Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A1 F1 I2 R1	882	254.4	12957588	98.0%	12698436	93.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,069,524	A→G	R236R (CGT→CGC)	yihT ←	putative aldolase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,069,524	0	A	G	99.6%	817.0 / NA	239	R236R (CGT→CGC)	yihT	putative aldolase
Reads supporting (aligned to +/- strand): ref base A (0/1); new base G (94/143); total (94/145)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.99e-01

Only 100 of 249 total aligned reads displayed.

CTCGTTGTCTGGTAAACCGACGACCGATGCCCAGACTGCACGGCCTGCCAGGAATCCCGATGCGCCCGCCGTCATTGCCACGCGTACGGCACGCGGGAACAGTTTTTCGTCGACACCGGAAGAGAGGATCACCCATGGCATATTGATATGGTCATTCAGACGTTGTGAAGCACAGAGAAG  >  NC_000913/4069434‑4069613

 tCGTTGTCTGGTAAACCGACGACCGATGCCCAGACTGCACGGCCTGCCAGGAATCCCGATGCGCCCGCCGTCATTGCCACGCGTACGgcgcgc                                                                                        >  1:3284191/1‑93 (MQ=255)

  cGTTGTCTGGTAAACCGACGACCGATGCCCAGACTGCACGGCCTGCCAGGAATCCCGATGCGCCCGCCGTCATTGCCACGCGTACGGcgcgcg                                                                                       >  2:1390262/1‑93 (MQ=255)

   gTTGTCTGGTAAACCGACGACCGATGCCCAGACTGCACGGCCTGCCAGGAATCCCGATGCGCCCGCCGTCATTGCCACGCGTACGGCGCGCgg                                                                                      >  2:3290/1‑93 (MQ=255)

   gTTGTCTGGTAAACCGACGACCGATGCCCAGACTGCACGGCCTGCCAGGAATCCCGATGCGCCCGCCGTCATTGCCACGCGTACGGCGCGCgg                                                                                      <  2:5950843/93‑1 (MQ=255)

      gTCTGGTAAACCGACGACCGATGCCCAGACTGCACGGCCTGCCAGGAATCCCGATGCGCCCGCCGTCATTGCCACGCGTACGGCGCGCGGGaa                                                                                   >  2:3022248/1‑93 (MQ=255)

       tCTGGTAAACCGACGACCGATGCCCAGACTGCACGGCCTGCCAGGAATCCCGATGCGCCCGCCGTCATTGCCACGCGTACGGCGCGCGGGAAc                                                                                  >  1:3701408/1‑93 (MQ=255)

        cTGGTAAACCGACGACCGATGCCCAGACTGCACGGCCTGCCAGGAATCCGGATGCGCCCGCCGTCATTGCCACGCGTACGGCGCGCGGGAACa                                                                                 <  2:912473/93‑1 (MQ=255)

        cTGGTAAACCGACGACCGATGCCCAGACTGCACGGCCTGCCAGGAATCCCGATGCGCCCGCCGTCATTGCCACGCGTACGGCGCGCGTGAACa                                                                                 >  1:1973310/1‑93 (MQ=255)

         tGGTAAACCGACGACCGATGCCCAGACTGCACGGCCTGCCAGGAATCCCGATGCGCCCGCCGTCATTGCCACGCGTACGGCGCGCGGGAACAg                                                                                >  1:3023031/1‑93 (MQ=255)

          ggTAAACCGACGCCCGATGCCCAGACTGTACGGCCTGCCAGGAATCCCGATGCGCTAGCCGTCATTGGCCCGCGTACGGTGCGCGGGAACAgg                                                                               >  2:2817657/1‑92 (MQ=255)

            gaaaCCGACGACCGATGCCCAGACTGCACGGCCTGCCAGGAATCCCGATGCGCCCGCCGTCATTGCCACGCGTACGGCGCGCGGGAACAGttt                                                                             <  1:2032665/92‑1 (MQ=255)

               aCCGCCGACGAATGCCCAGACTGCACGGCCTGCCGGGATTCCCGAGGCGCCCGCCGTCATTGCCACGCGTACGGCGCGCGGGAACAGTTTTtc                                                                          <  1:378654/93‑1 (MQ=255)

                ccGACGACCGATGCCCAGCCTGCACGGCCTGCCAGGAATCCCGATGCGCCCGCCGTCATTGCCACGCGTACGGCGCGCGGGAACAGTTTTtcg                                                                         <  2:2565175/93‑1 (MQ=255)

                  gacgacCGATGCCCAGACTGCACGGCCTGCCAGGAATCCCGATGCGCCCGCCGTCATTGCCACGCGTACGGCGCGCGGGAACAGTTTTtcgtc                                                                       >  1:4262368/1‑93 (MQ=255)

                   acgacCGATGCCCAGACTGCACGGCCTGCCAGGAATCCCGATGCGCCCGCCGTCATTGCCACGCGTACGGCGCGCGGGAACAGCTTTtcgtag                                                                      >  2:1942992/1‑91 (MQ=255)

                     gacCGATGCCCAGACTGCACGGCCTGCCAGGAATCCCGATGCGCCCGCCGTCATTGCCACGCGTACGGCGCGCGGGAACAGTTTTtcgtcgca                                                                    >  2:6137572/1‑91 (MQ=255)

                     gacCGATGCCCAGACTGCACGGCCTGCCAGGAATCCCGATGCGCCCGCCGTCATTGCCACGCGTACGGCGCGCGGGAACAGTTTTTCGTCGac                                                                    >  2:5430644/1‑93 (MQ=255)

                     cacCGATGCCCAGACTGCACGCCCTGCCAGGAATCCCGATGCGCCCGCCGTCATTGCCACGCGTACGGCGCGCGGGAACAGTTTTTCGTCGac                                                                    <  2:3108981/92‑1 (MQ=255)

                      acCGATGCCCAGACTGCACGGCCTGCCAGGAATCCCGATGCGCCCGCCGTCATTGCCACGCGTACGGCGCGCGGGAACAGTTTTTCGTCGaca                                                                   >  2:2406833/1‑93 (MQ=255)

                       ccGATGCCCAGACTGCACGGCCTGCCAGGAATCCCGATGCGCCCGCCGTCATTGCCACGCGTACGGCGCGCGGGAACAGTTTTTCGTCGacac                                                                  <  1:1805223/93‑1 (MQ=255)

                       ccGATGCCCAGACTGCACGGCCTGCCAGGAATCCCGATGCGCCCGCCGTCATTGCCACGCGTACGGCGCGCGGGAACAGTTTTTCGTCGacac                                                                  >  2:1844922/1‑93 (MQ=255)

                        cGATGCCCAGACTGCACGGCCTGCCAGGAATCCCGATGCGCCCGCCGTCATTGCCACGCGTACGGCGCGCGGGAACAGTTTTTCGTCGACAcc                                                                 >  2:2689819/1‑93 (MQ=255)

                         gATGCCCAGACTGCACGGCCTGCCAGGAATCCCGATGCGCCCGCCGTCATTGCCACGCGTACGGCGCGCGGGAACAGTTTTTCGTCGACACCg                                                                >  2:5781846/1‑93 (MQ=255)

                         caTGCCCAGACTGCACGGCCTGCCAGGAATCCCGATGCGCCCGCCGTCATTGCCACGCGTACGGCGCGCGGGAACAGTTTTTCGTCGACACCg                                                                <  1:4171792/92‑1 (MQ=255)

                           tGCCCAGACTGCACGGCCTGCCAGGAATCCCGATGCGCCCGCCGTCATTGCCACGCGTACGGCGCGCGGGAACAGTTTTTCGTCGacac                                                                  >  2:4814912/1‑89 (MQ=255)

                           tGCCCAGACTGCACGGCCTGCCAGGAATCCCGATGCGCCCGCCGTCATTGCCACGCGTACGGCGCGCGGGAACAGTTTTTCGTCGACACCGGa                                                              <  1:1089891/93‑1 (MQ=255)

                           tGCCCAGACGGCACGCCCTGCCAGAAACCCCGATGCGCCCGCCGTCATTGCCACGCGTACGGCGCGCGGGAACAGTTTTTCGTCGACACCGGa                                                              <  2:4006701/93‑1 (MQ=255)

                            gCCCAGACTGCACGGCCTGCCAGGAATCCCGATGCGCCCGCCGTCATTGCCACGCGTACGGCGCGCGGGAACAGTTTTTCGTCGACACCGGaa                                                             >  2:1922135/1‑93 (MQ=255)

                              ccAGACTGCACGGCCTGCCAGGAATCCCGATGCGCCCGCCGTCATTGCCACGCGTACGGCGCGCGGGAACAGTTTTTCGTCGACACCGGAaga                                                           >  1:2747500/1‑93 (MQ=255)

                              ccAGACTGCACGGCCTGCCAGGAATCCCGATGCGCCCGCCGTCATTGCCACGCGTACGGCGCGCGGGAACAGTTTTTCGTCGACACCGGAaga                                                           >  2:5987918/1‑93 (MQ=255)

                               cAGCCTCCACGCCCTGCCAGGAATCCCGATGCGCCCGCCGTCATTGCCACGCGTACGGCGCGCGGGAACAGTTTTTCGTCGACACCGGAagag                                                          <  2:132459/93‑1 (MQ=255)

                               cAGACTGCACGGCCTGCCAGGAATCCCGATGCGCCCGCCGTCATTGCCACGCGTACGGCGCGCGGGAACAGTTTTTCGTCGACACCGGAagag                                                          <  2:5015633/93‑1 (MQ=255)

                                aGACTGCACGGCCTGCCAGGAATCCCGATGCGCCCGCCGTCATTGCCACGCGTACGGCGCGCGGGAACAGTTTTTCGTCGACACCGGAagaga                                                         >  2:1002019/1‑93 (MQ=255)

                                aGACTGCACGGCATGCCGGGAATCCCGATGCGCCCGCCGTCATTGCCACGCGTACGGCGCGCGGGAACAGTTTTTCGTCGACACCGGAagaga                                                         <  2:3656541/93‑1 (MQ=255)

                                 gACTGCACGGCCTGCCAGGAATCCCGATGCGCCCGCCGTCATTGCCACGCGTACGGCGCGCGGGAACAGTTTTTCGTCGACACCGGAagagag                                                        >  2:226239/1‑93 (MQ=255)

                                 gACTGCACGGCCTGCCAGGAATCCCGATGCGCCCGCCGTCATTGCCACGCGTACGGCGCGCGGGAACAGTTTTTCGTCGACACCGGAagagag                                                        >  2:2066902/1‑93 (MQ=255)

                                   cTGCACGGCCTGCCAGGAATCCCGATGCGCCCGCCGTCATTGCCACGCGTACGGCGCGCGGGAACAGTTTTTCGTCGACACCGGAAGAGAGGa                                                      <  2:795738/93‑1 (MQ=255)

                                    tGCACGGCCTGCCAGGAATCCCGATGCGCCCGCCGTCATTGCCACGCGTACGGCGCGCGGGAACAGTTTTTCGTCGACACCGGAAGAGAGGAt                                                     >  2:5482694/1‑93 (MQ=255)

                                    tGCACGGCCTGCCAGGAATCCCGATGCGCCCGCCGTCATTGCCACGCGTACGGCGCGCGGGAACAGTTTTTCGTCGACACCGGAAGAGAGGAt                                                     <  1:2652843/93‑1 (MQ=255)

                                      cACGGCCTGCCAGGAATCCCGATGCGCCCGCCGTCATTGCCACGCGTACGGCGCGCGGGAACAGTTTTTCGTCGACACCGGAAGAGAGGATCa                                                   >  1:5121594/1‑93 (MQ=255)

                                      cACGGCCTGCCAGGAATCCCGATGCGCCCGCCGTCATTGCCACGCGTACGGCGCGCGGGAACAGTTTTTCGTCGACACCGGAAGAGAGGATCa                                                   >  2:3774493/1‑93 (MQ=255)

                                          gCCTGCCAGGAATCCCGATGCGCCCGCCGTCATTGCCACGCGTACGGCGCGCGGGAACAGTTTTTCGTCGACACCGGAAGAGAGGATCACCCa                                               <  2:4897260/93‑1 (MQ=255)

                                          gCCTGCCAGGAATCCCGATGCGCCCGCCGTCATTGCCACGCGTACGGCGCGCGGGAACAGTTTTTCGTCGACACCGGAAGAGAGGATCACCCa                                               <  1:1161420/93‑1 (MQ=255)

                                          gCCTGCCAGGAATCCCGATGCGCCCGCCGTCATTGCCACGCGTACGGCGCGCGGGAACAGTTTTTCGTCGACACCGGAAGAGAGGATCACCCa                                               >  2:334439/1‑93 (MQ=255)

                                           ccggCCAGAAATCCCGATGCGCCCGCCGTCATTGCCACGCGTACGGCGCGCGGGAACAGTTTTTCGTCGACACCGGAAGAGAGGATCACCCAt                                              <  1:2455161/90‑1 (MQ=255)

                                            cTGCCAGGAATCCCGATGCGCCCGCCGTCATTGCCACGCGTACGGCGCGCGGGAACAGTTTTTCGTCGACACCGGAAGAGAGGATCACCCATg                                             >  1:6155716/1‑93 (MQ=255)

                                            cTGCCAGGAATCCCGATGCGCCCGCCGTCATTGCCACGCGTACGGCGCGCGGGAACAGTTTTTCGTCGACACCGGAAGAGAGGATCACCCATg                                             >  1:2939561/1‑93 (MQ=255)

                                             ggCCAGGAACCCCGATGCGCCCGCCGTCATTGCCACGCGTACGGCGCGCGGGAACAGTTTTTCGTCGACACCGGAAGAGAGGATCACCCATgg                                            <  1:3443569/92‑1 (MQ=255)

                                              gCCAGGAGTCCCGATGCGCCCGCCGTCATTGCCACGCGTACGGCGCGCGGGAACAGTTTTTCGTCGACACCGGAAGAGAGGATCACCCATGGc                                           <  1:2534029/93‑1 (MQ=255)

                                               ccAGGAATCCCGATGCGCCCGCCGTCATTGCCACGCGTACGGCGCGCGGGAACAGTTTTTCGTCGACACCGGAAGAGAGGATCACCCATGGCa                                          <  2:416688/93‑1 (MQ=255)

                                                cAGGAATCCCGATGCGCCCGCCGTCATTGCCACGCGTACGGCGCGCGGGAACAGTTTTTCGTCGACACCGGAAGAGAGGATCACCCATGGCat                                         >  1:2475601/1‑93 (MQ=255)

                                                 aGGAATCCCGATGCGCCCGCCGTCATTGCCACGCGTACGGCGCGCGGGAACAGTTTTTCGTCGACACCGGAAGAGAGGATCACCCATGGCata                                        <  2:5271695/93‑1 (MQ=255)

                                                 aGGAAACCCGATGCCCCCGCCGTCATTGCCACGCGTACGGCGCGCGGGAACAGTTTTTCGTCGACACCGGAAGAGAGGATCACCCATGGCata                                        <  1:3750439/93‑1 (MQ=255)

                                                  ggAATCCCGATGCGCCCGCCGTCATTGCCACGCGTACGGCGCGCGGGAACAGTTTTTCGTCGACACCGGAAGAGAGGATCACCCATGGCatat                                       >  2:3709075/1‑93 (MQ=255)

                                                   gAATCCCGATGCGCCCGCCGTCATTGCCACGCGTACGGCGCGCGGGAACAGTTTTTCGTCGACACCGGAAGAGAGGATCACCCATGGCATAtt                                      >  1:1646950/1‑93 (MQ=255)

                                                   gAATCCCGATGCGCCCGCCGTCATTGCCACGCGTACGGCGCGCGGGAACAGTTTTTCGTCGACACCGGAAGAGAGGATCACCCATGGCATAtt                                      <  2:572172/93‑1 (MQ=255)

                                                    aaTCCCGATGCGCCCGCCGTCATTGCCACGCGTACGGCGCGCGGGAACAGTTTTTCGTCGACACCGGAAGAGAGGATCACCCATGGCATATTg                                     <  1:1254925/93‑1 (MQ=255)

                                                     aTCCCGATGCGCCCGCCGTCATTGCCACGCGTACGGCGCGCGGGAACAGTTTTTCGTCGACACCGGGAGAGAGGATCACCCATGGCATATTGa                                    >  1:2330997/1‑93 (MQ=255)

                                                     aTCCCGATGCGCCCGCCGTCATTGCCACGCGTACGGCGCGCGGGAACAGTTTTTCGTCGACACCGGAAGAGAGGATCACCCATGGCATATTGa                                    <  1:4571391/93‑1 (MQ=255)

                                                      gcccGATGCGCCCGCCGTCATTGCCACGCGTACGGCGCGCGGGAACAGTTTTTCGTCGACACCGGAAGAGAGGATCACCCATGGCATATTGat                                   <  1:3917244/92‑1 (MQ=255)

                                                       cccGATGCGCCCGCCGTCATTGCCACGCGTACGGCGCGCGGGAACAGTTTTTCGTCGACACCGGAAGAGAGGATCACCCATGGCATATTGata                                  <  1:87475/93‑1 (MQ=255)

                                                       cccGATGCGCCCGCCGTCATTGCCACGCGTACGGCGCGCGGGAACAGTTTTTCGTCGACACCGGAAGAGAGGATCACCCATGGCATATTGata                                  <  1:4626043/93‑1 (MQ=255)

                                                        ccGATGCGCCCGCCGTCATTGCCACGCGTACGGCGCGCGGGAGCAGTTTTTCGTCGACACCGGAAGAGAGGATCACCCATGGCATATTGatat                                 >  1:3453289/1‑93 (MQ=255)

                                                        ccGATGCGCCCGCCGTCATTGCCACGCGTACGGCGCGCGGGAACAGTTTTTCGTCGACACCGGAAGAGAGGATCACCCATGGCATATTGatat                                 <  1:4957934/93‑1 (MQ=255)

                                                           aTGCGCCCGCCGTCATTGCCACGCGTACGGCGCGCGGGAACAGTTTTTCGTCGACACCGGAAGAGAGGATCACCCATGGCATATTGATATGGt                              >  2:4231360/1‑93 (MQ=255)

                                                            tGCGCCCGCCGTCATTGCCACGCGTACGGCGCGCGGGAACAGTTTTTCGTCGACACCGGAAGAGAGGATCACCCATGGCATATTGATATGGTc                             <  1:476761/93‑1 (MQ=255)

                                                             gcgcCCGCCGTCATTGCCACGCGTACGGCGCGCGGGAACAGTTTTTCGTCGACACCGGAAGAGAGGATCACCCATGGCATATTGATATGGTCa                            <  2:1587281/93‑1 (MQ=255)

                                                              cgcccgccGTCATTGCCACGCGTACGGCGCGCGGGAACAGTTTTTCGTCGACACCGGAAGAGAGGATCACCCATGGCATATTGATATGGTCAt                           >  1:2133708/1‑93 (MQ=255)

                                                                cccgccGTCATTGCCACGCGTACGGCGCGCGGGAACAGTTTTTCGTCGACACCGGAAGAGAGGATCACCCATGGCATATTGATATGGTCATTc                         <  1:5831314/93‑1 (MQ=255)

                                                                cccgccGTCATTGCCACGCGTACGGCGCGCGGGAACAGTTTTTCGTCGACACCGGAAGAGAGGATCACCCATGGCATATTGATATGGTCATTc                         <  1:5195674/93‑1 (MQ=255)

                                                                cccgccGTCATTGCCACGCGTACGGCGCGCGGGAACAGTTTTTCGTCGACACCGGAAGAGAGGATCACCCATGGCATATTGATATGGTCATTc                         <  2:6296670/93‑1 (MQ=255)

                                                                 ccgccgTCATTGCCACGCGTACGGCGCGCGGGAACAGTTTTTCGTCGACACCGGAAGAGAGGATCACCCATGGCATATTGATATGGTCATTCa                        >  2:2606127/1‑93 (MQ=255)

                                                                 ccgccgTCATTGCCACGCGTACGGCGCGCGGGAACAGTTTTTCGTCGACACCGGAAGAGAGGATCACCCATGGCATATTGATATGGTCATTCa                        >  2:2131581/1‑93 (MQ=255)

                                                                  cgccgTCATTGCCACGCGTACGGCGCGCGGGAACAGTTTTTCGTCGACACCGGAAGAGAGGATCACCCATGGCATATTGATATGGTCATTCAg                       >  2:5542310/1‑93 (MQ=255)

                                                                  cgccgTCATTGCCACGCGTACGGCGCGCGGGAACAGTTTTTCGTCGACACCGGAAGAGAGGATCACCCATGGCATATTGATATGGTCATTCAg                       >  2:4936266/1‑93 (MQ=255)

                                                                   gccgTCATTGCCACGCGTACGGCGCGCGGGAACAGTTTTTCGTCGACACCGGAAGAGAGGATCACCCATGGCATATTGATATGGTCATTCAGa                      <  2:3481547/93‑1 (MQ=255)

                                                                    ccgTCATTGCCACGCGTACGGCGCGCGGGAACAGTTTTTCGTCGACACCGGAAGAGAGGATCACCCATGGCATATTGATATGGTCATTCAGAc                     >  2:4571434/1‑93 (MQ=255)

                                                                     cgTCATTGCCACGCGTACGGCGCGCGGGAACAGTTTTTCGTCGACACCGGAAGAGAGGATCACCCATGGCATATTGATATGGTCATTCAGACg                    <  1:309980/93‑1 (MQ=255)

                                                                     cgTCATTGCCACGCGTACGGCGCGCGGGAACAGTTTTTCGTCGACACCGGAAGAGAGGATCACCCATGGCATATTGATATGGTCATTCAGACg                    >  2:4597358/1‑93 (MQ=255)

                                                                      gTCATTGCCACGCGTACGGCGCGCGGGAACAGTTTTTCGTCGACACCGGAAGAGAGGATCACCCATGGCATATTGATATGGTCATTCAGACGt                   <  1:4482874/93‑1 (MQ=255)

                                                                      gTCATTGCCACGCGTACGGCGCGCGGAAACAGTTTTTCGTCGACACCGGAAGAGAGGATCACCCATGGCATATTGATATGGTCATTCAGACGt                   <  1:416533/93‑1 (MQ=255)

                                                                       tCATTGCCACGCGTACGGCGCGCGTGAACAGTTTTTCGTCGACACCGGAAGAGAGGATCACCCATGGCATATTGATATGGTCATTCAGACGtt                  >  1:1560883/1‑93 (MQ=255)

                                                                       tCATTGCCACGCGTACGGCGCGCGGGAACAGTTTTTCGTCGACACCGGAAGAGAGGATCACCCATGGCATATTGATATGGTCATTCAGACGtt                  <  1:5993200/93‑1 (MQ=255)

                                                                       tCATTGCCACGCGTACGGCGCGCGGGAACAGTTTTTCGTCGACACCGGAAGAGAGGATCACCCATGGCATATTGATATGGTCATTCAGACGtt                  <  1:6128804/93‑1 (MQ=255)

                                                                       tCATTGCCACGCGTACGGCGCGCGGGAACAGTTTTTCGTCGACACCGGAAGAGAGGATCACCCATGGCATATTGATATGGTCATTCAGACGtt                  >  1:5529517/1‑93 (MQ=255)

                                                                       tCATTGCCACGCGTACGGCGCGCGGGAACAGTTTTTCGTCGACACCGGAAGAGAGGATCACCCATGGCATATTGATATGGTCATTCAGACGtt                  >  1:5439510/1‑93 (MQ=255)

                                                                       tCATTGCCACGCGTACGGCGCGCGGGAACAGTTTTTCGTCGACACCGGAAGAGAGGATCACCCATGGCATATTGATATGGTCATTCAGACGtt                  >  1:506290/1‑93 (MQ=255)

                                                                         aTTGCCACGCGTACGGCGCGCGGGAACAGTTTTTCGTCGACACCGGAAGAGAGGATCACCCATGGCATATTGATATGGTCATTCAGACGTtgt                >  2:622002/1‑93 (MQ=255)

                                                                          ttGCCACGCGTACGGCGCGCGGGAACAGTTTTTCGTCGACACCGGAAGAGAGGATCACCCATGGCATATTGATATGGTCATTCAGACGTtgtg               >  2:3187148/1‑93 (MQ=255)

                                                                          ttGCCACGCGTACGGCGCGCGGGAACAGTTTTTCGTCGACACCGGAAGAGAGGATCACCCATGGCATATTGATATGGTCATTCAGACGTtgtg               <  1:4808791/93‑1 (MQ=255)

                                                                            gCCACGCGTACGGCGCGCGGGAACAGTTTTTCGTCGACACCGGAAGAGAGGATCACCCATGGCATATTGATATGGTCATTCAGACGTTGTGaa             <  1:2610901/93‑1 (MQ=255)

                                                                              cACGCGTACGGCGCGCGGGAACAGTTTTTCGTCGACACCGGAAGAGAGGATCACCCATGGCATATTGATATGGTCATTCAGACGTTGTGAAGc           <  1:1501185/93‑1 (MQ=255)

                                                                              cACGCGTACGGCGCGCGGGAACAGTTTTTCGTCGACACCGGAAGAGAGGATCACCCATGGCATATTGATATGGTCATTCAGACGTTGTGAAGc           <  2:2677287/93‑1 (MQ=255)

                                                                                  cgTACGGCGCGCGGGAACAGTTTTTCGTCGACACCGGAAGAGAGGATCACCCATGGCATATTGATATGGTCATTCAGACGTTGTGAAGCACag       <  1:6036158/93‑1 (MQ=255)

                                                                                     aCGGCGCGCGGGAACAGTTTTTCGTCGACACCGGAAGAGAGGATCACCCATGGCATATTGATATGGTCATTCAGACGTTGTGAAGCACAgaga    <  1:5682787/93‑1 (MQ=255)

                                                                                     aCGGCGCGCGGGAACAGTTTTTCGTCGACACCGGAAGAGAGGATCACCCATGGCATATTGATATGGTCATTCAGACGTTGTGAAGCACAgaga    <  1:2289550/93‑1 (MQ=255)

                                                                                     aCGGCGCGCGGGAACAGTTTTTCGTCGACACCGGAAGAGAGGATCACCCATGGCATATTGATATGGTCATTCAGACGTTGTGAAGCACAgaga    <  2:663869/93‑1 (MQ=255)

                                                                                     aCGGCGCGCGGGAACAGTTTTTCGTCGACACCGGAAGAGAGGATCACCCATGGCATATTGATATGGTCATTCAGACGTTGTGAAGCACAgaga    <  1:1431909/93‑1 (MQ=255)

                                                                                      cGGCGCGCGGGAACAGTTTTTCGTCGACACCGGAAGAGAGGATCACCCATGGCATATTGATATGGTCATTCAGACGTTGTGAAGCACAGAGaa   <  1:927548/93‑1 (MQ=255)

                                                                                       ggCGCGCGGGAACAGTTTTTCGTCGACACCGGAAGAGAGGATCACCCATGGCATATTGAGATGGTCATTCAGACGTTGTGACGCATAGAGAAg  >  2:2912289/1‑93 (MQ=255)

CTCGTTGTCTGGTAAACCGACGACCGATGCCCAGACTGCACGGCCTGCCAGGAATCCCGATGCGCCCGCCGTCATTGCCACGCGTACGGCACGCGGGAACAGTTTTTCGTCGACACCGGAAGAGAGGATCACCCATGGCATATTGATATGGTCATTCAGACGTTGTGAAGCACAGAGAAG  >  NC_000913/4069434‑4069613

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 20 ≤` `ATCG/ATCG` `< 29 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

BRESEQ :: bam2aln output

CAGCTCGTTGTCTGGTAAACCGACGACCGATGCCCAGACTGCACGGCCTGCCAGGAATCCCGATGCGCCCGCCGTCATTGCCACGCGTACGGCACGCGGGAACAGTTTTTCGTCGACACCGGAAGAGAGGATCACCCATGGCATATTGATATGGTCATTCAGACGTTGTGAAGCACAGAGAAGCTCT  >  NC_000913/4069431‑4069617

CAGCTCGTTGTCTGGTAAACCGACGACCGATGCCCAGACTGCACGGCCTGCCAGGAATCCCGATGCGCCCGCCGTCATTGCCACGCGTACGGCG                                                                                               <  SRR4302537.2314185/94‑1 (MQ=60)

CAGCTCGTTGTCTGGTAAACCGACGACCGATGCCCAGACTGCACGGCCTGCCAGGAATCCCGATGCGCCCGCCGTCATTGCCACGCGTACGGCG                                                                                               >  SRR4302537.1960206/1‑94 (MQ=60)

 AGCTCGTTGTCTGGTAAACCGACGACCGATGCCCAGACTGCACGGCCTGCCAGGAATCCCGATGCGCCCGCCGTCATTGCCACGCGTACGGCGC                                                                                              >  SRR4302537.1522472/1‑94 (MQ=60)

   CTCGTTGTCTGGTAAACCGACGACCGATGCCCAGACTGCACGGCCTGCCAGGAATCCCGATGCGCCCGCCGTCATTGCCACGCGTACGGCGCGC                                                                                            >  SRR4302537.3307380/1‑94 (MQ=60)

     CGTTGTCGGGTAAACCGACGACCGATGCCCAGACTGCACGGCCTGCCAGGAATCCCGATGCGCCCGCCGTCATTGCCACGCGTACGGCGCGCGG                                                                                          <  SRR4302537.2258830/94‑1 (MQ=60)

      GTTGTCTGGTAAACCGACGACCGATGCCCAGACTGCACGGCCTGCCAGGAATCCCGATGCGCCCGCCGTCATTGCCACGCGTACGGCGCGCGGG                                                                                         >  SRR4302537.1978966/1‑94 (MQ=60)

      GTTGTCTGGTAAACCGACGACCGATGCCCAGACTGCACGGCCTGCCAGGAATCCCGATGCGCCCGCCGTCATTGCCACGCGTACGGCGCGCGGG                                                                                         <  SRR4302537.4726459/94‑1 (MQ=60)

        TGTCTGGTAAACCGACGACCGATGCCCAGACTGCACGGCCTGCCAGGAATCCCGATGCGCCCGCCGTCATTGCCACGCGTACGGCGCGCGGGAA                                                                                       >  SRR4302537.796124/1‑94 (MQ=60)

         GTCTGGTAAACCGACGACCGATGCCCAGACTGCACGGCCTGCCAGGAATCCCGATGCGCCCGCCGTCATTGCCACGCGTACGGCGCGCGGGAAC                                                                                      >  SRR4302537.882673/1‑94 (MQ=60)

         GTCTGGTAAACCGACGACCGATGCCCAGACTGCACGGCCTGCCAGGAATCCCGATGCGCCCGCCGTCATTGCCACGCGTACGGCGCGCGGGAAC                                                                                      >  SRR4302537.3727445/1‑94 (MQ=60)

         GTCTGGTAAACCGACGACCGATGCCCAGACTGCACGGCCTGCCAGGAATCCCGATGCGCCCGCCGTCATTGCCACGCGTACGGCGCGCGGGAAC                                                                                      >  SRR4302537.1999070/1‑94 (MQ=60)

          TCTGGTAAACCGACGACCGATGCCCAGACTGCACGGCCTGCCAGGAATCCCGATGCGCCCGCCGTCATTGCCACGCGTACGGCGCGCGTGAACA                                                                                     >  SRR4302537.1987026/1‑94 (MQ=60)

           CTGGTAAACCGACGACCGATGCCCAGACTGCACGGCCTGCCAGGAATCCCGATGCGCCCGCCGTCATTGCCACGCGTACGGCGCGCGGGAACAG                                                                                    >  SRR4302537.2733468/1‑94 (MQ=60)

           CTGGTAAACCGACGACCGATGCCCAGACTGCACGGCCTGCCAGGAATCCCGATGCGCCCGCCGTCATTGCCACGCGTACGGCGCGCGGGAACAG                                                                                    >  SRR4302537.3044103/1‑94 (MQ=60)

           CTGGTAAACCGACGACCGATGCCCAGACTGCACGGCCTGCCAGGAATCCCGATGCGCCCGCCGTCATTGCCACGCGTACGGCGCGCGGGAACAG                                                                                    >  SRR4302537.2488379/1‑94 (MQ=60)

           CTGGTAAACCGACGACCGATGCCCAGACTGCACGGCCTGCCAGGAATCCCGATGCGCCCGCCGTCATTGCCACGCGTACCGCGCGCGGCAACAG                                                                                    >  SRR4302537.4456797/1‑94 (MQ=60)

               GAAACCGACGACCGATGCCCAGACTGCACGGCCTGCCAGGAATCCCGATGCGCCCGCCGTCATTGCCACGCGTACGGCGCGCGGGAACAGTTTT                                                                                <  SRR4302537.2046833/94‑1 (MQ=60)

                 AACCGACGACCGATGCCCAGACTGCCCGGCCTGCCAGGAATCCCGATGCGCCCGCCGTCATTGCCACGCGTACGGCGCGCGGGAACAGTTTTTC                                                                              <  SRR4302537.4538559/94‑1 (MQ=60)

                  ACCGCCGACGAATGCCCAGACTGCACGGCCTGCCGGGATTCCCGAGGCGCCCGCCGTCATTGCCACGCGTACGGCGCGCGGGAACAGTTTTTCG                                                                             <  SRR4302537.381281/94‑1 (MQ=60)

                  ACCGACGACCGATGCCCAGACTGCACGGCCTGCCAGGAATCCCGATGCGCCCGCCGTCATTGCCACGCGTACGGCGCGCGGGAACAGTTTTTCG                                                                             <  SRR4302537.2428854/94‑1 (MQ=60)

                   CCGACGACCGATGCCCAGACTGCACGGCCTGCCAGGAATCCCGATGCGCCCGCCGTCATTGCCACGCGTACGGCGCGCGGGAACAGTTTTTCGT                                                                            <  SRR4302537.5584002/94‑1 (MQ=60)

                    CGACGACCGATGCCCAGACTGCACGGCCTGCCAGGAATCCCGATGCGCCCGCCGTCATTGCCACGCGTACGGCGCGCGGGAACAGTTTTTCGTC                                                                           >  SRR4302537.4291986/1‑94 (MQ=60)

                    CGACGACCGATGCCCAGACTGCACGGCCTGCCAGGAATCCCGATGCGCCCGCCGTCATTGCCACGCGTACGGCGCGCGGGAACAGTTTTTCGTC                                                                           >  SRR4302537.4291987/1‑94 (MQ=60)

                    CGACGACCGATGCCCAGACTGCACGGCCTGCCAGGAATCCCGATGCGCCCGCCGTCATTGCCACGCGTACGGCGCGCGGGAACAGTTTTTCGTC                                                                           >  SRR4302537.3498337/1‑94 (MQ=60)

                     GACGACCGATGCCCAGACTGCACGGCCTGCCAGGAATCCCGATGCGCCCGCCGTCATTGCCACGCGTACGGCGCGCGGGAACAGTTTTTCGTCG                                                                          <  SRR4302537.2526416/94‑1 (MQ=60)

                       CGACCGATGCCCAGACTGCACGGCCTGCCACGAATCCCGATGCGCCCGCCGTCATTGCCACGCGTACGGCGCGCGGGAACAGTTTTTCGTCGAC                                                                        <  SRR4302537.4516676/94‑1 (MQ=60)

                        GACCGATGCCCAGCCTGCACGGCCTGCCAGGAATCCCGATGCGCCCGCCGTCATTGCCACGCGTACGGCGCGCGGGAACAGTTTTTCGTCGACA                                                                       <  SRR4302537.3015247/94‑1 (MQ=60)

                         ACCGATGCCCAGACTGCACGGCCTGCCAGGAATCCCGATGCGCCCGCCGTCATTGCCACGCGTACGGCGCGCGGGAACAGTTTTTCGTCGACAC                                                                      <  SRR4302537.3117639/94‑1 (MQ=60)

                          cgtATGCCCAGACTGCACGGCCTGCCAGGAATCCCGATGCGCCCGCCGTCATTGCCACGCGTACGGCGCGCGGGAACAGTTTTTCGTCGACACC                                                                     <  SRR4302537.4848145/91‑1 (MQ=60)

                          CCGATGCCCAGACTGCACGGCCTGCCAGGAATCCCGATGCGCCCGCCGTCATTGCCACGCGTACGGCGCGCGGGAACAGTTTTTCGTCGACACC                                                                     <  SRR4302537.1817819/94‑1 (MQ=60)

                           CGATGCCCAGACTGCACGGCCTGCCAGGAATCCCGATGCGCCCGCCGTCATTGCCACGCGTACGGCGCGCGGGAACAGTTTTTCGTCGACACCG                                                                    <  SRR4302537.5892369/94‑1 (MQ=60)

                           CGATGCCCAGACGGCACGGCCTGCCAGGAATCCCGATGCGCCCGCCGTCATTGCCACGCGTACGGCGCGCGGGAACAGTTTTTCGTCGACACCG                                                                    <  SRR4302537.5664307/94‑1 (MQ=60)

                            CATGCCCAGACTGCACGGCCTGCCAGGAATCCCGATGCGCCCGCCGTCATTGCCACGCGTACGGCGCGCGGGAACAGTTTTTCGTCGACACCGG                                                                   <  SRR4302537.4200927/94‑1 (MQ=60)

                              TGCCCAGACTGCACGGCCTGCCAGGAATCCCGATGCGCCCGCCGTCATTGCCACGCGTACGGCGCGCGGGAACAGTTTTTCGTCGACACCGGAA                                                                 <  SRR4302537.1097417/94‑1 (MQ=60)

                               gccgagctggcacgtcaTGCCAGGAACCCCGATGCTCCCGCCGTCATTGCCACGCGTACGGCGCGCGGGAACAGTTTTTCGTCGACACCGGAAG                                                                <  SRR4302537.1132996/77‑1 (MQ=60)

                               GCCCAGACTGCACGGCCTGCCAGGAATCCCGATGCGCCCGCCGTCATTGCCACGCGTACGGCGCGCGGGAACAGTTTTTCGTCGACACCGGAAG                                                                >  SRR4302537.3976886/1‑94 (MQ=60)

                                CCCAGACTGCACGGCCTGCCAGGAATCCCGATGCGCCCGCCGTCATTGCCACGCGTACGGCGCGCGGGAACAGTTTTTCGTCGACACCGGAAGA                                                               <  SRR4302537.3271642/94‑1 (MQ=60)

                                CCCAGACTGCACGGCCTGCCAGGAATCCCGATGCGCCCGCCGTCATTGCCACGCGTACGGCGCGCGGGAACAGTTTTTCGTCGACACCGGAAGA                                                               <  SRR4302537.3377897/94‑1 (MQ=60)

                                CCCAGACTGCACGGCCTGCCAGGAATCCCGATGCGCCCGCCGTCATTGCCACGCGTACGGCGCGCGGGAACAGTTTTTCGTCGACACCGGAAGA                                                               >  SRR4302537.2766664/1‑94 (MQ=60)

                                  CAGCCTGCGCGGCCTGCCAGGAATCCCGATGCGCCCGCCGTCATTGCCACGCGTACGGCGCGCGGGAACAGTTTTTCGTCGACACCGGAAGAGA                                                             <  SRR4302537.4833250/94‑1 (MQ=60)

                                    GACTGCACGGCCTGCCAGGAACCCCGATGCGCCCGCCGTCATTGCCACGCGTACGGCGCGCGGGAACAGTTTTTCGTCGACACCGGAAGAGAGG                                                           <  SRR4302537.1683321/94‑1 (MQ=60)

                                     ACTGCACGGCCGGCCAGGAATCCCGATGCGCCCGCCGTCATTGCCACGCGTACGGCGCGCGGGAACAGTTTTTCGTCGACACCGGAAGAGAGGA                                                          <  SRR4302537.5036874/94‑1 (MQ=60)

                                      CTGCACGGCCTGCCAGGAATCCCGATGCGCCCGCCGTCATTGCCACGCGTACGGCGCGCGGGAACAGTTTTTCGTCGACACCGGAAGAGAGGAT                                                         >  SRR4302537.2924254/1‑94 (MQ=60)

                                       TGCACGGCCTGCCAGGAATCCCGATGCGCCCGCCGTCATTGCCACGCGTACGGCGCGCGGGAACAGTTTTTCGTCGACACCGGAAGAGAGGATC                                                        <  SRR4302537.2626351/94‑1 (MQ=60)

                                       TGCACGGCCTGCCAGGAATCCCGATGCGCCCGCCGTCATTGCCACGCGTACGGCGCGCGGGAACAGTTTTTCGTCGACACCGGAAGAGAGGATC                                                        <  SRR4302537.2671390/94‑1 (MQ=60)

                                        cccgcGCCTGCCAGGAAGCCCCAGGCGCCCGCCGTCATTGCCACGCGTACGGCGCGCGGAAACAGTTTTTCGTCGACACCGGAAGAGAGGATCA                                                       <  SRR4302537.1097686/89‑1 (MQ=60)

                                        GCACGGCCTGCCAGGAATCCCGATGCGCCCGCCGTCATTGCCACGCGTACGGCGCGCGGGAACAGTTTTTCGTCGACACCGGAAGAGAGGATCA                                                       >  SRR4302537.5156894/1‑94 (MQ=60)

                                         CACGGCCTGCCAGGAATCCCGATGCGCCCGCCGTCATTGCCACGCGTACGGCGCGCGGGAACAGTTTTTCGTCGACACCGGAAGAGAGGATCAC                                                      >  SRR4302537.6065805/1‑94 (MQ=60)

                                            gtctcgccgtgaacCCCGATGCTCCCGCCGTCATTGCCGCGCGTACGGCGCGCGGAAACAGTTTTTCGTCGACACCGGAAGAGAGGATCACCCA                                                   <  SRR4302537.6056162/80‑1 (MQ=60)

                                             GCCTGCGAGGAGGCCCGCTGCCCCCGCCATCATTGCCACGCGTACGGCCCGCGGGATCAGTTTTTCGTCGACACCGGAAGAGAGGATCACCCAT                                                  <  SRR4302537.6181739/94‑1 (MQ=60)

                                             GCCTGCCAGGAATCCCGATGCGCCCGCCGTCATTGCCACGCGTACGGCGCGCGGGAACAGTTTTTCGTCGACACCGGAAGAGAGGATCACCCAT                                                  >  SRR4302537.2011834/1‑94 (MQ=60)

                                             GCCTGCCAGGAATCCCGATGCGCCCGCCGTCATTGCCACGCGTACGGCGCGCGGGAACAGTTTTTCGTCGACACCGGAAGAGAGGATCACCCAT                                                  <  SRR4302537.1169497/94‑1 (MQ=60)

                                              CCTGCCAGGAATCCGGATGCGCCCGCCGTCATTGCCACGCGTACGGCGCGCGGGAACAGTTTTTCGTCGACACCGGAAGAGAGGATCACCCATG                                                 <  SRR4302537.3980978/94‑1 (MQ=60)

                                              CCTGCCAGGAATCCCGATGCGCCCGCCGTCATTGCCACGCGTACGGCGCGCGGGAACAGTTTTTCGTCGACACCGGAAGAGAGGATCACCCATG                                                 >  SRR4302537.6198069/1‑94 (MQ=60)

                                              CCTGCCAGGAATCCCGATGCGCCCGCCGTCATTGCCACGCGTACGGCGCGCGGGAACAGTTTTTCGTCGACACCGGAAGAGAGGATCACCCATG                                                 >  SRR4302537.2960199/1‑94 (MQ=60)

                                              CCTGCCAGGAATCCCGATGCGCCCGCCGTCATTGCCACGCGTACGGCGCGCGGGAACAGTTTTTCGTCGACACCGGAAGAGAGGATCACCCATG                                                 >  SRR4302537.1507308/1‑94 (MQ=60)

                                              CCTGCAAGGAATCCCGATGCGCCCGCCGTCATTGCCACGCGTACGGCGCGCGGGAACAGTTTTTCGTCGACACCGGAAGAGAGGATCACCCATG                                                 <  SRR4302537.6132392/94‑1 (MQ=60)

                                              CCGGCCAGAAATCCCGATGCGCCCGCCGTCATTGCCACGCGTACGGCGCGCGGGAACAGTTTTTCGTCGACACCGGAAGAGAGGATCACCCATG                                                 <  SRR4302537.2472429/94‑1 (MQ=60)

                                                GGCCAGGAACCCCGATGCGCCCGCCGTCATTGCCACGCGTACGGCGCGCGGGAACAGTTTTTCGTCGACACCGGAAGAGAGGATCACCCATGGC                                               <  SRR4302537.3467932/94‑1 (MQ=60)

                                                 GCCAGGAGTCCCGATGCGCCCGCCGTCATTGCCACGCGTACGGCGCGCGGGAACAGTTTTTCGTCGACACCGGAAGAGAGGATCACCCATGGCA                                              <  SRR4302537.2551813/94‑1 (MQ=60)

                                                  CCAGGAATCCCGATGCGCCCGCCGTCATTGCCACGCGTACGGCGCGCGGGAACAGTTTTTCGTCGACACCGGAAGAGAGGATCACCCATGGCAT                                             >  SRR4302537.794822/1‑94 (MQ=60)

                                                  CCAGGAATCCCGATGCGCCCGCCGTCATTGCCACGCGTACGGCGCGCGGGAACAGTTTTTCGTCGACACCGGAAGAGAGGATCACCCATGGCAT                                             <  SRR4302537.2921561/94‑1 (MQ=60)

                                                  CCAGGAATCCCGATGCGCCCGCCGTCATTGCCACGCGTACGGCGCGCGGGAACAGTTTTTCGTCGACACCGGAAGAGAGGATCACCCATGGCAT                                             >  SRR4302537.2493003/1‑94 (MQ=60)

                                                    AGGAATCCCGATGCGCCCGCCGTCATTGCCACGCGTACGGCGCGCGGGAACAGTTTTTCGTCGACACCGGAAGAGAGGATCACCCATGGCATAT                                           >  SRR4302537.5109072/1‑94 (MQ=60)

                                                    AGGAAACCCGATGCCCCCGCCGTCATTGCCACGCGTACGGCGCGCGGGAACAGTTTTTCGTCGACACCGGAAGAGAGGATCACCCATGGCATAT                                           <  SRR4302537.3776777/94‑1 (MQ=60)

                                                     GGAATCCCGATGCGCCCGCCGTCATTGCCACGCGTACGGCGCGCGGGAACAGTTTTTCGTCGACACCGGAAGAGAGGATCACCCATGGCATATT                                          <  SRR4302537.5183030/94‑1 (MQ=60)

                                                     GGAATCCCGATGCGCCCGCCGTCATTGCCACGCGTACGGCGCGCGGGAACAGTTTTTCGTCGACACCGGAAGAGAGGATCACCCATGGCATATT                                          >  SRR4302537.1658486/1‑94 (MQ=60)

                                                      GAATCCCGATGCGCCCGCCGTCATTGCCACGCGTACGGCGCGCGGGAACAGTTTTTCGTCGACACCGGAAGAGAGGATCACCCATGGCATATTG                                         >  SRR4302537.239786/1‑94 (MQ=60)

                                                      GAATCCCGATGCGCCCGCCGTCATTGCCACGCGTACGGCGCGCGGGAACAGTTTTTCGTCGACACCGGAAGAGAGGATCACCCATGGCATATTG                                         <  SRR4302537.3812534/94‑1 (MQ=60)

                                                       AATCCCGATGCGCCCGCCGTCATTGCCACGCGTACGGCGCGCGGGAACAGTTTTTCGTCGACACCGGGAGAGAGGATCACCCATGGCATATTGA                                        >  SRR4302537.2347430/1‑94 (MQ=60)

                                                       AATCCCGATGCGCCCGCCGTCATTGCCACGCGTACGGCGCGCGGGAACAGTTTTTCGTCGACACCGGAAGAGAGGATCACCCATGGCATATTGA                                        <  SRR4302537.1263658/94‑1 (MQ=60)

                                                       AATCCCGATGCGCCCGCCGTCATTGCCACGCGTACGGCGCGCGGGAACAGTTTTTCGTCGACACCGGAAGAGAGGATCACCCACGGCATATTGA                                        >  SRR4302537.419805/1‑94 (MQ=60)

                                                        ATCCCGATGCGCCCGCCGTCATTGCCACGCGTACGGCGCGCGGGAACAGTTTTTCGTCGACACCGGAAGAGAGGATCACCCATGGCATATTGAT                                       <  SRR4302537.2198112/94‑1 (MQ=60)

                                                        ATCCCGATGCGCCCGCCGTCATTGCCACGCGTACGGCGCGCGGGAACAGTTTTTCGTCGACACCGGAAGAGAGGATCACCCATGGCATATTGAT                                       <  SRR4302537.1563612/94‑1 (MQ=60)

                                                        ATCCCGATGCGCCCGCCGTCATTGCCACGCGTACGGCGCGCGGGAACAGTTTTTCGTCGACACCGGAAGAGAGGATCACCCATGGCATATTGAT                                       <  SRR4302537.4603036/94‑1 (MQ=60)

                                                         TCCCGATGCGCCCGCCGTCATTGCCACGCGTACGGCGCGCGGGAACAGTTTTTCGTCGACACCGGAAGAGAGGATCACCCATGGCATATTGATA                                      <  SRR4302537.5108812/94‑1 (MQ=60)

                                                         TCCCGATGCGCCCGCCGTCATTGCCACGCGTACGGCGCGCGGGAACAGTTTTTCGTCGACACCGGAAGAGAGGATCACCCATGGCATATTGATA                                      <  SRR4302537.5561799/94‑1 (MQ=60)

                                                         GCCCGATGCGCCCGCCGTCATTGCCACGCGTACGGCGCGCGGGAACAGTTTTTCGTCGACACCGGAAGAGAGGATCACCCATGGCATATTGATA                                      <  SRR4302537.3944622/94‑1 (MQ=60)

                                                          CCCGATGCGCCCGCCGTCATTGCCACGCGTACGGCGCGCGGGAGCAGTTTTTCGTCGACACCGGAAGAGAGGATCACCCATGGCATATTGATAT                                     >  SRR4302537.3477710/1‑94 (MQ=60)

                                                          CCCGATGCGCCCGCCGTCATTGCCACGCGTACGGCGCGCGGGAACAGTTTTTCGTCGACACCGGAAGAGAGGATCACCCATGGCATATTGATAT                                     <  SRR4302537.4658054/94‑1 (MQ=60)

                                                          CCCGATGCGCCCGCCGTCATTGCCACGCGTACGGCGCGCGGGAACAGTTTTTCGTCGACACCGGAAGAGAGGATCACCCATGGCATATTGATAT                                     <  SRR4302537.3786768/94‑1 (MQ=60)

                                                          CCCGATGCGCCCGCCGTCATTGCCACGCGTACGGCGCGCGGGAACAGTTTTTCGTCGACACCGGAAGAGAGGATCACCCATGGCATATTGATAT                                     <  SRR4302537.88108/94‑1 (MQ=60)

                                                           CCGATGCGCCCGCCGTCATTGCCACGCGTACGGCGCGCGGGAACAGTTTTTCGTCGACACCGGAAGAGAGGATCACCCATGGCATATTGATATG                                    <  SRR4302537.4992114/94‑1 (MQ=60)

                                                            CGATGCGCCCGCCGTCATTGCCACGCGTACGGCGCGCGGGAACAGTTTTTCGTCGACACCGGAAGAGAGGATCACCCATGGCATATTGATATGG                                   >  SRR4302537.2067171/1‑94 (MQ=60)

                                                            CGATGCGCCCGCCGTCATTGCCACGCGTACGGCGCGCGGGAACAGTTTTTCGTCGACACCGGAAGAGAGGATCACCCATGGCATATTGATATGG                                   <  SRR4302537.3350934/94‑1 (MQ=60)

                                                               TGCGCCCGCCGTCATTGCCACGCGTACGGCGCGCGGGAACAGTTTTTCGTCGACACCGGAAGAGAGGATCACCCATGGCATATTGATATGGTCA                                <  SRR4302537.3754967/94‑1 (MQ=60)

                                                               TGCGCCCGCCGTCATTGCCACGCGTACGGCGCGCGGGAACAGTTTTTCGTCGACACCGGAAGAGAGGATCACCCATGGCATATTGATATGGTCA                                <  SRR4302537.480017/94‑1 (MQ=60)

                                                               TGCGCCCGCCGTCATTGCCACGCGTACGGCGCGCGGGAACAGTTTTTCGTCGACACCGGAAGAGAGGATCACCCATGGCATATTGATATGGTCA                                <  SRR4302537.3067046/94‑1 (MQ=60)

                                                                GCGCCCGCCGTCATTGCCACGCGTACGGCGCGCGGGAACAGTTTTTCGTCGACACCGGAAGAGAGGATCACCCATGGCATATTGATATGGTCAT                               >  SRR4302537.2148727/1‑94 (MQ=60)

                                                                  GCCCGCCGTCATTGCCACGCGTACGGCGCGCGGGAACAGTTTTTCGTCGACACCGGAAGAGAGGATCACCCATGGCATATTGATATGGTCATTC                             >  SRR4302537.1427666/1‑94 (MQ=60)

                                                                  GCCCGCCGTCATTGCCACGCGTACGGCGCGCGGGAACAGTTTTTCGTCGACACCGGAAGAGAGGATCACCCATGGCATATTGATATGGTCATTC                             >  SRR4302537.1549329/1‑94 (MQ=60)

                                                                   CCCGCCGTCATTGCCACGCGTACGGCGCGCGGGAACAGTTTTTCGTCGACACCGGAAGAGAGGATCACCCATGGCATATTGATATGGTCATTCA                            <  SRR4302537.5231392/94‑1 (MQ=60)

                                                                   CCCGCCGTCATTGCCACGCGTACGGCGCGCGGGAACAGTTTTTCGTCGACACCGGAAGAGAGGATCACCCATGGCATATTGATATGGTCATTCA                            <  SRR4302537.5871279/94‑1 (MQ=60)

                                                                   CCCGCCGTCATTGCCACGCGTACGGCGCGCGGGAACAGTTTTTCGTCGACACCGGAAGAGAGGATCACCCATGGCATATTGATATGGTCATTCA                            <  SRR4302537.2501443/94‑1 (MQ=60)

                                                                      GCCGTCATTGCCACGCGTACGGCGCGCGGGAACAGTTTTTCGTCGACACCGGAAGAGAGGATCACCCATGGCATATTGATATGGTCATTCAGAC                         <  SRR4302537.6179408/94‑1 (MQ=60)

                                                                      CCCGTCACTGCCACGCGTATGGCGCGCGGGAACACTTTTTCGTCGACACCGGAAGAGAGGATCACCCATGGCATATTGATATGGTCATTCAGAC                         <  SRR4302537.5421129/94‑1 (MQ=60)

                                                                       CCGTCATTGCCACGCGTACGGCGCGCGGGAACAGTTTTTCGTCGACACCGGAAGAGAGGATCACCCATGGCATATTGATATGGTCATTCAGACG                        <  SRR4302537.1264478/94‑1 (MQ=60)

                                                                       CCGTCATTGCCACGCGTACGGCGCGCGGGAACAGTTTTTCGTCGACACCGGAAGAGAGGATCACCCATGGCATATTGATATGGTCATTCAGACG                        <  SRR4302537.3908496/94‑1 (MQ=60)

                                                                        CGTCATTGCCACGCGTACGGCGCGCGGGAACAGTTTTTCGTCGACACCGGAAGAGAGGATCACCCATGGCATATTGATATGGTCATTCAGACGT                       <  SRR4302537.2661574/94‑1 (MQ=60)

                                                                        CGTCATTGCCACGCGTACGGCGCGCGGGAACAGTTTTTCGTCGACACCGGAAGAGAGGATCACCCATGGCATATTGATATGGTCATTCAGACGT                       <  SRR4302537.312126/94‑1 (MQ=60)

                                                                        CGTCATTGCCACGCGTACGGCGCGCGGGAACAGTTTTTCGTCGACACCGGAAGAGAGGATCACCCATGGCATATTGATATGGTCATTCAGACGT                       >  SRR4302537.3470992/1‑94 (MQ=60)

                                                                         GTCATTGCCACGCGTACGGCGCGCGTGAACAGTTTTTCGTCGACACCGGAAGAGAGGATCACCCATGGCATATTGATATGGTCATTCAGACGTT                      >  SRR4302537.1571776/1‑94 (MQ=60)

                                                                         GTCATTGCCACGCGTACGGCGCGCGGGAACAGTTTTTCGTCGACACCGGAAGAGAGGATCACCCATGGCATATTGATATGGTCATTCAGACGTT                      >  SRR4302537.110835/1‑94 (MQ=60)

                                                                         GTCATTGCCACGCGTACGGCGCGCGGGAACAGTTTTTCGTCGACACCGGAAGAGAGGATCACCCATGGCATATTGATATGGTCATTCAGACGTT                      >  SRR4302537.509764/1‑94 (MQ=60)

                                                                         GTCATTGCCACGCGTACGGCGCGCGGGAACAGTTTTTCGTCGACACCGGAAGAGAGGATCACCCATGGCATATTGATATGGTCATTCAGACGTT                      >  SRR4302537.5476617/1‑94 (MQ=60)

                                                                         GTCATTGCCACGCGTACGGCGCGCGGGAACAGTTTTTCGTCGACACCGGAAGAGAGGATCACCCATGGCATATTGATATGGTCATTCAGACGTT                      >  SRR4302537.6111361/1‑94 (MQ=60)

                                                                         GTCATTGCCACGCGTACGGCGCGCGGGAACAGTTTTTCGTCGACACCGGAAGAGAGGATCACCCATGGCATATTGATATGGTCATTCAGACGTT                      >  SRR4302537.5567264/1‑94 (MQ=60)

                                                                         GTCATTGCCACGCGTACGGCGCGCGGGAACAGTTTTTCGTCGACACCGGAAGAGAGGATCACCCATGGCATATTGATATGGTCATTCAGACGTT                      <  SRR4302537.4513885/94‑1 (MQ=60)

                                                                         GTCATTGCCACGCGTACGGCGCGCGGAAACAGTTTTTCGTCGACACCGGAAGAGAGGATCACCCATGGCATATTGATATGGTCATTCAGACGTT                      <  SRR4302537.419425/94‑1 (MQ=60)

                                                                          TCATTGCCACGCGTACGGCGCGCGGGAACAGTTTTTCGTCGACACCGGAAGAGAGGATCACCCATGGCATATTGATATGGTCATTCAGACGTTG                     <  SRR4302537.6170927/94‑1 (MQ=60)

                                                                          TCATTGCCACGCGTACGGCGCGCGGGAACAGTTTTTCGTCGACACCGGAAGAGAGGATCACCCATGGCATATTGATATGGTCATTCAGACGTTG                     <  SRR4302537.6034363/94‑1 (MQ=60)

                                                                          TCATTGCCACGCGTACGGCGCGCGGGAACAGTTTTTCGTCGACACCGGAAGAGAGGATCACCCATGGCATATTGATATGGTCATTCAGACGTTG                     >  SRR4302537.3662432/1‑94 (MQ=60)

                                                                             TTGCCACGCGTACGGCGCGCGGGAACAGTTTTTCGTCGACACCGGAAGAGAGGATCACCCATGGCATATTGATATGGTCATTCAGACGTTGTGA                  <  SRR4302537.4841981/94‑1 (MQ=60)

                                                                              TGCCACGCGTACGGCGCGCGGGAACAGTTTTTCGTCGACACCGGAAGAGAGGATCACCCATGGCATATTGATATGGTCATTCAGACGTTGTGAA                 <  SRR4302537.1748746/94‑1 (MQ=60)

                                                                              TGCCACGCGTACGGCGCGCGGGAACAGTTTTTCGTCGACACCGGAAGAGAGGATCACCCATGGCATATTGATATGGTCATTCAGACGTTGTGAA                 >  SRR4302537.4648592/1‑94 (MQ=60)

                                                                               GCCACGCGTACGGCGCGCGGGAACAGTTTTTCGTCGACACCGGAAGAGAGGATCACCCATGGCATATTGATATGGTCATTCAGACGTTGTGAAG                <  SRR4302537.2629163/94‑1 (MQ=60)

                                                                                CCACGCGTACGGCGCGCGGGAACAGTTTTTCGTCGACACCGGAAGAGAGGATCACCCATGGCATATTGATATGGTCATTCAGACGTTGTGAAGC               <  SRR4302537.1648126/94‑1 (MQ=60)

                                                                                 CACGCGTACGGCGCGCGGGAACAGTTTTTCGTCGACACCGGAAGAGAGGATCACCCATGGCATATTGATATGGTCATTCAGACGTTGTGAAGCA              <  SRR4302537.2636573/94‑1 (MQ=60)

                                                                                 CACGCGTACGGCGCGCGGGAACAGTTTTTCGTCGACACCGGAAGAGAGGATCACCCATGGCATATTGATATGGTCATTCAGACGTTGTGAAGCA              <  SRR4302537.1511664/94‑1 (MQ=60)

                                                                                    GCGTACGGCGCGCGGGAACAGTTTTTCGTCGACACCGGAAGAGAGGATCACCCATGGCATATTGATATGGTCATTCAGACGTTGTGAAGCACAG           <  SRR4302537.5260799/94‑1 (MQ=60)

                                                                                     CGTACGGCGCGCGGGAACAGTTTTTCGTCGACACCGGAAGAGAGGATCACCCATGGCATATTGATATGGTCATTCAGACGTTGTGAAGCACAGA          <  SRR4302537.6077631/94‑1 (MQ=60)

                                                                                      GTACGGCGCGCGGGAACAGTTTTTCGTCGACACCGGAAGAGAGGATCACCCATGGCATATTGATATGGTCATTCAGACGTTGTGAAGCCCAGAG         >  SRR4302537.4154112/1‑94 (MQ=60)

                                                                                      GTACGGCGCGCGGGAACAGTTTTTCGTCGACACCGGAAGAGAGGATCACCCATGGCATATTGATATGGTCATTCAGACGTTGTGAAGCACAGAG         >  SRR4302537.5835321/1‑94 (MQ=60)

                                                                                      GTACGGCGCGCGGGAACAGTTTTTCGTCGACACCGGAAGAGAGGATCACCCATGGCATATTGATATGGTCATTCAGACGTTGTGAAGCACAGAG         <  SRR4302537.5989105/94‑1 (MQ=60)

                                                                                      GTACGGCGCGCGGGAACAGTTTTTCGTCGACACCGGAAGAGAGGATCACCCATGGCATATTGATATGGTCATTCAGACGTTGTGAAGCACAGAG         >  SRR4302537.423571/1‑94 (MQ=60)

                                                                                       TACGGCGCGCGGGAACAGTTTTTCGTCGACACCGGAAGAGAGGATCACCCATGGCATATTGATATGGTCATTCAGACGTTGTGAAGCACAGAGA        <  SRR4302537.2312260/94‑1 (MQ=60)

                                                                                        GCGGCGCGCGGGAACAGTTTTTCGTCGACACCGGAAGAGAGGATCACCCATGGCATATTGATATGGTCATTCAGACGTTGTGAAGCACAGAGAA       <  SRR4302537.4756555/94‑1 (MQ=60)

                                                                                        ACGGCGCGCGGGAACAGTTTTTCGTCGACACCGGAAGAGAGGATCACCCATGGCATATTGATATGGTCATTCAGACGTTGTGAAGCACAGAGAA       >  SRR4302537.955129/1‑94 (MQ=60)

                                                                                        ACGGCGCGCGGGAACAGTTTTTCGTCGACACCGGAAGAGAGGATCACCCATGGCATATTGATATGGTCATTCAGACGTTGTGAAGCACAGAGAA       <  SRR4302537.2305716/94‑1 (MQ=60)

                                                                                        ACGGCGCGCGGGAACAGTTTTTCGTCGACACCGGAAGAGAGGATCACCCATGGCATATTGATATGGTCATTCAGACGTTGTGAAGCACAGAGAA       <  SRR4302537.1441875/94‑1 (MQ=60)

                                                                                        ACGGCGCGCGGGAACAGTTTTTCGTCGACACCGGAAGAGAGGATCACCCATGGCATATTGATATGGTCATTCAGACGTTGTGAAGCACAGAGAA       <  SRR4302537.123259/94‑1 (MQ=60)

                                                                                        ACGGCGCGCGGGAACAGTTTTTCGTCGACACCGGAAGAGAGGATCACCCATGGCATATTGATATGGTCATTCAGACGTTGTGAAGCACAGAGAA       <  SRR4302537.5721657/94‑1 (MQ=60)

                                                                                         CGGCGCGCGGGAACAGTTTTTCGTCGACACCGGAAGAGAGGATCACCCATGGCATATTGATATGGTCATTCAGACGTTGTGAAGCACAGAGAAG      <  SRR4302537.4970048/94‑1 (MQ=60)

                                                                                         CGGCGCGCGGGAACAGTTTTTCGTCGACACCGGAAGAGAGGATCACCCATGGCATATTGATATGGTCATTCAGACGTTGTGAAGCACAGAGAAG      <  SRR4302537.933940/94‑1 (MQ=60)

                                                                                           GCGCGCGGGAACAGTTTTTCGTCGACACCGGAAGAGAGGATCACCCATGGCATATTGATATGGTCATTCAGACGTTGTGAAGCACAGAGAAGCT    <  SRR4302537.4182031/94‑1 (MQ=60)

                                                                                           GCGCGCGGGAACAGTTTTTCGTCGACACCGGAAGAGAGGATCACCCATGGCATATTGATATGGTCATTCAGACGTTGTGAAGCACAGAGAAGCT    <  SRR4302537.4862930/94‑1 (MQ=60)

                                                                                           GCGCGCGGGAACAGTTTTTCGTCGACACCGGAAGAGAGGATCACCCATGGCATATTGATATGGTCATTCAGACGTTGTGAAGCACAGAGAAGCT    <  SRR4302537.2886269/94‑1 (MQ=60)

                                                                                            CGCGCGGGAACAGTTTTTCGTCGACACCGGAAGAGAGGATCACCCATGGCATATTGATATGGTCATTCAGACGTTGTGAAGCACAGAGAAGCTC   <  SRR4302537.2753684/94‑1 (MQ=60)

                                                                                             GCGCGGGAACAGTTTTTCGTCGACACCGGAAGAGAGGATCACCCATGGCATATTGATATGGTCATTCAGACGTTGTGAAGCACAGAGAAGCTCT  <  SRR4302537.800920/94‑1 (MQ=60)

CAGCTCGTTGTCTGGTAAACCGACGACCGATGCCCAGACTGCACGGCCTGCCAGGAATCCCGATGCGCCCGCCGTCATTGCCACGCGTACGGCACGCGGGAACAGTTTTTCGTCGACACCGGAAGAGAGGATCACCCATGGCATATTGATATGGTCATTCAGACGTTGTGAAGCACAGAGAAGCTCT  >  NC_000913/4069431‑4069617

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 0 ≤` `ATCG/ATCG` `< 2 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`