Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I2 R1 882 254.4 12957588 98.0% 12698436 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 4,127,152 A→G S301G (AGT→GGT)  metB → cystathionine gamma‑synthase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,127,1520AG95.6% 714.0 / 15.7 227S301G (AGT→GGT) metBcystathionine gamma‑synthase
Reads supporting (aligned to +/- strand):  ref base A (6/4);  new base G (117/100);  total (123/104)
Fisher's exact test for biased strand distribution p-value = 7.57e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.13e-02

Only 100 of 231 total aligned reads displayed.

TTGGTGAAAAAACTGTATCACCCGTCGTTGCCGGAAAATCAGGGGCATGAAATTGCCGCGCGCCAGCAAAAAGGCTTTGGCGCAATGTTGAGTTTTGAACTGGATGGCGATGAGCAGACGCTGCGTCGTTTCCTGGGCGGGCTGTCGTTGTTTACGCTGGCGGAATCATTAGGGGGAGTG  >  NC_000913/4127062‑4127241
                                                                                          |                                                                                         
ttGGTGAAAAAACTGTATCACCCGTCGTTGCCGGAAAATCAGGGGCATGAAATTGCCGCGCGCCAGCAAAAAGGCTTTGGCGCAATGTTGGGt                                                                                         >  1:3770857/1‑93 (MQ=255)
  ggTGAAAAAGCTGTATCCCCCGTCGTTGCCGGAAAATCAGGGGCATGAAATTGCCGCGCGCCAGCAAAAAGGCTTTGGCGCAATGTTGGGttt                                                                                       <  2:2669873/93‑1 (MQ=255)
   gTGAAAAAACTGTATCACCCGTCGTTGCCGGAAAATCAGGGGCATGAAATTGCCGCGCGCCAGCAAAAAGGCTTTGGCGCAATGTTGGGtttt                                                                                      >  2:3272654/1‑93 (MQ=255)
     gAAAAAACTGTATCACCCGTCGTTGCCGGAAAATCAGGGGCATGAAATTGCCGCGCGCCAGCAAAAAGGCTTTGGCGCAATGTTGGGTTTTGa                                                                                    <  2:3972673/93‑1 (MQ=255)
      aaaaaaCTGTATCACCCGTCGTTGCCGGAAAATCAGGGGCATGAAATTGCCGCGCGCCAGCAAAAAGGCTTTGGCGCAATGTTGGGTTTTGaa                                                                                   <  2:1881242/93‑1 (MQ=255)
      aaaaaaCTGTATCACCCGTCGTTGCCGGAAAATCAGGGGCATGAAATTGCCGCGCGCCAGCAAAAAGGCTTTGGCGCAATGTTGGGTTTTGaa                                                                                   <  1:5967734/93‑1 (MQ=255)
           aCTGTATCACCCGTCGTTGCCGGAAAATCAGGGGCATGAAATTGCCGCGCGCCAGCAAAAAGGCTTTGGCGCAATGTTGGGTTTTGAACTGGa                                                                              >  1:1933290/1‑93 (MQ=255)
           aCTGTATCACCCGTCGTTGCCGGAAAATCAGGGGCATGAAATTGCCGCGCGCCAGCAAAAAGGCTTTGGCGCAATGTTGGGTTTTGAACTGGa                                                                              <  2:4121258/93‑1 (MQ=255)
           aCTGTATCACCCGTCGTTGCCGGAAAATCAGGGGCATGAAATTGCCGCGCGCCAGCAAAAAGGCTTTGGCGCAATGTTGGGTTTTGAACTGGa                                                                              <  2:1607549/93‑1 (MQ=255)
            cTGTATCACCCGTCGTTGCCGGAAAATCAGGGGCATGAAATTGCCGCGCGCCAGCAAAAAGGCTTTGGCGCAATGTTGGGTTTTGAACTGGAt                                                                             <  2:5286187/93‑1 (MQ=255)
            cTGTATCACCCGTCGTTGCCGGAAAATCAGGGGCATGAAATTGCCGCGCGCCAGCAAAAAGGCTTTGGCGCAATGTTGGGTTTTGAACTGGAt                                                                             >  2:2489606/1‑93 (MQ=255)
             tGTATCACCCGTCGTTGCCGGAAAATCAGGGGCATGAAATTGCCGCGCGCCAGCAAAAAGGCTTTGGCGCAATGTTGGGTTTTGCACTGGATg                                                                            >  2:5113356/1‑93 (MQ=255)
             tGTATCACCCGTCGTTGCCGGAAAATCAGGGGCATGAAATTGCCGCGCGCCAGCAAAAAGGCTTTGGCGCAATGTTGGGTTTTGAACTGGATg                                                                            >  2:696815/1‑93 (MQ=255)
             tGTATCACCCGTCGTTGCCGGAAAATCAGGGGCATGAAATTGCCGCGCGCCAGCAAAAAGGCTTTGGCGCAATGTTGGGTTTTGAACTGGATg                                                                            <  1:2516865/93‑1 (MQ=255)
               tATCACCCGTCGTTGCCGGAAAATCAGGGGCATGAAATTGCCGCGCGCCAGCAAAAAGGCTTTGGCGCAATGTTGGGTTTTGAACTGGATGGc                                                                          >  2:3696881/1‑93 (MQ=255)
               tATCACCCGTCGTTGCCGGAAAATCAGGGGCATGAAATTGCCGCGCGCCAGCAAAAAGGCTTTGGCGCAATGTTGGGTTTTGAACTGGATGGc                                                                          <  2:925856/93‑1 (MQ=255)
               tATCACCCGTCGTTGCCGGAAAATCAGGGGCATGAAATTGCCGCGCGCCAGCAAAAAGGCTTTGGCGCAATGTTGGGTTTTGAACTGGATGGc                                                                          >  1:1131904/1‑93 (MQ=255)
               tATCACCCGTCGTTGCCGGAAAATCAGGGGCATGAAATTGCCGCGCGCCAGCAAAAAGGCTTTGGCGCAATGTTGGGTTTTGAACTGGATGGc                                                                          <  1:1021684/93‑1 (MQ=255)
               tATCACCCGTCGTTGCCGGAAAATCAGGGGCATGAAATTGCCGCGCGCCAGCAAAAAGGCTTTGGCGCAATGTTGGGATTTGAACTGGATGGc                                                                          >  2:951023/1‑93 (MQ=255)
                  cACCCGTCGTTGCCGGAAAATCAGGGGCATGAAATTGCCGCGCGCCAGCAAAAAGGCTTTGGCGGAATGGTGGGTTTTGAACTGGATGGCGAt                                                                       >  2:5062982/1‑93 (MQ=255)
                  cACCCGTCGTTGCCGGAAAATCAGGGGCATGAAATTGCCGCGCGCCAGCAAAAAGGCTTTGGCGCAATGTTGGGTTTTGAACTGGATGGCGAt                                                                       >  1:3307363/1‑93 (MQ=255)
                  cACCCGTCGTTGCCGGAAAATCAGGGGCATGAAATTGCCGCGCGCCAGCAAAAAGGCTTTGGCGCAATGTTGGGTTTTGAACTGGATGGCGAt                                                                       <  2:6006643/93‑1 (MQ=255)
                    cccGTCGTTGCCGGAAAATCAGGGGCATGAAATTGCCGCGCGCCAGCAAAAAGGCTTTGGCGCAATGTTGGGTTTTGAACTGGATGGCGATGa                                                                     >  2:5439148/1‑93 (MQ=255)
                     ccGTCGTTGCCGGAAAATCAGGGGCATGAAATTGCCGCGCGCCAGCAAAAAGGCTTTGGCGCAATGTTGGGTTTTGAACTGGATGGCGATGAg                                                                    <  1:5450647/93‑1 (MQ=255)
                       gtcgtTGCCGGAAAATCAGGGGCATGAAATTGCCGCGCGCCAGCAAAAAGGCTTTGGCGCAATGTTGGGTTTTGAACTGGATGGCGATGAGCa                                                                  >  1:5922654/1‑93 (MQ=255)
                        tcgtTGCCGGAAAATCAGGGGCATGAAATTGCCGCGCGCCAGCAAAAAGGCTTTGGCGCAATGTTGGGTTTTGAACTGGATGGCGATGAGCAg                                                                 <  2:3082345/93‑1 (MQ=255)
                        tcgtTGCCGGAAAATCAGGGGCATGAAATTGCCGCGCGCCAGCAAAAAGGCTTTGGCGCAATGTTGGGTTTTGAACTGGATGGCGATGAGCAg                                                                 >  2:2168759/1‑93 (MQ=255)
                        tcgtTGCCGGAAAATCAGGGGCATGAAATTGCCGCGCGCCAGCAAAAAGGCTTTGGCGCAATGTTGGGTTTTGAACTGGATGGCGATGAGCAg                                                                 >  2:1739065/1‑93 (MQ=255)
                        tcgtTGCCGGAAAATCAGGGGCATGAAATTGCCGCGCGCCAGCAAAAAGGCTTTGGCGCAATGTTGGGTTTTGAACTGGATGGCGATGAGCAg                                                                 >  1:796097/1‑93 (MQ=255)
                        tcgtTGCCGGAAAATCAGGGGCATGAAATTGCCGCGCGCCAGCAAAAAGGCTTTGGCGCAATGTTGGGTTTTGAACTGGATGGCGATGAGCAg                                                                 >  1:5215566/1‑93 (MQ=255)
                          gtTGCCGGAAAATCAGGGGCATGAAATTGCCGCGCGCCAGCAAAAAGGCTTTGGCGCAATGTTGGGTTTTGAACTGGATGGCGATGAGCAGAc                                                               >  2:2020347/1‑93 (MQ=255)
                           ttGCCGGAAAATCAGGGGCATGAAATTGCCGCGCGCCAGCAAAAAGGCTTTGGCGCAATGTTGGGTTTTGAACTGGATGGCGATGAGCAGACg                                                              >  1:424547/1‑93 (MQ=255)
                            tGCCGGAAAATCAGGGGCATGAAATTGCCGCGCGCCAGCAAAAAGGCTTTGGCGCAATGTTGGGTTTTGAACTGGATGGCGATGAGCAGACGc                                                             >  1:3260519/1‑93 (MQ=255)
                             gCCGGAAAATCAGGGGCATGAAATTGCCGCGCGCCAGCAAAAAGGCTTTGGCGCAATGTTGGGTTTTGAACTGGATGGCGATGAGCAGACGCt                                                            >  1:5961442/1‑93 (MQ=255)
                              ccGGAAAATCAGGGGCATGAAATTGCCGCGCGCCAGCAAAAAGGCTTTGGCGCAATGTTGGGTTTTGAACTGGATGGCGATGAGCAGACGCTg                                                           >  2:4654292/1‑93 (MQ=255)
                                 gAAAATCAGGGGCATGAAATTGCCGCGCGCCAGCAAAAAGGCTTTGGCGCAATGTTGGGTTTTGAACTGGATGGCGATGAGCAGACGCTGcgt                                                        <  1:5170794/93‑1 (MQ=255)
                                 gAAAATCAGGGGCATGAAATTGCCGCGCGCCAGCAAAAAGGCTTTGGCGCAATGTTGGGTTTTGAACTGGATGGCGATGAGCAGACGCTGcgt                                                        >  1:1294761/1‑93 (MQ=255)
                                  aaaaTCAGGGGCATGAAATTGCCGCGCGCCAGCAAAAAGGCTTTGGCGCAATGTTGAGTTTTGAACTGGATGGCGATGAGCAGACGCTGcgtc                                                       <  1:2763270/93‑1 (MQ=255)
                                   aaaTCAGGGGCATGAAATTGCCGCGCGCCAGCAAAAAGGCTTTGGCGCAATGTTGGGTTTTGAACTGGATGGCGATGAGCAGACGCTGcgtcg                                                      >  1:6392150/1‑93 (MQ=255)
                                     aTCAGGGGCATGAAATTGCCGCGCGCCAGCAAAAAGGCTTTGGCGCAATGTTGGGTTTTGAACTGGATGGCGATGAGCAGGCGCTGCGTCGtt                                                    <  1:2120593/93‑1 (MQ=255)
                                     aTCAGGGGCATGAAATTGCCGCGCGCCAGCAAAAAGGCTTTGGCGCAATGCTGGGTTTTGAACTGGATGGCGATGAGCAGACGCTGCGTCGtt                                                    <  1:4551053/93‑1 (MQ=255)
                                     aTCAGGGGCATGAAATTGCCGCGCGCAAGCAAAAAGGCTTTGGCGCAATGTTGGGTTTTGAACTGGATGGCGATGAGCAGACGCTGCGTCGtt                                                    <  1:1807499/93‑1 (MQ=255)
                                      tCAGGGGCATGAAATTGCCGCGCGCCAGCAAAAAGGCTTTGGCGCAATGTTGGGTTTTGAACTGGATGGCGATGAGCAGACGCTGCGTCGttt                                                   <  1:1655099/93‑1 (MQ=255)
                                           ggCATGAAATTGCCGCGCGCCAGCAAAAAGGCTTTGGCGCAATGTTGGGTTTTGAACTGGCTGGCGATGAGCAGACGCTGCGTCGTTTCCTgg                                              >  1:2865055/1‑93 (MQ=255)
                                           ggCATGAAATTGCCGCGCGCCAGCAAAAAGGCTTTGGCGCAATGTTGGGTTTTGAACTGGATGGCGATGAGCAGACGCTGCGTCGTTTCCTgg                                              >  2:6229123/1‑93 (MQ=255)
                                             cATGAAATTGCCGCGCGCCAGCAAAAAGGCTTTGGCGCAATGTTGGGTTTTGAACTGGATGGCGATGAGCAGACGCTGCGTCGTTTCCTgggc                                            >  2:3372744/1‑93 (MQ=255)
                                               tGAAATTGCCGCGCGCCAGCAAAAAGGCTTTGGCGCAATGTTGGGTTTTGAACTGGATGGCGATGAGCAGACGCTGCGTCGTTTCCTgggcgg                                          <  1:6254647/93‑1 (MQ=255)
                                                 aaaTTGCCGCGCGCCAGCAAAAAGGCTTTGGCGCAATGTTGGGTTTTGAACTGGATGGCGATGAGCAGACGCTGCGTCGTTTCCTgggcgggc                                        <  2:3168581/93‑1 (MQ=255)
                                                  aaTTGCCGCGCGCCAGCAAAAAGGCTTTGGCGCAATGTTGGGTTTTGAACTGGATGGCGATGAGCAGACGCTGCGTCGTTTCCTGGGCGGGCt                                       <  2:4587625/93‑1 (MQ=255)
                                                      gCCGCGCGCCAGCAAAAAGGCTTTGGCGCAATGTTGGGTTTTGAACTGGATGGCGATGAGCAGACGCTGCGTCGTTTCCTGGGCGGGCTGTCg                                   >  1:5526381/1‑93 (MQ=255)
                                                       gcgcgcgCCAGCAAAAAGGCTTTGGCGCAATGTTGGGTTTTGAACTGGATGGCGATGGGCAGACGCTGCGTCGTTTCCTGGGCGGGCTGTCgt                                  <  1:4622132/92‑1 (MQ=255)
                                                        cgcgcgCCAGCAAAAAGGCTTTGGCGCAATGTTGGGTTTTGAACTGGATGGCGATGAGCAGACGCTGCGTCGTTTCCTGGGCGGGCTGTCgtt                                 >  1:86509/1‑93 (MQ=255)
                                                         gcgcgcCAGCAAAAAGGCTTTGGCGCAATGTTGGGTTTTGAACTGGATGGCGATGAGCAGACGCTGCGTCGTTTCCTGGGCGGGCTGTCgttg                                <  2:855671/93‑1 (MQ=255)
                                                          cgcgcCAGCAAAAAGGCTTTGGCGCAATGTTGGGTTTTGAACTGGATGGCGATGAGCAGACGCTGCGTCGTTTCCTGGGCGGGCTGTCgttgt                               <  1:887328/93‑1 (MQ=255)
                                                          cgcgcCAGCAAAAAGGCTTTGGCGCAATGTTGGGTTTTGAACTGGATGGCGATGAGCAGACGCTGCGTCGTTTCCTGGGCGGGCTGTCgttgt                               >  2:6385740/1‑93 (MQ=255)
                                                           gcgcCAGCAAAAAGGCTTTGGCGCAATGTTGGGTTTTGAACTGGATGGCGATGAGCAGACGCTGCGTCGTTTCCTGGGCGGGATGTCgttgtt                              <  2:760096/93‑1 (MQ=255)
                                                              ccAGCAAAAAGGCTTTGGCGCAATGTTGGGTTTTGAACTGGATGGCGATGAGCAGACGCTGCGTCGTTTCCTGGGCGGGCTGTCGTTGTTTAc                           <  1:5482663/93‑1 (MQ=255)
                                                               cAGCAAAAAGGCTTTGGCGCAATGTTGGGTTTTGAACTGGATGGCGATGAGCAGACGCTGCGTCGTTTCCTGGGCGGGCTGTCGTTGTTTACg                          >  2:1146804/1‑93 (MQ=255)
                                                               cAGCAAAAAGGCTTTGGCGCAATGTTGGGTTTTGAACTGGATGGCGATGAGCAGACGCTGCGTCGTTTCCTGGGCGGGCTGTCGTTGTTTACg                          <  1:1755892/93‑1 (MQ=255)
                                                                aGCAAAAAGGCTTTGGCGCAATGTTGGGTTTTGAACTGGATGGCGATGAGCAGACGCTGCGTCGTTTCCTGGGCGGGCTGTCGTTGTTTACGc                         >  2:3622351/1‑93 (MQ=255)
                                                                aGCAAAAAGGCTTTGGCGCAATGTTGGGTTTTGAACTGGATGGCGATGAGCAGACGCCGCGTCGTTTCCTGGGCGGGCTGTCGTTGTTTACGc                         <  2:1002911/93‑1 (MQ=255)
                                                                 gCAAAAAGGCTTTGGCGCAATGTTGGGTTTTGAACTGGATGGCGATGAGCAGACGCTGCGTCGTTTCCTGGGCGGGCTGTCGTTGTTTACGCt                        <  2:5793810/93‑1 (MQ=255)
                                                                 gCAAAAAGGCTTTGGCGCAATGTTGGGTTTTGAACTGGATGGCGATGAGCAGACGCTGCGTCGTTTCCTGGGCGGGCTGTCGTTGTTTACGCt                        <  1:3610621/93‑1 (MQ=255)
                                                                   aaaaaGGCTTTGGCGCAATGTTGGGTTTTGAACTGGATGGCGATGAGCAGACGCTGCGTCGTTTCCTGGGCGGGCTGTCGTTGTTTACGCTgg                      <  1:6290276/93‑1 (MQ=255)
                                                                   aaaaaGGCTTTGGCGCAATGTTGAGTTTTGAACTGGATGGCGATGAGCAGACGCTGCGTCGTTTCCTGGGCGGGCTGTCGTTGTTTAAGCTgg                      >  1:1000599/1‑93 (MQ=255)
                                                                    aaaaGGCTTTGGCGCAATGTTGGGTTTTGAACTGGATGGCGATGAGCAGACGCTGCGTCGTTTCCTGGGCGGGCTGTCGTTGTTTACGCTGGc                     <  1:6098440/93‑1 (MQ=255)
                                                                    aaaaGGCTTTGGCGCAATGTTGGGTTTTGAACTGGATGGCGATGAGCAGACGCTGCGTCGTTTCCTGGGCGGGCTGTCGTTGTTTACGCTGGc                     >  2:4765136/1‑93 (MQ=255)
                                                                    aaaaGGCTTTGGCGCAATGTTGGGTTTTGAACTGGATGGCGATGAGCAGACGCTGCGTCGTTTCCTGGGCGGGCTGTCGTTGTTTACGCTGGc                     >  1:2823478/1‑93 (MQ=255)
                                                                     aaaGGCTTTGGCGCAATGTTGGGTTTTGAACTGGATGGCGATGAGCAGACGCTGCGTCGTTTCCTGGGCGGGCTGTCGTTGTTTACGCTGGCg                    >  2:2975966/1‑93 (MQ=255)
                                                                     aaaGGCTTTGGCGCAATGTTGGGTTTTGAACTGGATGGCGATGAGCAGACGCTGCGTCGTTTCCTGGGCGGGCTGTCGTTGTTTACGCTGGCg                    >  1:3622726/1‑93 (MQ=255)
                                                                      aaGGCTTTGGCGCAATGTTGGGTTTTGAACTGGATGGCGATGAGCAGACGCTGCGTCGTTTCCTGGGCGGGCTGTCGTTGTTTACGCTGGCgg                   >  2:2883821/1‑93 (MQ=255)
                                                                       aGGCTTTGGCGCAATGTTGGGTTTTGAACTGGATGGCGATGAGCAGACGCTGCGTCGTTTCCTGGGCGGGCTGTCGTTGTTTACGCTGGCGGa                  >  2:5846273/1‑93 (MQ=255)
                                                                        ggCTTCGGCGCAATGTTGGGTTATGAACTGGATGGCGATGAGCAGACGCTGCGTCGTTTCCTGGGCGGGCTGTCGTTGTTTACGCTGGCGGaa                 <  2:2922367/93‑1 (MQ=255)
                                                                         gCTTTGGCGCAATGTTGGGTTTTGAACTGGATGGCGATGAGCAGACGCTGCGTCGTTTCCTGGGCGGGCTGTCGTTGTTTACGCTGGCGGAAt                >  1:5009422/1‑93 (MQ=255)
                                                                         gCTTTGGCGCAATGTTGGGTTTTGAACTGGATGGCGATGAGCAGACGCTGCGTCGTTTCCTGGGCGGGCTGTCGTTGTTTACGCTGGCGGAAt                >  1:3103903/1‑93 (MQ=255)
                                                                         gCTTTGGCGCAATGTTGGGTTTTGAACTGGATGGCGATGAGCAGACGCTGCGTCGTTTCCTGGGCGGGCTGTCGTTGTTTACGCTGGCGGAAt                <  1:2036672/93‑1 (MQ=255)
                                                                         gCTTTGGCGCAATGTTGGGTTTTGAACTGGATGGCGATGAGCAGACGCTGCGTCGTTTCCTGGGCGGGCTGTCGTTGTTTACGCTGGCGGAAt                >  2:1343586/1‑93 (MQ=255)
                                                                          cTTTGGCGCAATGTTGGGTTTTGAACTGGATGGCGATGAGCAGACGCTGCGTCGTTTCCTGGGCGGGCTGTCGTTGTTTACGCTGGCGGAATc               <  2:1538115/93‑1 (MQ=255)
                                                                          cTTTGGCGCAATGTTGGGTTTTGAACTGGAGGGCGATGAGCAGACGCTGCGTCGTTTCCTGGGCGGGCTGTCGTTGTTTACGCTGGCGGAATc               <  1:6154894/93‑1 (MQ=255)
                                                                           tttGGCGGAATGTTGGGTTTTGAACTGGATGGCGATGAGCAGACGCTGCGTCGTTTCCTTGGCGGGCTGTTCGTGATTACGCTGGCGGTAtaa              >  2:5232395/1‑91 (MQ=255)
                                                                           tttGGCGCAATGTTGGGTTTTGAACTGGATGGCGATGAGCAGACGCTGCGTCGTTTCCTGGGCGGGCTGTCGTTGTTTACGCTGGCGGAATCa              >  1:267339/1‑93 (MQ=255)
                                                                           tttGGCGCAATGTTGGGTTTTGAACTGGATGGCGATGAGCAGACGCTGCGTCGTTTCCTGGGCGGGCTGTCGTTGTTTACGCTGGCGGAATCa              >  2:4209810/1‑93 (MQ=255)
                                                                           tttGGCGCAATGTTGGGTTTTGAACTGGATGGCGATGAGCAGACGCTGCGTCGTTTCCTGGGCGGGCTGTCGTTGTTTACGCTGGCGGAATCa              >  2:3141702/1‑93 (MQ=255)
                                                                           tttGGCGCAATGTTGGGTTTTGAACTGGATGGCGATGAGCAGACGCTGCGTCGTTTCCTGGGCGGGCTGTCGTTGTTTACGCTGGCGGAATCa              <  2:556906/93‑1 (MQ=255)
                                                                             tGGCGCAATGTTGGGTTTTGAACTGGATGGCGATGAGCAGACGCTGCGTCGTTTCCTGGGCGGGCTGTCGTTGTTTACGCTGGCGGAATCAtt            <  1:4029725/93‑1 (MQ=255)
                                                                             tGGCGCAATGTTGGGTTTTGAACTGGATGGCGATGAGCAGACGCTGCGTCGTTTCCTGGGCGGGCTGTCGTTGTTTACGCTGGCGGAATCAtt            <  1:4064559/93‑1 (MQ=255)
                                                                               gcgcAATGTTGGGTTTTGAACTGGATGGCGATGAGCAGACGCTGCGTCGTTTCCTGGGCGGGCTGTCGTTGTTTACGCTGGCGGAATCATTAg          <  2:2084932/93‑1 (MQ=255)
                                                                               gcgcAATGTTGGGTTTTGAACTGGATGGCGATGAGCAGACGCTGCGTCGTTTCCTGGGCGGGCTGTCGTTGTTTACGCTGGCGGAATCATTAg          >  1:5113813/1‑93 (MQ=255)
                                                                               gcgcAATGTTGGGTTTTGAACTGGATGGCGATGAGCAGACGCTGCGTCGTTTCCTGGGCGGGCTGTCGTTGTTTACGCTGGCGGAATCATTAg          >  2:2562082/1‑93 (MQ=255)
                                                                                cgcAATGTTGGGTTTTGAACTGGATGGCGATGAGCAGACGCTGCGTCGTTTCCTGGGCGGGCTGTCGTTGTTTACGCTGGCGGAATCATTAgg         <  1:2278813/93‑1 (MQ=255)
                                                                                cgcAATGTTGGGTTTTGAACTGGATGGCGATGAGCAGACGCTGCGTCGTTTCCTGGGCGGGCTGTCGTTGTTTACGCTGGCGGAATCATTAgg         >  2:3435115/1‑93 (MQ=255)
                                                                                 gcAATGTTGGGTTTTGAACTGGATGGCGATGAGCAGACGCTGCGTCGTTTCCTGGGCGGGCTGTCGTTGTTTACGCTGGCGGAATCATTAggg        >  1:6039212/1‑93 (MQ=255)
                                                                                  cAATGTTGGGTTTTGAACTGGATGGCGATGAGCAGACGCTGCGTCGTTTCCTGGGCGGGCTGTCGTTGTTTACGCTGGCGGAATCATTAgggg       <  1:4891232/93‑1 (MQ=255)
                                                                                   aaTGTTGGGTTTTGAACTGGATGGCGATGAGCAGACGCTGCGTCGTTTCCTGGGCGGGCTGTCGTTGTTTACGCTGGCGGAATCATTAggggg      >  2:3744248/1‑93 (MQ=255)
                                                                                   aaTGTTGAGTTTTGAACTGGATGGCGATGAGCAGACGCTGCGTCGTTTCCTGGGCGGGCTGTCGTTGTTTACGCTGGCGGAATCATTAggggg      >  2:5679629/1‑93 (MQ=255)
                                                                                    aTGTTGGGTTTTGAACTGGATGGCGATGAGCAGACGCTGCGTCGTTTCCTGGGCGGGCTGTCGTTGTTTACGCTGGCGGAATCATTAgggggg     >  2:4972913/1‑92 (MQ=255)
                                                                                    aTGTTGGGTTTTGAACTGGATGGCGATGAGCAGACGCTGCGTCGTTTCCTGGGCGGGCTGTCGTTGTTTACGCTGGCGGAATCATTAgggggg     >  2:4825495/1‑92 (MQ=255)
                                                                                     tGTTGGGTTTTGAACTGGATGGCGATGTGCAGACGCTGCGTCGTTTCCTGGGCGGGCTGTCGTTGTTTACGCTGGCGGAATCATTAGGGGGAg    >  1:2343267/1‑93 (MQ=255)
                                                                                      gTTGGGTTTTGAACTGGATGGCGATGAGCAGACGCTGCGTCGTTTCCTGGGCGGGCTGTCGTTGTTTACGCTGGCGGAATCATTAGGGGGAgg   >  2:3766744/1‑92 (MQ=255)
                                                                                       ttGGGTTTTGAACTGGATGGCGATGAGCAGACGCTGCGTCGTTTCCTGGGCGGGCTGTCGTTGTTTACGCTGGCGGAATCATTAGGGGGAGTg  >  1:670152/1‑93 (MQ=255)
                                                                                          |                                                                                         
TTGGTGAAAAAACTGTATCACCCGTCGTTGCCGGAAAATCAGGGGCATGAAATTGCCGCGCGCCAGCAAAAAGGCTTTGGCGCAATGTTGAGTTTTGAACTGGATGGCGATGAGCAGACGCTGCGTCGTTTCCTGGGCGGGCTGTCGTTGTTTACGCTGGCGGAATCATTAGGGGGAGTG  >  NC_000913/4127062‑4127241

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 18 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
CCGTTGGTGAAAAAACTGTATCACCCGTCGTTGCCGGAAAATCAGGGGCATGAAATTGCCGCGCGCCAGCAAAAAGGCTTTGGCGCAATGTTGAGTTTTGAACTGGATGGCGATGAGCAGACGCTGCGTCGTTTCCTGGGCGGGCTGTCGTTGTTTACGCTGGCGGAATCATTAGGGGGAGTGGAAA  >  NC_000913/4127059‑4127245
                                                                                             |                                                                                             
CCGTTGGTGAAAAAACTGTATCACCCGTCGTTGCCGGAAAATCAGGGGCATGAAATTGCCGCGCGCCAGCAAAAAGGCTTTGGCGCAATGTTGG                                                                                               <  SRR4302537.3166256/94‑1 (MQ=60)
CCGTTGGTGAAAAAACTGTATCACCCGTCGTTGCCGGAAAATCAGGGGCATGAAATTGCCGCGCGCCAGCAAAAAGGCTTTGGCGCAATGTTGG                                                                                               <  SRR4302537.591799/94‑1 (MQ=60)
CCGTTGGTGAAAAAACTGTATCACCCGTCGTTGCCGGAAAATCAGGGGCATGAAATTGCCGCGCGCCAGCAAAAAGGCTTTGGCGCAATGTTGG                                                                                               <  SRR4302537.1395244/94‑1 (MQ=60)
 CGTTGGTGAAAAAACTGTATCACCCGTCGTTGCCGGAAAATCAGGGGCATGAAATTGCCGCGCGCCAGCAAAAAGGCTTTGGCGCAATGTTGGG                                                                                              >  SRR4302537.3426323/1‑94 (MQ=60)
  GTTGGTGAAAAAACTGTATCACCCGTCGTTGCCGGAAAATCAGGGGCATGAAATTGCCGCGCGCCAGCAAAAAGGCTTTGGCGCAATGTTGGGT                                                                                             >  SRR4302537.3797328/1‑94 (MQ=60)
    TGGTGAAAAAACTGTATCACCCGTCGTTGTCGGAAAATCAGGGGCATGAAATTGCCGCGCGCCAGCAAAAAGGCTTTGGCGCAATGTTGGGTTT                                                                                           <  SRR4302537.3672666/94‑1 (MQ=60)
    TGGTGAAAAAACTGTATCACCCGTCGTTGCCGGAAAATCAGGGGCATGAAATTGCGGCGCGCCAGCAAAAAGGCTTTGGCGCAATGTTGGGTTT                                                                                           <  SRR4302537.5187567/94‑1 (MQ=60)
    TGGTGAAAAAACTGTATCACCCGTCGTTGCCGGAAAATCAGGGGCATGAAATTGCCGCGCGCCAGCAAAAAGGCTTTGGCGCAATGTTGGGTTT                                                                                           >  SRR4302537.3573303/1‑94 (MQ=60)
       TGAAAAAACTGTATCACCCGTCGTTGCCGGAAAATCAGGGGCATGAAATTGCCGCGCGCCAGCAAAAAGGCTTTGGCGCAATGTTGGGTTTTGA                                                                                        >  SRR4302537.5518977/1‑94 (MQ=60)
       TGAAAAAACTGTATCACCCGTCGTTGCCGGAAAATCAGGGGCATGAAATTGCCGCGCGCCAGCAAAAAGGCTTTGGCGCAATGTTGGGTTTTGA                                                                                        <  SRR4302537.891438/94‑1 (MQ=60)
        GAAAAAACTGTATCACCCGTCGTTGCCGGAAAATCAGGGGCATGAAATTGCCGCGCGCCAGCAAAAAGGCTTTGGCGCAATGTTGGGTTTTGAA                                                                                       >  SRR4302537.6387991/1‑94 (MQ=60)
         AAAAAACTGTATCACCCGTCGTTGCCGGAAAATCAGGGGCATGAAATTGCCGCGCGCCAGCAAAAAGGCTTTGGCGCAATGTTGGGTTTTGAAC                                                                                      <  SRR4302537.6008692/94‑1 (MQ=60)
         AAAAAACTGTATCACCCGTCGTTGCCGGAAAATCAGGGGCATGAAATTGCCGCGCGCCAGCAAAAAGGCTTTGGCGCAATGTTGGGTTTTGAAC                                                                                      >  SRR4302537.2117725/1‑94 (MQ=60)
          AAAAACTGTATCACCCGTCGTTGCCGGAAAATCAGGGGCATGAAATTGCCGCGCGCCAGCAAAAAGGCTTTGGCGCAATGTTGGGTTTTGAACT                                                                                     <  SRR4302537.2307020/94‑1 (MQ=60)
           AAAACTGTATCCCCCGTCGTTGCCGGAAAATCAGGGGCATGAAATTGCCGCGCGCCAGCAAAAAGGCTTTGGCGCAATGTTGGGTTTTGAACTG                                                                                    <  SRR4302537.5230138/94‑1 (MQ=60)
           AAAACTGTATCACCCGTCGTTGCCGGAAAATCAGGGGCATGAAATTGCCGCGCGCCAGCAAAAAGGCTTTGGCGCAATGTTGGGTTTTGAACTG                                                                                    >  SRR4302537.3828400/1‑94 (MQ=60)
             AACTGTATCACCCGTCGTTGCCGGAAAATCAGGGGCATGAAATTGCCGCGCGCCAGCAAAAAGGCTTTGGCGCAATGTTGGGTTTTGAACTGGA                                                                                  >  SRR4302537.1946734/1‑94 (MQ=60)
              ACTGTATCACCCGTCGTTGCCGGAAAATCAGGGGCATGAAATTGCCGCGCGCCAGCAAAAAGGCTTTGGCGCAATGTTGGGTTTTGAACTGGAT                                                                                 >  SRR4302537.4058585/1‑94 (MQ=60)
              ACTGTATCACCCGTCGTTGCCGGAAAATCAGGGGCATGAAATTGCCGCGCGCCAGCAAAAAGGCTTTGGCGCAATGTTGGGTTTTGAACTGGAT                                                                                 >  SRR4302537.6004242/1‑94 (MQ=60)
               CTGTATCACCCGTCGTTGCCGGAAAATCAGGGGCATGAAATTGCCGCGCGCCAGCAAAAAGGCTTTGGCGCAATGTTGGGTTTTGAACTGGATG                                                                                <  SRR4302537.3361463/94‑1 (MQ=60)
                TGTATCACCCGTCGTTGCCGGAAAATCAGGGGCATGAAATTGCCGCGCGCCAGCAAAAAGGCTTTGGCGCAATGTTGGGTTTTGAACTGGATGG                                                                               >  SRR4302537.1942539/1‑94 (MQ=60)
                TGTATCACCCGTCGTTGCCGGAAAATCAGGGGCATGAAATTGCCGCGCGCCAGCAAAAAGGCTTTGGCGCAATGTTGGGTTTTGAACTGGATGG                                                                               <  SRR4302537.867819/94‑1 (MQ=60)
                TGTATCACCCGTCGTTGCCGGAAAATCAGGGGCATGAAATTGCCGCGCGCCAGCAAAAAGGCTTTGGCGCAATGTTGGGTTTTGAACTGGATGG                                                                               <  SRR4302537.2534552/94‑1 (MQ=60)
                 GTATCACCCGTCGTTGCCGGAAAATCAGGGGCATGAAATTGCCGCGCGCCAGCAAAAAGGCTTTGGCGCAATGTTGGGTTTTGAACTGGATGGC                                                                              <  SRR4302537.685738/94‑1 (MQ=60)
                 GTATCACCCGTCGTTGCCGGAAAATCAGGGGCATGAAATTGCCGCGCGCCAGCAAAAAGGCTTTGGCGCAATGTTGGGTTTTGAACTGGATGGC                                                                              >  SRR4302537.1139762/1‑94 (MQ=60)
                 GTATCACCCGTCGTTGCCGGAAAATCAGGGGCATGAAATTGCCGCGCGCCAGCAAAAAGGCTTTGGCGCAATGTTGGGTTTTGAACTGGATGGC                                                                              <  SRR4302537.280278/94‑1 (MQ=60)
                  TATCACCCGTCGTTGCCGGAAAATCAGGGGCATGAAATTGCCGCGCGCCAGCAAAAAGGCTTTGGCGCAATGTTGGGTTTTGAACTGGATGGCG                                                                             <  SRR4302537.1028722/94‑1 (MQ=60)
                    TCACCCGTCGTTGCCGGAAAATCAGGGGCATGAAATTGCCGCGCGCCAGCAAAAAGGCTTTGGCGCAATGTTGGGTTTTGAACTGGATGGCGAT                                                                           >  SRR4302537.3330752/1‑94 (MQ=60)
                     CACCCGTCGTTGCCGGAAAATCAGGGGCATGAAATTGCCGCGCGCCAGCAAAAAGGCTTTGGCGCAATGTTGGGTTTTGAACTGGATGGCGATG                                                                          >  SRR4302537.6048221/1‑94 (MQ=60)
                      ACCCGTCGTTGCCGGAAAATCAGGGGCATGAAATTGCCGCGCGCCAGCAAAAAGGCTTTGGCGCAATGTTGGGTTTTGAACTGGATGGCGATGA                                                                         >  SRR4302537.144926/1‑94 (MQ=60)
                       CCCGTCGTTGCCGGAAAATCAGGGTCATGAAATTGCCGCGCGCCAGCAAAAAGGCTTTGGCGCAATGTTGGGTTTTGAACTGGATGGCGATGAG                                                                        <  SRR4302537.6106534/94‑1 (MQ=60)
                        CCGTCGTTGCCGGAAAATCAGGGGCATGAAATTGCCGCGCGCCAGCAAAAAGGCTTTGGCGCAATGTTGGGTTTTGAACTGGATGGCGATGAGC                                                                       >  SRR4302537.4568784/1‑94 (MQ=60)
                        CCGTCGTTGCCGGAAAATCAGGGGCATGAAATTGCCGCGCGCCAGCAAAAAGGCTTTGGCGCAATGTTGGGTTTTGAACTGGATGGCGATGAGC                                                                       <  SRR4302537.5487826/94‑1 (MQ=60)
                         CGTCGTTGCCGGAAAATCAGGGGCATGAAATTGCCGCGCGCCAGCAAAAAGGCTTTGGCGCAATGTTGGGTTTTGAACTGGATGGCGATGAGCA                                                                      >  SRR4302537.5963292/1‑94 (MQ=60)
                          GTCGTTGCCGGAAAATCAGGGGCATGAAATTGCCGCGCGCCAGCAAAAAGGCTTTGGCGCAATGTTGGGTTTTGAACTGGATGGCGATGAGCAG                                                                     >  SRR4302537.5251431/1‑94 (MQ=60)
                          GTCGTTGCCGGAAAATCAGGGGCATGAAATTGCCGCGCGCCAGCAAAAAGGCTTTGGCGCAATGTTGGGTTTTGAACTGGATGGCGATGAGCAG                                                                     <  SRR4302537.5078366/94‑1 (MQ=60)
                          GTCGTTGCCGGAAAATCAGGGGCATGAAATTGCCGCGCGCCAGCAAAAAGGCTTTGGCGCAATGTTGGGTTTTGAACTGGATGGCGATGAGCAG                                                                     >  SRR4302537.801559/1‑94 (MQ=60)
                          GTCGTTGCCGGAAAATCAGGGGCATGAAATTGCCGCGCGCCAGCAAAAAGGCTTTGGCGCAATGTTGGGTTTTGAACTGGATGGCGATGAGCAG                                                                     <  SRR4302537.3016609/94‑1 (MQ=60)
                           TCGTTGCCGGAAAATCAGGGGCATGAAATTGCCGCGCGCCAGCAAAAAGGCTTTGGCGCAATGTTGGGTTTTGAACTGGATGGCGATGAGCAGA                                                                    <  SRR4302537.897801/94‑1 (MQ=60)
                             GTTGCCGGAAAATCAGGGGCATGAAATTGCCGCGCGCCAGCAAAAAGGCTTTGGCGCAATGTTGGGTTTTGAACTGGATGGCGATGAGCAGACG                                                                  >  SRR4302537.427491/1‑94 (MQ=60)
                              TTGCCGGAAAATCAGGGGCATGAAATTGCCGCGCGCCAGCAAAAAGGCTTTGGCGCAATGTTGGGTTTTGAACTGGATGGCGATGAGCAGACGC                                                                 >  SRR4302537.3283513/1‑94 (MQ=60)
                               TGCCGGAAAATCAGGGGCATGAAATTGCCGCGCGCCAGCAAAAAGGCTTTGGCGCAATGTTGGGTTTTGAACTGGATGGCGATGAGCAGACGCT                                                                >  SRR4302537.6002359/1‑94 (MQ=60)
                                   GGAAAATCAGGGGCATGAAATTGCCGCGCGCCAGCAAAAAGGCTTTGGCGCAATGTTGGGTTTTGAACTGGATGGCGATGAGCAGACGCTGCGT                                                            >  SRR4302537.1303764/1‑94 (MQ=60)
                                    GAAAATCAGGGGCATGAAATTGCCGCGCGCCAGCAAAAAGGCTTTGGCGCAATGTTGGGTTTTGAACTGGATGGCGATGAGCAGACGCTGCGTC                                                           <  SRR4302537.5206399/94‑1 (MQ=60)
                                    GAAAATCAGGGGCATGAAATTGCCGCGCGCCAGCAAAAAGGCTTTGGCGCAATGTTGGGTTTTGAACTGGATGGCGATGAGCAGACGCTGCGTC                                                           <  SRR4302537.5479694/94‑1 (MQ=60)
                                     AAAATCAGGGGCATGAAATTGCCGCGCGCCAGCAAAAAGGCTTTGGCGCAATGTTGGGTTTTGAACTGGATGGCGATGAGCAGACGCTGCGTCG                                                          >  SRR4302537.6435696/1‑94 (MQ=60)
                                     AAAATCAGGGGCATGAAATTGCCGCGCGCCAGCAAAAAGGCTTTGGCGCAATGTTGAGTTTTGAACTGGATGGCGATGAGCAGACGCTGCGTCG                                                          <  SRR4302537.2782548/94‑1 (MQ=60)
                                       AATCAGGGGCATGAAATTGCCGCGCGCCAGCAAAAAGGCTTTGGCGCAATGTTGGGTTTTGAACTGGATGGCGATGAGCAGACGCTGCGTCGTT                                                        >  SRR4302537.2582747/1‑94 (MQ=60)
                                        ATCAGGGGCATGAAATTGCCGCGCGCCAGCAAAAAGGCTTTGGCGCAATGTTGGGTTTTGAACTGGATGGCGATGAGCAGGCGCTGCGTCGTTT                                                       <  SRR4302537.2135517/94‑1 (MQ=60)
                                        ATCAGGGGCATGAAATTGCCGCGCGCCAGCAAAAAGGCTTTGGCGCAATGTTGGGTTTTGAACTGGATGGCGATGAGCAGACGCTGCGTCGTTT                                                       >  SRR4302537.6041548/1‑94 (MQ=60)
                                        ATCAGGGGCATGAAATTGCCGCGCGCCAGCAAAAAGGCTTTGGCGCAATGCTGGGTTTTGAACTGGATGGCGATGAGCAGACGCTGCGTCGTTT                                                       <  SRR4302537.4582547/94‑1 (MQ=60)
                                        ATCAGGGGCATGAAATTGCCGCGCGCAAGCAAAAAGGCTTTGGCGCAATGTTGGGTTTTGAACTGGATGGCGATGAGCAGACGCTGCGTCGTTT                                                       <  SRR4302537.1820110/94‑1 (MQ=60)
                                         TCAGGGGCATGAAATTGCCGCGCGCCAGCAAAAAGGCTTTGGCGCAATGTTGGGTTTTGAACTGGATGGCGATGAGCAGACGCTGCGTCGTTTC                                                      <  SRR4302537.1666698/94‑1 (MQ=60)
                                          CAGGGGCATGAAATTGCCGCGCGCCAGCAAAAAGGCTTTGGCGCAATGTTGGGTTTTGAACTGGATGGCGATGAGCAGACGCTGCGTCGTTTCC                                                     >  SRR4302537.6327934/1‑94 (MQ=60)
                                           AGGGGCATGAAATTGCCGCGCGCCAGCAAAAAGGCTTTGGCGCAATGTTGGGTTTTGAACTGGATGGCGATGAGCAGACGCTGCGTCGTTTCCT                                                    <  SRR4302537.75712/94‑1 (MQ=60)
                                             GGGCATGAAATTGCCGCGCGCCAGCAAAAAGGCTTTGGCGCAATGTTGGGTTTTGAACTGGCTGGCGATGAGCAGACGCTGCGTCGTTTCCTGG                                                  >  SRR4302537.2885143/1‑94 (MQ=60)
                                             GGGCATGAAATTGCCGCGCGCCAGCAAAAAGGCTTTGGCGCAATGTTGGGTTTTGAACTGGATGGCGATGAGCAGACGCTGCGTCGTTTCCTGG                                                  <  SRR4302537.3641872/94‑1 (MQ=60)
                                             GGGCATGAAATTGCCGCGCGCCAGCAAAAAGGCTTTGGCGCAATGTTGGGTTTTGAACTGGATGGCGATGAGCAGACGCTGCGTCGTTTCCTGG                                                  >  SRR4302537.4652397/1‑94 (MQ=60)
                                             GGGCATGAAATTGCCGCGCGCCAGCAAAAAGGCTTTGGCGCAATGTTGGGTTTTGAACTGGATGGCGATGAGCAGACGCTGCGTCGTTTCCTGG                                                  >  SRR4302537.2885141/1‑94 (MQ=60)
                                                CATGAAATTGCCGCGCGCCAGCAAAAAGGCTTTGGCGCAATGTTGGGTTTTGAACTGGATGGCGATGAGCAGACGCTGCGTCGTTTCCTGGGCG                                               <  SRR4302537.1977017/94‑1 (MQ=60)
                                                  TGAAATTGCCGCGCGCCAGCAAAAAGGCTTTGGCGCAATGTTGGGTTTTGAACTGGATGGCGATGAGCAGACGCTGCGTCGTTTCCTGGGCGGG                                             <  SRR4302537.6297612/94‑1 (MQ=60)
                                                  TGAAATTGCCGCGCGCCAGCAAAAAGGCTTTGGCGCAATGTTGGGTTTTGAACTGGATGGCGATGAGCAGACGCTGCGTCGTTTCCTGGGCGGG                                             >  SRR4302537.5656028/1‑94 (MQ=60)
                                                  TGAAATTGCCGCGCGCCAGCAAAAAGGCTTTGGCGCAATGTTGGGTTTTGAACTGGATGGCGATGAGCAGACGCTGCGTCGTTTCCTGGGCGGG                                             >  SRR4302537.102508/1‑94 (MQ=60)
                                                   GAAATTGCCGCGCGCCAGCAAAAAGGCTTTGGCGCAATGTTGGGTTTTGAACTGGATGGCGATGAGCAGACGCTGCGTCGTTTCCTGGGCGGGC                                            >  SRR4302537.1046477/1‑94 (MQ=60)
                                                   GAAATTGCCGCGCGCCAGCAAAAAGGCTTTGGCGCAATGTTGAGTTTTGAACTGGATGGCGATGAGCAGACGCTGCGTCGTTTCCTGGGCGGGC                                            >  SRR4302537.5071979/1‑94 (MQ=60)
                                                     AATTGCCGCGCGCCAGCAAAAAGGCTTTGGCGCAATGTTGGGTTTTGAACTGGATGGCGATGAGCAGACGCTGCGTCGTTTCCTGGGCGGGCTG                                          >  SRR4302537.2238084/1‑94 (MQ=60)
                                                     AATTGCCGCGCGCCAGCAAAAAGGCTTTGGCGCAATGTTGGGTTTTGAACTGGATGGCGATGAGCAGACGCTGCGTCGTTTCCTGGGCGGGCTG                                          <  SRR4302537.3295751/94‑1 (MQ=60)
                                                       TTGCCGCGCGCCAGCAAAAAGGCTTTGGCGCAATGTTGGGTTTTGAACTGGATGGCGATGAGCAGACGCTGCGTCGTTTCCTGGGCGGGCTGTC                                        <  SRR4302537.5470733/94‑1 (MQ=60)
                                                        TGCCGCGCGCCAGCAAAAAGGCTTTGGCGCAATGTTGGGTTTTGAACTGGATGGCGATGAGCAGACGCTGCGTCGTTTCCTGGGCGGGCTGTCG                                       >  SRR4302537.5564103/1‑94 (MQ=60)
                                                          GCGCGCGCCAGCAAAAAGGCTTTGGCGCAATGTTGGGTTTTGAACTGGATGGCGATGGGCAGACGCTGCGTCGTTTCCTGGGCGGGCTGTCGTT                                     <  SRR4302537.4654130/94‑1 (MQ=60)
                                                          CCGCGCGCCAGCAAAAAGGCTTTGGCGCAATGTTGGGTTTTGAACTGGATGGCGATGAGCAGACGCTGCGTCGTTTCCTGGGCGGGCTGTCGTT                                     >  SRR4302537.87137/1‑94 (MQ=60)
                                                             CGCGCCAGCAAAAAGGCTTTGGCGCAATGTTGGGTTTTGAACTGGATGGCGATGAGCAGACGCTGCGTCGTTTCCTGGGCGGGCTGTCGTTGTT                                  <  SRR4302537.6162765/94‑1 (MQ=60)
                                                             CGCGCCAGCAAAAAGGCTTTGGCGCAATGTTGGGTTTTGAACTGGATGGCGATGAGCAGACGCTGCGTCGTTTCCTGGGCGGGCTGTCGTTGTT                                  <  SRR4302537.893434/94‑1 (MQ=60)
                                                               CGCCAGCAAAAAGGCTTTGGCGCAATGTTGGGTTTTGAACTGGATGGCGATGAGCAGACGCTGCGTCGTTTCCTGGGCGGGCTGTCGTTGTTTA                                >  SRR4302537.654882/1‑94 (MQ=60)
                                                                GCCAGCAAAAAGGCTTTGGCGCAATGTTGGGTTTTGAACTGGATGGCGATGAGCAGACGCTGCGTCGTTTCCTGGGCGGGCTGTCGTTGTTTAC                               <  SRR4302537.3806557/94‑1 (MQ=60)
                                                                GCCAGCAAAAAGGCTTTGGCGCAATGTTGGGTTTTGAACTGGATGGCGATGAGCAGACGCTGCGTCGTTTCCTGGGCGGGCTGTCGTTGTTTAC                               <  SRR4302537.2706551/94‑1 (MQ=60)
                                                                 CCAGCAAAAAGGCTTTGGCGCAATGTTGGGTTTTGAACTGGATGGCGATGAGCAGACGCTGCGTCGTTTCCTGGGCGGGCTGTCGTTGTTTACG                              <  SRR4302537.5520094/94‑1 (MQ=60)
                                                                  CAGCAAAAAGGCTTTGGCGCAATGTTGGGTTTTGAACTGGATGGCGATGAGCAGACGCTGCGTCGTTTCCTGGGCGGGCTGTCGTTGTTTACGC                             >  SRR4302537.2219340/1‑94 (MQ=60)
                                                                  CAGCAAAAAGGCTTTGGCGCAATGTTGGGTTTTGAACTGGATGGCGATGAGCAGACGCTGCGTCGTTTCCTGGGCGGGCTGTCGTTGTTTACGC                             <  SRR4302537.1768132/94‑1 (MQ=60)
                                                                  CAGCAAAAAGGCTTTGGCGCAATGTTGGGTTTTGAACTGGATGGCGATGAGCAGACGCTGCGTCGTTTCCTGGGCGGGCTGTCGTTGTTTACGC                             >  SRR4302537.2464377/1‑94 (MQ=60)
                                                                  CAGCAAAAAGGCTTTGGCGCAATGTTGAGTTTTGAACTGGATGGCGATGAGCAGACGCTGCGTCGTTTCCTGGGCGGGCTGTCGTTGTTTACGC                             >  SRR4302537.1374698/1‑94 (MQ=60)
                                                                   AGCAAAAAGGCTTTGGCGCAATGTTGGGTTTTGAACTGGATGGCGATGAGCAGACGCTGCGTCGTTTCCTGGGCGGGCTGTCGTTGTTTACGCT                            <  SRR4302537.1846974/94‑1 (MQ=60)
                                                                    GCAAAAAGGCTTTGGCGCAATGTTGGGTTTTGAACTGGATGGCGATGAGCAGACGCTGCGTCGTTTCCTGGGCGGGCTGTCGTTGTTTACGCTG                           <  SRR4302537.3636074/94‑1 (MQ=60)
                                                                    GCAAAAAGGCTTTGGCGCAATGTTGAGTTTTGAACTGGATGGCGATGAGCAGACGCTGCGTCGTTTCCTGGGCGGGCTGTCGTTGTTTACGCTG                           <  SRR4302537.2600419/94‑1 (MQ=60)
                                                                     CAAAAAGGCTTTGGCGCAATGTTGAGTTTTGAACTGGATGGCGATGAGCAGACGCTGCGTCGTTTCCTGGGCGGGCTGTCGTTGTTTAAGCTGG                          >  SRR4302537.1007506/1‑94 (MQ=60)
                                                                      AAAAAGGCTTTGGCGCAATGTTGGGTTTTGAACTGGATGGCGATGAGCAGACGCTGCGTCGTTTCCTGGGCGGGCTGTCGTTGTTTACGCTGGC                         <  SRR4302537.6333387/94‑1 (MQ=60)
                                                                      AAAAAGGCTTTGGCGCAATGTTGGGTTTTGAACTGGATGGCGATGAGCAGACGCTGCGTCGTTTCCTGGGCGGGCTGTCGTTGTTTACGCTGGC                         >  SRR4302537.2843213/1‑94 (MQ=60)
                                                                      AAAAAGGCTTTGGCGCAATGTTGAGTTTTGAACTGGATGGCGATGAGCAGACGCTGCGTCGTTTCCTGGGCGGGCTGTCGTTGTTTACGCTGGG                         >  SRR4302537.3156192/1‑94 (MQ=60)
                                                                       AAAAGGCTTTGGCGCGATGTGGGGTGTTGAGCTGGATGGCGATGAGCAGACGCTGCGTCGTTTCCTGGGCGGGCTGTCGTTGTTTACGCTGGCG                        <  SRR4302537.491935/94‑1 (MQ=60)
                                                                       AAAAGGCTTTGGCGCAATGTTGGGTTTTGAACTGGATGGCGATGAGCAGACGCTGCGTCGTTTCCTGGGCGGGCTGTCGTTGTTTACGCTGGCG                        >  SRR4302537.3648259/1‑94 (MQ=60)
                                                                       AAAAGGCTTTGGCGCAATGTTGGGTTTTGAACTGGATGGCGATGAGCAGACGCTGCGTCGTTTCCTGGGCGGGCTGTCGTTGTTTACGCTGGCG                        >  SRR4302537.6287605/1‑94 (MQ=60)
                                                                       AAAAGGCTTTGGCGCAATGTTGGGTTTTGAACTGGATGGCGATGAGCAGACGCTGCGTCGTTTCCTGGGCGGGCTGTCGTTGTTTACGCTGGCG                        <  SRR4302537.6140348/94‑1 (MQ=60)
                                                                       AAAAGGCTTTGGCGCAATGTTGGGTTTTGAACTGGATGGCGATGAGCAGACGCTGCGTCGTTTCCTGGGCGGGCTGTCGTTGTTTACGCTGGCG                        <  SRR4302537.1279556/94‑1 (MQ=60)
                                                                       AAAAGGCTTTGGCGCAATGTTGGGTTTTGAACTGGATGGCGATGAGCAGACGCTGCGTCGTTTCCTGGGCGGGCTGTCGTTGTTTACGCTGGCG                        >  SRR4302537.4463468/1‑94 (MQ=60)
                                                                       AAAAGGCTTTGGCGCAATGTTGGGTTTTGAACTGGATGGCGATGAGCAGACGCTGCGTCGTTTCCTGGGCGGGCTGTCGTTGTTTACGCTGGCG                        >  SRR4302537.5969788/1‑94 (MQ=60)
                                                                          AGGCTTTGGCGCAATGTTGGGTTTTGAACTGGATGGCGATGAGCAGACGCTGCGTCGTTTCCTGGGCGGGCTGTCGTTGTTTACGCTGGCGGAA                     <  SRR4302537.5526695/94‑1 (MQ=60)
                                                                           GGCTTTGGCGCAATGTTGGGTTTTGAACTGGATGGCGATGAGCAGACGCTGCGTCGTTTCCTGGGCGGGCTGTCGTTGTTTACGCTGGCGGAAT                    >  SRR4302537.5043933/1‑94 (MQ=60)
                                                                           GGCTTTGGCGCAATGTTGGGTTTTGAACTGGATGGCGATGAGCAGACGCTGCGTCGTTTCCTGGGCGGGCTGTCGTTGTTTACGCTGGCGGAAT                    >  SRR4302537.3125583/1‑94 (MQ=60)
                                                                            GCTTTGGCGCAATGTTGGGTTTTGAACTGGATGGCGATGAGCAGACGCTGCGTCGTTTCCTGGGCGGGCTGTCGTTGTTTACGCTGGCGGAATC                   <  SRR4302537.2050870/94‑1 (MQ=60)
                                                                             CTTTGGCGCAATGTTGGGTTTTGAACTGGATGGCGATGAGCAGACGCTGCGTCGTTTCCTGGGCGGGCTGTCGTTGTTTACGCTGGCGGAATCA                  >  SRR4302537.269215/1‑94 (MQ=60)
                                                                             CTTTGGCGCAATGTTGGGTTTTGAACTGGAGGGCGATGAGCAGACGCTGCGTCGTTTCCTGGGCGGGCTGTCGTTGTTTACGCTGGCGGAATCA                  <  SRR4302537.6197240/94‑1 (MQ=60)
                                                                                TGGCGCAATGTTGGGTTTTGAACTGGATGGCGATGAGCAGACGCTGCGTCGTTTCCTGGGCGGGCTGTCGTTGTTTACGCTGGCGGAATCATTA               <  SRR4302537.4057929/94‑1 (MQ=60)
                                                                                TGGCGCAATGTTGGGTTTTGAACTGGATGGCGATGAGCAGACGCTGCGTCGTTTCCTGGGCGGGCTGTCGTTGTTTACGCTGGCGGAATCATTA               <  SRR4302537.4092960/94‑1 (MQ=60)
                                                                                 GGCGCAATGTTGGGTTTTGAACTGGATGGCGATGAGCAGACGCTGCGTCGTTTCCTGGGCGGGCTGTCGTTGTTTACGCTGGCGGAATCATTAG              <  SRR4302537.623188/94‑1 (MQ=60)
                                                                                 GGCGCAATGTTGGGTTTTGAACTGGATGGCGATGAGCAGACGCTGCGTCGTTTCCTGGGCGGGCTGTCGTTGTTTACGCTGGCGGAATCATTAG              >  SRR4302537.5149061/1‑94 (MQ=60)
                                                                                   CGCAATGTTGGGTTTTGAACTGGATGGCGATGAGCAGACGCTGCGTCGTTTCCTGGGCGGGCTGTCGTTGTTTACGCTGGCGGAATCATTAGGG            >  SRR4302537.6080701/1‑94 (MQ=60)
                                                                                   CGCAATGTTGGGTTTTGAACTGGATGGCGATGAGCAGACGCTGCGTCGTTTCCTGGGCGGGCTGTCGTTGTTTACGCTGGCGGAATCATTAGGG            >  SRR4302537.4974876/1‑94 (MQ=60)
                                                                                   CGCAATGTTGGGTTTTGAACTGGATGGCGATGAGCAGACGCTGCGTCGTTTCCTGGGCGGGCTGTCGTTGTTTACGCTGGCGGAATCATTAGGG            <  SRR4302537.2294899/94‑1 (MQ=60)
                                                                                     CAATGTTGGGTTTTGAACTGGATGGCGATGAGCAGACGCTGCGTCGTTTCCTGGGCGGGCTGTCGTTGTTTACGCTGGCGGAATCATTAGGGGG          >  SRR4302537.928756/1‑94 (MQ=60)
                                                                                     CAATGTTGGGTTTTGAACTGGATGGCGATGAGCAGACGCTGCGTCGTTTCCTGGGCGGGCTGTCGTTGTTTACGCTGGCGGAATCATTAGGGGG          <  SRR4302537.4925006/94‑1 (MQ=60)
                                                                                      AATGTTGGGTTTTGAACTGGATGGCGATGAGCAGACGCTGCGTCGTTTCCTGGGCGGGCTGTCGTTGTTTACGCTGGCGGAATCATTAGGGGGG         >  SRR4302537.5657427/1‑94 (MQ=60)
                                                                                       ATGTTGGGTTTTGAACTGGATGGCGATGTGCAGACGCTGCGTCGTTTCCTGGGCGGGCTGTCGTTGTTTACGCTGGCGGAATCATTAGGGGGAG        >  SRR4302537.2359773/1‑94 (MQ=60)
                                                                                       ATGTTGGGTTTTGAACTGGATGGCGATGAGCAGACGCTGCGTCGTTTCCTGGGCGGGCTGTCGTTGTTTACGCTGGCGGAATCATTAGGGGGAG        <  SRR4302537.1804712/94‑1 (MQ=60)
                                                                                        TGTTTGGTTTTGAACTGGATGGCGATGAGCAGACGCTGCGTCGTTTCCTGGGCGGGCTGTCGTTGTTTACGCTGGCGGAATCATTAGGGGGAGT       <  SRR4302537.163240/94‑1 (MQ=60)
                                                                                         GTTGGGTTTTGAACTGGATGGCGATGAGCAGACGCTGCGTCGTTTCCTGGGCGGGCTGTCGTTGTTTACGCTGGCGGAATCATTAGGGGGAGTG      >  SRR4302537.674734/1‑94 (MQ=60)
                                                                                         GTTGGGTTTTGAACTGGATGGCGATGAGCAGACGCTGCGTCGTTTCCTGGGCGGGCTGTCGTTGTTTACGCTGGCGGAATCATTAGGGGGAGTG      >  SRR4302537.3084147/1‑94 (MQ=60)
                                                                                          TTGGGTTTTGAACTGGATGGCGATGAGCAGACGCTGCGTCGTTTCCTGGGCGGGCTGTCGTTGTTTACGCTGGCGGAATCATTAGGGGGAGTGG     >  SRR4302537.2482816/1‑94 (MQ=60)
                                                                                          TTGGGTTTTGAACTGGATGGCGATGAGCAGACGCTGCGTCGTTTCCTGGGCGGGCTGTCGTTGTTTACGCTGGCGGAATCATTAGGGGGAGTGG     <  SRR4302537.1680531/94‑1 (MQ=60)
                                                                                             GGTTTTGAACTGGATGGCGATGAGCAGACGCTGCGTCGTTTCCTGGGCGGGCTGTCGTTGTTTACGCTGGCGGAATCATTAGGGGGAGTGGAAA  <  SRR4302537.2510103/94‑1 (MQ=60)
                                                                                             |                                                                                             
CCGTTGGTGAAAAAACTGTATCACCCGTCGTTGCCGGAAAATCAGGGGCATGAAATTGCCGCGCGCCAGCAAAAAGGCTTTGGCGCAATGTTGAGTTTTGAACTGGATGGCGATGAGCAGACGCTGCGTCGTTTCCTGGGCGGGCTGTCGTTGTTTACGCTGGCGGAATCATTAGGGGGAGTGGAAA  >  NC_000913/4127059‑4127245

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 7 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑