Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I2 R1 882 254.4 12957588 98.0% 12698436 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,993,410 T→C M72T (ATG→ACG)  yigA → hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,993,4100TC97.7% 717.1 / 4.2 214M72T (ATG→ACG) yigAhypothetical protein
Reads supporting (aligned to +/- strand):  ref base T (0/5);  new base C (123/86);  total (123/91)
Fisher's exact test for biased strand distribution p-value = 1.30e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.46e-01

Only 100 of 220 total aligned reads displayed.

GTGTGCCGCATCCGGTACGCGGCACCGTTTCGTTGGTCGAGTGGCACATGGCCCGCGCACGTAATCATATTCATGTTCTGGAAGAGAACATGGCGCTGTTGATGGAACAGGCTATCGCCAACGAAGGCCTGTTTTATCGCCTACTCTACCTGCAGCGCAGTCTCACCGCCGCCAGCAGTCTCG  >  NC_000913/3993320‑3993502
                                                                                          |                                                                                            
gtgtGCCGCATCCGGTACGCGGCACCGTTTCGTTGGTCGAGTGGCACATGGCCCGCGCACGTAATCATATTCATGTTCTGGAAGAGAACACgg                                                                                            <  1:1474120/93‑1 (MQ=255)
 tgtgCCGCATCCGGTACGCGGCACCGTTTCGTTGGTCGAGTGGCACATGGCCCGCGCACGTAATCATATTCATGTTCTGGAAGAGAACACGgc                                                                                           <  2:4472310/93‑1 (MQ=255)
 tgtgCCGCATCCGGTACGCGGCACCGTTTCGTTGGTCGAGTGGCACATGGCCCGCGCACGTAATCATATTCATGTTCTGGAAGAGAACACGgc                                                                                           >  2:4594153/1‑93 (MQ=255)
   tgCCGCATCCGGTACGCGGCACCGTTTCGTTGGTCGAGTGGCACATGGCCCGCGCACGTAATCATATTCATGTTCTGGAAGAGAACACGgcgc                                                                                         <  1:4346168/93‑1 (MQ=255)
     ccGCATCCGGTACGCGGCACCGTTTCGTTGGTCGAGTGGCACATGGCCCGCGCACGTAATCATATTCATGTTCTGGAAGAGAACACGGCGCTg                                                                                       >  2:3929722/1‑93 (MQ=255)
      cGCATCCGGTACGCGGCACCGTTTCGTTGGTCGAGTGGCACATGGCCCGCGCACGTAATCATATTCATGTTCTGGAAGAGAACACGGCGCTGt                                                                                      >  2:1016989/1‑93 (MQ=255)
         aTCCGGTACGCGGCACCGTTTCGTTGGTCGAGTGGCACATGGCCCGCGCACGTAATCATATTCATGTTCTGGAAGAGAACACGGCGCTGTTGa                                                                                   >  2:4463679/1‑93 (MQ=255)
         aTCCGGTACGCGGCACCGTTTCGTTGGTCGAGTGGCACATGGCCCGCGCACGTAATCATATTCATGTTCTGGAAGAGAACACGGCGCTGTTGa                                                                                   <  1:210824/93‑1 (MQ=255)
         aTCCGGTACGCGGCACCGTTTCGTTGGTCGAGTGGCACATGGCCCGCGCACGTAATCATATTCATGTTCTGGAAGAGAACACGGCGCTGTTGa                                                                                   <  1:4543549/93‑1 (MQ=255)
         aTCCGGTACGCGGCACCGTTTCGTTGGTCGAGTGGCACATGGCCCGCGCACGTAATCATATTCATGTTCTGGAAGAGAACACGGCGCTGTTGa                                                                                   >  2:2473057/1‑93 (MQ=255)
         aTCCGGTACGCGGCACCGTTTCGTTGGTCGAGTGGCACATGGCCCGCGCACGTAATCATATTCATGTTCTGGAAGAGAACACGGCGCTGTTGa                                                                                   <  2:271244/93‑1 (MQ=255)
          tCCGGTACGCGGCACCGTTTCGTTGGTCGAGTGGCACATGGCCCGCGCACGTAATCATATTCATGTTCTGGAAGAGAACACGGCGCTGTTGAt                                                                                  >  1:4152094/1‑93 (MQ=255)
           ccGGTACGCGGCACCGTTTCGTTGGTCGAGTGGCACATGGCCCGCGCACGTAATCATATTCATGTTCTGGAAGAGAACACGGCGCTGTTGATg                                                                                 >  1:2668460/1‑93 (MQ=255)
           ccGGTACGCGGCACCGTTTCGTTGGTCGAGTGGCACATGGCCCGCGCACGTAATCATATTCATGTTCTGGAAGAGAACACGGCGCTGTTGATg                                                                                 <  2:6257936/93‑1 (MQ=255)
           ccGGTACGCGGCACCGTTTCGTTGGTCGAGTGGCACATGGCCCGCGCACGTAATCATATTCATGTTCTGGAAGAGAACACGGCGCTGTTGATg                                                                                 <  2:6391478/93‑1 (MQ=255)
            cGGTACGCGGCACCGTTTCGTTGGTCGAGTGGCACATGGCCCGCGCACGTAATCATATTCATGTTCTGGAAGAGAACACGGCGCTGTTGATgg                                                                                <  2:6476875/93‑1 (MQ=255)
              gTACGCGGCACCGTTTCGTTGGTCGAGTGGCACATGGCCCGCGCACGTAATCATATTCATGTTCTGGAAGAGAACACGGCGCTGTTGATGGaa                                                                              <  2:3986828/93‑1 (MQ=255)
               tACGCGGCACCGTTTCGTTGGTCGAGTGGCACATGGCCCGCGCACGTAATCATATTCATGTTCTGGAAGAGAACACGGCGCTGTTGATGGAAc                                                                             >  1:1040499/1‑93 (MQ=255)
                aCGCGGCACCGTTTCGTTGGTCGAGTGGCACATGGCCCGCGCACGTAATCATATTCATGTTCTGGAAGAGAACACGGCGCTGTTGATGGAACa                                                                            <  1:2611046/93‑1 (MQ=255)
                 cgcgGCACCGTTTCGTTGGTCGAGTGGCACATGGCCCGCGCACGTAATCATATTCATGTTCTGGAAGAGAACACGGCGCTGTTGATGGAACAg                                                                           <  2:5663816/93‑1 (MQ=255)
                   cgGCACCGTTTCGTTGGTCGAGTGGCACATGGCCCGCGCACGTAATCATATTCATGTTCTGGAAGAGAACACGGCGCTGTTGATGGAACAGGc                                                                         >  2:4798841/1‑93 (MQ=255)
                   cgGCACCGTTTCGTTGGTCGAGTGGCACATGGCCCGCGCACGTAATCATATTCATGTTCTGGAAGAGAACACGGCGCTGTTGATGGAACAGGc                                                                         <  2:1333908/93‑1 (MQ=255)
                   cgGCACCGTTTCGTTGGTCGAGTGGCACATGGCCCGCGCACGTAATCATATTCATGTTCTGGAAGAGAACACGGCGCTGTTGATGGAACAGGc                                                                         <  1:727036/93‑1 (MQ=255)
                   cgGAACCGTTTCGTTGGTCGAGTGGCACATGGCCCGCGCACGTAATCATATTCATGTTCGGGAAGAGAACACGGCGCTGTTGATGGAACAGGc                                                                         <  2:4537332/93‑1 (MQ=255)
                      gactgTTTCGTTGGTCGAGTGGCACAGGGCCCGCGCACGTAATCATATTCATGTTCGGGAAGAGAACACGGCGCTGTTGATGGAACAGGCTAt                                                                      <  1:6189271/89‑1 (MQ=255)
                       aCCGTTTCGTTGGTCGAGTGGCACATGGCCCGCGCACGTAATCATATTCATGTTCTGGAAGAGAACACGGCGCTGTTGATGGAACAGGATATc                                                                     >  1:151330/1‑93 (MQ=255)
                       aCCGTTTCGTTGGTCGAGTGGCACATGGCCCGCGCACGTAATCATATTCATGTTCTGGAAGAGAACACGGCGCTGCTGATGGAACAGGCTATc                                                                     >  2:5446085/1‑93 (MQ=255)
                        ccGTTTCGTTGGTCGAGTGGCACATGGCCCGCGCACGTAATCATATTCATGTTCTGGAAGAGAACACGGCGCTGTTGATGGAACAGGCTATCg                                                                    <  2:3072213/93‑1 (MQ=255)
                        ccGTTTCGTTGGTCGAGTGGCACATGGCCCGCGCACGTAATCATATTCATGTTCTGGAAGAGAACACGGCGCTGTTGATGGAACAGGCTATCg                                                                    >  1:768881/1‑93 (MQ=255)
                         cGTTTCGTTGGTCGAGTGGCACATGGCCCGCGCACGTAATCATATTCATGTTCTGGAAGAGAACACGGCGCTGTTGATGGAACAGGCTATCGc                                                                   >  2:6087958/1‑93 (MQ=255)
                            ttCGTTGGTCGAGTGGCACATGGCCCGCGCACGTAATCATATTCATGTTCTGGAAGAGAACACGGCGCTGTTGATGGAACAGGCTATCGCCaa                                                                <  1:2147115/93‑1 (MQ=255)
                            ttCGTTGGTCGAGTGGCACATGGCCCGCGCACGTAATCATATTCATGTTCTGGAAGAGAACACGGCGCTGTTGATGGAACAGGCTATCGCCaa                                                                <  2:5067525/93‑1 (MQ=255)
                             tCGTTGGTCGAGTGGCACATGGCCCGCGCACGTAATCATATTCATGTTCTGGAAGAGAACACGGCGCTGTTGATGGAACAGGCTATCGCCAAc                                                               >  1:1990290/1‑93 (MQ=255)
                               gTTGGTCGAGTGGCACATGGCCCGCGCACGTAATCATATTCATGTTCTGGAAGAGAACACGGCGCTGTTGATGGAACAGGCTATCGCCAACGa                                                             >  1:5422902/1‑93 (MQ=255)
                                ttGGTCGAGTGGCACATGGCCCGCGCACGTAATCATATTCATGTTCTGGAAGAGAACACGGCGCTGTTGATGGAACAGGCTATCGCCAACGaa                                                            >  2:659667/1‑93 (MQ=255)
                                    tCGAGTGGCACATGGCCCGCGCACGTAATCATATTCATGTTCTGGAAGAGAACATGGCGCTGTTGATGGAACAGGCTATCGCCAACGAAGGcc                                                        <  2:1888946/93‑1 (MQ=255)
                                     cGAGTGGCACATGGCCCGCGCACGTAATCATATTCATGTTCTGGAAGAGAACACGGCGCTGTTGATGGAACAGGCTATCGCCAACGAAGGCCt                                                       <  1:1920596/93‑1 (MQ=255)
                                      gAGTGGCACATGGCCCGCGCACGTAATCATATTCATGTTCTGGAAGAGAACACGGCGCTGTTGATGGAACAGGCTATCGCCAACGAAGGCCTg                                                      >  1:110490/1‑93 (MQ=255)
                                      gAGTGGCACATGGCCCGCGCACGTAATCATATTCATGTTCTGGAAGAGAACACGGCGCTGTTGATGGAACAGGCTATCGCCAACGAAGGCCTg                                                      <  2:144570/93‑1 (MQ=255)
                                        gTGGCACATGGCCCGCGCACGTAATCATATTCATGTTCTGGAAGAGAACACGGCGCTGTTGATGGAACAGGCTATCGCCAACGAAGGCCTGtt                                                    <  1:5901924/93‑1 (MQ=255)
                                         tGGCACATGGCCCGCGCACGTAATCATATTCATGTTCTGGAAGAGAACACGGCGCTGTTGATGGAACAGGCTATCGCCAACGAAGGCCTGttt                                                   >  1:4032856/1‑93 (MQ=255)
                                          ggCACATGGCCCGCGCACGTAATCATATTCATGTTCTGGAAGAGAACACGGCGCTGTTGATGGAACAGGCTATCGCCAACGAAGGCCTGtttt                                                  <  2:537639/93‑1 (MQ=255)
                                           gCACATGGCCCGCGCACGTAATCATATTCATGTTCTGGAAGAGAACACGGCGCTGTTGATGGAACAGGCTATCGCCAACGAAGGCCTGTTTTa                                                 >  2:5127633/1‑93 (MQ=255)
                                            cacaTGGCCCGCGCACGTAATCATATTCATGTTCTGGAAGAGAACACGGCGCTGTTGATGGAACAGGCTATCGCCAACGAAGGCCTGTTTTAt                                                >  1:973613/1‑93 (MQ=255)
                                            cacaTGGCCCGCGCACGTAATCATATTCATGTTCTGGAAGAGAACACGGCGCTGTTGATGGAACAGGCTATCGCCAACGAAGGCCTGTTTTAt                                                <  2:385073/93‑1 (MQ=255)
                                             acaTGGCCCGCGCACGTAATCATATTCATGTTCTGGAAGAGAACACGGCGCTGTTGATGGAACAGGCTATCGCCAACGAAGGCCTGTTTTATc                                               >  1:5745754/1‑93 (MQ=255)
                                             acaTGGCCCGCGCACGTAATCATATTCATGTTCTGGAAGAGAACACGGCGCTGATGATGGAACAGGCTATCGCCAACGAAGGCCTGTTTTATc                                               <  2:1908160/93‑1 (MQ=255)
                                               aTGGCCCGCGCACGTAATCATATTCATGTTCTGGAAGAGAACACGGCGCTGTTGATGGAACAGGCTATCGCCAACGAAGGCCTGTTTTATCGc                                             >  1:1591850/1‑93 (MQ=255)
                                                  gCCCGCGCACGTAATCATATTCATGTTCTGGAAGAGAACACGGCGCTGTTGATGGAACAGGCTATCGCCAACGAAGGCCTGTTTTATCGCCTa                                          >  2:2681758/1‑93 (MQ=255)
                                                  gCCCGCGCACGTAATCATATTCATGTTCTGGAAGAGAACACGGCGCTGTTGATGGAACAGGCTATAGCCAACGAAGGCCTGTTTTATCGCCTa                                          <  2:5267560/93‑1 (MQ=255)
                                                     cgcgCACGTAATCATATTCATGTTCCGGAAGAGAACACGGCGCTGTTGATGGAACAGGCTATCGCCAACGAAGGCCTGTTTTATCGCCTActc                                       <  2:3720862/93‑1 (MQ=255)
                                                      gcgcACGTAATCATATTCATGTTCTGGAAGAGAACACGGCGCTGTTGATGGAACAGGCTATCGCCAACGAAGGCCTGTTTTATCGCCTActct                                      >  1:4221065/1‑93 (MQ=255)
                                                        gcACGTAATCATATTCATGTTCTGGAAGAGAACACGGCGCTGTTGATGGAACAGGCTATCGCCAACGAAGGCCTGTTTTATCGCCTACTCTAc                                    >  1:1801491/1‑93 (MQ=255)
                                                         cACGTAATCATATTCATGTTCTGGAAGAGAACACGGCGCTGTTGATGGAACAGGCTATCGCCAACGAAGGCCTGTTTTATCGCCTACTCTAcc                                   >  2:6038447/1‑93 (MQ=255)
                                                          aCGTAATCATATTCATGTTCTGGAAGAGAACACGGCGCTGTTGATGGAACAGGCTATCGCCAACGAAGGCCTGTTTTATCGCCTACTCTACCt                                  <  2:3600894/93‑1 (MQ=255)
                                                           cGTAATCATATTCATGTTCTGGAAGAGAACACGGCGCTGTTGATGGAACAGGCTATCGCCAACGAAGGCCTGTTTTATCGCCTACTCTACCTg                                 >  2:335097/1‑93 (MQ=255)
                                                            gTAATCATATTCATGTTCTGGAAGAGAACACGGCGCTGTTGATGGAACAGGCTATCGCCAAGGAAGGCCTGTTTTATCGCCTAGTCTACCTGc                                >  1:5803444/1‑93 (MQ=255)
                                                            gTAATCATATTCATGTTCTGGAAGAGAACACGGCGCTGTTGATGGAACAGGCTATCGCCAACGAAGGCCTGTTTTATCGCCTACTCTACCTga                                >  2:2079340/1‑92 (MQ=255)
                                                            gTAATCATATTCATGTTCTGGAAGAGAACACGGCGCTGTTGATGGAACAGGCTATCGCCAACGAAGGCCTGTTTTATCGCCTACTCTACCTGc                                >  1:5351677/1‑93 (MQ=255)
                                                            gTAATCATATTCATGTTCTGGAAGAGAACACGGCGCTGTTGATGGAACAGGCTATCGCCAACGAAGGCCTGTTTTATCGCCTACTCTACCTGc                                <  1:4910676/93‑1 (MQ=255)
                                                            gTAATCATATTCATGTTCTGGAAGAGAACACGGCGCTGTTGATGGAACAGGCTATCGCCAACGAAGGCCTGTTTTATCGCCTACTCTACCTGc                                <  2:3249495/93‑1 (MQ=255)
                                                             tAATCATATTCATGTTCTGGAAGAGAACACGGCGCTGTTGATGGAACAGGCTATCGCCAACGAAGGCCTGTTTTATCGCCTACTCTACCTGCa                               >  2:1997873/1‑93 (MQ=255)
                                                             tAATCATATTCATGTTCTGGAAGAGAACACGGCGCTGTTGATGGAACAGGCTATCGCCAACGAAGGCCTGTTTTATCGCCTACTCTACCTGCa                               <  1:1446321/93‑1 (MQ=255)
                                                              aaTCATATTCATGTTCTGGAAGAGAACACGGCGCTGTTGATGGAACAGGCTATCGCCAACGAAGGCCTGTTTTATCGCCTACTCTACCTGCAg                              >  1:5994581/1‑93 (MQ=255)
                                                               aTCATATTCATGTTCTGGAAGAGAACACGGCGCTGTTGATGGAACAGGCTATCGCCAACGAAGGCCTGTTTTATCGCCTACTCTACCTGCAgc                             >  1:2454560/1‑93 (MQ=255)
                                                               aTCATATTCATGTTCTGGAAGAGAACACGGCGCTGTTGATGGAACAGGCTATCGCCAACGAAGGCCTGTTTTATCGCCTACTCTACCTGCAgc                             <  1:2898233/93‑1 (MQ=255)
                                                                tCATATTCATGTTCTGGAAGAGAACACGGCGCTGTTGATGGAACAGGCTATCGCCAACGAAGGCCTGTTTTATCGCCTACTCTACCTGCAgcg                            <  2:5218957/93‑1 (MQ=255)
                                                                tCATATTCATGTTCTGGAAGAGAACACGGCGCTGTTGATGGAACAGGCTATCGCCAACGAAAGCCTGTTTTATCGCCTACTCTACCTGCAgcg                            >  2:3447948/1‑93 (MQ=255)
                                                                tCATATTCATGTTCTGGAAGAGAACACGGCGCTGTTGATGGAAAAGGCTATCGCCAACGAAGGCCTGTTTTATCGCCTACTCTACCTGCAgcc                            >  1:640907/1‑92 (MQ=255)
                                                                 cATATTCATGTTCTGGAAGAGAACACGGCGCTGTTGATGGAACAGGCTATCGCCAACGAAGGCCTGTTTTATCGCCTACTCTACCTGCAgcgc                           >  1:6063801/1‑93 (MQ=255)
                                                                  atatTCATGTTCTGGAAGAGAACACGGCGCTGTTGATGGAACAGGCTATCGCCAACGAAGGCCTGTTTTATCGCCTACTCTACCTGCAGCGCa                          >  2:2910733/1‑93 (MQ=255)
                                                                  atatTCATGTTCTGGAAGAGAACACGGCGCTGTTGATGGAACAGGCTATCGCCAACGAAGGCCTGTTTTATCGCCTACTCTACCTGCAGCGCa                          >  2:4224175/1‑93 (MQ=255)
                                                                  atatTCATGTTCTGGAAGAGAACACGGCGCTGTTGATGGAACAGGCTATCGCCAACGAAGGCCTGTTTTATCGCCTACTCTACCTGCAGCGCa                          >  2:119127/1‑93 (MQ=255)
                                                                   tatTCATGTTCTGGAAGAGAACACGGCGCTGTTGATGGAACAGGCTATCGCCAACGAAGGCCTGTTTTATCGCCTACTCTACCTGCAGCGCAg                         >  1:1355622/1‑93 (MQ=255)
                                                                   tatTCATGTTCTGGAAGAGAACACGGCGCTGTTGATGGAACAGGCTATCGCCAACGAAGGCCTGTTTTATCGCCTACTCTACCTGCAGCGCAg                         <  1:1408812/93‑1 (MQ=255)
                                                                   tatTCATGTTCTGGAAGAGAACACGGCGCTGTTGATGGAACAGGCTATCGCCAACGAAGGCCTGTTTTATCGCCTACTCTACCTGCAGCGCAg                         >  1:5651411/1‑93 (MQ=255)
                                                                   tatTCATGTTCTGGAAGAGAACACGGCGCTGTTGATGGAACAGGCTATCGCCAACGAAGGCCTGTTTTATCGCCTACTCTACCTGCAGCGCAg                         <  1:1161369/93‑1 (MQ=255)
                                                                   tatTCATGTTCTGGAAGAGAACACGGCGCTGTTGATGGAACAGGCTATCGCCAACGAAGGCCTGTTTTATCGCCTACTCTACCTGCAGCGCAg                         <  2:1510898/93‑1 (MQ=255)
                                                                     ttCATGTTCTGGAAGAGAACACGGCGCTGTTGATGGAACAGGCTATCGCCAACGAAGGCCTGTTTTATCGCCTACTCTACCTGCAGCGCAGtc                       <  1:3795087/93‑1 (MQ=255)
                                                                     ttCATGTTCTGGAAGAGAACACGGCGCTGTGGATGGAACAGGCTATCGCCAACGAAGGCCTGTTGTATCGCCTACTCTACCTGCAGCGCAGtc                       <  1:3305318/93‑1 (MQ=255)
                                                                      tCATGTTCTGGAAGAGAACACGGCGCTGTTGATGGAACAGGCTATCGCCAACGAAGGCCTGTTTTATCGCCTACTCTACCTGCAGCGCAGtct                      <  2:4022092/93‑1 (MQ=255)
                                                                      tCATGTTCTGGAAGAGAACACGGCGCTGTTGATGGAACAGGCTATCGCCAACGAAGGCCTGTTTTATCGCCTACTCTACCTGCAGCGCAGtct                      >  2:3158460/1‑93 (MQ=255)
                                                                      tCATGTTCTGGAAGAGAACACGGCGCTGTTGATGGAACAGGCTATCGCCAACGAAGGCCTGTTTTATCGCCTACTCTACCTGCAGCGCAGtcc                      >  1:4164451/1‑92 (MQ=255)
                                                                       cATGTTCTGGAAGAGAACACGGCGCTGTTGATGGAACAGGCTATCGCCAACGAAGGCCTGTTTTATCGCCTACTCTACCTGCAGCGCAGtctc                     >  2:2824490/1‑93 (MQ=255)
                                                                        aTGTTCTGGAAGAGAACACGGCGCTGTTGATGGAACAGGCTATCGCCAACGAAGGCCTGTTTTATCGCCTACTCTACCTGCAGCGCAGTCTCa                    >  2:2103356/1‑93 (MQ=255)
                                                                        aTGTTCTGGAAGAGAACACGGCGCTGTTGATGGAACAGGCTATCGCCAACGAAGGCCTGTTTTATCGCCTACTCTACCTGCAGCGCAGTCTCa                    >  1:1331846/1‑93 (MQ=255)
                                                                        aTGTTCTGGAAGAGAACACGGCGCTGTTGATGGAACAGGCTATCGCCAACGAAGGCCTGTTTTATCGCCTACTCTACCTGCAGCGCAGTCTCa                    >  1:3465873/1‑93 (MQ=255)
                                                                          gTTCTGGAAGAGAACACGGCGCTGTTGATGGAACAGGCTATCGCCAACGAAGGCCTGTTTTATCGCCTACTCTACCTGCAGCGCAGTCTCAcc                  >  1:256007/1‑93 (MQ=255)
                                                                          gTTCTGGAAGAGAACACGGCGCTGTTGATGGAACAGGCTATCGCCAACGAAGGCCTGTTTTATCGCCTACTCTACCTGCAGCGCAGTCTCAcc                  <  2:6263780/93‑1 (MQ=255)
                                                                           ttCTGGAAGAGAACATGGCGCTGTTGATGGAACAGGCTATCGCCAACGAAGGCCTGTTTTATCGCCTACTCTACCTGCAGCGCAGTCTCAccg                 <  2:5001776/93‑1 (MQ=255)
                                                                           ttCTGGAAGAGAACACGGCGCTGTTGATGGAACAGGCTATCGCCAACGAAGGCCTGTTTTATCGCCTACTCTACCTGCAGCGCAGTCTCAccc                 >  1:6357041/1‑92 (MQ=255)
                                                                              tGGAAGAGAACACGGCGCTGTTGATGGAACAGGCTATCGCCAACGAAGGCCTGTTTTATCGCCTACTCTACCTGCAGCGCAGTCTCAccgccg              >  1:6047843/1‑93 (MQ=255)
                                                                               ggAAGAGAACACGGCGCTGTTGATGGAACAGGCTATCGCCAACGAAGGCCTGTTTTATTGCCTACTCTACCTGCAGCGCAGTCTCACcgccgc             >  1:4354720/1‑93 (MQ=255)
                                                                                gAAGAGAACACGGCGCTGTTGATGGAACAGGCTATCGCCAACGAAGGCCTGTTTTATCGCCTACTCTACCTGCAGCGCAGTCTCACCgccgcc            >  1:3709866/1‑93 (MQ=255)
                                                                                gAAGAGAACACGGCGCTGTTGATGGAACAGGCTATCGCCAACGAAGGCCTGTTTTATCGCCTACTCTACCTGCAGCGCAGTCTCACCgccgcc            >  2:1386583/1‑93 (MQ=255)
                                                                                 aaGAGAACACGGCGCTGTTGATGGAACAGGCTATCGCCAACGAAGGCCTGTTTTATCGCCTACTCTACCTGCAGCGCAGTCTCACCGCCGCca           <  1:650915/93‑1 (MQ=255)
                                                                                    agaACACGGCGCTGTTGATGGAACAGGCTATCGCCAACGAAGGCCTGTTTTATCGCCTACTCTACCTGCAGCGCAGTCTCACCGCCGCcagca        <  2:1654341/93‑1 (MQ=255)
                                                                                    agaACACGGCGCTGTTGATGGAACAGGCTATCGCCAACGAAGGCCTGTTTTATCGCCTACTCTACCTGCAGCGCAGTCTCACCGCCGCcagca        >  2:4919718/1‑93 (MQ=255)
                                                                                     gaACACGGCGCTGTTGATGGAACAGGCTATCGCCAACGAAGGCCTGTTTTATCGCCTACTCTACCTGCAGCGCAGTCTCACCGCCGCcagcag       >  2:2425236/1‑93 (MQ=255)
                                                                                      aaCACGGCGCTGTTGATGGAACAGGCTATCGCCAACGAAGGCCTGTTTTATCGCCTACTCTACCTGCAGCGCAGTCTCACCGCCGCCAGCAGt      >  2:5900608/1‑93 (MQ=255)
                                                                                          |                                                                                            
GTGTGCCGCATCCGGTACGCGGCACCGTTTCGTTGGTCGAGTGGCACATGGCCCGCGCACGTAATCATATTCATGTTCTGGAAGAGAACATGGCGCTGTTGATGGAACAGGCTATCGCCAACGAAGGCCTGTTTTATCGCCTACTCTACCTGCAGCGCAGTCTCACCGCCGCCAGCAGTCTCG  >  NC_000913/3993320‑3993502

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
TACGTGTGCCGCATCCGGTACGCGGCACCGTTTCGTTGGTCGAGTGGCACATGGCCCGCGCACGTAATCATATTCATGTTCTGGAAGAGAACATGGCGCTGTTGATGGAACAGGCTATCGCCAACGAAGGCCTGTTTTATCGCCTACTCTACCTGCAGCGCAGTCTCACCGCCGCCAGCAGTCTCG  >  NC_000913/3993317‑3993502
                                                                                             |                                                                                            
TACGTGTGCCGCATCCGGTACGCGGCACCGTTTCGTTGGTCGAGTGGCACATGGCCCGCGCACGTAATCATATTCATGTTCTGGAAGAGAACAC                                                                                              <  SRR4302537.3707494/94‑1 (MQ=60)
 ACGTGTGCCGCATCCGGTACGCGGCACCGTTTCGTTGGTCGAGTGGCACATGGCCCGCGCACGTAATCATATTCATGTTCTGGAAGAGAACACG                                                                                             <  SRR4302537.2386983/94‑1 (MQ=60)
 ACGTGTGCCGCATCCGGTACGCGGCACCGTTTCGTTGGTCGAGTGGCACATGGCCCGCGCACGTAATCATATTCATGTTCTGGAAGAGAACACG                                                                                             <  SRR4302537.5635295/94‑1 (MQ=60)
  CGTGTGCCGCATCCGGTACGCGGCACCGTTTCGTTGGTCGAGTGGCACATGGCCCGCGCACGTAATCATATTCATGTTCTGGAAGAGAACACGG                                                                                            <  SRR4302537.1535333/94‑1 (MQ=60)
  CGTGTGCCGCATCCGGTACGCGGCACCGTTTCGTTGGTCGAGTGGCACATGGCCCGCGCACGTAATCATATTCATGTTCTGGAAGAGAACACGG                                                                                            <  SRR4302537.288751/94‑1 (MQ=60)
  CGTGTGCCGCATCCGGTACGCGGCACCGTTTCGTTGGTCGAGTGGCACATGGCCCGCGCACGTAATCATATTCATGTTCTGGAAGAGAACACGG                                                                                            <  SRR4302537.6041149/94‑1 (MQ=60)
  CGTGTGCCGCATCCGGTACGCGGCACCGTTTCGGTGGTCGAGTGGCACATGGCGCGCGCACGTAATCATATTCATGTTCTGGAAGAGAACACGG                                                                                            <  SRR4302537.3384578/94‑1 (MQ=60)
   GTGTGCCGCATCCGGTACGCGGCACCGTTTCGTTGGTCGAGTGGCACATGGCCCGCGCACGTAATCATATTCATGTTCTGGAAGAGAACACGGC                                                                                           <  SRR4302537.1484365/94‑1 (MQ=60)
   GTGTGCCGCATCCGGTACGCGGCACCGTTTCGTTGGTCGAGTGGCACATGGCCCGCGCACGTAATCATATTCATGTTCTGGAAGAGAACACGGC                                                                                           >  SRR4302537.5448459/1‑94 (MQ=60)
      TGCCGCATCCGGTACGCGGCACCGTTTCGTTGGTCGAGTGGCACATGGCCCGCGCACGTAATCATATTCATGTTCTGGAAGAGAACACGGCGCT                                                                                        <  SRR4302537.4376297/94‑1 (MQ=60)
      TGCCGCATCCGGTACGCGGCACCGTTTCGTTGGTCGAGTGGCACATGGCCCGCGCACGTAATCATATTCATGTTCTGGAAGAGAACACGGCGCT                                                                                        >  SRR4302537.4158854/1‑94 (MQ=60)
       GCCGCATCCGGTACGCGGCACCGTTTCGTTGGTCGAGTGGCACATGGCCCGCGCACGTAATCATATTCATGTTCTGGAAGAGAACACGGCGCTG                                                                                       <  SRR4302537.5946794/94‑1 (MQ=60)
       GCCGCATCCGGTACGCGGCACCGTTTCGTTGGTCGAGTGGCACATGGCCCGCGCACGTAATCATATTCATGTTCTGGAAGAGAACACGGCGCTG                                                                                       <  SRR4302537.986432/94‑1 (MQ=60)
        CCGCATCCGGTACGCGGCACCGTTTCGTTGGTCGAGTGGCACATGGCCCGCGCACGTAATCATATTCATGTTCTGGAAGAGAACACGGCGCTGT                                                                                      >  SRR4302537.6124876/1‑94 (MQ=60)
         CGCATCCGGTACGCGGCCCCGTTTCGTTGGTCGAGTGGCACATGGCCCGCGCACGTAATCATATTCATGTTCTGGAAGAGAACACGGCGCTGTT                                                                                     <  SRR4302537.423548/94‑1 (MQ=60)
         CGCATCCGGTACGCGGCACCGTTTCGTTGGTCGAGTGGCACATGGCCCGCGCACGTAATCATATTCATGTTCTGGAAGAGAACACGGCGCTGTT                                                                                     >  SRR4302537.6292004/1‑94 (MQ=60)
         CGCATCCGGTACGCGGCACCGTTTCGTTGGTCGAGTGGCACATGGCCCGCGCACGTAATCATATTCATGTTCTGGAAGAGAACACGGCGCTGTT                                                                                     >  SRR4302537.70744/1‑94 (MQ=60)
           CATCCGGTACGCGGCACCGTTTCGTTGGTCGAGTGGCACATGGCCCGCGCACGTAATCATATTCATGTTCCGGAAGAGAACACGGCGCTGTTGA                                                                                   >  SRR4302537.3747005/1‑94 (MQ=60)
            ATCCGGTACGCGGCACCGTTTCGTTGGTCGAGTGGCACATGGCCCGCGCACGTAATCATATTCATGTTCTGGAAGAGAACACGGCGCTGTTGAT                                                                                  <  SRR4302537.338178/94‑1 (MQ=60)
            ATCCGGTACGCGGCACCGTTTCGTTGGTCGAGTGGCACATGGCCCGCGCACGTAATCATATTCATGTTCTGGAAGAGAACACGGCGCTGTTGAT                                                                                  <  SRR4302537.212334/94‑1 (MQ=60)
            ATCCGGTACGCGGCACCGTTTCGTTGGTCGAGTGGCACATGGCCCGCGCACGTAATCATATTCATGTTCTGGAAGAGAACACGGCGCTGTTGAT                                                                                  >  SRR4302537.2098727/1‑94 (MQ=60)
            ATCCGGTACGCGGCACCGTTTCGTTGGTCGAGTGGCACATGGCCCGCGCACGTAATCATATTCATGTTCTGGAAGAGAACACGGCGCTGTTGAT                                                                                  <  SRR4302537.4574998/94‑1 (MQ=60)
            ATCCGGTACGCGGCACCGTTTCGTTGGTCGAGTGGCACATGGCCCGCGCACGTAATCATATTCATGTTCTGGAAGAGAACACGGCGCTGTTGAT                                                                                  >  SRR4302537.4181086/1‑94 (MQ=60)
             TCCGGTACGCGGCACCGTTTCGTTGGTCGAGTGGCACATGGCCCGCGCACGTAATCATATTCATGTTCTGGAAGAGAACACGGCGCTGTTGATG                                                                                 >  SRR4302537.2687102/1‑94 (MQ=60)
              CCGGTACGCGGCACCGTTTCGTTGGTCGAGTGGCACATGGCCCGCGCACGTAATCATATTCATGTTCTGGAAGAGAACACGGCGCTGTTGATGG                                                                                <  SRR4302537.957492/94‑1 (MQ=60)
                GGTACGCGGCACCGTTTCGTTGGTCGAGTGGCACATGGCCCGTGCACGTAATAATATTCATGTTCTGGAAGAGAACACAGCGCTGTTGATGGGA                                                                              >  SRR4302537.1137232/1‑94 (MQ=60)
                 GTACGCGGCACCGTTTCGTTGGTCGAGTGGCACATGGCCCGCGCACGTAATCATATTCATGTTCTGGAAGAGAACACGGCGCTGTTGATGGAAC                                                                             >  SRR4302537.1047684/1‑94 (MQ=60)
                   ACGCGGCACCGTTTCGTTGGTCGAGTGGCACATGGCCCGCGCACGTAATCATATTCATGTTCTGGAAGAGAACACGGCGCTGTTGATGGAACAG                                                                           <  SRR4302537.2629310/94‑1 (MQ=60)
                    TGCGGCACCGTTTCGTTGGTCGAGTGGCACATGGCCCGCGCACGTAATCATATTCATGTTCTGGAAGAGAACACGGCGCTGTTGATGGAACAGG                                                                          <  SRR4302537.3709538/94‑1 (MQ=60)
                    CGCGGCACCGTTTCGTTGGTCGAGTGGCACATGGCCCGCGCACGTAATCATATTCATGTTCTGGAAGAGAACACGGCGCTGTTGATGGAACAGG                                                                          >  SRR4302537.3908499/1‑94 (MQ=60)
                      CGGCACCGTTTCGTTGGTCGAGTGGCACATGGCCCGCGCACGTAATCATATTCATGTTCTGGAAGAGAACACGGCGCTGTTGATGGAACAGGCT                                                                        <  SRR4302537.732001/94‑1 (MQ=60)
                       GGCACCGTTTCGTTGGTCCAGTGGCACATGGCCCGCGCACGTAATCATATTCATGTTCTGGAAGAGAACACGGCGCTGTTGATGGAACAGGCTA                                                                       >  SRR4302537.2666755/1‑94 (MQ=60)
                         gactGTTTCGTTGGTCGAGTGGCACAGGGCCCGCGCACGTAATCATATTCATGTTCGGGAAGAGAACACGGCGCTGTTGATGGAACAGGCTATC                                                                     <  SRR4302537.6231912/90‑1 (MQ=60)
                         CACCGTTTCGTTGGTCGAGTGGCACATGGCCCGCGCACGTAATCATATTCATGTTCTGGAAGAGAACACGGCGCTGTTTATGGAACAGGCTATC                                                                     >  SRR4302537.4343294/1‑94 (MQ=60)
                         CACCGTTTCGTTGGTCGAGTGGCACATGGCCCGCGCACGTAATCATATTCATGTTCTGGAAGAGAACACGGCGCTGTTGATGGAACAGGCTATC                                                                     <  SRR4302537.5719134/94‑1 (MQ=60)
                         CACCGTTTCGTTGGTCGAGTGGCACATGGCCCGCGCACGTAATCATATTCATGTTCTGGAAGAGAACACGGCGCTGTTGATGGAACAGGATATC                                                                     >  SRR4302537.152412/1‑94 (MQ=60)
                          ACCGTTTCGTTGGTCGAGTGGCACATGGCCCGCGCACGTAATCATATTCATGTTCTGGAAGAGAACACGGCGCTGTTGATGGAACAGGCTATCG                                                                    >  SRR4302537.147780/1‑94 (MQ=60)
                          ACCGTTTCGTTGGTCGAGTGGCACATGGCCCGCGCACGTAATCATATTCATGTTCTGGAAGAGAACACGGCGCTGTTGATGGAACAGGCTATCG                                                                    >  SRR4302537.774153/1‑94 (MQ=60)
                            CGTTTCGTTGGTCGAGTGGCACATGGCCCGCGCACGTAATCATATTCATGTTCTGGAAGAGAACACGGCGCTGTTGATGGAACAGGCTATCGCC                                                                  >  SRR4302537.2838480/1‑94 (MQ=60)
                              TTTCGTTGGTCGAGTGGCACATGGCCCGCGCACGTAATCATATTCATGTTCTGGAAGAGAACACGGCGCTGTTGATGGAACAGGCTATCGCCAA                                                                <  SRR4302537.1540170/94‑1 (MQ=60)
                               TTCGTTGGTCGAGTGGCACATGGCCCGCGCACGTAATCATATTCATGTTCTGGAAGAGAACACGGCGCTGTTGATGGAACAGGCTATCGCCAAC                                                               >  SRR4302537.2004124/1‑94 (MQ=60)
                               TTCGTTGGTCGAGTGGCACATGGCCCGCGCACGTAATCATATTCATGTTCTGGAAGAGAACACGGCGCTGTTGATGGAACAGGCTATCGCCAAC                                                               <  SRR4302537.2162218/94‑1 (MQ=60)
                                 CGTTGGTCGAGTGGCACATGGCCCGCGCACGTAATCATATTCATGTTCTGGAAGAGAACACGGCGCTGTTGATGGAACAGGCTATCGCCAACGA                                                             >  SRR4302537.5459868/1‑94 (MQ=60)
                                     GGTCGAGTGGCACATGGCCCGCGCACGTAATCATATTCATGTTCTGGAAGAGAACACGGCGCTGTTGATGGAACAGGCTATCGACAACGAAGGC                                                         >  SRR4302537.4187971/1‑94 (MQ=60)
                                      GTCGAGTGGCACATGGCCCGCGCACGTAATCATATTCATGTTCTGGAAGAGAACACGGCGCTGTTGATGGAACAGGCTATCGCCAACGAAGGCC                                                        <  SRR4302537.2288198/94‑1 (MQ=60)
                                        CGAGTGGCACATGGCCCGCGCACGTAATCATATTCATGTTCTGGAAGAGAACACGGCGCTGTTGATGGAACAGGCTATCGCCAACGAAGGCCTG                                                      >  SRR4302537.111301/1‑94 (MQ=60)
                                        CGAGTGGCACATGGCCCGCGCACGTAATCATATTCATGTTCTGGAAGAGAACACGGCGCTGTTGATGGAACAGGCTATCGCCAACGAAGGCCTG                                                      <  SRR4302537.1933935/94‑1 (MQ=60)
                                         GAGTGGCACATGGCCCGCGCACGTAATCATATTCATGTTCTGGAAGAGAACACGGCGCTGTTGATGGAACAGGCTATCGCCAACGAAGGCCTGT                                                     >  SRR4302537.6495140/1‑94 (MQ=60)
                                          AGTGGCACATGGCCCGCGCACGTAATCATATTCATGTTCTGGAAGAGAACACGGCGCTGTTGATGGAACAGGCTATCGCCAACGAAGGCCTGTT                                                    >  SRR4302537.1836048/1‑94 (MQ=60)
                                           GTGGCACATGGCCCGCGCACGTAATCATATTCATGTTCTGGAAGAGAACACGGCGCTGTTGATGGAACAGGCTATCGCCAACGAAGGCCTGTTT                                                   <  SRR4302537.5942419/94‑1 (MQ=60)
                                           GTGGCACATGGCCCGCGCACGTAATCATATTCATGTTCTGGAAGAGAACACGGCGCTGTTGATGGAACAGGCTATCGCCAACGAAGGCCTGTTT                                                   >  SRR4302537.4061079/1‑94 (MQ=60)
                                            TGGCACATGGCCCGCGCACGTAATCTTATTCATGTTCTGGAAGAGAACACGGCGCTGATGATGGAACAGGCTATCGCCAACGAAGTCCTGTTTT                                                  >  SRR4302537.1345484/1‑94 (MQ=60)
                                            TGGCACATGGCCCGCGCACGTAATCATATTCATGTTCTGGAAGAGAACACGGCGCTGTTGATGGAACAGGCTATCGCCAACGAAGGCCTGTTTT                                                  >  SRR4302537.4120187/1‑94 (MQ=60)
                                              GCACATGGCCCGCGCACGTAATCATATTCATGTTCTGGAAGAGAACACGGCGCTGTTGATGGAACAGGCTATCGCCAACGAAGGCCTGTTTTAT                                                >  SRR4302537.980327/1‑94 (MQ=60)
                                               CACATGGCCCGCGCACGTAATCATATTCATGTTCTGGAAGAGAACACGGCGCTGTTGATGGAACAGGCTATCGCCAACGAAGGCCTGTTTTATC                                               >  SRR4302537.1126310/1‑94 (MQ=60)
                                               CACATGGCCCGCGCACGTAATCATATTCATGTTCTGGAAGAGAACACGGCGCTGTTGATGGAACAGGCTATCGCCAACGAAGGCCTGTTTTATC                                               >  SRR4302537.5785103/1‑94 (MQ=60)
                                               CACATGGCCCGCGCACGTAATCATATTCATGTTCTGGAAGAGAACACGGCGCTGTTGATGGAACAGGCTATCGCCAACGAAGGCCTGTTTTATC                                               >  SRR4302537.2300882/1‑94 (MQ=60)
                                               CACATGGCCCGCGCACGTAATCATATTCATGTTCTGGAAGAGAACACGGCGCTGTTGATGGAACAGGCTATCGCCAACGAAGGCCTGTTTTATC                                               <  SRR4302537.5609085/94‑1 (MQ=60)
                                               CACATGGCCCGCGCACGTAATCATATTCATGTTCTGGAAGAGAACACGGCGCTGTTGATGGAACAGGCTATCGCCAACGAAGGCCTGTTTTATC                                               <  SRR4302537.1651195/94‑1 (MQ=60)
                                               CACATGGCCCGCGCACGTAATCATATTCATGTTCTGGAAGAGAACACGGCGCTGTTGATGGAACAGGCTATCGCCAACGAAGGCCTGTTTTATC                                               <  SRR4302537.5936083/94‑1 (MQ=60)
                                                ACATGGCCCGCGCACGTAATCATATTCATGTTCTGGAAGAGAACACGGCGCTGTTGATGGAACAGGCTATCGCCAACGAAGGCCTGTTTTATCG                                              >  SRR4302537.734690/1‑94 (MQ=60)
                                                 CATGGCCCGCGCACGTAATCATATTCATGTTCTGGAAGAGAACACGGCGCTGTTGATGGAACAGGCTATCGCCAACGAAGGCCTGTTTTATCGC                                             >  SRR4302537.1602976/1‑94 (MQ=60)
                                                    GGCCCGCGCACGTAATCATATTCATGTTCTGGAAGAGAACACGGCGCTGTTGATGGAACAGGCTATCGCCAACGAAGGCCTGTTTTATCGCCTA                                          <  SRR4302537.4474270/94‑1 (MQ=60)
                                                     GCCCGCGCACGTAATCATATTCATGTTCTGGAAGAGAACACGGCGCTGTTGATGGAACAGGCTATCGCCAACGAAGGCCTGTTTTATCGCCTAC                                         >  SRR4302537.1417516/1‑94 (MQ=60)
                                                     GCCCGCGCACGTAATCATATTCATGTTCTGGAAGAGAACACGGCGCTGTTGATGGAACAGGCTATCGCCAACGAAGGCCTGTTTTATCGCCTAC                                         <  SRR4302537.72780/94‑1 (MQ=60)
                                                     GCCCGCGCACGTAATCATATTCATGTTCTGGAAGAGAACACGGCGCTGTTGATGGAACAGGCTATCGCCAACGAAGGCCTGTTTTATCGCCTAC                                         <  SRR4302537.1654770/94‑1 (MQ=60)
                                                       CCGCGCACGTAATCATATTCATGTTCTGGAAGAGAACACGGCGCTGTTGATGGAACAGGCTATCGCCAACGAAGGCCTGTTTTATCGCCTACTC                                       >  SRR4302537.1365099/1‑94 (MQ=60)
                                                       CCGCGCACGTAATCATATTCATGTTCTGGAAGAGAACACGGCGCTGTTGATGGAACAGGCTATCGCCAACGAAGGCCTGTTTTATCGCCTACTC                                       <  SRR4302537.1443297/94‑1 (MQ=60)
                                                        CGCGCACGTAATCATATTCATGTTCTGGAAGAGAACACGGCGCTGTTGATGGAACAGGCTATCGCCAACGAAGGCCTGTTTTATCGCCTACTCT                                      >  SRR4302537.4250471/1‑94 (MQ=60)
                                                          CGCACGTAATCATATTCATGTTCTGGAAGAGAACACGGCGCTGTTGATGGAACAGGCTATCGCCAACGAAGGCCTGTTTTATCGCCTACTCTAC                                    >  SRR4302537.5690384/1‑94 (MQ=60)
                                                          CGCACGTAATCATATTCATGTTCTGGAAGAGAACACGGCGCTGTTGATGGAACAGGCTATCGCCAACGAAGGCCTGTTTTATCGCCTACTCTAC                                    >  SRR4302537.5795438/1‑94 (MQ=60)
                                                          CGCACGTAATCATATTCATGTTCTGGAAGAGAACACGGCGCTGTTGATGGAACAGGCTATCGCCAACGAAGGCCTGTTTTATCGCCTACTCTAC                                    >  SRR4302537.1814059/1‑94 (MQ=60)
                                                           GCACGTAATCATATTCATGTTCTGGAAGAGAACACGGCGCTGTTGATGGAACAGGCTATCGCCAACGAAGGCCTGTTTTATCGCCTACTCTACC                                   >  SRR4302537.6061797/1‑94 (MQ=60)
                                                           GCACGTAATCATATTCATGTTCTGGAAGAGAACACGGCGCTGTTGATGGAACAGGCTATCGCCAACGAAGGCCTGTTTTATCGCCTACTCTACC                                   <  SRR4302537.2950004/94‑1 (MQ=60)
                                                             ACGTAATCATATTCATGTTCTGGAAGAGAACACGGCGCTGTTGATGGAACAGGCTATCGCCAACGAAGGCCTGTTTTATCGCCTACTCTACCTG                                 >  SRR4302537.2148135/1‑94 (MQ=60)
                                                              CGTAATCATATTCATGTTCTGGAAGAGAACACGGCGCTGTTGATGGAACAGGCTATCGCCAAGGAAGGCCTGTTTTATCGCCTAGTCTACCTGC                                >  SRR4302537.5843241/1‑94 (MQ=60)
                                                              CGTAATCATATTCATGTTCTGGAAGAGAACACGGCGCTGTTGATGGAACAGGCTATCGCCAACGAAGGCCTGTTTTATCGCCTACTCTACCTGC                                >  SRR4302537.6092232/1‑94 (MQ=60)
                                                              CGTAATCATATTCATGTTCTGGAAGAGAACACGGCGCTGTTGATGGAACAGGCTATCGCCAACGAAGGCCTGTTTTATCGCCTACTCTACCTGC                                >  SRR4302537.5388336/1‑94 (MQ=60)
                                                               GTAATCATATTCATGTTCTGGAAGAGAACACGGCGCTGTTGATGGAACAGGCTATCGCCAACGAAGGCCTGTTTTATCGTCTACTCTACCTGCA                               >  SRR4302537.1502922/1‑94 (MQ=60)
                                                               GTAATCATATTCATGTTCTGGAAGAGAACACGGCGCTGTTGATGGAACAGGCTATCGCCAACGAAGGCCTGTTTTATCGCCTACTCTACCTGCA                               <  SRR4302537.4944571/94‑1 (MQ=60)
                                                                TAATCATATTCATGTTCTGGAAGAGAACACGGCGCTGTTGATGGAACAGGCTATCGCCAACGAAGGCCTGTTTTATCGCCTACTCTACCTGCAG                              <  SRR4302537.1456398/94‑1 (MQ=60)
                                                                TAATCATATTCATGTTCTGGAAGAGAACACGGCGCTGTTGATGGAACAGGCTATCGCCAACGAAGGCCTGTTTTATCGCCTACTCTACCTGCAG                              >  SRR4302537.1098748/1‑94 (MQ=60)
                                                                TAATCATATTCATGTTCTGGAAGAGAACACGGCGCTGTTGATGGAACAGGCTATCGCCAACGAAGGCCTGTTTTATCGCCTACTCTACCTGCAG                              >  SRR4302537.6035751/1‑94 (MQ=60)
                                                                 AATCATATTCATGTTCTGGAAGAGAACACGGCGCTGTTGATGGAACAGGCTATCGCCAACGAAGGCCTGTTTTATCGCCTACTCTACCTGCAGC                             >  SRR4302537.2471824/1‑94 (MQ=60)
                                                                  ATCATATTCATGTTCTGGAAGAGAACACGGCGCTGTTGATGGAACAGGCTATCGCCAACGAAGGCCTGTTTTATCGCCTACTCTACCTGCAGCG                            <  SRR4302537.2918598/94‑1 (MQ=60)
                                                                  ATCATATTCATGTTCTGGAAGAGAACACGGCGCTGTTGATGGAAAAGGCTATCGCCAACGAAGGCCTGTTTTATCGCCTACTCTACCTGCAGCC                            >  SRR4302537.645277/1‑94 (MQ=60)
                                                                   TCATATTCATGTTCTGGAAGAGAACACGGCGCTGTTGATGGAACAGGCTATCGCCAACGAAGGCCTGTTTTATCGCCTACTCTACCTGCAGCGC                           >  SRR4302537.6105463/1‑94 (MQ=60)
                                                                     ATATTCATGTTCTGGAAGAGAACACGGCGCTGTTGATGGAACAGGCTATCGCCAACGAAGGCCTGTTTTATCGCCTACTCTACCTGCAGCGCAG                         >  SRR4302537.5690087/1‑94 (MQ=60)
                                                                     ATATTCATGTTCTGGAAGAGAACACGGCGCTGTTGATGGAACAGGCTATCGCCAACGAAGGCCTGTTTTATCGCCTACTCTACCTGCAGCGCAG                         >  SRR4302537.4379241/1‑94 (MQ=60)
                                                                     ATATTCATGTTCTGGAAGAGAACACGGCGCTGTTGATGGAACAGGCTATCGCCAACGAAGGCCTGTTTTATCGCCTACTCTACCTGCAGCGCAG                         >  SRR4302537.1365068/1‑94 (MQ=60)
                                                                      TATTCATGTTCTGGAAGAGAACACGGCGCTGTTGATGGAACAGGCTATCGCCAACGAAGGCCTGTTTTATCGCCTACTCTACCTGCAGCGCAGT                        <  SRR4302537.1169446/94‑1 (MQ=60)
                                                                      TATTCATGTTCTGGAAGAGAACACGGCGCTGTTGATGGAACAGGCTATCGCCAACGAAGGCCTGTTTTATCGCCTACTCTACCTGCAGCGCAGT                        >  SRR4302537.4703457/1‑94 (MQ=60)
                                                                      TATTCATGTTCTGGAAGAGAACACGGCGCTGTTGATGGAACAGGCTATCGCCAACGAAGGCCTGTTTTATCGCCTACTCTACCTGCAGCGCAGT                        <  SRR4302537.1418626/94‑1 (MQ=60)
                                                                       ATTCATGTTCTGGAAGAGAACACGGCGCTGTTGATGGAACAGGCTATCGCCAACGAAGGCCTGTTTTATCGCCTACTCTACCTGCAGCGCAGTC                       <  SRR4302537.3087431/94‑1 (MQ=60)
                                                                        TTCATGTTCTGGAAGAGAACACGGCGCTGTTGATGGAACAGGCTATCGCCAACGAAGGCCTGTTTTATCGCCTACTCTACCTGCAGCGCAGTCT                      <  SRR4302537.3821719/94‑1 (MQ=60)
                                                                        TTCATGTTCTGGAAGAGAACACGGCGCTGTTGATGGAACAGGCTATCGCCAACGAAGGCCTGTTTTATCGCCTACTCTACCTGCAGCGCAGTCC                      >  SRR4302537.4193538/1‑94 (MQ=60)
                                                                        TTCATGTTCTGGAAGAGAACACGGCGCTGTGGATGGAACAGGCTATCGCCAACGAAGGCCTGTTGTATCGCCTACTCTACCTGCAGCGCAGTCT                      <  SRR4302537.3328684/94‑1 (MQ=60)
                                                                         TCATGTTCTGGAAGAGAACACGGCGCTGTTGATGGAACAGGCTATCGCCAACGAAGGCCTGTTTTATCGCCTACTCTACCTGCAGCGCAGTCTC                     <  SRR4302537.5290085/94‑1 (MQ=60)
                                                                          CATGTTCTGGAAGAGAACACGGCGCTGTTGATGGAACAGGCTATCGCCAACGAAGGCCTGTTTTATCGCCTACTCTACCTGCAGCGCAGTCTCA                    >  SRR4302537.3490382/1‑94 (MQ=60)
                                                                          CATGTTCTGGAAGAGAACACGGCGCTGTTGATGGAACAGGCTATCGCCAACGAAGGCCTGTTTTATCGCCTACTCTACCTGCAGCGCAGTCTCA                    >  SRR4302537.2558884/1‑94 (MQ=60)
                                                                          CATGTTCTGGAAGAGAACACGGCGCTGTTGATGGAACAGGCTATCGCCAACGAAGGCCTGTTTTATCGCCTACTCTACCTGCAGCGCAGTCTCA                    >  SRR4302537.1341129/1‑94 (MQ=60)
                                                                            TGTTCTGGAAGAGAACACGGCGCTGTTGATGGAACAGGCTATCGCCAACGAAGGCCTGTTTTATCGCCTACTCTACCTGCAGCGCAGTCTCACC                  >  SRR4302537.257825/1‑94 (MQ=60)
                                                                             GTTCTGGAAGAGAACACGGCGCTGTTGATGGAACAGGCTATCGCCAACGAAGGCCTGTTTTATCGCCTACTCTACCTGCAGCGCAGTCTCACCC                 >  SRR4302537.6400428/1‑94 (MQ=60)
                                                                                CTGGAAGAGAACACGGCGCTGTTGATGGAACAGGCTATCGCCAACGAAGGCCTGTTTTATCGCCTACTCTACCTGCAGCGCAGTCTCACCGCCG              >  SRR4302537.6089375/1‑94 (MQ=60)
                                                                                 TGGAAGAGAACACGGCGCTGTTGATGGAACAGGCTATCGCCAACGAAGGCCTGTTTTATTGCCTACTCTACCTGCAGCGCAGTCTCACCGCCGC             >  SRR4302537.4384870/1‑94 (MQ=60)
                                                                                  GGAAGAGAACACGGCGCTGTTGATGGAACAGGCTATCGCCAACGAAGGCCTGTTTTATCGCCTACTCTACCTGCAGCGCAGTCTCACCGCCGCC            >  SRR4302537.3735946/1‑94 (MQ=60)
                                                                                    AAGAGAACACGGCGCTGTTGATGGAACAGGCTATCGCCAACGAAGGCCTGTTTTATCGCCTACTCTACCTGCAGCGCAGTCTCACCGCCGCCAG          >  SRR4302537.6096638/1‑94 (MQ=60)
                                                                                    AAGAGAACACGGCGCTGTTGATGGAACAGGCTATCGCCAACGAAGGCCTGTTTTATCGCCTACTCTACCTGCAGCGCAGTCTCACCGCCGCCAG          >  SRR4302537.1297390/1‑94 (MQ=60)
                                                                                    AAGAGAACACGGCGCTGTTGATGGAACAGGCTATCGCCAACGAAGGCCTGTTTTATCGCCTACTCTACCTGCAGCGCAGTCTCACCGCCGCCAG          <  SRR4302537.655349/94‑1 (MQ=60)
                                                                                       AGAACACGGCGCTGTTGATGGAACAGGCTATCGCCAACGAAGGCCTGTTTTATCGCCTACTCTACCTGCAGCGCAGTCTCACCGCCGCCAGCAG       >  SRR4302537.2607965/1‑94 (MQ=60)
                                                                                       AGAACACGGCGCTGTTGATGGAACAGGCTATCGCCAACGAAGGCCTGTTTTATCGCCTACTCTACCTGCAGCGCAGTCTCACCGCCGCCAGCAG       >  SRR4302537.4418515/1‑94 (MQ=60)
                                                                                        GAACACGGCGCTGTTGATGGAACAGGCTATCGCCAACGAAGGCCTGTTTTATCGCCTACTCTACCTGCAGCGCAGTCTCACCGCCGCCAGCAGT      <  SRR4302537.1999346/94‑1 (MQ=60)
                                                                                          ACACGGCGCTGTTGATGGAACAGGCTATCGCCAACGAAGGCCTGTTTTATCGCCTACTCTACCTGCAGCGCAGTCTCACCGCCGCCAGCAGTCT    <  SRR4302537.6427735/94‑1 (MQ=60)
                                                                                           CACGGCGCTGTTGATGGAACAGGCTATCGCCAACGAAGGCCTGTTTTATCGCCTACTCTACCTGCAGCGCAGTCTCACCGCCGCCAGCAGTCTC   >  SRR4302537.2603235/1‑94 (MQ=60)
                                                                                           CACGGCGCTGTTGATGGAACAGGCTATCGCCAACGAAGGCCTGTTTTATCGCCTACTCTACCTGCAGCGCAGTCTCACCGCCGCCAGCAGTCTC   <  SRR4302537.1040774/94‑1 (MQ=60)
                                                                                           CACGGCGCTGTTGATGGAACAGGCTATCGCCAACGAAGGCCTGTTTTATCGCCTACTCTACCTGCAGCGCAGTCTCACCGCCGCCAGCAGTCTC   <  SRR4302537.2408473/94‑1 (MQ=60)
                                                                                            ACGGCGCTGTTGATGGAACAGGCTATCGCCAACGAAGGCCTGTTTTATCGCCTACTCTACCTGCAGCGCAGTCTCACCGCCGCCAGCAGTCTCG  <  SRR4302537.409989/94‑1 (MQ=60)
                                                                                             |                                                                                            
TACGTGTGCCGCATCCGGTACGCGGCACCGTTTCGTTGGTCGAGTGGCACATGGCCCGCGCACGTAATCATATTCATGTTCTGGAAGAGAACATGGCGCTGTTGATGGAACAGGCTATCGCCAACGAAGGCCTGTTTTATCGCCTACTCTACCTGCAGCGCAGTCTCACCGCCGCCAGCAGTCTCG  >  NC_000913/3993317‑3993502

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 2 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑