Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A1 F1 I2 R1	882	254.4	12957588	98.0%	12698436	93.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,013,772	+C	intergenic (‑53/‑246)	ysgA ← / → udp	hypothetical protein/uridine phosphorylase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,013,770	1	.	C	100.0%	652.3 / NA	187	intergenic (‑51/‑248)	ysgA/udp	hypothetical protein/uridine phosphorylase
Reads supporting (aligned to +/- strand): ref base . (0/0); new base C (95/92); total (95/92)

Only 100 of 198 total aligned reads displayed.

GCGTCGTCCGGGGTCTGAACGATGGTCGAAGCGAGAGGC‑ATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCGTA‑CCTTATAAAAATGTTGCGCAATGTTAACTA‑TAGTCAGCA‑TGCAACAAATCACATTGCCTGAATCGGCTCATCTTTTATGCAGTCCT  >  NC_000913/4013679‑4013856

  gtcgtcCGGGGTCTGAACGATGGTCGAAGCGAGAGGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCGTA‑cc                                                                                        >  1:1282231/1‑93 (MQ=255)

        cGGGGTCTGAACGATGGTCGAAGCGAGAGGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCGTACCCTtata                                                                                   <  1:39092/93‑1 (MQ=255)

        cGGGGTCTGAACGATGGTCGAAGCGAGAGGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCGTACCCTtata                                                                                   <  1:5606439/93‑1 (MQ=255)

        cGGGGTCTGAACGATGGTCGAAGCGAGAGGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCGTACCCTtata                                                                                   <  1:1495030/93‑1 (MQ=255)

        cGGGGTCTGAACGATGGTCGAAGCGAGAGGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCGTACCCTtata                                                                                   <  1:184882/93‑1 (MQ=255)

         ggggTCTGAACGATGGTCGAAGCGAGAGGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCGTACCCTTATaa                                                                                  >  1:4868499/1‑93 (MQ=255)

         ggggTCTGAACGATGGTCGAAGCGAGAGGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCGTACCCTTATaa                                                                                  <  2:5262180/93‑1 (MQ=255)

          gggTCTGAACGATGGTCGAAGCGAGAGGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCGTACCCTTATaaa                                                                                 >  1:152299/1‑93 (MQ=255)

            gTCTGAACGATGGTCGAAGCGAGAGGCGATGCAGCAGGTGCAAATCCAGTTTGTTGGGTTGTTGCCTTGGAATTCTCCTTA‑CCCTATTAAAAt                                                                              >  1:2559824/1‑93 (MQ=255)

            gTCTGAACGATGGTCGAAGCGAGAGGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCGTACCCTTATaaaaa                                                                               <  2:2599287/93‑1 (MQ=255)

            gTCTGAACGATGGTCGAAGCGAGAGGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCGTACCCTTATaaaaa                                                                               >  1:3648623/1‑93 (MQ=255)

             tCTGAACGATGGTCGAAGCGAGAGGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCGTACCCTTATAAAAAt                                                                              <  2:4810178/93‑1 (MQ=255)

             tCTGAACGATGGTCGAAGCGAGAGGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCGTACCCTTATAAAAAt                                                                              <  2:3487231/93‑1 (MQ=255)

               tGAACGATGGTCGAAGCGAGAGGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCGTACCCTTATAAAAATGt                                                                            >  2:5924485/1‑93 (MQ=255)

                gAACGATGGTCGAAGCGAGAGGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCGTACCCTTATAAAAATGtt                                                                           <  2:5433430/93‑1 (MQ=255)

                 aaCGATGGTCGAAGCGAGAGGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCGTACCCTTATAAAAATGTTg                                                                          <  1:5852756/93‑1 (MQ=255)

                 aaCGATGGACGAAGCGAGAGGCGATGAAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCGTACCCTTATAAAAATGTTg                                                                          <  2:3589628/93‑1 (MQ=255)

                  aCGATGGTCGAAGCGAGAGGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCGTACCCTTATAAAAATGTTgc                                                                         <  1:4059599/93‑1 (MQ=255)

                    gATGGTCGAAGCGAGAGGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCGTACCCTTATAAAAATGTTgcgc                                                                       >  1:3135127/1‑93 (MQ=255)

                       ggTCGAAGCGAGAGGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCGTACCCTTATAAAAATGTTGCGCAAt                                                                    <  2:5067831/93‑1 (MQ=255)

                          cGAAGCGAGAGGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCGTACCCTTATAAAAATGTTGCGCAATGtt                                                                 <  2:4382822/93‑1 (MQ=255)

                           gAAGCGAGAGGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCGTACCCTTATAAAAATGTTGCGCAATGTTa                                                                >  1:22699/1‑93 (MQ=255)

                             aGCGAGAGGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCGTACCCTTATAAAAATGTTGCGCAATGTTAAc                                                              >  1:3401084/1‑93 (MQ=255)

                             aGCGAGAGGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCGTACCCTTATAAAAATGTTGCGCAATGTTAAc                                                              <  1:1823076/93‑1 (MQ=255)

                              gCGAGAGGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCGTACCCTTATAAAAATGTTGCGCAATGTTAACt                                                             >  2:1535942/1‑93 (MQ=255)

                               cGAGAGGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCGTACCCTTATAAAAATGTTGCGCAATGTTAACta                                                            <  1:5350642/93‑1 (MQ=255)

                                gagaGGCGATGCAGCAGGTGCAAATCCAGATTGTTGTTTTGTTGCCATGGTATTCTCCGTACCCTTATAAAAATGTTGCGCAATGTTAACta‑t                                                          <  1:5416281/93‑1 (MQ=255)

                                gagaGGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCGTACCCTTATAAAAATGTTGCGCAATGTTAACta‑t                                                          >  2:4328069/1‑93 (MQ=255)

                                gagaGGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCGTACCCTTATAAAAATGTTGCGCAATGTTAACta‑t                                                          >  2:3456558/1‑93 (MQ=255)

                                    ggcgaTGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCGTACCCTTATAAAAATGTTGCGCAATGTTAACTA‑TAGTc                                                      <  2:2369669/89‑1 (MQ=255)

                                    ggcgaTGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCGTACCCTTATAAAAATGTTGCGCAATGTTAACTA‑TAGTc                                                      <  1:1072967/89‑1 (MQ=255)

                                     gcgaTGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCGTACCCTTATAAAAATGTTGCGCAATGTTAACTA‑TAGTCa                                                     <  1:1329491/90‑1 (MQ=255)

                                      cgaTGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCGTACCCTTATAAAAATGTTGCGCAATGTTAACTA‑TAGTCAg                                                    <  1:3741456/91‑1 (MQ=255)

                                       gaTGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCGTACCCTTATAAAAATGTTGCGCAATGTTAACTA‑TAGTCAGc                                                   >  1:5228797/2‑93 (MQ=255)

                                        aTGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCGTACCCTTATAAAAATGTTGCGCAATGTTAACTA‑TAGTCAGCa                                                  >  1:682634/1‑93 (MQ=255)

                                         tGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCGTACCCTTATAAAAATGTTGCGCAATGTTAACTA‑TAGTCAGCA‑t                                                <  2:3214710/93‑1 (MQ=255)

                                            agcagGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCGTACCCTTATAAAAATGTTGCGCAATGTTAACTA‑TAGTCAGCA‑TGca                                             >  1:447126/1‑93 (MQ=255)

                                                ggTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCGTACCCTTATAAAAATGTTGCGCAATGTTAACTA‑TAGTCAGCA‑TGcaacaa                                         <  1:3497024/93‑1 (MQ=255)

                                                 gTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCGTACCCTTATAAAAATGTTGCGCAATGTTAACTA‑TAGTCAGCA‑TGccacaat                                        >  1:4116263/1‑92 (MQ=255)

                                                 gTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCGTACCCTTATAAAAATGTTGCGCAATGTTAACTA‑TAGTCAGCA‑TGCAACaaa                                        >  1:4336568/1‑93 (MQ=255)

                                                  tGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCGTACCCTTATAAAAATGTTGCGCAATGTTAACTA‑TAGTCAGCA‑TGCAACAAAt                                       >  1:2404163/1‑93 (MQ=255)

                                                  tGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCGTACCCTTATAAAAATGTTGCGCAATGTTAACTA‑TAGTCAGCA‑TGCAACAAAt                                       <  1:5135915/93‑1 (MQ=255)

                                                   gCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCGTACCCTTATAAAAATGTTGCGCAATGTTAACTA‑TAGTCAGCA‑TGCAACAAATc                                      >  1:6355020/1‑93 (MQ=255)

                                                   gCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCGTACCCTTATAAAAATGTTGCGCAATGTTAACTA‑TAGTCAGCA‑TGCAACAAATc                                      <  2:3109422/93‑1 (MQ=255)

                                                   gCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCGTACCCTTATAAAAATGTTGCGCAATGTTAACTA‑TAGTCAGCA‑TGCAACAAATc                                      >  2:3567884/1‑93 (MQ=255)

                                                    cAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCGTACCCTTATAAAAATGTTGCGCAATGTTAACTA‑TAGTCAGCA‑TGCAACAAATca                                     <  2:1229284/93‑1 (MQ=255)

                                                     aaaTCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCGTACCCTTATAAAAATGTTGCGCAATGTTAACTA‑TAGTCAGCA‑TGCAACAAATcac                                    >  1:1595551/1‑93 (MQ=255)

                                                      aaTCCAGATTGTTGTGTTGTTGCCCTGGAATTCTCCGTACCCTTATAAAAATGTTGCGCAATGTTAACTA‑TAGTCAGCA‑TGCAACAAATcaca                                   >  2:4255170/1‑93 (MQ=255)

                                                      aaTCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCGTACCCTTATAAAAATGTTGCGCAATGTTAACTA‑TAGTCAGCA‑TGCAACAAATcaca                                   <  1:1146513/93‑1 (MQ=255)

                                                      aaTCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCGTACCCTTATAAAAATGTTGCGCAATGTTAACTA‑TAGTCAGCA‑TGCAACAAATcaca                                   >  2:1759432/1‑93 (MQ=255)

                                                      aaTCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCGTACCCTTATAAAAATGTTGCGCAATGTTAACTA‑TAGTCAGCA‑TGCAACAAATcaca                                   >  2:3322494/1‑93 (MQ=255)

                                                       aTCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCGTACCCTTATAAAAATGTTGCGCAATGTTAACTA‑TAGTCAGCA‑TGCAACAAATCACAt                                  >  1:3098878/1‑93 (MQ=255)

                                                        tCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCGTACCCTTATAAAAATGTTGCGCAATGTTAACTA‑TAGTCAGCA‑TGCAACAAATCACAtt                                 >  2:6155078/1‑93 (MQ=255)

                                                        tCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCGTACCCTTATAAAAATGTTGCGCAATGTTAACTA‑TAGTCAGCA‑TGCAACAAATCACAtt                                 >  2:948208/1‑93 (MQ=255)

                                                        tCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCGTACCCTTATAAAAATGTTGCGCAATGTTAACTA‑TAGTCAGCA‑TGCAACAAATCACAtt                                 >  1:959836/1‑93 (MQ=255)

                                                        tCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCGTACCCTTATAAAAATGTTGCGCAATGTTAACTA‑TAGTCAGCA‑TGCAACAAATCACAtt                                 <  1:867615/93‑1 (MQ=255)

                                                        tCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCGTACCCTTATAAAAATGTTGCGCAATGTTAACTA‑TAGTCAGCA‑TGCAACAAATCACAtt                                 >  1:2741484/1‑93 (MQ=255)

                                                         ccAGATTGTTGTGTTGTTGCCATGGTATTCTCCGTACCCTTATAAAAATGTTGCGCAATGTTAACTA‑TAGTCAGCA‑TGCAACAAATCACATTg                                <  1:4269956/93‑1 (MQ=255)

                                                          cAGATTGTTGTGTTGTTGCCATGGTATTCTCCGTACCCTTATAAAAATGTTGCGCAATGTTAACTA‑TAGTCAGCA‑TGCAACAAATCACATTGc                               >  1:6194739/1‑93 (MQ=255)

                                                           aGATTGTTGTGTTGTTGCCATGGTATTCTCCGTACCCTTATAAAAATGTTGCGCAATGTTAACTA‑TAGTCAGCA‑TGCAACAAATCACATTGcc                              <  2:1376260/93‑1 (MQ=255)

                                                           aGATTGTTGTGTTGTTGCCATGGTATTCTCCGTACCCTTATAAAAATGTTGCGCAATGTTAACTA‑TAGTCAGCA‑TGCAACAAATCACATTGcc                              >  2:2705033/1‑93 (MQ=255)

                                                            gATTGTTGTGTTGTTGCCATGGTATTCTCCGTACCCTTATAAAAATGTTGCGCAATGTTAACTA‑TAGTCAGCA‑TGCAACAAATCACATTGCCt                             >  2:5852999/1‑93 (MQ=255)

                                                             aTTGTTGTGTTGTTGCCATGGTATTCTCCGTACCCTTATAAAAATGTTGCGCAATGTTAACTA‑TAGTCAGCA‑TGCAACAAATCACATTGCCTg                            >  1:640328/1‑93 (MQ=255)

                                                             aTTGTTGTGTTGTTGCCATGGTATTCTCCGTACCCTTATAAAAATGTTGCGCAATGTTAACTA‑TAGTCAGCA‑TGCAACAAATCACATTGCCTg                            >  1:817578/1‑93 (MQ=255)

                                                              ttgttgTGTTGTTGCCATGGTATTCTCCGTACCCTTATAAAAATGTTGCGCAATGTTAACTA‑TAGTCAGCA‑TGCAACAAATCACATTGCCTGa                           >  2:6095045/1‑93 (MQ=255)

                                                              ttgttgTGTTGTTGCCATGGTATTCTCCGTACCCTTATAAAAATGTTGCGCAATGTTAACTA‑TAGTCAGCA‑TGCAACAAATCACATTGCCTGa                           >  1:2018100/1‑93 (MQ=255)

                                                              ttgttgTGTTGTTGCCATGGTATTCTCCGTACCCTTATAAAAATGTTGCGCAATGTTAACTA‑TAGTCAGCA‑TGCAACAAATCACATTGCCTGa                           >  2:5408866/1‑93 (MQ=255)

                                                              ttgttgTGTTGTTGCCATGGTATTCTCCGTACCCTTATAAAAATGTTGCGCAATGTTAACTA‑TAGTCAGCA‑TGCAACAAATCACATTGCCTGa                           >  2:516457/1‑93 (MQ=255)

                                                               tgttgtgttgTTGCCATGGTATTCTCGTTACCCTTTTAAAATTGTGGCGC‑ATGTTTACTATTAGTC‑GCATTGCAAAAATTACCATTGCCtaat                          >  1:2885194/1‑90 (MQ=255)

                                                               tgttgtgttgTTGCCATGGTATTCTCCGTACCCTTATAAAAATGTTGCGCAATGTTAACTA‑TAGTCAGCA‑TGCAACAAATCACATTGCCTGaa                          <  2:826579/93‑1 (MQ=255)

                                                               tgttgtgttgTTGCCATGGTATTCTCCGTACCCTTATAAAAATGTTGCGCAATGTTAACTA‑TAGTCAGCA‑TGCAACAAATCACATTGCCTGaa                          >  1:1434593/1‑93 (MQ=255)

                                                               tgttgtgttgTTGCCATGGTATTCTCCGTACCCTTATAAAAATGTTGCGCAATGTTAACTA‑TAGTCAGCA‑TGCAACAAATCACATTGCCTGaa                          <  1:2688254/93‑1 (MQ=255)

                                                                 ttgtgttgtTGCCATGGTATTCTCCGTACCCTTATAAAAATGTTGCGCAATGTTAACTA‑TAGTCAGCA‑TGCAACAAATCACATTGCCTGAATc                        <  1:4822808/93‑1 (MQ=255)

                                                                 ttgtgttgtTGCCATGGTATTCTCCGTACCCTTATAAAAATGTTGCGCAATGTTAACTA‑TAGTCAGCA‑TGCAACAAATCACATTGCCTGAATc                        <  1:1920947/93‑1 (MQ=255)

                                                                 ttgtgttgtTGCCATGGTATTCTCCGTACCCTTATAAAAATGTTGCGCAATGTTAACTA‑TAGTCAGCA‑TGCAACAAATCACATTGCCTGAATc                        <  2:5724935/93‑1 (MQ=255)

                                                                 ttgtgttgtTGCCATGGTATTCTCCGTACCCTTATAAAAATGTTGCGCAATGTTAACTA‑TAGTCAGCA‑TGCAACAAATCACATTGCCTGAATc                        <  2:299459/93‑1 (MQ=255)

                                                                     gttgttGCCATGGTATTCTCCGTACCCTTATAAAAATGTTGCGCAATGTTAACTA‑TAGTCAGCA‑TGCAACAAATCACATTGCCTGAATCGGCt                    >  1:6348192/1‑93 (MQ=255)

                                                                      ttgttgCCATGGTATTCTCCGTACCCTTATAAAAATGTTGCGCAATGTTAACTA‑TAGTCAGCA‑TGCAACAAATCACATTGCCTGAATCGGCTc                   >  1:3315838/1‑93 (MQ=255)

                                                                      ttgttgCCATGGTATTCTCCGTACCCTTATAAAAATGTTGCGCAATGTTAACTA‑TAGTCAGCA‑TGCAACAAATCACATTGCCTGAATCGGCTc                   >  1:5987432/1‑93 (MQ=255)

                                                                         ttgCCATGGTATTCTCCGTACCCTTATAAAAATGTTGCGCAATGTTAACTA‑TAGTCAGCA‑TGCAACAAATCACATTGCCTGAATCGGCTCATc                <  1:1737368/93‑1 (MQ=255)

                                                                           gCCATGGTATTCTCCGTACCCTTATAAAAATGTTGCGCAATGTTAACTA‑TAGTCAGCA‑TGCAACAAATCACATTGCCTGAATCGGCTCATCtt              >  1:4664903/1‑93 (MQ=255)

                                                                           gCCATGGTATTCTCCGTACCCTTATAAAAATGTTGCGCAATGTTAACTA‑TAGTCAGCA‑TGCAACAAATCACATTGCCTGAATCGGCTCATCtt              <  2:1185425/93‑1 (MQ=255)

                                                                           gCCATGGTATTCTCCGTACCCTTATAAAAATGTTGCGCAATGTTAACTA‑TAGTCAGCA‑TGCAACAAATCACATTGCCTGAATCGGCTCATCtt              >  2:1791035/1‑93 (MQ=255)

                                                                            ccATGGTATTCTCCGTACCCTTATAAAAATGTTGCGCAATGTTAACTA‑AAGTCAGCA‑TGCAACAAATCACATTGCCTGAATCGGCTCATCttt             <  1:2094396/93‑1 (MQ=255)

                                                                             cATGGTATTCTCCGTACCCTTATAAAAATGTTGCGCAATGTTAACTA‑TAGTCAGCA‑TGCAACAAATCACATTGCCTGAATCGGCTCATCtttt            >  2:1966739/1‑93 (MQ=255)

                                                                              aTGGTATTCTCCGTACCCTTATAAAAATGTTGCGCAATGTTAACTA‑TAGTCAGCA‑TGCAACAAATCACATTGCCTGAATCGGCTCATCTTTTa           >  2:4086994/1‑93 (MQ=255)

                                                                              aTGGTATTCTCCGTACCCTTATAAAAATGTTGCGCAATGTTAACTA‑TAGTCAGCA‑TGCAACAAATCACATTGCCTGAATCGGCTCATCTTTTa           >  2:4642205/1‑93 (MQ=255)

                                                                               tGGTATTCTCCGTACCCTTATAAAAATGTTGCGCAATGTTAACTA‑TAGTCAGCA‑TGCAACAAATCACATTGCCTGAATCGGCTCATCTTTTAt          >  2:3413967/1‑93 (MQ=255)

                                                                               tGGTATTCTCCGTACCCTTATAAAAATGTTGCGCAATGTTAACTA‑TAGTCAGCA‑TGCAACAAATCACATTGCCTGAATCGGCTCATCTTTTAt          <  1:3597072/93‑1 (MQ=255)

                                                                                ggTATTCTCCGTACCCTTATAAAAATGTTGCGCAATGTTAACTA‑TAGTCAGCA‑TGCAACAAATCACATTGCCTGAATCGGCTCATCTTTTATg         >  1:4504779/1‑93 (MQ=255)

                                                                                 gTATTCTCCGTACCCTTATAAAAATGTTGCGCAATGTTAACTA‑TAGTCAGCA‑TGCAACAAATCACATTGCCTGAATCGGCTCATCTTTTATGc        <  2:3951895/93‑1 (MQ=255)

                                                                                  tATTCTCCGTACCCTTATAAAAATGTTGCGCAATGTTAACTA‑TAGTCAGCA‑TGCAACAAATCACATTGCCTGAATCGGCTCATCTTTTATGCa       >  1:5426017/1‑93 (MQ=255)

                                                                                  tATTCTCCGTACCCTTATAAAAATGTTGCGCAATGTTAACTA‑TAGTCAGCA‑TGCAACAAATCACATTGCCTGAATCGGCTCATCTTTTATGCa       <  2:1711343/93‑1 (MQ=255)

                                                                                  tATTCTCCGTACCCTTATAAAAATGTTGCGCAATGTTAACTA‑TAGTCAGCA‑TGCAACAAATCACATTGCCTGAATCGGCTCATCTTTTATGCa       >  2:1614794/1‑93 (MQ=255)

                                                                                    ttCTCCGTACCCTTATAAAAATGTTGCGCAATGTTAACTA‑TAGTCAGCA‑TGCAACAAATCACATTGCCTGAATCGGCTCATCTTTTATGCAGt     <  2:6019539/93‑1 (MQ=255)

                                                                                    ttCTCCGTACCCTTATAAAAATGTTGCGCAATGTTAACTA‑TAGTCAGCA‑TGCAACAAATCACATTGCCTGAATCGGCTCATCTTTTATGCAGt     >  2:634692/1‑93 (MQ=255)

                                                                                    ttCTCCGTACCCTTATAAAAATGTTGCGCAATGTTAACTA‑TAGTCAGCA‑TGCAACAAATCACATTGCCTGAATCGGCTCATCTTTTATGCAGt     <  2:773446/93‑1 (MQ=255)

                                                                                    ttCTCCGTACCCTTATAAAAATGTTGCGCAATGTTAACTA‑TAGTCAGCA‑TGCAACAAATCACATTGCCTGAATCGGCTCATCTTTTATGCAGt     >  1:4964563/1‑93 (MQ=255)

                                                                                     tctcCGTACCCTTATAAAAATGTTGCGCAATGTTAACTA‑TAGTCAGCA‑TGCAACAAATCACATTGCCTGAATCGGCTCATCTTTTATGCAGTc    <  1:3330073/93‑1 (MQ=255)

                                                                                      ctcCGTACCCTTATAAAAATGTTGCGCAATGTTAACTA‑TAGTCAGCA‑TGCAACAAATCACATTGCCTGAATCGGCTCATCTTTTATGCAGTcc   <  1:6141621/93‑1 (MQ=255)

GCGTCGTCCGGGGTCTGAACGATGGTCGAAGCGAGAGGC‑ATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCGTA‑CCTTATAAAAATGTTGCGCAATGTTAACTA‑TAGTCAGCA‑TGCAACAAATCACATTGCCTGAATCGGCTCATCTTTTATGCAGTCCT  >  NC_000913/4013679‑4013856

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 31 ≤` `ATCG/ATCG` `< 35 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

BRESEQ :: bam2aln output

CGTCGTCCGGGGTCTGAACGATGGTCGAAGCGAGA‑GGC‑ATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCGTA‑CCTTATAAAAATGTTGCGCAATGTTAACTATAGTCAGCATGCAACAAATCACATTGCCTGAATCGGCTCATCTTTTATGCAGTCCTGCAGAATGA  >  NC_000913/4013680‑4013865

CGTCGTCCGGGGTCTGAACGATGGTCGAAGCGAGA‑GGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCGTA‑CC                                                                                               >  SRR4302537.953206/1‑94 (MQ=60)

CGTCGTCCGGGGTCTGAACGATGGTCGAAGCGAGA‑GGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCGTA‑CC                                                                                               >  SRR4302537.1291145/1‑94 (MQ=60)

 GTCGTCCGGGGTCTGAACGATGGTCGAAGCGAGA‑GGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCGTA‑CCC                                                                                              >  SRR4302537.5286055/1‑94 (MQ=60)

  TCGTCCGGGGTCTGAACGATGGTCGAAGCGAGA‑GGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCGTA‑CCCT                                                                                             <  SRR4302537.1525233/94‑1 (MQ=60)

      CCGGGGTCTGAACGATGGTCGAAGCGAGA‑GGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCGTACCCTTATA                                                                                          <  SRR4302537.6247153/94‑1 (MQ=60)

       CGGGGTCTGAACGATGGTCGAAGCGAGA‑GGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCGTACCCTTATAA                                                                                         <  SRR4302537.186200/94‑1 (MQ=60)

       CGGGGTCTGAACGATGGTCGAAGCGAGA‑GGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCGTACCCTTATAA                                                                                         >  SRR4302537.4902093/1‑94 (MQ=60)

       CGGGGTCTGAACGATGGTCGAAGCGAGA‑GGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCGTACCCTTATAA                                                                                         <  SRR4302537.4099067/94‑1 (MQ=60)

       CGGGGTCTGAACGATGGTCGAAGCGAGA‑GGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCGTACCCTTATAA                                                                                         <  SRR4302537.39388/94‑1 (MQ=60)

       CGGGGTCTGAACGATGGTCGAAGCGAGA‑GGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCGTACCCTTATAA                                                                                         <  SRR4302537.5644769/94‑1 (MQ=60)

       CGGGGTCTGAACGATGGTCGAAGCGAGA‑GGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCGTACCCTTATAA                                                                                         <  SRR4302537.1505452/94‑1 (MQ=60)

        GGGGTCTGAACGATGGTCGAAGCGAGA‑GGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCGTACCCTTATAAA                                                                                        >  SRR4302537.153387/1‑94 (MQ=60)

        GGGGTCTGAACGATGGTCGAAGCGAGA‑GGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTACTCTCCGTACCCTTATAAA                                                                                        >  SRR4302537.5644643/1‑94 (MQ=60)

          GGTCTGAACGATGGTCGAAGCGAGA‑GGCGATGCAGCAGGTGCAAATCCAGTTTGTTGGGTTGTTGCCTTGGAATTCTCCTTA‑CCCTATTAAAAT                                                                                     >  SRR4302537.2577763/1‑94 (MQ=57)

          GGTCTGAACGATGGTCGAAGCGAGA‑GGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCGTACCCTTATAAAAA                                                                                      >  SRR4302537.3674309/1‑94 (MQ=60)

           GTCTGAACGATGGTCGAAGCGAGA‑GGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCGTACCCTTATAAAAAT                                                                                     <  SRR4302537.14541/94‑1 (MQ=60)

           GTCTGAACGATGGTCGAAGCGAGA‑GGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCGTACCCTTATAAAAAT                                                                                     <  SRR4302537.5421072/94‑1 (MQ=60)

            TCTGAACGATGGTCGAAGCGAGA‑GGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCGTACCCTTATAAAAATG                                                                                    <  SRR4302537.2755427/94‑1 (MQ=60)

             CTGAACGATGGTCGAAGCGAGA‑GGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCGTACCCTTATAAAAATGT                                                                                   >  SRR4302537.4413042/1‑94 (MQ=60)

             CTGAACGATGGTCGAAGCGAGA‑GGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCGTACCCTTATAAAAATGT                                                                                   >  SRR4302537.4638329/1‑94 (MQ=60)

                AACGATGGTCGAAGCGAGA‑GGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCGTACCCTTATAAAAATGTTGC                                                                                <  SRR4302537.5892906/94‑1 (MQ=60)

                AACGATGGTCGAAGCGAGA‑GGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCGTACCCTTATAAAAATGTTGC                                                                                >  SRR4302537.3343421/1‑94 (MQ=60)

                 ACGATGGTCGAAGCGAGA‑GGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCGTACCCTTATAAAAATGTTGCG                                                                               <  SRR4302537.4087971/94‑1 (MQ=60)

                 ACGATGGTCGAAGCGAGA‑GGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCGTACCCTTATAAAAATGTTGCG                                                                               <  SRR4302537.2244150/94‑1 (MQ=60)

                  CGATGGTCGAAGCGAGAGGGG‑ATGCAGCGGGTGCAAACCCAGATTGTTGGTGTGTTGACATGGTATTCTCCGTACCCTTATCAAAATGTTGCGC                                                                              >  SRR4302537.3157130/1‑94 (MQ=30)

                  CGATGGTCGAAGCGAGA‑GGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCGTACCCTTATAAAAATGTTGCGC                                                                              >  SRR4302537.3157131/1‑94 (MQ=60)

                  CGATGGTCGAAGCGAGA‑GGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCGTACCCTTATAAAAATGTTGCGC                                                                              <  SRR4302537.1734314/94‑1 (MQ=60)

                     TGGTCGAAGCGAGA‑GGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCGTACCCTTATAAAAATGTTGCGCAAT                                                                           <  SRR4302537.3639197/94‑1 (MQ=60)

                       GTCGAAGCGAGA‑GGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCGTACCCTTATAAAAATGTTGCGCAATGT                                                                         <  SRR4302537.4747519/94‑1 (MQ=60)

                       GTCGAAGCGAGA‑GGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCGTACCCTTATAAAAATGTTGCGCAATGT                                                                         >  SRR4302537.1835146/1‑94 (MQ=60)

                        TCGAAGCGAGA‑GGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCGTACCCTTATAAAAATGTTGCGCAATGTT                                                                        <  SRR4302537.3917439/94‑1 (MQ=60)

                         CGAAGCGAGA‑GGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCGTACCCTTATAAAAATGTTGCGCAATGTTA                                                                       <  SRR4302537.4211302/94‑1 (MQ=60)

                         CGAAGCGAGA‑GGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCGTACCCTTATAAAAATGTTGCGCAATGTTA                                                                       >  SRR4302537.22874/1‑94 (MQ=60)

                           AAGCGAGA‑GGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCGTACCCTTATAAAAATGTTGCGCAATGTTAAC                                                                     >  SRR4302537.3425172/1‑94 (MQ=60)

                            AGCGAGA‑GGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCGTACCCTTATAAAAATGTTGCGCAATGTTAACT                                                                    <  SRR4302537.1835783/94‑1 (MQ=60)

                              CGAGA‑GGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCGTACCCTTATAAAAATGTTGCGCAATGTTAACTAT                                                                  <  SRR4302537.142381/94‑1 (MQ=60)

                              CGAGA‑GGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCGTACCCTTATAAAAATGTTGCGCAATGTTAACTAT                                                                  <  SRR4302537.5387298/94‑1 (MQ=60)

                               GAGA‑GGCGATGCAGCAGGTGCAAATCCAGATTGTTGTTTTGTTGCCATGGTATTCTCCGTACCCTTATAAAAATGTTGCGCAATGTTAACTATA                                                                 <  SRR4302537.5453208/94‑1 (MQ=60)

                                AGA‑GGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCGTACCCTTATAAAAATGTTGCGCAATGTTAACTATAG                                                                <  SRR4302537.2050303/94‑1 (MQ=60)

                                 GA‑GGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCGTACCCTTATAAAAATGTTGCGCAATGTTAACTATAGT                                                               <  SRR4302537.980855/94‑1 (MQ=60)

                                  A‑GGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCGTACCCTTATAAAAATGTTGCGCAATGTTAACTATAGTC                                                              <  SRR4302537.5228823/94‑1 (MQ=60)

                                  A‑GGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCGTACCCTTATAAAAATGTTGCGCAATGTTAACTATAGTC                                                              <  SRR4302537.1795742/94‑1 (MQ=60)

                                  A‑GGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCGTACCCTTATAAAAATGTTGCGCAATGTTAACTATAGTC                                                              <  SRR4302537.1987321/94‑1 (MQ=60)

                                    GGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCGTACCCTTATAAAAATGTTGCGCAATGTTAACTATAGTCA                                                             <  SRR4302537.1080361/94‑1 (MQ=60)

                                    GGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCGTACCCTTATAAAAATGTTGCGCAATGTTAACTATAGTCA                                                             >  SRR4302537.6345007/1‑94 (MQ=60)

                                    GGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCGTACCCTTATAAAAATGTTGCGCAATGTTAACTATAGTCA                                                             <  SRR4302537.2007470/94‑1 (MQ=60)

                                    GGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCGTACCCTTATAAAAATGTTGCGCAATGTTAACTATAGTCA                                                             <  SRR4302537.2869334/94‑1 (MQ=60)

                                    GGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCGTACCCTTATAAAAATGTTGCGCAATGTTAACTATAGTCA                                                             <  SRR4302537.4876519/94‑1 (MQ=60)

                                     gcgATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCGTACCCTTATAAAAATGTTGCGCAATGTTAACTATAGTCAG                                                            <  SRR4302537.1338759/91‑1 (MQ=60)

                                     gcgATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCGTACCCTTATAAAAATGTTGCGCAATGTTAACTATAGTCAG                                                            <  SRR4302537.3133000/91‑1 (MQ=60)

                                      cgATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCGTACCCTTATAAAAATGTTGCGCAATGTTAACTATAGTCAGC                                                           <  SRR4302537.3767734/92‑1 (MQ=60)

                                      cgATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCGTACCCTTATAAAAATGTTGCGCAATGTTAACTATAGTCAGC                                                           >  SRR4302537.5264756/3‑94 (MQ=60)

                                      G‑ATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCGTACCCTTATAAAAATGTTGCGCAATGTTAACTATAGTCAGCA                                                          >  SRR4302537.687313/1‑94 (MQ=60)

                                         TGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCGTACCCTTATAAAAATGTTGCGCAATGTTAACTATAGTCAGCATG                                                        >  SRR4302537.249699/1‑94 (MQ=60)

                                           CAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCGTACCCTTATAAAAATGTTGCGCAATGTTAACTATAGTCAGCATGCA                                                      >  SRR4302537.450227/1‑94 (MQ=60)

                                            AGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCGTACCCTTATAAAAATGTTGCGCAATGTTAACTATAGTCAGCATGCAA                                                     <  SRR4302537.6412197/94‑1 (MQ=60)

                                               AGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCGTACCCTTATAAAAATGTTGCGCAATGTTAACTATAGTCAGCATGCAACAA                                                  >  SRR4302537.2695038/1‑94 (MQ=60)

                                                GGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCGTACCCTTATAAAAATGTTGCGCAATGTTAACTATAGTCAGCATGCCACAAT                                                 >  SRR4302537.4145014/1‑94 (MQ=60)

                                                GGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCGTACCCTTATAAAAATGTTGCGCAATGTTAACTATAGTCAGCATGCAACAAA                                                 <  SRR4302537.3521764/94‑1 (MQ=60)

                                                GGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCGTACCCTTATAAAAATGTTGCGCAATGTTAACTATAGTCAGCATGCAACAAA                                                 >  SRR4302537.4366675/1‑94 (MQ=60)

                                                GGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCGTACCCTTATAAAAATGTTGCGCAATGTTAACTATAGTCAGCATGCAACAAA                                                 <  SRR4302537.4344699/94‑1 (MQ=60)

                                                 GTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCGTACCCTTATAAAAATGTTGCGCAATGTTAACTATAGTCAGCATGCAACAAAT                                                >  SRR4302537.2421087/1‑94 (MQ=60)

                                                  TGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCGTACCCTTATAAAAATGTTGCGCAATGTTAACTATAGTCAGCATGCAACAAATC                                               >  SRR4302537.6398399/1‑94 (MQ=60)

                                                  TGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCGTACCCTTATAAAAATGTTGCGCAATGTTAACTATAGTCAGCATGCAACAAATC                                               <  SRR4302537.5171302/94‑1 (MQ=60)

                                                    CAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCGTACCCTTATAAAAATGTTGCGCAATGTTAACTATAGTCAGCATGCAACAAATCAC                                             >  SRR4302537.1606701/1‑94 (MQ=60)

                                                      AATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCGTACCCTTATAAAAATGTTGCGCAATGTTAACTATAGTCAGCATGCAACAAATCACAT                                           <  SRR4302537.3325073/94‑1 (MQ=60)

                                                      AATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCGTACCCTTATAAAAATGTTGCGCAATGTTAACTATAGTCAGCATGCAACAAATCACAT                                           <  SRR4302537.1154486/94‑1 (MQ=60)

                                                      AATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCGTACCCTTATAAAAATGTTGCGCAATGTTAACTATAGTCAGCATGCAACAAATCACAT                                           >  SRR4302537.1565222/1‑94 (MQ=60)

                                                      AATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCGTACCCTTATAAAAATGTTGCGCAATGTTAACTATAGTCAGCATGCAACAAATCACAT                                           >  SRR4302537.3120506/1‑94 (MQ=60)

                                                       ATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCGTACCCTTATAAAAATGTTGCGCAATGTTAACTATAGTCAGCATGCAACAAATCACATT                                          >  SRR4302537.966455/1‑94 (MQ=60)

                                                       ATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCGTACCCTTATAAAAATGTTGCGCAATGTTAACTATAGTCAGCATGCAACAAATCACATT                                          >  SRR4302537.2760610/1‑94 (MQ=60)

                                                        TCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCGTACCCTTATAAAAATGTTGCGCAATGTTAACTATAGTCAGCATGCAACAAATCACATTG                                         <  SRR4302537.873570/94‑1 (MQ=60)

                                                         CCAGATTGTTGTGTTGTTGCCATGGTATTCTCCGTACCCTTATAAAAATGTTGCGCAATGTTAACTATAGTCAGCATGCAACAAATCACATTGC                                        >  SRR4302537.3504786/1‑94 (MQ=60)

                                                         CCAGATTGTTGTGTTGTTGCCATGGTATTCTCCGTACCCTTATAAAAATGTTGCGCAATGTTAACTATAGTCAGCATGCAACAAATCACATTGC                                        <  SRR4302537.4299623/94‑1 (MQ=60)

                                                         CCAGATTGTTGTGTTGTTGCCATGGTATTCTCCGTACCCTTATAAAAATGTTGCGCAATGTTAACTATAGTCAGCATGCAACAAATCACATTGC                                        >  SRR4302537.6237427/1‑94 (MQ=60)

                                                          CAGATTGTTGTGTTGTTGCCATGGTATTCTCCGTACCCTTATAAAAATGTTGCGCAATGTTAACTATAGTCAGCATGCAACAAATCACATTGCC                                       <  SRR4302537.4072294/94‑1 (MQ=60)

                                                          CAGATTGTTGTGTTGTTGCCATGGTATTCTCCGTACCCTTATAAAAATGTTGCGCAATGTTAACTATAGTCAGCATGCAACAAATCACATTGCC                                       >  SRR4302537.6195835/1‑94 (MQ=60)

                                                           AGATTGTTGTGTTGTTGCCATGGTATTCTCCGTACCCTTATAAAAATGTTGCGCAATGTTAACTATAGTCAGCATGCAACAAATCACATTGCCT                                      >  SRR4302537.192197/1‑94 (MQ=60)

                                                            GATTGTTGTGTTGTTGCCATGGTATTCTCCGTACCCTTATAAAAATGTTGCGCAATGTTAACTATAGTCAGCATGCAACAAATCACATTGCCTG                                     >  SRR4302537.667311/1‑94 (MQ=60)

                                                            GATTGTTGTGTTGTTGCCATGGTATTCTCCGTACCCTTATAAAAATGTTGCGCAATGTTAACTATAGTCAGCATGCAACAAATCACATTGCCTG                                     >  SRR4302537.644691/1‑94 (MQ=60)

                                                            GATTGTTGTGTTGTTGCCATGGTATTCTCCGTACCCTTATAAAAATGTTGCGCAATGTTAACTATAGTCAGCATGCAACAAATCACATTGCCTG                                     >  SRR4302537.823194/1‑94 (MQ=60)

                                                            GATTGTTGTGTTGTTGCCATGGTATTCTCCGTACCCTTATAAAAATGTTGCGCAATGTTAACTATAGTCAGCATGCAACAAATCACATTGCCTG                                     <  SRR4302537.1176791/94‑1 (MQ=60)

                                                             ATTGTTGTGTTGTTGCCATGGTATTCTCCGTACCCTTATAAAAATGTTGCGCAATGTTAACTATAGTCAGCATGCAACAAATCACATTGCCTGA                                    >  SRR4302537.2032169/1‑94 (MQ=60)

                                                              TTGTTGTGTTGTTGCCATGGTATTCTCCGTACCCTTATAAAAATGTTGCGCAATGTTAACTATAGTCAGCATGCAACAAATCACATTGCCTGAA                                   >  SRR4302537.1444571/1‑94 (MQ=60)

                                                               TGTTGTGTTGTTGCCATGGTATTCTCCGTACCCTTATAAAAATGTTGCGCAATGTTAACTATAGTCAGCATGCAACAAATCACATTGCCTGAAT                                  <  SRR4302537.831972/94‑1 (MQ=60)

                                                               TGTTGTGTTGTTGCCATGGTATTCTCCGTACCCTTATAAAAATGTTGCGCAATGTTAACTATAGTCAGCATGCAACAAATCACATTGCCTGAAT                                  <  SRR4302537.5766672/94‑1 (MQ=60)

                                                               TGTTGTGTTGTTGCCATGGTATTCTCCGTACCCTTATAAAAATGTTGCGCAATGTTAACTATAGTCAGCATGCAACAAATCACATTGCCTGAAT                                  <  SRR4302537.2707016/94‑1 (MQ=60)

                                                                GTTGTGTTGTTGCCATGGTATTCTCCGTACCCTTATAAAAATGTTGCGCAATGTTAACTATAGTCAGCATGCAACAAATCACATTGCCTGAATC                                 >  SRR4302537.5453771/1‑94 (MQ=60)

                                                                GTTGTGTTGTTGCCATGGTATTCTCCGTACCCTTATAAAAATGTTGCGCAATGTTAACTATAGTCAGCATGCAACAAATCACATTGCCTGAATC                                 >  SRR4302537.3548683/1‑94 (MQ=60)

                                                                 TTGTGTTGTTGCCATGGTATTCTCCGTACCCTTATAAAAATGTTGCGCAATGTTAACTATAGTCAGCATGCAACAAATCACATTGCCTGAATCG                                <  SRR4302537.4856120/94‑1 (MQ=60)

                                                                 TTGTGTTGTTGCCATGGTATTCTCCGTACCCTTATAAAAATGTTGCGCAATGTTAACTATAGTCAGCATGCAACAAATCACATTGCCTGAATCG                                <  SRR4302537.1934289/94‑1 (MQ=60)

                                                                  TGTGTTGTTGCCATGGTATTCTCCGTACCCTTATAAAAATGTTGCGCAATGTTAACTATAGTCAGCATGCAACAAATCACATTGCCTGAATCGG                               >  SRR4302537.5071805/1‑94 (MQ=60)

                                                                    TGTTGTTGCCATGGTATTCTCCGTACCCTTATAAAAATGTTGCGCAATGTTAACTATAGTCAGCATGCAACAAATCACATTGCCTGAATCGGCT                             >  SRR4302537.6391546/1‑94 (MQ=60)

                                                                     GTTGTTGCCATGGTATTCTCCGTACCCTTATAAAAATGTTGCGCAATGTTAACTATAGTCAGCATGCAACAAATCACATTGCCTGAATCGGCTC                            <  SRR4302537.3026630/94‑1 (MQ=60)

                                                                     GTTGTTGCCATGGTATTCTCCGTACCCTTATAAAAATGTTGCGCAATGTTAACTATAGTCAGCATGCAACAAATCACATTGCCTGAATCGGCTC                            >  SRR4302537.6028551/1‑94 (MQ=60)

                                                                     GTTGTTGCCATGGTATTCTCCGTACCCTTATAAAAATGTTGCGCAATGTTAACTATAGTCAGCATGCAACAAATCACATTGCCTGAATCGGCTC                            >  SRR4302537.3339292/1‑94 (MQ=60)

                                                                      TTGTTGCCATGGTATTCTCCGTACCCTTATAAAAATGTTGCGCAATGTTAACTATAGTCAGCATGCAACAAATCACATTGCCTaattcgcttat                           >  SRR4302537.4750884/1‑83 (MQ=60)

                                                                      TTGTTGCCATGGTATTCTCCGTACCCTTATAAAAATGTTGCGCAATGTTAACTATAGTCAGCATGCAACAAATCACATTGCCTGAATCGGCTCA                           >  SRR4302537.491643/1‑94 (MQ=60)

                                                                         TTGCCATGGTATTCTCCGTACCCTTATAAAAATGTTGCGCAATGTTAACTATAGTCAGCATGCAACAAATCACATTGCCTGAATCGGCTCATCT                        <  SRR4302537.1749486/94‑1 (MQ=60)

                                                                          TGCCATGGTATTCTCCGTACCCTTATAAAAATGTTGCGCAATGTTAACTATAGTCAGCATGCAACAAATCACATTGCCTGAATCGGCTCATCTT                       >  SRR4302537.4697178/1‑94 (MQ=60)

                                                                          TGCCATGGTATTCTCCGTACCCTTATAAAAATGTTGCGCAATGTTAACTATAGTCAGCATGCAACAAATCACATTGCCTGAATCGGCTCATCTT                       >  SRR4302537.4525151/1‑94 (MQ=60)

                                                                            CCATGGTATTCTCCGTACCCTTATAAAAATGTTGCGCAATGTTAACTATAGTCAGCATGCAACAAATCACATTGCCTGAATCGGCTCATCTTTT                     >  SRR4302537.2365989/1‑94 (MQ=60)

                                                                            CCATGGTATTCTCCGTACCCTTATAAAAATGTTGCGCAATGTTAACTAAAGTCAGCATGCAACAAATCACATTGCCTGAATCGGCTCATCTTTT                     <  SRR4302537.2109166/94‑1 (MQ=60)

                                                                             CATGGTATTCTCCGTACCCTTATAAAAATGTTGCGCAATGTTAACTATAGTCAGCATGCAACAAATCACATTGCCTGAATCGGCTCATCTTTTA                    <  SRR4302537.753038/94‑1 (MQ=60)

                                                                             CATGGTATTCTCCGTACCCTTATAAAAATGTTGCGCAATGTTAACTATAGTCAGCATGCAACAAATCACATTGCCTGAATCGGCTCATCTTTTA                    >  SRR4302537.3697213/1‑94 (MQ=60)

                                                                             CATGGTATTCTCCGTACCCTTATAAAAATGTTGCGCAATGTTAACTATAGTCAGCATGCAACAAATCACATTGCCTGAATCGGCTCATCTTTTA                    >  SRR4302537.3382001/1‑94 (MQ=60)

                                                                               TGGTATTCTCCGTACCCTTATAAAAATGTTGCGCAATGTTAACTATAGTCAGCATGCAACAAATCACATTGCCTGAATCGGCTCATCTTTTATG                  <  SRR4302537.5221540/94‑1 (MQ=60)

                                                                               TGGTATTCTCCGTACCCTTATAAAAATGTTGCGCAATGTTAACTATAGTCAGCATGCAACAAATCACATTGCCTGAATCGGCTCATCTTTTATG                  <  SRR4302537.3622427/94‑1 (MQ=60)

                                                                               TGGTATTCTCCGTACCCTTATAAAAATGTTGCGCAATGTTAACTATAGTCAGCATGCAACAAATCACATTGCCTGAATCGGCTCATCTTTTATG                  >  SRR4302537.4535930/1‑94 (MQ=60)

                                                                                 GTATTCTCCGTACCCTTATAAAAATGTTGCGCAATGTTAACTATAGTCAGCATGCAACAAATCACATTGCCTGAATCGGCTCATCTTTTATGCA                >  SRR4302537.5463007/1‑94 (MQ=60)

                                                                                   ATTCTCCGTACCCTTATAAAAATGTTGCGCAATGTTAACTATAGTCAGCATGCAACAAATCACATTGCCTGAATCGGCTCATCTTTTATGCAGT              >  SRR4302537.4998783/1‑94 (MQ=60)

                                                                                   ATTCTCCGTACCCTTATAAAAATGTTGCGCAATGTTAACTATAGTCAGCATGCAACAAATCACATTGCCTGAATCGGCTCATCTTTTATGCAGT              >  SRR4302537.5897876/1‑94 (MQ=60)

                                                                                    TTCTCCGTACCCTTATAAAAATGTTGCGCAATGTTAACTATAGTCAGCATGCAACAAATCACATTGCCTGAATCGGCTCATCTTTTATGCAGTC             <  SRR4302537.1837737/94‑1 (MQ=60)

                                                                                     TCTCCGTACCCTTATAAAAATGTTGCGCAATGTTAACTATAGTCAGCATGCAACAAATCACATTGCCTGAATCGGCTCATCTTTTATGCAGTCC            <  SRR4302537.3353635/94‑1 (MQ=60)

                                                                                      CTCCGTACCCTTATAAAAATGTTGCGCAATGTTAACTATAGTCAGCATGCAACAAATCACATTGCCTGAATCGGCTCATCTTTTATGCAGTCCT           <  SRR4302537.6183849/94‑1 (MQ=60)

                                                                                      CTCCGTACCCTTATAAAAATGTTGCGCAATGTTAACTATAGTCAGCATGCAACAAATCACATTGCCTGAATCGGCTCATCTTTTATGCAGTCCT           >  SRR4302537.1321752/1‑94 (MQ=60)

                                                                                      CTCCGTACCCTTATAAAAATGTTGCGCAATGTTAACTATAGTCAGCATGCAACAAATCACATTGCCTGAATCGGCTCATCTTTTATGCAGTCCT           <  SRR4302537.6183848/94‑1 (MQ=60)

                                                                                         CGTACCCTTATAAAAATGTTGCGCAATGTTAACTATAGTCAGCATGCAACAAATCACATTGCCTGAATCGGCTCATCTTTTATGCAGTCCTGCA        >  SRR4302537.177830/1‑94 (MQ=60)

                                                                                         CGTACCCTTATAAAAATGTTGCGCAATGTTAACTATAGTCAGCATGCAACAAATCACATTGCCTGAATCGGCTCATCTTTTATGCAGTCCTGCA        <  SRR4302537.3669349/94‑1 (MQ=60)

                                                                                          GTACCCTTATAAAAATGTTGCGCAATGTTAACTATAGTCAGCATGCAACAAATCACATTGCCTGAATCGGCTCATCTTTTATGCAGTCCTGCAG       >  SRR4302537.5024475/1‑94 (MQ=60)

                                                                                           tacCCTTATAAAAATGTTGCGCAATGTTAACTATAGTCAGCATGCAACAAATCACATTGCCTGAATCGGCTCATCTTTTATGCAGTCCTGCAGA      >  SRR4302537.4696/4‑94 (MQ=60)

                                                                                           tacCCTTATAAAAATGTTGCGCAATGTTAACTATAGTCAGCATGCAACAAATCACATTGCCTGAATCGGCTCATCTTTTATGCAGTCCTGCAGA      >  SRR4302537.1578073/4‑94 (MQ=60)

                                                                                            acCCTTATAAAAATGTTGCGCAATGTTAACTATAGTCAGCATGCAACAAATCACATTGCCTGAATCGGCTCATCTTTTATGCAGTCCTGCAGAA     >  SRR4302537.5336534/3‑94 (MQ=60)

                                                                                            acCCTTATAAAAATGTTGCGCAATGTTAACTATAGTCAGCATGCAACAAATCACATTGCCTGAATCGGCTCATCTTTTATGCAGTCCTGCAGAA     >  SRR4302537.3047779/3‑94 (MQ=60)

                                                                                            acCCTTATAAAAATGTTGCGCAATGTTAACTATAGTCAGCATGCAACAAATCACATTGCCTGAATCGGCTCATCTTTTATGCAGTCCTGCAGAA     >  SRR4302537.2103501/3‑94 (MQ=60)

                                                                                            C‑CCTTATAAAAATGTTGCGCAATGTTAACTATAGTCAGCATGCAACAAATCACATTGCCTGAATCGGCTCATCTTTTATGCAGTCCTGCAGAAT    <  SRR4302537.4838569/94‑1 (MQ=60)

                                                                                            C‑CCTTATAAAAATGTTGCGCAATGTTAACTATAGTCAGCATGCAACAAATCACATTGCCTGAATCGGCTCATCTTTTATGCAGTCCTGCAGAAT    >  SRR4302537.1985046/1‑94 (MQ=60)

                                                                                            C‑CCTTATAAAAATGTTGCGCAATGTTAACTATAGTCAGCATGCAACAAATCACATTGCCTGAATCGGCTCATCTTTTATGCAGTCCTGCAGAAT    <  SRR4302537.4620121/94‑1 (MQ=60)

                                                                                              CCTTATAAAAATGTTGCGCAATGTTAACTATAGTCAGCATGCAACAAATCACATTGCCTGAATCGGCTCATCTTTTATGCAGTCCTGCAGAATG   <  SRR4302537.1305945/94‑1 (MQ=60)

                                                                                              CCTTATAAAAATGTTGCGCAATGTTAACTATAGTCAGCATGCAACAAATCACATTGCCTGAATCGGCTCATCTTTTATGCAGTCCTGCAGAATG   >  SRR4302537.4917532/1‑94 (MQ=60)

                                                                                              CCTTATAAAAATGTTGCGCAATGTTAACTATAGTCAGCATGCAACAAATCACATTGCCTGAATCGGCTCATCTTTTATGCAGTCCTGCAGAATG   >  SRR4302537.1632810/1‑94 (MQ=60)

                                                                                               CTTATAAAAATGTTGCGCAATGTTAACTATAGTCAGCATGCAACAAATCACATTGCCTGAATCGGCTCATCTTTTATGCAGTCCTGCAGAATGA  >  SRR4302537.1845143/1‑94 (MQ=60)

                                                                                               CTTATAAAAATGTTGCGCAATGTTAACTATAGTCAGCATGCAACAAATCACATTGCCTGAATCGGCTCATCTTTTATGCAGTCCTGCAGAATGA  <  SRR4302537.5526267/94‑1 (MQ=60)

                                                                                               CTTATAAAAATGTTGCGCAATGTTAACTATAGTCAGCATGCAACAAATCACATTGCCTGAATCGGCTCATCTTTTATGCAGTCCTGCAGAATGA  >  SRR4302537.2366895/1‑94 (MQ=60)

CGTCGTCCGGGGTCTGAACGATGGTCGAAGCGAGA‑GGC‑ATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCGTA‑CCTTATAAAAATGTTGCGCAATGTTAACTATAGTCAGCATGCAACAAATCACATTGCCTGAATCGGCTCATCTTTTATGCAGTCCTGCAGAATGA  >  NC_000913/4013680‑4013865

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 0 ≤` `ATCG/ATCG` `< 21 ≤` `ATCG/ATCG` `< 30 ≤` `ATCG/ATCG` `< 35 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`