Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I2 R1 882 254.4 12957588 98.0% 12698436 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 4,034,652 C→T noncoding (1533/1542 nt) rrsA → 16S ribosomal RNA

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,034,6520CT100.0% 506.6 / NA 164noncoding (1533/1542 nt)rrsA16S ribosomal RNA
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (63/101);  total (63/101)

Only 100 of 164 total aligned reads displayed.

TTGTGATTCATGACTGGGGTGAAGTCGTAACAAGGTAACCGTAGGGGAACCTGCGGTTGGATCACCTCCTTACCTTAAAGAAGCGTTCTTTGAAGTGCTCACACAGATTGTCTGATGAAAATGAGCAGTAAAACCTCTACAGGCTTGTAGCT  >  NC_000913/4034590‑4034741
                                                              |                                                                                         
ttGTGATTCATGACTGGGGTGAAGTCGTAACAATGTAACCGTAGGGGAACCTGCGGTTGGATTACCTCCTTACCTTAAAGAAGCGTTCTTTGa                                                             <  2:3517627/93‑1 (MQ=9)
ttGTGATTCATGACTGGGGTGAAGTCGTAACAAGGTAACCGTAGGGGAACCTGCGGTTGGATTACCTCCTTACCTTAAAGAAGCGTTCTTTGa                                                             <  2:2526973/93‑1 (MQ=14)
ttGTGATTCAGGACTGGGGTGAAGTCGTAACAAGGTAACCGTAGGGGAACCTGCGGTTGGATTACGTCCTTACCTTAAAGAAGCGTTCTTTGa                                                             <  2:1536183/93‑1 (MQ=255)
 tgtgATTCATGACTGGGGTGAAGTCGTAACAAGGTAACCGTAGGGGAACCTGCGGTTGGATTCCCTCCTTACCTTAAAGAAGCGTTCTTTGaa                                                            <  2:4427084/93‑1 (MQ=12)
 tgtgATTCATGACTGGGGTGAAGTCGTAACAAGGTAACCGTAGGGGAACCTGCGGTTGGATTACCTCCTTACCTTAAAGAAGCGTTCTTTGaa                                                            <  2:5889401/93‑1 (MQ=17)
  gtgATTCATGACTGGGGTGAAGTCGTAACAAGGTAACCGTAGGGGAACCTGCGGTTGGATTACCTCCTTACCTTAAAGAAGCGTTCTTTGAAg                                                           <  2:2897996/93‑1 (MQ=18)
   tgATTCATGACTGGGGTGAAGTCGTAACAAGGTAACCGTAGGGGAACCTGCGGTTGGATTACCTCCTTACCTTAAAGAAGCGTTCTTTGAAGt                                                          <  2:2278896/93‑1 (MQ=18)
   tgATTCATGACTGGGGTGAAGTCGTAACAAGGTAACCGTAGGGGAACCTGCGGTTGGATTACCTCCTTACCTTAAAGAAGCGTTCTTTGAAGt                                                          <  1:4009875/93‑1 (MQ=18)
    gATTCATGACTGGGGTGAAGTCGTAACAAGGTAACCGTAGGGGAACCTGCGGTTGGATTACCTCCTTACCTTAAAGAAGCGTTCTTTGAAGTg                                                         >  2:3366803/1‑93 (MQ=18)
    gATTCATGACTGGGGTGAAGTCGTAACAAGGTAACCGTAGGGGAACCTGCGGTTGGATTACCTCCTTACCTTAAAGAAGCGTTCTTTGAAGTg                                                         <  2:5288816/93‑1 (MQ=18)
    gATTCATGACTGGGGTGAAGTCGTAACAAGGTAACCGTAGGGGAACCTGCGGTTGGATTACCTCCTTACCTTAAAGAAGCGCTCTTTGAAGTg                                                         >  2:2193204/1‑93 (MQ=16)
     aTTCATGACTGGGGTGAAGTCGTAACAAGGTAACCGTAGGGGAACCTGCGGTTGGATTACCTCCTTACCTTAAAGAAGCGTTCTTTGAAGTGc                                                        >  2:6229338/1‑93 (MQ=18)
     aTTCATGACTGGGGTGAAGTCGTAACAAGGTAACCGTAGGGGAACCTGCGGTTGGATTACCTCCTTACCTTAAAGAAGCGTTCTTTGAAGTGc                                                        >  1:4026567/1‑93 (MQ=18)
      ttCATGACTGGGGTGAAGTCGTAACAAGGTAACCGTAGGGGAACCTGCGGTTGGATTACCTCCTTACCTTAAAGAAGCGTTCTTTGAAGTGCt                                                       <  1:1872770/93‑1 (MQ=21)
      ttCATGACTGGGGTGAAGTCGTAACAAGGTAACCGTAGGGGAACCTGCGGTTGGATTACCTCCTTACCTTAAAGAAGCGTTCTTTGAAGTGCt                                                       >  1:117525/1‑93 (MQ=18)
       tCATGACTGGGGTGAAGTCGTAACAAGGTAACCGTAGGGGAACCTGCGGTTGGATTACCTCCTTACCTTAAAGAAGCGTTCTTTGAAGTGCTc                                                      >  2:2022790/1‑93 (MQ=18)
       tCATGACTGGGGTGAAGTCGTAACAAGGTAACCGTAGGGGAACCTGCGGTTGGATTACCTCCTTACCTTAAAGAAGCGTTCTTTGAAGTGCTc                                                      <  1:73368/93‑1 (MQ=21)
        cATGACTGGGGTGAAGTCGTAACAAGGTAACCGTAGGGGAACCTGCGGTTGGATTACCTCCTTACCTTAAAGAAGCGTTCTTTGAAGTGCTca                                                     <  2:116601/93‑1 (MQ=21)
        cATGACTGGGGTGAAGTCGTAACAAGGTAACCGTAGGGGAACCTGCGGTTGGATTACCTCCTTACCTTAAAGAAGCGTTCTTTGAAGTGCTca                                                     <  1:6254484/93‑1 (MQ=21)
         aTGACTGGGGTGAAGTCGTAACAAGGTAACCGTAGGGGAACCTGCGGTTGGATTACCTCCTTACCTTAAAGAAGCGTTCTTTGAAGTGCTcac                                                    <  1:724449/93‑1 (MQ=18)
          tGACTGGGGTGAAGTCGTAACAAGGTAACCGTAGGGGAACCTGCGGTTGGATTACCTCTTTACCTTAAAGAAGCGTTCTTTGAAGTGCTcaca                                                   <  2:4527317/93‑1 (MQ=255)
           gACTGGGGTGAAGTCGTAACAAGGTAACCGTAGGGGAACCTGCGGTTGGATTACCTCCTTACCTTAAAGAAGCGTTCTTTGAAGTGCTcacac                                                  <  2:5211030/93‑1 (MQ=21)
            aCTGGGGTGAAGTCGTAACAAGGTAACCGTAGGGGAACCTGCGGTTGGATTACCTCCTTACCTTAAAGAAGCGTTCTTTGAAGTGCTcacaca                                                 >  2:5977367/1‑93 (MQ=18)
            aCTGGGGTGAAGTCGTAACAAGGTAACCGTAGGGGAACCTGCGGTTGGATTACCTCCTTACCTTAAAGAAGCGTTCTTTGAAGTGCTcacaca                                                 <  1:4897920/93‑1 (MQ=18)
            aCTGGGGTGAAGTCGTAACAAGGTAACCGTAGGGGAACCTGCGGTTGGATTACCTCCTTACCTTAAAGAAGCGTTCTTTGAAGTGCTcacaca                                                 >  1:2140598/1‑93 (MQ=18)
             cTGGGGTGAAGTCGTAACAAGGTAACCGTAGGGGAACCTGCGGTTGGATTACCTCCTTACCTTAAAGAAGCGTTCTTTGAAGTGCTCACACAg                                                >  2:2473880/1‑93 (MQ=18)
              tGGGGTGAAGTCGTAACAAGGTAACCGTAGGGGAACCTGCGGTTGGATTACCTCCTTACCTTAAAGAAGCGTTCTTTGAAGTGCTCACACAGa                                               <  1:1230903/93‑1 (MQ=21)
               ggggTGAAGTCGTAACAAGGTAACCGTAGGGGAACCTGCGGTTGGATTACCTCCTTACCTTAAAGAAGCGTTCTTTGAAGTGCTCACACAGAt                                              >  1:4960710/1‑93 (MQ=18)
                gggTGAAGTCGTAACAAGGTAACCGTAGGGGAACCTGCGGTTGGATTACCTCCTTACCTTAAAGAAGCGTTCTTTGAAGTGCTCACACAGAtt                                             <  2:1932417/93‑1 (MQ=21)
                gggTGAAGTCGTAACAAGATAACCGTAGGGGAACCTGCGGTTGGATTACCTCCTTACCTTAAAGAAGCGTTCTTTGAAGTGCTCACACAGAtt                                             <  2:6112979/93‑1 (MQ=255)
                 ggTGAAGTCGTAACAAGGTAACCGTAGGGGAACCTGCGGTTGGATTACCTCCTTACCTTAAAGAAGCGTTCTTTGAAGTGCTCACACAGATTg                                            >  2:3540901/1‑93 (MQ=18)
                   tGAAGTCGTAACAAGGTAACCGTAGGGGAACCTGCGGTTGGATTACCTCCTTACCTTAAAGAAGCGTTCTTTGAAGTGCTCACAGAGATTGTc                                          >  1:3538957/1‑93 (MQ=16)
                   tGAAGTCGTAACAAGGTAACCGTAGGGGAACCTGCGGTTGGATTACCTCCTTACCTTAAAGAAGCGTTCTTTGAAGTGCTCACACAGATTGTc                                          <  1:4061241/93‑1 (MQ=21)
                   tGAAGTCGTAACAAGGTAACCGTAGGGGAACCTGCGGTTGGATTACCTCCTTACCTTAAAGAAGCGTTCTTTGAAGTGCTCACACAGATTGTc                                          <  2:1145759/93‑1 (MQ=18)
                   tGAAGTCGTAACAAGGTAACCGTAGGCGAACCTGCGGTTGGATTACCTCCTTACCTTAAAGAAGCGTTCTTTGAAGTGCTCACACAGATTGTc                                          <  1:4195169/93‑1 (MQ=255)
                    gAAGTCGTAACAAGGTAACCGTAGGGGAACCTGCGGTTGGATTACCTCCTTACCTTAAAGAAGCGTTCTTTGAAGTGCTCACACAGATTGTCt                                         >  1:294143/1‑93 (MQ=18)
                    gAAGTCGTAACAAGGTAACCGTAGGGGAACCTGCGGTTGGATTACCTCCTTACCTTAAAGAAGCGTTCTTTGAAGTGCTCACACAGATTGTCt                                         <  1:4605120/93‑1 (MQ=18)
                     aaGTCGCAACAAGGTAACCGTAGGGGAACCTGCGGTTGGATTACCTCCTTACCTTAAAGAAGCGTTCTTTGAAGTGCTCACACAGATTGTCtg                                        <  1:2282718/93‑1 (MQ=16)
                      aGTCGTAACAAGGTAACCGTAGGGGAACCTGCGGTTGGATTACCTCCTTACCTTAAAGAAGCGTTCTTTGAAGTGCTCACACAGATTGTCtga                                       <  1:4453702/93‑1 (MQ=21)
                       gTCGTAACAAGGTAACCGTAGGGGAACCTGCGGTTGGATTACCTCCTTACCTTAAAGAAGCGTTCTTTGAAGTGCTCACACAGATTGTCtgat                                      <  1:3377584/93‑1 (MQ=18)
                       gTCGTAACAAGGTAACCGTAGGGGAACCTGCGGTTGGATTACCTCCTTACCTTAAAGAAGCGTTCTTTGAAGTGCTCACACAGATTGTCtgat                                      <  1:5582936/93‑1 (MQ=21)
                       gTCGTAACAAGGTAACCGTAGGGGAACCTGCGGTTGGATTACCTCCTTACCTTAAAGAAGCGTTCTTTGAAGTGCTCACACAGATTGTCtgat                                      <  2:5732436/93‑1 (MQ=21)
                       gTCGTAACAAGGTAACCGTAGGGGAACCTGCGGTTGGATTACCTCCTTACCTTAAAGAAGCGTTCTTTGAAGTGCTCACACAGATTGTCtgat                                      >  2:4951794/1‑93 (MQ=18)
                       gTCGTAACAAGGTAACCGTAGGGGAACCTGCGGTTGGATTACCTCCTTACCTTAAAGAAGCGTTCTTTGAAGTGCTCACACAGATTGTCtgat                                      >  2:3245373/1‑93 (MQ=18)
                          gTAACAAGGTAACCGTAGGGGAACCTGCGGTTGGATTACCTCCTTACCTTAAAGAAGCGTTCTTTGAAGTGCTCACACAGATTGTCTGATGaa                                   >  2:6404159/1‑93 (MQ=255)
                          gTAACAAGGTAACCGTAGGGGAACCTGCGGTTGGATTACCTCCTTACCTTAAAGAAGCGTTCTTTGAAGTGCTCACACAGATTGTCTGATGaa                                   <  2:1094215/93‑1 (MQ=255)
                          gTAACAAGGTAACCGTAGGGGAACCTGCGGTTGGATTACCTCCTTACCTTAAAGAAGCGTTCTTTGAAGTGCTCACACAGATTGTCTGATGaa                                   >  1:1782172/1‑93 (MQ=37)
                           tAACAAGGTAACCGTAGGGGAACCTGCGGTTGGATTACCTCCTTACCTTAAAGAAGCGTTCTTTGAAGTGCTCACACAGATTGTCTGATGaaa                                  <  2:2660615/93‑1 (MQ=255)
                           tAACAAGGTAACCGTAGGGGAACCTGCGGTTGGATTACCTCCTTACCTTAAAGAAGCGTTCTTTGAAGTGCTCACACAGATTGTCTGATGaaa                                  <  2:3812710/93‑1 (MQ=37)
                            taCAAGGTAACCGTAGGGCAACCTGCGGTTGGATTACCTCCTTACCTTAAAGAAGCGTTCTTTGAAGTGCTCACACAGATTGTCTGATGaaaa                                 <  2:1834213/92‑1 (MQ=255)
                             aCAAGGTAACCGTAGGGGAACCTGCGGTTGGATTACCTCCTTACCTTAAAGAAGCGTTCTTTGAAGTGCTCACACAGATTGTCTGATGAAAAt                                >  1:136770/1‑93 (MQ=255)
                             aCAAGGTAACCGTAGGGGAACCTGCGGTTGGATTACCTCCTTACCTTAAAGAAGCGTTCTTTGAAGTGCTCACACAGATTGTCTGATGAAAAt                                >  1:6185680/1‑93 (MQ=255)
                              cAAGGTAACCGTAGGGGAACCTGCGGTTGGATTACCTCCTTACCTTAAAGAAGCGTTCTTTGAAGTGCTCACACAGATTGTCTGATGAAAATg                               <  1:546306/93‑1 (MQ=255)
                               aaGGTAACCGTAGGGGAACCTGCGGTTGGATTACCTCCTTACCTTAAAGAAGCGTTCTTTGAAGTGCTCACACAGATTGTCTGATGAAAATGa                              <  2:4678288/93‑1 (MQ=37)
                                aGGTAACCGTAGGGGAACCTGCGGTTGGATTACCTCCTTACCTTAAAGAAGCGTTCTTTGAAGTGCTCACACAGATTGTCTGATGAAAATGAg                             <  1:3389978/93‑1 (MQ=255)
                                 ggTAACCGTAGGGGAACCTGCGGTTGGATTACCTCCTTACCTTAAAGAAGCGTTCTTTGAAGTGCTCACACAGATTGTCTGATGAAAATGAGc                            <  1:725688/93‑1 (MQ=255)
                                 ggTAACCGTAGGGGAACCTGCGGTTGGATTACCTCCTTACCTTAAAGAAGCGTTCTTTGAAGTGCTCACACAGATTGTCTGATGAAAATGAGc                            >  2:5676197/1‑93 (MQ=255)
                                 ggTAACCGTAGGGGAACCTGCGGTTGGATTACCTCCTTACCTTAAAGAAGCGTTCTTTGAAGTGCTCACACAGATTGTCTGATGAAAATGAGc                            <  1:4344983/93‑1 (MQ=255)
                                  gTAACCGTAGGGGAACCTGCGGTTGGATTACCTCCTTACCTTAAAGAAGCGTTCTTTGAAGTGCTCACACAGATTGTCTGATGAAAATGAGCa                           <  2:3858128/93‑1 (MQ=255)
                                  gTAACCGTAGGGGAACCTGCGGTTGGATTACCTCCTTACCTTAAAGAAGCGTTCTTTGAAGTGCTCACACAGATTGTCTGATGAAAATGAGCa                           <  1:1539085/93‑1 (MQ=255)
                                  gTAACCGTAGGGGAACCTGCGGTTGGATTACCTCCTTACCTTAAAGAAGCGTTCTTTGAAGTGCTCACACAGATTGTCTGATGAAAATGAGCa                           <  1:5975979/93‑1 (MQ=255)
                                  gTAACCGTAGGGGAACCTGCGGTTGGATTACCTCCTTACCTTAAAGAAGCGTTCTTTGAAGTGCTCACACAGATTGTCTGATGAAAATGAGCa                           <  2:5960708/93‑1 (MQ=255)
                                    aaCCGTAGGGGAACCTGCGGTTGGATTACCTCCTTACCTTAAAGAAGCGTTCTTTGAAGTGCTCACACAGATTGTCTGATGAAAATGAGCAGt                         <  1:1130430/93‑1 (MQ=255)
                                     aCCGTAGGGGAACCTGCGGTTGGATTACCTCCTTACCTTAAAGAAGCGTTCTTTGAAGTGCTCACACAGATTGTCTGATGAAAATGAGCAGTa                        <  2:5431374/93‑1 (MQ=255)
                                     aCCGTAGGGGAACCTGCGGTTGGATTACCTCCTTACCTTAAAGAAGCGTTCTTTGAAGTGCTCACACAGATTGTCTGATGAAAATGAGCAGTa                        >  1:3813532/1‑93 (MQ=255)
                                     aCCGTAGGGGAACCTGCGGTTGGATTACCTCCTTACCTTAAAGAAGCGTTCTTTGAAGTGCTCACACAGATTGTCTGATGAAAATGAGCAGTa                        <  1:369807/93‑1 (MQ=255)
                                      ccGTAGGGGAACCTGCGGTTGGATTACCTCCTTACCTTAAAGAAGCGTTCTTTGAAGTGCTCACACAGATTGTCTGATGAAAATGAGCAGTaa                       >  1:753723/1‑93 (MQ=255)
                                      ccGTAGGGGAACCTGCGGTTGGATTACCTCCTTACCTTAAAGAAGCGTTCTTTGAAGTGCTCACACAGATTGTCTGATGAAAATGAGCAGTaa                       >  1:3211611/1‑93 (MQ=255)
                                        gTAGGGGAACCTGCGGTTGGATTACCTCCTTACCTTAAAGAAGCGTTCTTTGAAGTGCTCACACAGATTGTCTGATGAAAATGAGCAGTaaaa                     <  1:2461841/93‑1 (MQ=255)
                                        gTAGGGGAACCTGCGGTTGGATTACCTCCTTACCTTAAAGAAGCGTTCTTTGAAGTGCTCACACAGATTGTCTGATGAAAATGAGCAGTaaaa                     >  2:2400254/1‑93 (MQ=37)
                                        gTAGGGGAACCTGCGGTTGGATTACCTACTTACCTTAAAGAAGCGTTCTTTGAAGTGCTCACACAGATTGTCTGATGAAAATGAGCAGTaaaa                     >  1:6205120/1‑93 (MQ=255)
                                         tAGGGGAACCTGCGGTTGGATTACCTCCTTACCTTAAAGAAGCGTTCTTTGAAGTGCTCACACAGATTGTCTGATGAAAATGAGCAGTAAAAc                    <  1:3735978/93‑1 (MQ=255)
                                            gggAACCTGCGGTTGGATTACCTCCTTACCTTAAAGAAGCGTTCTTTGAAGTGCTCACACAGATTGTCTGATGAAAATGAGCAGTAAAACctc                 <  1:5078219/93‑1 (MQ=255)
                                              gacccTGCGGTTGGATTACCTCCTTACCTTAAAGAAGCGTTCTTTGAAGTGCTCACACAGATTGTCTGATGAAAATGAGCAGTAAAACCTCTa               <  2:6227607/90‑1 (MQ=255)
                                               aaCCTGCGGTTGGATTACCTCCTTACCTTAAAGAAGCGTTCTTTGAAGTGCTCACACAGATTGTCTGATGAAAATGAGCAGTAAAACCTCTAc              <  1:6086843/93‑1 (MQ=255)
                                                aCCTGCGGTTGGATTACCTCCTTACCTTAAAGAAGCGTTCTTTGAAGTGCTCACACAGATTGTCTGATGAAAATGAGCAGTAAAACCTCTACa             <  2:1619765/93‑1 (MQ=255)
                                                aCCTGCGGTTGGATTACCTCCTTACCTTAAAGAAGCGTTCTTTGAAGTGCTCACACAGATTGTCTGATGAAAATGAGCAGTAAAACCTCTACa             >  1:1582275/1‑93 (MQ=255)
                                                aCCTGCGGTTGGATTACCTCCTTACCTTAAAGAAGCGTTCTTTGAAGTGCTCACACAGATTGTCTGATGAAAATGAGCAGTAAAACCTCTACa             <  1:2692609/93‑1 (MQ=37)
                                                aCCTGCGGTTGGATTACCTCCTTACCTTAAAGAAGCGTTCTTTGAAGTGCTCACACAGATTGTCTGATGAAAATGAGCAGTAAAACCTCTACa             <  1:2011458/93‑1 (MQ=255)
                                                 ccTGCGGTTGGATTACCTCCTTACCTTAAAGAAGCGTTCTTTGAAGTGCTCACACAGATTGTCTGATGAAAATGAGCAGTAAAACCTCTACAg            <  2:3434380/93‑1 (MQ=255)
                                                  cTGCGGTTGGATTACCTCCTTACCTTAAAGAAGCGTTCTTTGAAGTGCTCACACAGATTGTCTGATGAAAATGAGCAGTAAAACCTCTACAgg           >  2:1339091/1‑93 (MQ=255)
                                                   tGCGGTTGGATTACCTCCTTACCTTAAAGAAGCGTTCTTTGAAGTGCTCACACAGATTGTCTGATGAAAATGAGCAGTAAAACCTCTACAGGc          >  1:3213746/1‑93 (MQ=255)
                                                   tGCGGTTGGATTACCTCCTTACCTTAAAGAAGCGTTCTTTGAAGTGCTCACACAGATTGTCTGATGAAAATGAGCAGTAAAACCTCTACAGGc          >  2:3064574/1‑93 (MQ=37)
                                                    gCGGTTGGATTACCTCCTTACCTTAAAGAAGCGTTCTTTGAAGTGCTCACACAGATTGTCTGATGAAAATGAGCAGTAAAACCTCTACAGGCt         <  1:1494750/93‑1 (MQ=255)
                                                    gCGGTTGGATTACCTCCTTACCTTAAAGAAGCGTTCTTTGAAGTGCTCACACAGATTGTCTGATGAAAATGAGCAGTAAAACCTCTACAGGCt         <  1:5033396/93‑1 (MQ=255)
                                                    gCGGTTGGATTACCTCCTTACCTTAAAGAAGCGTTCTTTGAAGTGCTCACACAGATTGTCTGATGAAAATGAGCAGTAAAACCTCTACAGGCt         <  2:3263408/93‑1 (MQ=37)
                                                     cGGTTGGATTACCTCCTTACCTTAAAGAAGCGTTCTTTGAAGTGCTCACACAGATTGTCTGATGAAAATGAGCAGTAAAACCTCTACAGGCtt        >  1:3295640/1‑93 (MQ=255)
                                                     cGGTTGGATTACCTCCTTACCTTAAAGAAGCGTTCTTTGAAGTGCTCACACAGATTGTCTGATGAAAATGAGCAGTAAAACCTCTACAGGCtt        >  2:1300067/1‑93 (MQ=255)
                                                       gTTGGATTACCTCCTTACCTTAAAGAAGCGTTCTTTGAAGTGCTCACACAGATTGTCTGATGAAAATTAGCAGTAAAACCTCTACAGGCTTGt      <  2:3178936/93‑1 (MQ=255)
                                                       gTTGGATTACCTCCTTACCTTAAAGAAGCGTTCTTTGAAGTGCTCACACAGATTGTCTGATGAAAATGAGCAGTAAAACCTCTACAGGCTTGt      <  2:5148509/93‑1 (MQ=255)
                                                       gTTGGATTACCTCCTTACCTTAAAGAAGCGTTCTTTGAAGTGCTCACACAGATTGTCTGATGAAAATGAGCAGTAAAACCTCTACAGGCTTGt      >  1:2482517/1‑93 (MQ=255)
                                                       gTTGGATTACCTCCTTACCTTAAAGAAGCGTTCTTTGAAGTGCTCACACAGATTGTCTGATGAAAATGAGCAGTAAAACCTCTACAGGCTTGt      >  1:1603157/1‑93 (MQ=255)
                                                       gTTGGATTACCTCCTTACCTTAAAGAAGCGTGCTTTGAAGTGCTCACACAGATTGTCTGATGAAAATGAGCAGTAAAACCTCTACAGGCTTGt      <  1:5420185/93‑1 (MQ=16)
                                                        ttGGATTACCTCCTTACCTTAAAGAAGCGTTCTTTGAAGTGCTCACACAGATTGTCTGATGAAAATGAGCAGTAAAACCTCTACAGGCTTGTa     >  2:3715004/1‑93 (MQ=255)
                                                         tGGATTACCTCCTTACCTTAAAGAAGCGTTCTTTGAAGTGCTCACACAGATTGTCTGATGAAAATGAGCAGTAAAACCTCTACAGGCTTGTAg    <  1:1390835/93‑1 (MQ=37)
                                                         tGGATTACCTCCTTACCTTAAAGAAGCGTTCTTTGAAGTGCTCACACAGATTGTCTGATGAAAATGAGCAGTAAAACCTCTACAGGCTTGTAg    <  1:5680030/93‑1 (MQ=255)
                                                          ggATTACCTCCTTACCTTAAAGAAGCGTTCTTTGAAGTGCTCACACAGATTGTCTGATGAAAATGAGCAGTAAAACCTCTACAGGCTTGTAGc   <  1:5213009/93‑1 (MQ=255)
                                                           gATTACCTCCTTACCTTAAAGAAGCGTTCTTTGAAGTGCTCACACAGATTGTCTGATGAAAATGAGCAGTAAAACCTCTACAGGCTTGTAGCt  <  1:1605468/93‑1 (MQ=255)
                                                           gATTACCTCCTTACCTTAAAGAAGCGTTCTTTGAAGTGCTCACACAGATTGTCTGATGAAAATGAGCAGTAAAACCTCTACAGGCTTGTAGCt  >  1:3858770/1‑93 (MQ=255)
                                                           gATTACCTCCTTACCTTAAAGAAGCGTTCTTTGAAGTGCTCACACAGATTGTCTGATGAAAATGAGCAGTAAAACCTCTACAGGCTTGTAGCt  <  2:4084950/93‑1 (MQ=255)
                                                              |                                                                                         
TTGTGATTCATGACTGGGGTGAAGTCGTAACAAGGTAACCGTAGGGGAACCTGCGGTTGGATCACCTCCTTACCTTAAAGAAGCGTTCTTTGAAGTGCTCACACAGATTGTCTGATGAAAATGAGCAGTAAAACCTCTACAGGCTTGTAGCT  >  NC_000913/4034590‑4034741

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
AGCTTAACCTTCGGGAGGGCGCTTACCACTTTGTGATTCATGACTGGGGTGAAGTCGTAACAAGGTAACCGTAGGGGAACCTGCGGTTGGATCACCTCCTTACCTTAAAGAAGCGTTCTTTGAAGTGCTCACACAGATTGTCTGATGAAAATGAGCAGTAAAACCTCTACAGGCTTGTAGCTCAGG  >  NC_000913/4034560‑4034745
                                                                                            |                                                                                             
AGCTTAACCTTCGGGAGGGCGCTTACCACTTTGTGATTCATGACTGGGGTGAAGTCGTAACAAGGTAACCGTAGGGGAACCTGCGGTTGGATCA                                                                                              <  SRR4302537.5877569/94‑1 (MQ=0)
AGCTTAACCTTCGGGAGGGCGCTTACCACTTTGTGATTCATGACTGGGGTGAAGTCGTAACAAGGTAACCGTAGGGGAACCTGCGGTTGGATCA                                                                                              <  SRR4302537.2505099/94‑1 (MQ=0)
 GCTTAACCTTCGGGAGGGCGCTTACCACTTTGTGATTCATGACTGGGGTGAAGTCGTAACAAGGTAACCGTAGGGGAACCTGCGGTTGGATCAC                                                                                             >  SRR4302537.2072965/1‑94 (MQ=0)
 GCTTAACCTTCGGGAGGGCGCTTACCACTTTGTGATTCATGACTGGGGTGAAGTCGTAACAAGGTAACCGTAGGGGAACCTGCGGTTGGATCAC                                                                                             >  SRR4302537.182018/1‑94 (MQ=0)
  CTTAACCTTCGGGAGGGCGCTTACCACTTTGTGATTCATGACTGGGGTGAAGTCGTAACAAGGTAACCGTAGGGGAACCTGCGGTTGGATCACC                                                                                            >  SRR4302537.5056768/1‑94 (MQ=0)
  CTTAACCTTCGGGAGGGCGCTTACCACTTTGTGATTCATGACTGGGGTGAAGTCGTAACAAGGTAACCGTAGGGGAACCTGCGGTTGGATCACC                                                                                            <  SRR4302537.3355348/94‑1 (MQ=0)
    TAACCTTCGGGAGGGCGCTTACCACTTTGTGATTCATGACTGGGGTGAAGTCGTAACAAGGTAACCGTAGGGGAACCTGCGGTTGGATCACCTC                                                                                          >  SRR4302537.1254081/1‑94 (MQ=0)
     AACCTTCGGGAGGGCGCTTACCACTTTGTGATTCATGACTGGGGTGAAGTCGTAACAAGGTAACCGTAGGGGAACCTGCGGTTGGATCACCTCC                                                                                         >  SRR4302537.718498/1‑94 (MQ=0)
     AACCTTCGGGAGGGCGCTTACCACTTTGTGATTCATGACTGGGGTGAAGTCGTAACAAGGTAACCGTAGGGGAACCTGCGGTTGGATCACCTCC                                                                                         <  SRR4302537.219353/94‑1 (MQ=0)
     AACCTTCGGGAGGGCGCTTACCACTTTGTGATTCATGACTGGGGTGAAGTCGTAACAAGGTAACCGTAGGGGAACCTGCGGTTGGATCACCTCC                                                                                         >  SRR4302537.1654599/1‑94 (MQ=0)
       CCTTCGGGAGGGCGCTTACCACTTTGTGATTCATGACTGGGGTGAAGTCGTAACAAGGTAACCGTAGGGGAACCTGCGGTTGGATTACCTCCTT                                                                                       <  SRR4302537.3932472/94‑1 (MQ=0)
       CCTTCGGGAGGGCGCTTACCACTTTGTGATTCATGACTGGGGTGAAGTCGTAACAAGGTAACCGTAGGGGAACCTGCGGTTGGATCCCCTCCTT                                                                                       <  SRR4302537.4432005/94‑1 (MQ=0)
       CCTTCGGGAGGGCGCTTACCACTTTGTGATTCATGACTGGGGTGAAGTCGTAACAAGGTAACCGTAGGGGAACCTGCGGTTGGATCACCTCCTT                                                                                       <  SRR4302537.248976/94‑1 (MQ=0)
       CCTTCGGGAGGGCGCTTACCACTTTGTGATTCATGACTGGGGTGAAGTCGTAACAAGGTAACCGTAGGGGAACCTGCGGTTGGATCACCTCCTT                                                                                       >  SRR4302537.271660/1‑94 (MQ=0)
       CCTTCGGGAGGGCGCTTACCACTTTGTGATTCATGACTGGGGTGAAGTAGTAACAAGGTAACCGTAGGGGAACCTGCGGTTGGATCACCTCCTT                                                                                       >  SRR4302537.4721969/1‑94 (MQ=0)
        CTTCGGGAGGGCGCTTACCACTTTGTGATTCATGACTGGGGTGAAGTCGTAACAAGGTAACCGTAGGGGAACCTGCGGTTGGATCACCTCCTTA                                                                                      >  SRR4302537.4234067/1‑94 (MQ=0)
        CTTCGGGAGGGCGCTTACCACTTTGTGATTCATGACTGGGGTGAAGTCGTAACAAGGTAACCGTAGGGGAACCTGCGGTTGGATCACCTCCTTA                                                                                      <  SRR4302537.1090695/94‑1 (MQ=0)
        CTTCGGGAGGGCGCTTACCACTTTGTGATTCATGACTGGGGTGAAGTCGTAACAAGGTAACCGTAGGGGAACCTGCGGTTGGATCACCTCCTTA                                                                                      >  SRR4302537.5668764/1‑94 (MQ=0)
        CTTCGGGAGGGCGCTTACCACTTTGTGATTCATGACTGGGGTGAAGTCGTAACAAGGTAACCGTAGGGGAACCTGCGGTTGGATCACCTCCTTA                                                                                      >  SRR4302537.4222486/1‑94 (MQ=0)
         TTCGGGAGGGCGCTTACCACTTTGTGATTCATGACTGGGGTGAAGTCGTAACAAGGTAACCGTAGGGGAACCTGCGGTTGGATTACCTCCTTAC                                                                                     >  SRR4302537.229075/1‑94 (MQ=0)
          TCGGGAGGGCGCTTACCACTTTGTGATTCATGACTGGGGTGAAGTCGTAACAAGGTAACCGTAGGGGAACCTGCGGTTGGATCACCTCCTTACC                                                                                    <  SRR4302537.1653524/94‑1 (MQ=0)
           CGGGAGGGCGCTTACCACTTTGTGATTCATGACTGGGGTGAAGTCGTAACAAGGTAACCGTAGGGGAACCTGCGGTTGGATCACCTCCTTACCT                                                                                   <  SRR4302537.2699040/94‑1 (MQ=0)
            GGGAGGGCGCTTACCACTTTGTGATTCATGACTGGGGTGAAGTCGTAACAAGGTAACCGTAGGGGAACCTGCGGTTGGATCAGCTCCTTACCTT                                                                                  >  SRR4302537.3156321/1‑94 (MQ=0)
            GGGAGGGCGCTTACCACTTTGTGATTCATGACTGGGGTGAAGTCGTAACAAGGTAACCGTAGGGGAACCTGCGGTTGGATCACCTCCTTACCTT                                                                                  >  SRR4302537.276225/1‑94 (MQ=0)
              GAGGGCGCTTACCACTTTGTGATTCATGACTGGGGTGAAGTCGTAACAAGGTAAGCGTAGGGGAACCTGCGGTTGGATCACCTCCTTACCTTAA                                                                                >  SRR4302537.335720/1‑94 (MQ=0)
              GAGGGCGCTTACCACTTTGTGATTCATGACTGGGGTGAAGTCGTAACAAGGTAACCGTAGGGGAACCTGCGGTTGGATCACCTCCTTACCTTAA                                                                                >  SRR4302537.1899224/1‑94 (MQ=0)
               AGTGCGCTTACCACTCTGTGATTCATGACTGGGGTGAAGTCGTACCAAGGTAACCGTAGGGGAACCTGCGGTTGGATCACCTCCTTACCTTAAA                                                                               <  SRR4302537.4077400/94‑1 (MQ=0)
                GGGCGCTTACCACTTTGTGATTCATGACTGGGGTGAAGTCGTAACAAGGTAACCGTAGGGGAACCTGCGGTTGGATCACCTCCTTACCTTAAAG                                                                              <  SRR4302537.4117894/94‑1 (MQ=0)
                GGGCGCTTACCACTTTGTGATTCATGACTGGGGTGAAGTCGTAACAAGGTAACCGTAGGGGAACCTGCGGTTGGATCACCTCCTTACCTTAAAG                                                                              <  SRR4302537.650602/94‑1 (MQ=0)
                 GGCGCTTACCACTCTGTGATTCATGACTGGGGTGAAGTCGTAACAAGGTAACCGTAGGGGAACCTGCGGTTGGATCACCTCCTTACCTTAAAGA                                                                             <  SRR4302537.3715204/94‑1 (MQ=0)
                  GCGCTTACCACTTTGTGATTCATGACTGGGGTGAAGTCGTAACAAGGTAACCGTAGGGGAACCTGCGGTTGGATCACCTCCTTACCTTAAAGAA                                                                            <  SRR4302537.9265/94‑1 (MQ=0)
                  GCGCTTACCACTTTGTGATTCATGACTGGGGTGAAGTCGTAACAAGGTAACCGTAGGGGAACCTGCGGTTGGATCACCTCCTTACCTTAAAGAA                                                                            >  SRR4302537.5163536/1‑94 (MQ=0)
                  GCGCTTACCACTTTGTGATTCATGACTGGGGTGAAGTCGTAACAAGGTAACCGTAGGGGAACCTGCGGTTGGATCACCTCCTTACCTTAAAGAA                                                                            <  SRR4302537.5475229/94‑1 (MQ=0)
                  GCGCTTACCACTTTGTGATTCATGACTGGGGTGAAGTCGTAACAAGGTAACCGTAGGGGAACCTGCGGTTGGATCACCTCCTTACCTTAAAGAA                                                                            <  SRR4302537.4187445/94‑1 (MQ=0)
                   CGCTTACCACTTTGTGATTCATGACTGGGGTGAAGTCGTAACAAGGTAACCGTAGGGGAACCTGCGGTTGGATTACCTCCTTACCTTAAAGAAG                                                                           >  SRR4302537.5725871/1‑94 (MQ=0)
                   CGCTTACCACTTTGTGATTCATGACTGGGGTGAAGTCGTAACAAGGTAACCGTAGGGGAACCTGCGGTTGGATCACCTCCTTACCTTAAAGAAG                                                                           <  SRR4302537.4641321/94‑1 (MQ=0)
                    GCTTACCACTTTGTGATTCATGACTGGGGTGAAGTCGTAACAAGGTAACCGTAGGGGAACCTGCGGTTGGATCACCTCCTTACCTTAAAGAAGC                                                                          <  SRR4302537.6438772/94‑1 (MQ=0)
                      TTACCACTTTGTGATTCATGACTGGGGTGAAGTCGTAACAAGGTAACCGTAGGGGAACCTGCGGTTGGATCACCTCCTTACCTTAAAGAAGCGT                                                                        <  SRR4302537.1269961/94‑1 (MQ=0)
                      TTACCACTTTGTGATTCATGACTGGGGTGAAGTCGTAACAAGGTAACCGTAGGGGAACCTGCGGTTGGATCACCTCCTTACCTTAAAGAAGCGT                                                                        >  SRR4302537.5165185/1‑94 (MQ=0)
                       TACCACTTTGTGATTCATGACTGGGGTGAAGTCGTAACAAGGTAACCGTAGGGGAACCTGCGGTTGGATCACCTCCTTACCTTAAAGAAGCGTT                                                                       <  SRR4302537.1805153/94‑1 (MQ=0)
                       TACCACTTTGTGATTCATGACTGGGGTGAAGTCGTAACAAGGTAACCGTAGGGGAACCTGCGGTTGGATCACCTCCTTACCTTAAAGAAGCGTT                                                                       <  SRR4302537.1078076/94‑1 (MQ=0)
                       TACCACTTTGTGATTCATGACTGGGGTGAAGTCGTAACAAGGTAACCGTAGGGGAACCTGCGGTTGGATCACCTCCTTACCTTAAAGAAGCGTT                                                                       <  SRR4302537.5404030/94‑1 (MQ=0)
                       TACCACTTTGTGATTCATGACTGGGGTGAAGTCGTAACAAGGTAACCGTAGGGGAACCTGCGGTTGGATCACCTCCTTACCTTAAAGAAGCGTT                                                                       <  SRR4302537.3741500/94‑1 (MQ=0)
                       TACCACTTTGTGATTCATGACTGGGGTGAAGTCGTAACAAGGTAACCGTAGGGGAACCTGCGGTTGGATCACCTCCTTACCTTAAAGAAGCGTT                                                                       >  SRR4302537.1985914/1‑94 (MQ=0)
                        ACCACTTTGTGATTCATGACTGGGGTGAAGTCGTAACAAGGTAACCGTAGGGGAACCTGCGGTTGGATCACCTCCTTACCTTAAAGAAGCGTTC                                                                      <  SRR4302537.5555632/94‑1 (MQ=0)
                         CCACTTTGTGATTCATGACTGGGGTGAAGTCGTAACAAGGTAACCGTAGGGGAACCTGCGGTTGGATCACCTCCTTACCTTAAAGAAGCGTTCT                                                                     <  SRR4302537.4178311/94‑1 (MQ=0)
                         CCACTTTGTGATTCATGACTGGGGGGAAGTCGTAACAAGGTAACCGTAGGGGAACCTGCGGTTGGATCACCGCCTTACCTTAAAGAtgtgtact                                                                     >  SRR4302537.4351387/1‑86 (MQ=0)
                           CCTTTGTGATTCATGACTGGGGTGAAGTCGTAACAAGGTAACCGTAGGGGAACCTGCGGTTGGATTACCTCCTTACCTTAAAGAAGCGTTCTTT                                                                   <  SRR4302537.1238753/94‑1 (MQ=0)
                           ACTTTGTGATTCATGACTGGGGTGAAGTCGTAACAAGGTAACCGTAGGGGAACCTGCGGTTGGATCACCTCCTTACCTTAAAGAAGCGTTCTTT                                                                   <  SRR4302537.302118/94‑1 (MQ=0)
                            CTTTGTGATTCATGACTGGGGTGAAGTCGTAACAAGGTAACCGTAGGGGAACCTGCGGTTGGATTACCTCCTTACCTTAAAGAAGCGTTCTTTG                                                                  >  SRR4302537.3330475/1‑94 (MQ=0)
                            CTTTGTGATTCATGACTGGGGTGAAGTCGTAACAAGGTAACCGTAGGGGAACCTGCGGTTGGATTACCTCCTTACCTTAAAGAAGCGTTCTTTG                                                                  <  SRR4302537.2252074/94‑1 (MQ=0)
                            CTTTGTGATTCATGACTGGGGTGAAGTCGTAACAAGGTAACCGTAGGGGAACCTGCGGTTGGATTACCTCCTTACCTTAAAGAAGCGTTCTTTG                                                                  >  SRR4302537.5672595/1‑94 (MQ=0)
                             TTTGTGATTCATGACTGGGGTGAAGTCGTAACAAGGTAACCGTAGGGGAACCTGCGGTTGGATTACCTCCTTACCTTAAAGAAGCGTTCTTTGA                                                                 >  SRR4302537.3294508/1‑94 (MQ=0)
                              TTGTGATTCATGACTGGGGTGAAGTCGTAACAAGGTAACCGTAGGGGAACCTGCGGTTGGATTACCTCCTTACCTTAAAGAAGCGTTCTTTGAA                                                                <  SRR4302537.4051666/94‑1 (MQ=0)
                                GTGATTCATGACTGGGGTGAAGTCGTAACAAGGTAACCGTAGGGGAACCTGCGGTTGGATTACCTCCTTACCTTAAAGAAGCGTTCTTTGAAGT                                                              <  SRR4302537.3934724/94‑1 (MQ=5)
                                GTGATTCATGACTGGGGTGAAGTCGTAACAAGGTAACCGTAGGGGAACCTGCGGTTGGATTACCTCCTTACCTTAAAGAAGCGTTCTTTGAAGT                                                              <  SRR4302537.1236199/94‑1 (MQ=5)
                                 TGATTCATGACTGGGGTGAAGTCGTAACAAGGTAACCGTAGGGGAACCTGCGGTTGGATTACCTCCTTACCTTAAAGAAGCGTTCTTTGAAGTG                                                             >  SRR4302537.3791911/1‑94 (MQ=8)
                                 TGATTCATGACTGGGGTGAAGTCGTAACAAGGTAACCGTAGGGGAACCTGCGGTTGGATTACCTCCTTACCTTAAAGAAGCGTTCTTTGAAGTG                                                             >  SRR4302537.3668395/1‑94 (MQ=8)
                                 TGATTCATGACTGGGGTGAAGTCGTAACAAGGTAACCGTAGGGGAACCTGCGGTTGGATTACCTCCTTACCTTAAAGAAGCGTTCTTTGAAGTG                                                             >  SRR4302537.1389246/1‑94 (MQ=8)
                                 TGATTCATGACTGGGGTGAAGTCGTAACAAGGTAACCGTAGGGGAACCTGCGGTTGGATTACCTCCTTACCTTAAAGAAGCGTTCTTTGAAGTG                                                             <  SRR4302537.4037948/94‑1 (MQ=8)
                                  GATTCATGACTGGGGTGAAGTCGTAACAAGGTAACCGTAGGGGAACCTGCGGTTGGATTACCTCCTTACCTTAAAGAAGCGTTCTTTGAAGTGC                                                            >  SRR4302537.4054754/1‑94 (MQ=8)
                                   ATTCATGACTGGGGTGAAGTCGTAACAAGGTAACCGTAGGGGAACCTGCGGTTGGATTACCTCCTTACCTTAAAGAAGCGTTCTTTGAAGTGCT                                                           >  SRR4302537.118371/1‑94 (MQ=8)
                                    TTCATGACTGGGGTGAAGTCGTAACAAGGTAACCGTAGGGGAACCTGCGGTTGGATTACCTCCTTACCTTAAAGAAGCGTTCTTTGAAGTGCTC                                                          <  SRR4302537.1885801/94‑1 (MQ=8)
                                     TCATGACTGGGGTGAAGTCGTAACAAGGTAACCGTAGGGGAACCTGCGGTTGGATTACCTCCTTACCTTAAAGAAGCGTTCTTTGAAGTGCTCA                                                         <  SRR4302537.736541/94‑1 (MQ=8)
                                     TCATGACTGGGGTGAAGTCGTAACAAGGTAACCGTAGGGGAACCTGCGGTTGGATTACCTCCTTACCTTAAAGAAGCGTTCTTTGAAGTGCTCA                                                         <  SRR4302537.73908/94‑1 (MQ=8)
                                      CATGACTGGGGTGAAGTCGTAACAAGGTAACCGTAGGGGAACCTGCGGTTGGATTACCTCCTTACCTTAAAGAAGCGTTCTTTGAAGTGCTCAC                                                        <  SRR4302537.6297449/94‑1 (MQ=8)
                                      CATGACTGGGGTGAAGTCGTAACAAGGTAACCGTAGGGGAACCTGCGGTTGGATTACCTCCTTACCTTAAAGAAGCGTTCTTTGAAGTGCTCAC                                                        >  SRR4302537.2737124/1‑94 (MQ=8)
                                       ATGACTGGGGTGAAGTCGTAACAAGGTAACCGTAGGGGAACCTGCGGTTGGATTACCTCCTTACCTTAAAGAAGCGTTCTTTGAAGTGCTCACA                                                       <  SRR4302537.729394/94‑1 (MQ=8)
                                        TGACTGGGGTGAAGTCGTAACAAGGTAACCGTAGGGGAACCTGCGGTTGGATTACCTCCTTACCTTAAAGAAGCGTTCTTTGAAGTGCTCACAC                                                      <  SRR4302537.4561057/94‑1 (MQ=8)
                                         GACTGGGGTGAAGTCGTAACAAGGTAACCGTAGGGGAACCTGCGGTTGGATTACCTCCTTACCTTAAAGAAGCGTTCTTTGAAGTGCTCACACA                                                     <  SRR4302537.3187148/94‑1 (MQ=8)
                                         GACTGGGGTGAAGTCGTAACAAGGTAACCGTAGGGGAACCTGCGGTTGGATTACCTCCTTACCTTAAAGAAGCGTTCTTTGAAGTGCTCACACA                                                     >  SRR4302537.2155664/1‑94 (MQ=8)
                                          ACTGGGGTGAAGTCGTAACAAGGTAACCGTAGGGGAACCTGCGGTTGGATTACCTCCTTACCTTAAAGAAGCGTTCTTTGAAGTGCTCACACAG                                                    <  SRR4302537.4931730/94‑1 (MQ=8)
                                          ACTGGGGTGAAGTCGTAACAAGGTAACCGTAGGGGAACCTGCGGTTGGATTACCTCCTTACCTTAAAGAAGCGTTCTTTGAAGTGCTCACACAG                                                    <  SRR4302537.4822966/94‑1 (MQ=8)
                                            TGGGGTGAAGTCGTAACAAGGTAACCGTAGGGGAACCTGCGGTTGGATTACCTCCTTACCTTAAAGAAGCGTTCTTTGAAGTGCTCACACAGAT                                                  >  SRR4302537.4994903/1‑94 (MQ=8)
                                            TGGGGTGAAGTCGTAACAAGGTAACCGTAGGGGAACCTGCGGTTGGATTACCTCCTTACCTTAAAGAAGCGTTCTTTGAAGTGCTCACACAGAT                                                  <  SRR4302537.1239462/94‑1 (MQ=8)
                                                GTGAAGTCGTAACAAGGTAACCGTAGGGGAACCTGCGGTTGGATTACCTCCTTACCTTAAAGAAGCGTTCTTTGAAGTGCTCACAGAGATTGTC                                              >  SRR4302537.3563952/1‑94 (MQ=7)
                                                 TGAAGTCGTAACAAGGTAACCGTAGGGGAACCTGCGGTTGGATTACCTCCTTACCTTAAAGAAGCGTTCTTTGAAGTGCTCACACAGATTGTCT                                             <  SRR4302537.4089619/94‑1 (MQ=8)
                                                 TGAAGTCGTAACAAGGTAACCGTAGGGGAACCTGCGGTTGGATTACCTCCTTACCTTAAAGAAGCGTTCTTTGAAGTGCTCACACAGATTGTCT                                             <  SRR4302537.5066723/94‑1 (MQ=8)
                                                 TGAAGTCGTAACAAGGTAACCGTAGGGGAACCTGCGGTTGGATTACCTCCTTACCTTAAAGAAGCGTTCTTTGAAGTGCTCACACAGATTGTCT                                             >  SRR4302537.2040161/1‑94 (MQ=8)
                                                 TGAAGTCGTAACAAGGTAACCGTAGGGGAACCTGCGGTTGGATTACCTCCTTACCTTAAAGAAGCGTTCTTTGAAGTGCTCACACAGATTGTCT                                             >  SRR4302537.296193/1‑94 (MQ=8)
                                                 TGAAGTCGTAACAAGGTAACCGTAGGCGAACCTGCGGTTGGATTACCTCCTTACCTTAAAGAAGCGTTCTTTGAAGTGCTCACACAGATTGTCT                                             <  SRR4302537.4224468/94‑1 (MQ=7)
                                                  GAAGTCGTAACAAGGTAACCGTAGGGGAACCTGCGGTTGGATTACCTCCTTACCTTAAAGAAGCGTTCTTTGAAGTGCTCACACAGATTGTCTG                                            <  SRR4302537.4637003/94‑1 (MQ=8)
                                                   AAGTCGCAACAAGGTAACCGTAGGGGAACCTGCGGTTGGATTACCTCCTTACCTTAAAGAAGCGTTCTTTGAAGTGCTCACACAGATTGTCTGA                                           <  SRR4302537.2298824/94‑1 (MQ=7)
                                                    AGTCGTAACAAGGTAACCGTAGGGGAACCTGCGGTTGGATTACCTCCTTACCTTAAAGAAGCGTTCTTTGAAGTGCTCACACAGATTGTCTGAT                                          <  SRR4302537.5149582/94‑1 (MQ=8)
                                                    AGTCGTAACAAGGTAACCGTAGGGGAACCTGCGGTTGGATTACCTCCTTACCTTAAAGAAGCGTTCTTTGAAGTGCTCACACAGATTGTCTGAT                                          <  SRR4302537.4484459/94‑1 (MQ=8)
                                                     GTCGTAACAAGGTAACCGTAGGGGAACCTGCGGTTGGATTACCTCCTTACCTTAAAGAAGCGTTCTTTGAAGTGCTCACACAGATTGTCTGATG                                         <  SRR4302537.1567767/94‑1 (MQ=10)
                                                     GTCGTAACAAGGTAACCGTAGGGGAACCTGCGGTTGGATTACCTCCTTACCTTAAAGAAGCGTTCTTTGAAGTGCTCACACAGATTGTCTGATG                                         <  SRR4302537.5621099/94‑1 (MQ=10)
                                                     GTCGTAACAAGGTAACCGTAGGGGAACCTGCGGTTGGATTACCTCCTTACCTTAAAGAAGCGTTCTTTGAAGTGCTCACACAGATTGTCTGATG                                         <  SRR4302537.3401523/94‑1 (MQ=10)
                                                       CGTAACAAGGTAACCGTAGGGGAACCTGCGGTTGGATTACCTCCTTACCTTAAAGAAGCGTTCTTTGAAGTGCTCACACAGATTGTCTGATGAA                                       >  SRR4302537.1794592/1‑94 (MQ=20)
                                                          AACAAGGTAACCGTAGGGGAACCTGCGGTTGGATTACCTCCTTACCTTAAAGAAGCGTTCTTTGAAGTGCTCACACAGATTGTCTGATGAAAAT                                    >  SRR4302537.6228291/1‑94 (MQ=28)
                                                          AACAAGGTAACCGTAGGGGAACCTGCGGTTGGATTACCTCCTTACCTTAAAGAAGCGTTCTTTGAAGTGCTCACACAGATTGTCTGATGAAAAT                                    <  SRR4302537.6269629/94‑1 (MQ=25)
                                                          AACAAGGTAACCGTAGGGGAACCTGCGGTTGGATTACCTCCTTACCTTAAAGAAGCGTTCTTTGAAGTGCTCACACAGATTGTCTGATGAAAAT                                    >  SRR4302537.137746/1‑94 (MQ=28)
                                                            CAAGGTAACCGTAGGGGAACCTGCGGTTGGATTACCTCCTTACCTTAAAGAAGCGTTCTTTGAAGTGCTCACACAGATTGTCTGATGAAAATGA                                  <  SRR4302537.550067/94‑1 (MQ=25)
                                                             AAGGTAACCGTAGGGGAACCTGCGGTTGGATTACCTCCTTACCTTAAAGAAGCGTTCTTTGAAGTGCTCACACAGATTGTCTGATGAAAATGAG                                 <  SRR4302537.2482111/94‑1 (MQ=25)
                                                              AGGTAACCGTAGGGGAACCTGCGGTTGGATTACCTCCTTACCTTAAAGAAGCGTTCTTTGAAGTGCTCACACAGATTGTCTGATGAAAATGAGC                                <  SRR4302537.3413994/94‑1 (MQ=25)
                                                               GGTAACCGTAGGGGAACCTGCGGTTGGATTACCTCCTTACCTTAAAGAAGCGTTCTTTGAAGTGCTCACACAGATTGTCTGATGAAAATGAGCA                               <  SRR4302537.730641/94‑1 (MQ=25)
                                                               GGTAACCGTAGGGGAACCTGCGGTTGGATTACCTCCTTACCTTAAAGAAGCGTTCTTTGAAGTGCTCACACAGATTGTCTGATGAAAATGAGCA                               <  SRR4302537.4375111/94‑1 (MQ=25)
                                                                GTAACCGTAGGGGAACCTGCGGTTGGATTACCTCCTTACCTTAAAGAAGCGTTCTTTGAAGTGCTCACACAGATTGTCTGATGAAAATGAGCAG                              <  SRR4302537.6017005/94‑1 (MQ=25)
                                                                GTAACCGTAGGGGAACCTGCGGTTGGATTACCTCCTTACCTTAAAGAAGCGTTCTTTGAAGTGCTCACACAGATTGTCTGATGAAAATGAGCAG                              <  SRR4302537.1549828/94‑1 (MQ=25)
                                                                GTAACCGTAGGGGAACCTGCGGTTGGATTACCTCCTTACCTTAAAGAAGCGTTCTTTGAAGTGCTCACACAGATTGTCTGATGAAAATGAGCAG                              <  SRR4302537.5668651/94‑1 (MQ=25)
                                                                  AACCGTAGGGGAACCTGCGGTTGGATTACCTCCTTACCTTAAAGAAGCGTTCTTTGAAGTGCTCACACAGATTGTCTGATGAAAATGAGCAGTA                            >  SRR4302537.3840276/1‑94 (MQ=25)
                                                                  AACCGTAGGGGAACCTGCGGTTGGATTACCTCCTTACCTTAAAGAAGCGTTCTTTGAAGTGCTCACACAGATTGTCTGATGAAAATGAGCAGTA                            <  SRR4302537.1138272/94‑1 (MQ=25)
                                                                  AACCGTAGGGGAACCTGCGGTTGGATTACCTCCTTACCTTAAAGAAGCGTGCTTTGAAGTGCTCACACAGATTGTCTGATGAAAATGAGCAGTA                            <  SRR4302537.3483216/94‑1 (MQ=7)
                                                                   ACCGTAGGGGAACCTGCGGTTGGATTACCTCCTTACCTTAAAGGAGCGTTCTTTGAAGTGTTCTCACAGCTTGTCTGATGAAAATGAGCAGTAA                           >  SRR4302537.3494850/1‑94 (MQ=21)
                                                                   ACCGTAGGGGAACCTGCGGTTGGATTACCTCCTTACCTTAAAGAAGCGTTCTTTGAAGTGCTCACACAGATTGTCTGATGAAAATGAGCAGTAA                           <  SRR4302537.372371/94‑1 (MQ=25)
                                                                   ACCGTAGGGGAACCTGCGGTTGGATTACCTCCTTACCTTAAAGAAGCGTTCTTTGAAGTGCTCACACAGATTGTCTGATGAAAATGAGCAGTAA                           >  SRR4302537.3234200/1‑94 (MQ=25)
                                                                   ACCGTAGGGGAACCTGCGGTTGGATTACCTCCTTACCTTAAAGAAGCGTTCTTTGAAGTGCTCACACAGATTGTCTGATGAAAATGAGCAGTAA                           >  SRR4302537.758862/1‑94 (MQ=25)
                                                                    CCGTAGGGGAACCTGCGGTTGGATTACCTCCTTACCTTAAAGAAGCGTTCTTTGAAGTGCTCACACAGATTGTCTGATGAAAATGAGCAGTAAA                          >  SRR4302537.4330774/1‑94 (MQ=25)
                                                                     CGTAGGGGAACCTGCGGTTGGATTACCTCCTTACCTTAAAGAAGCGTTCTTTGAAGTGCTCACACAGATTGTCTGATGAAAATGAGCAGTAAAA                         >  SRR4302537.170221/1‑94 (MQ=25)
                                                                     CGTAGGGGAACCTGCGGTTGGATTACCTCCTTACCTTAAAGAAGCGTTCTTTGAAGTGCTCACACAGATTGTCTGATGAAAATGAGCAGTAAAA                         >  SRR4302537.1497568/1‑94 (MQ=25)
                                                                     CGTAGGGGAACCTGCGGTTGGATTACCTACTTACCTTAAAGAAGCGTTCTTTGAAGTGCTCACACAGATTGTCTGATGAAAATGAGCAGTAAAA                         >  SRR4302537.6247883/1‑94 (MQ=24)
                                                                      GTAGGGGAACCTGCGGTTGGATTACCTCCTTACCTTAAAGAAGCGTTCTTTGAAGTGCTCACACAGATTGTCTGATGAAAATGAGCAGTAAAAC                        <  SRR4302537.2479156/94‑1 (MQ=25)
                                                                       TAGGGGAACCTGCGGTTGGATTACCTCCTTACCTTAAAGAAGCGTTCTTTGAAGTGCTCACACAGATTGTCTGATGAAAATGAGCAGTAAAACC                       <  SRR4302537.3762217/94‑1 (MQ=25)
                                                                       TAGGGGAACCTGCGGTTGGATTACCTCCTTACCTTAAAGAAGCGTTCTTTGAAGTGCTCACACAGATTGTCTGATGAAAATGAGCAGTAAAACC                       <  SRR4302537.6126009/94‑1 (MQ=25)
                                                                        AGGGGAACCTGCGGTTGGATTACCTCCTTACCTTAAAGAAGCGTTCTTTGAAGTGCTCACACAGATTGTCTGATGAAAATGAGCAGTAAAACCT                      <  SRR4302537.5392900/94‑1 (MQ=25)
                                                                         GGGGAACCTGCGGTTGGATTACCTCCTTACCTTAAAGAAGCGTTCTTTGAAGTGCTCACACAGATTGTCTGATGAAAATGAGCAGTAAAACCTC                     >  SRR4302537.1596332/1‑94 (MQ=25)
                                                                          GGGAACCTGCGGTTGGATTACCTCCTTACCTTAAAGAAGCGTTCTTTGAAGTGCTCACACAGATTGTCTGATGAAAATGAGCAGTAAAACCTCT                    <  SRR4302537.333751/94‑1 (MQ=25)
                                                                          GGGAACCTGCGGTTGGATTACCTCCTTACCTTAAAGAAGCGTTCTTTGAAGTGCTCACACAGATTGTCTGATGAAAATGAGCAGTAAAACCTCT                    <  SRR4302537.5113178/94‑1 (MQ=25)
                                                                            GAACCTGCGGTTGGATTACCTCCTTACCTTAAAGAAGCGTTCTTTGAAGTGCTCACACAGATTGTCTGATGAAAATGAGCAGTAAAACCTCTAC                  <  SRR4302537.61567/94‑1 (MQ=25)
                                                                             AACCTGCGGTTGGATTACCTCCTTACCTTAAAGAAGCGTTCTTTGAAGTGCTCACACAGATTGTCTGATGAAAATGAGCAGTAAAACCTCTACA                 <  SRR4302537.6128658/94‑1 (MQ=25)
                                                                             AACCTGCGGTTGGATTACCTCCTTACCTTAAAGAAGCGTTCTTTGAAGTGCTCACACAGATTGTCTGATGAAAATGAGCAGTAAAACCTCTACA                 >  SRR4302537.1593336/1‑94 (MQ=25)
                                                                              ACCTGCGGTTGGATTACCTCCTTACCTTAAAGAAGCGTTCTTTGAAGTGCTCACACAGATTGTCTGATGAAAATGAGCAGTAAAACCTCTACAG                <  SRR4302537.2711431/94‑1 (MQ=25)
                                                                              ACCTGCGGTTGGATTACCTCCTTACCTTAAAGAAGCGTTCTTTGAAGTGCTCACACAGATTGTCTGATGAAAATGAGCAGTAAAACCTCTACAG                <  SRR4302537.2025478/94‑1 (MQ=25)
                                                                                CTGCGGTTGGATTACCTCCTTACCTTAAAGAAGCGTTCTTTGAAGTGCTCACACAGATTGTCTGATGAAAATGAGCAGTAAAACCTCTACAGGC              >  SRR4302537.3236362/1‑94 (MQ=25)
                                                                                 TGCGGTTGGATTACCTCCTTACCTTAAAGAAGCGTTCTGTGAAGTGCTCACACAGATTGTCTGATGAAAATGAGCAGTAAAACCTCTACAGGCT             <  SRR4302537.1856488/94‑1 (MQ=24)
                                                                                  GCGGTTGGATTACCTCCTTACCTTAAAGAAGCGTTCTTTGAAGTGCTCACACAGATTGTCTGATGAAAATGAGCAGTAAAACCTCTACAGGCTT            <  SRR4302537.1505167/94‑1 (MQ=25)
                                                                                  GCGGTTGGATTACCTCCTTACCTTAAAGAAGCGTTCTTTGAAGTGCTCACACAGATTGTCTGATGAAAATGAGCAGTAAAACCTCTACAGGCTT            >  SRR4302537.3318933/1‑94 (MQ=25)
                                                                                  GCGGTTGGATTACCTCCTTACCTTAAAGAAGCGTTCTTTGAAGTGCTCACACAGATTGTCTGATGAAAATGAGCAGTAAAACCTCTACAGGCTT            <  SRR4302537.5068048/94‑1 (MQ=25)
                                                                                    GGTTGGATTACCTCCTTACCTTAAAGAAGCGTTCTTTGAAGTGCTCACACAGATTGTCTGATGAAAATGAGCAGTAAAACCTCTACAGGCTTGT          >  SRR4302537.4119577/1‑94 (MQ=25)
                                                                                    GGTTGGATTACCTCCTTACCTTAAAGAAGCGTTCTTTGAAGTGCTCACACAGATTGTCTGATGAAAATGAGCAGTAAAACCTCTACAGGCTTGT          >  SRR4302537.1614362/1‑94 (MQ=25)
                                                                                    GGTTGGATTACCTCCTTACCTTAAAGAAGCGTTCTTTGAAGTGCTCACACAGATTGTCTGATGAAAATGAGCAGTAAAACCTCTACAGGCTTGT          >  SRR4302537.2499971/1‑94 (MQ=25)
                                                                                     GTTGGATTACCTCCTTACCTTAAAGAAGCGTTCTTTGAAGTGCTCACACAGATTGTCTGATGAAAATGAGCAGTAAAACCTCTACAGGCTTGTA         <  SRR4302537.5616509/94‑1 (MQ=25)
                                                                                     GTTGGATTACCTCCTTACCTTAAAGAAGCGTGCTTTGAAGTGCTCACACAGATTGTCTGATGAAAATGAGCAGTAAAACCTCTACAGGCTTGTA         <  SRR4302537.5457121/94‑1 (MQ=7)
                                                                                      TTGGATTACCTCCTTACCTTAAAGAAGCGTTCTTTGAAGTGCTCACACAGATTGTCTGATGAAAATGAGCAGTAAAACCTCTACAGGCTTGTAG        <  SRR4302537.2365336/94‑1 (MQ=25)
                                                                                       TGGATTACCTCCTTACCTTAAAGAAGCGTTCTTTGAAGTGCTCACACAGATTGTCTGATGAAAATGAGCAGTAAAACCTCTACAGGCTTGTAGC       <  SRR4302537.1400536/94‑1 (MQ=25)
                                                                                       TGGATTACCTCCTTACCTTAAAGAAGCGTTCTTTGAAGTGCTCACACAGATTGTCTGATGAAAATGAGCAGTAAAACCTCTACAGGCTTGTAGC       <  SRR4302537.5718885/94‑1 (MQ=25)
                                                                                        GGATTACCTCCTTACCTTAAAGAAGCGTTCTTTGAAGTGCTCACACAGATTGTCTGATGAAAATGAGCAGTAAAACCTCTACAGGCTTGTAGCT      >  SRR4302537.6357153/1‑94 (MQ=25)
                                                                                        GGATTACCTCCTTACCTTAAAGAAGCGTTCTTTGAAGTGCTCACACAGATTGTCTGATGAAAATGAGCAGTAAAACCTCTACAGGCTTGTAGCT      <  SRR4302537.5248852/94‑1 (MQ=25)
                                                                                        GGATTACCTCCTTACCTTAAAGAAGCGTTCTTTGAAGTGCTCACACAGATTGTCTGATGAAAATGAGCAGTAAAACCTCTACAGGCTTGTAGCT      >  SRR4302537.3885784/1‑94 (MQ=25)
                                                                                         GATTACCTCCTTACCTTAAAGAAGCGTTCTTTGAAGTGCTCACACAGATTGTCTGATGAAAATGAGCAGTAAAACCTCTACAGGCTTGTAGCTC     <  SRR4302537.1616696/94‑1 (MQ=25)
                                                                                          ATTACCTCCTTACCTTAAAGAAGCGTTCTTTGAAGTGCTCACACAGATTGTCTGATGAAAATGAGCAGTAAAACCTCTACAGGCTTGTAGCTCA    >  SRR4302537.5191520/1‑94 (MQ=26)
                                                                                           TTACCTCCTTACCTTAAAGAAGCGTTCTTTGAAGTGCTCACACAGATTGTCTGATGAAAATGAGCAGTAAAACCTCTACAGGCTTGTAGCTCAG   <  SRR4302537.763367/94‑1 (MQ=26)
                                                                                            TACCTCCTTACCTTAAAGAAGCGTTCTTTGAAGTGCTCACACAGATTGTCTGATGAAAATGAGCAGTAAAACCTCTACAGGCTTGTAGCTCAGG  >  SRR4302537.1530577/1‑94 (MQ=26)
                                                                                            |                                                                                             
AGCTTAACCTTCGGGAGGGCGCTTACCACTTTGTGATTCATGACTGGGGTGAAGTCGTAACAAGGTAACCGTAGGGGAACCTGCGGTTGGATCACCTCCTTACCTTAAAGAAGCGTTCTTTGAAGTGCTCACACAGATTGTCTGATGAAAATGAGCAGTAAAACCTCTACAGGCTTGTAGCTCAGG  >  NC_000913/4034560‑4034745

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 18 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑