Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I2 R1 882 254.4 12957588 98.0% 12698436 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,978,702 C→T S68F (TCC→TTC)  yifK → putative amino acid/amine transport protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,978,7020CT99.6% 947.2 / NA 270S68F (TCC→TTC) yifKputative amino acid/amine transport protein
Reads supporting (aligned to +/- strand):  ref base C (0/1);  major base T (134/134);  minor base G (0/1);  total (134/136)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 280 total aligned reads displayed.

TGAAATGGGCCGGGCCATCCGTATTGTTGGCCTATATCATCGCCGGGCTGTTCGTCTTTTTCATCATGCGTTCAATGGGCGAAATGTTGTCCCTCGAACCGGTTACCGGTTCGTTCGCCGTTTATGCGCATCGTTATATGAGCCCGTTCTTTGGCTATCTCACCGCCTGGTCTTACTGGT  >  NC_000913/3978612‑3978791
                                                                                          |                                                                                         
 gAAATGGGCCGGGCCATCCGTATTGTTGGCCTATATCATCGCCGGGCTGTTCGTCTTTTTCATCATGCGTTCAATGGGCGAAATGTTGTTCCt                                                                                        <  2:4847357/93‑1 (MQ=255)
 gAAATGGGCCGGGCCATCCGTATTGTTGGCCTATATCATCGCCGGGCTGTTCGTCTTTTTCATCATGCGTTCAATGGGCGAAATGTTGTTCCt                                                                                        >  1:1952992/1‑93 (MQ=255)
  aaaTGGGCCGGGCCATCCGTATTGTTGGCCTATATCATCGCCGGGCTGTTCGTCTTTTTCATCATGCGTTCAATGGGCGAAATGTTGTTCCTc                                                                                       <  1:3141104/93‑1 (MQ=255)
   aaTGGGCCGGGCCATCCGTATTGTTGGCCTATATCATCGCCGGGCTGTTCGTCTTTTTCATCATGCGTTCAATGGGCGAAATGTTGTTCCTCg                                                                                      >  2:5662387/1‑93 (MQ=255)
   aaTGGGCCGGGCCATCCGTATTGTTGGCCTATATCATCGCCGGGCTGTTCGTCTTTTTCATCATGCGTTCAATGGGCGAAATGTTGTTCCTCg                                                                                      >  2:1077939/1‑93 (MQ=255)
    aTGGGCCGGGCCATCCGTATTGTTGGCCTATATCATCGCCGGGCTGTTCGTCTTTTTCATCATGCGTTCAATGGGCGAAATGTTGTTCCTCGa                                                                                     >  1:242776/1‑93 (MQ=255)
     tGGGCCGGGCCATCCGTATTGTTGGCCTATATCATCGCCGGGCTGTTCGTCTTTTTCATCATGCGTTCAATGGGCGAAATGTTGTTCCTCGaa                                                                                    <  2:1070014/93‑1 (MQ=255)
     tGGGCCGGGCCATCCGTATTGTTGGCCTATATCATCGCCGGGCTGTTCGTCTTTTTCATCATGCGTTCAATGGGCGAAATGTTGTTCCTCGaa                                                                                    >  1:2086098/1‑93 (MQ=255)
     tGGGCCGGGCCATCCGTATTGTTGGCCTATATCATCGCCGGGCTGTTCGTCTTTTTCATCATGCGTTCAATGGGCGAAATGTTGTTCCTCGaa                                                                                    <  2:4493540/93‑1 (MQ=255)
      ggtccgggcaATCCGTATTGTTGGCCTATATCATCGCCGGGCTGTTCGTCTTTTTCATCATGCGTTCAATGGGCGAAATGTTGTTCCTCGAac                                                                                   <  2:3679562/90‑1 (MQ=255)
      gggccgggccATCCGTATTGTTGGCCTATATCATCGCCGGGCTGTTCGTCTTTTTCATCATGCGTTCAATGGGCGAAATGTTGTTCCTCGAac                                                                                   <  1:3008227/93‑1 (MQ=255)
       ggccgggccATCCGTATTGTTGGCCTATATCATCGCCGGGCTGTTCGTCTTTTTCATCATGCGTTCAATGGGCGAAATGTTGTTCCTCGAacc                                                                                  <  2:4699579/93‑1 (MQ=255)
       ggccgggccATCCGTATTGTTGGCCTAAATCCTCGCCAGGCTGTTCATCTTTTTCATCATGCGTTCAATGGGCGAAATGTTGTTCCTCGAacc                                                                                  <  1:6471109/93‑1 (MQ=255)
          cgggccATCCGTATTGTTGGCCTATATCATCGCCGGGCTGTTCGTCTTTTTCATCATGCGTTCAATGGGCGAAATGTTGTTCCTCGAaccggt                                                                               >  1:3164816/1‑93 (MQ=255)
          cgggccATCCGTATTGTTGGCCTATATCATCGCCGGGCTGTTCGTCTTTTTCATCATGCGTTCAATGGGCGAAATGTTGTTCCTCGAaccggt                                                                               <  1:6256064/93‑1 (MQ=255)
           gggccATCCGTATTGTTGGCCTATATCATCGCCGGGCTGTTCGTCTTTTTCATCATGCGTTCAATGGGCGAAATGTTGTTCCTCGAaccggtt                                                                              >  2:5163120/1‑93 (MQ=255)
            ggccATCCGTATTGTTGGCCTATATCATCGCCGGGCTGTTCGTCTTTTTCATCATGCGTTCAATGGGCGAAATGTTGTTCCTCGAaccggtta                                                                             <  1:3948324/93‑1 (MQ=255)
            ggccATCCGTATTGTTGGCCTATATCATCGCCGGGCTGTTCGTCTTTTTCATCATGCGTTCAATGGGCGAAATGTTGTTCCTCGAaccggtta                                                                             >  1:3316034/1‑93 (MQ=255)
            ggccATCCGTATTGTTGGCCTATATCATCGCCGGGCTGTTCGTCTTTTTCATCATGCGTTCAATGGGCGAAATGTTGTTCCTCGAaccggtta                                                                             <  1:2840467/93‑1 (MQ=255)
            ggccATCCGTATTGTTGGCCTATATCATCGCCGGGCTGTTCGTCTTTTTCATCATGCGTTCAATGGGCGAAATGTTGTTCCTCGAaccggtta                                                                             <  2:457473/93‑1 (MQ=255)
              ccATCCGTATTGTTGGCCTATATCATCGCCGGGCTGTTCGTCTTTTTCATCATGCGTTCAATGGGCGAAATGTTGTTCCTCGAaccggttacc                                                                           <  2:644766/93‑1 (MQ=255)
              ccATCCGTATTGTTGGCCTATATCATCGCCGGGCTGTTCGTCTTTTTCATCATGCGTTCAATGGGCGAAATGTTGTTCCTCGAaccggttacc                                                                           <  2:876266/93‑1 (MQ=255)
              ccATCCGTATTGTTGGCCTATATCATCGCCGGGCTGTTCGTCTTTTTCATCATGCGTTCAATGGGCGAAATGTTGTTCCTCGAaccggttacc                                                                           >  1:1866813/1‑93 (MQ=255)
               cATCCGTATTGTTGGCCTATATCATCGCCGGGCTGTTCGTCTTTTTCATCATGCGTTCAATGGGCGAAATGTTGTTCCTCGAaccggttaccg                                                                          <  2:3812756/93‑1 (MQ=255)
               cATCCGTATTGTTGGCATATATCATCGCCGGGCTGTTCGTCTTTTTCATCATGCGTTCAATGGGCGAAATGTTGTTCCTCGAaccggttaccg                                                                          <  1:2754686/93‑1 (MQ=255)
                 tCCGTATTGTTGGCCTATATCATCGCCGGGCTGTTCGTCTTTTTCATCATGCGTTCAATGGGCGAAATGTTGTTCCTCGAaccggttaccggt                                                                        <  2:5723643/93‑1 (MQ=255)
                  ccGTATTGTTGGCCTATATCATCGCCGGGCTGTTCGTCTTTTTCATCATGCGTTCAATGGGCGAAATGTTGTTCCTCGAaccggttaccggtt                                                                       <  2:5358723/93‑1 (MQ=255)
                   cGTATTGTTGGCCTATATCATCGCCGGGCTGTTCGTCTTTTTCATCATGCGTTCAATGGGCGGAATGTTGTTCCTCGAACCGGTTACCGgttc                                                                      >  1:234362/1‑93 (MQ=255)
                   cGTATTGTTGGCCTATATCATCGCCGGGCTGTTCGTCTTTTTCATCATGCGTTCAATGGGCGAAATGTTGTTCCTCGAACCGGTTACCGgttc                                                                      <  1:1073529/93‑1 (MQ=255)
                   cGTATTGTTGGCCTATATCATCGCCGGGCTGTTCGTCTTTTTCATCATGCGTTCAATGGGCGAAATGTTGTTCCTCGAACCGGTTACCGgttc                                                                      >  1:1357837/1‑93 (MQ=255)
                     tATTGTTGGCCTATATCATCGCCGGGCTGTTCGTCTTTTTCATCATGCGTTCAATGGGCGAAATGTTGTTCCTCGAACCGGTTACCGgttcgt                                                                    >  2:3919527/1‑93 (MQ=255)
                      aTTGTTGGCCTATATCATCGCCGGGCTGTTCGTCTTTTTCATCATGCGTTCAATGGGCGAAATGTTGTTCCTCGAACCGGTTACCGgttcgtt                                                                   >  2:2422055/1‑93 (MQ=255)
                        ttttgGCCTATATCAGCGCCGGGCTGTTCGTCTTTTTCATCATGCGTTCAATGGGCGAAATGTTGTTCCTCGAACCGGTTACCGGttcgttcg                                                                 <  1:1462139/91‑1 (MQ=255)
                         gttgGCCTATATCATCGCCGGGCTGTTCGTCTTTTTCATCATGCGTTCAATGGGCGAAATGTTGTTCCTCGAACCGGTTACCGGTTCGTTCGc                                                                >  2:2748663/1‑93 (MQ=255)
                         gttgGCCTATATCATCGCCGGGCTGTTCGTCTTTTTCATCATGCGTTCAATGGGCGAAATGTTGTTCCTCGAACCGGTTACAGGTTCGTTCGc                                                                >  1:539512/1‑93 (MQ=255)
                          ttgGCCTATATCATCGCCGGGCTGTTCGTCTTTTTCATCATGCGTTCAATGGGCGAAATGTTGTTCCTCGAACCGGTTACCGGTTCGTTCGcc                                                               <  2:4049069/93‑1 (MQ=255)
                           tgGCCTATATCATCGCCGGGCTGTTCGTCTTTTTCATCATGCGTTCAATGGGCGAAATGTTGTTCCTCGAACCGGTTACCGGTTCGTTCGCCg                                                              <  2:2885015/93‑1 (MQ=255)
                            ggCCTATATCATCGCCGGGCTGTTCGTCTTTTTCATCATGCGTTCAATGGGCGAAATGTTGTTCCTCGAACCGGTTACCGGTTCGTTCGCCGt                                                             <  2:6216122/93‑1 (MQ=255)
                             gCCTATATCATCGCCGGTCTGTTCGTCTTTTTCATCATGCGTTCAATGGGCGAAATGTTGTTCCTCGAACCGGTTACCGGTTCGTTCGCCGtt                                                            <  1:5977698/93‑1 (MQ=255)
                             gCCTATATCATCGCCGGGCTGTTCGTCTTTTTCATCATGCGTTCAATGGGCGAAATGTTGTTCCTCGAACCGGTTACCGGTTCGTTCGCCGtt                                                            >  2:2641517/1‑93 (MQ=255)
                             gCCTATATCATCGCCGGGCTGTTCGTCTTTTTCATCATGCGTTCAATGGGCGAAATGTTGTTCCTCGAACCGGTTACCGGTTCGTTCGCCGtt                                                            >  1:3738640/1‑93 (MQ=255)
                               cTATATCATCGCCGGGCTGTTCGTCTTTTTCATCATGCGTTCAATGGGCGAAATGTTGTTCCTCGAACCGGTTACCGGTTCGTTCGCCGtttt                                                          >  2:2585368/1‑92 (MQ=255)
                               cTATATCATCGCCGGGCTGTTCGTCTTTTTCATCATGCGTTCAATGGGCGAAATGTTGTTCCTCGAACCGGTTACCGGTTCGTTCGCCGTTTa                                                          <  2:3439528/93‑1 (MQ=255)
                               cTATATCATCGCCGGGCTGTTCGTCTTTTTCATCATGCGTTCAATGGGCGAAATGTTGTTCCTCGAACCGGTTACCGGTTCGTTCGCCGTTTa                                                          <  2:3192294/93‑1 (MQ=255)
                                tataTCATCGCCGGGCTGTTCGTCTTTTTCATCATGCGTTCAATGGGCGAAATGTTGTTCCTCGAACCGGTTACCGGTTCGTTCGCCGTTTAt                                                         <  1:4271675/93‑1 (MQ=255)
                                 atatCATCGCCGGGCTGTTCGTCTTTTTCATCATGCGTTCAATGGGCGAAATGTTGTTCCTCGAACCGGTTACCGGTTCGTTCGCCGTTTATg                                                        <  2:1524749/93‑1 (MQ=255)
                                  tatCATCGCCGGGCTGTTCGTCTTTTTCATCATGCGTTCAATGGGCGAAATGTTGTTCCTCGAACCGGTTACCGGTTCGTTCGCCGTTTATgc                                                       <  1:1720174/93‑1 (MQ=255)
                                   atcatcGCCTGGCTGTTCGTCTTTTTCATCATGCGTTCAATGGGCGAAATGTTGTTCCTCGAACCGGTTCCCGGTTCGTTCGCCGTTTATgcg                                                      <  1:116892/93‑1 (MQ=255)
                                   atcatcGCCGGGCTGTTCGTCTTTTTCATCATGCGTTCAATGGGCGAAATGTTGTTCCTCGAACCGGTTACCGGTTCGTTCGCCGTTTATgcg                                                      >  2:3059015/1‑93 (MQ=255)
                                     catcGCCGGGCTGTTCGTCTTTTTCATCATGCGTTCAATGGGCGAAATGTTGTTCCTCGAACCGGTTACCGGTTCGTTCGCCGTTTATGCGCa                                                    >  1:4498090/1‑93 (MQ=255)
                                     catcGCCGGGCTGTTCGTCTTTTTCATCATGCGTTCAATGGGCGAAATGTTGTTCCTCGAACCGGTTACCGGTTCGTTCGCCGTTTATGCGCa                                                    <  1:3855923/93‑1 (MQ=255)
                                     catcGCCGGGCTGTTCGTCTTTTTCATCATGCGTTCAATGGGCGAAATGTTGTTCCTCGAACCGGTTACCGGTTCGTTCGCCGTTTATGCGCa                                                    >  2:512150/1‑93 (MQ=255)
                                      atcGCCGGGCTGTTCGTCTTTTTCATCATGCGTTCAATGGGCGAAATGTTGTTCCTCGAACCGGTTACCGGTTCGTTCGCCGTTTATGCGCAt                                                   >  1:6169432/1‑93 (MQ=255)
                                      atcGCCGGGCTGTTCGTCTTTTTCATCATGCGTTCAATGGGCGAAATGTCGTTCCTCGAACCGGTTACCGGTTCGTTCGCCGTTTATGAGCAt                                                   >  2:2332774/1‑93 (MQ=255)
                                       tcGCCGGGCTGTTCGTCTTTTTCATCATGCGTTCAATGGGCGAAATGTTGTTCCTCGAACCGGTTACCGGTTCGTTCGCCGTTTATGCGCATc                                                  >  1:5424517/1‑93 (MQ=255)
                                         gCCGGGCTGTTCGTCTTTTTCATCATGCGTTCAATGGGCGAAATGTTGTTCCTCGAACCGGTTACCGGTTCGTTCGCCGTTTATGCGCATCGt                                                <  1:5799375/93‑1 (MQ=255)
                                             ggCTGTTCGTCTTTTTCATCATGCGTTCAATGGGCGAAATGTTGTTCCTCGAACCGGTTACCGGTTCGTTCGCCGTTTATGCGCATCGTtata                                            <  2:6257153/93‑1 (MQ=255)
                                             ggCTGTTCGTCTTTTTCATCATGCGTTCAATGGGCGAAATGTTGTTCCTCGAACCGGTTACCGGTTCGTTCGCCGTTTATGCGCATCGTtata                                            >  1:1015761/1‑93 (MQ=255)
                                             ggCTGTTCGTCTTTTTCATCATGCGTTCAATGGGCGAAATGTTGTTCCTCGAACCGGTTACCGGTTCGTTCGCCGTTTATGCGCATCGTtata                                            <  1:3276273/93‑1 (MQ=255)
                                              gCTGTTCGTCTTTTTCATCATGCGTTCAATGGGCGAAATGTTGTTCCTCGAACCGGTTACCGGTTCGTTCGCCGTTTATGCGCATCGTTatat                                           >  2:4942619/1‑93 (MQ=255)
                                              gCTGTTCGTCTTTTTCATCATGCGTTCAATGGGCGAAATGTTGTTCCTCGAACCGGTTACCGGTTCGTTCGCCGTTTATGCGCATCGTTatat                                           >  1:2047228/1‑93 (MQ=255)
                                               cTGTTCGTCTTTTTCATCATGCGTTCAATGGGCGAAATGTTGTTCCTCGAATCGGTTACCGGTTCGTTCGCCGTTTATGCGCATCGTTATATg                                          >  1:5326022/1‑93 (MQ=255)
                                               cTGTTCGTCTTTTTCATCATGCGTTCAATGGGCGAAATGTTGTTCCTCGAACCGGTTACCGGTTCGTTCGCCGTTTATGCGCATCGTTATATg                                          >  2:3245555/1‑93 (MQ=255)
                                                  ttCGTCTTTTTCATCATGCGTTCAATGGGCGAAATGTTGTTCCTCGAACCGGTTACCGGTTCGTTCGCCGTTTATGCGCATCGTTATATGAGc                                       >  2:2009354/1‑93 (MQ=255)
                                                  ttCGTCTTTTTCATCATGCGTTCAATGGGCGAAATGTTGTTCCTCGAACCGGTTACCGGTTCGTTCGCCGTTTATGCGCATCGTTATATGAGc                                       >  1:5645207/1‑93 (MQ=255)
                                                   tCGTCTTTTTCATCATGCGTTCAATGGGCGAAATGTTGTTCCTCGAACCGGTTACCGGTTCGTTCGCCGTTTATGCGCATCGTTATATGAGcc                                      >  1:4743532/1‑93 (MQ=255)
                                                    cGTCTTTTTCATCATGCGTTCAATGGGCGAAATGTTGTTCCTCGAACCGGTTACCGGTTCGTTCGCCGTTTATGCGCATCGTTATATGAGccc                                     >  2:6087612/1‑93 (MQ=255)
                                                    cGTCTTTTTCATCATGCGTTCAATGGGCGAAATGTTGTTCCTCGAACCGGTTACCGGTTCGTTCGCCGTTTATGCGCATCGTTATATGAGccc                                     <  2:4437516/93‑1 (MQ=255)
                                                         ttttCATCATGCGTTCAATGGGCGAAATGTTGTTCCTCGAACCGGTTACCGGTTCGTTCGCCGTTTATGCGCATCGTTATATGAGCCCGTTCt                                >  1:4068450/1‑93 (MQ=255)
                                                           ttCATCATGCGTTCAATGGGCGAAATGTTGTTCCTCGAACCGGTTACCGGTTCGTTCGCCGTTTATGCGCATCGTTATATGAGCCCGTTCttt                              <  2:2140494/93‑1 (MQ=255)
                                                            tcatcaTGCGTTCAATGGGCGAAATGTTGTTCCTCGAACCGGTTACCGGTTCGTTCGCCGTTTATGCGCATCGTTATATGAGCCCGTTCTTTg                             <  1:5600006/93‑1 (MQ=255)
                                                            tcatcaTGCGTTCAATGGGCGAAATGTTGTTCCTCGAACCGGTTACCGGTTCGTTCGCCGTTTATGCGCATCGTTATATGAGCCCGTTCTTTg                             <  2:5059363/93‑1 (MQ=255)
                                                              atcatGCGTTCAATGGGCGAAATGTTGTTCCTCGAACCGGTTACCGGTTCGTTCGCCGTTTATGCGCATCGTTATATGAGCCCGTTCTTTGGc                           <  1:4635960/93‑1 (MQ=255)
                                                              atcatGCGTTCAATGGGCGAAATGTTGTTCCTCGAACCGGTTACCGGTTCGTTCGCCGTTTATGCGCATCGTTATATGAGCCCGTTCTTTGGc                           <  1:938631/93‑1 (MQ=255)
                                                               tcatGCGTTCAATGGGCGAAATGTTGTTCCTCGAACCGGTTACCGGTTCGTTCGCCGTTTATGCGCATCGTTATATGAGCCCGTTCTTTGGCt                          >  1:3551173/1‑93 (MQ=255)
                                                               tcatGCGTTCAATGGGCGAAATGTTGTTCCTCGAACCGGTTACCGGTTCGTTCGCCGTTTATGCGCATCGTTATATGAGCCCGTTCTTTGGCt                          >  2:1942241/1‑93 (MQ=255)
                                                                catGCGTTCAATGGGCGAAATGTTGTTCCTCGAACCGGTTACCGGTTCGTTCGCCGTTTATGCGCATCGTTATATGAGCCCGTTCTTTGGCTa                         <  1:2252743/93‑1 (MQ=255)
                                                                 atGCGTTCAATGGGCGAAATGTTGTTCCTCGAACCGGTTACCGGTTCGTTCGCCGTTTATGCGCATCGTTATATGAGCCCGTTCTTTGGCTAt                        <  2:1422040/93‑1 (MQ=255)
                                                                 atGCGTTCAATGGGCGAAATGTTGTTCCTCGAACCGGTTACCGGTTCGTTCGCCGTTTATGCGCATCGTTATATGAGCCCGTTCTTTGGCTAt                        >  2:4210337/1‑93 (MQ=255)
                                                                  tGCGTTCAATGGGCGAAATGTTGTTCCTCGAACCGGTTACCGGTTCGTTCGCCGTTTATGCGCATCGTTATATGAGCCCGTTCTTTGGCTAtc                       >  1:5065249/1‑93 (MQ=255)
                                                                   gCGTTCAATGGGCGAAATGTTGTTCCTCGAACCGGTTACCGGTTCGTTCGCCGTTTATGCGCATCGTTATATGAGCCCGTTCTTTGGCTAtct                      <  1:4720458/93‑1 (MQ=255)
                                                                   gCGTTCAATGGGCGAAATGTTGTTCCTCGAACCGGTTACCGGTTCGTTCGCCGTTTATGCGCATCGTTATATGAGCCCGTTCTTTGGCTAtct                      <  2:5894396/93‑1 (MQ=255)
                                                                     gTTCAATGGGCGAAATGTTGTTCCTCGAACCGGTTACCGGTTCGTTCGCCGTTTATGCGCATCGTTATATGAGCCCGTTCTTTGGCTATCTCa                    >  1:161477/1‑93 (MQ=255)
                                                                      ttCAATGGGCGAGATGTTGTTCCTCGAACCGGTTACCGGTTCGTTCGCCGTTTATGCGCATCGTTATATGAGCCCGTTCTTTGGCTATCTCAc                   >  1:3486207/1‑93 (MQ=255)
                                                                      ttCAATGGGCGAAATGTTGTTCCTCGAACCGGTTACCGGTTCGTTCGCCGTTTATGCGCATCGTTATATGAGCCCGTTCTTTGGCTATCTCAc                   >  2:5266526/1‑93 (MQ=255)
                                                                      ttCAATGGGCGAAATGTTGTTCCTCGAACCGGTTACCGGTTCGTTCGCCGTTTATGCGCATCGTTATATGAGCCCGTTCTTTGGCTATCTCAc                   >  2:5486071/1‑93 (MQ=255)
                                                                          aTGGGCGAAATGTTGTTCCTCGAACCGGTTACCGGTTCGTTCGCCGTTTATGCGCATCGTTATATTAGCCCGTTCTTTGCATATATCACCTcc               >  2:4667842/1‑93 (MQ=255)
                                                                           tGGGCGAAATGTTGTTCCTCGAACCGGTTACCGGTTCGTTCGCCGTTTATGCGCATCGTTATATGAGCCCGTTCTTTGGCTATCTCACCGCCt              >  2:6044916/1‑93 (MQ=255)
                                                                           tGGGCGAAATGTTGTTCCTCGAACCGGTTACCGGTTCGTTCGCCGTTTATGCGCATCGTTATATGAGCCCGTTCTTTGGCTATCTCACCGCCt              <  1:6206/93‑1 (MQ=255)
                                                                            gggCGAAATGTTGTTCCTCGAACCGGTTACCGGTTCGTTTGCCGTTTATGCGCATCGTTATATGAGCCCGTTCTTTGGCTATCTCACCGCCTg             <  1:801416/93‑1 (MQ=255)
                                                                             ggCGAAATGTTGTTCCTCGAACCGGTTACCGGTTCGTTCGCCGTTTATGCGCATCGTTATATGAGCCCGTTCTTTGGCTATCTCACCGCCTgg            <  2:3519089/93‑1 (MQ=255)
                                                                              gCGAAATGTTGTTCCTCGAACCGGTTACCGGTTCGTTCGCCGTTTATGCGCATCGTTATATGAGCCCGTTCTTTGGCTATCTCACCGCCTGGt           >  2:3953496/1‑93 (MQ=255)
                                                                               cGAAATGTTGTTCCTCGAACCGGTTACCGGTTCGTTCGCCGTTTATGCGCATCGTTATATGAGCCCGTTCTTTGGCTATCTCACCGCCTGGTc          <  1:3434167/93‑1 (MQ=255)
                                                                                gAAATGTTGTTCCTCGAACCGGTTACCGGTTCGTTCGCCGTTTATGCGCATCGTTATATGAGCCCGTTCTTTGGCTATCTCACCGCCTGGTCt         <  2:174760/93‑1 (MQ=255)
                                                                                   aTGTTGTTCCTCGAACCGGTTACCGGTTCGTTCGCCGTTTATGCGCATCGTTATATGAGCCCGTTCTTTGGCTATCTCACCGCCTGGTCTTAc      >  1:4406727/1‑93 (MQ=255)
                                                                                   aTGTTGTTCCTCGAACCGGTTACCGGTTCGTTCGCCGTTTATGCGCATCGTTATATGAGCCCGTTCTTTGGCTATCTCACCGCCTGGTCTTAc      <  2:6341669/93‑1 (MQ=255)
                                                                                    tgttgtTCCTCGAACCGGTTACCGGTTCGTTCGCCGTTTATGCGCATCGTTATATGAGCCCGTTCTTTGGCTATCTCACCGCCTGGTCTTACt     <  1:1772936/93‑1 (MQ=255)
                                                                                      ttgtTCCTCGAACCGGTTACCGGTTCGTTCGCCGTTTATGCGCATCGTTATATGAGCCCGTTCTTTGGCTATCTCACCGCCTGGTCTTACTgg   <  1:5718612/93‑1 (MQ=255)
                                                                                      ttgtTCCTCGAACCGGTTACCGGTTCGTTCGCCGTTTATGCGCATCGTTATATGAGCCCGTTCTTTGGCTATCTCACCGCCTGGTCTTACTgg   >  2:4312066/1‑93 (MQ=255)
                                                                                       tgtTCCTCGAACCGGTTACCGGTTCGTTCGCCGTTTATGCGCATCGTTATATGAGCCCGTTCTTTGGCTATCTCACCGCCTGGTCTTACTGGt  <  2:3405373/93‑1 (MQ=255)
                                                                                          |                                                                                         
TGAAATGGGCCGGGCCATCCGTATTGTTGGCCTATATCATCGCCGGGCTGTTCGTCTTTTTCATCATGCGTTCAATGGGCGAAATGTTGTCCCTCGAACCGGTTACCGGTTCGTTCGCCGTTTATGCGCATCGTTATATGAGCCCGTTCTTTGGCTATCTCACCGCCTGGTCTTACTGGT  >  NC_000913/3978612‑3978791

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
CCCTGAAATGGGCCGGGCCATCCGTATTGTTGGCCTATATCATCGCCGGGCTGTTCGTCTTTTTCATCATGCGTTCAATGGGCGAAATGTTGTCCCTCGAACCGGTTACCGGTTCGTTCGCCGTTTATGCGCATCGTTATATGAGCCCGTTCTTTGGCTATCTCACCGCCTGGTCTTACTGGTTTAT  >  NC_000913/3978609‑3978795
                                                                                             |                                                                                             
CCCTGAAATGGGCCGGGCCATCCGTATTGTTGGCCTATATCATCGCCGGGCTGTTCGTCTTTTTCATCATGCGTTCAATGGGCGAAATGTTGTT                                                                                               <  SRR4302537.5882742/94‑1 (MQ=60)
CCCTGAAATGGGCCGGGCCATCCGTATTGTTGGCCTATATCATCGCCGGGCTGTTCGTCTTTTTCATCATGCGTTCAATGGGCGAAATGTTGTT                                                                                               <  SRR4302537.4462312/94‑1 (MQ=60)
CCCTGAAATGGGCCGGGCCATCCGTATTGTTGGCCTATATCATCGCCGGGCTGTTCGTCTTTTTCATCATGCGTTCAATGGGCGAAATGTTGTT                                                                                               >  SRR4302537.1840955/1‑94 (MQ=60)
CCCTGAAATGGGCCGGGCCATCCGTATTGTTGGCCTATATCATCGCCGGGCTGTTCGTCTTTTTCATCATGCGTTCAATGGGCGAAATGTTGTT                                                                                               <  SRR4302537.6175362/94‑1 (MQ=60)
 CCTGAAATGGGCCGGGCCATCCGTATTGTTGGCCTATATCATCGCCGGGCTGTTCGTCTTTTTCATCATGCGTTCAATGGGCGAAATGTTGTTC                                                                                              <  SRR4302537.340877/94‑1 (MQ=60)
   TGAAATGGGCCGGGCCATCCGTATTGTTGGCCTATATCATCGCCGGGCTGTTCGTCTTTTTCATCATGCGTTCAATGGGCGAAATGTTGTTCCT                                                                                            >  SRR4302537.1966578/1‑94 (MQ=60)
   TGAAATGGGCCGGGCCATCCGTATTGTTGGCCTATATCATCGCCGGGCTGTTCGTCTTTTTCATCATGCGTTCAATGGGCGAAATGTTGTTCCT                                                                                            >  SRR4302537.766067/1‑94 (MQ=60)
     AAATGGGCCGGGCCATCCGTATTGTTGGCCTATATCATCGCCGGGCTGTTCGTCTTTTTCATCATGCGTTCAATGGGCGAAATGTTGTTCCTCG                                                                                          <  SRR4302537.3163148/94‑1 (MQ=60)
      AATGGGCCGGGCCATCCGTATTGTTGGCCTATATCATCGCCGGGCTGTTCGTCTTTTTCATCATGCGTTCAATGGGCGAAATGTTGTTCCTCGA                                                                                         >  SRR4302537.244495/1‑94 (MQ=60)
       ATGGGCCGGGCCATCCGTATTGTTGGCCTATATCATCGCCGGGCTGTTCGTCTTTTTCATCATGCGTTCAATGGGCGAAATGTTGTTCCTCGAA                                                                                        >  SRR4302537.2100806/1‑94 (MQ=60)
       ATGGGCCGGGCCATCCGTATTGTTGGCCTATATCATCGCCGGGCTGTTCGTCTTTTTCATCATGCGTTCAATGGGCGAAATGTTGTTCCTCGAA                                                                                        <  SRR4302537.3375220/94‑1 (MQ=60)
        TGGGCCGGGCCATCCGTATTGTTGGCCTATATCATCGCCGGGCTGTTCGTCTTTTTCATCATGCGTTCAATGGGCGAAATGTTGTTCCTCGAAC                                                                                       <  SRR4302537.3137519/94‑1 (MQ=60)
         GGGCCGGGCCATCCGTATTGTTGGCCTATATCATCGCCGGGCTGTTCGTCTTTTTCATCATGCGTTCAATGGGCGAAATGTTGTTCCTCGAACC                                                                                      >  SRR4302537.2282192/1‑94 (MQ=60)
         GGGCCGGGCCATCCGTATTGTTGGCCTATATCATCGCCGGGCTGTTCGTCTTTTTCATCATGCGTTCAATGGGCGAAATGTTGTTCCTCGAACC                                                                                      <  SRR4302537.3029167/94‑1 (MQ=60)
          GGCCGGGCCATCCGTATTGTTGGCCTATATCATCGCCGGGCTGTTCGTCTTTTTCATCATGCGTTCAATGGGCGAAATGTTGTTCCTCGAACCG                                                                                     >  SRR4302537.3168083/1‑94 (MQ=60)
          GGCCGGGCCATCCGTATTGTTGGCCTAAATCCTCGCCAGGCTGTTCATCTTTTTCATCATGCGTTCAATGGGCGAAATGTTGTTCCTCGAACCG                                                                                     <  SRR4302537.6514985/94‑1 (MQ=60)
          GGCCGGGCCATCCGTATTGTTGGCCTAAATCATCGCCGGGCTGTTCGTCTTTTTCATCATGCGTTCAATGGGCGAAATGTTGTTCCTCGAACCG                                                                                     <  SRR4302537.4403302/94‑1 (MQ=60)
            CCGGGCCATCCGTATTGTTGGCCTATATCATCGCCGGGCTGTTCGTCTTTTTCATCATGCGTTCAATGGGCGAAATGTTGTTCCTCGAACCGGT                                                                                   >  SRR4302537.3187039/1‑94 (MQ=60)
             CGGGCCATCCGTATTGTTGGCCTATATCATCGCCGGGCTGTTCGTCTTTTTCATCATGCGTTCAATGGGCGAAATGTTGTTCCTCGAACCGGTT                                                                                  <  SRR4302537.6299035/94‑1 (MQ=60)
             CGGGCCATCCGTATTGTTGGCCTATATCATCGCCGGGCTGTTCGTCTTTTTCATCATGCGTTCAATGGGCGAAATGTTGTTCCTCGAACCGGTT                                                                                  >  SRR4302537.1084536/1‑94 (MQ=60)
              GGGCCATCCGTATTGTTGGCCTATATCATCGCCGGGCTGTTCGTCTTTTTCATCATGCGTTCAATGGGCGAAATGTTGTTCCTCGAACCGGTTA                                                                                 >  SRR4302537.3339490/1‑94 (MQ=60)
              GGGCCATCCGTATTGTTGGCCTATATCATCGCCGGGCTGTTCGTCTTTTTCATCATGCGTTCAATGGGCGAAATGTTGTTCCTCGAACCGGTTA                                                                                 <  SRR4302537.5119787/94‑1 (MQ=60)
               GGCCATCCGTATTGTTGGCCTATATCATCGCCGGGCTGTTCGTCTTTTTCATCATGCGTTCAATGGGCGAAATGTTGTTCCTCGAACCGGTTAC                                                                                <  SRR4302537.2860346/94‑1 (MQ=60)
               GGCCATCCGTATTGTTGGCCTATATCATCGCCGGGCTGTTCGTCTTTTTCATCATGCGTTCAATGGGCGAAATGTTGTTCCTCGAACCGGTTAC                                                                                <  SRR4302537.3975937/94‑1 (MQ=60)
                GCCATCCGTATTGTTGGCCTATATCATCGCCGGGCTGTTCGTCTTTTTCATCATGCGTTCAATGGGCGAAATGTTGTTCCTCGAACCGGTTACC                                                                               >  SRR4302537.1879808/1‑94 (MQ=60)
                 CCATCCGTATTGTTGGCCTATATCATCGCCGGGCTGTTCGTCTTTTTCATCATGCGTTCAATGGGCGAAATGTTGTTCCTCGAACCGGTTACCG                                                                              <  SRR4302537.34942/94‑1 (MQ=60)
                  CATCCGTATTGTTGGCCTATATCATCGCCGGGCTGTTCGTCTTTTTCATCATGCGTTCAATGGGCGAAATGTTGTTCCTCGAACCGGTTACCGG                                                                             <  SRR4302537.3305289/94‑1 (MQ=60)
                  CATCCGTATTGTTGGCCTATATCATCGCCGGGCTGTTCGGCTTTTTCATCATGCGTTCAATGGGCGAAATGTTGTTCCTCGAACCGGTTACCGG                                                                             <  SRR4302537.1447313/94‑1 (MQ=60)
                  CATCCGTATTGTTGGCATATATCATCGCCGGGCTGTTCGTCTTTTTCATCATGCGTTCAATGGGCGAAATGTTGTTCCTCGAACCGGTTACCGG                                                                             <  SRR4302537.2773917/94‑1 (MQ=60)
                   ATCCGTATTGTTGGCCTATATCATCGCCGGGCTGTTCGTCTTTTTCATCATGCGTTCAATGGGCGACATGTTGTTCCTCGTACCGGTTACCGGT                                                                            >  SRR4302537.6047863/1‑94 (MQ=60)
                   ATCCGTATTGTTGGCCTATATCATCGCCGGGCTGTTCGTCTTTTTCATCATGCGTTCAATGGGCGAAATGTTGTTCCTCGAACCGGTTACCGGT                                                                            >  SRR4302537.6029636/1‑94 (MQ=60)
                   ATCCGTATTGTTGGCCTATATCATCGCCGGGCTGTTCGTCTTTTTCATCATGCGTTCAATGGGCGAAATGTTGTTCCTCGAACCGGTTACCGGT                                                                            >  SRR4302537.842252/1‑94 (MQ=60)
                    TCCGTATTGTTGGCCTATATCATCGCCGGGCTGTTCGTCTTTTTCATCATGCGTTCAATGGGCGAAATGTTGTTCCTCGAACCGGTTACCGGTT                                                                           <  SRR4302537.4403825/94‑1 (MQ=60)
                     CCGTATTGTTGGCCTATATCATCGCCGGGCTGTTCGTCTTTTTCATCATGCGTTCAATGGGCGGAATGTTGTTCCTCGAACCGGTTACCGGTTC                                                                          >  SRR4302537.236019/1‑94 (MQ=60)
                     CCGTATTGTTGGCCTATATCATCGCCGGGCTGTTCGTCTTTTTCATCATGCGTTCAATGGGCGAAATGTTGTTCCTCGAACCGGTTACCGGTTC                                                                          >  SRR4302537.1367292/1‑94 (MQ=60)
                     CCGTATTGTTGGCCTATATCATCGCCGGGCTGTGCGTCTTTTTCATCATGCGTTCAATGGGCGAAATGTTGTTCCTCGAACCGGTTACCGGTTC                                                                          <  SRR4302537.6215432/94‑1 (MQ=60)
                      CGTATTGTTGGCCTATATCATCGCCGGGCTGTTCGTCTTTTTCATCATGCGTTCAATGGGCGAAATGTTGTTCCTCGAACCGGTTACCGGTTCG                                                                         <  SRR4302537.1080924/94‑1 (MQ=60)
                      CGTATTGTTGGCCTATATCATCGCCGGGCTGTTCGTCTTTTTCATCATGCGTTCAATGGGCGAAATGTTGTTCCTCGAACCGGTTACCGGTTCG                                                                         <  SRR4302537.2365652/94‑1 (MQ=60)
                      CGTATTGTTGGCCTATATCATCGCCGGGCTGTTCGTCTTTTTCATCATGCGTTCAATGGGCGAAATGTTGTTCCTCGAACCGGTTACCGGTTCG                                                                         >  SRR4302537.3552965/1‑94 (MQ=60)
                      CGTATTGTTGGCCTATATCATCGCCGGGCTGTTCGTCTTTTTCATCATGCGTTCAATGGGCGAAATGTTGTTCCTCGAACCGGTTACCGGTTCG                                                                         >  SRR4302537.380609/1‑94 (MQ=60)
                      CGTATTGTTGGCCTATATCATCGCCGGGCTGTTCGTCTTTTTCATCATGCGTTCAATGGGCGAAATGTTGTTCCTCGAACCGGTTACCGGTTCG                                                                         <  SRR4302537.6257315/94‑1 (MQ=60)
                      CGTATTGTTGGCCTATATCATCGCCGGGCTGTTCGTCTTTTTCATCATGCGTTCAATGGGCGAAATGTTGTTCCTCGAACCGGTTACCGGTTCG                                                                         >  SRR4302537.4761128/1‑94 (MQ=60)
                       CTATTGTTGGCCTATATCATCGCCGGGCTGTTCGTCTTTTTCATCATGCGTTCAATGGGCGAAATGTTGTTCCTCGAACCGGTTACCGGTTCGT                                                                        <  SRR4302537.4810418/94‑1 (MQ=60)
                        TATTGTTGGCCTATATCATCGCCGGGCTGTTCGTCTTTTTCATCATGCGTTCAATGGGCGAAATGTTGTTCCTCGAACCGGTTACCGGTTCGTT                                                                       <  SRR4302537.3268638/94‑1 (MQ=60)
                         ATTGTTGGCCTATATCATCGCCGGGCTGTTCGTCTTTTTCATCATGCGTTCAATGGGCGAAATGTTGTTCCTCGAACCGGTTACCGGTTCGTTC                                                                      <  SRR4302537.2342227/94‑1 (MQ=60)
                           TTTTGGCCTATATCAGCGCCGGGCTGTTCGTCTTTTTCATCATGCGTTCAATGGGCGAAATGTTGTTCCTCGAACCGGTTACCGGTTCGTTCGC                                                                    <  SRR4302537.1472310/94‑1 (MQ=60)
                           TGTTGGCCTATATCATCGCCGGGCTGTTCGTCTTTTTCATCATGCGTTCAATGGGCGAAATGTTGTTCCTCGAACCGGTTACCGGTTCGTTCGC                                                                    <  SRR4302537.3206466/94‑1 (MQ=60)
                           TGTTGGCCTATATCATCGCCGGGCTGTTCGTCTTTTTCATCATGCGTTCAATGGGCGAAATGTTGTTCCTCGAACCGGTTACCGGTTCGTTCGC                                                                    <  SRR4302537.5571027/94‑1 (MQ=60)
                           TGTTGGCCTATATCATCGCCGGGCTGTTCGTCTTTTTCATCATGCGTTCAATGGGCGAAATGTTGTTCCTCGAACCGGTTACCGGTTCGTTCGC                                                                    >  SRR4302537.5390296/1‑94 (MQ=60)
                           TGTTGGCCTATATCATCGCCGGGCTGTTCGTCTTTTTCATCATGCGTTCAATGGGCGAAATGTTGTTCCTCGAACCGGTTACCGGTTCGTTCGC                                                                    >  SRR4302537.543223/1‑94 (MQ=60)
                           TGTTGGCCTATATCATCGCCGGGCTGTTCGTCTTTTTCATCATGCGTTCAATGGGCGAAATGTTGTTCCTCGAACCGGTTACAGGTTCGTTCGC                                                                    >  SRR4302537.543222/1‑94 (MQ=60)
                              TGGCCTATATCATCGCCGGGCTGTTCGTCTTTTTCATCATGCGTTCAATGGGCGAAATGTTGTTCCTCGAACCGGTTACCGGTTCGTTCGCCGT                                                                 <  SRR4302537.2692652/94‑1 (MQ=60)
                               GGCCTATATCATCGCCGGGCTGTTCGTCTTTTTCATCATGCGTTCAATGGGCGAAATGTTGTTCCTCGAACCGGTTACCGGTTCGTTCGCCGTT                                                                >  SRR4302537.3449866/1‑94 (MQ=60)
                               GGCCTATATCATCGCCGGGCTGTTCGTCTTTTTCATCATGCGTTCAATGGGCGAAATGTTGTTCCTCGAACCGGTTACCGGTTCGTTCGCCGTT                                                                >  SRR4302537.39313/1‑94 (MQ=60)
                               GGCCTATATCATCGCCGGGCTGTTCGTCTTTTTCATCATGCGTTCAATGGGCGAAATGTTGTTCCTCGAACCGGTTACCGGTTCGTTCGCCGTT                                                                >  SRR4302537.3764896/1‑94 (MQ=60)
                                GCCTATATCATCGCCGGTCTGTTCGTCTTTTTCATCATGCGTTCAATGGGCGAAATGTTGTTCCTCGAACCGGTTACCGGTTCGTTCGCCGTTT                                                               <  SRR4302537.6018733/94‑1 (MQ=60)
                                GCCTATATCATCGCCGGGCTGTTCGTCTTTTTCATCATGCGTTCAATGGGCGAAATGTTGTTCCTCGAACCGGTTACCGGTTCGTTCGCCGTTT                                                               <  SRR4302537.1762982/94‑1 (MQ=60)
                                 CCTATATCATCGCCGGGCTGTTCGTCTTTTTCATCATGCGTTCAATGGGCGAAATGTTGTTCCTCGAACCGGTTACCGGTTCGTTCGCCGTTTA                                                              <  SRR4302537.318183/94‑1 (MQ=60)
                                  CTATACCATCCGCGGGCTGTTCGTCTTTTTCATCATGCCTTCAATGGGCGAAATGTTGTTCCTCGAACCGGTTACCGGTTCGTTCGCCGTTTAT                                                             <  SRR4302537.1563973/94‑1 (MQ=60)
                                   TATATCATCGCCGGGCTGTTCGTCTTTTTCATCATGCGTTCAATGGGCGAAATGTTGTTCCTCGAACCGGTTACCGGTTCGTTCGCCGTTTATG                                                            >  SRR4302537.4696438/1‑94 (MQ=60)
                                   TATATCATCGCCGGGCTGTTCGTCTTTTTCATCATGCGTTCAATGGGCGAAATGTTGTTCCTCGAACCGGTTACCGGTTCGTTCGCCGTTTATG                                                            <  SRR4302537.4663104/94‑1 (MQ=60)
                                   TATATCATCGCCGGGCTGTTCGTCTTTTTCATCATGCGTTCAATGGGCGAAATGTTGTTCCTCGAACCGGTTACCGGTTCGTTCGCCGTTTATG                                                            >  SRR4302537.6501924/1‑94 (MQ=60)
                                   TATATCATCGCCGGGCTGTTCGTCTTTTTCATCATGCGTTCAATGGGCGAAATGTTGTTCCTCGAACCGGTTACCGGTTCGTTCGCCGTTTATG                                                            <  SRR4302537.4301358/94‑1 (MQ=60)
                                    ATATCATCGGCGGGCTGTTCGTCTTTTTCATCATGCGTTCAATGGGCGAAATGTTGTTCCTCGAACCGGTTACCGGTTCGTTCGCCGTTTATGC                                                           >  SRR4302537.4548682/1‑94 (MQ=60)
                                     TATCATCGCCGGGCTGTTCGTCTTTTTCATCATGCGTTCAATGGGCGAAATGTTGTTCCTCGAACCGGTTACCGGTTCGTTCGCCGTTTATGCG                                                          <  SRR4302537.1732198/94‑1 (MQ=60)
                                      ATCATCGCCTGGCTGTTCGTCTTTTTCATCATGCGTTCAATGGGCGAAATGTTGTTCCTCGAACCGGTTCCCGGTTCGTTCGCCGTTTATGCGC                                                         <  SRR4302537.117737/94‑1 (MQ=60)
                                       TCATCGCCGGGCTGTTCGTCTTTTTCATCATGCGTTCAATGGGCGAAATGTTGTTCCTCGAACCGGTTACCGGTTCGTTCGCCGTTTATGCGCA                                                        >  SRR4302537.4529204/1‑94 (MQ=60)
                                        CATCGCCGGGCTGTTCGTCTTTTTCATCATGCGTTCAATGGGCGAAATGTTGTTCCTCGAACCGGTTACCGGTTCGTTCGCCGTTTATGCGCAT                                                       >  SRR4302537.6211916/1‑94 (MQ=60)
                                        CATCGCCGGGCTGTTCGTCTTTTTCATCATGCGTTCAATGGGCGAAATGTTGTTCCTCGAACCGGTTACCGGTTCGTTCGCCGTTTATGCGCAT                                                       <  SRR4302537.1955470/94‑1 (MQ=60)
                                        CATCGCCGGGCTGTTCGTCTTTTTCATCATGCGTTCAATGGGCGAAATGTTGTTCCTCGAACCGGTTACCGGTTCGTTCGCCGTTTATGCGCAT                                                       <  SRR4302537.3882912/94‑1 (MQ=60)
                                        CATCGCCGGGCTGTTCGTCTTTTTCATCATGCGTTCAATGGGCGAAATGTTGTTCCTCGAACCGGTTACCGGTTCGTTCGCCGTTTATGCGCAT                                                       >  SRR4302537.5678102/1‑94 (MQ=60)
                                         ATCGCCGGGCTGTTCGTCTTTTTCATCATGCGTTCAATGGGCGAAATGTTGTTCCTCGAACCGGTTACCGGTTCGTTCGCCGTTTATGCGCATC                                                      <  SRR4302537.5970787/94‑1 (MQ=60)
                                         ATCGCCGGGCTGTTCGTCTTTTTCATCATGCGTTCAATGGGCGAAATGTTGTTCCTCGAACCGGTTACCGGTTCGTTCGCCGTTTATGCGCATC                                                      >  SRR4302537.5461489/1‑94 (MQ=60)
                                           CGCCGGGCTGTTCGTCTTTTTCATCATGCGTTCAATGGGCGAAATGTTGTTCCTCGAACCGGTTACCGGTTCGTTCGCCGTTTATGCGCATCGT                                                    >  SRR4302537.1059442/1‑94 (MQ=60)
                                            GCCGGGCTGTTCGTCTTTTTCATCATGCGTTCAATGGGCGAAATGTTGTTCCTCGAACCGGTTACCGGTTCGTTCGCCGTTTATGCGCATCGTT                                                   <  SRR4302537.5839135/94‑1 (MQ=60)
                                            GCCGGGCTGTTCGTCTTTTTCATCATGCGTTCAATGGGCGAAATGTTGTTCCTCGAACCGGTTACCGGTTCGTTCGCCGTTTATGCGCATCGTT                                                   <  SRR4302537.2091379/94‑1 (MQ=60)
                                            GCCGGGCTGTTCGTCTTTTTCATCATGCGTTCAATGGGCGAAATGTTGTTCCTCGAACCGGTTACCGGTTCGTTCGCCGTTTATGCGCATCGTT                                                   >  SRR4302537.175997/1‑94 (MQ=60)
                                             CCGGGCTGTTCGTCTTTTTCATCATGCGTTCAATGGGCGAAATGTTGTTCCTCGAACCGGTTACCGGTTCGTTCGCCGTTTATGCGCATCGTTA                                                  >  SRR4302537.3972177/1‑94 (MQ=60)
                                              CGGGCTGTTCGTCTTTTTCATCATGCGTTCAATGGGCGAAATGTTGTTCCTCGAACCGGTTACCGGTTCGTTCGCCGTTTATGCGCATCGTTAT                                                 <  SRR4302537.4027964/94‑1 (MQ=60)
                                               GGGCTGTTCGTCTTTTTCATCATGCGTTCAATGGGCGAAATGTTGTTCCTCGAACCGGTTACCGGTTCGTTCGCCGTTTATGCGCATCGTTATA                                                >  SRR4302537.1022756/1‑94 (MQ=60)
                                                GGCTGTTCGTCTTTTTCATCATGCGTTCAATGGGCGAAATGTTGTTCCTCGAACCGGTTACCGGTTCGTTCGCCGTTTATGCGCATCGTTATAT                                               >  SRR4302537.364457/1‑94 (MQ=60)
                                                GGCTGTTCGTCTTTTTCATCATGCGTTCAATGGGCGAAATGTTGTTCCTCGAACCGGTTACCGGTTCGTTCGCCGTTTATGCGCATCGTTATAT                                               >  SRR4302537.2061582/1‑94 (MQ=60)
                                                GGCTGTTCGTCTTTTTCATCATGCGTTCAATGGGCGAAATGTTGTTCCTCGAACCGGTTACCGGTTCGTTCGCCGTTTATGCGCATCGTTATAT                                               <  SRR4302537.3299393/94‑1 (MQ=60)
                                                 GCTGTTCGTCTTTTTCATCATGCGTTCAATGGGCGAAATGTTGTTCCTCGAATCGGTTACCGGTTCGTTCGCCGTTTATGCGCATCGTTATATG                                              >  SRR4302537.5362566/1‑94 (MQ=60)
                                                   TGTTCGTCTTTTTCATCATGCGTTCAATGGGCGAAATGTTGTTCCTCGAACCGGTTACCGGTTCGTTCGCCGTTTATGCGCATCGTTATATGAG                                            >  SRR4302537.5701079/1‑94 (MQ=60)
                                                   TGTTCGTCTTTTTCATCATGCGTTCAATGGGCGAAATGTTGTTCCTCGAACCGGTTACCGGTTCGTTCGCCGTTTATGCGCATCGTTATATGAG                                            <  SRR4302537.1526547/94‑1 (MQ=60)
                                                   TGTTCGTCTTTTTCATCATGCGTTCAATGGGCGAAATGTTGTTCCTCGAACCGGTTACCGGTTCGTTCGCCGTTTATGCGCATCGTTATATGAG                                            >  SRR4302537.448579/1‑94 (MQ=60)
                                                    GTTCGTCTTTTTCATCATGCGTTCAATGGGCGAAATGTTGTTCCTCGAACCGGTTACCGGTTCGTTCGCCGTTTATGCGCATCGTTATATGAGC                                           >  SRR4302537.5683844/1‑94 (MQ=60)
                                                     TTCGTCTTTTTCATCATGCGTTCAATGGGCGAAATGTTGTTCCTCGAACCGGTTACCGGTTCGTTCGCCGTTTATGCGCATCGTTATATGAGCC                                          >  SRR4302537.4776312/1‑94 (MQ=60)
                                                     TTCGTCTTTTTCATCATGCGTTCAATGGGCGAAATGTTGTTCCTCGAACCGGTTACCGGTTCGTTCGCCGTTTATGCGCATCGTTATATGAGCC                                          >  SRR4302537.4728245/1‑94 (MQ=60)
                                                      TCGTCTTTTTCATCATGCGTTCAATGGGCGAAATGTTGTTCCTCGAACCGGTTACCGGTTCGTTCGCCGTTTATGCGCATCGTTATATGAGCCC                                         >  SRR4302537.3552506/1‑94 (MQ=60)
                                                       CGTCTTTTTCATCATGCGTTCAATGGGCGAAATGTTGTTCCTCGAACCGGTTACCGGTTCGTTCGCCGTTTATGCGCATCGTTATATGAGTCCG                                        >  SRR4302537.2388927/1‑94 (MQ=60)
                                                       CGTCTTTTTCATCATGCGTTCAATGGGCGAAATGTTGTTCCTCGAACCGGTTACCGGTTCGTTCGCCGTTTATGCGCATCGTTATATGAGCCCG                                        >  SRR4302537.1789668/1‑94 (MQ=60)
                                                       CGTCTTTTTCATCATGCGTTCAATGGGCGAAATGTTGTTCCTCGAACCGGCTACCGGTTCGTTCGCCGTTTATGCGCATCGTTATATGAGCCCG                                        <  SRR4302537.4194609/94‑1 (MQ=60)
                                                        GTCTTTTTCATCATGCGTTCAATGGGCGAAATGTTGTTCCTCGAACCGGTTACCGGTTCGTTCGCCGTTTATGCGCATCGTTATATGAGCCCGT                                       >  SRR4302537.5902007/1‑94 (MQ=60)
                                                         TCTTTTTCATCATGCGTTCAATGGGCGAAATGTTGTTCCTCGAACCGGTTACCGGTTCGTTCGCCGTTTATGCGCCTCGTTATATGAGCCCGTT                                      >  SRR4302537.5814458/1‑94 (MQ=60)
                                                         TCTTTTTCATCATGCGTTCAATGGGCGAAATGTTGTTCCTCGAACCGGTTACCGGTTCGTTCGCCGTTTATGCGCATCGTTATATGAGCCCGTT                                      >  SRR4302537.1408287/1‑94 (MQ=60)
                                                         TCTTTTTCATCATGCGTTCAATGGGCGAAATGTTGTTCCTCGAACCGGTTACCGGTTCGTTCGCCGTTTATGCGCATCGTTATATGAGCCCGTT                                      >  SRR4302537.708939/1‑94 (MQ=60)
                                                          CTTTTTCATCATGCGTTCAATGGGCGAAATGTTGTTCCTCGAACCGGTTACCGGTTCGTTCGCCGTTTATGCGCATCGTTATATGAGCCCGTTC                                     <  SRR4302537.4184788/94‑1 (MQ=60)
                                                           TTTTTCATCATGCGTTCAATGGGCGAAATGTTGTTCCTCGAACCGGTTACCGGTTCGTTCGCCGTTTATGCGCATCGTTATATGAGCCCGTTCT                                    >  SRR4302537.4096871/1‑94 (MQ=60)
                                                            TTTTCATCATGCGTTCAATGGGCGAAATGTTGTTCCTCGAACCGGTTACCGGTTCGTTCGCCGTTTATGCGCATCGTTATATGAGCCCGTTCTT                                   <  SRR4302537.1233404/94‑1 (MQ=60)
                                                             TTTCATCATGCGTTCAATGGGCGAAATGTTGTTCCTCGAACCGGTTACCGGTTCGTTCGCCGTTTATGCGCATCGTTATATGAGCCCGTTCTTT                                  >  SRR4302537.4014584/1‑94 (MQ=60)
                                                             TTTCATCATGCGTTCAATGGGCGAAATGTTGTTCCTCGAACCGGTTACCGGTTCGTTCGCCGTTTATGCGCATCGTTATATGAGCCCGTTCTTT                                  >  SRR4302537.5441675/1‑94 (MQ=60)
                                                              TTCATCATGCGTTCAATGGGCGAAATGTTGTTCCTCGAACCGGTTACCGGTTCGTTCGCCGTTTATGCGCATCGTTATATGAGCCCGTTCTTTG                                 <  SRR4302537.5659086/94‑1 (MQ=60)
                                                               TCATCATGCGTTCAATGGGCGAAATGTTGTTCCTCGAACCGGTTACCGGTTCGTTCGCCGTTTATGCGCATCGTTATATGAGCCCGTTCTTTGG                                <  SRR4302537.5638290/94‑1 (MQ=60)
                                                                CATCATGCGTTCAATGGGCGAAATGTTGTTCCTCGAACCGGTTACCGGTTCGTTCGCCGTTTATGCGCATCGTTATATGAGCCCGTTCTTTGGC                               >  SRR4302537.1957818/1‑94 (MQ=60)
                                                                 ATCATGCGTTCAATGGGCGAAATGTTGTTCCTCGAACCGGTTACCGGTTCGTTCGCCGTTTATGCGCATCGTTATATGAGCCCGTTCTTTGGCT                              <  SRR4302537.945105/94‑1 (MQ=60)
                                                                 ATCATGCGTTCAATGGGCGAAATGTTGTTCCTCGAACCGGTTACCGGTTCGTTCGCCGTTTATGCGCATCGTTATATGAGCCCGTTCTTTGGCT                              >  SRR4302537.3576252/1‑94 (MQ=60)
                                                                 ATCATGCGTTCAATGGGCGAAATGTTGTTCCTCGAACCGGTTACCGGTTCGTTCGCCGTTTATGCGCATCGTTATATGAGCCCGTTCTTTGGCT                              <  SRR4302537.4668027/94‑1 (MQ=60)
                                                                   TATGCGTTCAATGGGCGAAATGTTGTTCCTCGAACCGGTTACCGGTTCGTTCGCCGTTTATGCGCATCGTTATATGAGCCCGTTCTTTGGCTAT                            <  SRR4302537.2184783/94‑1 (MQ=60)
                                                                   CATGCGTTCAATGGGCGAAATGTTGTTCCTCGAACCGGTTACCGGTTCGTTCGCCGTTTATGCGCATCGTTATATGAGCCCGTTCTTTGGCTAT                            <  SRR4302537.2268644/94‑1 (MQ=60)
                                                                    ATGCGTTCAATGGGCGAAATGTTGTTCCTCGAACCGGTTACCGGTTCGTTCGCCGTTTATGCGCATCGTTATATGAGCCCGTTCTTTGGCTATC                           >  SRR4302537.5100113/1‑94 (MQ=60)
                                                                    ATGCGTTCAATGGGCGAAATGTTGTTCCTCGAACCGGTTACCGGTTCGTTCGCCGTTTATGCGCATCGTTATATGAGCCCGTTCTTTGGCTATC                           <  SRR4302537.5811811/94‑1 (MQ=60)
                                                                     TGCGTTCAATGGGCGAAATGTTGTTCCTCGAACCGGTTACCGGTTCGTTCGCCGTTTATGCGCATCGTTATATGAGCCCGTTCTTTGGCTATCT                          <  SRR4302537.1714725/94‑1 (MQ=60)
                                                                      GCGTTCAATGGGCGAAATGTTGTTCCTCGAACCGGTTACCGGTTCGTTCGCCGTTTATGCGCATCGTTATATGAGCCCGTTCTTTGGCTATCTC                         >  SRR4302537.3582976/1‑94 (MQ=60)
                                                                      GCGTTCAATGGGCGAAATGTTGTTCCTCGAACCGGTTACCGGTTCGTTCGCCGTTTATGCGCATCGTTATATGAGCCCGTTCTTTGGCTATCTC                         <  SRR4302537.4753097/94‑1 (MQ=60)
                                                                       CGTTCAGTGGGCGAAAGGTTGTTCCTCGAACCGGTTACCGGTTCGTTCGCCGTTTATGCGCATCGTTATATGAGCCCGTTCTTTGGCTATCTCA                        <  SRR4302537.475416/94‑1 (MQ=60)
                                                                       CGTTCAATGGGCGAAATGTTGTTCCTCGAACCGGTTACCGGTTCGTTCGCCGTTTATGCGCATCGTTATATGAGCCCGTTCTTTGGCTATCTCA                        <  SRR4302537.866474/94‑1 (MQ=60)
                                                                       CGTTCAATGGGCGAAATGTTGTTCCTCGAACCGGTTACCGGTTCGTTCGCCGTTTATGCGCATCGTTATATGAGCCCGTTCTTTGGCTATCTCA                        >  SRR4302537.162627/1‑94 (MQ=60)
                                                                        GTTCAATGGGCGAGATGTTGTTCCTCGAACCGGTTACCGGTTCGTTCGCCGTTTATGCGCATCGTTATATGAGCCCGTTCTTTGGCTATCTCAC                       >  SRR4302537.3510862/1‑94 (MQ=60)
                                                                         TTCAATGGGCGAAATGTTGTTCCTCGAACCGGTTACCGGTTCGTTCGCCGTTTATGCGCATCGTTATATGAGCCCGGTCTTTGGCTATCTCACC                      >  SRR4302537.2513202/1‑94 (MQ=60)
                                                                          TCAACGGGCGAAATGTTGTTCCTCGAACCGGTTACCGGTTCGTTCGCCGTTTATGCGCATCGTTATATGAGCCCGTTCTTTGGCTATCTCACCG                     <  SRR4302537.1345006/94‑1 (MQ=60)
                                                                           CAATGGGCGAAATGTTGTTCCTCGAACCGGTTACCGGTTCGTTCGCCGTTTATGCGCATCGTTATATGAGCCCGTTCTTTGGCTATCTCACCGC                    >  SRR4302537.2026811/1‑94 (MQ=60)
                                                                           CAATGGGCGAAATGTTGTTCCTCGAACCGGTTACCGGTTCGTTCGCCGTTTATGCGCATCGTTATATGAGCCCGTTCTTTGGCTATCTCACCGC                    >  SRR4302537.550904/1‑94 (MQ=60)
                                                                           CAATGGGCGAAATGTTGTTCCTCGAACCGGTTACCGGTTCGATCGCCGTTTATGCGCATCGTTATATGAGCCCGTTCTTTGGCTATCTCACCGC                    >  SRR4302537.2026809/1‑94 (MQ=60)
                                                                            AATGGGCGAAATGTTGTTCCTCGAACCGGTTACCGGTTCCTTCCACGTTTATGCGCATCGTTATATGAGCCCGTTCTTTGGCTATCTCACCCCC                   >  SRR4302537.3076522/1‑94 (MQ=60)
                                                                              TGGGCGAAATGTTGTTCCTCGAACCGGTTACCGGTTCGTTCGCCGTTTATGCGCATCGTTATATGAGCCCGTTCTTTGGCTATCTCACCGCCTG                 <  SRR4302537.6252/94‑1 (MQ=60)
                                                                              TGGGCGAAATGTTGTTCCTCGAACCGGTTACCGGTTCGTTCGCCGTTTATGCGCATCGTTATATGAGCCCGTTCTTTGGCTATCTCACCGCCTG                 <  SRR4302537.4143052/94‑1 (MQ=60)
                                                                               GGGCGAAATGTTGTTCCTCGAACCGGTTACCGGTTCGTTTGCCGTTTATGCGCATCGTTATATGAGCCCGTTCTTTGGCTATCTCACCGCCTGG                <  SRR4302537.806921/94‑1 (MQ=60)
                                                                               GGGCGAAATGTTGTTCCTCGAACCGGTTACCGGTTCGTTCGCCGTTTATGCGCATCGTTATATGAGCCCGTTCTTTGGCTATCTCACCGCCTGG                <  SRR4302537.3461415/94‑1 (MQ=60)
                                                                               GGGCGAAATGTTGTTCCTCGAACCGGTTACCGGTTCGTTCGCCGTTTATGCGCATCGTTATATGAGCCCGTTCTTTGGCTATCTCACCGCCTGG                >  SRR4302537.2255142/1‑94 (MQ=60)
                                                                                  CGAAATGTTGTTCCTCGAACCGGTTACCGGTTCGTTCGCCGTTTATGCGCATCGTTATATGAGCCCGTTCTTTGGCTATCTCACCGCCTGGTCT             <  SRR4302537.4938066/94‑1 (MQ=60)
                                                                                  CGAAATGTTGTTCCTCGAACCGGTTACCGGTTCGTTCGCCGTTTATGCGCATCGTTATATGAGCCCGTTCTTTGGCTATCTCACCGCCTGGTCT             <  SRR4302537.3458474/94‑1 (MQ=60)
                                                                                   GAAATGTTGTTCCTCGAACCGGTTACCGGTTCGTTCGCCGTTTATGCGCATCGTTATATGAGCCCGTTCTTTGGCTATCTCACCGCCTGGTCTT            <  SRR4302537.1820451/94‑1 (MQ=60)
                                                                                   GAAATGTTGTTCCTCGAACCGGTTACCGGTTCGTTCGCCGTTTATGCGCATCGTTATATGAGCCCGTTCTTTGGCTATCTCACCGCCTGGTCTT            >  SRR4302537.6234459/1‑94 (MQ=60)
                                                                                     AATGTTGTTCCTCGAACCGGTTACCGGTTCGTTCGCCGTTTATGCGCATCGTTATATGAGCCCGTTCTTTGGCTATCTCACCGCCTGGTCTTAC          >  SRR4302537.4437135/1‑94 (MQ=60)
                                                                                      ATGTTGTTCCTCGAACCGGTTACCGGTTCGTTCGCCGTTTATGCGCATCGTTATATGAGCCCGTTCTTTGGCTATCTCACCGCCTGGTCTTACT         >  SRR4302537.1092321/1‑94 (MQ=60)
                                                                                      ATGTTGTTCCTCGAACCGGTTACCGGTTCGTTCGCCGTTTATGCGCATCGTTATATGAGCCCGTTCTTTGGCTATCTCACCGCCTGGTCTTACT         <  SRR4302537.2786759/94‑1 (MQ=60)
                                                                                       TGTTGTTCCTCGAACCGGTTACCGGTTCGTTCGCCGTTTATGCGCATCGTTATATGAGCCCGTTCTTTGGCTATCTCACCGCCTGGTCTTACTG        <  SRR4302537.3022688/94‑1 (MQ=60)
                                                                                       TGTTGTTCCTCGAACCGGTTACCGGTTCGTTCGCCGTTTATGCGCATCGTTATATGAGCCCGTTCTTTGGCTATCTCACCGCCTGGTCTTACTG        >  SRR4302537.6456436/1‑94 (MQ=60)
                                                                                       TGTTGTTCCTCGAACCGGTTACCGGTTCGTTCGCCGTTTATGCGCATCGTTATATGAGCCCGTTCTTTGGCTATCTCACCGCCTGGTCTTACTG        <  SRR4302537.1785286/94‑1 (MQ=60)
                                                                                       TGTTGTTCCTCGAACCGGTTACCGGTTCGTTCGCCGTTTATGCGCATCGTTATATGAGCCCGTTCTTTGGCTATCTCACCGCCTGGTCTTACTG        >  SRR4302537.5391608/1‑94 (MQ=60)
                                                                                       TGTTGTTCCTCGAACCGGTTACCGGTTCGTTCGCCGTTTATGCGCATCGTTATATGAGCCCGTTCTTTGGCTATCTCACCGCCTGGTCTTACTG        <  SRR4302537.2938989/94‑1 (MQ=60)
                                                                                         TTGTTCCTCGAACCGGTTACCGGTTCGTTCGCCGTTTATGCGCATCGTTATATGAGCCCGTTCTTTGGCTATCTCACCGCCTGGTCTTACTGGT      <  SRR4302537.5757752/94‑1 (MQ=60)
                                                                                           GTTCCTCGAACCGGTTACCGGTTCGTTCGCCGTTTATGCGCATCGTTATATGAGCCCGTTCTTTGGCTATCTCACCGCCTGGTCTTACTGGTTT    <  SRR4302537.4401961/94‑1 (MQ=60)
                                                                                            TTCCTCGAACCGGTTACCGGTTCGTTCGCCGTTTATGCGCATCGTTATATGAGCCCGTTCTTTGGCTATCTCACCGCCTGGTCTTACTGGTTTA   <  SRR4302537.3205832/94‑1 (MQ=60)
                                                                                             TCCTCGAACCGGTTACCGGTTCGTTCGCCGTTTATGCGCATCGTTATATGAGCCCGTTCTTTGGCTATCTCACCGCCTGGTCTTACTGGTTTAT  >  SRR4302537.2742130/1‑94 (MQ=60)
                                                                                             |                                                                                             
CCCTGAAATGGGCCGGGCCATCCGTATTGTTGGCCTATATCATCGCCGGGCTGTTCGTCTTTTTCATCATGCGTTCAATGGGCGAAATGTTGTCCCTCGAACCGGTTACCGGTTCGTTCGCCGTTTATGCGCATCGTTATATGAGCCCGTTCTTTGGCTATCTCACCGCCTGGTCTTACTGGTTTAT  >  NC_000913/3978609‑3978795

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 7 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑