Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I2 R1 882 254.4 12957588 98.0% 12698436 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,987,586 2 bp→GC coding (814‑815/963 nt) hemC ← porphobilinogen deaminase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,987,5860CG100.0% 668.7 / NA 196R272A (CGG→GCG) hemCporphobilinogen deaminase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base G (87/109);  total (87/109)
*NC_0009133,987,5870GC100.0% 668.1 / NA 196R272A (CGG→GCG) hemCporphobilinogen deaminase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base C (87/109);  total (87/109)

Only 100 of 204 total aligned reads displayed.

GCTCTTCTGCCAGCGAAATCCCCATTTGTTCGGCATCTTGCGGCGCACCGCGGCGTTCACCGCGAATAATCTGCGAACCGTCCGGCCGGCCGACCAGCGCACGCAGCCAGATTTCGCCATCAATAAGCTCGGCGTAGCTACCAATTGGCACCTGACATCCGCCTTCGAGACGGG  >  NC_000913/3987500‑3987673
                                                                                      ||                                                                                      
gCTCTTCTGCCAGCGAAATCCCCATTTGTTCGGCATCTTGCGGCGCGCCGCGGCGTTCACCGCGAATAATCTGCGAACCGTCCGGCGCGCCGa                                                                                   >  2:4563873/1‑93 (MQ=255)
    ttctGCCAGCGAAATCCCCATTTGTTCGGCATCTTGCGGCGCGCCGCGGCGTTCACCGCGAATAATCTGCGAACCGTCCGGCGCGCCGACCAg                                                                               <  1:3613924/93‑1 (MQ=255)
     tctGCCAGCGAAATCCCCATTTGTTCGGCATCTTGCGGCGCGCCGCGGCGTTCACCGCGAATAATCTGCGAACCGTCCGGCGCGCCGACCAgc                                                                              <  1:274289/93‑1 (MQ=255)
     tctGCCAGCGAAATCCCCATTTGTTCGGCATCTTGCGGCGCGCCGCGGCGTTCACCGCGAATAATCTGCGAACCGTCCGGCGCGCCGACCAgc                                                                              <  1:3701742/93‑1 (MQ=255)
       tGCCAGCGAAATCCCCATTTGTTCGGCATCTTGCGGCGCGCCGCGGCGTTCACCGCGAATAATCTGCGAACCGTCCGGCGCGCCGACCAgcgc                                                                            >  1:3219950/1‑93 (MQ=255)
       tGCCAGCGAAATCCCCATTTGTTCGGCATCTTGCGGCGCGCCGCGGCGTTCACCGCGAATAATCTGCGAACCGTCCGGCGCGCCGACCAgcgc                                                                            >  1:2114813/1‑93 (MQ=255)
       tGCCAGCGAAATCCCCATTTGTTCGGCATCTTGCGGCGCGCCGCGGCGTTCACCGCGAATAATCTGCGAACCGTCCGGCGCGCCGACCAgcgc                                                                            >  2:3617264/1‑93 (MQ=255)
        gCCAGCGAAATCCCCATTTGTTCGGCATCTTGCGGCGCGCCGCGGCGTTCACCGCGAATAATCTGCGAACCGTCCGGCGCGCCGACCAGcgca                                                                           <  2:3238159/93‑1 (MQ=255)
         ccAGCGAAATCCCCATTTGTTCGGCATCTTGCGGCGCGCCGCGGCGTTCACCGCGAATAATCTGCGAACCGTCCGGCGCTCCGACCAGcgcac                                                                          <  2:280352/93‑1 (MQ=255)
         ccAGCGAAATCCCCATTTGTTCGGCATCTTGCGGCGCGCCGCGGCGTTCACCGCGAATAATCTGCGAACCGTCCGGCGCGCCGACCAGcgcac                                                                          >  2:3959440/1‑93 (MQ=255)
          cAGCGAAATCCCCATTTGTTCGGCATCTTGCGGCGCGCCGCGGCGTTCACCGCGAATAATCTGCGAACCGTCCGGCGCGCCGACCAGcgcacg                                                                         <  1:5218210/93‑1 (MQ=255)
          cAGCGAAATCCCCATTTGTTCGGCATCTTGCGGCGCGCCGCGGCGTTCACCGCGAATAATCTGCGAACCGTCCGGCGCGCCGACCAGcgcacg                                                                         >  2:3152791/1‑93 (MQ=255)
            gCGAAATCCCCATTTGTTCGGCATCTTGCGGCGCGCCGCGGCGTTCACCGCGAATAATCTGCGAACCGTCCGGCGCGCCGACCAGcgcacgca                                                                       >  2:3814162/1‑93 (MQ=255)
            gCGAAATCCCCATTTGTTCGGCATCTTGCGGCGCGCCGCGGCGTTCACCGCGAATAATCTGCGAACCGTCCGGCGCGCCGACCAGcgcacgca                                                                       >  2:5310762/1‑93 (MQ=255)
              gAAATCCCCATTTGTTCGGCATCTTGCGGCGCGCCGCGGCGTTCACCGCGAATAATCTGCGAACCGTCCGGCGCGCCGACCAGCGCACGCAGc                                                                     >  2:715762/1‑93 (MQ=255)
                aaTCCCCATTTGTTCGGCATCTTGCGGCGCGCCGCGGCGTTCACCGCGAATAATCTGCGAACCGTCCGGCGCGCCGACCAGCGCACGCAGCCa                                                                   <  1:4241941/93‑1 (MQ=255)
                aaTCCCCATTTGTTCGGCATCTTGCGGCGCGCCGCGGCGTTCACCGCGAATAATCTGCGAACCGTCCGGCGCGCCGACCAGCGCACGCAGCCa                                                                   >  1:6178889/1‑93 (MQ=255)
                aaTCCCCATTTGTTCGGCATCTTGCGGCGCGCCGCGGCGTTCACCGCGAATAATCTGCGAACCGTCCGGCGCGCCGACCAGCGCACGCAGCCa                                                                   <  1:1132423/93‑1 (MQ=255)
                 aTCCCCATTTGTTCGGCATCTTGCGGCGCGCCGCGGCGTTCACCGCGAATAATCTGCGAACCGTCCGGCGCGCCGACCAGCGCACGCAGCCAg                                                                  >  1:3406178/1‑93 (MQ=255)
                 aTCCCCATTTGTTCGGCATCTTGCGGCGCGCCGCGGCGTTCACCGCGAATAATCTGCGAACCGTCCGGCGCGCCGACCAGCGCACGCAGCCAg                                                                  >  1:6245962/1‑93 (MQ=255)
                  tCCCCATTTGTTCGGCATCTTGCGGCGCGCCGCGGCGTTCACCGCGAATAATCTGCGAACCGTCCGGCGCGCCGACCAGCGCACGCAGCCAGa                                                                 >  1:1424160/1‑93 (MQ=255)
                    cccATTTGTTCGGCATCTTGCGGCGCGCCGCGGCGTTCACCGCGAATAATCTGCGAACCGTCCGGCGCGCCGACCAGCGCACGCAGCCAGAtt                                                               >  1:3491628/1‑93 (MQ=255)
                    cccATTTGTTCGGCATCTTGCGGCGCGCCGCGGCGTTCACCGCGAATAATCTGCGAACCGTCCGGCGCGCCGACCAGCGCACGCAGCCAGAtt                                                               >  2:1459140/1‑93 (MQ=255)
                     ccATTTGTTCGGCATCTTGCGGCGCGCCGCGGCGTTCACCGCGAATAATCTGCGAACCGTCCGGCGCGCCGACCAGCGCACGCAGCCAGAttt                                                              <  1:3899738/93‑1 (MQ=255)
                      cATTGGTTCGGCATCTTGCGGCGCGCCGCGGCGTTCACCGCGAATAATCTGCGAACCGTCCGGCGCGCCGACCAGCGCACGCAGCCAGATTTc                                                             <  1:3843570/93‑1 (MQ=255)
                        tttGTTCGGCATCTTGCGGCGCGCCGCGGCGTTCACCGCGAATAATCTGCGAACCGTCCGGCGCGCCGACCAGCGCACGCAGCCAGATTTCGc                                                           >  2:2478609/1‑93 (MQ=255)
                        tttGTTCGGCATCTTGCGGCGCGCCGCGGCGTTCACCACGAATAATCTGCGAACCGTCCGGCGCGCCGACCAGCGCACGCAGCCAGATTTCGc                                                           <  2:6191162/93‑1 (MQ=255)
                          tGTTCGGCATCTTGCGGCGCGCCGCGGCGTTCACCGCGAATAATCTGCGAACCGTCCGGCGCGCCGACCAGCGCACGCAGCCAGATTTCGCCa                                                         >  1:3829682/1‑93 (MQ=255)
                          tGTTCGGCATCTTGCGGCGCGCCGCGGCGTTCACCGCGAATAATCTGCGAACCGTCCGGCGCGCCGACCAGCGCACGCAGCCAGATTTCGCCa                                                         <  1:5101385/93‑1 (MQ=255)
                           gTTCGGCATCTTGCGGCGCGCCGCGGCGTTCACCGCGAATAATCTGCGAACCGTCCGGCGCGCCGACCAGCGCACGCAGCCAGATTTCGCCAt                                                        >  2:1188331/1‑93 (MQ=255)
                             tCGGCATCTTGCGGCGCGCCGCGGCGTTCACCGCGAATAATCTGCGAACCGTCCGGCGCGCCGACCAGCGCACGCAGCCAGATTTCGCCATCa                                                      <  1:2926218/93‑1 (MQ=255)
                             tCGGCATCTTGCGGCGCGCCGCGGCGTTCACCGCGAATAATCTGCGAACCGTCCGGCGCGCCGACCAGCGCACGCAGCCAGATTTCGCCATCa                                                      <  1:6148746/93‑1 (MQ=255)
                              cGGCATCTGGCGGCGCGCCGCGGCGTTCACCGCGAATAATCTGCGAACCGTCCGGCGCGCCGACCAGCGCACGCAGCCAGATTTCGCCATCaa                                                     <  1:4608476/93‑1 (MQ=255)
                               ggCATCTTGCGGCGCGCCGCGGCGTTCACCGCGAATAATCTGCGAACCGTCCGGCGCGCCGCCCAGCGCACGCAGCCAGATTTCGCCATCAAt                                                    <  2:4731392/93‑1 (MQ=255)
                               ggCATCTTGCGGCGCGCCGCGGCGTTCACCGCGAATAATCTGCGAACCGTCCGGCGCGCCGACCAGCGCACGCAGCCAGATTTCGCCATCAAt                                                    >  2:1996545/1‑93 (MQ=255)
                                gCATCTTGCGGCGCGCCGCGGCGTTCACCGCGAATAATCTGCGAACCGTCCGGCGCGCCGACCAGCGCACGCAGCCAGATTTCGCCATCAATa                                                   <  2:826647/93‑1 (MQ=255)
                                gCATCTTGCGGCGCGCCGCGGCGTTCACCGCGAATAATCTGCGAACCGTCCGGCGCGCCGACCAGCGCACGCAGCCAGATTTCGCCATCAATa                                                   <  1:6054290/93‑1 (MQ=255)
                                gCATCTTGCGGCGCGCCGCGGCGTTCACCGCGAATAATCTGCGAACCGTCCGGCGCGCCGACCAGCGCACGCAGCCAGATTTCGCCATCAATa                                                   <  1:1471796/93‑1 (MQ=255)
                                gCATCTTGCGGCGCGCCGCGGCGTTCACCGCGAATAATCTGCGAACCGTCCGGCGCGCCGACCAGCGCACGCAGCCAGATTTCGCCATCAATa                                                   <  1:165480/93‑1 (MQ=255)
                                gCATCTTGCGGCGCGCCGCGGCGTTCACCGCGAATAATCTGCGAACCGTCCGGCGCGCCGACCAGCGCACGCAGCCAGATTTCGCCATCAATa                                                   <  1:3971243/93‑1 (MQ=255)
                                 cATCTTGCGGCGCGCCGCGGCGTTCACCGCGAATAATCTGCGAACCGTCCGGCGCGCCGACCAGCGCACGCAGCCAGATTTCGCCATCAATaa                                                  >  2:4870495/1‑93 (MQ=255)
                                 cATCTTGCGGCGCGCCGCGGCGTTCACCGCGAATAATCTGCGAACCGTCCGGCGCGCCGACCAGCGCACGCAGCCAGATTTCGCCATCAATaa                                                  <  2:3382615/93‑1 (MQ=255)
                                 cATCTTGCGCGGGGCAGCGGCGTTCAGGGCGAATAATCTGCGACCCGTCCGGCGCTACGACCCGCGAACGCAGCCAGATTTCGCCataaataa                                                  <  2:5715976/93‑1 (MQ=255)
                                  aTCTTGCGGCGCGCCGCGGCGTTCACCGCGAATAATCTGCGAACCGTCCGGCGCGCCGACCAGCGCACGCAGCCAGATTTCGCCATCAATAAg                                                 >  1:5317575/1‑93 (MQ=255)
                                   tCTTGCGGCGCGCCGCGGCGTTCACCGCGAATAATCTGCGAACCGTCCGGCGCGCCGACCAGCGCACGCAGCCAGATTTCGCCATCAATAAGc                                                <  2:449953/93‑1 (MQ=255)
                                   tCTTGCGGCGCGCCGCGGCGTTCACCGCGAATAATCTGCGAACCGTCCGGCGCGCCGACCAGCGCACGCAGCCAGATTTCGCCATCAATAAGc                                                >  2:2266140/1‑93 (MQ=255)
                                     ttGCGGCGCGCCGCGGCGTTCACCGCGAATAATCTGCGAACCGTCCGGCGCGCCGACCAGCGCACGCAGCCAGATTTCGCCATCAATAAGCTc                                              <  1:4798565/93‑1 (MQ=255)
                                      tGCGGCGCGCCGCGGCGTTCACCGCGAATAATCTGCGAACCGTCCGGCGCGCCGACCAGCGCACGCAGCCAGATTTCGCCATCAATAAGCTCg                                             <  2:653004/93‑1 (MQ=255)
                                        cggcgCGCCGCGGCGTTCACCGCGAATAATCTGCGAACCGTCCGGCGCGCCGACCAGCGCACGCAGCCAGATTTCGCCATCAATAAGCTCGGc                                           <  1:1404417/93‑1 (MQ=255)
                                            gcgccGCGGCGTTCACCGCGAATAATCTGCGAACCGTCCGGCGCGCCGACCAGCGCACGCAGCCAGATTTCTCCATCAATAAGCTCGGCGTAg                                       >  2:4459859/4‑93 (MQ=255)
                                            gcgccGCGGCGTTCACCGCGAATAATCTGCGAACCGTCCGGCGCGCCGACCAGCGCACGCAGCCAGATTTCGCCATCAATAAGCTCGGCGTAg                                       >  2:4550565/4‑93 (MQ=255)
                                              gccGCGGCGTTCACCGCGAATAATCTGCGAACCGTCCGGCGCGCCGACCAGCGCACGCAGCCAGATTTCGCCATCAATAAGCTCGGCGTAGCt                                     >  2:2957039/2‑93 (MQ=255)
                                              gccGCGGCGTTCACCGCGAATAATCTGCGAACCGTCCGGCGCGCCGACCAGCGCACGCAGCCAGATTTCGCCATCAATAAGCTCGGCGTAGCt                                     <  2:266866/92‑1 (MQ=255)
                                              gccGCGGCGTTCACCGCGAATAATCTGCGAACCGTCCGGCGCGCCGACCAGCGCACGCAGCCAGATTTCGCCATCAATAAGCTCGGCGTAGCt                                     <  2:6432268/92‑1 (MQ=255)
                                              gccGCGGCGTTCACCGCGAATAATCTGCGAACCGTCCGGCGCGCCGACCAGCGCACGCAGCCAGATTTCGCCATCAATAAGCTCGGCGTAGCt                                     >  2:1369009/2‑93 (MQ=255)
                                                 gcggcgTTCACCGCGAATAATCTGCGAACCGTCCGGCGCGCCGACCAGCGCACGCAGCCAGATTTCGCCATCAATAAGCTCGGCGTAGCTAcc                                  >  1:3202422/1‑93 (MQ=255)
                                                 gcggcgTTCACCGCGAATAATCTGCGAACCGTCCGGCGCGCCGACCAGCGCACGCAGCCAGATTTCGCCATCAATAAGCTCGGCGTAGCTAcc                                  >  2:1804486/1‑93 (MQ=255)
                                                 gcggcgTTCACCGCGAATAATCTGCGAACCGTCCGGCGCGCCGACCAGCGCACGCAGCCAGATTTCGCCATCAATAAGCTCGGCGTAGCTAcc                                  >  2:1119936/1‑93 (MQ=255)
                                                 gcggcgTTCACCGCGAATAATCTGCGAACCGTCCGGCGCGCCGACCAGCGCACGCAGCCAGATTTCGCCATCAATAAGCTCGGCGTAGCTAcc                                  >  2:3738368/1‑93 (MQ=255)
                                                   ggcgTTCACCGCGAATAATCTGCGAACCGTCCGGCGCGCCGACCAGCGCACGCAGCCAGATTTCGCCATCAATAAGCTCGGCGTAGCTACCaa                                <  2:3860611/93‑1 (MQ=255)
                                                   ggcgTTCACCGCGAATAATCTGCGAACCGTCCGGCGCGCCGACCAGCGCACGCAGCCAGATTTCGCCATCAATAAGCTCGGCGGAGCTACCaa                                >  2:5962805/1‑93 (MQ=255)
                                                    gcgTTCACGGCGAATAATCTGCGAACCGTCCGGCGCGCCGACCAGCGCACGCAGCCAGATTTCGCCATCAATAAGCTCGGCGTAGCTTCCAAt                               <  2:4086731/93‑1 (MQ=255)
                                                    gcgTTCACCGCGAATAATCTGCGAACCGTCCGGCGCGCCGACCAGCGCACGCAGCCAGATTTCGCCATCAATAAGCTCGGCGTAGCTACCAAt                               <  2:4241281/93‑1 (MQ=255)
                                                    gcgTTCACCGCGAATAATCTGCGAACCGTCCGGCGCGCCGACCAGCGCACGCAGCCAGATTTCGCCATCAATAAGCTCGGCGTAGCTACCAAt                               <  1:4500804/93‑1 (MQ=255)
                                                    gcgTTCACCGCGAATAATCTGCGAACCGTCCGGCGCGCCGACCAGCGCACGCAGCCAGATTTCGCCATCAATAAGCTCGGCGTAGCTACCAAt                               <  1:2361492/93‑1 (MQ=255)
                                                    gcgTTCACCGCGAATAATCTGCGAACCGTCCGGCGCGCCGACCAGCGCACGCAGCCAGATTTCGCCATCAATAAGCTCGGCGTAGCTACCAAt                               <  2:6089711/93‑1 (MQ=255)
                                                      gTTCACCGCGAATAATCTGCGAACCGTCCGGCGCGCCGACCAGCGCACGCAGCCAGATTTCGCCATCAATAAGCTCGGCGTAGCTACCAATTg                             >  1:5568551/1‑93 (MQ=255)
                                                      gTTCACCGCGAATAATCTGCGAACCGTCCGGCGCGCCGACCAGCGCACGCAGCCAGATTTCGCCATCAATAAGCTCGGCGTAGCTACCAATTg                             <  1:1310442/93‑1 (MQ=255)
                                                      gTTCACCGCGAATAATCTGCGAACCGTCCGGCGCGCCGACCAGCGCACGCAGCCAGATTTCGCCATCAATAAGCTCGGCGTAGCTACCAATTg                             <  2:3640/93‑1 (MQ=255)
                                                       ttCACCGCGAATAATCTGCGAACCGTCCGGCGCGCCGACCAGCGCACGCAGCCAGATTTCGCCATCAATAAGCTCGGCGTAGCTACCAATTgg                            >  1:4228924/1‑93 (MQ=255)
                                                       ttCACCGCGAATAATCTGCGAACCGTCCGGCGCGCCGACCAGCGCACGCAGCCAGATTTCGCCATCAATAAGCTCGGCGTAGCTACCAATTgg                            <  2:3321737/93‑1 (MQ=255)
                                                        tCACCGCGCATAATCTGCGAACCGTCCGGCGCGCCGACCAGCGCACGCAGCCAGATTTCGCCATCAATAAGCTCGGCGTAGCTACCAATTGGc                           >  2:6318534/1‑93 (MQ=255)
                                                        tCACCGCGAATAATCTGCGAACCGTCCGGCGCGCCGACCAGCGCACGCAGCCAGATTTCGCCATCAATAAGCTCGGCGTAGCTACCAATTGGc                           >  1:2976173/1‑93 (MQ=255)
                                                         cACCGCGAATAATCTGCGAACCGTCCGGCGCGACGACCAGCGCACGCAGCCAGATTTCGCCATCAATAAGCTCGGCGTAGCTACCAATTGGCa                          <  1:3156313/93‑1 (MQ=255)
                                                            cgcgAATAATCTGCGAACCGTCCGGCGCGCCGACCAGCGCACGCAGCCAGATTTCGCCATCAATAAGCTCGGCGTAGCTACCAATTGGCACCt                       <  1:3721770/93‑1 (MQ=255)
                                                             gcgAATAATCTGCGAACCGTCCGGCGCGCCGACCAGCGCACGCAGCCAGATTTCGCCATCAATAAGCTCGGCGTAGCTACCAATTGGCACCTg                      >  2:5833341/1‑93 (MQ=255)
                                                              cgAATAATCTGCGAACCGTCCGGCGCGCCGACCAGCGCACGCAGCCAGATTTCGCCATCAATAAGCTCGGCGTAGCTACCAATTGGCACCTGa                     <  2:3361593/93‑1 (MQ=255)
                                                              cgAATAATCTGCGAACCGTCCGGCGCGCCGACCAGCGCACGCAGCCAGATTTCGCCATCAATAAGCTCGGCGTAGCTACCAATTGGCACCTGa                     >  1:1866978/1‑93 (MQ=255)
                                                               gAATAATCTGCGAACCGTCCGGCGCGCCGACCAGCGCACGCAGCCAGATTTCGCCATCAATAAGCTCGGCGTAGCTACCAATTGGCACCTGAc                    >  2:2066879/1‑93 (MQ=255)
                                                               gAATAATCTGCGAACCGTCCGGCGCGCCGACCAGCGCACGCAGCCAGATTTCGCCATCAATAAGCTCGGCGTAGCTACCAATTGGCACCTGAc                    >  1:6450807/1‑93 (MQ=255)
                                                                 ataatCTGCGAACCGTCCGGCGCGCCGACCAGCGCACGCAGCCAGATTTCGCCATCAATAAGCTCGGCGTAGCTACCAATTGGCACCTGACAt                  <  2:1026437/93‑1 (MQ=255)
                                                                  taatCTGCGAACCGTCCGGCGCGCCGACCAGCGCACGCAGCCAGATTTCGCCATCAATAAGCTCGGCGTAGCTACCAATTGGCACCTGACATc                 >  2:6107233/1‑93 (MQ=255)
                                                                   aatCTGCGAACCGTCCGGCGCTCCGACCAGCGCACGCAGCCAGATTTCGCCATCAATAAGCTCGGCGTAGCTACCAATTGGCACCTGACATcc                <  2:2213374/93‑1 (MQ=255)
                                                                   aatCTGCGAACCGTCCGGCGCGCCGACCAGCGCACGCAGCCAGATTTCGCCATCAATAAGCTCGGCGTAGCTACCAATTGGCACCTGACATcc                >  1:3779973/1‑93 (MQ=255)
                                                                     tCTGCGAACCGTCCGGCGCGCCGACCAGCGCACGCAGCCAGATTTCGCCATCAATAAGCTCGGCGTAGCTACCAATTGGCACCTGACATCCGc              >  1:5668645/1‑93 (MQ=255)
                                                                       tGCGAACCGTCCGGCGCGCCGACCAGCGCACGCAGCCAGATTTCGCCATCAATAAGCTCGGCGTAGCTACCAATTGGCACCTGACATCCGCCt            >  2:2133164/1‑93 (MQ=255)
                                                                       tGCGAACCGTCCGGCGCGCCGACCAGCGCACGCAGCCAGATTTCGCCATCAATAAGCTCGGCGTAGCTACCAATTGGCACCTGACATCCGCCt            >  1:6412506/1‑93 (MQ=255)
                                                                        gCGAACCGTCCGGCGCGCCGACCAGCGCACGCAGCCAGATTTCGCCATCAATAAGCTCGGCGTAGCTACCAATTGGCACCTGACATCCGCCtt           <  2:4510938/93‑1 (MQ=255)
                                                                        gCGAACCGTCCGGCGCGCCGACCAGCGCACGCAGCCAGATTTCGCCATCAATAAGCTCGGCGTAGCTACCAATTGGCACCTGACATCCGCCtt           <  2:218366/93‑1 (MQ=255)
                                                                         cGAACCGTCCTGCGCGCCGACCAGCGCACGCAGCCAGATTTCGCCATCAATAAGCTCGGCGTAGCTACCAATTGGCACCTGACATCCGCCTTc          >  2:4974220/1‑93 (MQ=255)
                                                                         cGAACCGTCCGGCGCGCCGACCAGCGCACGCAGCCAGATTTCGCCATCAATAAGCTCGGCGTAGCTACCAATTGGCACCTGACATCCGCCTTc          >  1:1557756/1‑93 (MQ=255)
                                                                          gAACCGTCCGGCGCGCCGACCAGCGCACGCAGCCAGATTTCGCCATCAATAAGCTCGGCGTAGCTACCAATTGGCACCTGACATCCGCCTTCg         <  2:5892596/93‑1 (MQ=255)
                                                                           aaCCGTCCGGCGCGCCGACCAGCGCACGCAGCCAGATTTCGCCATCAATAAGCTCGGCGTAGCTACCAATTGGCACCTGACATCCGCCTTCga        <  1:1916832/93‑1 (MQ=255)
                                                                           aaCCGTCCGGCGCGCCGACCAGCGCACGCAGCCAGATTTCGCCATCAATAAGCTCGGCGTAGCTACCAATTGGCACCTGACATCCGCCTTCga        <  2:3663374/93‑1 (MQ=255)
                                                                                tCCGGCGCGCCGACCAGCGCACGCAGCCAGATTTCGCCATCAATAAGCTCGGCGTAGCTACCAATTGGCACCTGACATCCGCCTTCGAGACgg   <  2:4785312/93‑1 (MQ=255)
                                                                                tCCGGCGCGCCGACCAGCGCACGCAGCCAGATTTCGCCATCAATAAGCTCGGCGTAGCTACCAATTGGCACCTGACATCCGCCTTCGAGACgg   <  2:2821112/93‑1 (MQ=255)
                                                                                tCCGGCGCGCCGACCAGCGCACGCAGCCAGATTTCGCCATCAATAAGCTCGGCGTAGCTACCAATTGGCACCTGACATCCGCCTTCGAGACgg   >  1:1091287/1‑93 (MQ=255)
                                                                                 ccggcgcgCCGACCAGCGCACGCAGCCAGATTTCGCCATCAATAAGCTCGGCGTAGCTACCAATTGGCACCTGACATCCGCCTTCGAGACggg  <  2:5667377/93‑1 (MQ=255)
                                                                                 ccggcgcgCCGACCAGCGCACGCAGCCAGATTTCGCCATCAATAAGCTCGGCGTAGCTACCAATTGGCACCTGACATCCGCCTTCGAGACggg  <  2:4579837/93‑1 (MQ=255)
                                                                                 ccggcgcgCCGACCAGCGCACGCAGCCAGATTTCGCCATCAATAAGCTCGGCGTAGCTACCAATTGGCACCTGACATCCGCCTTCGAGACggg  <  1:2196904/93‑1 (MQ=255)
                                                                                      ||                                                                                      
GCTCTTCTGCCAGCGAAATCCCCATTTGTTCGGCATCTTGCGGCGCACCGCGGCGTTCACCGCGAATAATCTGCGAACCGTCCGGCCGGCCGACCAGCGCACGCAGCCAGATTTCGCCATCAATAAGCTCGGCGTAGCTACCAATTGGCACCTGACATCCGCCTTCGAGACGGG  >  NC_000913/3987500‑3987673

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
TTCAGTAGCTCTTCTGCCAGCGAAATCCCCATTTGTTCGGCATCTTGCGGCGCACCGCGGCGTTCACCGCGAATAATCTGCGAACCGTCCGGCCGGCCGACCAGCGCACGCAGCCAGATTTCGCCATCAATAAGCTCGGCGTAGCTACCAATTGGCACCTGACATCCGCCTTCGAGACGGGTA  >  NC_000913/3987493‑3987675
                                                                                             |                                                                                         
TTCAGTAGCTCTTCTGCCAGCGAAATCCCCATTTGTTCGGCATCTTGCGGCGCGCCGCGGCGTTCACCGCGAATAATCTGCGAACCGTCCGGCG                                                                                           >  SRR4302537.2182703/1‑94 (MQ=60)
TTCAGTAGCTCTTCTGCCAGCGAAATCCCCATTTGTTCGGCATCTTGCGGCGCGCCGCGGCGTTCACCGCGAATAATCTGCGAACCGTCCGGCG                                                                                           <  SRR4302537.2008939/94‑1 (MQ=60)
         TCTTCTGCCAGCGAAATCCCCATTTGTTCGGCATCTTGCGGCGCGCCGCGGCGTTCACCGCGAATAATCTGCGAACCGTCCGGCGCGCCGACCA                                                                                  <  SRR4302537.635144/94‑1 (MQ=60)
           TTCTGCCAGCGAAATCCCCATTTGTTCGGCATCTTGCGGCGCGCCGCGGCGTTCACCGCGAATAATCTGCGAACCGTCCGGCGCGCCGACCAGC                                                                                <  SRR4302537.3639400/94‑1 (MQ=60)
            TCTGCCAGCGAAATCCCCATTTGTTCGGCATCTTGCGGCGCGCCGCGGCGTTCACCGCGAATAATCTGCGAACCGTCCGGCGCGCCGACCAGCG                                                                               <  SRR4302537.3727781/94‑1 (MQ=60)
            TCTGCCAGCGAAATCCCCATTTGTTCGGCATCTTGCGGCGCGCCGCGGCGTTCACCGCGAATAATCTGCGAACCGTCCGGCGCGCCGACCAGCG                                                                               <  SRR4302537.276216/94‑1 (MQ=60)
            TCTGCCAGCGAAATCCCCATGTGTCCGGCATCTTGCGGCGCACCGCGGCGTTCACCGCGAATAATCTGCGAACCGTCCGGCGCGCCGACCAGCG                                                                               <  SRR4302537.1067366/94‑1 (MQ=60)
             CTGCCAGCGAAATCCCCATTTGTTCGGCATCTTGCGGCGCGCCGCGGCGTTCACCGCGAATAATCTGCGAACCGTCCGGCGCGCCGACCAGCGC                                                                              <  SRR4302537.2173904/94‑1 (MQ=60)
             CTGCCAGCGAAATCCCCATTTGTTCGGCATCTTGCGGCGCGCCGCGGCGTTCACCGCGAATAATCTGCGAACCGTCCGGCGCGCCGACCAGCGC                                                                              >  SRR4302537.2129714/1‑94 (MQ=60)
             CTGCCAGCGAAATCCCCATTTGTTCGGCATCTTGCGGCGCGCCGCGGCGTTCACCGCGAATAATCTGCGAACCGTCCGGCGCGCCGACCAGCGC                                                                              >  SRR4302537.3242611/1‑94 (MQ=60)
             CTGCCAGCGAAATCCCCATTTGTTCGGCATCTTGCGGCGCGCCGCGGCGTTCACCGCGAATAATCTGCGAACCGTCCGGCGCGCCGACCAGCGC                                                                              >  SRR4302537.3672416/1‑94 (MQ=60)
                CCAGCGAAATCCCCATTTGTTCGGCATCTTGCGGCGCGCCGCGGCGTTCACCGCGAATAATCTGCGAACCGTCCGGCGCGCCGACCAGCGCACG                                                                           >  SRR4302537.4270810/1‑94 (MQ=60)
                CCAGCGAAATCCCCATTTGTTCGGCATCTTGCGGCGCGCCGCGGCGTTCACCGCGAATAATCTGCGAACCGTCCGGCGCGCCGACCAGCGCACG                                                                           <  SRR4302537.1108852/94‑1 (MQ=60)
                 CAGCGAAATCCCCATTTGTTCGGCATCTTGCGGCGCGCCGCGGCGTTCACCGCGAATAATCTGCGAACCGTCCGGCGCGCCGACCAGCGCACGC                                                                          <  SRR4302537.5254099/94‑1 (MQ=60)
                    CGAAATCCCCATTTGTTCGGCATCTTGCGGCGCGCCGCGGCGTTCACCGCGAATAATCTGCGAACCGTCCGGCGCGCCGACCAGCGCACGCAGC                                                                       <  SRR4302537.1428787/94‑1 (MQ=60)
                      AAATCCCCATTTGTTCGGCATCTTGCGGCGCGCCGCGGCGTTCACCGCGAATAATCTGCGAACCGTCCGGCGCGCCGACCAGCGCACGCAGCCA                                                                     >  SRR4302537.6221446/1‑94 (MQ=60)
                      AAATCCCCATTTGTTCGGCATCTTGCGGCGCGCCGCGGCGTTCACCGCGAATAATCTGCGAACCGTCCGGCGCGCCGACCAGCGCACGCAGCCA                                                                     >  SRR4302537.6221447/1‑94 (MQ=60)
                       AATCCCCATTTGTTCGGCATCTTGCGGCGCGCCGCGGCGTTCACCGCGAATAATCTGCGAACCGTCCGGCGCGCCGACCAGCGCACGCAGCCAG                                                                    <  SRR4302537.645948/94‑1 (MQ=60)
                       AATCCCCATTTGTTCGGCATCTTGCGGCGCGCCGCGGCGTTCACCGCGAATAATCTGCGAACCGTCCGGCGCGCCGACCAGCGCACGCAGCCAG                                                                    >  SRR4302537.6288884/1‑94 (MQ=60)
                       AATCCCCATTTGTTCGGCATCTTGCGGCGCGCCGCGGCGTTCACCGCGAATAATCTGCGAACCGTCCGGCGCGCCGACCAGCGCACGCAGCCAG                                                                    <  SRR4302537.4271434/94‑1 (MQ=60)
                       AATCCCCATTTGTTCGGCATCTTGCGGCGCGCCGCGGCGTTCACCGCGAATAATCTGCGAACCGTCCGGCGCGCCGACCAGCGCACGCAGCCAG                                                                    <  SRR4302537.1140285/94‑1 (MQ=60)
                       AATCCCCATTTGTTCGGCATCTTGCGGCGCGCCGCGGCGTTCACCGCGAATAATCTGCGAACCGTCCGGCGCGCCGACCAGCGCACGCAGCCAG                                                                    >  SRR4302537.3430302/1‑94 (MQ=60)
                       AATCCCCATTTGTTCGGCATCTTGCGGCGCGCCGCGGCGTTCACCGCGAATAATCTGCGAACCGTCCGGCGCGCCGACCAGCGCACGCAGCCAG                                                                    >  SRR4302537.3585884/1‑94 (MQ=60)
                        ATCCCCATTTGTTCGGCATCTTGCGGCGCGCCGCGGCGTTCACCGCGAATAATCTGCGAACCGTCCGGCGCGCCGACCAGCGCACGCAGCCAGA                                                                   >  SRR4302537.462401/1‑94 (MQ=60)
                        ATCCCCATTTGTTCGGCATCTTGCGGCGCGCCGCGGCGTTCACCGCGAATAATCTGCGAACCGTCCGGCGCGCCGACCAGCGCACGCAGCCAGA                                                                   >  SRR4302537.524563/1‑94 (MQ=60)
                        ATCCCCATTTGTTCGGCATCTTGCGGCGCGCCGCGGCGTTCACCGCGAATAATCTGCGAACCGTCCGGCGCGCCGACCAGCGCACGCAGCCAGA                                                                   >  SRR4302537.1434076/1‑94 (MQ=60)
                          CCCCATTTGTTCGGCATCTTGCGGCGCGCCGCGGCGTTCACCGCGAATAATCTGCGAACCGTCCGGCGCGCCGACCAGCGCACGCAGCCAGATT                                                                 >  SRR4302537.3516327/1‑94 (MQ=60)
                          CCCCATTTGTTCGGCATCTTGCGGCGCGCCGCGGCGTTCACCGCGAATAATCTGCGAACCGTCCGGCGCGCCGACCAGCGCACGCAGCCAGATT                                                                 <  SRR4302537.3858586/94‑1 (MQ=60)
                            CCATTTGTTCGGCATCTTGCGGCGCGCCGCGGCGTTCACCGCGAATAATCTGCGAACCGTCCGGCGCGCCGACCAGCGCACGCAGCCAGATTTC                                                               >  SRR4302537.6150055/1‑94 (MQ=60)
                            CCATTTGTTCGGCATCTTGCGGCGCGCCGCGGCGTTCACCGCGAATAATCTGCGAACCGTCCGGCGCGCCGACCAGCGCACGCAGCCAGATTTC                                                               <  SRR4302537.3927001/94‑1 (MQ=60)
                             CATTTGTTCGGCATCTTGCGGCGCTCCGCGGCGTTCACCGCGAATAATCTGCGAACCGTCCGGCGCGCCGACCAGCGCACGCAGCCAGATTTCG                                                              <  SRR4302537.1812867/94‑1 (MQ=60)
                             CATTTGTTCGGCATCTTGCGGCGCGCCGCGGCGTTCACCGCGAATAATCTGCGAACCGTCCGGCGCGCCGACCAGCGCACGCAGCCAGATTTCG                                                              >  SRR4302537.139437/1‑94 (MQ=60)
                             CATTGGTTCGGCATCTTGCGGCGCGCCGCGGCGTTCACCGCGAATAATCTGCGAACCGTCCGGCGCGCCGACCAGCGCACGCAGCCAGATTTCG                                                              <  SRR4302537.3870487/94‑1 (MQ=60)
                               TTTGTTCGGCATCTTGCGGCGCGCCGCGGCGTTCACCGCGAATAATCTGCGAACCGTCCGGCGCGCCGACCAGCGCACGCAGCCAGATTTCGCC                                                            <  SRR4302537.3449970/94‑1 (MQ=60)
                                TTGTTCGGCATCTTGCGGCGCGCCGCGGCGTTCACCGCGAATAATCTGCGAACCGTCCGGCGCGCCGACCAGCGCACGCAGCCAGATTTCGCCA                                                           >  SRR4302537.3856528/1‑94 (MQ=60)
                                TTGTTCGGCATCTTGCGGCGCACCGCGGCGTTCACCGCGAATAATCTGCGAACCGTCCGGCGCGCCGACCAGCGCACGCAGCCAGATTTCGCCA                                                           >  SRR4302537.2927620/1‑94 (MQ=60)
                                 TGTTCGGCATCTTGCGGCGCGCCGCGGCGTTCACCGCGAATAATCTGCGAACCGTCCGGCGCGCCGACCAGCGCACGCAGCCAGATTTCGCCAT                                                          <  SRR4302537.3195682/94‑1 (MQ=60)
                                 TGTTCGGCATCTTGCGGCGCGCCGCGGCGTTCACCGCGAATAATCTGCGAACCGTCCGGCGCGCCGACCAGCGCACGCAGCCAGATTTCGCCAT                                                          <  SRR4302537.5136535/94‑1 (MQ=60)
                                  GTTCGGCATCTTGCGGCGCGCCGCGGCGTTCACCGCGAATAATCTGCGAACCGTCCGGCGCGCCGACCAGCGCACGCAGCCAGATTTCGCCATC                                                         <  SRR4302537.373209/94‑1 (MQ=60)
                                    TCGGCATCTTGCGGCGCGCCGCGGCGTTCACCGCGAATAATCTGCGAACCGTCCGGCGCGCCGACCAGCGCACGCAGCCAGATTTCGCCATCAA                                                       <  SRR4302537.2946767/94‑1 (MQ=60)
                                    TCGGCATCTTGCGGCGCGCCGCGGCGTTCACCGCGAATAATCTGCGAACCGTCCGGCGCGCCGACCAGCGCACGCAGCCAGATTTCGCCATCAA                                                       <  SRR4302537.1916242/94‑1 (MQ=60)
                                    TCGGCATCTTGCGGCGCGCCGCGGCGTTCACCGCGAATAATCTGCGAACCGTCCGGCGCGCCGACCAGCGCACGCAGCCAGATTTCGCCATCAA                                                       <  SRR4302537.6191036/94‑1 (MQ=60)
                                     CGGCATCTTGCGGCGCGCCGCGGCGTTCACCGCGAATAATCTGCGAACCGTCCGGCGCGCCGACCAGCGCACGCAGCCAGATTTCGCCATCAAT                                                      >  SRR4302537.2962680/1‑94 (MQ=60)
                                     CGGCATCTTGCGGCGCGCCGCGGCGTTCACCGCGAATAATCTGCGAACCGTCCGGCGCGCCGACCAGCGCACGCAGCCAGATTTCGCCATCAAT                                                      <  SRR4302537.3034651/94‑1 (MQ=60)
                                     CGGCATCTGGCGGCGCGCCGCGGCGTTCACCGCGAATAATCTGCGAACCGTCCGGCGCGCCGACCAGCGCACGCAGCCAGATTTCGCCATCAAT                                                      <  SRR4302537.4640386/94‑1 (MQ=60)
                                       GCATCTTGCGGCGCGCCGCGGCGTTCACCGCGAATAATCTGCGAACCGTCCGGCGCGCCGACCAGCGCACGCAGCCAGATTTCGCCATCAATAA                                                    <  SRR4302537.3999036/94‑1 (MQ=60)
                                       GCATCTTGCGGCGCGCCGCGGCGTTCACCGCGAATAATCTGCGAACCGTCCGGCGCGCCGACCAGCGCACGCAGCCAGATTTCGCCATCAATAA                                                    <  SRR4302537.1482027/94‑1 (MQ=60)
                                       GCATCTTGCGGCGCGCCGCGGCGTTCACCGCGAATAATCTGCGAACCGTCCGGCGCGCCGACCAGCGCACGCAGCCAGATTTCGCCATCAATAA                                                    <  SRR4302537.6095876/94‑1 (MQ=60)
                                       GCATCTTGCGGCGCGCCGCGGCGTTCACCGCGAATAATCTGCGAACCGTCCGGCGCGCCGACCAGCGCACGCAGCCAGATTTCGCCATCAATAA                                                    <  SRR4302537.166662/94‑1 (MQ=60)
                                        CATCTTGCGGCGCGCCGCGGCGTTCACCGCGAATAATCTGCGAACCGTCCGGCGCGCCGACCAGCGCACGCAGCCAGATTTCGCCATCAATAAG                                                   >  SRR4302537.5354084/1‑94 (MQ=60)
                                        CATCTTGCGGCGCGCCGCGGCGTTCACCGCGAATAATCTGCGAACCGTCCGGCGCGCCGACCAGCGCACGCAGCCAGATTTCGCCATCAATAAG                                                   >  SRR4302537.1521384/1‑94 (MQ=60)
                                         ATCTTGCGGCGCGCCGCGGCGTTCACCGCGAATAATCTGCGAACCGTCCGGCGCGCCGACCAGCGCACGCAGCCAGATTTCGCCATCAATAAGC                                                  <  SRR4302537.3879303/94‑1 (MQ=60)
                                            TTGCGGCGCGCCGCGGCGTTCACCGCGAATAATCTGCGAACCGTCCGGCGCGCCGACCAGCGCACGCAGCCAGATTTCGCCATCAATAAGCTCG                                               <  SRR4302537.4831673/94‑1 (MQ=60)
                                            TTGCGGCGCGCCGCGGCGTTCACCGCGAATAATCTGCGAACCGTCCGGCGCGCCGACCAGCGCACGCAGCCAGATTTCGCCATCAATAAGCTCG                                               <  SRR4302537.670059/94‑1 (MQ=60)
                                            TTGCGGCGCGCCGCGGCGTTCACCGCGAATAATCTGCGAACCGTCCGGCGCGCCGACCAGCGCACGCAGCCAGATTTCGCCATCAATAAGCTCG                                               <  SRR4302537.1440879/94‑1 (MQ=60)
                                               CGGCGCGCCGCGGCGTTCACCGCGAATAATCTGCGAACCGTCCGGCGCGCCGACCAGCGCACGCAGCCAGATTTCGCCATCAATAAGCTCGGCG                                            <  SRR4302537.1414198/94‑1 (MQ=60)
                                                      CCGCGGCGTTCACCGCGAATAATCTGCGAACCGTCCGGCGCGCCGACCAGCGCACGCAGCCAGATTTCGCCATCAATAAGCTCGGCGTAGCTAC                                     <  SRR4302537.5921627/94‑1 (MQ=60)
                                                      CCGCGGCGTTCACCGCGAATAATCTGCGAACCGTCCGGCGCGCCGACCAGCGCACGCAGCCAGATTTCGCCATCAATAAGCTCGGCGTAGCTAC                                     <  SRR4302537.4785633/94‑1 (MQ=60)
                                                       CGCGGCGTTCACCGCGAATAATCTGCGAACCGTCCGGCGCGCCGACCAGCGCACGCAGCCAGATTTCGCCATCAATAAGCTCGGCGTAGCTACC                                    >  SRR4302537.1577388/1‑94 (MQ=60)
                                                       CGCGGCGTTCACCGCGAATAATCTGCGAACCGTCCGGCGCGCCGACCAGCGCACGCAGCCAGATTTCGCCATCAATAAGCTCGGCGTAGCTACC                                    >  SRR4302537.3224936/1‑94 (MQ=60)
                                                           GCGTTCACCGCGAATAATCTGCGAACCGTCCGGCGCGCCGACCAGCGCACGCAGCCAGATTTCGCCATCAATAAGCTCGGCGTAGCTACCAATT                                <  SRR4302537.4531930/94‑1 (MQ=60)
                                                           GCGTTCACCGCGAATAATCTGCGAACCGTCCGGCGCGCCGACCAGCGCACGCAGCCAGATTTCGCCATCAATAAGCTCGGCGTAGCTACCAATT                                <  SRR4302537.2378149/94‑1 (MQ=60)
                                                            CGTTCACCGCGAATAATCTGCGAACCGTCCGGCGCGCCGACCAGCGCACGCAGCCAGATTTCGCCATCAATAAGCTCGGCGTAGCTACCAATTG                               <  SRR4302537.2051144/94‑1 (MQ=60)
                                                            CGTTCACCGCGAATAATCTGCGAACCGTCCGGCGCGCCGACCAGCGCACGCAGCCAGATTTCGCCATCAATAAGCTCGGCGTAGCTACCAATTG                               >  SRR4302537.5606611/1‑94 (MQ=60)
                                                             GTTCACCGCGAATAATCTGCGAACCGTCCGGCGCGCCGACCAGCGCACGCAGCCAGATTTCGCCATCAATAAGCTCGGCGTAGCTACCAATTGG                              <  SRR4302537.1319565/94‑1 (MQ=60)
                                                             GTTCACCGCGAATAATCTGCGAACCGTCCGGCGCGCCGACCAGCGCACGCAGCCAGATTTCGCCATCAATAAGCTCGGCGTAGCTACCAATTGG                              >  SRR4302537.4258368/1‑94 (MQ=60)
                                                              TTCACCGCGAATAATCTGCGAACCGTCCGGCGCGCCGACCAGCGCACGCAGCCAGATTTCGCCATCAATAAGCTCGGCGTAGCTACCAATTGGC                             >  SRR4302537.2997005/1‑94 (MQ=60)
                                                              TTCACCGCGAATAATCTGCGAACCGTCCGGCGCGCCGACCAGCGCACGCAGCCAGATTTCGCCATCAATAAGCTCGGCGTAGCTACCAATTGGC                             <  SRR4302537.4854941/94‑1 (MQ=60)
                                                                CACCGCGAATAATCTGCGAACCGTCCGGCGCGCCGACCAGCGCACGCAGCCAGATTTCGCCATCAATAAGCTCGGCGTAGCTACCAATTGGCAC                           <  SRR4302537.6203366/94‑1 (MQ=60)
                                                                CACCGCGAATAATCTGCGAACCGTCCGGCGCGCCGACCAGCGCACGCAGCCAGATTTCGCCATCAATAAGCTCGGCGTAGCTACCAATTGGCAC                           <  SRR4302537.395777/94‑1 (MQ=60)
                                                                CACCGCGAATAATCTGCGAACCGTCCGGCGCGACGACCAGCGCACGCAGCCAGATTTCGCCATCAATAAGCTCGGCGTAGCTACCAATTGGCAC                           <  SRR4302537.3178470/94‑1 (MQ=60)
                                                                   CGCGAATAATCTGCGAACCGTCCGGCGCGCCGACCAGCGCACGCAGCCAGATTTCGCCATCAATAAGCTCGGCGTAGCTACCAATTGGCACCTG                        <  SRR4302537.5261862/94‑1 (MQ=60)
                                                                   CGCGAATAATCTGCGAACCGTCCGGCGCGCCGACCAGCGCACGCAGCCAGATTTCGCCATCAATAAGCTCGGCGTAGCTACCAATTGGCACCTG                        >  SRR4302537.4515611/1‑94 (MQ=60)
                                                                   CGCGAATAATCTGCGAACCGTCCGGCGCGCCGACCAGCGCACGCAGCCAGATTTCGCCATCAATAAGCTCGGCGTAGCTACCAATTGGCACCTG                        <  SRR4302537.3747919/94‑1 (MQ=60)
                                                                    GCGAATAATCTGCGAACCGTCCGGCGCGCCGACCAGCGCACGCAGCCAGATTTCGCCATCAATAAGCTCGGCGTAGCTACCAATTGGCACCTGA                       >  SRR4302537.1879973/1‑94 (MQ=60)
                                                                     CGAATAATCTGCGAACCGTCCGGTGCGCCGACCAGCGCACGCAGCCAGATTTCGCCATCAATAAGCTCGGCGTAGCTACCAATTGGCACCTGAC                      <  SRR4302537.3348732/94‑1 (MQ=60)
                                                                     CGAATAATCTGCGAACCGTCCGGCGCGCCGACCAGCGCACGCAGCCAGATTTCGCCATCAATAAGCTCGGCGTAGCTACCAATTGGCACCTGAC                      <  SRR4302537.3816439/94‑1 (MQ=60)
                                                                     CGAATAATCTGCGAACCGTCCGGCGCGCCGACCAGCGCACGCAGCCAGATTTCGCCATCAATAAGCTCGGCGTAGCTACCAATTGGCACCTGAC                      <  SRR4302537.2359314/94‑1 (MQ=60)
                                                                     CGAATAATCTGCGAACCGTCCGGCGCGCCGACCAGCGCACGCAGCCAGATTTCGCCATCAATAAGCTCGGCGTAGCTACCAATTGGCACCTGAC                      >  SRR4302537.6494550/1‑94 (MQ=60)
                                                                      GAATAATCTGCGAACCGTCCGGCGCGCCGACCAGCGCACGCAGCCAGATTTCGCCATCAATAAGCTCGGCGTAGCTACCAATTGGCACCTGACA                     <  SRR4302537.5617623/94‑1 (MQ=60)
                                                                        ATAATCTGCGAACCGTCCGGCGCGCCGACCAGCGCACGCAGCCAGATTTCGCCATCAATAAGCTCGGCGTAGCTACCAATTGGCACCTGACATC                   >  SRR4302537.2725244/1‑94 (MQ=60)
                                                                         TAATCTGCGAACCGTCCGGCGCGCCGACCAGCGCACGCAGCCAGATTTCGCCATCAATAAGCTCGGCGTAGCTACCAATTGGCACCTGACATCC                  >  SRR4302537.3806519/1‑94 (MQ=60)
                                                                           ATCTGCGAACCGTCCGGCGCGCCGACCAGCGCACGCAGCCAGATTTCGCCATCAATAAGCTCGGCGTAGCTACCAATTGGCACCTGACATCCGC                >  SRR4302537.5707433/1‑94 (MQ=60)
                                                                           ATCTGCGAACCGTCCGGCGCGCCGACCAGCGCACGCAGCCAGATTTCGCCATCAATAAGCTCGGCGTAGCTACCAATTGGCACCTGACATCCGC                >  SRR4302537.624539/1‑94 (MQ=60)
                                                                             CTGCGAACCGTCCGGCGCGCCGACCAGCGCACGCAGCCAGATTTCGCCATCAATAAGCTCGGCGTAGCTACCAATTGGCACCTGACATCCGCCT              <  SRR4302537.5406071/94‑1 (MQ=60)
                                                                             CTGCGAACCGTCCGGCGCGCCGACCAGCGCACGCAGCCAGATTTCGCCATCAATAAGCTCGGCGTAGCTACCAATTGGCACCTGACATCCGCCT              <  SRR4302537.4093770/94‑1 (MQ=60)
                                                                             CTGCGAACCGTCCGGCGCGCCGACCAGCGCACGCAGCCAGATTTCGCCATCAATAAGCTCGGCGTAGCTACCAATTGGCACCTGACATCCGCCT              >  SRR4302537.6456127/1‑94 (MQ=60)
                                                                               GCGAACCGTCCGGCGCGCCGACCAGCGCACGCAGCCAGATTTCGCCATCAATAAGCTCGGCGTAGCTACCAATTGGCACCTGACATCCGCCTTC            >  SRR4302537.1568628/1‑94 (MQ=60)
                                                                                  AACCGTCCGGCGCGCCGACCAGCGCACGCAGCCAGATTTCGCCATCAATAAGCTCGGCGTAGCTACCAATTGGCACCTGACATCCGCCTTCGAG         <  SRR4302537.1930137/94‑1 (MQ=60)
                                                                                  AACCGTCCGGCGCGCCGACCAGCGCACGCAGCCAGATTTCGCCATCAATAAGCTCGGCGTAGCTACCAATTGGCACCTGACATCCGCCTTCGAG         <  SRR4302537.3750431/94‑1 (MQ=60)
                                                                                    CCGTCCGGCGCGCCGACCAGCGCACGCAGCCAGATTTCGCCATCAATAAGCTCGGCGTAGCTACCAATTGGCACCTGACATCCGCCTTCGAGAC       >  SRR4302537.3837461/1‑94 (MQ=60)
                                                                                      GTCCGGCGCGCCGACCAGCGCACGCAGCCAGATTTCGCCATCAATAAGCTCGGCGTAGCTACCAATTGGCACCTGACATCCGCCTTCGAGACGG     >  SRR4302537.1208871/1‑94 (MQ=60)
                                                                                      GTCCGGCGCGCCGACCAGCGCACGCAGCCAGATTTCGCCATCAATAAGCTCGGCGTAGCTACCAATTGGCACCTGACATCCGCCTTCGAGACGG     >  SRR4302537.1098823/1‑94 (MQ=60)
                                                                                       TCCGGCGCGCCGACCAGCGCACGCAGCCAGATTTCGCCATCAATAAGCTCGGCGTAGCTACCAATTGGCACCTGACATCCGCCTTCGAGACGGG    <  SRR4302537.3232343/94‑1 (MQ=60)
                                                                                        CCGGCGCGCCGACCAGCGCACGCAGCCAGATTTCGCCATCAATAAGCTCGGCGTAGCTACCAATTGGCACCTGACATCCGCCTTCGAGACGGGT   <  SRR4302537.2212383/94‑1 (MQ=60)
                                                                                         CGGCGCGCCGACCAGCGCACGCAGCCAGATTTCGCCATCAATAAGCTCGGCGTAGCTACCAATTGGCACCTGACATCCGCCTTCGAGACGGGTA  >  SRR4302537.5029132/1‑94 (MQ=60)
                                                                                             |                                                                                         
TTCAGTAGCTCTTCTGCCAGCGAAATCCCCATTTGTTCGGCATCTTGCGGCGCACCGCGGCGTTCACCGCGAATAATCTGCGAACCGTCCGGCCGGCCGACCAGCGCACGCAGCCAGATTTCGCCATCAATAAGCTCGGCGTAGCTACCAATTGGCACCTGACATCCGCCTTCGAGACGGGTA  >  NC_000913/3987493‑3987675

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 6 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑