Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I2 R1 882 254.4 12957588 98.0% 12698436 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 4,004,979 +G coding (1511/1833 nt) recQ → ATP‑dependent DNA helicase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,004,9791.G100.0% 603.1 / NA 173L504R (CTC→CGC) recQATP‑dependent DNA helicase
Reads supporting (aligned to +/- strand):  ref base . (0/0);  new base G (84/89);  total (84/89)

Only 100 of 194 total aligned reads displayed.

GTGATCCGCCAGCTGATTCACCTCGGCCTGGTGACGCAAAATATTGCCCAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGCT‑CGCG‑‑G‑‑AATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACCGAAAGCGATGCAGAAATCGTTCGGCGGCAA  >  NC_000913/4004888‑4005060
                                                                                            |                                                                                     
gTGATCCGCCAGCTGATTCACCTCGGCCTGGTGACGCAAAATATTGCCCAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGCtg                                                                                       >  1:429294/1‑92 (MQ=255)
 tGATCCGCCAGCTGATTCACCTCGGCCTGGTGACGCAAAATATTGCCCAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGCtgc                                                                                      >  2:1188114/1‑91 (MQ=255)
 tGATCCGCCAGCTGATTCACCTCGGCCTGGTGACGCAAAATATTGCCCAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGCtgc                                                                                      >  1:2602253/1‑91 (MQ=255)
 tGATCCGCCAGCTGATTCACCTCGGCCTGGTGACGCAAAATATTGCCCAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGCtgc                                                                                      <  2:6392972/93‑3 (MQ=255)
  gATCCGCCAGCTGATTCACCTCGGCCTGGTGACGCAAAATATTGCCCAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGCtgcg                                                                                     <  2:4074352/93‑4 (MQ=255)
   aTCCGCCAGCTGATTCACCTCGGCCTGGTGACGCAAAATATTGCCCAGCATTCTGCCCTACAACTGACAGAGGCCTCGCGCCCGGTGCtgcgc                                                                                    >  1:3475215/1‑89 (MQ=255)
   aTCCGCCAGCTGATTCACCTCGGCCTGGTGACGCAAAATATTGCCCAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGCtgcgc                                                                                    <  1:1021233/93‑5 (MQ=255)
   aTCCGCCAGCTGATTCACCTCGGCCTGGTGACGCAAAATATTGCCCAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGCtgcgc                                                                                    >  1:2093049/1‑89 (MQ=255)
   aTCCGCCAGCTGATTCACCTCGGCCTGGTGACGCAAAATATTGCCCAGCATTCTGACCTACAACTCACAGAGGCCGCGCGCCCGGTGCtgcgc                                                                                    >  2:5558553/1‑89 (MQ=255)
     ccGCCAGCTGATTCACCTCGGCCTGGTGACGCAAAATATTGCCCAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGCTGCGCg‑‑g                                                                                >  1:5108776/1‑93 (MQ=255)
        ccAGCTGATTCACCTCGGCCTGGTGACGCAAAATATTGCCCAGCATTCCGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGCTGCGCg‑‑gcga                                                                             >  1:332198/1‑90 (MQ=255)
         cAGCTGATTCACCTCGGCCTGGTGACGCAAAATATTGCCCAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGCTGCGCg‑‑gcgaa                                                                            <  2:3448122/93‑5 (MQ=255)
         cAGCTGATTCACCTCGGCCTGGTGACGCAAAATATTGCCCAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGCTGCGCg‑‑gcgaa                                                                            >  2:4446616/1‑89 (MQ=255)
               aTTCACCTCGGCCTGGTGACGCAAAATATTGCCCAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGCTGCGCGGCG‑‑AATCctct                                                                      <  1:6354429/93‑1 (MQ=255)
               aTTCACCTCGGCCTGGTGACGCAAAATATTGCCCAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGCTGCGCGGCG‑‑AATCctct                                                                      <  1:4568761/93‑1 (MQ=255)
                ttCACCTCGGCCTGGTGACGCAAAATATTGCCCAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGCTGCGCGGCG‑‑AATCCTCtt                                                                     >  1:5036569/1‑93 (MQ=255)
                 tCACCTCGGCCTGGTGACGCAAAATATTGCCCAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGCTGCGCGGCG‑‑AATCCTCttt                                                                    >  1:5244430/1‑93 (MQ=255)
                    ccTCGGCCTGGTGACGCAAAATATTGCCCAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGCTGCGCGGCG‑‑AATCCTCTTTGCa                                                                 >  2:4130613/1‑93 (MQ=255)
                    ccTCGGCCTGGTGACGCAAAATATTGCCCAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGCTGCGCG‑‑GCCAATCCTCTTTGcc                                                                 >  2:1656836/1‑92 (MQ=255)
                     cTCGGCCTGGTGACGCAAAATATTGCCCAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGCTGCGCGGCG‑‑AATCCTCTTTGCaa                                                                >  2:1481685/1‑93 (MQ=255)
                       cGGCCTGGTGACGCAAAATATTGCCCAGCATTCTGCCCTACAACGGACAGAGGCAGCGCGCCCGGTGCTGCGCGGAG‑‑AATCCTCTTTGCAACt                                                              <  2:3340055/93‑1 (MQ=255)
                        ggCCTGGTGACGCAAAATATTGCCCAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGCTGCGCGGCG‑‑AATCCTCTTTGCAACtt                                                             >  2:5661215/1‑93 (MQ=255)
                         gCCTGGTGACGCAAAATATTGCCCAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGCTGCGCGGCG‑‑AATCCTCTTTGCAACTtg                                                            >  1:2656962/1‑93 (MQ=255)
                         gCCTGGTGACGCAAAATATTGCCCAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGCTGCGCGGCG‑‑AATCCTCTTTGCAACTtg                                                            >  1:1799838/1‑93 (MQ=255)
                          ccTGGTGACGCAAAATATTGCCCAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGCTGCGCGGCG‑‑AATCCTCTTTGCAACTtgc                                                           <  2:227528/93‑1 (MQ=255)
                          ccTGGTGACGCAAAATATTGCCCAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGCTGCGCGGCG‑‑AATCCTCTTTGCAACTtgc                                                           <  2:5378944/93‑1 (MQ=255)
                           cTGGTGACGCAAAATATTTCCCAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGCTGCGCGGCG‑‑AATCCTCTTTGCAACTtgcc                                                          >  2:5989011/1‑93 (MQ=255)
                           cTGGTGACGCAAAATATTGCCCAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGCTGCGCGGCG‑‑AATCCTCTTTGCAACTtgcc                                                          <  2:5127300/93‑1 (MQ=255)
                           cTGGTGACGCAAAATATTGCCCAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGCTGCGCGGCG‑‑AATCCTCTTTGCAACTtgcc                                                          >  2:1576632/1‑93 (MQ=255)
                           cTGGTGACGCAAAATATTGCCCAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGCTGCGCGGCG‑‑AATCCTCTTTGCAACTtgcc                                                          <  2:3608916/93‑1 (MQ=255)
                           cTGGTGACGCAAAATATTGCCCAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGCTGCGCGGCG‑‑AATCCTCTTTGCAACTtgcc                                                          >  1:2154479/1‑93 (MQ=255)
                            tGGTGACGCAAAATATTGCCCAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGCTGCGCGGCG‑‑AATCCTCTTTGCAACTtgccg                                                         >  2:579915/1‑93 (MQ=255)
                             ggTGACGCAAAATATTGCCCAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGCTGCGCGGCG‑‑AATCCTCTTTGCAACTtgccgt                                                        <  1:1449683/93‑1 (MQ=255)
                              gTGACGCAAAATATTGCCCAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGCTGCGCGGCG‑‑AATCCTCTTTGCAACTtgccgtg                                                       >  2:3813945/1‑93 (MQ=255)
                               tGACGCAAAATATTGCCCAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGCTGCGCGGCG‑‑AATCCTCTTTGCAACTtgccgtgc                                                      >  1:1951159/1‑93 (MQ=255)
                                gACGCAAAATATTGCCCAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGCTGCGCGGCG‑‑AATCCTCTTTGCAACTtgccgtgcc                                                     >  2:1902452/1‑93 (MQ=255)
                                gACGCAAAATATTGCCCAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGCTGCGCGGCG‑‑AATCCTCTTTGCAACTtgccgtgcc                                                     >  1:5991656/1‑93 (MQ=255)
                                gACGCAAAATATTGCCCAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGCTGCGCGGCG‑‑AATCCTCTTTGCAACTtgccgtgcc                                                     <  1:3942437/93‑1 (MQ=255)
                                  cGCAAAATATTGCCCAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGCTGCGCGGCG‑‑AATCCTCTTTGCAACTTGCCGTGCcgc                                                   >  1:5805358/1‑93 (MQ=255)
                                     aaaaTATTGCCCAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGCTGCGCGGCG‑‑AATCCTCTTTGCAACTTGCCGTGCCGCGTa                                                >  1:3671530/1‑93 (MQ=255)
                                     aaaaTATTGCCCAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGCTGCGCGGCG‑‑AATCCTCTTTGCAACTTGCCGTGCCGCGTa                                                <  2:5292904/93‑1 (MQ=255)
                                       aaTATTGCCCAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGCTGCGCGGCG‑‑AATCCTCTTTGCAACTTGCCGTGCCGCGTATc                                              >  1:3179656/1‑93 (MQ=255)
                                       aaTATTGCCCAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGCTGCGCGGCG‑‑AATCCTCTTTGCAACTTGCCGTGCCGCGTATc                                              <  2:4774910/93‑1 (MQ=255)
                                       aaTATTGCCCAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGCTGCGCGGCG‑‑AATCCTCTTTGCAACTTGCCGTGCCGCGTATc                                              <  2:2760098/93‑1 (MQ=255)
                                       aaTATTGCCCAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGCTGCGCGGCG‑‑AATCCTCTTTGCAACTTGCCGTGCCGCGTATc                                              >  2:4562718/1‑93 (MQ=255)
                                       aaTATTGCCCAGCATTCTGCCCTACAACTGACAGAAGCCGCGCGCCCGGTGCTGCGCGGCG‑‑AATCCTCTTTGCAACTTGCCGTGCCGCGTATc                                              >  1:5621802/1‑93 (MQ=255)
                                        atatTGCCCAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGCTGCGCGGCG‑‑AATCCTCTTTGCAACTTGCCGTGCCGCGTATCg                                             >  1:5016551/1‑93 (MQ=255)
                                         tatTGCCCAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGCTGCGCGGCG‑‑AATCCTCTTTGCAACTTGCCGTGCCGCGTATCGt                                            <  1:5377665/93‑1 (MQ=255)
                                         tatTGCCCAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGCTGCGCGGCG‑‑AATCCTCTTTGCAACTTGCCGTGCCGCGTATCGt                                            <  2:3256374/93‑1 (MQ=255)
                                          atTGCCCAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGCTGCGCGGCG‑‑AATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTg                                           <  2:1326954/93‑1 (MQ=255)
                                          atTGCCCAGCATTCTGCCATACACCTGACAGAGGCCGCGCGCCCGGTGCTGCGCGGAG‑‑AATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTg                                           <  2:591240/93‑1 (MQ=255)
                                           ttGCCCAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGCTGCGCGGCG‑‑AATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTgg                                          >  2:5360018/1‑93 (MQ=255)
                                           ttGCCCAGCATTCTGCCCTACAACTGAAAGAGGCCGCGCGCCCGGTGCTGCGCGGCG‑‑AATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTgg                                          >  2:3210790/1‑93 (MQ=255)
                                            tGCCCAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGCTGCGCGGCG‑‑AATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGgc                                         <  1:958274/93‑1 (MQ=255)
                                            tGCCCAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGCTGCGCGGCG‑‑AATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGgc                                         <  2:2844088/93‑1 (MQ=255)
                                            tGCCCAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGCTGCGCGGCG‑‑AATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGgc                                         <  1:5726108/93‑1 (MQ=255)
                                             gCCCAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGCTGCGCGGCG‑‑AATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGgcg                                        >  2:2100591/1‑93 (MQ=255)
                                               ccAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGCTGCGCGGCG‑‑AATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCt                                      >  1:1172571/1‑93 (MQ=255)
                                                cAGCATTCTGCCCTACAACTGACAGACGCCTCGCGCCCGGTGCTGCGCGGCG‑‑AATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTc                                     >  1:4354123/1‑93 (MQ=255)
                                                  gCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGCTGCGCGGCG‑‑AATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCaa                                   >  1:4489989/1‑93 (MQ=255)
                                                  gCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGCTGCGCGGCG‑‑AATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCaa                                   >  2:4938372/1‑93 (MQ=255)
                                                     ttCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGCTGCGCGGCG‑‑AATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAAcc                                <  1:4815553/93‑1 (MQ=255)
                                                        tGCCCTACACCTGACAGAGGCCGCGCGCCCGGTGCTGCGCGGCG‑‑AATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACCGaa                             <  1:5585067/93‑1 (MQ=255)
                                                         gCCCTACAACTGACAGAGGCCGCGCGCCCGGTGCTGCGCGGCG‑‑AATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACCGaaa                            <  1:5159099/93‑1 (MQ=255)
                                                         gCCCTACAACTGACAGAGGCCGCGCGCCCGGTGCTGCGCGGCG‑‑AATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACCGaaa                            <  2:511110/93‑1 (MQ=255)
                                                          cccTACAACTGACAGAGGCCGCGCGCCCGGTGCTGCGCGGCG‑‑AATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACCGAAAg                           <  2:1096416/93‑1 (MQ=255)
                                                           ccTACATCTGACAGAGGCCGCCCGCGCGGTGCTGCGCGTCG‑‑AGTCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTAAAACCGAAAGc                          <  2:3091360/93‑1 (MQ=255)
                                                            cTACAACTGACAGAGGCCGCGCGCCCGGTGCTGCGCGGCG‑‑AATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACCGAAAGCg                         <  1:617940/93‑1 (MQ=255)
                                                             tACAACTGACAGAGGCCGCGCGCCCGGTGCTGCGCGGCG‑‑AATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACCGAAAGCGa                        >  2:3077873/1‑93 (MQ=255)
                                                              aCAACTGACAGAGGCCGCGCGCCCGGTGCTGCGCGGCG‑‑AATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACCGAAAGCGAt                       <  1:3688722/93‑1 (MQ=255)
                                                               cAACTGACAGAGGCCGCGCGCCCGGTGCTGCGCGGCG‑‑AATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACCGAAAGCGATg                      >  1:4441546/1‑93 (MQ=255)
                                                                aaCTGACAGAGGCCGCGCGCCCGGTGCTGCGCGGCG‑‑AATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACCGAAAGCGATGc                     >  1:789269/1‑93 (MQ=255)
                                                                 aCTGACAGAGGCCGCGCGCCCGGTGCTGCGCGGCG‑‑AATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACCGAAAGCGATGCa                    <  1:2507582/93‑1 (MQ=255)
                                                                 aCTGACAGAGGCCGCGCGCCCGGTGCTGCGCGGCG‑‑AATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACCGAAAGCGATGCa                    <  1:3151020/93‑1 (MQ=255)
                                                                 aCTGACAGAGGCCGCGCGCCCGGTGCTGCGCGGCG‑‑AATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACCGAAAGCGATGCa                    >  2:3927642/1‑93 (MQ=255)
                                                                  cTGACAGAGGCCGCGCGCCCGGTGCTGCGCGGCG‑‑AATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACCGAAAGCGATGCAg                   >  1:6064321/1‑93 (MQ=255)
                                                                    gACAGAGGCCGCGCGCCCGGTGCTGCGCGGCG‑‑AATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACCGAAAGCGATGCAGaa                 <  2:5617433/93‑1 (MQ=255)
                                                                      cAGAGGCCGCGCGCCCGGTGCTGCGCGGCG‑‑AATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACCGAAAGCGATGCAGAAAt               <  1:3370043/93‑1 (MQ=255)
                                                                       agagGCCGCGCGCCCGGTGCTGCGCGGCG‑‑AATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACCGAAAGCGATTCAGAAATc              >  2:6149055/1‑93 (MQ=255)
                                                                       agagGCCGCGCGCCCGGTGCTGCGCGGCG‑‑AATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACCGAAAGCGATGCAGAAAtg              >  1:3041344/1‑92 (MQ=255)
                                                                       agagGCCGCGCGCCCGGTGCTGCGCGGCG‑‑AATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACCGAAAGCGATGCAGAAATc              <  1:4209673/93‑1 (MQ=255)
                                                                       agagGCCGCGCGCCCGGTGCTGCGCGGCG‑‑AATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACCGAAAGCGATGCAGAAATc              >  2:1822926/1‑93 (MQ=255)
                                                                        gagGCCGCGCGCCCGGTGATGCGCGGCG‑‑AATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACCGAAAGCGATGCAGAAATCg             <  1:1149108/93‑1 (MQ=255)
                                                                         agGCCGCGCGCCCGGTGCTGCGCGGCG‑‑AATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACCGAAAGCGATGCAGAAATCGt            >  1:382361/1‑93 (MQ=255)
                                                                         agGCCGCGCGCCCGGTGCTGCGCGGCG‑‑AATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACCGAAAGCGATGCAGAAATCGt            <  2:1389214/93‑1 (MQ=255)
                                                                          ggCCGCGCGCCCGGTGCTGCGCGGCG‑‑AATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACCGAAAGCGATGCAGAAATCGtt           >  1:1607306/1‑93 (MQ=255)
                                                                          ggCCGCGCGCCCGGTGCTGCGCGGCG‑‑AATCCTCTTTGCAACTTGCCGTGCCACGTATCGTGGCGCTCAAACCGAAAGCGATGCATAAATCGtt           <  2:6277623/93‑1 (MQ=255)
                                                                           gCCGCGCGCCCGGTGCTGCGCGGCG‑‑AATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACCGAAAGCGATGCAGAAATCGTTc          <  2:311609/93‑1 (MQ=255)
                                                                           gCCGCGCGCCCGGTGCTGCGCGGCG‑‑AATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACCGAAAGCGATGCAGAAATCGTTc          <  1:6295159/93‑1 (MQ=255)
                                                                           gCCGCGCGCCCGGTGCTGCGCGGCG‑‑AATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACCGAAAGCGATGCAGAAATCGTTc          <  1:4899578/93‑1 (MQ=255)
                                                                            ccGCGCGCCCGGTGCTGCGCGGCG‑‑AATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACCGAAAGCGATGCAGAAATCGTTcg         <  1:1041639/93‑1 (MQ=255)
                                                                            ccGCGCGCCCGGTGCTGCGCGGCG‑‑AATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACCGAAAGCGATGCAGAAATCGTTcg         <  1:4821845/93‑1 (MQ=255)
                                                                            ccGCGCGCCCGGTGCTGCGCGGCG‑‑AATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACCGAAAGCGATGCAGAAATCGTTcg         <  1:3210074/93‑1 (MQ=255)
                                                                              gcgcgcCCGGTGCTGCGCGGCG‑‑AATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACCGAAAGCGATGCAGAAATCGTTcggc       >  2:3007197/1‑93 (MQ=255)
                                                                              gcgcgcCCGGTGCTGCGCGGCG‑‑AATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACCGAAAGCGATGCAGAAATCGTTcggc       <  2:2602512/93‑1 (MQ=255)
                                                                              gcgcgcCCGGTGCTGCGCGGCG‑‑AATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACCGAAAGCGATGCAGAAATCGTTcggc       <  1:2308635/93‑1 (MQ=255)
                                                                              gcgcgcCCGGTGCTGCGCGGCG‑‑AATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACCGAAAGCGATGCAGAAATCGTTcggc       <  2:800330/93‑1 (MQ=255)
                                                                               cgcgcCCGGTGCTGCGCGGCG‑‑AATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACCGAAAGCGATGCAGAAATCGTTcggcg      <  1:6018589/93‑1 (MQ=255)
                                                                               cgcgcCCGGTGCTGCGCGGCG‑‑AATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACCGAAAGCGATGCAGAAATCGTTcggcg      <  1:4681426/93‑1 (MQ=255)
                                                                                   cccGGTGCTGCGCGGCG‑‑AATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACCGAAAGCGATGCAGAAATCGTTCGGCGGCaa  >  2:5684734/1‑93 (MQ=255)
                                                                                            |                                                                                     
GTGATCCGCCAGCTGATTCACCTCGGCCTGGTGACGCAAAATATTGCCCAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGCT‑CGCG‑‑G‑‑AATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACCGAAAGCGATGCAGAAATCGTTCGGCGGCAA  >  NC_000913/4004888‑4005060

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
CGTGATCCGCCAGCTGATTCACCTCGGCCTGGTGACGCAAAATATTGCCCAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGCT‑CGCG‑‑GAATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACCGAAAGCGATGCAGAAATCGTTCGGCGGCAACTA  >  NC_000913/4004887‑4005063
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CGTGATCCGCCAGCTGATTCACCTCGGCCTGGTGACGCAAAATATTGCCCAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGCT‑G                                                                                       >  SRR4302537.432284/1‑94 (MQ=60)
 GTGATCCGCCAGCTGATTCACCTCGGCCTGGTGACGCAAAATATTGCCCAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGCTgc                                                                                       >  SRR4302537.2620448/1‑92 (MQ=60)
  TGATCCGCCAGCTGATTCACCTCGGCCTGGTGACGCAAAATATTGCCCAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGCT‑‑GCG                                                                                    <  SRR4302537.2483563/94‑1 (MQ=60)
   GATCCGCCAGCTGATTCACCTCGGCCTGGTGACGCAAAATATTGCCCAGCATTCTGCCCTACAACTGACAGAGGCCTCGCGCCCGGTGCTGCGC                                                                                     >  SRR4302537.3499807/1‑94 (MQ=60)
   GATCCGCCAGCTGATTCACCTCGGCCTGGTGACGCAAAATATTGCCCAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGCTGCGC                                                                                     >  SRR4302537.2199954/1‑94 (MQ=60)
   GATCCGCCAGCTGATTCACCTCGGCCTGGTGACGCAAAATATTGCCCAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGCTGCGC                                                                                     >  SRR4302537.2107809/1‑94 (MQ=60)
    ATCCGCCAGCTGATTCACCTCGGCCTGGTGACGCAAAATATTGCCCAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGCTGCGCG                                                                                    <  SRR4302537.1028270/94‑1 (MQ=60)
    ATCCGCCAGCTGATTCACCTCGGCCTGGTGACGCAAAATATTGCCCAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGCTGCGCG                                                                                    <  SRR4302537.4532529/94‑1 (MQ=60)
     TCCGCCAGCTGATTCACCTCGGCCTGGTGACGCAAAATATTGCCCAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGCTGCGCG‑‑G                                                                                 >  SRR4302537.5143978/1‑94 (MQ=60)
        GCCAGCTGATTCACCTCGGCCTGGTGACGCAAAATATTGCCCAGCATTCCGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGCTgcgcggcga                                                                                >  SRR4302537.334496/1‑85 (MQ=60)
          CAGCTGATTCACCTCGGCCTGGTGACGCAAAATATTGCCCAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGCTgcgcggcgaat                                                                              >  SRR4302537.2795711/1‑83 (MQ=60)
          CAGCTGATTCACCTCGGCCTGGTGACGCAAAATATTGCCCAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGCTgcgcggcgaat                                                                              >  SRR4302537.951259/1‑83 (MQ=60)
             CTGATTCACCTCGGCCTGGTGACGCAAAATATTGCCCAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGCTgcgcggcgactcct                                                                           >  SRR4302537.3385098/1‑80 (MQ=60)
             CTGATTCACCTCGGCCTGGTGACGCAAAATATTGCCCAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGCTGCGCGGCGAATCCT                                                                           >  SRR4302537.6020429/1‑94 (MQ=60)
              TGATTCACCTCGGCCTGGTGACGCAAAATATTGCCCAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGCTGCGCGGCGAATCCTC                                                                          >  SRR4302537.5454963/1‑94 (MQ=60)
              TGATTCACCTCGGCCTGGTGACGCAAAATATTGCCCAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGCTGCGCGGCGAATCCTC                                                                          >  SRR4302537.4565159/1‑94 (MQ=60)
              TGATTCACCTCGGCCTGGTGACGCAAAATATTGCCCAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGCTGCGCGGCGAATCCTC                                                                          >  SRR4302537.290656/1‑94 (MQ=60)
                ATTCACCTCGGCCTGGTGACGCAAAATATTGCCCAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGCTGCGCGGCGAATCCTCTT                                                                        <  SRR4302537.6397802/94‑1 (MQ=60)
                ATTCACCTCGGCCTGGTGACGCAAAATATTGCCCAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGCTGCGCGGCGAATCCTCTT                                                                        >  SRR4302537.5071241/1‑94 (MQ=60)
                ATTCACCTCGGCCTGGTGACGCAAAATATTGCCCAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGCTGCGCGGCGAATCCTCTT                                                                        <  SRR4302537.4600380/94‑1 (MQ=60)
                 TTCACCTCGGCCTGGTGACGCAAAATATTGCCCAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGCTGCGCGGCGAATCCTCTTT                                                                       >  SRR4302537.5058643/1‑94 (MQ=60)
                 TTCACCTCGGCCTGGTGACGCAAAATATTGCCCAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGCTGCGCGGCGAATCCTCTTT                                                                       >  SRR4302537.5280518/1‑94 (MQ=60)
                   CACCTCGGCCTGGTGACGCAAAATATTGCCCAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGCTGCGCGGCGAATCCTCTTTGC                                                                     <  SRR4302537.3371353/94‑1 (MQ=60)
                    ACCTCGGCCTGGTGACGCAAAATATTGCCCAGCATTCTGCCCTACAACTGACATAGGCCGCGCGCCCGGTGCTGCGCGGCGAATCCTCTTTGCA                                                                    >  SRR4302537.1949538/1‑94 (MQ=60)
                     CCTCGGCCTGGTGACGCAAAATATTGCCCAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGCTGCGCGGCGAATCCTCTTTGCAA                                                                   >  SRR4302537.6327897/1‑94 (MQ=60)
                      CTCGGCCTGGTGACGCAAAATATTGCCCAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGCTGCGCGGCGAATCCTCTTTGCAAC                                                                  >  SRR4302537.1970293/1‑94 (MQ=60)
                      CTCGGCCTGGTGACGCAAAATATTGCCCAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGCTGCGCGGCGAATCCTCTTTGCAAC                                                                  >  SRR4302537.4439638/1‑94 (MQ=60)
                       TCGGCCTGGTGACGCAAAATATTGCCCAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGCTGCGCGGCGAATCCTCTTTGCAACT                                                                 <  SRR4302537.2551567/94‑1 (MQ=60)
                        CGGCCTGGTGACGCAAAATATTGCCCAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGCTGCGCGGCGAATCCTCTTTGCAACTT                                                                <  SRR4302537.4134844/94‑1 (MQ=60)
                         GGCCTGGTGACGCAAAATATTGCCCAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGCTGCGCGGCGAATCCTCTTTGCAACTTG                                                               >  SRR4302537.2675532/1‑94 (MQ=60)
                         GGCCTGGTGACGCAAAATATTGCCCAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGCTGCGCGGCGAATCCTCTTTGCAACTTG                                                               >  SRR4302537.1812399/1‑94 (MQ=60)
                           CCTGGTGACGCAAAATATTGCCCAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGCTGCGCGGCGAATCCTCTTTGCAACTTGCC                                                             >  SRR4302537.2169664/1‑94 (MQ=60)
                           CCTGGTGACGCAAAATATTGCCCAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGCTGCGCGGCGAATCCTCTTTGCAACTTGCC                                                             <  SRR4302537.4239871/94‑1 (MQ=60)
                            CTGGTGACGCAAAATATTGCCCAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGCTGCGCGGCGAATCCTCTTTGCAACTTGCCG                                                            >  SRR4302537.4850697/1‑94 (MQ=60)
                              GGTGACGCAAAATATTGCCCAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGCTGCGCGGCGAATCCTCTTTGCAACTTGCCGTG                                                          <  SRR4302537.5179660/94‑1 (MQ=60)
                              GGTGACGCAAAATATTGCCCAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGCTGCGCGGCGAATCCTCTTTGCAACTTGCCGTG                                                          <  SRR4302537.1459783/94‑1 (MQ=60)
                               GTGACGCAAAATATTGCCCAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGCTGCGCGGCGAATCCTCTTTGCAACTTGCCGTGC                                                         >  SRR4302537.1964728/1‑94 (MQ=60)
                                TGACGCAAAATATTGCCCAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGCTGCGCGGCGAATCCTCTTTGCAACTTGCCGTGCC                                                        <  SRR4302537.5348483/94‑1 (MQ=60)
                                TGACGCAAAATATTGCCCAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGCTGCGCGGCGAATCCTCTTTGCAACTTGCCGTGCC                                                        >  SRR4302537.6032806/1‑94 (MQ=60)
                                 GACGCAAAATATTGCCCAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGCTGCGCGGCGAATCCTCTTTGCAACTTGCCGTGCCG                                                       >  SRR4302537.6085921/1‑94 (MQ=60)
                                 GACGCAAAATATTGCCCAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGCTGCGCGGCGAATCCTCTTTGCAACTTGCCGTGCCG                                                       <  SRR4302537.3970011/94‑1 (MQ=60)
                                  ACGCAAAATATTGCCCAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGCTGCGCGGCGAATCCTCTTTGCAACTTGCCGTGCCGC                                                      >  SRR4302537.5845164/1‑94 (MQ=60)
                                     CAAAATATTGCCCAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGCTGCGCGGCGAATCCTCTTTGCAACTTGCCGTGCCGCGTA                                                   >  SRR4302537.3697365/1‑94 (MQ=60)
                                      AAAATATTGCCCAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGCTGCGCGGCGAATCCTCTTTGCAACTTGCCGTGCCGCGTAT                                                  >  SRR4302537.1173742/1‑94 (MQ=60)
                                       AAATATTGCCCAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGCTGCGCGGCGAATCCTCTTTGCAACTTGCCGTGCCGCGTATC                                                 >  SRR4302537.993863/1‑94 (MQ=60)
                                       AAATATTGCCCAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGCTGCGCGGCGAATCCTCTTTGCAACTTGCCGTGCCGCGTATC                                                 >  SRR4302537.3202004/1‑94 (MQ=60)
                                       AAATATTGCCCAGCATTCTGCCCTACAACTGACAGAAGCCGCGCGCCCGGTGCTGCGCGGCGAATCCTCTTTGCAACTTGCCGTGCCGCGTATC                                                 >  SRR4302537.5660237/1‑94 (MQ=60)
                                        AATATTGCCCAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGCTGCGCGGCGAATCCTCTTTGCAACTTGCCGTGCCGCGTATCG                                                >  SRR4302537.5051111/1‑94 (MQ=60)
                                         ATATTGCCCAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGCTGCGCGGCGAATCCTCTTTGCAACTTGCCGTGCCGCGTATCGT                                               >  SRR4302537.4810026/1‑94 (MQ=60)
                                         ATATTGCCCAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGCTGCGCGGCGAATCCTCTTTGCAACTTGCCGTGCCGCGTATCGT                                               >  SRR4302537.2162842/1‑94 (MQ=60)
                                          TATTGCCCAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGCTGCGCGGCGAATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTG                                              <  SRR4302537.5414453/94‑1 (MQ=60)
                                            TTGCCCAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGCTGCGCGGCGAATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGC                                            >  SRR4302537.347770/1‑94 (MQ=60)
                                             TGCCCAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGCTGCGCGGCGAATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCG                                           <  SRR4302537.5115165/94‑1 (MQ=60)
                                             TGCCCAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGCTGCGCGGCGAATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCG                                           <  SRR4302537.5765306/94‑1 (MQ=60)
                                             TGCCCAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGCTGCGCGGCGAATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCG                                           <  SRR4302537.964884/94‑1 (MQ=60)
                                              GCCCAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGCTGCGCGGCGAATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGC                                          <  SRR4302537.3698190/94‑1 (MQ=60)
                                               CCCAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGCTGCGCGGCGAATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCT                                         >  SRR4302537.1180730/1‑94 (MQ=60)
                                                CCAGCATTCTGCCCTACAACTGACAGACGCCTCGCGCCCGGTGCTGCGCGGCGAATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTC                                        >  SRR4302537.4384273/1‑94 (MQ=60)
                                                 CAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGCTGCGCGGCGAATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCA                                       >  SRR4302537.5271360/1‑94 (MQ=60)
                                                 CAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGCTGCGCGGCGAATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCA                                       <  SRR4302537.6131960/94‑1 (MQ=60)
                                                  AGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGCTGCGCGGCGAATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAA                                      >  SRR4302537.4521047/1‑94 (MQ=60)
                                                  AGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGCTGCGCGGCGAATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAA                                      >  SRR4302537.5626770/1‑94 (MQ=60)
                                                     ATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGCTGCGCGGCGAATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACC                                   <  SRR4302537.948046/94‑1 (MQ=60)
                                                      TTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGCTGCGCGGCGAATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACCG                                  <  SRR4302537.4848792/94‑1 (MQ=60)
                                                       TCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGCTGCGCGGCGAATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTCGCGCTCAAACCGA                                 >  SRR4302537.1531323/1‑94 (MQ=60)
                                                        CTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGCTGCGCGGCGAATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACCGAA                                <  SRR4302537.6055479/94‑1 (MQ=60)
                                                         TGCCCTACACCTGACAGAGGCCGCGCGCCCGGTGCTGCGCGGCGAATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACCGAAA                               <  SRR4302537.5623253/94‑1 (MQ=60)
                                                          GCCCTACAACTGACAGAGGCCGCGCGCCCGGTGCTGCGCGGCGAATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACCGAAAG                              <  SRR4302537.5194657/94‑1 (MQ=60)
                                                            CCTACAACTGACAGAGGCCGCGCGCCCGGTGCTGCGCGGCGAATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACCGAAAGCG                            >  SRR4302537.5049383/1‑94 (MQ=60)
                                                            CCTACAACTGACAGAGGCCGCGCGCCCGGTGCTGCGCGGCGAATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACCGAAAGCG                            <  SRR4302537.5818381/94‑1 (MQ=60)
                                                             CTACAACTGACAGAGGCCGCGCGCCCGGTGCTGCGCGGCGAATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACCGAAAGCGA                           <  SRR4302537.622144/94‑1 (MQ=60)
                                                              TACAACTGACAGAGGCCGCGCGCCCGGTGCTGCGCGGCGAATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACCGAAAGCGAT                          <  SRR4302537.1132241/94‑1 (MQ=60)
                                                              TACAACTGACAGAGGCCGCGCGCCCGGTGCTGCGCGGCGAATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACCGAAAGCGAT                          <  SRR4302537.6366033/94‑1 (MQ=60)
                                                               ACAACTGACAGAGGCCGCGCGCCCGGTGCTGCGCGGCGAATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACCGAAAGCGATG                         <  SRR4302537.3714664/94‑1 (MQ=60)
                                                               ACAACTGACAGAGGCCGCGCGCCCGGTGCTGCGCGGCGAATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACCGAAAGCGATG                         <  SRR4302537.3300518/94‑1 (MQ=60)
                                                               ACAACTGACAGAGGCCGCGCGCCCGGTGCTGCGCGGCGAATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACCGAAAGCGATG                         >  SRR4302537.4472221/1‑94 (MQ=60)
                                                                CAACTGACAGAGGCCGCGCGCCCGGTGCTGCGCGGCGAATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACCGAAAGCGATGC                        >  SRR4302537.794689/1‑94 (MQ=60)
                                                                  ACTGACAGAGGCCGCGCGCCCGGTGCTGCGCGGCGAATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACCGAAAGCGATGCAG                      >  SRR4302537.6105987/1‑94 (MQ=60)
                                                                  ACTGACAGAGGCCGCGCGCCCGGTGCTGCGCGGCGAATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACCGAAAGCGATGCAG                      >  SRR4302537.1710577/1‑94 (MQ=60)
                                                                  ACTGACAGAGGCCGCGCGCCCGGTGCTGCGCGGCGAATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACCGAAAGCGATGCAG                      <  SRR4302537.3173116/94‑1 (MQ=60)
                                                                  ACTGACAGAGGCCGCGCGCCCGGTGCTGCGCGGCGAATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACCGAAAGCGATGCAG                      <  SRR4302537.2525223/94‑1 (MQ=60)
                                                                  ACTGACAGAGGCCGCGCGCCCGGTGCTGCGCGGCGAATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACCGAAAGCGATGCAG                      >  SRR4302537.5929973/1‑94 (MQ=60)
                                                                     GACAGAGGCCGCGCGCCCGGTGCTGCGCGGCGAATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACCGAAAGCGATGCAGAAA                   <  SRR4302537.2757678/94‑1 (MQ=60)
                                                                       CAGAGGCCGCGCGCCCGGTGCTGCGCGGCGAATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACCGAAAGCGATGCAGAAATG                 >  SRR4302537.3062537/1‑94 (MQ=60)
                                                                       CAGAGGCCGCGCGCCCGGTGCTGCGCGGCGAATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACCGAAAGCGATGCAGAAATC                 <  SRR4302537.3393923/94‑1 (MQ=60)
                                                                       CAGAGGCCGCGCGCCCGGTGCTGCGCGGCGAATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACCGAAAGCGATGCAGAAATC                 <  SRR4302537.4704227/94‑1 (MQ=60)
                                                                       CAGAGGCCGCGCGCCCGGTGCTGCGCGGCGAATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACCGAAAGCGATGCAGAAATC                 <  SRR4302537.4327825/94‑1 (MQ=60)
                                                                        AGAGGCCGCGCGCCCGGTGCTGCGCGGCGAATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACCGAAAGCGATGCAGAAATCG                <  SRR4302537.4239038/94‑1 (MQ=60)
                                                                         GAGGCCGCGCGCCCGGTGCTGCGCGGCGAATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACCGAAAGCGATGCAGAAATCGT               >  SRR4302537.385009/1‑94 (MQ=60)
                                                                         GAGGCCGCGCGCCCGGTGATGCGCGGCGAATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACCGAAAGCGATGCAGAAATCGT               <  SRR4302537.1157096/94‑1 (MQ=60)
                                                                          AGGCCGCGCGCCCGGTGCTGCGCGGCGAATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACCGAAAGCGATGCAGAAATCGTT              >  SRR4302537.3616889/1‑94 (MQ=60)
                                                                          AGGCCGCGCGCCCGGTGCTGCGCGGCGAATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACCGAAAGCGATGCAGAAATCGTT              >  SRR4302537.1618547/1‑94 (MQ=60)
                                                                          AGGCCGCGCGCCCGGTGCTGCGCGGCGAATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACCGAAAGCGATGCAGAAATCGTT              <  SRR4302537.390181/94‑1 (MQ=60)
                                                                           GGCCGCGCGCCCGGTGCTGCGCGGCGAATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACCGAAAGCGATGCAGAAATCGTTC             <  SRR4302537.3175192/94‑1 (MQ=60)
                                                                           GGCCGCGCGCCCGGTGCTGCGCGGCGAATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACCGAAAGCGATGCAGAAATCGTTC             <  SRR4302537.4512148/94‑1 (MQ=60)
                                                                            GCCGCGCGCCCGGTGCTGCGCGGCGAATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACCGAAAGCGATGCAGAAATCGTTCG            <  SRR4302537.6338285/94‑1 (MQ=60)
                                                                            GCCGCGCGCCCGGTGCTGCGCGGCGAATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACCGAAAGCGATGCAGAAATCGTTCG            <  SRR4302537.4933398/94‑1 (MQ=60)
                                                                             CCGCGCGCCCGGTGCTGCGCGGCGAATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACCGAAAGCGATGCAGAAATCGTTCGG           <  SRR4302537.1048832/94‑1 (MQ=60)
                                                                             CCGCGCGCCCGGTGCTGCGCGGCGAATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACCGAAAGCGATGCAGAAATCGTTCGG           <  SRR4302537.4855151/94‑1 (MQ=60)
                                                                             CCGCGCGCCCGGTGCTGCGCGGCGAATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACCGAAAGCGATGCAGAAATCGTTCGG           <  SRR4302537.3232646/94‑1 (MQ=60)
                                                                              CGCGCGCCCGGTGCTGCGCGGCGAATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCaagttgcaaagaggattcacggaatagatagg          >  SRR4302537.3224480/1‑62 (MQ=60)
                                                                              CGCGCGCCCGGTGCTGCGCGGCGAATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACCGAAAGCGATGCAGAAATCGTTCGGC          <  SRR4302537.3808831/94‑1 (MQ=60)
                                                                              CGCGCGCCCGGTGCTGCGCGGCGAATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACCGAAAGCGATGCAGAAATCGTTCGGC          <  SRR4302537.2634318/94‑1 (MQ=60)
                                                                               GCGCGCCCGGTGCTGCGCGGCGAATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACCGAAAGCGATGCAGAAATCGTTCGGCG         <  SRR4302537.2324932/94‑1 (MQ=60)
                                                                                CGCGCCCGGTGCTGCGCGGCGAATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACCGAAAGCGATGCAGAAATCGTTCGGCGG        <  SRR4302537.6059935/94‑1 (MQ=60)
                                                                                CGCGCCCGGTGCTGCGCGGCGAATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACCGAAAGCGATGCAGAAATCGTTCGGCGG        <  SRR4302537.4713811/94‑1 (MQ=60)
                                                                                 GCGCCCGGTGCTGCGCGGCGAATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACCGAAAGCGATGCAGAAATCGTTCGGCGGC       <  SRR4302537.5695154/94‑1 (MQ=60)
                                                                                 GCGCCCGGTGCTGCGCGGCGAATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACCGAAAGCGATGCAGAAATCGTTCGGCGGC       <  SRR4302537.1401501/94‑1 (MQ=60)
                                                                                   GCCCGGTGCTGCGCGGCGAATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACCGAAAGCGATGCAGAAATCGTTCGGCGGCAA     >  SRR4302537.3133016/1‑94 (MQ=60)
                                                                                    CCCGGTGCTGCGCGGCGAATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACCGAAAGCGATGCAGAAATCGTTCGGCGGCAAC    >  SRR4302537.5668450/1‑94 (MQ=60)
                                                                                    CCCGGTGCTGCGCGGCGAATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACCGAAAGCGATGCAGAAATCGTTCGGCGGCAAC    <  SRR4302537.5437167/94‑1 (MQ=60)
                                                                                     CCGGTGCTGCGCGGCGAATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACCGAAAGCGATGCAGAAATCGTTCGGCGGCAACT   <  SRR4302537.6135559/94‑1 (MQ=60)
                                                                                     CCGGTGCTGCGCGGCGAATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACCGAAAGCGATGCAGAAATCGTTCGGCGGCAACT   <  SRR4302537.5139087/94‑1 (MQ=60)
                                                                                     CCGGTGCTGCGCGGCGAATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACCGAAAGCGATGCAGAAATCGTTCGGCGGCAACT   <  SRR4302537.3233053/94‑1 (MQ=60)
                                                                                     CCGGTGCTGCGCGGCGAATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACCGAAAGCGATGCAGAAATCGTTCGGCGGCAACT   >  SRR4302537.2531509/1‑94 (MQ=60)
                                                                                     CCGGTGCTGCGCGGCGAATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACCGAAAGCGATGCAGAAAGCGTTCGGCGGCAACT   >  SRR4302537.877572/1‑94 (MQ=60)
                                                                                      CGGTGCTGCGCGGCGAATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACCGAAAGCGATGCAGAAATCGTTCGGCGGCAACTA  >  SRR4302537.5063155/1‑94 (MQ=60)
                                                                                            |                                                                                       
CGTGATCCGCCAGCTGATTCACCTCGGCCTGGTGACGCAAAATATTGCCCAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGCT‑CGCG‑‑GAATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACCGAAAGCGATGCAGAAATCGTTCGGCGGCAACTA  >  NC_000913/4004887‑4005063

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑