAACTGGGCGTATGCTCGTGGTAAAACAACTTTGCGGGTGGCGACCCAAATGGGCaa                                              >  1:5075582/1‑93 (MQ=255)

                                    cgcgGTGCAGGTGCTGAGCTGATGCAAACGGCGCTAAACTGGGCGTATGCTCGCGGTAAAACAACTTTGCGGGTGGCGACCCAAATGGGCAac                                             <  2:3950577/93‑1 (MQ=255)

                                      cgGTGCAGGTGCTGAGCTGATGCAAACGGCGCTAAACTGGGCGTATGCTCGCGGTAAAACAACTTTGCGGGTGGCGACCCAAATGGGCAacac                                           <  1:571442/93‑1 (MQ=255)

                                       ggTGCAGGTGCTGAGCTGATGCAAACGGCGCTAAACTGGGCGTATGCTCGCGGTAAAACAACTTTGCGGGTGGCGACCCAAATGGGCAACAcc                                          <  2:2671698/93‑1 (MQ=255)

                                          gCAGGTGCTGAGCTGATGCAAACGGCGCTAAACTGGGCGTATGCTCGCGGTAAAACAACTTTGCGGGTGGCGACCCAAATGGGCAACAccgcc                                       <  1:2567753/93‑1 (MQ=255)

                                           cAGGTGCTGAGCTGATGCAAACGGCGCTAAACTGGGGGTATGCTCGCGGTAAAACAACTTTGCGGGTGGCGACCCAAATGGGCAACAccgccg                                      >  2:2664227/1‑93 (MQ=255)

                                           cAGGTGCTGAGCTGATGCAAACGGCGCTAAACTGGGCGTATGCTCGCGGTAAAACAACTTTGCGGGTGGCGACCCAAATGGGCAACAccgccg                                      >  2:5363388/1‑93 (MQ=255)

                                            aGGTGCTGAGCTGATGCAAACGGCGCTAAACTGGGCGTATGCTCGCGGTAAAACAACTTTGCGGGTGGCGACCCAAATGGGCAACACcgccgc                                     <  2:4645336/93‑1 (MQ=255)

                                             ggTGCTGAGCTGATGCAAACGGCGCTAAACTGGGCGTATGCTCGCGGTAAAACAACTTTGCGGGTGGCGACCCAAATGGGCAACACCGCcgcg                                    >  1:1521627/1‑93 (MQ=255)

                                              gTGCTGAGCTGATGCAAACGGCGCTAAACTGGGCGTATGCTCGCGGTAAAACAACTTTGCGGGTGGCGACCCAAATGGGCAACACCGCCgcgc                                   >  1:886538/1‑93 (MQ=255)

                                              gTGCTGAGCTGATGCAAACGGCGCTAAACTGGGCGTATGCTCGCGGTAAAACAACTTTGCGGGTGGCGACCCAAATGGGCAACACCGCCgcgc                                   <  1:4873360/93‑1 (MQ=255)

                                               tGCTGAGCTGATGCAAACGGCGCTAAACTGGGCGTATGCTCGCGGTAAAACAACTTTGCGGGTGGCGACCCAAATGGGCAACACCGCCGCGCt                                  >  2:3986160/1‑93 (MQ=255)

                                               tGCTGAGCTGATGCAAACGGCGCTAAACTGGGCGTATGCTCGCGGTAAAACAACTTTGCGGGTGGCGACCCAAATGGGCAACACCGCCGCGCt                                  >  1:3692474/1‑93 (MQ=255)

                                                gctgagctgaTGCAAACGGCGCTAAACTGGGCGTATGCTCGCGGTAAAACAACTTTGCGGGTGGCGACCCAAATGGGCAACACCGCCGCGCtt                                 >  2:3960384/1‑93 (MQ=255)

                                                gctgagctgaTGCAAACGGCGCTAAACTGGGCGTATGCTCGCGGTAAAACAACTTTGCGGGTGGCGACCCAAATGGGCAACACCGCCGCGCtt                                 <  2:1434143/93‑1 (MQ=255)

                                                gctgagctgaTGCAAACGGCGCTAAACTGGGCGTATGCTCGCGGTAAAACAACTTTGCGGGTGGCGACCCAAATGGGCAACACCGCCGCGCtt                                 >  1:2887010/1‑93 (MQ=255)

                                                 ctgagctgaTGCAAACGGCGCTAAACTGGGCGTATGCTCGCGGTAAAACAACTTTGCGGGTGGCGACCCAAATGGGCAACACCGCCGCGCTTa                                <  1:6026955/93‑1 (MQ=255)

                                                 ctgagctgaTGCAAACGGCGCTAAACTGGGCGTATGCTCGCGGTAAAACAACTTTGCGGGTGGCGACCCAAATGGGCAACACCGCCGCGCTTa                                <  1:6134996/93‑1 (MQ=255)

                                                  tgagctgaTGCAAACGGCGCTAAACTGGGCGTATGCTCGCGGTAAAACAACTTTGCGGGTGGCGACCCAAATGGGCAACACCGCCGCGCTTaa                               >  2:3567037/1‑93 (MQ=255)

                                                  tgagctgaTGCAAACGGCGCTAAACTGGGCGTATGCTCGCGGTAAAACAACTTTGCGGGTGGCGACCCAAATGGGCAACACCGCCGCGCTTaa                               <  1:434069/93‑1 (MQ=255)

                                                   gagctgaTGCAAACGGCGCTAAACTGGGCGTATGCTCGCGGTAAAACAACTTTGCGGGTGGCGACCCAAATGGGCAACACCGCCGCGCTTaaa                              >  1:5980927/1‑93 (MQ=255)

                                                      ctgaTGCAAACGGCGCTAAACTGGGCGTATGCTCGCGGTAAAACAACTTTGCGGGTGGCGACCCAAATGGGCAACACCGCCGCGCTTAAACGa                           >  2:623218/1‑93 (MQ=255)

                                                      ctgaTGCAAACGGCGCTAAACTGGGCGTATGCTCGCGGTAAAACAACTTTGCGGGTGGCGACCCAAATGGGCAACACCGCCGCGCTTAAACGa                           >  2:1766894/1‑93 (MQ=255)

                                                      ctgaTGCAAACGGCGCTAAACTGGGCGTATGCTCGCGGTAAAACAACTTTGCGGGTGGCGACCCAAATGGGCAACACCGCCGCGCTTAAACGa                           <  2:3405220/93‑1 (MQ=255)

                                                        gaTGCAAACGGCGCTAAACTGGGCGTATGCTCGCGGTAAAACAACTTTGCGGGTGGCGACCCAAATGGGCAACACCGCCGCGCTTAAACGata                         >  2:6401404/1‑93 (MQ=255)

                                                        gaTGCAAACGGCGCTAAACTGGGCGTATGCTCGCGGTAAAACAACTTTGCGGGTGGCGACCCAAATGGGCAACACCGCCGCGCTTAAACGata                         <  2:275909/93‑1 (MQ=255)

                                                        gaTGCAAACGGCGCTAAACTGGGCGTATGCTCGCGGTAAAACAACTTTGCGGGTGGCGACCCAAATGGGCAACACCGCCGCGCTTAAACGata                         >  1:463418/1‑93 (MQ=255)

                                                         aTGCAAACGGCGCTAAACTGGGCGTATGCTCGCGGTAAAACAACTTTGCGGGTGGCGACCCAAATGGGCAACACCGCCGCGCTTAAACGatac                        <  1:1414324/93‑1 (MQ=255)

                                                         aTGCAAACGGCGCTAAACTGGGCGTATGCTCGCGGTAAAACAACTTTGCGGGTGGCGACCCAAATGGGCAACACCGCCGCGCTTAAACGatac                        >  2:4834207/1‑93 (MQ=255)

                                                           gCAAACGGCGCTAAACTGGGCGTATGCTCGCGGTAAAACAACTTTGCGGGTGGCGACCCAAATGGGCAACACCGCCGCGCTTAAACGatacat                      <  2:2162299/93‑1 (MQ=255)

                                                             aaaCGGCGCTAAACTGGGCGTATGCTCGCGGTAAAACAACTTTGCGGGTGGCGACCCAAATGGGCAACACCGCCGCGCTTAAACGatacatac                    <  2:4115940/93‑1 (MQ=255)

                                                              aaCGGCGCTAAACTGGGCGTATGCTCGCGGTAAAACAACTTTGCGGGTGGCGACCCAAATGGGCAACACCGCCGCGCTTAAACGAtacataca                   <  1:2354985/93‑1 (MQ=255)

                                                              aaCGGCGCTAAACTGGGCGTATGCTCGCGGTAAAACAACTTTGCGGGTGGCGACCCAAATGGGCAACACCGCCGCGCTTAAACGAtacataca                   >  1:5398973/1‑93 (MQ=255)

                                                               aCGGCGCTAAACTGGGCGTATGCTCGCGGTAAAACAACTTTGCGGGTGGCGACCCAAATGGGCAACACCGCCGCGCTTAAACGATACATACaa                  >  1:2645461/1‑93 (MQ=255)

                                                               aCGGCGCTAAACTGGGCGTATGCTCGCGGTAAAACAACTTTGCGGGTGGCGACCCAAATGGGCAACACCGCCGCGCTTAAACGATACATACaa                  >  2:4921312/1‑93 (MQ=255)

                                                               aCGGCGCTAAACTGGGCGTATGCTCGCGGTAAAACAACTTTGCGGGTGGCGACCCAAATGGGCAACACCGCCGCGCTTAAACGATACATACaa                  >  1:3302289/1‑93 (MQ=255)

                                                                cGGCGCTAAACTGGGCGTATGCTCGCGGTAAAACAACTTTGCGGGTGGCGACCCAAATGGGCAACACCGCCGCGCTTAAACGATACATACaaa                 <  2:2836146/93‑1 (MQ=255)

                                                                  gcgcTAAACTGGGCGTATGCTCGCGGTAAAACAACTTTGCGGGTGGCGACCCAAATGGGCAACACCGCCGCGCTTAAACGATACATACAAAgt               <  1:709080/93‑1 (MQ=255)

                                                                  gcgcTAAACTGGGCGTATGCTCGCGGTAAAACAACTTTGCGGGTGGCGACCCAAATGGGCAACACCGCCGCGCTTAAACGATACATACAAAgt               <  1:3961090/93‑1 (MQ=255)

                                                                  gcgcTAAACTGGGCGTATGCTCGCGGTAAAACAACTTTGCGGGTGGCGACCCAAATGGGCAACACCGCCGCGCTTAAACGATACATACAAAgt               <  2:1925983/93‑1 (MQ=255)

                                                                   cgcTAAACTGGGCGTATGCTCGCGGTAAAACAACTTTGCGGGTGGCGACCCAAATGGGCAACACCGCCGCGCTTAAACGATACATACAAAgtg              <  1:995557/93‑1 (MQ=255)

                                                                    gcTAAACTGGGCGTATGCTCGCGGTAAAACAACTTTGCGGGTGGCGACCCAAATGGGCAACACCGCCGCGCTTAAACGATACATACAAAgtgg             >  1:2375336/1‑93 (MQ=255)

                                                                    gcTAAACTGGGCGTATGCTCGCGGTAAAACAACTTTGCGGGTGGCGACCCAAATGGGCAACACCGCCGCGCTTAAACGATACATACAAAgtgg             <  2:500506/93‑1 (MQ=255)

                                                                    gcTAAACTGGGCGTATGCTCGCGGTAAAACAACTTTGCGGGTGGCGACCCAAATGGGCAACACCGCCGCGCTTAAACGATACATACAAAgtgg             >  1:3745123/1‑93 (MQ=255)

                                                                     cTAAACTGGGCGTATGCTCGCGGTAAAACAACTTTGCGGGTGGCGACCCAAATGGGCAACACCGCCGCGCTTAAACGATACATACAAAgtggt            >  2:1384214/1‑93 (MQ=255)

                                                                     cTAAACTGGGCGTATGCTCGCGGTAAAACAACTTTGCGGGTGGCGACCCAAATGGGCAACACCGCCGCGCTTAAACGATACATACAAAgtggt            >  2:2553491/1‑93 (MQ=255)

                                                                     cTAAACTGGGCGTATGCTCGCGGTAAAACAACTTTGCGGGTGGCGACCCAAATGGGCAACACCGCCGCGCTTAAACGATACATACAAAgtggt            <  2:1812823/93‑1 (MQ=255)

                                                                     cTAAACTGGGCGTATGCTCGCGGTAAAACAACTTTGCGGGTGGCGACCCAAATGGGCAACACCGCCGCGCTTAAACGATACATACAAAgtggt            <  2:4558720/93‑1 (MQ=255)

                                                                      tAAACTGGGCGTATGCTCGCGGTAAAACAACTTTGCGGGTGGCGACCCAAATGGGCAACACCGCCGCGCTTAAACGATACATACAAAgtggtg           >  2:425795/1‑93 (MQ=255)

                                                                      tAAACTGGGCGTATGCTCGCGGTAAAACAACTTTGCGGGTGGCGACCCAAATGGGCAACACCGCCGCGCTTAAACGATACATACAAAgtggtg           >  1:3935256/1‑93 (MQ=255)

                                                                      tAAACTGGGCGTATGCTCGCGGTAAAACAACTTTGCGGGTGGCGACCCAAATAGGCAACCCCGCCGCGCTTAAACGATACATACAAAgtgttg           >  1:4175712/1‑93 (MQ=255)

                                                                         aCTGGGCGTATGCTCGCGGTAAAACAACTTTGCGGGTGGCGACCCAAATGGGCAACACCGCCGCGCTTAAACGATACATACAAAGTGGTGCGa        >  1:215530/1‑93 (MQ=255)

                                                                          cTGGGCGTATGCTCGCGGTAAAACAACTTTGCGGGTGGCGACCCAAATGGGCAACACCGCCGCGCTTAAACGATACATACAAAGTGGTGCGaa       <  1:4052535/93‑1 (MQ=255)

                                                                          cTGGGCGTATGCTCGCGGTAAAACAACTTTGCGGGTGGCGACCCAAATGGGCAACACCGCCGCGCTTAAACGATACATACAAAGTGGTGCGaa       >  1:1004509/1‑93 (MQ=255)

                                                                           tGGGCGTATGCTCGCGGTAAAACAACTTTGCGGGTGGCGACCCAAATGGGCAACACCGCCGCGCTTAAACGATACATACAAAGTGGTGCGAAt      >  2:1976587/1‑93 (MQ=255)

                                                                           tGGGCGTATGCTCGCGGTAAAACAACTTTGCGGGTCGCGACCCAAATGGGCAACACCGCCGCGCTTAAACGATACATACAAAGTGGTGCGAAt      <  2:3548603/93‑1 (MQ=255)

                                                                              gCGTATGCTCGCGGTAAAACAACTTTGCGGGTGGCGACCCAAATGGGCAACACCGCCGCGCTTAAACGATACATACAAAGTGGTGCGAATGTa   <  1:3620067/93‑1 (MQ=255)

                                                                              gCGTATGCTCGCGGTAAAACAACTTTGCGGGTGGCGACCCAAATGGGCAACACCGCCGCGCTTAAACGATACATACAAAGTGGTGCGAATGTa   <  1:5026163/93‑1 (MQ=255)

                                                                               cGTATGCTCGCGGTAAAACAACTTTGCGGGTGGCGACCCAAATGGGCAACACCGCCGCGCTTAAACGATACATACAAAGTGGTGCGAATGTAg  <  2:1035517/93‑1 (MQ=255)

||

AATGCGACAGATGCGCGAATTGGCCTGCTGGCTGGACGCGGTGCAGGTGCTGAGCTGATGCAAACGGCGCTAAACTGGGCGTATCGTCGCGGTAAAACAACTTTGCGGGTGGCGACCCAAATGGGCAACACCGCCGCGCTTAAACGATACATACAAAGTGGTGCGAATGTAG  >  NC_000913/3972553‑3972724

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 20 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

BRESEQ :: bam2aln output

CGGGAACTCAATGCGACAGATGCGCGAATTGGCCTGCTGGCTGGACGCGGTGCAGGTGCTGAGCTGATGCAAACGGCGCTAAACTGGGCGTATCGTCGCGGTAAAACAACTTTGCGGGTGGCGACCCAAATGGGCAACACCGCCGCGCTTAAACGATACATACAAAGTGGTGCGAATGTAGAA  >  NC_000913/3972544‑3972726

CGGGAACTCAATGCGACAGATGCGCGAATTGGCCTGCTGGCTGGACGCGGTGCAGGTGCTGAGCTGATGCAAACGGCGCTAAACTGGGCGTATG                                                                                           <  SRR4302537.261748/94‑1 (MQ=60)

       TCAATGCGACAGATGCGCGAATTGGCCTGCTGGCTGGACGCGGTGCAGGTGCTGAGCTGATGCAAACGGCGCTAAACTGGGCGTATGCTCGCGG                                                                                    <  SRR4302537.2209246/94‑1 (MQ=60)

         AATGCGACAGATGCGCGAATTGGCCTGCTGGCTGGACGCGGTGCAGGTGCTGAGCTGATGCAAACGGCGCTAAACTGGGCGTATGCTCGCGGTA                                                                                  >  SRR4302537.2570036/1‑94 (MQ=60)

           TGCGACAGATGCGCGAATTGGCCTGCTGGCTGGACGCGGTGCAGGTGCTGAGCTGATGCAAACGGCGCTAAACTGGGCGTATGCTCGCGGTAAA                                                                                <  SRR4302537.5668493/94‑1 (MQ=60)

             CGACAGATGCGCGAATTGGCCTGCTGGCTGGACGCGGTGCAGGTGCTGAGCTGATGCAAACGGCGCTAAACTGGGCGTATGCTCGCGGTAAAAC                                                                              >  SRR4302537.3328317/1‑94 (MQ=60)

              GACAGATGCGCGAATTGGCCTGCTGGCTGGACGCGGTGCAGGTGCTGAGCTGATGCAAACGGCGCTAAACTGGGCGTATGCTCGCGGTAAAACA                                                                             <  SRR4302537.5050532/94‑1 (MQ=60)

                CAGATGCGCGAATTGGCCTGCTGGCTGGACGCGGTGCAGGTGCTGAGCTGATGCAAACGGCGCTAAACTGGGCGTATGCTCGCGGTAAAACAAC                                                                           <  SRR4302537.2405198/94‑1 (MQ=60)

                  GATGCGCGAATTGGCCTGCTGGCTGGACGCGGTGCAGGTGCTGAGCTGATGCAAACGGCGCTAAACTGGGCGTATGCTCGCGGTAAAACAACTT                                                                         <  SRR4302537.533422/94‑1 (MQ=60)

                  GATGCGCGAATTGGCCTGCTGGCTGGACGCGGTGCAGGTGCTGAGCTGATGCAAACGGCGCTAAACTGGGCGTATGCTCGCGGTAAAACAACTT                                                                         <  SRR4302537.3432065/94‑1 (MQ=60)

                  GATGCGCGAATTGGCCTGCTGGCTGGACGCGGTGCAGGTGCTGAGCTGATGCAAACGGCGCTAAACTGGGCGTATGCTCGCGGTAAAACAACTT                                                                         <  SRR4302537.2981950/94‑1 (MQ=60)

                   ATGCGCGAATTGGCCTGCTGGCTGGACGCGGTGCAGGTGCTGAGCTGATGCAAACGGCGCTAAACTGGGCGTATGCTCGCGGTAAAACAACTTT                                                                        <  SRR4302537.3336066/94‑1 (MQ=60)

                   ATGCGCGAATTGGCCTGCTGGCTGGACGCGGTGCAGGTGCTGAGCTGATGCAAACGGCGCTAAACTGGGCGTATGCTCGCGGTAAAACAACTTT                                                                        <  SRR4302537.3894731/94‑1 (MQ=60)

                   ATGCGCGAATTGGCCTGCTGGCTGGACGCGGTGCAGGTGCTGAGCTGATGCAAACGGCGCTAAACTGGGCGTATGCTCGCGGTAAAACAACTTT                                                                        <  SRR4302537.3243474/94‑1 (MQ=60)

                   ATGCGCGAATTGGCCTGCTGGCTGGACGCGGTGCAGGTGCTGAGCTGATGCAAACGGCGCTAAACTGGGCGTATGCTCGCGGTAAAACAACTTT                                                                        <  SRR4302537.2377948/94‑1 (MQ=60)

                   ATGCGCGAATTGGCCTGCTGGCTGGACGCGGTGCAGGTGCTGAGCTGATGCAAACGGCGCTAAACTGGGCGTATGCTCGCGGTAAAACAACTTT                                                                        <  SRR4302537.4226280/94‑1 (MQ=60)

                    TGCGCGAATTGGCCTGCTGGCTGGACGCGGTGCAGGTGCTGAGCTGATGCAAACGGCGCTAAACTGGGCGTATGCTCGCGGTAAAACAACTTTG                                                                       <  SRR4302537.4142966/94‑1 (MQ=60)

                     GCGCGAATTGGCCTGCTGGCTGGACGCGGTGCAGGTGCTGAGCTGATGCAAACGGCGCTAAACTGGGCGTATGCTCGCGGTAAAACAACTTTGC                                                                      >  SRR4302537.6266757/1‑94 (MQ=60)

                      CGCGAATTGGCCTGCTGGCTGGACGCGGTGCAGGTGCTGAGCTGATGCAAACGGCGCTAAACTGGGCGTATGCTCGCGGTAAAACAACTTTGCG                                                                     >  SRR4302537.4031592/1‑94 (MQ=60)

                      CGCGAATTGGCCTGCTGGCTGGACGCGGTGCAGGTGCTGAGCTGATGCAAACGGCGCTAAACTGGGCGTATGCTCGCGGTAAAACAACTTTGCG                                                                     >  SRR4302537.2913776/1‑94 (MQ=60)

                        CGAATTGGCCTGCTGGCTGGACGCGGTGCAGGTGCTGAGCTGATGCAAACGGCGCTAAACTGGGCGTATGCTCGCGGTAAAACAACTTTGCGGG                                                                   >  SRR4302537.4465217/1‑94 (MQ=60)

                        CGAATTGGCCTGCTGGCTGGACGCGGTGCAGGTGCTGAGCTGATGCAAACGGCGCTAAACTGGGCGTATGCTCGCGGTAAAACAACTTTGCGGG                                                                   <  SRR4302537.1963972/94‑1 (MQ=60)

                         GAATTGGCCTGCTGGCTGGACGCGGTGCAGGTGCTGAGCTGATGCAAACGGCGCTAAACTGGGCGTATGCTCGCGGTAAAACAACTTTGCGGGT                                                                  <  SRR4302537.5454294/94‑1 (MQ=60)

                         GAATTGGCCTGCTGGCTGGACGCGGTGCAGGTGCTGAGCTGATGCAAACGGCGCTAAACTGGGCGTATGCTCGCGGTAAAACAACTTTGCGGGT                                                                  >  SRR4302537.5070273/1‑94 (MQ=60)

                          AATTGGCCTGCTGGCTGGACGCGGTGCAGGTGCTGAGCTGATGCAAACGGCGCTAAACTGGGCGTATGCTCGCGGTAAAACAACTTTGCGGGTG                                                                 >  SRR4302537.4270013/1‑94 (MQ=60)

                          AATTGGCCTGCTGGCTGGACGCGGTGCAGGTGCTGAGCTGATGCAAACGGCGCTAAACTGGGCGTATGCTCGCGGTAAAACAACTTTGCGGGTG                                                                 <  SRR4302537.642696/94‑1 (MQ=60)

                          AATTGGCCTGCTGGCTGGACGCGGTGCAGGTGCTGAGCTGATGCAAACGGCGCTAAACTGGGCGTATGCTCGCGGTAAAACAACTTTGCGGGTG                                                                 <  SRR4302537.4337444/94‑1 (MQ=60)

                          AATTGGCCTGCTGGCTGGACGCGGTGCAGGTGCTGAGCTGATGCAAACGGCGCTAAACTGGGCGTATGCTCGCGGTAAAACAACTTTGCGGGTG                                                                 >  SRR4302537.6184456/1‑94 (MQ=60)

                          AATTGGCCTGCTGGCTGGACGCGGTGCAGGTGCTGAGCTGATGCAAACGGCGCTAAACTGGGCGTATGCTCGCGGTAAAACAACTTTGCGGGTG                                                                 >  SRR4302537.1152803/1‑94 (MQ=60)

                           ATTGGCCTGCTGGCTGGACGCGGTGCAGGTGCTGAGCTGATGCAAACGGCGCTAAACTGGGCGTATGCTCGCGGTAAAACAACTTTGCGGGTGG                                                                <  SRR4302537.5303266/94‑1 (MQ=60)

                           ATTGGCCTGCTGGCTGGACGCGGTGCAGGTGCTGAGCTGATGCAAACGGCGCTAAACTGGGCGTATGCTCGCGGTAAAACAACTTTGCGGGTGG                                                                <  SRR4302537.5992312/94‑1 (MQ=60)

                           ATTGGCCTGCTGGCTGGACGCGGTGCAGGTGCTGAGCTGATGCAAACGGCGCTAAACTGGGCGTATGCTCGCGGTAAAACAACTTTGCGGGTGG                                                                <  SRR4302537.5031479/94‑1 (MQ=60)

                              GGCCTGCTGGCTGGACGCGGTGCAGGTGCTGAGCTGATGCAAACGGCGCTAAACTGGGCGTATGCTCGCGGTAAAACAACTTTGCGGGTGGCGA                                                             >  SRR4302537.2079023/1‑94 (MQ=60)

                               GCCTGCTGGCTGGACGCGGTGCAGGTGCTGAGCTGATGCCAACGGCGCTAAACTGGGCGTATGCTCGCGGTAAAACAACTTTGCGGGTGGCGAC                                                            <  SRR4302537.5037590/94‑1 (MQ=60)

                               GCCTGCTGGCTGGACGCGGTGCAGGTGCTGAGCTGATGCAAACGGCGCTAAACTGGGCGTATGCTCGCGGTAAAACAACTTTGCGGGTGGCGAC                                                            <  SRR4302537.5037591/94‑1 (MQ=60)

                                 CTGCTGGCTGGACGCGGTGCAGGTGCTGAGCTGATGCAAACGGCGCTAAACTGGGCGTATGCTCGCGGTAAAACAACTTTGCGGGTGGCGACCC                                                          >  SRR4302537.262272/1‑94 (MQ=60)

                                  TGCTGGCTGGACGCGGTGCAGGTGCTGAGCTGATGCAAACGGCGCTAAACTGGGCGTATGCTCGCGGTAAAACAACTTTGCGGGTGGCGACCCA                                                         >  SRR4302537.4331179/1‑94 (MQ=60)

                                    CTGGCTGGACGCGGTGCAGGTGCTGAGCTGATGCAAACGGCGCTAAACTGGGCGTATGCTCGCGGTAAAACAACTTTGCGGGTGGCGACCCAAA                                                       <  SRR4302537.5005794/94‑1 (MQ=60)

                                      GGCTGGACGCGGTGCAGGTGCTGAGCTGATGCAAACGGCGCTAAACTGGGCGTATGCTCGCGGTAAAACAACTTTGCGGGTGGCGACCCAAATG                                                     <  SRR4302537.5690213/94‑1 (MQ=60)

                                      GGCTGGACGCGGTGCAGGTGCTGAGCTGATGCAAACGGCGCTAAACTGGGCGTATGCTCGCGGTAAAACAACTTTGCGGGTGGCGACCCAAATG                                                     <  SRR4302537.3579389/94‑1 (MQ=60)

                                      GGCTGGACGCGGTGCAGGTGCTGAGCTGATGCAAACGGCGCTAAACTGGGCGTATGCTCGCGGTAAAACAACTTTGCGGGTGGCGACCCAAATG                                                     <  SRR4302537.3169594/94‑1 (MQ=60)

                                       GCTGGACGCGGTGCAGGTGCTGAGCTGATGCAAACGGCGCTAAACTGGGCGTATGCTCGCGGTAAAACAACTTTGCGGGTGGCGACCCAAATGG                                                    <  SRR4302537.2991787/94‑1 (MQ=60)

                                          GGCCGCGGTGCAGGTGCTGAGCTGATGCAAACGGCGCTAAACTGGGCGTATGCTCGCGGTAAAACAACTTTGCGGGTGGCGACCCAAATGGGCA                                                 <  SRR4302537.763795/94‑1 (MQ=60)

                                          GGACGCGGTGCAGGTGCTGAGCTGATGCAAACGGCGCTAAACTGGGCGTATGCTCGCGGTAAAACAACTTTGCGGGTGGCGACCCAAATGGGCA                                                 <  SRR4302537.5902376/94‑1 (MQ=60)

                                           GACGCGGTGCAGGTGCTGAGCTGATGCAAACGGCGCTAAACTGGGCGTATGCTCGTGGTAAAACAACTTTGCGGGTGGCGACCCAAATGGGCAA                                                >  SRR4302537.5110523/1‑94 (MQ=60)

                                             CGCGGTGCAGGTGCTGAGCTGATGCAAACGGCGCTAAACTGGGCGTATGCTCGCGGTAAAACAACTTTGCGGGTGGCGACCCAAATGGGCAACA                                              <  SRR4302537.3814256/94‑1 (MQ=60)

                                               CGGTGCAGGTGCTGAGCTGATGCAAACGGCGCTAAACTGGGCGTATGCTCGCGGTAAAACAACTTTGCGGGTGGCGACCCAAATGGGCAACACC                                            <  SRR4302537.575364/94‑1 (MQ=60)

                                                   GCAGGTGCTGAGCTGATGCAAACGGCGCTAAACTGGGCGTATGCTCGCGGTAAAACAACTTTGCGGGTGGCGACCCAAATGGGCAACACCGCCG                                        <  SRR4302537.2585744/94‑1 (MQ=60)

                                                   GCAGGTGCTGAGCTGATGCAAACGGCGCTAAACTGGGCGTATGCTCGCGGTAAAACAACTTTGCGGGTGGCGACCCAAATGGGCAACACCGCCG                                        >  SRR4302537.3646592/1‑94 (MQ=60)

                                                    CAGGTGCTGAGCTGATGCAAACGGCGCTAAACTGGGCGTATGCTCGCGGTAAAACAACTTTGCGGGTGGCGACCCAAATGGGCAACACCGCCGC                                       >  SRR4302537.3954073/1‑94 (MQ=60)

                                                    CAGGTGCTGAGCTGATGCAAACGGCGCTAAACTGGGCGTATGCTCGCGGTAAAACAACTTTGCGGGTGGCGACCCAAATGGGCAACACCGCCGC                                       >  SRR4302537.37381/1‑94 (MQ=60)

                                                    CAGGTGCTGAGCTGATGCAAACGGCGCTAAACTGGGCGTATGCTCGCGGTAAAACAACTTTGCGGGTGGCGACCCAAATGGGCAACACCGCCGC                                       >  SRR4302537.2931035/1‑94 (MQ=60)

                                                    CAGGTGCTGAGCTGATGCAAACGGCGCTAAACTGGGCGTATGCTCGCGGTAAAACAACTTTGCGGGTGGCGACCCAAATGGGCAACACCGCCGC                                       >  SRR4302537.5375475/1‑94 (MQ=60)

                                                     AGGTGCTGAGCTGATGCAAACGGCGCTAAACTGGGCGTATGCTCGCGGTAAAACAACTTTGCGGGTGGCGACCCAAATGGGCAACACCGCCGCG                                      >  SRR4302537.1532256/1‑94 (MQ=60)

                                                      GGTGCTGAGCTGATGCAAACGGCGCTAAACTGGGCGTATGCTCGCGGTAAAACAACTTTGCGGGTGGCGACCCAAATGGGCAACACCGCCGCGC                                     >  SRR4302537.892638/1‑94 (MQ=60)

                                                      GGTGCTGAGCTGATGCAAACGGCGCTAAACTGGGCGTATGCTCGCGGTAAAACAACTTTGCGGGTGGCGACCCAAATGGGCAACACCGCCGCGC                                     <  SRR4302537.4795893/94‑1 (MQ=60)

                                                       GTGCTGAGCTGATGCAAACGGCGCTAAACTGGGCGTATGCTCGCGGTAAAACAACTTTGCGGGTGGCGACCCAAATGGGCAACACCGCCGCGCT                                    >  SRR4302537.5450407/1‑94 (MQ=60)

                                                       GTGCTGAGCTGATGCAAACGGCGCTAAACTGGGCGTATGCTCGCGGTAAAACAACTTTGCGGGTGGCGACCCAAATGGGCAACACCGCCGCGCT                                    >  SRR4302537.3718461/1‑94 (MQ=60)

                                                       GTGCTGAGCTGATGCAAACGGCGCTAAACTGGGCGTATGCTCGCGGTAAAACAACTTTGCGGGTGGCGACCCAAATGGGCAACACCGCCGCGCT                                    <  SRR4302537.4906994/94‑1 (MQ=60)

                                                        TGCTGAGCTGATGCAAACGGCGCTAAACTGGGCGTATGCTCGCGGTAAAACAACTTTGCGGGTGGCGACCCAAATGGGCAACACCGCCGCGCTT                                   >  SRR4302537.2907273/1‑94 (MQ=60)

                                                          CTGAGCTGATGCAAACGGCGCTAAACTGGGCGTATGCTCGCGGTAAAACAACTTTGCGGGTGGCGACCCAAATGGGCAACACCGCCGCGCTTAA                                 <  SRR4302537.6068368/94‑1 (MQ=60)

                                                          CTGAGCTGATGCAAACGGCGCTAAACTGGGCGTATGCTCGCGGTAAAACAACTTTGCGGGTGGCGACCCAAATGGGCAACACCGCCGCGCTTAA                                 <  SRR4302537.6177173/94‑1 (MQ=60)

                                                           TGAGCTGATGCAAACGGCGCTAAACTGGGCGTATGCTCGCGGTAAAACAACTTTGCGGGTGGCGACCCAAATGGGCAACACCGCCGCGCTTAAA                                <  SRR4302537.437097/94‑1 (MQ=60)

                                                           TGAGCTGATGCAAACGGCGCTAAACTGGGCGTATGCTCGCGGTAAAACAACTTTGCGGGTGGCGACCCAAATGGGCAACACCGCCGCGCTTAAA                                >  SRR4302537.6021978/1‑94 (MQ=60)

                                                                TGATGCAAACGGCGCTAAACTGGGCGTATGCTCGCGGTAAAACAACTTTGCGGGTGGCGACCCAAATGGGCAACACCGCCGCGCTTAAACGATA                           >  SRR4302537.466607/1‑94 (MQ=60)

                                                                TGATGCAAACGGCGCTAAACTGGGCGTATGCTCGCGGTAAAACAACTTTGCGGGTGGCGACCCAAATGGGCAACACCGCCGCGCTTAAACGATA                           <  SRR4302537.1471398/94‑1 (MQ=60)

                                                                TGATGCAAACGGCGCTAAACTGGGCGTATGCTCGCGGTAAAACAACTTTGCGGGTGGCGACCCAAATGGGCAACACCGCCGCGCTTAAACGATA                           <  SRR4302537.2104909/94‑1 (MQ=60)

                                                                  ATGCAAACGGCGCTAAACTGGGCGTATGCTCGCGGTAAAACAACTTTGCGGGTGGCGACCCAAATGGGCAACACCGCCGCGCTTAAACGATACA                         <  SRR4302537.1424168/94‑1 (MQ=60)

                                                                   TGCAAACGGCGCTAAACTGGGCGTATGCTCGCGGTAAAACAACTTTGCGGCTGGCGACCCAAATGGGCAACACCGCCGCGCTTAAACGATACAT                        <  SRR4302537.4586177/94‑1 (MQ=60)

                                                                     CAAACGGCGCTAAACTGGGCGTATGCTCGCGGTAAAACAACTTTGCGGGTGGCGACCCAAATGGGCAACACCGCCGCGCTTAAACGATACATAC                      >  SRR4302537.2578274/1‑94 (MQ=60)

                                                                      AAACGGCGCTAAACTGGGCGTATGCTCGCGGTAAAACAACTTTGCGGGTGGCGACCCAAATGGGCAACACCGCCGCGCTTAAACGATACATACA                     >  SRR4302537.5435826/1‑94 (MQ=60)

                                                                       AACGGCGCTAAACTGGGCGTATGCTCGCGGTAAAACAACTTTGCGGGTGGCGACCCAAATGGGCAACACCGCCGCGCTTAAACGATACATACAA                    <  SRR4302537.677380/94‑1 (MQ=60)

                                                                       AACGGCGCTAAACTGGGCGTATGCTCGCGGTAAAACAACTTTGCGGGTGGCGACCCAAATGGGCAACACCGCCGCGCTTAAACGATACATACAA                    >  SRR4302537.3325637/1‑94 (MQ=60)

                                                                       AACGGCGCTAAACTGGGCGTATGCTCGCGGTAAAACAACTTTGCGGGTGGCGACCCAAATGGGCAACACCGCCGCGCTTAAACGATACATACAA                    >  SRR4302537.2663963/1‑94 (MQ=60)

                                                                       AACGGCGCTAAACTGGGCGTATGCTCGCGGTAAAACAACTTTGCGGGTGGCGACCCAAATGGGCAACACCGCCGCGCTTAAACGATACATACAA                    <  SRR4302537.2371589/94‑1 (MQ=60)

                                                                          GGCGCTAAACTGGGCGTATGCTCGCGGTAAAACAACTTTGCGGGTGGCGACCCAAATGGGCAACACCGCCGCGCTTAAACGATACATACAAAGT                 <  SRR4302537.5768956/94‑1 (MQ=60)

                                                                           GCGCTAAACTGGGCGTATGCTCGCGGTAAAACAACTTTGCGGGTGGCGACCCAAATGGGCAACACCGCCGCGCTTAAACGATACATACAAAGTG                <  SRR4302537.3988800/94‑1 (MQ=60)

                                                                           GCGCTAAACTGGGCGTATGCTCGCGGTAAAACAACTTTGCGGGTGGCGACCCAAATGGGCAACACCGCCGCGCTTAAACGATACATACAAAGTG                <  SRR4302537.713932/94‑1 (MQ=60)

                                                                           GCGCGAAACTGGGCGTATGCTCGCGGTAAAACAACTTTGCGGGTGGCGACCCAAATGGGCAACACCGCCGCGCTTAAACGATACATACAAAGTG                <  SRR4302537.3618041/94‑1 (MQ=60)

                                                                            CGCTAAACTGGGCGTATGCTCGCGGTAAAACAACTTTGCGGGTGGCGACCCAAATGGGCAACACCGCCGCGCTTAAACGATACATACAAAGTGG               >  SRR4302537.3771421/1‑94 (MQ=60)

                                                                            CGCTAAACTGGGCGTATGCTCGCGGTAAAACAACTTTGCGGGTGGCGACCCAAATGGGCAACACCGCCGCGCTTAAACGATACATACAAAGTGG               >  SRR4302537.2392074/1‑94 (MQ=60)

                                                                            CGCTAAACTGGGCGTATGCTCGCGGTAAAACAACTTTGCGGGTGGCGACCCAAATGGGCAACACCGCCGCGCTTAAACGATACATACAAAGTGG               <  SRR4302537.1272648/94‑1 (MQ=60)

                                                                            CGCTAAACTGGGCGTATGCTCGCGGTAAAACAACTTTGCGGGTGGCGACCCAAATGGGCAACACCGCCGCGCTTAAACGATACATACAAAGTGG               <  SRR4302537.1002434/94‑1 (MQ=60)

                                                                             GCTAAACTGGGCGTATGCTCGCGGTAAAACAACTTTGCGGGTGGCGACCCAAATGGGCAACACCGCCGCGCTTAAACGATACATACAAAGTGGT              >  SRR4302537.6420919/1‑94 (MQ=60)

                                                                              CTAAACTGGGCGTATGCTCGCGGTAAAACAACTTTGCGGGTGGCGACCCAAATGGGCAACACCGCCGCGCTTAAACGATACATACAAAGTGGTG             >  SRR4302537.3962759/1‑94 (MQ=60)

                                                                              CTAAACTGGGCGTATGCTCGCGGTAAAACAACTTTGCGGGTGGCGACCCAAATAGGCAACCCCGCCGCGCTTAAACGATACATACAAAGTGTTG             >  SRR4302537.4204866/1‑94 (MQ=60)

                                                                               TAAACTGGGCGTATGCTCGCGGTAAAACAACTTTGCGGGTGGCGACCCAAATGGGCAACACCGCCGCGCTTAAACGATACATACAAAGTGGTGC            <  SRR4302537.937743/94‑1 (MQ=60)

                                                                                AAACTGGGCGTATGCTCGCGGTAAAACAACTTTGCGGGTGGCGACCCAAATGGGCAACACCGCCGCGCTTAAACGATACATACAAAGTGGTGCG           <  SRR4302537.4353009/94‑1 (MQ=60)

                                                                                 AACTGGGCGTATGCTCGCGGTAAAACAACTTTGCGGGTGGCGACCCAAATGGGCAACACCGCCGCGCTTAAACGATACATACAAAGTGGTGCGA          >  SRR4302537.217069/1‑94 (MQ=60)

                                                                                  ACTGGGCGTATGCTCGCGGTAAAACAACTTTGCGGGTGGCGACCCAAATGGGCAACACCGCCGCGCTTAAACGATACATACAAAGTGGTGCGAA         >  SRR4302537.1011442/1‑94 (MQ=60)

                                                                                   CTGGGCGTATGCTCGCGGTAAAACAACTTTGCGGGTGGCGACCCAAATGGGCAACACCGCCGCGCTTAAACGATACATACAAAGTGGTGCGAAT        <  SRR4302537.4080872/94‑1 (MQ=60)

                                                                                     GGGCGTATGCTCGCGGTAAAACAACTTTGCGGGTGGCGACCCAAATGGGCAACACCGCCGCGCTTAAACGATACATACAAAGTGGTGCGAATGT      >  SRR4302537.6027655/1‑94 (MQ=60)

                                                                                      GGCGTATGCTCGCGGTAAAACAACTTTGCGGGTGGCGACCCAAATGGGCAACACCGCCGCGCTTAAACGATACATACAAAGTGGTGCGAATGTA     <  SRR4302537.2253588/94‑1 (MQ=60)

                                                                                      GGCGTATGCTCGCGGTAAAACAACTTTGCGGGTGGCGACCCAAATGGGCAACACCGCCGCGCTTAAACGATACATACAAAGTGGTGCGAATGTA     >  SRR4302537.2515544/1‑94 (MQ=60)

                                                                                      GGCGTATGCTCGCGGTAAAACAACTTTGCGGGTGGCGACCCAAATGGGCAACACCGCCGCGCTTAAACGATACATACAAAGTGGTGCGAATGTA     <  SRR4302537.2895207/94‑1 (MQ=60)

                                                                                      GGCGTATGCTCGCGGTAAAACAACTTTGCGGGTGGCGACCAAAATGGGCAACACCGCCGCGCTTAAACGATACATACAAAGTGGTGCGAATGTA     <  SRR4302537.4388687/94‑1 (MQ=60)

                                                                                       GCGTATGCTCGCGGTAAAACAACTTTGCGGGTGGCGACCCAAATGGGCAACACCGCCGCGCTTAAACGATACATACAAAGTGGTGCGAATGTAG    >  SRR4302537.5364815/1‑94 (MQ=60)

                                                                                       GCGTATGCTCGCGGTAAAACAACTTTGCGGGTGGCGACCCAAATGGGCAACACCGCCGCGCTTAAACGATACATACAAAGTGGTGCGAATGTAG    <  SRR4302537.3645582/94‑1 (MQ=60)

                                                                                       GCGTATGCTCGCGGTAAAACAACTTTGCGGGTGGCGACCCAAATGGGCAACACCGCCGCGCTTAAACGATACATACAAAGTGGTGCGAATGTAG    <  SRR4302537.5060776/94‑1 (MQ=60)

                                                                                        CGTATGCTCGCGGTAAAACAACTTTGCGGGTGGCGACCCAAATGGGCAACACCGCCGCGCTTAAACGATACATACAAAGTGGTGCGAATGTAGA   >  SRR4302537.4249600/1‑94 (MQ=60)

                                                                                        CGTATGCTCGCGGTAAAACAACTTTGCGGGTGGCGACCCAAATGGGCAACACCGCCGCGCTTAAACGATACATACAAAGTGGTGCGAATGTAGA   >  SRR4302537.2431079/1‑94 (MQ=60)

                                                                                        CGTATGCTCGCGGTAAAACAACTTTGCGGGTGGCGACCCAAATGGGCAACACCGCCGCGCTTAAACGATACATACAAAGTGGTGCGAATGTAGA   >  SRR4302537.4336095/1‑94 (MQ=60)

                                                                                        CGTATGCTCGCGGTAAAACAACTTTGCGGGTGGCGACCCAAATGGGCAACACCGCCGCGCTTAAACGATACATACAAAGTGGTGCGAATGTAGA   <  SRR4302537.5563524/94‑1 (MQ=60)

                                                                                         GTATGCTCGCGGTAAAACAACTTTGCGGGTGGCGACCCAAATGGGCAACACCGCCGCGCTTAAACGATACATACAAAGTGGTGCGAATGTAGAA  >  SRR4302537.403195/1‑94 (MQ=60)

                                                                                         GTATGCTCGCGGTAAAACAACTTTGCGGGTGGCGACCCAAATGGGCAACACCGCCGCGCTTAAACGATACATACAAAGTGGTGCGAATGTAGAA  >  SRR4302537.6512568/1‑94 (MQ=60)

CGGGAACTCAATGCGACAGATGCGCGAATTGGCCTGCTGGCTGGACGCGGTGCAGGTGCTGAGCTGATGCAAACGGCGCTAAACTGGGCGTATCGTCGCGGTAAAACAACTTTGCGGGTGGCGACCCAAATGGGCAACACCGCCGCGCTTAAACGATACATACAAAGTGGTGCGAATGTAGAA  >  NC_000913/3972544‑3972726

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 0 ≤` `ATCG/ATCG` `< 17 ≤` `ATCG/ATCG` `< 28 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`